Murat Günaydın Pubmed yayın listesi
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1 Murat Günaydın Pubmed yayın listesi Int J Antimicrob Agents Feb;41(2):199. doi: /j.ijantimicag Epub 2012 Nov 20. Investigation of biofilm formation in Acinetobacter baumannii isolates and their colistin susceptibilities in biofilm. Milletli Sezgin F, Coban AY, Gunaydin M. Ondokuz Mayis University, Medical School, Department of Medical Microbiology, Samsun, Turkey. PMID: [PubMed - in process] BMC Infect Dis Feb 16;12:42. doi: / Comparative activity of carbapenem testing (the COMPACT study) in Turkey. Leblebicioglu H, Cakir N, Celen M, Kurt H, Baris H, Laeuffer J; Turkish COMPACT Study Group. Collaborators: Akata F, Yuluğkural Z, Ayaz C, Çelebri G, Cömert F, Gerçeker D, Gülay Z, Günaydin M, Kaya A, Ersöz G, Özer S, Benzononana N, Taşova Y, Yaman A. Ondokuzmayis University, Kurupelit Kampüsü, Samsun, Turkey. hakanomu@omu.edu.tr BACKGROUND: Recent evidence indicates that Gram-negative bacterial pathogens, the most common of which are Pseudomonas spp., Enterobacteriaceae, and Acinetobacter baumannii, are frequent causes of hospital-acquired infections. This study aims to evaluate the in vitro activity of doripenem and comparator carbapenem antibiotics against Gram-negative clinical isolates collected from COMParative Activity of Carbapenem Testing (COMPACT) study centres in Turkey. METHODS: Ten centres in Turkey were invited to submit Pseudomonas aeruginosa, Enterobacteriaceae, and other Gram-negative isolates from intensive care unit (ICU)/non-ICU patients with complicated intra-abdominal infections, bloodstream infections, or nosocomial pneumonia, including ventilator-associated pneumonia, between May and October Susceptibility was determined by each centre using E-test. A central laboratory performed species confirmation as well as limited susceptibility and quality-control testing. RESULTS: Five hundred and ninety six isolates were collected. MIC90 values for doripenem, meropenem, and imipenem, respectively, were 32, 64, and 64 mg/l against Pseudomonas spp.; 0.12, 0.12, and 0.5 mg/l against Enterobacteriaceae; and 64 mg/l for each against other Gram-negative isolates. In determining the susceptibility of hospital isolates of selected Gram-negative pathogens to doripenem, imipenem, and meropenem, we found that against all pathogens combined, the MIC90 for ICU compared with non-icu isolates was higher. CONCLUSIONS: Doripenem showed similar or slightly better activity than meropenem and better activity than imipenem against the Gram-negative pathogens collected in Turkey. PMCID: PMC
2 PMID: [PubMed - indexed for MEDLINE] Clin Vaccine Immunol Jun;18(6): doi: /CVI Epub 2011 Apr 20. Serotypes of Streptococcus pneumoniae isolates from children with invasive pneumococcal disease in Turkey: baseline evaluation of the introduction of the pneumococcal conjugate vaccine nationwide. Ceyhan M, Gurler N, Yaman A, Ozturk C, Oksuz L, Ozkan S, Keser M, Salman N, Alhan E, Esel D, Gultekin M, Camcioglu Y, Gul M, Sorguc Y, Aydemir S, Gunaydin M, Yakupogullari Y, Kizirgil A. Hacettepe University, Faculty of Medicine, Department of Pediatric Infectious Diseases, Sihhiye, 06100, Ankara, Turkey. mceyhan@hacettepe.edu.tr Before use of the pneumococcal conjugate vaccine PCV7 became widespread in Turkey, 202 invasive pneumococcus isolates were analyzed. The most common serotypes were 19F and 6B. In children 2 years of age, the potential coverage rate of PCV7 was 69.5%. The most frequent non-pcv7 serotypes were 19A, 3, 1, 6A, and 8. PMCID: PMC PMID: [PubMed - indexed for MEDLINE] Ann Clin Microbiol Antimicrob Mar 6;8:7. doi: / Are we aware how contaminated our mobile phones with nosocomial pathogens? Ulger F, Esen S, Dilek A, Yanik K, Gunaydin M, Leblebicioglu H. Department of Anesthesiology and Reanimation, Faculty of Medicine, Ondokuz Mayis University, Kurupelit, Samsun, Turkey. faulger@gmail.com Erratum in Ann Clin Microbiol Antimicrob. 2009;8:31. BACKGROUND: The objective of this study was to determine the contamination rate of the healthcare workers' (HCWs') mobile phones and hands in operating room and ICU. Microorganisms from HCWs' hands could be transferred to the surfaces of the mobile phones during their use. METHODS: 200 HCWs were screened; samples from the hands of 200 participants and 200 mobile phones were cultured. RESULTS: In total, 94.5% of phones demonstrated evidence of bacterial contamination with different types of bacteria. The gram negative strains were isolated from mobile phones of 31.3% and the ceftazidime resistant strains from the hands were 39.5%. S. aureus strains isolated from mobile phones of 52% and those strains isolated from hands of 37.7% were methicillin resistant. Distributions of the isolated microorganisms from mobile phones were similar to hands isolates. Some mobile phones were contaminated with nosocomial important pathogens. CONCLUSION: These results showed that HCWs' hands and their mobile phones were
3 contaminated with various types of microorganisms. Mobile phones used by HCWs in daily practice may be a source of nosocomial infections in hospitals. PMCID: PMC PMID: [PubMed - indexed for MEDLINE] Indian J Med Microbiol Oct;25(4): Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals. Hoşoğlu S, Gündes S, Kolayli F, Karadenizli A, Demirdağ K, Günaydin M, Altindis M, Caylan R, Ucmak H. Department of Infectious Diseases and Clinical Microbiology, Dicle University Hospital, Diyarbakir, Turkey. hosoglu@hotmail.com PURPOSE: To study the prevalence of TEM-, SHV- and GES-type beta -lactamases among Escherichia coli and Klebsiella pneumoniae strains having ceftazidime MICs higher than 2 mg/l. METHODS: A total of 63 E. coli and 41 K. pneumoniae isolated from five different university hospitals were studied for the existence of TEM-, SHV- and GES-type beta -lactamases. Susceptibility tests were carried out according to the criteria of National Committee for Clinical Laboratory Standards. MICs were obtained by agar dilution method. Existence of extended-spectrum beta -lactamases (ESBLs) were assessed by double-disc synergy test (DDST). Existence of the above-mentioned beta -lactamase genes were studied both by PCR with specific oligonucleotide primers and isoelectric focusing methods. RESULTS: None of the isolates were carbapenem-resistant. DDSTs were positive in 50 (79.3%) and 33 (80.5%) of E. coli and K. pneumoniae, respectively. TEM gene was detected in 41 (65.1%) and 19 (46.3%), whereas SHV gene in 18 (28.6%) and 20 (48.8%) of E. coli and K. pneumoniae strains, respectively. GES genes were not detected. CONCLUSIONS: TEM and SHV genes are highly prevalent among ESBL-producing E. coli and K. pneumoniae, whereas GES-type ESBLs are absent and found not to be responsible of ceftazidime resistance in Turkish hospitals. PMID: [PubMed - indexed for MEDLINE] J Virol Methods Aug;119(2): Distinguishing hepatitis B virus (HBV) genotype D from non-d by a simple PCR. Eroglu C, Leblebicioglu H, Gunaydin M, Turan D, Sunbul M, Esen S, Sanic A. Department of Clinical Microbiology and Infectious Diseases, School of Medicine, Ondokuz Mayis University, Samsun 55139, Turkey. drcafer@hotmail.com Different HBV genotypes have characteristic geographical distribution, which is important epidemiologically. HBV strains have been classified into eight different genotypes (A-H) on the basis of >8% differences in the entire genomic
4 sequence. Genotypes A and D are predominant in Europe, Africa, and the USA, genotypes B and C are restricted to East Asia, genotype E is found in Africa, and genotype F is found in indigenous populations in Central and South America. Genotype D is prevalent in the Turkish population. HBV genotype D shows a 33-bp deletion in the pre-s1 region that accounts for their smaller genomic size (3182 bp). This deletion can be used to facilitate the identification of genotype D. A primer in the pre-s1 region was designed to discriminate genotype D from non-d by PCR. Sixty genotype D (40 acute and 20 chronic) and 4 genotype A sera identified by restriction fragment length polymorphism (RFLP) were included in the study. Using this simple PCR method, all genotype D sera were identified correctly and the test was able to detect HBV DNA at 1000 genomes per ml. An advantage of this method is that it can differentiate in a mixture of genotypes (genotype D from non-d) provided that one isn't present below 1 x 10(4) copies/ml. In conclusion this method is rapid (approximately 5h) and it will contribute to the epidemiological study of HBV in high prevalence areas of genotype D. It can also differentiate between genotype D from non-d in cases of co-infection. PMID: [PubMed - indexed for MEDLINE] J Chemother Apr;14(2): Physicians' antibiotic prescribing habits for upper respiratory tract infections in Turkey. Leblebicioglu H, Canbaz S, Peksen Y, Gunaydin M. Department of Infectious Diseases and Clinical Microbiology, Ondokuz Mayis University Medical School, Samsun, Turkey. hakanomu@omu.edu.tr The aim of this study was to evaluate the antibiotic prescription rates for upper respiratory tract infections (urtis) by primary care physicians in Samsun, Turkey. Data were obtained from the records of 2,083 visits to 8 primary care areas. Trained research students were stationed on site at each of the 8 primary care areas during the study period. Clinical features of patients were documented on a standardized form. Patients who had acute pharyngitis, acute sinusitis, acute otitis media (AOM) and common cold were included in the study. This survey was conducted between June 1, 1999 and July 1, A total of 2,083 office visits were recorded and 502 (24.1%) of the patients had urtis. Physicians approached these conditions empirically, with only 2.9% of patients having a diagnostic test at initial examination. Antibiotics were prescribed for 461 patients (91.8%) with urtis (common cold: 41.9%, acute pharyngitis: 94.7%, acute sinusitis: 94.1% and AOM: 100%). 11.5% of the antibiotic prescriptions were inconsistent with current recommendations derived from the literature. Inadequate antibiotic prescribing was documented in 29.7% of antibiotic prescriptions. Errors were frequent in relation to dosage, dosage interval and duration of therapy. Overuse of antibiotics is widespread in our geographic area. Both administrative and educational intervention should be implemented to improve antibotic prescribing habits at the primary health care level to reduce the unnecessary use of antimicrobial agents. PMID: [PubMed - indexed for MEDLINE]
5 J Chemother Apr;14(2): Surveillance of antimicrobial resistance in gram-negative isolates from intensive care units in Turkey: analysis of data from the last 5 years. Leblebicioglu H, Gunaydin M, Esen S, Tuncer I, Findik D, Ural O, Saltoslu N, Yaman A, Tasova Y; Study Group. Dept of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey. hakanomu@omu.edu.tr A multicenter antimicrobial surveillance program was established in Turkey in 1995 to monitor the predominant Gram-negative pathogens from intensive care units (ICUs) and antimicrobial resistance patterns of these isolates. Sixteen hospitals participated in the study and a total of 1479 isolates from 1,100 patients were collected. The isolates were tested for their susceptibility against 13 antibiotics by E-test method. Minimum inhibitory concentrations (MICs) for each isolate were determined for imipenem, ceftazidime, ceftazidime-clavulanate, cefoperazone-sulbactam, ceftriaxone, cefepime, cefuroxime, piperacillin-tazobactam, ticarcillin-clavulanate, gentamicin, amikacin and ciprofloxacin. The most common isolates were Pseudomonas spp. (28.2%), Escherichia coli (19.2%) and Klebsiella spp. (19.1%). We found very high resistance rates to all major antibiotics that are used to treat serious infections. Although imipenem is the most active agent, it had an overall susceptibility rate of 68%. Half of the tested Klebsiella spp. strains were found to produce ESBL. This is a very high rate when compared with the literature. Cross-resistance among species was also investigated. 52% of ciprofloxacin-resistant strains were also resistant to imipenem, 80% to ceftazidime, 97% to ceftriaxone, 86% to amikacin and 19% of imipenem-resistant strains were susceptible to ceftazidime and 18% to amikacin. When susceptibilities of the years 1995 and 1999 were compared, the most interesting finding was the decrease in resistance to 3rd generation cephalosporins. In conclusion, this national clinical isolate database shows that resistance rates are high, the change over years is not predictable and continuous surveillance is necessary to monitor antimicrobial resistance and to guide antibacterial therapy. PMID: [PubMed - indexed for MEDLINE] Eur J Ophthalmol Jan-Mar;11(1):31-6. Does surgical technique influence cataract surgery contamination? Koç F, Akçam Z, Kuruoğlu S, Oge I, Günaydin M. Department of Ophthalmology, Ondokuz Mayis University, School of Medicine, Samsun, Turkey. dr_feray@yahoo.com PURPOSE: To compare cataract surgery contamination rates in large-incision extracapsular cataract extraction (ECCE) and phacoemulsification (PE), we studied
6 65 cases prospectively. METHODS: Thirty-five cases were operated by large-incision ECCE (Group I) and 30 by PE (Group II). Conjunctival swab cultures were taken immediately before surgery and anterior chamber aspirate was taken for culture upon completion of surgery for each case. RESULTS: Anterior chamber cultures were positive in 22.8% of the cases in group I and 23% in Group II. Frequencies of contamination in each group were no different (x2: 0.22, p>0.05). When the contaminations were evaluated in relation to operating time, prolongation of the operating time raised the contamination rate in Group I (p<0.05) but not in Group II (p>0.05). Silicone and PMMA intraocular lenses (IOL) were tested to see whether they had any additional risk of contamination. The frequencies of contaminated silicone IOL implanted cases (6/26) and contaminated PMMA IOL implanted cases (8/39) were similar (x2: 0.36, p>0. 05). CONCLUSIONS: Although the architecture of the incision and irrigation dynamics provided an advantage to the PE technique as the operating time became longer, routine PE was not superior to classical ECCE with respect to contamination when performed in the same circumstances. Prolonging the operating time raised the contamination rate in classical ECCE. PMID: [PubMed - indexed for MEDLINE] J Chemother Dec;12(6): A comparison of the E-test and proportion methods for susceptibility testing of Mycobacterium tuberculosis. Sanic A, Gunaydin M, Coban AY, Tokac, Cetin M. Ondokuz Mayis University Medical Faculty, Microbiology Department, Samsun, Turkey. asanic@samsun.omu.edu.tr The aim of this study was to investigate the correlation between three antibiotic susceptibility methods, the proportion method on Löwenstein Jensen medium (LJ medium), the proportion method on Middlebrook 7H11 agar (7H11 agar), and the E-test for Mycobacterium tuberculosis. Fifty M. tuberculosis isolates were tested in vitro against isoniazid, rifampicin, streptomycin, and ethambutol according to the E-test, the proportion methods on 7H11 agar and LJ medium and then compared with a reference test which was the proportion method on 7H11 agar. The correlations between proportion method on 7H11 agar and proportion method on LJ medium for isoniazid, rifampicin, streptomycin, and ethambutol were 93.9%, 85.1%, 85.4% and 78.7% respectively. The correlations between the proportion method on 7H11 agar and the E-test were 83.1%, 78.8%, 84.7% and 80.5% respectively. There were no significant differences observed between the E-test and LJ medium compared to 7H11 agar. The average times to obtain susceptibility test results were 7 and 21 days for the E-test and agar proportion methods, respectively. The E-test may be suitable for replacing the proportion methods (7H11 agar and LJ medium) in routine practice due to its fast and easy application. PMID: [PubMed - indexed for MEDLINE]
7 22. J Antimicrob Chemother Oct;46(4):649. Surveillance of antimicrobial resistance among gram-negative isolates from intensive care units in eight hospitals in turkey Aksaray Sg, Dokuzoguz B, Guvener E, Yucesoy M, Yulug N, Kocagoz S, Unal S, Cetin S, Calangu S, Gunaydin M, Leblebicioglu H, Esen S, Bayar B, Willke A, Findik D, Tuncer I I, Baysal B, Gunseren F, Mamikoglu L. Ankara Numune Hospital. Dokuz Eylul University Faculty of Medicine. Hacettepe University Faculty of Medicine. Istanbul University Faculty of Medicine. Ondokuz Mayis University Faculty of Medicine. A. U. Ibn-i Sina Hospital. Selcuk University Fa. PMID: [PubMed - as supplied by publisher] Nephron Oct;86(2):224. Do erythrocyte sedimentation rate and C-reactive protein levels have diagnostic usefulness in patients with renal failure? Arik N, Bedir A, Günaydin M, Adam B, Halefi I. PMID: [PubMed - indexed for MEDLINE] J Antimicrob Chemother May;45(5): Surveillance of antimicrobial resistance among gram-negative isolates from intensive care units in eight hospitals in Turkey. Aksaray S, DokuzoGuz B, Güvener E, Yücesoy M, Yuluğ N, Kocagöz S, Unal S, Cetin S, Calangu S, Günaydin M, Leblebicioğlu H, Esen S, Bayar B, Willke A, Findik D, Tuncer I, Baysal B, Günseren F, Mamikoğlu L. Ankara Numune Hospital. Dokuz Eylül University Faculty of Medicine. Ankara Numune Hospital, Turkey. Erratum in J Antimicrob Chemother 2000 Oct;46(4):649. With the participation of eight major reference hospitals in Turkey, 749 aerobic Gram-negative isolates obtained from 473 intensive care patients in 1997 were tested for their susceptibility to 13 commonly employed antibacterial agents. The frequency with which species were isolated and resistance rates were compared with data from the previous 2 years. Imipenem was the most active agent against the majority of isolates (75%), followed by ciprofloxacin, cefepime and amikacin. The per cent susceptibility to all antibiotics declined from 1995 to With the exception of imipenem, for which there was no change in resistance, the per cent susceptibility somewhat increased in However, it was still lower than in 1995.
8 PMID: [PubMed - indexed for MEDLINE] J Chemother Apr;11(2): Detection of extended spectrum beta-lactamases in Escherichia coli and Klebsiella pneumoniae. Leblebicioglu H, Nas Y, Eroglu C, Sunbul M, Esen S, Gunaydin M. Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University, Medical School, Samsun, Turkey. hakanomu@writeme.com The presence of extended spectrum beta-lactamases (ESbetaLs) in 129 Escherichia coli and 128 Klebsiella pneumoniae strains was determined with the E test. beta-lactamases were detected in 66 (51.2%) of E.coli and 107 (83.6%) of K. pneumoniae by the nitrocefin disk method. Putative ESbetaL production was observed in 15.5% of E. coli and 55.5% of K. pneumoniae with the E test. Among the putative ESbetaL-producer strains 11 were found to be susceptible to ceftizoxime, 6 to ceftazidime, 2 to ceftriaxone, cefotaxime and aztreonam, and one to cefoperazone according to the disk diffusion test. All putative ESbetaL-producers were resistant to cefodizime, thus it may be a good indicator of the presence of mainly TEM-type ESbetaL. The E test was also found to be practicable for the detection of ESbetaLs in clinical laboratory. Further studies are needed to clarify whether ESbetaL can be identified reliably in species that, in addition to plasmid mediated ESbetaL production, chromosomally encoded AmpC beta-lactamases and other class A ESbetaLs by disk diffusion test. PMID: [PubMed - indexed for MEDLINE] J Antimicrob Chemother Mar;43(3): A surveillance study of antimicrobial resistance of gram-negative bacteria isolated from intensive care units in eight hospitals in Turkey. Günseren F, Mamikoğlu L, Oztürk S, Yücesoy M, Biberoğlu K, Yuluğ N, Doğanay M, Sümerkan B, Kocagöz S, Unal S, Cetin S, Calangu S, Köksal I, Leblebicioğlu H, Günaydin M. Akdeniz University Faculty of Medicine, Turkey. This study was carried out with the participation of eight hospitals in Turkey to determine the frequency of gram-negative bacteria isolated in intensive care units (ICU) and to compare their resistance rates to selected antibiotics. Aerobic gram-negative bacteria isolated from ICUs during 1996 were studied. Antibiotic susceptibilities to imipenem, ceftazidime, ceftazidime-clavulanate, ceftriaxone, cefotaxime, cefepime, cefodizime, cefuroxime, piperacillin/tazobactam, amoxycillin-clavulanate, gentamicin, amikacin and ciprofloxacin were determined by Etest. A total of 748 isolates were obtained from 547 patients. The majority of organisms were isolated from the respiratory (38.8%) and urinary tracts (30.9%). Pseudomonas spp. were the most frequently
9 isolated gram-negative species (26.8%), followed by Klebsiella spp. (26.2%). Escherichia coli, Acinetobacter spp. and Enterobacter spp. were the other commonly isolated organisms. High resistance rates were observed for all antibiotics studied. Imipenem appeared to be the most active agent against the majority of isolates. Although resistance rates exceeded 50%, ciprofloxacin, cefepime and amikacin were found to be relatively effective. Extended-spectrum beta-lactamase (ESBL) production appeared to be a major mechanism of resistance to beta-lactam antibiotics. In contrast to ceftazidime-clavulanate, piperacillin/tazobactam showed poor activity against organisms thought to produce ESBL, suggesting the presence of an enzyme resistant to tazobactam action. This study has yielded high rates of resistance in aerobic gram-negative isolates from ICUs in Turkey. High resistance rates to all the other antibacterials studied leave imipenem as the only reliable agent for the empirical treatment of ICU infections in Turkey. PMID: [PubMed - indexed for MEDLINE] J Chemother Feb;11(1):46-9. Elution of vancomycin and tobramycin bonded to vascular grafts. Leblebicioglu H, Sencan I, Gunaydin M, Esen S, Eroglu C, Sunbul M. Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University, Medical School, Samsun, Turkey. hakanomu@writeme.com The elution of vancomycin and tobramycin from vascular grafts sealed with collagen and human blood was studied in vitro. The release of antibiotics was measured in three different types of soaked grafts, including grafts soaked with antibiotic after being sealed with albumin, those sealed with antibiotic and albumin mixture and those impregnated with fresh blood and antibiotic mixture. Each antibiotic was tested at two different concentrations, i.e. 5 mg/ml and 10 mg/ml for vancomycin and 2 mg/ml and 5 mg/ml for tobramycin. The eluted antibiotic concentrations were determined by the fluorescence polarization immunoassay. Initially large quantities of antibiotics were quickly eluted, depending on the amount of antibiotic mixture. A measurable amount of vancomycin was released for 3 days. There was no difference between the elution kinetics of the two antibiotics from the three different soaked grafts (p>0.05). Antibiotic-soaked grafts provided zones of inhibition against Staphylococcus aureus on Trypticase soy agar plate for up to 24 h. These results suggest that local application for 24 h of vancomycin or tobramycin with vascular grafts may be effective to prevent graft infection as shown by the fluorescence polarization immunoassay. PMID: [PubMed - indexed for MEDLINE] Nephron Aug;79(4):507. Hepatitis G and erythropoietin therapy in patients undergoing hemodialysis. Cengiz K, Günaydin M, Akpolat T, Pekbay A.
10 PMID: [PubMed - indexed for MEDLINE] Infection Sep-Oct;25(5): Prevalence of serum HGV-RNA among hemodialysis patients in Turkey. Günaydin M, Bedir A, Akpolat T, Kuku I, Pekbay A, Esen S, Ozyilkan E, Arik N, Cengiz K. Dept. of Clinical Microbiology, Ondokuz Mayis University, School of Medicine, Samsun, Turkey. A possible agent for human non-a-e hepatitis has been identified and named hepatitis G virus (HGV). The aim of this study is to evaluate the prevalence of serum HGV-RNA among hemodialysis patients in our country and the possible correlations of serum HGV-RNA with antibody to hepatitis C virus (anti-hcv), chronic liver dysfunction, number of blood transfusions, serum hepatitis B surface antigen (HBs Ag), duration of hemodialysis therapy, history of renal transplantation and patients' age and sex. Seventy-eight hemodialysis patients and 59 healthy controls were included in the study. Twenty-seven of 78 hemodialysis patients (34.6%) and two of the 59 healthy controls were serum HGV-RNA positive (p < 0.01, x2 = 17.8). There was no significant difference between the HGV-RNA positive and HGV-RNA negative groups regarding mean duration of dialysis therapy, anti-hcv, chronic liver dysfunction, number of blood transfusions, serum HBs Ag, duration of hemodialysis therapy, history of renal transplantation and patients' age and sex. In conclusion, hemodialysis patients carry the risk for HGV infection and transmission routes and clinical significance of HGV infection in these patients remain to be defined. PMID: [PubMed - indexed for MEDLINE] Ann Saudi Med Jan;17(1):86-7. Acute hepatitis A virus infection and meningoencephalitis. Leblebicioglu H, Esen S, Sunbul M, Gunaydin M. Ondokuz Mayis University Medical School, Samsun. PMID: [PubMed] Clin Microbiol Infect Mar;1(3): In-vitro release of vancomycin and netilmicin from bone cement. Leblebicioglu H, Saniç A, Günaydin M, Sencan I, Dabak N, Nas Y. Ondokuz Mayis University Medical School, Samsun, Turkey. PMID: [PubMed - as supplied by publisher]
11 Eur J Clin Microbiol Infect Dis Mar;15(3): The validity of Spanos' and Hoen's models for differential diagnosis of meningitis. Leblebicioğlu H, Esen S, Bedir A, Günaydin M, Saniç A. Comment on Eur J Clin Microbiol Infect Dis Apr;14(4): PMID: [PubMed - indexed for MEDLINE] Nephron. 1996;74(4):730. Seroprevalence of hepatitis E in hemodialysis patients in Turkey. Cengiz K, Ozyilkan E, Coşar AM, Günaydin M. PMID: [PubMed - indexed for MEDLINE] Scand J Infect Dis. 1996;28(6): Weil's disease: report of 12 cases. Leblebicioglu H, Sencan I, Sünbül M, Altintop L, Günaydin M. Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayis University Medical School, Samsun, Turkey. The epidemiological distribution and clinical features of 12 cases of Weil's disease from Turkey, are reviewed. The disease is most common in male farmers from rural areas. Myalgia and jaundice were recorded in all patients. Signs included vomiting in 9 patients, haemorrhages in 6, and renal function was impaired in 6. Creatine phosphokinase levels were found above normal limits in 75% of the cases. Leptospires were demonstrated with dark-field microscopy in the blood of 9 and in the urine of 5 of these patients. The diagnosis was confirmed with microscopic agglutination test (MAT) as well as with ELISA. Ig M antibodies were detected in 11 (92%) of the patients and is an accurate marker for acute leptospirosis. Penicillin was used for therapy and the outcome was favorable in 10 patients. Two patients died. It should be kept in mind that leptospirosis is an extremely severe disease which requires appropriate examinations at the right moment. PMID: [PubMed - indexed for MEDLINE] Ann Saudi Med Nov;15(6): Comparison of mebendazole with metronidazole and furazolidone in the treatment of
12 Giardiasis in children. Kalayci AG, Cetinkaya F, Günaydin M, Gürses N. Departments of Pediatrics and Microbiology, Ondokuz Mayis University School of Medicine, Samsun, Turkey. PMID: [PubMed] Nephrol Dial Transplant. 1995;10(4): Prevalence of anti-hcv among haemodialysis patients in Turkey: a multicentre study. Akpolat T, Arik N, Günaydin M, Utaş C, Dilek K, Caglar S, Candan F, Süleymanlar G, Paydaş S, Sen S, et al. Ondokuz Mayis University School of Medicine, Samsun, Turkey. PMID: [PubMed - indexed for MEDLINE] Infection Sep-Oct;22(5):366. Prevalence of HIV infection among hemodialysis patients in Turkey. Arik N, Akpolat T, Günaydin M, Utas C, Süleymanlar G. PMID: [PubMed - indexed for MEDLINE] Mikrobiyol Bul Oct;27(4): [Hepatitis markers in hemodialysis patients]. [Article in Turkish] Leblebicioğlu H, Günaydin M, Cengiz K, Işlek I. Ondokuz Mayis Universitesi, Tip Fakültesi Klinik Mikrobiyoloji ve Enfeksiyon Hastaliklari Anabilim Dali. In this study, antibodies against blood-borne hepatitis viruses (Hepatitis B, C and D) were investigated by ELISA in 43 patients with chronic renal insufficiency, at Hemodialysis Unit of Ondokuz Mayis University, Faculty of Medicine. 32 (76.2%) of the patients were found positive Anti-HBc total antibody and 12 (27.5%) patients were HBsAg carriers. 14 (32.5%) patients had Anti-HBs which showed protective immunity. Anti-HBs antibody was found negative in 6 patients after Hepatitis B infection. 9 (75%) of the HBsAg carriers had Anti-Delta antibody. 34 (79.1%) patients showed Anti-HCV positivity, 6 (18.2%) of HCV seropositive patients were found to be positive for HBsAg. There wasn't any correlation between length of time on hemodialysis, age and seropositivity of hepatitis markers. A correlation was detected between multiple transfusion and
13 Anti-HCV seropositivity. The prevalence of HBsAg, Anti-HBc total and Anti-HCV were sequentially found 6%, 38%, 1% among healthy blood donors taken as a control group. Hepatitis virus infection was frequently seen in hemodialysis patients because of multiple blood transfusion. Chronicity is high because of insufficiency of cellular and humoral immunity. Testing blood for hepatitis viruses markers before blood transfusion and Hepatitis B vaccination programme might control viral hepatitis infection in hemodialysis units. PMID: [PubMed - indexed for MEDLINE] Mikrobiyol Bul Apr;27(2): [Hepatitis b seroprevalence in hospital personnel]. [Article in Turkish] Leblebicioğlu H, Günaydin M, Durupinar B. Ondokuz Mayis Universitesi, Tip Fakültesi Klinik Mikrobiyoloji ve Infeksiyon Hastaliklari Anabilim Dali Yardimci Doçenti. To investigate the epidemiology of viral hepatitis B (VHB) infection in Ondokuz Mayis University Hospital staff sera of 243 workers and 100 blood donors examined for HBsAg and Anti-HBs with ELISA. The prevalences of HBsAg and Anti-HBs among the workers were 8.6% and 33.7% and among the blood donors were 5 and 24%, respectively. The overall prevalence of donors was 29%. The risk of infection showed a correlation with age and working period but there was no difference according to the jobs and departments. According to these results hospital staff have a great risk for VHB and should be vaccinated for protection from the infection. PMID: [PubMed - indexed for MEDLINE] Mikrobiyol Bul Oct;26(4): [Importance of the enzyme-linked immunosorbent assay (ELISA) for the serologic diagnosis of tuberculosis]. [Article in Turkish] Durupinar B, Yanbeyi S, Leblebicioğlu H, Günaydin M. Ondokuz Mayis Universitesi Tip Fakültesi Mikrobiyoloji Anabilim Dali. Tuberculosis is still a major granulomatosis disease in developing countries. The diagnosis of tuberculosis infection frequently creates problems which is related to clinical, radiological and bacteriological investigations. Recently, immunoenzymatic assays provide hopeful results for the serodiagnosis of tuberculosis. In this study, ELISA was evaluated as a serodiagnostic test for tuberculosis. Sera were obtained from 40 pulmonary tuberculosis patients and 15 healthy volunteer donors. The results showed that ELISA could be used in the serologic diagnosis of pulmonary tuberculosis.
14 PMID: [PubMed - indexed for MEDLINE] 48. Mikrobiyol Bul Jan;25(1): [Incidence of rubella IgG antibodies in young girls]. [Article in Turkish] Sengül AZ, Tuncer I, Günaydin M, Baykan M, Ozerol IH. Selçuk Universitesi, Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim, Dali, Konya. Rubella IgG antibodies were searched in 222 sera from female students of Selçuk University School of Medicine and from Institute of Higher Education for Nurses aged between years. Of these 31 (13.96%) were found seronegative by ELISA. We discussed the risk of infection of these young girls. PMID: [PubMed - indexed for MEDLINE] Mikrobiyol Bul. 1988;22(4): [Antibiotic-resistant microorganisms isolated from the urine of patients with suspected urinary tract infections]. [Article in Turkish] Tuncer I, Sengil AZ, Findik D, Ergun H, Günaydin M. Selçuk Universitesi, Tip Fakültesi, Mikrobiyoloji Dali Oğretim Uyesi, Ankara. In our study 5331 urine specimen have been examined. In 22 of the total 2 types of bacteria have been isolated and in the cultures of 1167 patients colony counts over than /ml. have been determined. These are 599 Escherichia coli, 161 Hemolytic Escherichia coli, 104 Klebsiella, 93 Proteus, 81 Pseudomonas, 65 Staphylococcus epidermidis, 44 Staphylococcus aureus, 24 Enterobacter, 18 Enterococcus. 602 of the infected patients were women and 565 were men. According to antibiogram results microorganisms were mostly resistant to Tetracycline (97.7%), subsequently to Ampicillin (87.5%) and to Trimethoprim-sulfamethoxazole (72.8%). Ofloxacin (97.7%), Ceftriaxone (86.4%) and Cefotaxime (86.1%) were found effective on these organisms. PMID: [PubMed - indexed for MEDLINE]
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