Preparation of cord blood for infusion: bedside thaw, dilute and wash, or somewhere in between

Size: px
Start display at page:

Download "Preparation of cord blood for infusion: bedside thaw, dilute and wash, or somewhere in between"

Transcription

1 Preparation of cord blood for infusion: bedside thaw, dilute and wash, or somewhere in between Donna Wall, MD Director, Manitoba Blood and Marrow Transplant Program ISCT 2012

2 Disclosures: none

3 The problem: Cord blood grafts contain 1/10 th the number of hematopoietic progenitors (TNC, CD34, CFU) compared to other HSPC sources and are all previously frozen. Progenitor cell loss at time of thaw must be minimized.

4 Impact on graft characteristics on engraftment Duke retrospective review of 435 primarily pediatric (median age: 5.3 years) patients receiving a single-unit unrelated UCBT after myeloablative conditioning from 2000 to 2008 Cumulative incidences of neutrophil and platelet engraftment were 76.9% and 55% respectively Page BBMT 2011

5 Precryopreservation graft characteristics on the probability of platelet engraftment. Page BBMT 2011

6 Post-thaw graft characteristics and the probability of platelet engraftment Page et al. BBMT 2011

7 Higher CFU dosing was the only precryopreservation graft characteristic predictive of neutrophil (P =.0024) and platelet engraftment (P =.0063). In the post-thaw model, CFU dose best predicted neutrophil and platelet engraftment (both P <.0001). Page et al. BBMT 2011 Yoo et al. BMT 2007

8 Objectives: To review the 3 main approaches to CBU thaw and compare transplant outcomes To highlight special issues in the red cell replete units and pediatrics

9 Historical perspective: In the early days products were frozen without red cell depletion in bags that had a high propensity to break That led to thawing in the laboratory with a wash stem to remove red cell lysate and DMSO. Product volumes were ml

10 The Rubinstein method Rationale was that during the freeze process cells became dehydrated from the hyperosmolar DMSO solution. Thus by thawing with a hyperosmolar combination of albumin and dextran one allowed cells to equilibrate more gently improving survival. The osmotic damage inflicted by the severe solute concentration gradient, however, can be averted by a simple 2-fold dilution after thawing, providing almost total recovery of viable hematopoietic progenitor cells. Rubinstein PNAS 1995

11 Products evolved Red cell and plasma depletion product volume down to 25 ml with 5 ml of DMSO Improved cryopreservation bag integrity Smaller products, often with 2 compartments

12 Bedside Thaw Adult transplant centers looked at small product (small red cell lysate and DMSO content) and concern of cell loss with wash Greatest risk was poor or non- engraftment This looks like our autologous PBSC products Hahn et al. BMT, 2003 Chow et al. BBMT 2007 Stiff et al. Blood 2005

13 Alternate approach: Dilute only Simple dilution with albumin/dextran would support laboratory thaw and potential benefit of stabilization with hyperosmolar solution 2-5 fold dilution in laboratory with 25% albumin/40% dextran solution Stability of product for at least 8 hours facilitating remote lab thaw Barker et al, BBMT 2007

14 Thaw approach Pro Con Bedside thaw Dilute only Dilute and wash No lab issues Similar to practice for other frozen products Controlled thaw Theoretical advantage of hyperosmolar reequilibration Removal of 80% of DMSO and red cell lysate Manipulation of small product at bedside Immediate infusion necessary High load of DMSO and red cell/white cell lysate Larger volume of infusion Same load of red cells and DMSO Risk of cell loss with centrifugation (bag breaking, Cell aggregation with centrifugation

15 Comparison of the 3 thaw methods St. Louis Cord Blood Bank thawed 10 products in a 37 degree water bath and immediately split product into 3 groups: Thaw only 2 fold dilution with dextran-albumin 2 fold dilution with dextran-albumin followed by centrifugation at 400g for 20 min.

16 Viable CD34 recovery between thaw methods (n = 10 per group) Dilution Wash Thaw p< 0.01 Regan et al, Transfusion 2010

17 CFC mean percent recovery (n = 10 per group), Dilute/Wash Thaw P< 0.01 Regan et al, Transfusion 2010

18 Transplant outcomes of 401 cord blood units exported from St. Louis Cord Blood Bank between All units were red cell and plasma depleted All used in single cord transplants

19 Patient characteristics Thaw N = 36 Dilute N = 163 Wash N = 202 P value Age (median) <0.01 Weight (kg) <0.01 TNC recovery 99% 78% 79% <0.01 TNC /kg <0.01 Post thaw viability <0.1 Regan et al, Transfusion 2010

20 No difference in time to neutrophil recovery Regan et al, Transfusion 2010

21 Infusional toxicity reported to the SLCBB ( ) No serious adverse events reported

22 Cell processing laboratory The cell product is much smaller than the lab is used to working with. Important to practice thaws Minimize cell loss Contingency plan to handle broken bags Careful with testing sample volumes Validated thaw procedure for the variety of products being used. Requires early communication between transplant team and laboratory

23 Pediatric considerations: DMSO load: <1 gram DMSO/kg Red cell lysate: <1 ml of red cell lysate/kg Volume: although young children can handle volume better than older adults, in general want to keep volume <20 ml/kg.

24 The red cell replete unit The products are plasma depleted resulting in hematocrit in 80% range. At thaw there is a lot of red cell and neutrophil lysate Reports of cardiac and renal toxicity when high cell numbers infused (double cord, small recipient) 120 pediatric ( y) transplants with 40% washed and 60% thaw only OS was 79% and DFS was 70 % at 3 years Petz et al. Transfusion on line 2011

25 Conclusions: For cord blood units that have been banked after red cell and volume reduction there are similar transplant outcomes with the 3 thaw approaches Special consideration is needed for pediatric recipients and red cell replete units Retrospective review of CIBMTR experience is ongoing (Ballen et al)

26 Acknowledgements: Donna Regan and Jonathan Woffard St. Louis Cord Blood Bank

27

28 Improved hematopoietic progenitor cell reservoir following cord blood vs. bone marrow transplant 2 groups of children: 12 CB recipients and 12 adult BM recipients Recipients of BM transplants received 10-fold more cells and had significantly faster neutrophil and platelet recovery At 1 year after transplantation, the frequency of colonyforming cells (CFCs) and long-term culture initiating cells (LTC-ICs) were compared Frassoni et al, Blood 102:1138

29 Comparison of marrow hematopoietic progenitors following CB vs BM transplant Cord Blood Recipients (n=12) Bone Marrow Recipients (n=12) CFC/2 x 10 4 BM mononuclear cells P =.007 LTC-IC/10 6 BM mononuclear cells P =.001

30 Public cord blood banking Despite heavy support by national bodies the cost of units is high $25-40,000/unit Chance of finding a donor increases if you collect from families of similar ethnic background Hema-Quebec is the largest public bank in Canada Smaller banks in Toronto and Edmonton

31

Cord Blood: that other stem cell source. Donna Wall, MD Director, Manitoba Blood and Marrow Transplant Program

Cord Blood: that other stem cell source. Donna Wall, MD Director, Manitoba Blood and Marrow Transplant Program Cord Blood: that other stem cell source Donna Wall, MD Director, Manitoba Blood and Marrow Transplant Program CBMTG April 2012 The problem: In order to perform a BMT from one person to another one needs

More information

Beyond Cell Dose: Selection of the Optimal Umbilical Cord Blood Unit. Karen Ballen, MD Massachusetts General Hospital June, 2012

Beyond Cell Dose: Selection of the Optimal Umbilical Cord Blood Unit. Karen Ballen, MD Massachusetts General Hospital June, 2012 Beyond Cell Dose: Selection of the Optimal Umbilical Cord Blood Unit Karen Ballen, MD Massachusetts General Hospital June, 2012 OUTLINE Cell Dose HLA Typing HLA C and KIR HLA Antibodies ABO and Racial/Ethnic

More information

Recommendations to Transplant Centres Performing Cord Blood Transplants. Why Choosing the Right Thaw Method Could Save a Patient s Life

Recommendations to Transplant Centres Performing Cord Blood Transplants. Why Choosing the Right Thaw Method Could Save a Patient s Life Recommendations to Transplant Centres Performing Cord Blood Transplants Vicki Antonenas Why Choosing the Right Thaw Method Could Save a Patient s Life Lynn O Donnell (USA) 1 The process of receiving, testing

More information

Cord Blood: Thawing & Infusion Practical Methods

Cord Blood: Thawing & Infusion Practical Methods Cord Blood: Thawing & Infusion Practical Methods Donna M Regan, MT(ASCP)SBB St. Louis Cord Blood Bank @ SSM Cardinal Glennon Children s s Medical Center Objectives Present variety of product configurations

More information

Processing & Utilization of Cord Blood for Transplant

Processing & Utilization of Cord Blood for Transplant Processing & Utilization of Cord Blood for Transplant 2010-Jan Jan-15 Nicole L. Prokopishyn, PhD HPC Processing Laboratory Director Calgary Laboratory Services Overview Cord Blood Processing Pre-Freeze

More information

Public Cord Blood Banking at the National Cord Blood Program (NCBP)

Public Cord Blood Banking at the National Cord Blood Program (NCBP) Public Cord Blood Banking at the National Cord Blood Program (NCBP) A. Scaradavou, MD Medical Director, National Cord Blood Program, New York Blood Center Associate Attending, Pediatric BMT Memorial Sloan-Kettering

More information

Pr Eliane Gluckman, MD, FRCP, Disclosure of Interest: Nothing to Disclose

Pr Eliane Gluckman, MD, FRCP, Disclosure of Interest: Nothing to Disclose Pr Eliane Gluckman, MD, FRCP, Hospital Saint Louis, University Paris- Diderot, France Should Haplo-identical transplantation be preferred to cord blood in patients without a matched donor? Disclosure of

More information

Selection of the Optimal Umbilical Cord Blood Unit

Selection of the Optimal Umbilical Cord Blood Unit Karen Ballen, MD Selection of the Optimal Umbilical Cord Blood Unit Massachusetts General Hospital September, 2013 OUTLINE Cell Dose HLA Match Allele Level HLA C KIR Directional Mismatch NIMA HLA Antibodies

More information

Umbilical Cord Blood Transplantation

Umbilical Cord Blood Transplantation Umbilical Cord Blood Transplantation V Rocha MD, PhD Hopital Saint Louis, Paris University 7 CIBMTR Milwaukee Umbilical Cord blood transplantation Background History Clinical results in children and adults

More information

Preamble. 1. If it is necessary, are you willing to receive follow up questions to help clarify any answers to the survey questions?

Preamble. 1. If it is necessary, are you willing to receive follow up questions to help clarify any answers to the survey questions? Preamble The purpose of this inter organizational survey is to gather data regarding transplant center practices for preparing cord blood units for administration, including characterizing and confirming

More information

Skeptical UCB Customer: Experiences of University of Rochester/Strong Memorial Transplant Program with Umbilical Cord Blood Units

Skeptical UCB Customer: Experiences of University of Rochester/Strong Memorial Transplant Program with Umbilical Cord Blood Units Skeptical UCB Customer: Experiences of University of Rochester/Strong Memorial Transplant Program with Umbilical Cord Blood Units Craig Mullen, MD, PhD Chief, Pediatric Hematology/Oncology/HSC Transplantation

More information

Cord Blood for Cellular Therapy: A Snapshot of this Evolving Market Landscape

Cord Blood for Cellular Therapy: A Snapshot of this Evolving Market Landscape GENReports: Market & Tech Analysis Cord Blood for Cellular Therapy: A Snapshot of this Evolving Market Landscape > Enal Razvi, Ph.D. Biotechnology Analyst, Managing Director SELECTBIO US enal@selectbio.us

More information

14.0 Stem Cell Laboratory Services

14.0 Stem Cell Laboratory Services Laboratory Services Contact Information: To inquire about assisting with surgical harvesting of bone marrow, cellular therapy (CT) product processing, cryopreservation, storage, or any other lab services,

More information

UMBILICAL CORD BLOOD TRANSPLANTATION: KFSH EXPERIENCE

UMBILICAL CORD BLOOD TRANSPLANTATION: KFSH EXPERIENCE UMBILICAL CORD BLOOD TRANSPLANTATION: KFSH EXPERIENCE HIND AL HUMAIDAN, MD,FRCPA Director, Blood Bank (Donor & Transfusion Services) and Stem Cell Cord Blood Bank Consultant Hematopathologist INTRODUCTION

More information

Umbilical cord blood (UCB) is increasingly

Umbilical cord blood (UCB) is increasingly ORIGINAL RESEARCH Comparison of cord blood thawing methods on cell recovery, potency, and infusion_2803 1..6 Donna M. Regan, Jonathan D. Wofford, and Donna A. Wall BACKGROUND: Umbilical cord blood (UCB)

More information

Pluripotent: Can differentiate into blood cells of all three lineages (erythroid, myeloid, and megakaryoblastic) Express CD 34+ antigen CD34 most

Pluripotent: Can differentiate into blood cells of all three lineages (erythroid, myeloid, and megakaryoblastic) Express CD 34+ antigen CD34 most Shan Yuan, MD May 11, 2011 Pluripotent: Can differentiate into blood cells of all three lineages (erythroid, myeloid, and megakaryoblastic) Express CD 34+ antigen CD34 most likely an adhesion molecule

More information

CPT Codes for Bone Marrow Transplant January 2015 James L. Gajewski, MD

CPT Codes for Bone Marrow Transplant January 2015 James L. Gajewski, MD The blood and marrow transplant field has 15 dedicated CPT codes. These CPT codes can be categorized into three groups: 1. Collection Codes 2. Cell Processing Codes 3. Cell Infusion Codes Collection Codes

More information

The donor search: the best donor or cord blood unit

The donor search: the best donor or cord blood unit The donor search: the best donor or cord blood unit Dr Bronwen Shaw Consultant in haematopoietic cell transplantation Royal Marsden Hospital /Anthony Nolan Overview Where do we find donors/units for transplantation

More information

Automated Cord Blood Processing:

Automated Cord Blood Processing: TM Automated Cord Blood Processing: A Follow-up to the Pilar Solves Study Comparing the AXP vs the Sepax Cord Blood Processing Systems CHRISTY KIM, PETRA CRAVENS, MINDY WILKE-DOUGLAS AND MITCHEL SIVILOTTI

More information

Navelstrengbloed tegen kanker

Navelstrengbloed tegen kanker Navelstrengbloed tegen kanker THERAPIEDAG 2008: "Zorgtrajecten in kanker" Zaterdag 27 september 2008 Gasthuisberg, Leuven. Hélène Schoemans, MD KUL, Stem Cell Institute Leuven Cord blood Collection and

More information

A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences

A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences Cytotherapy (2001) Vol. 3, No. 6, 429 433 A simple and reliable procedure for cord blood banking, processing, and freezing: St Louis and Ohio Cord Blood Bank experiences JM Alonso III 1, DM Regan 1, CE

More information

The Power & Potential of Cord Blood

The Power & Potential of Cord Blood The Power & Potential of Cord Blood Donna Regan, MT(ASCP)SBB, Director St. Louis Cord Blood Bank & Cellular Therapy Laboratory SSM Cardinal Glennon Children s Medical Center April 30, 2014 Objectives Since

More information

AUTOMATION OF THE COLONY-FORMING CELL ASSAY FOR MEASURING HEMATOPOIETIC PROGENITORS IN CORD BLOOD

AUTOMATION OF THE COLONY-FORMING CELL ASSAY FOR MEASURING HEMATOPOIETIC PROGENITORS IN CORD BLOOD AUTOMATION OF THE COLONY-FORMING CELL ASSAY FOR MEASURING HEMATOPOIETIC PROGENITORS IN CORD BLOOD Bert Wognum Oliver Egeler Caren Grande Ning Yuan Steven Woodside Terry Thomas Allen Eaves STEMCELL Technologies

More information

Cord Blood Market Trends, circa 2014

Cord Blood Market Trends, circa 2014 GENReports: Market & Tech Analysis Cord Blood Market Trends, circa 2014 > Enal Razvi, Ph.D. Managing Director Select Biosciences, Inc. enal@selectbio.us Topic Introduction and Scope The focus of this GEN

More information

Guide to the New CPT Codes for Hematopoietic Stem Cell Transplant and Apheresis

Guide to the New CPT Codes for Hematopoietic Stem Cell Transplant and Apheresis Guide to the New CPT Codes for Hematopoietic Stem Cell Transplant and Apheresis A major overhaul of the Current Procedural Terminology (CPT) codes for hematopoietic stem cell collection and transplantation

More information

The Power & Potential of Cord Blood

The Power & Potential of Cord Blood The Power & Potential of Cord Blood Donna Regan, MT(ASCP)SBB, Director St. Louis Cord Blood Bank & Cellular Therapy Laboratory SSM Cardinal Glennon Children s Medical Center September 18, 2013 Objectives

More information

Guidance for Industry and FDA Staff

Guidance for Industry and FDA Staff Guidance for Industry and FDA Staff Investigational New Drug Applications for Minimally Manipulated, Unrelated Allogeneic Placental/Umbilical Cord Blood Intended for Hematopoietic and Immunologic Reconstitution

More information

Comparability of Cord Blood Units

Comparability of Cord Blood Units Comparability of Cord Blood Units John D. McMannis,, Ph.D. Professor of Cancer Medicine Director, Cell Therapy Laboratory Technical Director, UT MDAnderson Cord Blood Bank Liaison Meeting, Bethesda June

More information

Pros and Cons of Stem Cell Sources and their availability in Africa. Dr Jaimendra Singh Inkosi Albert Luthuli Central Hospital Durban, South Africa

Pros and Cons of Stem Cell Sources and their availability in Africa. Dr Jaimendra Singh Inkosi Albert Luthuli Central Hospital Durban, South Africa Pros and Cons of Stem Cell Sources and their availability in Africa Dr Jaimendra Singh Inkosi Albert Luthuli Central Hospital Durban, South Africa Introduction The ability to perform a haematopoietic stem

More information

Narrator: Transplants using stem cells from the blood, bone marrow or umbilical cord blood

Narrator: Transplants using stem cells from the blood, bone marrow or umbilical cord blood [Track 2: What Is a Transplant?] Narrator: Transplants using stem cells from the blood, bone marrow or umbilical cord blood can be an effective treatment for people with blood cancers such as leukemia,

More information

BioArchive Freezing Curve for Cord Blood

BioArchive Freezing Curve for Cord Blood BioArchive Freezing Curve for Cord Blood Determination of the Specific Freezing in the BioArchive System to Provide the Highest Viability of Cord Blood MNC as Measured by -C Assays Introduction Freezing

More information

CORD BLOOD BANKING IN BRAZIL HOSPITAL ISRAELITA ALBERT EINSTEIN S EXPERIENCE

CORD BLOOD BANKING IN BRAZIL HOSPITAL ISRAELITA ALBERT EINSTEIN S EXPERIENCE CORD BLOOD BANKING IN BRAZIL HOSPITAL ISRAELITA ALBERT EINSTEIN S EXPERIENCE JOSÉ MAURO KUTNER, MD MEDICAL DIRECTOR BLOOD BANK Brazil - South America BARCELONA * * SÃO PAULO Brazil - South America Brazil

More information

A Cure for Sickle Cell Anemia and Thalassemia

A Cure for Sickle Cell Anemia and Thalassemia IV Simpósio Internacional de Hemoglobinopatias A Cure for Sickle Cell Anemia and Thalassemia Bertram Lubin, MD and Mark Walters, MD 4 September 2007 Topics to be covered Cord blood: Importance and biology

More information

hematopoietic stem cells from cord blood: - costless but too expensive? Dr. Vincent Kindler Geneva Cord Blood Bank HUG vincent.kindler@hcuge.

hematopoietic stem cells from cord blood: - costless but too expensive? Dr. Vincent Kindler Geneva Cord Blood Bank HUG vincent.kindler@hcuge. hematopoietic stem cells from cord blood: - costless but too expensive? Dr. Vincent Kindler Geneva Cord Blood Bank HUG vincent.kindler@hcuge.ch Spring water: Manufactured by nature COSTLESS Cord Blood:

More information

The Process of Infusing Cryopreserved Stem Cells by Registered Nurses

The Process of Infusing Cryopreserved Stem Cells by Registered Nurses The Process of Infusing Cryopreserved Stem Cells by Registered Nurses Presenters: Kristen Brazel, RN BScN Kate Duke, RN BScN 5 West Haematology Unit, The Ottawa General Hospital, Ottawa, Ontario, Canada

More information

Dr Saeed Al Amoudi. Consultant Hematologist King s college hospital Director of Regional Lab Jeddah

Dr Saeed Al Amoudi. Consultant Hematologist King s college hospital Director of Regional Lab Jeddah Dr Saeed Al Amoudi Consultant Hematologist King s college hospital Director of Regional Lab Jeddah MAGIC BULLET Cord Blood Banking What is Cord Blood? Cord blood is the blood left in the placenta and umbilical

More information

Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport

Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport Cytotherapy (2006) Vol. 8, No. 2, 158/165 Fresh PBSC harvests, but not BM, show temperature-related loss of CD34 viability during storage and transport V Antonenas 1, F Garvin 1, M Webb 1, M Sartor 2,

More information

The Impact of Maternal, Neonatal and Collections factors on the TNC count of an Umbilical Cord Blood Donation

The Impact of Maternal, Neonatal and Collections factors on the TNC count of an Umbilical Cord Blood Donation The Impact of Maternal, Neonatal and Collections factors on the TNC count of an Umbilical Cord Blood Donation By: NHS Cord Blood Bank (Colindale Laboratory) Date: 26 th September 2014 NHS insert Cord your

More information

An Introduction to Canada s National Public Cord Blood Bank

An Introduction to Canada s National Public Cord Blood Bank An Introduction to Canada s National Public Cord Blood Bank Dr. Heidi Elmoazzen Director, National Public Cord Blood Bank Hematopoetic Stem Cells Hematopoetic stem cells are not yet mature (undifferentiated)

More information

Natasha Kekre, 1 Jennifer Philippe, 2 Ranjeeta Mallick, 3 Susan Smith, 2 and David Allan 1,2,4. 1. Introduction

Natasha Kekre, 1 Jennifer Philippe, 2 Ranjeeta Mallick, 3 Susan Smith, 2 and David Allan 1,2,4. 1. Introduction Hindawi Publishing Corporation Stem Cells International Volume 2013, Article ID 124834, 6 pages http://dx.doi.org/10.1155/2013/124834 Research Article Modelling Improvements in Cell Yield of Banked Umbilical

More information

Proliferation/Potency Assays: Is there more to CD34 and CFU?

Proliferation/Potency Assays: Is there more to CD34 and CFU? Proliferation/Potency Assays: Is there more to CD34 and CFU? CBMTG 2012 Laboratory Committee Meeting April 11, 2012 Toronto, ON, Canada Leah Marquez-Curtis CBS-Edmonton OUTLINE I. Rationale and Definitions

More information

Neutrophil Recovery: The First Step in Posttransplant Recovery

Neutrophil Recovery: The First Step in Posttransplant Recovery Neutrophil Recovery: The First Step in Posttransplant Recovery is Made in the Bone Stem Cell CFU- GEMM BFU-E Pre-B CFU-E White cells B Lymphocyte T Lymphocyte Neutrophils Red cells No conflicts of interest

More information

Bone Marrow, Peripheral Blood Stem Cells or Umbilical Cord Blood transplantation? Federica Giannotti, MD Eurocord-Hôpital Saint Louis, Paris

Bone Marrow, Peripheral Blood Stem Cells or Umbilical Cord Blood transplantation? Federica Giannotti, MD Eurocord-Hôpital Saint Louis, Paris Bone Marrow, Peripheral Blood Stem Cells or Umbilical Cord Blood transplantation? Federica Giannotti, MD Eurocord-Hôpital Saint Louis, Paris Background Hematopoietic stem cell transplantation (HSCT) is

More information

Umbilical Cord Blood Stem Cells Current Status & Future Potential

Umbilical Cord Blood Stem Cells Current Status & Future Potential Umbilical Cord Blood Stem Cells Current Status & Future Potential Natasha Ali Assistant Professor Haematology Department of Pathology & Laboratory Medicine/Oncology The Aga Khan University Email: natasha.ali@aku.edu

More information

Economics of Cord Blood Banking. Michael Boo NMDP Be The Match September 11, 2015

Economics of Cord Blood Banking. Michael Boo NMDP Be The Match September 11, 2015 Economics of Cord Blood Banking Michael Boo NMDP Be The Match September 11, 2015 Historical Use UCB Use Data 2 NMDP Historical Transplants by Product 7,000 6,500 6,000 5,500 5,000 4,500 4,000 3,500 3,000

More information

11/10/2011. History of CBUs. 10-CBA providing access to unlicensed cord blood units. The FDA s involvement. The FDA s involvement, cont.

11/10/2011. History of CBUs. 10-CBA providing access to unlicensed cord blood units. The FDA s involvement. The FDA s involvement, cont. History of CBUs First CBU transplant done in 1988 10-CBA providing access to unlicensed cord blood units Using the NMDP IND to access unlicensed cord blood units Amy Hays, Sr. Clinical Research Specialist,

More information

In contrast to the very high transplant-related

In contrast to the very high transplant-related Cord Blood: an Alternative Stem Cell Source or a New Standard? Juliet N. BARKER Memorial Sloan-Kettering Cancer Center, NY, ABD In contrast to the very high transplant-related mortality (TRM) associated

More information

Cord Blood Transplant. E. Gluckman Eurocord ESH-EBMT training course Vienna 2014

Cord Blood Transplant. E. Gluckman Eurocord ESH-EBMT training course Vienna 2014 Cord Blood Transplant E. Gluckman Eurocord ESH-EBMT training course Vienna 2014 Background Since 1988, umbilical cord blood (CB) has been successfully used to treat children and adults needing stem cell

More information

Transmedcon 2014. Ahmedabad, Gujarat November 14-16, 2014

Transmedcon 2014. Ahmedabad, Gujarat November 14-16, 2014 FACT Accreditation for Cord Blood Banks and Cellular Therapy Nadim Mahmud, MD, PhD Associate Professor of Medicine Division of Hematology/Oncology Director, Hospital Stem Cell Laboratory Blood & Marrow

More information

Blood-Forming Stem Cell Transplants

Blood-Forming Stem Cell Transplants Blood-Forming Stem Cell Transplants What are bone marrow and hematopoietic stem cells? Bone marrow is the soft, sponge-like material found inside bones. It contains immature cells known as hematopoietic

More information

Umbilical Cord Blood: An Alternative Allogeneic Stem Cell Source for Transplantation

Umbilical Cord Blood: An Alternative Allogeneic Stem Cell Source for Transplantation Umbilical Cord Blood: An Alternative Allogeneic Stem Cell Source for Transplantation Mary J. Laughlin, MD Associate Professor of Medicine and Pathology Dr. Donald and Ruth Weber Goodman Professor of Innovative

More information

A New Beginning: Stem Cell Transplant Challenges. Dr. Wendy Lau Director, Transfusion Medicine, The Hospital for Sick Children April 8, 2016

A New Beginning: Stem Cell Transplant Challenges. Dr. Wendy Lau Director, Transfusion Medicine, The Hospital for Sick Children April 8, 2016 A New Beginning: Stem Cell Transplant Challenges Dr. Wendy Lau Director, Transfusion Medicine, The Hospital for Sick Children April 8, 2016 Objectives 1. Outline cellular therapy products and transfusion

More information

Canadian Blood Services National Public Cord Blood Bank

Canadian Blood Services National Public Cord Blood Bank Canadian Blood Services National Public Cord Blood Bank Dr. Heidi Elmoazzen Director, National Public Cord Blood Bank Hematopoetic Stem Cells Hematopoetic stem cells are not yet mature (undifferentiated)

More information

Bone Marrow Transplantation and Peripheral Blood Stem Cell Transplantation: Questions and Answers. Key Points

Bone Marrow Transplantation and Peripheral Blood Stem Cell Transplantation: Questions and Answers. Key Points CANCER FACTS N a t i o n a l C a n c e r I n s t i t u t e N a t i o n a l I n s t i t u t e s o f H e a l t h D e p a r t m e n t o f H e a l t h a n d H u m a n S e r v i c e s Bone Marrow Transplantation

More information

BioPreservation Today

BioPreservation Today BioPreservation Today Volume 1 - Issue 4 FALL 2009 published by BioLife Solutions, Inc. FEATURE ARTICLE CASE STUDY: MODIFIED CRYOPRESERVATION OF UMBILICAL CORD BLOOD STEM CELLS AT CRYOBANKS INTERNATIONAL

More information

Review. Cord blood as an alternative source of haemopoietic stem cells. J. Hows, A. Nicol, P. Denning-Kendall, C. Donaldson, M. Nieda & B.

Review. Cord blood as an alternative source of haemopoietic stem cells. J. Hows, A. Nicol, P. Denning-Kendall, C. Donaldson, M. Nieda & B. Annals of Oncology 7 (Suppi 2): 47-51, 1996. O 1996 Kluwer Academic Publishers. Printed in the Netherlands. Review Cord blood as an alternative source of haemopoietic stem cells J. Hows, A. Nicol, P. Denning-Kendall,

More information

OneMatch National. Sue Smith, Executive Director, Stem Cells 2011-11- 04

OneMatch National. Sue Smith, Executive Director, Stem Cells 2011-11- 04 OneMatch National Public Cord Blood Bank Blood Matters 2011 Sue Smith, Executive Director, Stem Cells 2011-11- 04 No Disclosures To Declare The presenter does not have any involvement with the industry

More information

Stem Cell Background Paper

Stem Cell Background Paper Stem Cell Background Paper Introduction...2 Stem Cell Basics...3 Stem Cell Process Flow...9 Comparison of Blood, Stem Cells, Tissues and Organs Processes...10 Responsibilities for the Blood, Stem Cells,

More information

Overview of OneMatch, including the OneMatch Public Cord Blood Bank

Overview of OneMatch, including the OneMatch Public Cord Blood Bank Overview of OneMatch, including the OneMatch Public Cord Blood Bank Dr. Heidi Elmoazzen, Director OneMatch Public Cord Bank 2012-09-27 Outline Overview and Introduction Overview of the need for stem cells

More information

Graft Failure After HSCT

Graft Failure After HSCT Graft Failure After HSCT Vanderson Rocha, MD, PhD Professor of Haematology- Oxford University Bone Marrow Transplant Unit- Sirio Libanes Hospital- Sao Paulo Scientific Director of Eurocord-Paris Clinical

More information

Cord Blood Transplant Past and Future. E. Gluckman Eurocord ISCT Paris 24/04/2014

Cord Blood Transplant Past and Future. E. Gluckman Eurocord ISCT Paris 24/04/2014 Cord Blood Transplant Past and Future E. Gluckman Eurocord ISCT Paris 24/04/2014 Background Since 1988, umbilical cord blood (CB) has been successfully used to treat children and adults needing stem cell

More information

HPC, Cord Blood Injectable Suspension for Intravenous Use Initial U.S. Approval: 2012

HPC, Cord Blood Injectable Suspension for Intravenous Use Initial U.S. Approval: 2012 HIGHLIGHTS OF PRESCRIBING INFORMATION These highlights do not include all the information needed to use HPC, Cord Blood safely and effectively. See full prescribing information for HPC, Cord Blood. HPC,

More information

STEM CELL TRANSPLANTS

STEM CELL TRANSPLANTS UAMS Information on STEM CELL TRANSPLANTS What is a Stem Cell Transplant? A stem cell transplant is an infusion of stem cells following high-dose chemotherapy. The infused cells effectively rescue the

More information

Product Complaint Form Instructions

Product Complaint Form Instructions Product Complaint Form Instructions Note: Product Complaint Definition: Failure/possible failure of a drug (includes biological products) to meet any of its specifications. This includes complaints that

More information

Corporate Medical Policy Cord Blood as a Source of Stem Cells

Corporate Medical Policy Cord Blood as a Source of Stem Cells Corporate Medical Policy Cord Blood as a Source of Stem Cells File Name: Origination: Last CAP Review: Next CAP Review: Last Review cord_blood_as_a_source_of_stem_cells 2/2001 3/2015 3/2016 3/2015 Description

More information

CBS National Public Cord Blood Bank. Did you donate your cord blood?

CBS National Public Cord Blood Bank. Did you donate your cord blood? CBS National Public Cord Blood Bank Did you donate your cord blood? Tanya Petraszko MD FRCPC October 2014 Overview What is a stem cell? The importance of HLA Need for cord blood stem cells For all Canadians

More information

Umbilical Cord Blood Banks and Banking

Umbilical Cord Blood Banks and Banking 4 Umbilical Cord Blood Banks and Banking The centers that collect, process, and store umbilical cord blood differ in their organization and governance as well as in the cord blood processing methods that

More information

Review. Plasma-Depleted Versus Red Cell-Reduced Umbilical Cord Blood

Review. Plasma-Depleted Versus Red Cell-Reduced Umbilical Cord Blood Cell Transplantation, Vol. 23, pp. 407 415, 2014 0963-6897/14 $90.00 +.00 Printed in the USA. All rights reserved. DOI: http://dx.doi.org/10.3727/096368914x678481 Copyright 2014 Cognizant Comm. Corp. E-ISSN

More information

Summary Basis for Regulatory Action

Summary Basis for Regulatory Action Date: June 13, 2013 Summary Basis for Regulatory Action From: Mohammad Heidaran, Ph.D., Chair of the Review Committee BLA/ STN#: 125432 Applicant Name: LifeSouth Community Blood Centers, Inc. Date of Submission:

More information

Pl I enary Tr T anslational Medicine April 30, 2015

Pl I enary Tr T anslational Medicine April 30, 2015 Plenary I Translational Medicine April 30, 2015 HBO in Hematopoietic Stem Cell Transplantation: Lessons Learned Omar Aljitawi, MD Hematology/Oncology Hematology and Transplantation (HAT) Laboratory Pathology

More information

The Value of Cord Blood Stem Cells. Mona Shafey, MD, FRCPC Medical Grand Rounds October 25 th, 2011

The Value of Cord Blood Stem Cells. Mona Shafey, MD, FRCPC Medical Grand Rounds October 25 th, 2011 The Value of Cord Blood Stem Cells Mona Shafey, MD, FRCPC Medical Grand Rounds October 25 th, 2011 Objectives To discuss umbilical cord blood as a stem cell source and the role of umbilical cord blood

More information

CHAPTER 1 BACKGROUND AND CORD BLOOD BANK (CBB) ORGANIZATION

CHAPTER 1 BACKGROUND AND CORD BLOOD BANK (CBB) ORGANIZATION CHAPTER 1 BACKGROUND AND CORD BLOOD BANK (CBB) ORGANIZATION Chapter 1 BACKGROUND AND CORD BLOOD BANK (CBB) ORGANIZATION 1.1 OVERVIEW OF THE CORD BLOOD TRANSPLANTATION STUDY Bone marrow transplantation

More information

Review Article A Review of Factors Influencing the Banking of Collected Umbilical Cord Blood Units

Review Article A Review of Factors Influencing the Banking of Collected Umbilical Cord Blood Units Stem Cells International Volume 2013, Article ID 463031, 7 pages http://dx.doi.org/10.1155/2013/463031 Review Article A Review of Factors Influencing the Banking of Collected Umbilical Cord Blood Units

More information

Public Umbilical Cord Blood Banking at Canadian Blood Services. David Allan, MD Medical Director, National Public Cord Blood Bank 6 May 2015

Public Umbilical Cord Blood Banking at Canadian Blood Services. David Allan, MD Medical Director, National Public Cord Blood Bank 6 May 2015 Public Umbilical Cord Blood Banking at Canadian Blood Services David Allan, MD Medical Director, National Public Cord Blood Bank 6 May 2015 Overview Quick review of stem cell transplant activity Cord blood

More information

cord blood saves lives...

cord blood saves lives... cord blood saves lives... ...by providing a rich source of blood stem cells that can be used to treat over 80 different diseases including leukemia, lymphoma, thalassemia, Tay-Sachs and sickle cell anemia.

More information

Stem Cell Transplantation In Patients with Fanconi Anemia

Stem Cell Transplantation In Patients with Fanconi Anemia Stem Cell Transplantation In Patients with Fanconi Anemia FARF Annual Family Meeting 6/28/15 Casco, ME Parinda A. Mehta, M.D. Cincinnati Children s Hospital Medical Center Improvements in Unrelated Donor

More information

Kathie Viers-City of Hope Eric Presson- Baylor Health Care Systems Jennifer Christian-Markay Cancer Center

Kathie Viers-City of Hope Eric Presson- Baylor Health Care Systems Jennifer Christian-Markay Cancer Center Kathie Viers-City of Hope Eric Presson- Baylor Health Care Systems Jennifer Christian-Markay Cancer Center Discuss Quality dashboards as auditing and Reporting Methods for Quality Management Provide examples

More information

SAVE A LIFE... BY GIVING LIFE!

SAVE A LIFE... BY GIVING LIFE! SAVE A LIFE... BY GIVING LIFE! FOLLOW US ON: HÉMA-QUÉBEC PUBLIC CORD BLOOD BANK www.hema-quebec.qc.ca Scan this code with your smart phone to access the page Register to the Public Cord Blood Bank on the

More information

Disclosures. I have no disclosures.

Disclosures. I have no disclosures. Not Your Own Marrow Jenni Krajewski, MD Clinical Assistant Professor, Rutgers New Jersey Medical School Attending Physician, Pediatric Blood and Marrow Transplantation The Institute for Pediatric Cancer

More information

Hematopoietic Stem Cell Transplantation. Imad A. Tabbara, M.D. Professor of Medicine

Hematopoietic Stem Cell Transplantation. Imad A. Tabbara, M.D. Professor of Medicine Hematopoietic Stem Cell Transplantation Imad A. Tabbara, M.D. Professor of Medicine Hematopoietic Stem Cells Harvested from blood, bone marrow, umbilical cord blood Positive selection of CD34 (+) cells

More information

Determinants of Engraftment after Double-Unit Cord Blood Transplantation

Determinants of Engraftment after Double-Unit Cord Blood Transplantation In: Broxmeyer HE, ed. Cord Blood: Biology, Transplantation, Banking, and Regulation Bethesda, MD: AABB Press, 2011 26 Determinants of Engraftment after Double-Unit Cord Blood Transplantation Doris M. Ponce,

More information

HEMACORD (HPC, Cord Blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: 2011

HEMACORD (HPC, Cord Blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: 2011 HIGHLIGHTS OF PRESCRIBING INFORMATION These highlights do not include all the information needed to use HEMACORD safely and effectively. See full prescribing information for HEMACORD. HEMACORD (HPC, Cord

More information

4. All cord blood banks should be subject to the same standards, regulations and accreditation requirements.

4. All cord blood banks should be subject to the same standards, regulations and accreditation requirements. WMDA Policy Statement on the Utility of Autologous or Family Cord Blood Unit Storage The WMDA Board adopted this policy on 25 th of May 2006. Policy updated _April 2011 The Cord Blood Working Group and

More information

Stephen J. Szilvassy, PhD Associate Director, Hematopoietic Products R&D STEMCELL Technologies Inc., Vancouver, Canada WWW.STEMCELL.

Stephen J. Szilvassy, PhD Associate Director, Hematopoietic Products R&D STEMCELL Technologies Inc., Vancouver, Canada WWW.STEMCELL. WHITE PAPER Potency Assays for Measuring the Engraftment Potential of Hematopoietic Stem and Progenitor Cells Stephen J. Szilvassy, PhD Associate Director, Hematopoietic Products R&D STEMCELL Technologies

More information

Selecting an appropriately matched donor for hematopoietic

Selecting an appropriately matched donor for hematopoietic Transplant Outcomes in Acute Leukemia (I) Mary Eapen a and John E. Wagner b Umbilical cord blood (UCB) has gradually emerged over the last decade as an alternative source of hematopoietic cells for transplantation

More information

HEMACORD (HPC, Cord Blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: 2011

HEMACORD (HPC, Cord Blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: 2011 HIGHLIGHTS OF PRESCRIBING INFORMATION These highlights do not include all the information needed to use HEMACORD safely and effectively. See full prescribing information for HEMACORD. HEMACORD (HPC, Cord

More information

ALBERTA BLOOD AND MARROW TRANSPLANT PROGRAM NEW GUIDELINES FOR THE IMMUNIZATION OF HEMATOPOIETIC STEM CELL TRANSPLANT(HSCT) RECIPIENTS JULY 11, 2013

ALBERTA BLOOD AND MARROW TRANSPLANT PROGRAM NEW GUIDELINES FOR THE IMMUNIZATION OF HEMATOPOIETIC STEM CELL TRANSPLANT(HSCT) RECIPIENTS JULY 11, 2013 ALBERTA BLOOD AND MARROW TRANSPLANT PROGRAM NEW GUIDELINES FOR THE IMMUNIZATION OF HEMATOPOIETIC STEM CELL TRANSPLANT(HSCT) RECIPIENTS JULY 11, 2013 RE-IMMUNIZATION IS IMPORTANT! There should be no assumptions

More information

INFORMATION ON STEM CELLS/BONE MARROW AND REINFUSION/TRANSPLANTATION SUR703.002

INFORMATION ON STEM CELLS/BONE MARROW AND REINFUSION/TRANSPLANTATION SUR703.002 INFORMATION ON STEM CELLS/BONE MARROW AND REINFUSION/TRANSPLANTATION SUR703.002 COVERAGE: SPECIAL COMMENT ON POLICY REVIEW: Due to the complexity of the Peripheral and Bone Marrow Stem Cell Transplantation

More information

5. All cord blood banks should be subject to the same standards, regulations and accreditation requirements.

5. All cord blood banks should be subject to the same standards, regulations and accreditation requirements. WMDA Policy Statement for the Utility of Autologous or Family Cord Blood Unit Storage (This policy statement has been approved and adopted by the WMDA board on the 25 th of May 2006) The Cord Blood Registries

More information

Equine Stem Cells. Sources, Processing, Expansion, Storage and Shipping

Equine Stem Cells. Sources, Processing, Expansion, Storage and Shipping Equine Stem Cells Sources, Processing, Expansion, Storage and Shipping Sean Owens, DVM, Diplomate ACVP Medical Director, UC Davis Veterinary Regenerative Medicine Laboratory What is a Stem Cell and What

More information

Canadian Blood Services National Public Cord Blood Bank Give Life Twice Transfusion Medicine Residents

Canadian Blood Services National Public Cord Blood Bank Give Life Twice Transfusion Medicine Residents Canadian Blood Services National Public Cord Blood Bank Give Life Twice Transfusion Medicine Residents Eileen Quinlan Collection Supervisor, Brampton (GTA) 2015-11-10 History One Match Stem Cell and Marrow

More information

Enumeration and Viability of Nucleated Cells from Bone Marrow, Cord Blood, and Mobilized Peripheral Blood

Enumeration and Viability of Nucleated Cells from Bone Marrow, Cord Blood, and Mobilized Peripheral Blood Application Note Primary Cell Analysis Enumeration and Viability of Nucleated Cells from Bone Marrow, Cord Blood, and Mobilized Peripheral Blood Cell Viability Introduction: Bone Marrow, Cord Blood, Stem

More information

Automated and Standardized Counting of CFU Assays for Cord Blood Banks

Automated and Standardized Counting of CFU Assays for Cord Blood Banks Automated and Standardized Counting of CFU Assays for Cord Blood Banks 2 Automated and Standardized Colony Counting Table of Contents 4 The Colony-Forming Unit (CFU) Assay for Cord Blood Banks 5 The CFU

More information

Milano Cord Blood Bank 18 year report on cord blood banking and transplant

Milano Cord Blood Bank 18 year report on cord blood banking and transplant Milano Cord Blood Bank 18 year report on cord blood banking and transplant Paolo Rebulla, MD Milano Cord Blood Bank Center of Transfusion Medicine, Cellular Therapy and Cryobiology Department of Regenerative

More information

10 th Annual International Cord Blood Transplantation Symposium June 7-9, 2012 Preliminary Scientific Program. Thursday, June 7

10 th Annual International Cord Blood Transplantation Symposium June 7-9, 2012 Preliminary Scientific Program. Thursday, June 7 10 th Annual International Cord Blood Transplantation Symposium June 7-9, 2012 Preliminary Scientific Program Thursday, June 7 7:00 8:00 AM Breakfast in Exhibit Hall Session IA 8:00 10:00 AM Advances in

More information

Fetal Maternal Immunity and Antileukemia Activity in Cord Blood Transplant. Recipients

Fetal Maternal Immunity and Antileukemia Activity in Cord Blood Transplant. Recipients Fetal Maternal Immunity and Antileukemia Activity in Cord Blood Transplant Recipients Filippo Milano, 1 J. Lee Nelson, 1, 2 Colleen Delaney 1,3 1 Clinical Research Division, Fred Hutchinson Cancer Research

More information

HEMACORD (hematopoietic progenitor cells, cord blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: XXXX

HEMACORD (hematopoietic progenitor cells, cord blood) Injectable Suspension for Intravenous Use Initial U.S. Approval: XXXX HIGHLIGHTS OF PRESCRIBING INFORMATION These highlights do not include all the information needed to use HEMACORD safely and effectively. See full prescribing information for HEMACORD. HEMACORD (hematopoietic

More information

Cord Blood: Research Progress and Future Promise

Cord Blood: Research Progress and Future Promise Cord Blood: Research Progress and Future Promise By Al Staropoli, AABB Contributing Writer Sue Fister found out she had leukemia when she was 50 years old. Doctors suggested a bone marrow transplant as

More information

Improved Ex Vivo Expansion of Functional CD34 + Cells Using Stemline II Hematopoietic Stem Cell Expansion Medium

Improved Ex Vivo Expansion of Functional CD34 + Cells Using Stemline II Hematopoietic Stem Cell Expansion Medium Improved Ex Vivo Expansion of Functional CD34 + Cells Using Stemline II Hematopoietic Stem Cell Expansion Medium Daniel W. Allison, 1 Stacy L. Leugers, 1 Barry J. Pronold, 2 Gary Van Zant, 2 Jenny A. Harrington,

More information

Jennifer G. Collins, RN Children s Hospital of Chicago

Jennifer G. Collins, RN Children s Hospital of Chicago Jennifer G. Collins, RN Children s Hospital of Chicago Review the importance of consultation with the stem cell transplant team Review criteria to begin harvest Review ways to modify a harvest procedure

More information