enix Stem Cell Research Assays

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1 HemoGenix enix Stem Cell Research Assays Methylcellulose CFU-Assays For Hematopoietic Cell Processing These are reagents and assays that allow the clonal detection in methylcellulose. Designed for hematopoietic stem- and progenitor cells they provide an indication for erythroid, neutrophil and platelet production. ColonyGro Methylcellulose CFU Master Mixes for 44 x 1ml cultures performed in duplicate CAMEO -4 Miniaturized CFU complete assay kits, including culture plates for 50 x 0.1ml cultures performed in quadruplicate ColonyGro Methylcellulose CFU Master Mixes for 44 x 1ml cultures performed in duplicate CG-0-100H ColonyGroTM without Growth Factors 100 ml CG-HPP1-100H ColonyGroTM for HPP-SP1 100 ml CG-HPP2-100H ColonyGroTM for HPP-SP2 100 ml CG-GEMM1-100H ColonyGroTM for CFC-GEMM1 100 ml CG-GEMM2-100H ColonyGroTM for CFC-GEMM2 100 ml CG-GEMM3-100H ColonyGroTM for CFC-GEMM3 100 ml CG-GEM1-100H ColonyGroTM for CFC-GEM1 100 ml CG-GEM2-100H ColonyGroTM for CFC-GEM2 100 ml CG-GEM3-100H ColonyGroTM for CFC-GEM3 100 ml CG-BFU1-100H ColonyGroTM for BFU-E1 100 ml CG-BFU2-100H ColonyGroTM for BFU-E2 100 ml CG-E-100H ColonyGroTM for CFU-E 100 ml CG-GM1-100H ColonyGroTM for GM-CFC1 100 ml CG-GM2-100H ColonyGroTM for GM-CFC2 100 ml CG-GM3-100H ColonyGroTM for GM-CFC3 100 ml CG-G-100H ColonyGroTM for G- CFC 100 ml CG-M-100H ColonyGroTM for M-CFC 100 ml CG-MK1-100H ColonyGroTM for Mk-CFC1 100 ml CG-MK2-100H ColonyGroTM for Mk-CFC2 100 ml CG-GM1-100H ColonyGroTM for GM-CFC1 100 ml CG-GM2-100H ColonyGroTM for T-CFC 100 ml CG-GM3-100H ColonyGroTM for B-CFC 100 ml

2 CAMEO -4 Miniaturized CFU complete assay kits, including culture plates for 50 x 0.1ml cultures performed in quadruplicate KC-0-100H CAMEO TM -4 without Growth Factors 38 ml + 50 plates CG-HPP1-100H CAMEO TM -4 HPP-SP1 38 ml + 50 plates CG-HPP2-100H CAMEO TM -4 HPP-SP2 38 ml + 50 plates CG-GEMM1-100H CAMEO TM -4 for CFC-GEMM1 38 ml + 50 plates CG-GEMM2-100H CAMEO TM -4 for CFC-GEMM2 38 ml + 50 plates CG-GEMM3-100H CAMEO TM -4 for CFC-GEMM3 38 ml + 50 plates CG-GEM1-100H CAMEO TM -4 for CFC-GEM1 38 ml + 50 plates CG-GEM2-100H CAMEO TM -4 for CFC-GEM2 38 ml + 50 plates CG-GEM3-100H CAMEO TM -4 for CFC-GEM3 38 ml + 50 plates CG-BFU1-100H CAMEO TM -4 for BFU-E1 38 ml + 50 plates CG-BFU2-100H CAMEO TM -4 for BFU-E2 38 ml + 50 plates CG-E-100H CAMEO TM -4 for CFU-E 38 ml + 50 plates CG-GM1-100H CAMEO TM -4 for GM-CFC1 38 ml + 50 plates CG-GM2-100H CAMEO TM -4 for GM-CFC2 38 ml + 50 plates CG-GM3-100H CAMEO TM -4 for GM-CFC3 38 ml + 50 plates CG-G-100H CAMEO TM -4 for G- CFC 38 ml + 50 plates CG-M-100H CAMEO TM -4 for M-CFC 38 ml + 50 plates CG-MK1-100H CAMEO TM -4 for Mk-CFC1 38 ml + 50 plates CG-MK2-100H CAMEO TM -4 for Mk-CFC2 38 ml + 50 plates CG-GM1-100H CAMEO TM -4 for GM-CFC1 38 ml + 50 plates CG-GM2-100H CAMEO TM -4 for T-CFC 38 ml + 50 plates CG-GM3-100H CAMEO TM -4 for B-CFC 38 ml + 50 plates

3 INSTRUMENT-BASED PROLIFERATION ASSAYS HemoGenix assays allow you to measure proliferation and differentiation of various stem- and progenitor cells. Choose from a variety of readouts including: BIOLUMINESCENCE FLORESCENCE ABSORBANCE BIOLUMINOMICS TM PRINZIPLE Luciferase ATP + Luciferin + O > Oxyluciferin + AMP + PPi + CO2 + LIGHT Mg 2+ INSTRUMENT BASED 96-well PROLIFERATION ASSAYS HALO -96 Research ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls K2-0-1H HALO -96 Research ATP bioluminescence kit 1 Plate Kit K2-HPP1-1H HALO -96 Research ATP bioluminescence kit for HPP-SP1 1 Plate Kit K2-HPP2-1H HALO -96 Research ATP bioluminescence kit for HPP-SP2 1 Plate Kit K2-GEMM1-1H HALO -96 Research ATP bioluminescence kit for CFC-GEMM1 1 Plate Kit K2-GEMM2-1H HALO -96 Research ATP bioluminescence kit for CFC-GEMM2 1 Plate Kit K2-GEM1-1H HALO -96 Research ATP bioluminescence kit for CFC-GEM1 1 Plate Kit K2-GEM2-1H HALO -96 Research ATP bioluminescence kit for CFC-GEM2 1 Plate Kit

4 K2-BFU1-1H HALO -96 Research ATP bioluminescence kit for BFU-E1 1 Plate Kit K2-BFU2-1H HALO -96 Research ATP bioluminescence kit for BFU-E2 1 Plate Kit K2-GM1-1H HALO -96 Research ATP bioluminescence kit for GM-CFC1 1 Plate Kit K2-GM3-1H HALO -96 Research ATP bioluminescence kit for GM-CFC3 1 Plate Kit K2-G-1H HALO -96 Research ATP bioluminescence kit for G- CFC 1 Plate Kit K2-MK1-1H HALO -96 Research ATP bioluminescence kit for Mk-CFC1 1 Plate Kit K2-T-1H HALO -96 Research ATP bioluminescence kit for T-CFC 1 Plate Kit K2-B-1H HALO -96 Research ATP bioluminescence kit for B-CFC 1 Plate Kit Comparison between Human Tissues in the Response of 7 Cell Population using HALO -96 HemoFLOUR -96 Fluorescence stem and progenitor cell proliferation assays K5-0-1H HemoFlour -96 Research fluorescence without Growth 1 Plate Kit Factors K5-HPP1-1H HemoFlour -96 Research fluorescence assay for HPP-SP1 1 Plate Kit K5-HPP2-1H HemoFlour -96 Research fluorescence assay for HPP-SP2 1 Plate Kit K5-GEMM1-1H HemoFlour -96 Research fluorescence assay for CFC-GEMM1 1 Plate Kit K5-GEMM2-1H HemoFlour -96 Research fluorescence assay for CFC-GEMM2 1 Plate Kit K5-GEM1-1H HemoFlour -96 Research fluorescence assay for CFC-GEM1 1 Plate Kit K5-GEM2-1H HemoFlour -96 Research fluorescence assay for CFC-GEM2 1 Plate Kit K5-BFU1-1H HemoFlour -96 Research fluorescence assay for BFU-E1 1 Plate Kit

5 K5-BFU2-1H HemoFlour -96 Research fluorescence assay for BFU-E2 1 Plate Kit K5-GM1-1H HemoFlour -96 Research fluorescence assay for GM-CFC1 1 Plate Kit K5-GM3-1H HemoFlour -96 Research fluorescence assay for GM-CFC3 1 Plate Kit K5-G-1H HemoFlour -96 Research fluorescence assay for G- CFC 1 Plate Kit K5-MK1-1H HemoFlour -96 Research fluorescence assay for Mk-CFC1 1 Plate Kit K5-T-1H HemoFlour -96 Research fluorescence assay for T-CFC 1 Plate Kit K5-B-1H HemoFlour -96 Research fluorescence assay for B-CFC 1 Plate Kit HemoLIGHT -96 Absorbance / colorimetric stem and progenitor cell assays K4-0-1H HemoLIGHT TM -96 Research absorbance without Growth Factors 1 Plate Kit KCO-HPP1-1H HemoFlour -96 Research absorbance assay for HPP-SP1 1 Plate Kit KCO-HPP2-1H HemoFlour -96 Research absorbance assay for HPP-SP2 1 Plate Kit KCO-GEMM1-1H HemoFlour -96 Research absorbance assay for CFC-GEMM1 1 Plate Kit KCO-GEMM2-1H HemoFlour -96 Research absorbance assay for CFC-GEMM2 1 Plate Kit KCO-GEM1-1H HemoFlour -96 Research absorbance assay for CFC-GEM1 1 Plate Kit KCO-GEM2-1H HemoFlour -96 Research absorbance assay for CFC-GEM2 1 Plate Kit KCO-BFU1-1H HemoFlour -96 Research absorbance assay for BFU-E1 1 Plate Kit KCO-BFU2-1H HemoFlour -96 Research absorbance assay for BFU-E2 1 Plate Kit KCO-GM1-1H HemoFlour -96 Research absorbance assay for GM-CFC1 1 Plate Kit KCO-GM3-1H HemoFlour -96 Research absorbance assay for GM-CFC3 1 Plate Kit KCO-G-1H HemoFlour -96 Research absorbance assay for G- CFC 1 Plate Kit KCO-MK1-1H HemoFlour -96 Research absorbance assay for Mk-CFC1 1 Plate Kit KCO-T-1H HemoFlour -96 Research absorbance assay for T-CFC 1 Plate Kit CG-B-1H HemoFlour -96 Research absorbance assay for B-CFC 1 Plate Kit CAMEO -96 (combined CFU & Bioluminescence) Methylcellulose assay for manual colony enumeration AND standardized ATP-bioluminescence KCO-0-1H CAMEO TM -96 without Growth Factors 1 Plate Kit KCO-HPP1-1H CAMEO TM -96 HPP-SP1 1 Plate Kit KCO-HPP2-1H CAMEO TM -96 HPP-SP2 1 Plate Kit KCO-GEMM1-1H CAMEO TM -96 for CFC-GEMM1 1 Plate Kit KCO-GEMM2-1H CAMEO TM -96 for CFC-GEMM2 1 Plate Kit KCO-GEM1-1H CAMEO TM -96 for CFC-GEM1 1 Plate Kit

6 KCO-GEM2-1H CAMEO TM -96 for CFC-GEM2 1 Plate Kit KCO-BFU1-1H CAMEO TM -96 for BFU-E1 1 Plate Kit KCO-BFU2-1H CAMEO TM -96 for BFU-E2 1 Plate Kit KCO-GM1-1H CAMEO TM -96 for GM-CFC1 1 Plate Kit KCO-GM3-1H CAMEO TM -96 for GM-CFC3 1 Plate Kit KCO-G-1H CAMEO TM -96 for G- CFC 1 Plate Kit KCO-MK1-1H CAMEO TM -96 for Mk-CFC1 1 Plate Kit KCO-T-1H CAMEO TM -96 for T-CFC 1 Plate Kit CG-B-1H CAMEO TM -96 for B-CFC 1 Plate Kit SUSPENSION EXPANSION CULTURE (SEC) MASTER MIXES CellExpand TM Expansion of hematopoetic stem and progenitor cells SEC-0-15H SEC-0-40H SEC-HPP1-15H SEC-HPP1-40H SEC-HPP2-15H SEC-HPP2-40H SEC-GEMM1-15H SEC-GEMM1-40H SEC-GEMM2-15H SEC-GEMM2-40H SEC-GEMM3-15H SEC-GEMM3-40H SEC-GEM1-15H SEC-GEM1-40H SEC-GEM2-15H SEC-GEM2-40H SEC-BFU1-15H SEC-BFU1-40H SEC-BFU2-15H SEC-BFU2-40H SEC-GM1-15H SEC-GM1-40H SEC-GM3-15H SEC-GM3-40H SEC-Mk1-15H SEC-Mk1-40H SEC-T-15H SEC-T-40H SEC-B-15H SEC-B-40H CellExpand TM : master mix without growth factors CellExpand TM : master mix for HPP-SP1 CellExpand TM : master mix for HPP-SP2 CellExpand TM : master mix for CFC-GEMM1 CellExpand TM : master mix for CFC-GEMM2 CellExpand TM : master mix for CFC-GEMM3 CellExpand TM : master mix for CFC-GEM1 CellExpand TM : master mix for CFC-GEM2 CellExpand TM : master mix for BFU-E1 CellExpand TM : master mix for BFU-E2 CellExpand TM : master mix for GM-CFC1 CellExpand TM : master mix for GM-CFC3 CellExpand TM : master mix for MK-CFC1 CellExpand TM : master mix for T-CFC CellExpand TM : master mix for B-CFC

7 IMMUNE CELL FUNCTIONAL ASSAYS Whether it is a simple lymphocyte proliferation assay, donor lymphocyte infusion (DLI) testing or mixed lymphocyte cultures, these assays are flexible for any laboratory ImmunoGlo -96 ATP bioluminescence lymphocyte proliferation assay Uses of ImmunoGlo TM -96 Lymphocyte activation Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc Cellular immune response studies Single-cell, T-lymphocyte cloning studies Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use Effect of accessory (non T-cells) on T cell induction Effect of co-stimulators on T-cell induction KM1-LPA-1 ImmunoGlo -96 bioluminescence lymphocyte proliferation assay 1 Plate Kit KM1-LPA-2 ImmunoGlo -96 bioluminescence lymphocyte proliferation assay 2 Plate Kit KM1-LPA-4 ImmunoGlo -96 bioluminescence lymphocyte proliferation assay 4 Plate Kit ImmunoGlo -MLC ATP bioluminescence 1- or 2-way mixed lymphocyte culture assay Uses of ImmunoGlo -MLC One-way mixed lymphocyte culture (MLC) / reaction (MLR) Two-way mixed lymphocyte culture (MLC) / reaction (MLR) Allogeneic mixed lymphocyte culture. Cellular immune response Dendritic cell interactions Mesenchymal stem/stromal cell interactions Investigations of agents that activate/suppress MLC/MLR KM1-MLC-1 ImmunoGlo -96 MLC bioluminescence lymphocyte proliferation assay 1 Plate Kit KM1-MLC-2 ImmunoGlo -96 MLC bioluminescence lymphocyte proliferation assay 2 Plate Kit KM1-MLC-4 ImmunoGlo -96 MLC bioluminescence lymphocyte proliferation assay 4 Plate Kit

8 ImmunoFLUOR -96 Fluorescence lymphocyte proliferation assay Uses of ImmunoFluor TM -96 Lymphocyte activation Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc Cellular immune response studies Single-cell, T-lymphocyte cloning studies Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use Effect of accessory (non T-cells) on T cell induction Effect of co-stimulators on T-cell induction KM2-LPA-1 ImmunoFLUOR -96 fluorescence lymphocyte proliferation assay 1 Plate Kit KM2-LPA-2 ImmunoFLUOR -96 fluorescence lymphocyte proliferation assay 2 Plate Kit KM2-LPA-4 ImmunoFLUOR -96 fluorescence lymphocyte proliferation assay 4 Plate Kit ImmunoFLUOR -MLC Fluorescence 1- or 2-way mixed lymphocyte culture assay Uses of ImmunoFluor -MLC One-way mixed lymphocyte culture (MLC) / reaction (MLR) Two-way mixed lymphocyte culture (MLC) / reaction (MLR) Allogeneic mixed lymphocyte culture. Cellular immune response Dendritic cell interactions Mesenchymal stem/stromal cell interactions Investigations of agents that activate/suppress MLC/MLR KM2-MLC-1 ImmunoFLUOR -MLC 96-well fluorescence MLC assay 1 Plate Kit KM2-MLC-2 ImmunoFLUOR -MLC 96-well fluorescence MLC assay 2 Plate Kit KM2-MLC-4 ImmunoFLUOR -MLC 96-well fluorescence MLC assay 4 Plate Kit

9 ImmunoLIGHT -96 Absorbance / colorimetric lymphocyte proliferation assay Uses of ImmunoFluor TM -96 Lymphocyte activation Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc Cellular immune response studies Single-cell, T-lymphocyte cloning studies Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use. Effect of accessory (non T-cells) on T cell induction. Effect of co-stimulators on T-cell induction. KM3-LPA-1 ImmunoLIGHT -96 absorbance lymphocyte proliferation assay 1 Plate Kit KM3-LPA-2 ImmunoLIGHT -96 absorbance lymphocyte proliferation assay 2 Plate Kit KM3-LPA-4 ImmunoLIGHT -96 absorbance lymphocyte proliferation assay 4 Plate Kit ImmunoLIGHT -MLC Absorbance / colorimetric mixed lymphocyte culture assay Uses of ImmunoFluor -MLC One-way mixed lymphocyte culture (MLC) / reaction (MLR) Two-way mixed lymphocyte culture (MLC) / reaction (MLR) Allogeneic mixed lymphocyte culture. Cellular immune response Dendritic cell interactions Mesenchymal stem/stromal cell interactions Investigations of agents that activate/suppress MLC/MLR KM3-MLC-1 ImmunoLIGHT -MLC 96-well fluorescence MLC assay 1 Plate Kit KM3-MLC-2 ImmunoLIGHT -MLC 96-well fluorescence MLC assay 2 Plate Kit KM3-MLC-4 ImmunoLIGHT -MLC 96-well fluorescence MLC assay 4 Plate Kit

10 MESENCHYMAL STEM/STROMAL CELL (MSC) ASSAYS AND REAGENTS Use a quantitative MSC proliferation assay to characterize an MSC-derived products Monitor small, medium and large (bulk) MSC expansion (passaging) The MSCGlo TM -96 Research includes standards and controls to calibrate and standardize For replacement of the manual CFU-F assays. Multiplex with other assays such as flow cytometry for MSC and differentiation markers and gene expression analysis to obtain and correlate information from a single sample MSCGlo -96 Research ATP bioluminescence MSC proliferation assay KLMC-LS-1 MSCGlo TM -96 bioluminescence proliferation assay, low serum 1 Plate Kit KLMC-SF-1 MSCGlo TM -96 bioluminescence proliferation assay, serum free 1 Plate Kit KLMC-HM-1 MSCGlo TM -96 bioluminescence proliferation assay, humanized 1 Plate Kit KLMC-HSF-1 MSCGlo TM -96 bioluminescence proliferation assay, humanized, serum free 1 Plate Kit The assays also available for dog and horse MSCs MSCFluor -96 Research Fluorescence MSC proliferation assay KMCF-LS-1 MSCFlour TM -96 bioluminescence proliferation assay, low serum 1 Plate Kit KMCF-SF-1 MSCFlour TM -96 bioluminescence proliferation assay, serum free 1 Plate Kit KMCF-HM-1 MSCFlour TM -96 bioluminescence proliferation assay, humanized 1 Plate Kit KMCF-HSF-1 MSCFlour TM -96 bioluminescence proliferation assay, humanized, serum free 1 Plate Kit The assays also available for dog and horse MSCs MSCLight -96 Research Absorbance / colorimetric MSC proliferation assay KMCL-LS-1 MSCLight TM -96 bioluminescence proliferation assay, low serum 1 Plate Kit KMCL-SF-1 MSCLight TM -96 bioluminescence proliferation assay, serum free 1 Plate Kit KMCL-HM-1 MSCLight TM -96 bioluminescence proliferation assay, humanized 1 Plate Kit KMCL-HSF-1 MSCLight TM -96 bioluminescence proliferation assay, humanized, serum free 1 Plate Kit The assays also available for dog and horse MSCs

11 STEMAssays - PRIMARY RY STEM CELLS; STEM CELL LINES STEMGlo -96 Research Standardized ATP-bioluminescence for adherent primary cells, ES, ips and cell lines KSG-96-A-1 STEMGlo TM -96 Research for adherent stem cells, ES, ips, cell lines 1 Plate Kit KSG-96-NA-1 STEMGlo TM -96 Research for non- adherent stem cells, ES, ips, cell lines 1 Plate Kit STEMFluor -96 Research Fluorescence proliferation assay for adherent primary cells, ES, ips and cell lines KSF-96-A-1 STEMFlour TM -96 Research for adherent stem cells, ES, ips, cell lines 1 Plate Kit KSF-96-NA-1 STEMFlour TM -96 Research for adherent stem cells, ES, ips, cell lines 1 Plate Kit STEMLight -96 Research Absorbance proliferation assays for adherent primary cells, ES, ips and cell lines KSL-96-A-1 STEMLight TM -96 Research for adherent stem cells, ES, ips, cell lines 1 Plate Kit KSL-96-NA-1 STEMLight TM -96 Research for adherent stem cells, ES, ips, cell lines 1 Plate Kit STEMClone Clonal Methylcellulose Proliferation and Differentiation Assay Manual Colony Enumeration AND Standardized ATP Bioluminescence Equivalent to CAMEO TM -96 Based on the classic colony-forming cell (CFC)-assay KSC well Clonal Methylcellulose Proliferation and Differentiation Assay 1 Plate Kit KSC well Clonal Methylcellulose Proliferation and Differentiation Assay 2 Plate Kit KSC well Clonal Methylcellulose Proliferation and Differentiation Assay 4 Plate Kit HepatoGlo -Tox HT Hepatotoxicity ATP-bioluminescent assay HepatoGloTM-Tox HT can incorporate fresh or frozen primary or ips-derived hepatocytes HepatoGro utilizes specialized growth medium KHG-96-A-2 HepatoGloTM-Tox HT adherent 96-well hepatotoxicity assay 2 Plate Kit KHG-96-NA-2 HepatoGloTM-Tox HT non-adherent 96-well hepatotoxicity assay KHG-384-A-2 HepatoGloTM-Tox HT adherent 384-well hepatotoxicity assay KHG-384-NA-2 HepatoGloTM-Tox HT non-adherent 384-well hepatotoxicity assay 2 Plate Kit 2 Plate Kit 2 Plate Kit

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