Real-time PCR solutions from sample preparation to data analysis

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1 PRODUCT OVERVIEW Real-time PCR Real-time PCR solutions from sample preparation to data analysis For over a quarter entury, we ve provided you with reliable reagents, instruments and software for all your qpcr workflow needs. Whether you re doing sample preparation, reverse transription, amplifiation and detetion using Applied Biosystems TM TaqMan TM or Applied Biosystems TM SYBR TM hemistry on our real-time PCR instruments, or data analysis, you an ontinue to trust us to support your researh with high-quality produts and servie designed to help you get onsistently exellent data. Sample preparation Gene Expression Cells-to-C T Kit Easily perform expression analysis using ultured ells without purifiation of RNA. Reagents are inluded for ell lysis and RNA preservation, genomi DNA removal and reverse transription. Ambion mirvana mirna Isolation Kit Isolate total RNA ontaining small RNAs suh as mirna, sirna and snrna. This kit inludes reagents to enrih for small RNA (<200bp), enabling sensitive detetion of low levels of small RNAs. Applied Biosystems Arturus PioPure kits Isolate RNA or extrat DNA from laser-apture mirodisseted (LCM) samples. Easily perform expression analysis from as little as a single LCM ell. Applied Biosystems Protein Expression Sample Preparation Kit Extrat proteins from your target and ontrol ell lysates for use with Protein Assays. Sample-to-SNP Kit Offers a streamlined protool for performing TaqMan hemistry based genotyping analysis on any sample. Applied Biosystems MagMAX DNA Multi-Sample Kit Designed for rapid purifiation of high-quality genomi DNA from a variety of sample types, inluding blood and blood ards, tissue, mouse tails, bual swabs, buffy oats and ultured ells. Applied Biosystems Arturus Paradise kits Isolate RNA from formalin-fixed, paraffin-embedded (FFPE) laser-apture mirodisseted (LCM) samples.

2 TaqMan Assays by appliation Gene expression analysis Gene Expression Assays offer 1.3 million primer/ probe sets for 24 speies and seleted pathogens, with your hoie of Applied Biosystems FAM or VIC dye labels. Applied Biosystems Custom Plus Assay kits and standard ustom assay kits enable you to study the expression of any gene or splie variant in any organism. Drug metabolism genotyping Choose from over 2,700 unique Applied Biosystems TaqMan Drug Metabolism SNP Genotyping Assays to detet polymorphisms in all known high-value DME pharmaogeneti markers, inluding pharmaadme ore markers. SNP genotyping Choose from 7 million predesigned human and mouse SNP Genotyping Assays, or Custom SNP Genotyping Assays, in various sizes. Protein expression Revolutionary Protein Assays enable fast, easy identifi ation and relative quantifi ation of protein markers from limited quantities of ultured ells. Choose from predesigned assays, or reate your own using your antibodies. MiroRNA and nonoding RNA analysis Innovative Assay based produts and related tools, for mirna and nonoding RNA researh from isolation through disovery. Long nonoding RNA (lnrna) quantifi ation. mirna profi ling. pri-mirna quantifi ation. Small RNA quantifi ation. Targeted mirna quantifi ation. Applied Biosystems TaqMan MiroRNA Assays using mirna-speifi RT primer. Applied Biosystems TaqMan Advaned mirna Assays using universal RT primer. Somati mutation detetion In addition, our Applied Biosystems Custom TaqMan Assay Design Tool lets you design and order a TaqMan Assay for gene expression or genotyping appliations. Custom assay design servies are available for any other design request. For more information, go to thermofisher.om/taqman Choie of formats Assays are available in single-tube format for maximum fl exibility or an be plated in three different formats to minimize your effort and ost: Applied Biosystems TaqMan Array Plates for quik setup help to minimize human error; Array Cards for ease of use in automation; and OpenArray Plates for high throughput and lower ost per sample. Choose between predefi ned panels, fl exible-ontent panels or ustom options to design your panel. Single-tube assays TaqMan Array Plates (96- and 384-well) TaqMan Array Cards OpenArray Plates

3 Multiplex qpcr Solution Multiplex qpcr is a simple, effiient and ost-effetive solution for overoming the hallenges of limited samples and ostly analysis. Our TaqMan Multiplex Solution onsists of an ustom probe with QSY quenher, ABY and JUN dyes, and an optimized Multiplex qpcr Master Mix using MUSTANG PURPLE dye as a passive referene. This solution enables you to perform up to a 4-plex reation for gene expression and a duplex reation for genotyping analysis on an Applied Biosystems real-time PCR instrument. Learn more at thermofisher.om/multiplexqpr Reverse transription Invitrogen SuperSript VILO DNA Synthesis Kit Provides high-quality reverse transription, a streamlined workflow with maximum sensitivity and dynami range. Advaned mirna DNA Synthesis Kit Provides universal RT hemistry to prepare DNA template for use with TaqMan Advaned mirna Assays. MiroRNA Reverse Transription Kit Contains omponents for optimal performane of TaqMan MiroRNA Assays. Contents of this kit are used with the RT primer provided with the TaqMan MiroRNA Assay to onvert mirna to DNA. Master mixes for real-time PCR amplifiation Fast Advaned Master Mix Superior performane for both routine and hallenging real-time PCR experiments, whih an be used in both standard and fast reations and has exellent benhtop stability. Universal Master Mix II Brings sensitivity and preision aross a broad range of input target quantities, reliable detetion of low opy number targets and aurate quantifiation to disriminate subtle differenes in target abundane. PreAmp Master Mix Preamplifies small amounts of DNA without introduing amplifiation bias to the sample and strethes preious sample into many more real-time PCR reations. Genotyping Master Mix Obtain superior luster resolution for lear SNP alleli disrimination. Optimized for endpoint fluoresene detetion in genotyping appliations. Multiplex Master Mix TaqMan Multiplex Master Mix is formulated to amplify up to four targets in a single reation, delivering shorter run times. Applied Biosystems PowerUp SYBR Green Master Mix Formulated for maximum speifiity and reproduibility. The proprietary Dual-Lok Taq DNA Polymerase enzyme uses a ombination of two hot-start mehanisms to ontrol its ativity. Tight reproduibility over a broad dynami range. Compatible with fast or standard yling. One universal master mix for all qpcr instruments.

4 Applied Biosystems Power SYBR Green Master Mix Superior sensitivity and reproduibility without ompromising performane aross all real-time PCR systems. Protein Assay Core Reagents Kit with Master Mix Performs ligation and PCR steps for protein expression. Fast Virus 1-Step Master Mix Designed for reliable, highly sensitive DNA and RNA virus detetion in ommonly used sample types, even in the presene of ommon reation inhibitors. A single-tube format allows for uniform handling and proessing. Real-time PCR systems QuantStudio 3 Real-Time PCR System A small, affordable solution onneting you to your data like never before. Designed to be the easiest to use and most interative instrument, offering the high performane analysis expeted from Applied Biosystems instruments. Easy-to-use intuitive software design enables easy experiment setup and an interative system allows you to get results faster. Diretly onnet to Thermo Fisher Cloud to aess and analyze your data anytime, from anywhere with an internet onnetion. Bak up and share files to ollaborate with olleagues worldwide. VeriFlex tehnology provides three independent temperature zones for preise ontrol over your qpcr optimization. Fatory alibrated for optial and thermal auray, the instrument is delivered ready for quik installation and use. Applied Biosystems QuantStudio 5 Real-Time PCR System A high-performane benhtop instrument giving you greater ontrol of your experimental data. Interat with the latest advanements in touh-sreen usability, aess your data more easily than ever before and seurely share your results with ollaborators around the world. Multiplex with ease six exitation filters and six emission filters offer 21 different olor ombinations, allowing a broad range of detetion hemistries and maximum multiplexing. Diretly onnet to Thermo Fisher Cloud to aess and analyze your data anytime, from anywhere, with an internet onnetion. Bak up and share files to ollaborate with olleagues worldwide. VeriFlex tehnology provides six independent temperature zones for preise ontrol over your PCR optimization.* Have onfidene in your reords built-in seurity, auditing and eletroni signature (SAE) pakage omes standard on the QuantStudio 5 system to assist you in 21 CFR Part 11 ompliane. Protet your work with individual user aounts and the ability to lok a protool template giving you peae of mind that only you ontrol your runs. 384-well format inludes 5 olors; no VeriFlex Bloks temperature ontrol. Applied Biosystems QuantStudio 6 Flex Real-Time PCR System Ideal for laboratories with multiple appliations or end-users on a limited budget. With an upgradable path to an Applied Biosystems QuantStudio 7 Flex Real-Time PCR System to aommodate automation or TaqMan Array Cards, the QuantStudio 6 Flex system is the ideal qpcr platform if your needs hange in the future. With one instrument, you an interhange between 96-well, 96-well Fast and 384-well formats. The intuitive software, easy touh-sreen setup and effortless blok hange are designed to let you get started right away.

5 Detet as small as 1.5-fold hanges in singleplex reations and with a 10-log dynami range. Can be seamlessly upgraded to a QuantStudio 7 Flex system by a field servie engineer to give you additional automation, throughput and multiplexing apabilities. QuantStudio 7 Flex Real-Time PCR System Delivers the proven reliability, sensitivity and auray of the Applied Biosystems ViiA 7 Real-Time System in a new industrial design. The QuantStudio 7 Flex system is optimized to enable the broadest range of quantitative PCR appliations. Run hundreds of real-time PCR reations effortlessly using TaqMan Array Cards integrated with the Twister Automation Robot. The QuantStudio 7 Flex system an maximize your throughput apabilities for automated environments. Optimized protools, reagents and intuitive software are available for the broadest range of appliations. Get improved well-to-well and instrument-toinstrument data auray with the OptiFlex System, featuring six deoupled exitation and emission filter hannels, with 21 filter ombinations for maximum multiplexing and hemistry flexibility. Applied Biosystems QuantStudio 12K Flex Real-Time PCR System Designed for ultimate throughput, flexibility and salability, the QuantStudio 12K Flex system provides a single platform that enables you to perform targeted disovery through onfirmation and sreening, as well as many other appliations. Platform runs 96-well, Fast 96-well and 384-well plates, as well as TaqMan Array Card and OpenArray Plate formats. Five interhangeable thermal yling bloks enable one to 12,000 data points per run. Enhaned OptiFlex System with white-light LED and 21 filter ombinations for maximum hemistry options. Advaned software for gene expression and mirorna profiling, genotyping and digital PCR. Find out more at thermofisher.om/quantstudioqprfamily Free data analysis software Applied Biosystems qpcr Analysis Modules A fast, powerful, flexible online data analysis solution that ontains analysis module options for omparative C t (also known as relative quantifiation), absolute quantifiation and genotyping. Applied Biosystems qpcr Analysis Modules offer inreased analysis apaity and flexible features above our existing software solutions. Genotyper Software For use with TaqMan SNP Genotyping Assays (predesigned, ustom and DME) in ombination with 48-, 96- and 384-well mirotiter plates and Applied Biosystems OpenArray Genotyping Plates. It offers a state-of-the-art genotype-alling algorithm, an intuitive user interfae and enhaned multi-plate analysis features. DataAssist Software An easy-to-use data analysis tool that utilizes the omparative C t (ΔΔC t ) method to rapidly and aurately quantify relative gene expression aross a large number of genes and samples.

6 Digital PCR Need to extend the apabilities of real-time PCR? Digital PCR is a new approah to nulei aid detetion and quantifiation that uses moleular ounting. It offers an alternate method to onventional real-time quantitative PCR for absolute quantifiation and rare-allele detetion without the use of a standard urve. A simple and affordable platform, the Applied Biosystems QuantStudio 3D Digital PCR System produes absolute quantifiation data with the preision and sensitivity you need for your more demanding appliations. Find out more at thermofisher.om/quantstudio3d For more details about all these produts and more, go to thermofisher.om/taqman For Researh Use Only. Not for use in diagnosti proedures Thermo Fisher Sientifi In. All rights reserved. All trademarks are the property of Thermo Fisher Sientifi and its subsidiaries unless otherwise speified. TaqMan is a registered trademark of Rohe Moleular Systems, In., used under permission and liense. Twister is a registered trademark of Caliper Life Sienes, In. CO