Solvents and Formulations for Liquid Chromatography

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1 Solvents and Formulations for Liquid Chromatography Because You need Quality! Solvents and Formulations for Liquid Chromatography LEENDERWEG 78, 5555 CE VALKENSWAARD, THE NETHERLANDS Tel: , Fax: Website:

2 HPLC Grade LC/MS Grade ULC/MS Grade

3 For over three decades, Bio-Lab Ltd, an ISO 9001 and ISO company, has been Thanks to constant effort in R&D, we were able to put on the market over the years exceptionally high quality products resulting HPLC Grade In high performance liquid chromatography the producing high purity solvents, reagents in guaranteed and often unique specifications. intrinsic quality of the solvents is of the greatest and formulations which are distributed It also has been our task to adapt the significance. We have been optimizing our through its subsidiary, Biosolve in Benelux, specifications of selected solvents and HPLC grades to match many LC applications, France and Germany. formulations to the customer's particular use. enabling you to determine which is the best Quality assurance means the world to us. We can offer you a full range of solvents, grade for your specific need. Our QA policy is expressed from the initial reagents and formulations that are considered Gradient grade HPLC solvents are specified acceptance of the selected raw material the best available products on the LC and with the gradient elution tests for the highest through the in process control to the LC/MS market. peak and drift at critical wavelengths in order professionally analyzed finished products. to demonstrate the peak-free baseline. This All steps of production are perfectly enables you to make a better evaluation of documented and traceable, and final minor impurities assuring optimum sensitivity. distillations are performed in all-glass equipment ensuring high quality chemicals Biosolve HPLC solvents are lot-to-lot analyzed with lot-to-lot reproducibility. Certificates to meet the high quality specifications for HPLC of analysis and compliance (COA and COC) are available on request, even for long past delivered products. Continuous training of our personnel, and upgrading manufacturing and laboratory operative procedures will continue to lead us towards both our goals: highest quality and environmental Thanks to constant effort in R&D, we were able to put on the market over the years exceptionally high quality products resulting in guaranteed and often unique specifications. separations. We control the low levels of peak impurities, residue, water, acidity and alkalinity, the high chemical purity and the UV transmittance, all of which are critical parameters for a successful HPLC application. All our HPLC solvents are filtered through 0.2 µm membranes, insuring particle-free mobile phases, and are bottled under inert gas We control the low levels of peak impurities, residue, water, acidity and alkalinity, the high chemical purity and the UV transmittance, all of which are critical parameters for a successful HPLC application. care. for improved shelf life. HPLC Grade

4 HPLC Solvents, Reagents and Formulations HPLC Solvents, Reagents and Formulations UV Transmit-tance Acidity (as AcOH or HCl) UV Transmit-tance Acidity (as AcOH or HCl)

5 HPLC Solvents, Reagents and Formulations HPLC Solvents, Reagents and Formulations UV Transmit- -tance Acidity (as AcOH or HCl) UV Transmit- -tance Acidity (as AcOH or HCl)

6 LC/MS Grade Mass spectrometers work by ionizing To avoid such misleading phenomena, it is LC/MS Solvents & formulations / Purity H. Peak at UV Transmittance Ionic Metals max. ppm molecules, and then sorting and identifying the important to use solvents containing minimum ions according to their mass-to-charge (m/z) concentrations of such metal ions. Moreover, ratios. Common atmospheric pressure impurities arising from the solvents may mask ionization techniques are: low levels of target ion molecules, which will Electrospray ionization (ESI) result in faulty interpretation of the Atmospheric pressure chemical ionization chromatograms. (APCI) Biosolve LC/MS solvents are manufactured Atmospheric pressure photoionization (APPI) specifically to meet the needs of modern These instruments have two key components: LC/MS instruments, checked and guaranteed the ion source, which generates the ions, and to contain low levels of contaminants and ionic the mass analyzer, which sorts the ions. Much background. Specific UV tests and gradient of the advancement in LC/MS over the last elution control are also guaranteed to ensure decade has been in the development of ion reproducibility. sources and techniques that ionize the analyte All LC/MS solvents are filtered through 0.2 molecules and separate the resulting ions from µm, insuring particle-free mobile phases and the mobile phase. are bottled under inert gas for improved shelf When ionization occurs in the presence of life. Alkali metal, some components are susceptible to form adducts or clusters that may mask the true molecular MH+ ions. Biosolve LC/MS solvents are manufactured specifically to meet the needs of modern LC/MS instruments, checked and guaranteed to contain low levels of contaminants and ionic background. Specific UV tests and gradient elution control are also guaranteed to ensure reproducibility. LC/MS Grade

7 ULC/MS Grade ULC/MS Solvents Recent improvements on the High and For routine work, we also offer ready-made Ultra PLC instruments, coupled with sensitive ULC/MS formulations, such as: Trifluoracetic MS, PDA, ELSD, CAD, etc. detectors, have acid, Formic acid, Acetic acid and Ammonium led to special high-performing systems. Ultra acetate in Water, Acetonitrile and Methanol low detection limits and valid analysis of mixtures. These additives are used to improve molecular structures of proteins, peptides, chromatographic peak shape and to optimize oligonucleotides and other chemicals brought ionization in the MS interface. these new techniques a growing popularity, Ultra pure salts and acids are also available especially in the pharmaceutical and for mobile phase preparation. We are ready biotechnology industry. to offer our help in tailor-made formulations to Some minor impurities arising from the solvents conform to your specific needs. Fluores- UV Transmit- Ionic Metals cence Drift at H. Peak at tance max. ppb nm/max. ppb may interfere with low levels of sample impurities, or give false peaks, which will result Biosolve ULC/MS solvents and formulations in inadequate interpretation of the are micro filtered at 0.1 µm, and have a very chromatograms. low residue on evaporation, offering the best Biosolve Ultra Liquid Chromatography / Mass protection for your columns and detectors. All Spectrometry solvents (ULC/MS) for high ULC/MS reagents are packed under inert gas resolution and sensitivity were developed in for improved shelf life. close cooperation with advanced instrument companies to meet these new requirements. Biosolve ULC/MS solvents are checked and guaranteed to meet the demand for: High chemical purity, high UV transmission, lowest peak impurities and drift in the gradient elution tests to ensure reproducibility, low fluorescence impurities and low level of ionic background of less than 100 ppb of Alkali metal. ULC/MS Formulations High chemical purity, high UV transmission, lowest peak impurities and drift in the gradient elution tests to ensure reproducibility. Low fluorescence impurities and low level of ionic background of less than 100 ppb of Alkali metal. ULC/MS Grade Purity Fluores- Water UV Transmit- Ionic Metals cence Drift at H. Peak at (KF) tance max. ppb nm/max. ppb

8 ULC/MS Acids & Salts Fluorescence nm/max. ppb Drift at Water H. Peak at (KF) HPLC Gradient elution 3D chromatogram from 210nm to 400nm. Acetonitrile ULC/MS batch # and Water ULC/MS batch # System parameters: Column RP-18 spherical 5µm, 254x4.6mm, PDA 2998 detector. Gradient elution test parameters: Loading of 100% ACN for 5 min. Back program to 5% ACN in 2 min. Column equilibrium for 3 min. Gradient elution from 5% to 100% ACN in 20 min. Hold at 100% ACN for 10 min UV Transmittance Ionic Metals max. ppm Water ULC/MS Biosolve early recognized the importance of Methanol) or inherent to the analyzed sample. Water for sophisticated LC and LC-MS Over the years, the Water quality at Biosolve applications. has been constantly upgraded and at present, Minor impurities present in the water often our Water ULC/MS is produced by not less "charge" the LC columns at the early stage of than 11 monitored purification steps, including the gradient chromatographic run. short UV treatment and final filtration through Such impurities may then be released as single 0.1 µm membranes. or multiple peaks with rising gradient conditions. Our bottles are selected and treated to The operator may then consider the presence minimize ion release from the internal glass of such peak(s) as impurity(ies) that are present surface. The filling is performed under aseptic in the mobile phase co-solvent (e.g. Acetonitrile, conditions. PDA max plot gradient chromatogram from 210nm to 400nm. Acetonitrile ULC/MS batch # and Water ULC/MS batch # *For complete information on product specifications and standard packaging, please refer to our internet website at: