Approaches that can be used to study expression of specific proteins
|
|
|
- Julianna Morton
- 10 years ago
- Views:
Transcription
1 Approaches that can be used to study expression of specific proteins Receptors and transporters Homogenate binding studies Receptor autoradiography Radiochemical Western blotting Immunohistochemistry/cytochemistry Immunochemical
2 Approaches that can be used to study expression of specific proteins Enzymes Immunhistochemical/cytochemical analysis/immunoblotting for the presence of enzyme molecules Enzyme assay functional activity
3 Approaches that can be used to study expression of specific proteins Transcription factors Expression using Western blotting Expression using immunohistochemistry Phosphorylation state (CREB vs pcreb) Western blotting/elisa DNA binding (EMSA assay)/elisa based DNA binding assays Determine cytosolic vs. nuclear location?
4 Measurement of protein in brain tissue Bradford assay dye binding assay Measure protein in: - Tissue homogenate Analyse data Generic Skills: - Pipetting skills - Serial dilutions - Data analysis Equalize tissue for protein concentation
5 Western immunoblotting for a specific protein of interest
6 Southern Northern DNA RNA Transcription Splicing Mature mrna Nucleus Cytoplasm Translation Western Proteins
7 7 Steps- Western/Protein Blotting Tissue Preperation Protein Determination SDS-polyacrylamide gel electrophoresis Blotting Labeling Detection Quantification
8 Tissue Preparation/Extraction of protein Homogenisation Homogenization Buffer: Protease inhibitors prevent the breakdown of the polypeptide chain by proteases Phosphatase inhibitors inhibit protein dephosphorylation DDT, EDTA breakdown various bonds Sucrose gives weight to sample Centrifugation Protein determination v. important: comparisons are made as per total amount of protein present per well
9 Tissue preparation (ctd) Sample added 1:1 with sample buffer Sample buffer contains: SDS: Breaks down quaternary, tertiary, and secondary structures of proteins. β-mercaptoethanol breaks the disulphide bonds in multisubunit proteins. Bromophenol Blue Dye: allows monitoring of electrophoretic run. Glycerol: gives sample weight
10 Electrophoresis Study of the movement of charged molecules in an electric field (Faraday 1833: passage of electricity through solution). Uses: Analysis and purification of biomolecules especially proteins, nucleic acids, peptides and nucleotides. May also be used to determine molecular weights Particles migrate through a solvent that is supported be a homogenous medium (eg) gel. Gel acts as a sieve. Gel types: Polyacrylamide/agarose/agarose-acrylamide Polyacryamide molecular sieving can be controlled by the concentration of the gel and the absorption of proteins is minimal.
11 Polyacrylamide Gels Are formed from the polymerisation of acrylamide monomer is the presence of smaller amounts of N,N - methylene-bis acrylamide (bis-acrylamide) bis-acrylamide=crosslinking agent. Polymerised in a head to tail fashion which is initialised by ammonium persulfate (APS) and TEMED.
12 SDS-PAGE (Maizel, 1966) At a neutral ph, in 1% SDS and 0.1 M mercaptoethanol (within gel), most multichain proteins bind SDS and dissociate. Complexes consisting of protein subunits and SDS behave as though they have uniform shape and an identical charge-to-mass ratio.
13 SDS-PAGE (ctd) Overall charge is negative due to binding of SDS Proteins migrate towards the anode due to negative charge. The proteins then separate according to size with the gel acting as a molecular sieve. Pore size of the gel is variable (% acrylamide) Low percentage i.e. high pore size is required for high molecular weight proteins and vice versa
14 SDS PAGE Denaturing electrophoresis -Multisubunit proteins give multiple bands on a gel
15 Blotting Pore size of the gel limits penetration by antibodies Blotting onto a porous membrane can be achieved giving a mirror image of the gel. Nitrocellulose: versatile, easy to use, no activation required, brittle when dry PVDF: no blow through of proteins, good mechanical strength, requires immersion in methanol
16 Labeling & Detection Light Hyper film-ecl
17 Densitometry converts band density into numbers Densitometry Sample immunoblot
Protein immunoblotting
Protein immunoblotting (Western blotting) Dr. Serageldeen A. A. Sultan Lecturer of virology Dept. of Microbiology SVU, Qena, Egypt [email protected] Western blotting -It is an analytical technique used to
Electrophoresis and Electroblotting of Proteins
Electrophoresis and Electroblotting of Proteins The purpose of the next lab exercises will be to study the relative amounts of β-actin in cells of the B-16 melanoma, liver and muscle of mice. Electrophoresis
Pharmaceutical Biotechnology. Recombinant DNA technology Western blotting and SDS-PAGE
Pharmaceutical Biotechnology Recombinant DNA technology Western blotting and SDS-PAGE Recombinant DNA Technology Protein Synthesis Western Blot Western blots allow investigators to determine the molecular
Chapter 3 Contd. Western blotting & SDS PAGE
Chapter 3 Contd. Western blotting & SDS PAGE Western Blot Western blots allow investigators to determine the molecular weight of a protein and to measure relative amounts of the protein present in different
EZ-Run Protein Gel Solution. EZ-Run Protein Standards. EZ-Run Gel Staining Solution. Traditional SDS-Page Reagents. Protein Electrophoresis
EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-Page Reagents Protein Electrophoresis protein electrophoresis Introduction Sodium dodecyl sulfate polyacrylamide
Discontinuous native protein gel electrophoresis
Discontinuous native protein gel electrophoresis Michael Niepmann and Junfeng Zheng Institute of Biochemistry Friedrichstrasse 24 Faculty of Medicine, JustusLIebigUniversity 35392 Giessen, Germany In this
Western Blotting. USA: [email protected] UK & Europe: [email protected] China: [email protected]. www.ptglab.com
Western Blotting All steps are carried out at room temperature unless otherwise indicated. Recipes for all solutions highlighted bold are included at the end of the protocol. SDS-PAGE 1. Construct an SDS-PAGE
Western Blotting: Mini-gels
Western Blotting: Mini-gels Materials a Protein Extraction Buffer (for callus or kernel), Solution Stock Final Volume Tris-HCl ph 80 1 M 200 mm 20 ml NaCl 4 M 100 mm 25 ml Sucrose 2 M 400 mm 20 ml EDTA
Detection of proteins by lithium dodecyl sulphate polyacrylamide gel electrophoresis
Detection of proteins by lithium dodecyl sulphate polyacrylamide gel electrophoresis During electrophoretic measurements a mixture of compounds in solution is taken into a chamber, two electrodes are joined
Biology 309 Lab Notebook
Name: Biology 309 Lab Notebook This is a guided lab notebook for you to keep well-organized notes about procedures and record experimental data for experiments as they are performed. It is guided because,
SDS-PAGE. (June 23, 2005)
SDS-PAGE (June 23, 2005) GATHER REAGENTS & MATERIALS 30% acrylamide/0.8% bisacrylamide (30:1) 4X Tris. Cl/SDS, ph 8.8 4X Tris. Cl/SDS, ph 6.8 Ammonium persulfate, 10% (Make fresh each time.) SDS electrophoresis
Immunoblotting (Western blotting)
Immunoblotting (Western blotting) HIV Transfer to NC membrane membrane gel support buffer Dissociate in SDS Incubate with 1ary antiserum Separate by SDS-PAG Incubate w/ labeled 2nd ab Immunoblotting :
Running protein gels and detection of proteins
Running protein gels and detection of proteins 1. Protein concentration determination using the BIO RAD reagent This assay uses a colour change reaction to give a direct measurement of protein concentration.
Biochemistry Lab SDS PAGE and Western blot General Instructions
Background When an electrical field is applied across a solution, the movement of the charged particles (proteins) is influenced not only by the charge but also the voltage, distance between electrodes,
DNA Separation Methods. Chapter 12
DNA Separation Methods Chapter 12 DNA molecules After PCR reaction produces many copies of DNA molecules Need a way to separate the DNA molecules from similar sized molecules Only way to genotype samples
2007 7.013 Problem Set 1 KEY
2007 7.013 Problem Set 1 KEY Due before 5 PM on FRIDAY, February 16, 2007. Turn answers in to the box outside of 68-120. PLEASE WRITE YOUR ANSWERS ON THIS PRINTOUT. 1. Where in a eukaryotic cell do you
WESTERN BLOTTING TIPS AND TROUBLESHOOTING GUIDE TROUBLESHOOTING GUIDE
WESTERN BLOTTING TIPS AND TROUBLESHOOTING GUIDE TIPS FOR SUCCESSFUL WESTERB BLOTS TROUBLESHOOTING GUIDE 1. Suboptimal protein transfer. This is the most common complaint with western blotting and could
PROTOCOL 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 www.mitosciences.com
PROTOCOL Western Blotting Transfer and Detection Procedure 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 02-11 DESCRIPTION Western Blotting Transfer and Detection Procedure ADDITIONAL MATERIALS REQUIRED
Lecture 8. Protein Trafficking/Targeting. Protein targeting is necessary for proteins that are destined to work outside the cytoplasm.
Protein Trafficking/Targeting (8.1) Lecture 8 Protein Trafficking/Targeting Protein targeting is necessary for proteins that are destined to work outside the cytoplasm. Protein targeting is more complex
7 Electrophoresis. µ proportional to Q
7 Electrophoresis Objectives: A) To perform agarose gel electrophoresis of the proteins isolated in last week's experiment and B) to interpret the banding patterns produced by these proteins. Introduction:
Western Blotting. Prepare samples:
Western Blotting Sive Lab Protocol March 2007 Prepare samples: For zebrafish embryos: Option 1: Take live embryos and put into 1.5 ml tube with E3. Centrifuge gently for 1-2 minutes -yolk lipids will rise
Molecular Genetics. RNA, Transcription, & Protein Synthesis
Molecular Genetics RNA, Transcription, & Protein Synthesis Section 1 RNA AND TRANSCRIPTION Objectives Describe the primary functions of RNA Identify how RNA differs from DNA Describe the structure and
Section XIII: Protein Separation in Agarose Gels
Section XIII: In This Section Introduction 196 Buffers for in Agarose 197 Casting Agarose Gels for 198 Preparation and Loading of Protein Samples 198 Optimal Voltage and Electrophoretic Times 199 Detection
A Practical Approach on SDS PAGE for Separation of Protein
A Practical Approach on SDS PAGE for Separation of Protein Suvra Roy, Vikash Kumar* Central Inland Fisheries Research Institute (CIFRI), Barrackpore, 700120, India Abstract: Polyacrylamide gel electrophoresis
Pure-IP Western Blot Detection Kit
Product Manual Pure-IP Western Blot Detection Kit Catalog Number PRB-5002 20 blots FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction The technique of immunoprecipitation (IP) is used
Classic Immunoprecipitation
292PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 [email protected] A Geno Technology, Inc. (USA) brand name Classic Immunoprecipitation Utilizes Protein A/G Agarose for Antibody Binding (Cat.
METHOD USED TO EXTRACT TOTAL MUSCLE PROTEIN FOR WESTERN BLOT USING TRIS-EDTA BUFFER*
METHOD USED TO EXTRACT TOTAL MUSCLE PROTEIN FOR WESTERN BLOT USING TRIS-EDTA BUFFER* SOLUTIONS FOR SAMPLE EXTRACTION 1. Tris-EDTA Buffer, ph 8.3 1 liter 50 mm Tris 6.06 g 10 mm EDTA 3.72 g Adjust ph to
Molecular Cell Biology. Prof. D. Karunagaran. Department of Biotechnology. Indian Institute of Technology Madras
Molecular Cell Biology Prof. D. Karunagaran Department of Biotechnology Indian Institute of Technology Madras Module 5 Methods in Cell Biology (Methods to Manipulate Protein, DNA and RNA and Methods to
Protocol for Western Blotting
Protocol for Western Blotting Materials Materials used on Day 3 Protease inhibitor stock: 1 μg/μl pepstatin A in DMSO 200 μm leupeptin in OG Buffer 200 mm PMSF: Freshly made. Ex) 34.8 mg PMSF in 1 ml isopropanol
The Steps. 1. Transcription. 2. Transferal. 3. Translation
Protein Synthesis Protein synthesis is simply the "making of proteins." Although the term itself is easy to understand, the multiple steps that a cell in a plant or animal must go through are not. In order
2. Cut 6 sheets of Whatman 3MM paper and 1 sheet of blotting membrane to the size of the gel, or slightly smaller.
Method for Western Blotting Western Blotting ELECTROPHORESIS Prepare and run an SDS PAGE gel. Select a gel percent which will give the best resolution for the size of antigen being analyzed (if known).
Chapter 3. Protein Structure and Function
Chapter 3 Protein Structure and Function Broad functional classes So Proteins have structure and function... Fine! -Why do we care to know more???? Understanding functional architechture gives us POWER
Proteins and Nucleic Acids
Proteins and Nucleic Acids Chapter 5 Macromolecules: Proteins Proteins Most structurally & functionally diverse group of biomolecules. : o Involved in almost everything o Enzymes o Structure (keratin,
Protein transfer from SDS-PAGE to nitrocellulose membrane using the Trans-Blot SD cell (Western).
Western Blot SOP Protein transfer from SDS-PAGE to nitrocellulose membrane using the Trans-Blot SD cell (Western). Date: 8/16/05, 10/31/05, 2/6/06 Author: N.Oganesyan, R. Kim Edited by: R. Kim Summary:
Western Blot Analysis with Cell Samples Grown in Channel-µ-Slides
Western Blot Analysis with Cell Samples Grown in Channel-µ-Slides Polyacrylamide gel electrophoresis (PAGE) and subsequent analyses are common tools in biochemistry and molecular biology. This Application
Methods for Protein Analysis
Methods for Protein Analysis 1. Protein Separation Methods The following is a quick review of some common methods used for protein separation: SDS-PAGE (SDS-polyacrylamide gel electrophoresis) separates
WESTERN BLOTTING - A BEGINNER S GUIDE
WESTERN BLOTTING - A BEGINNER S GUIDE Western blotting identifies with specific antibodies proteins that have been separated from one another according to their size by gel electrophoresis. The blot is
How To Understand The Chemistry Of Organic Molecules
CHAPTER 3 THE CHEMISTRY OF ORGANIC MOLECULES 3.1 Organic Molecules The chemistry of carbon accounts for the diversity of organic molecules found in living things. Carbon has six electrons, four of which
Chapter 14 SDS-PAGE. Objectives
SDS-PAGE This lab will introduce you to SDS-PAGE, a simple and inexpensive method for resolving proteins in complex mixtures. SDS-PAGE gels provide the starting materials for western blots and for some
Chapter 5: The Structure and Function of Large Biological Molecules
Name Period Concept 5.1 Macromolecules are polymers, built from monomers 1. The large molecules of all living things fall into just four main classes. Name them. 2. Circle the three classes that are called
Lecture Overview. Hydrogen Bonds. Special Properties of Water Molecules. Universal Solvent. ph Scale Illustrated. special properties of water
Lecture Overview special properties of water > water as a solvent > ph molecules of the cell > properties of carbon > carbohydrates > lipids > proteins > nucleic acids Hydrogen Bonds polarity of water
Genetic information (DNA) determines structure of proteins DNA RNA proteins cell structure 3.11 3.15 enzymes control cell chemistry ( metabolism )
Biology 1406 Exam 3 Notes Structure of DNA Ch. 10 Genetic information (DNA) determines structure of proteins DNA RNA proteins cell structure 3.11 3.15 enzymes control cell chemistry ( metabolism ) Proteins
6 Characterization of Casein and Bovine Serum Albumin
6 Characterization of Casein and Bovine Serum Albumin (BSA) Objectives: A) To separate a mixture of casein and bovine serum albumin B) to characterize these proteins based on their solubilities as a function
RNA & Protein Synthesis
RNA & Protein Synthesis Genes send messages to cellular machinery RNA Plays a major role in process Process has three phases (Genetic) Transcription (Genetic) Translation Protein Synthesis RNA Synthesis
Definitive guide to western blot
Definitive guide to western blot Western blotting uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose
Electrophoresis EXPERIMENT 4. Theory
( F EXPERIMENT 4 Electrophoresis Theory Basic Principles Electrophoresis is the process of migration of charged molecules through solutions in an applied electric field. Electrophoresis is often classified
NuPAGE Technical Guide
Instruction Manual NuPAGE Technical Guide General information and protocols for using the NuPAGE electrophoresis system Version E October 1, 2003 IM-1001 2 Table of Contents Table of Contents...3 General
Chapter 2 Antibodies. Contents. Introduction
Chapter 2 Antibodies Keywords Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytochemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining
The Molecules of Cells
The Molecules of Cells I. Introduction A. Most of the world s population cannot digest milk-based foods. 1. These people are lactose intolerant because they lack the enzyme lactase. 2. This illustrates
NO CALCULATORS OR CELL PHONES ALLOWED
Biol 205 Exam 1 TEST FORM A Spring 2008 NAME Fill out both sides of the Scantron Sheet. On Side 2 be sure to indicate that you have TEST FORM A The answers to Part I should be placed on the SCANTRON SHEET.
Understanding the immune response to bacterial infections
Understanding the immune response to bacterial infections A Ph.D. (SCIENCE) DISSERTATION SUBMITTED TO JADAVPUR UNIVERSITY SUSHIL KUMAR PATHAK DEPARTMENT OF CHEMISTRY BOSE INSTITUTE 2008 CONTENTS Page SUMMARY
Transcription and Translation of DNA
Transcription and Translation of DNA Genotype our genetic constitution ( makeup) is determined (controlled) by the sequence of bases in its genes Phenotype determined by the proteins synthesised when genes
Laboratory Techniques I Oncology for Scientists I
Laboratory Techniques I Oncology for Scientists I September 9 th, 2015 Hayley Affronti, PhD Student [email protected] Dr. Sheila Figel too! When we first hit the lab there are so many things
PRACTICE TEST QUESTIONS
PART A: MULTIPLE CHOICE QUESTIONS PRACTICE TEST QUESTIONS DNA & PROTEIN SYNTHESIS B 1. One of the functions of DNA is to A. secrete vacuoles. B. make copies of itself. C. join amino acids to each other.
serum protein and A/ G ratio
serum protein and A/ G ratio Blood plasma contains at least 125 individual proteins. Serum ( as contrasted with plasma) is deficient in those coagulation protein which are consumed during the process of
Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a
Optimal Conditions for F(ab ) 2 Antibody Fragment Production from Mouse IgG2a Ryan S. Stowers, 1 Jacqueline A. Callihan, 2 James D. Bryers 2 1 Department of Bioengineering, Clemson University, Clemson,
Chemical Basis of Life Module A Anchor 2
Chemical Basis of Life Module A Anchor 2 Key Concepts: - Water is a polar molecule. Therefore, it is able to form multiple hydrogen bonds, which account for many of its special properties. - Water s polarity
CHRISTIAN LAB WESTERN BLOT PROTOCOL
CHRISTIAN LAB WESTERN BLOT PROTOCOL There is actually 2 parts to a western blot: A. SDS-PAGE: Separates protein by size. Smaller proteins migrate faster through the gel than larger proteins. Size separation
From DNA to Protein. Proteins. Chapter 13. Prokaryotes and Eukaryotes. The Path From Genes to Proteins. All proteins consist of polypeptide chains
Proteins From DNA to Protein Chapter 13 All proteins consist of polypeptide chains A linear sequence of amino acids Each chain corresponds to the nucleotide base sequence of a gene The Path From Genes
Chapter 5. The Structure and Function of Macromolecule s
Chapter 5 The Structure and Function of Macromolecule s Most Macromolecules are polymers: Polymer: (poly: many; mer: part) Large molecules consisting of many identical or similar subunits connected together.
AP BIOLOGY 2008 SCORING GUIDELINES
AP BIOLOGY 2008 SCORING GUIDELINES Question 1 1. The physical structure of a protein often reflects and affects its function. (a) Describe THREE types of chemical bonds/interactions found in proteins.
Protein extraction from Tissues and Cultured Cells using Bioruptor Standard & Plus
Protein extraction from Tissues and Cultured Cells using Bioruptor Standard & Plus Introduction Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical
Developing Protocols of Tricine-SDS-PAGE for Separation of Polypeptides in the Mass Range 1-30 kda with Minigel Electrophoresis System
Int. J. Electrochem. Sci., 11 (2016) 640-649 International Journal of ELECTROCHEMICAL SCIENCE www.electrochemsci.org Developing Protocols of Tricine-SDS-PAGE for Separation of Polypeptides in the Mass
Electrophoresis Guide
Novex Pre-Cast Gel Electrophoresis Guide Version B January 27, 2003 IM-1002 Novex Pre-Cast Gel Electrophoresis Guide General information and protocols for using Novex pre-cast gels www.invitrogen.com [email protected]
Western Blotting For Protein Analysis
Western Blotting For Protein Analysis Part 1: Laemmli Gel Electrophoresis Using Mini-PROTEAN II Electrophoresis Cell Note: Powder-free gloves should be worn throughout the entire procedure. A. Preparing
Disaccharides consist of two monosaccharide monomers covalently linked by a glycosidic bond. They function in sugar transport.
1. The fundamental life processes of plants and animals depend on a variety of chemical reactions that occur in specialized areas of the organism s cells. As a basis for understanding this concept: 1.
Carbohydrates, proteins and lipids
Carbohydrates, proteins and lipids Chapter 3 MACROMOLECULES Macromolecules: polymers with molecular weights >1,000 Functional groups THE FOUR MACROMOLECULES IN LIFE Molecules in living organisms: proteins,
The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small
The immune response Antibodies Antigens Epitopes (antigenic determinants) the part of a protein antigen recognized by an antibody Haptens small molecules that can elicit an immune response when linked
Ready Gel System RESOURCE GUIDE
Ready Gel System RESOURCE GUIDE Ready Gel System Resource Guide Exploring, discovering, and understanding the world around us - the biological systems interacting with the environment - is a process that
Name: Hour: Elements & Macromolecules in Organisms
Name: Hour: Elements & Macromolecules in Organisms Most common elements in living things are carbon, hydrogen, nitrogen, and oxygen. These four elements constitute about 95% of your body weight. All compounds
Biological molecules:
Biological molecules: All are organic (based on carbon). Monomers vs. polymers: Monomers refer to the subunits that, when polymerized, make up a larger polymer. Monomers may function on their own in some
Gel Electrophoresis of Proteins
AES Application Focus Gel Electrophoresis of Proteins Page 1 Gel Electrophoresis of Proteins Adapted from Chapter 7, Gel Electrophoresis of Proteins, by David E. Garfin, Pages 197-268, in Essential Cell
1. The diagram below represents a biological process
1. The diagram below represents a biological process 5. The chart below indicates the elements contained in four different molecules and the number of atoms of each element in those molecules. Which set
A Guide to Protein Blotting
A Guide to Protein Blotting SDS-PAGE SDS-PAGE stands for sodium dodecyl (lauryl) sulphate-polyacrylamide gel electrophoresis. SDS-PAGE has a number of uses, which include: establishing protein size, protein
GENE REGULATION. Teacher Packet
AP * BIOLOGY GENE REGULATION Teacher Packet AP* is a trademark of the College Entrance Examination Board. The College Entrance Examination Board was not involved in the production of this material. Pictures
A disaccharide is formed when a dehydration reaction joins two monosaccharides. This covalent bond is called a glycosidic linkage.
CH 5 Structure & Function of Large Molecules: Macromolecules Molecules of Life All living things are made up of four classes of large biological molecules: carbohydrates, lipids, proteins, and nucleic
SDS-PAGE Protocol Mutated from the SDS-PAGE protocol written by the Lord of the Flies
SDS-PAGE Protocol Mutated from the SDS-PAGE protocol written by the Lord of the Flies Pouring the resolving gel 1. Clean glass plates with soap and water, then with ethanol. Assemble the glass plates and
Troubleshooting Guide for DNA Electrophoresis
Troubleshooting Guide for Electrophoresis. ELECTROPHORESIS Protocols and Recommendations for Electrophoresis electrophoresis problem 1 Low intensity of all or some bands 2 Smeared bands 3 Atypical banding
Technical Manual No. 0210 Update date 10112010
Express TM PAGE Gels Technical Manual No. 0210 Update date 10112010 I Description.. 1 II Gel Selection Guide. 1 III Protein Migration Table.. 2 IV Compatible Gel Boxes... 2 V Related Products. 3 VI Storage.
Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION
Molecular Biology Techniques: A Classroom Laboratory Manual THIRD EDITION Susan Carson Heather B. Miller D.Scott Witherow ELSEVIER AMSTERDAM BOSTON HEIDELBERG LONDON NEW YORK OXFORD PARIS SAN DIEGO SAN
#01 Polyacrylamide Slab Gel Electrophoresis of Proteins Hassan Ahmad, University of Texas-Pan American, Edinburg, TX 78539
#01 Polyacrylamide Slab Gel Electrophoresis of Proteins Hassan Ahmad, University of Texas-Pan American, Edinburg, TX 78539 INTRODUCTION Description Electrophoresis involves the transport of charged molecules
Dynamics in nanoworlds
Dynamics in nanoworlds Interplay of energy, diffusion and friction in (sub)cellular world 1 NB Queste diapositive sono state preparate per il corso di Biofisica tenuto dal Dr. Attilio V. Vargiu presso
Carbon-organic Compounds
Elements in Cells The living substance of cells is made up of cytoplasm and the structures within it. About 96% of cytoplasm and its included structures are composed of the elements carbon, hydrogen, oxygen,
DP419 RNAsimple Total RNA Kit. RNAprep pure Series. DP501 mircute mirna Isolation Kit. DP438 MagGene Viral DNA / RNA Kit. DP405 TRNzol Reagent
Overview of TIANGEN Products DP419 RNAsimple Total RNA Kit DP430 RNAprep pure Kit(For Cell/Bacteria) DP315/DP315-R TIANamp Virus DNA/RNA Kit DP431 RNAprep pure Kit (For Tissue) Silica-membrane Technology
Protein Synthesis How Genes Become Constituent Molecules
Protein Synthesis Protein Synthesis How Genes Become Constituent Molecules Mendel and The Idea of Gene What is a Chromosome? A chromosome is a molecule of DNA 50% 50% 1. True 2. False True False Protein
Structure and Function of DNA
Structure and Function of DNA DNA and RNA Structure DNA and RNA are nucleic acids. They consist of chemical units called nucleotides. The nucleotides are joined by a sugar-phosphate backbone. The four
First Strand cdna Synthesis
380PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 [email protected] A Geno Technology, Inc. (USA) brand name First Strand cdna Synthesis (Cat. # 786 812) think proteins! think G-Biosciences
How To Perform A Western Blot
Introduction to Western Blotting Principles Technical Guidance Data Analysis Troubleshooting Contents Chapter 1 Introduction Overview... 5 Why Use a Western Blot?.... 6 Antibody Considerations... 7 Monoclonals
Anti-ATF6 α antibody, mouse monoclonal (1-7)
Anti-ATF6 α antibody, mouse monoclonal (1-7) 73-500 50 ug ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress.
13.2 Ribosomes & Protein Synthesis
13.2 Ribosomes & Protein Synthesis Introduction: *A specific sequence of bases in DNA carries the directions for forming a polypeptide, a chain of amino acids (there are 20 different types of amino acid).
Elements & Macromolecules in Organisms
Name: Date: Per: Table # Elements & Macromolecules in rganisms Most common elements in living things are carbon, hydrogen, nitrogen, and oxygen. These four elements constitute about 95% of your body weight.
Semiquantitative Western Blot (quantification of SMN protein levels)
Please quote this SOP in your Methods. Semiquantitative Western Blot (quantification of SMN protein levels) SOP (ID) Number SMA_M.1.2.006 Version 2 Issued December 20 th, 2010 Last reviewed January 14
Worksheet 13.1. Chapter 13: Human biochemistry glossary
Worksheet 13.1 Chapter 13: Human biochemistry glossary α-helix Refers to a secondary structure of a protein where the chain is twisted to form a regular helix, held by hydrogen bonds between peptide bonds
The molecules of life. The molecules that make up living things are really big They are called macromolecules
Food Labels All living things use materials and energy Our food comes from living things The food labels we see show us what our food is made of The stuff we are studying today can be found on food labels
How To Make A Tri Reagent
TRI Reagent For processing tissues, cells cultured in monolayer or cell pellets Catalog Number T9424 Store at room temperature. TECHNICAL BULLETIN Product Description TRI Reagent is a quick and convenient
10.1 The function of Digestion pg. 402
10.1 The function of Digestion pg. 402 Macromolecules and Living Systems The body is made up of more than 60 % water. The water is found in the cells cytoplasm, the interstitial fluid and the blood (5