Table of contents. I. Description...2. Principle...2. Content...3. Storage...4. V. Features...4. Precautions for Use...4. Protocol...

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Table of contents. I. Description...2. Principle...2. Content...3. Storage...4. V. Features...4. Precautions for Use...4. Protocol..."

Transcription

1 Table of contents I. Description...2 II. III. IV. Principle...2 Content...3 Storage...4 V. Features...4 VI. VII. Precautions for Use...4 Protocol...4 A) Protocol using Smart Cycler II System...4 B) Protocol using ABI PRISM 7000/7700/7900 HT & 7300/7500/7500 Fast Real-Time PCR System...7 C) Protocol using LightCycler...9 D) Protocol using Mx3000P VIII. Application Example IX. Related products

2 I. Description Premix Ex Taq (Perfect Real Time) is a 2 convenient premix reagent, specially designed for real time PCR by using TaqMan * 1 probe. This product combines the high performance of TaKaRa Ex Taq HS, which is an enzyme for hot start PCR utilizing Taq antibody, with a newly developed buffer which provides superior specificity, increased amplification efficiency and high aptitude for high-speed real time PCR. Accordingly, a successful real time PCR is promised with high sensitivity, wide dynamic range and accurate quantification. II. Principle Applicable real time PCR instruments; ABI PRISM 7000, 7700, 7900HT, *2 Applied Biosystems 7300, 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System (Life Technologies) LightCycler (Roche Diagnostics) *3 Smart Cycler System/Smart Cycler II System (Cepheid) *4 Mx3000P (Stratagene) Thermal Cysler Dice Real Time System II (TaKaRa Bio) *5 * 1 : TaqMan is a trademark of Roche Molecular Systems Inc. * 2 : ABI PRISM is a registered trademark of Applera Corporation. * 3 : LightCycler is a registered trademark of Roche Diegnostics. * 4 : Smart Cycler is a trademark of Cepheid. * 5 : This instrument is not available in the U.S. and Europe. This product employs TaKaRa Ex Taq HS for PCR reaction. PCR products are detected with TaqMan probe in real time monitoring. 1) PCR PCR (Polymerase Chain Reaction) is a simple and powerful method to amplify only a target DNA of a tiny amount by cycling three incubation steps at different temperatures: Double-stranded target DNA is heat denatured (denaturation step), the two primers complementary to the target segment are annealed at low temperature (annealing step), and the annealed primers are then extended at an intermediate temperature (extension step) with a DNA polymerase.as the target copy number doubles upon each cycle, PCR can thereby amplify DNA fragments up to fold in a short period. As this product utilizes an enzyme for Hot Start PCR, TaKaRa Ex Taq HS, non-specific amplification due to mispriming prior to cycling or due to primer dimmer can be minimized. Accordingly the highly specific and sensitive detection is achieved. 2) Fluorescence detection : TaqMan Probe method : The TaqMan method is based on a combination of TaqMan Technology and a real time PCR instrument. Oligonucleotide modified with fluorescence substance (e.g. FAM) at 5' -end and with quencher (e.g. TAMRA) at 3' -end is added in a reaction system. Under the annealing condition, TaqMan probe specifically hybridizes to template DNA, but the fluorescence is suppressed by quencher. In extension step, the 5' 3' exonuclease activity of Taq DNA polymerase degrades the TaqMan probe hybridized to a template. Accordingly, TaqMan probe is released from the suppression with a quencher, resulting in emission of fluorescence. By measuring the fluorescence intensity the amount of amplified product can be monitored. 2

3 1) Heat denaturation Primer Fluorescence Substance F Q Probe Quencher 2) Primer annealing/probe hybridization Polymerase F Hybridizes Q 3) Extension F Q F Q III. Content (250 units) Premix Ex Taq (Perfect Real Time) (2 conc.) *1 ROX Reference Dye (50 conc.) *2 ROX Reference Dye II (50 conc.) *2 1.0 ml 5 tubes 200 μl 200 μl * 1 : contains TaKaRa Ex Taq HS, dntp Mixture,and Mg 2+. * 2 : ROX Reference Dye/Dye II is used for normalization of intensity by background subtraction. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR System, the use of ROX Reference Dye (50 ) is recommended. For Applied Biosystems 7500 Real-Time PCR System, 7500 Fast Real-Time PCR System, and Stratagene Mx3000P, the use of ROX Reference Dye II is recommended. It is not required for use with Smart Cycler, LightCycler or Thermal Cycler Dice Real Time System II. Reagents and Instruments Required but Not Supplied in this product 1. Thermal Cycler for real time PCR (authorized instruments) 2. Reaction tube or plate for real time PCR 3. PCR primers 4. TaqMan probe for detection (licensed probes) 5. dh2o 6. Micropippets and Micropippet tips (autoclaved prior to use) 3

4 IV. Storage Stable at 4 for 6 months. Careful attention should be made not to cause contamination. * This product is shipped at -20. After delivered, store at 4. Make the concentration uniform by gently inverting a tube before use. * This product can be stored at -20 for long term storage. Once thawed, store at 4 and use up within 6 months. V. Features 1) Quick & accurate detection and quantification of target gene through real time PCR. 2) Easy-to-use 2 premix reagent designed for reducing pipetting steps. 3) High amplification efficiency and highly sensitive detection : This product utilizes an enzyme for hot start, TaKaRa Ex Taq HS. As this enzymebuffer system is optimized for real time PCR, this product offers highly efficient amplification and high sensitive detection. VI. Precautions for Use Please ensure to read through the following precautions prior to starting the protocol. 1) Prior to use, make the concentration uniform by gently inverting a tube before use. 2) A precipiate may form in this product during storage at -20. This precipitate can be completely dissolved by briefly warming the tube with your hands or placing the tube at room temperature, followed by inversion of the tube several times to mix until dissolved. USE THE REAGENT ONLY AFTER COMPLETELY REDISSOLVING THE PRECIPITATE to ensure a uniform concentration of components.do not vortex. Even in the absence of a precipitate, gentle mixing of the product (avoiding air bubbles) is recommended to provide a uniform concentration of components prior to use. 3) TaqMan probe for detection should be prepared separately. It is not supplied in this product. 4) For preparing and dispensing the reagents, a new disposable tip should be used to minimize contamination among samples. VII. Protocol A) Protocol using Smart Cycler II System 1. Prepare the following PCR reaction mixture. <per reaction> [ Reagents ] [ Volume ] [ Final concentration ] Premix Ex Taq (2 ) 12.5 μl 1 PCR Forward Primer (10 μm) 0.5 μl 0.2 μm *1 PCR Reverse Primer (10 μm) 0.5 μl 0.2 μm *1 TaqMan probe 1 μl *2 template 2 μl *3 dh2o 8.5 μl total 25 μl 4

5 * 1 : The final concentration of primers can be 0.2 μm in most reactions. When it does not work, determine the optimalconcentrations within the range μm. * 2 : Probe concentration differs depending on kind of real time PCR instrument, and kind of fluorescence labeling material. The adding amount should be determined by referring to the operation manual of instrument and a product insert supplied with probe. Generally when performing detection with Smart Cycler System, probe concentration should be determined to have the final concentration within the range μm. * 3 : Final template concentration varies depending on the copy number of target present in the template solution. Optimal amount should be determined by preparing the dilution series. DNA template should be used in less than 100 ng. When the RT reactant (cdna) is used as a template, it should be added in less 10% volume of PCR reaction mixture. 2. Start the reaction. Gently centrifuge the reaction tubes using the centrifuge exclusive use for Smart Cycler. Load the reaction tubes on Smart Cycler II System and start the reaction. Shuttle PCR standard protocol (below) is recommended. Try this protocol firstly, and optimize the reaction condition if needed. Shuttle PCR Standard Protocol Stage 1 : Initial Denaturation Stage 2 : PCR reaction Note : This product combines the high performance of TaKaRa Ex Taq HS, which is an enzyme for hot start PCR utilizing Taq antibody. Initial denaturation step prior to PCR should be at 95 for 30 sec. No need to heat at 95 for (5 - ) 15 min. as the initial denaturation, required for chemically modified Taq polymerase. If excessive heat treatment is provided, the enzyme activity descreases and the amplification efficiency and the accuracy in quantification can also be affected. 5

6 Initial Denaturation Step Temperature Time Detection Remark Initial Denaturation sec. OFF It is recommended to perform initial denaturation at 95 for 30 seconds, which is enough even when the template is genomic DNA or circular plasmid. Denaturation condition that exceeds 2 minutes unstabilizes the reaction thus not recommended. Shuttle PCR Step Temperature Time Detection Remark Denaturation sec. OFF Annealing/ Extension sec. ON General amplified size for real time PCR is less than 300 bp, so denaturation at 95 for 3-5 seconds would be sufficient. Please try at 60 for 20 seconds at first. The temperature should be optimized within the range of if optimization is required. When reaction does not proceed efficiently, extend the time. Cycle number ; cycles 3. After the reaction is completed, perform analysis. After the reaction is completed, verify the amplification curve. Establish the standard curve when quantification is done. * Refer to the operation manual of Smart Cycler for the analysis method with Smart Cycler. 6

7 B) Protocol using ABI PRISM 7000/7700/7900HT or Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System 1. Prepare PCR reaction (below). < per reaction > [ Reagents ] [ Volume ] [ Volume ] [ Final conc. ] Premix Ex Taq (2 ) 10 μl 25 μl 1 PCR Forward Primer (10 μm) 0.4 μl 1 μl 0.2 μm * 1 PCR Reverse Primer (10 μm) 0.4 μl 1 μl 0.2 μm * 1 TaqMan probe 0.8 μl 2 μl * 2 ROX Reference Dye or Dye II (50 ) * μl 1 μl 1 template 2 μl 4 μl * 4 dh2o 6 μl 16 μl total 20 μl * 5 50 μl * 5 * 1 : The final concentration of primers can be 0.2 μm in most reactions. When it does not work, determine the optimal concentrations within the range μm. * 2 : Probe concentration differs depending on kind of real time PCR instrument, and kind of fluorescence labeling material. The adding amount should be determined by referring to the operation manual of instrument and a product insert supplied with probe. * 3 : The ROX Reference Dye/Dye II is supplied for performing normalization of fluorescent signal intensities among wells when used with real time PCR instruments that have option. For ABI PRISM 7000/7700/7900HT and Applied Biosystems 7300 Real-Time PCR Syatem, the use of ROX Reference Dye (50 ) is recommended. For Applied Biosystems 7500 Real-Time PCR System and 7500 Fast Real- Time PCR System, the use of ROX Reference Dye II is recommended. * 4 : Final template concentration varies depending on the copy number of target present in the template solution. Optimal amount should be determined by preparing the dilution series. DNA template should be used in less than 100 ng for 20 μl reaction. When the RT reactant (cdna) is used as a template, it should be added in less 10% volume of PCR reaction mixture. * 5 : The reaction of 50 μl is for 96-well plate, single tube and 8-strip tube. The reaction of 20 μl is for 384-well plate and 96-well Fast Themal Cycling Plate. 2. Start the reaction. Shuttle PCR standard protocol (below) is recommended. Try this protocol firstly, and optimize the reaction condition if needed. Note : This product combines the high performance of TaKaRa Ex Taq HS, which is an enzyme for hot start PCR utilizing Taq antibody. Initial denaturation step prior to PCR should be at 95 for 30 sec. No need to heat at 95 for (5 - ) 15 min. as the initial denaturation, required for chemically modified Taq polymerase. If excessive heat treatment is provided, the enzyme activity descreases and the amplification efficiency and the accuracy in quantification can also be affected. 7

8 [ABI PRISM 7000/7700/7900HT, 7300/7500 Real-Time PCR System] Shuttle PCR Standard Protocol Stage 1 : Initial denaturation Reps : sec. Stage 2 : PCR Reps : sec sec. (31sec, 34sec) * * : Fluorescence detection step should be 30 seconds with ABI PRISM 7700 and 7900HT, 31 seconds with ABI PRISM 7000 and 7300 Real-Time PCR System, and 34 seconds with 7500 Real-Time PCR System. [7500 Fast Real-Time PCR System] Shuttle PCR Standard Protocol Holding Stage Reps:1 95, 30 min. Cycling Stage Number of Cycles : 40 95, 3 min. 60, 30 min. Melt Curve Stage 8

9 Initial denaturation step Step Temperature Time Detection Remark Initial denaturation sec. OFF It is recommended to perform initial denaturation at 95 for 30 seconds, which is enough even when the template is genomic DNA or circular plasmid. Denaturation condition that exceeds 2 minutes unstabilizes the reaction thus not recommended. Shuttle PCR Step Temperature Time Detection Remark Denaturation sec. OFF Annealing/ Extension sec. (25, 30, 31, 34 sec.) * ON Since the target size amplified for real time PCR is generally shorter than 300 bp, the denaturation at 95 for 3-5 seconds is a sufficient condition. Please try at 60 for 30, 31, 34 or 25 seconds at first. The temperature should be optimized within the range of if optimization is required. When reaction does not proceed efficiently, extend the time. * : Fluorescence detection step should be 30 seconds with ABI PRISM 7700 and 7900HT, 31 seconds with 7000 and 7300, and 34 seconds with 7500 Real-Time PCR System. When used with 7500 Fast, set 25 sec. Cycle number;30-45 cycles 3. After the reaction is completed, verify the amplification curve. Establish the standard curve when quantification is done. * Refer to the operation manual of an used real time PCR instrument. C) Protocol using LightCycler 1. Prepare the following PCR reaction mixture. <per reaction> [ Reagents ] [ Volume ] [ Final concentration ] Premix Ex Taq (2 ) 10 μl 1 PCR Forward Primer (10 μm) 0.4 μl 0.2 μm *1 PCR Reverse Primer (10 μm) 0.4 μl 0.2 μm *1 TaqMan probe 0.8 μl *2 template 2 μl *3 dh2o 6.4 μl total 20 μl 9

10 * 1 : The final concentration of primers can be 0.2 μm in most reactions. When it does not work, determine the optimalconcentrations within the range μm. * 2 : Probe concentration differs depending on kind of real time PCR instrument, and kind of fluorescence labeling material. The adding amount should be determined by referring to the operation manual of instrument and a product insert supplied with probe. * 3 : Final template concentration varies depending on the copy number of target present in the template solution. Optimal amount should be determined by preparing the dilution series. DNA template should be used in less than 100 ng. When the RT reactant (cdna) is used as a template, it should be added in less 10% volume of PCR reaction mixture. 2. Start the reaction. After gently centrifugation of LightCycler capillaries, set capillaries in a LightCycler and start the reaction. Shuttle PCR standard protocol (below) is recommended. Try this protocol firstly, and optimize the reaction condition if needed. Note : This product combines the high performance of TaKaRa Ex Taq HS, which is an enzyme for hot start PCR utilizing Taq antibody. Initial denaturation step prior to PCR should be at 95 for 30 sec. No need to heat at 95 for (5 -) 15 min. as the initial denaturation, required for chemically modified Taq polymerase. If excessive heat treatment is provided, the enzyme activity descreases and theamplification efficiency and the accuracy in quantification can also be affected. Stage2 : PCR reaction Shuttle PCR Standard Protocol Stage 1 : Initial denaturation Reps : 1 95, 30 sec. 20 /sec. Stage 2 : PCR Reps : 40 95, 5 sec. 20 /sec. 60, 20 sec. 20 /sec. 10

11 Initial denaturation Step Temperature Time Acquisition Mode Initial denaturation sec. None Remark It is recommended to perform initial denaturation at 95 for 30 seconds, which is enough even when the template is genomic DNA or circular plasmid. Denaturation condition that exceeds 2 minutes unstabilizes the reaction thus not recommended. Shuttle PCR Step Temperature Time Acquisition Mode Denaturation sec. None Annealing /Extension sec. Single Remark Since the target size amplified for real time PCR is generally shorter than 300 bp, the denaturation at 95 for 3-5 seconds is a sufficient condition. Please try at 60 for 20 seconds at first. The temperature should be optimized within the range of if optimization is required.when reaction does not proceed efficiently, extend the time. Cycle number : cycles 3. After the reaction is completed, verify the amplification curve. Establish the standard curve when quantification is done. * Refer to the operation manual of an used real time PCR instrument. 11

12 D) Protocol using Mx3000P 1. Prepare the following PCR reaction mixture on ice. <per reaction> [ Reagents ] [ Volume ] [ Final conc. ] Premix Ex Taq (2 ) 12.5 μl 1 PCR Forward Primer (10 μm) 0.5 μl 0.2 μm *1 PCR Reverse Primer (10 μm) 0.5 μl 0.2 μm *1 TaqMan probe 1 μl *2 ROX Reference Dye II (50 ) *3 0.5 μl 1 template (<100 ng) 2 μl *4 dh2o 8.0 μl total 25 μl * 1 : The final concentration of primers can be 0.2 μm in most reactions. When it does not work, determine the optimal concentrations within the range of μm. * 2 : Probe concentration differs depending on kind of real time PCR instrument and type of fluorescence labeling material. The adding amount should be determined by referring to the operation manual of instrument and a product insert supplied with probe. * 3 : For Mx3000P, the use of ROX Reference Dye II is recomended. ROX Reference Dye is not suitable for the use with Mx3000P, since it has higher concentration than ROX Reference Dye II. * 4 : Final template concentration varies depending on the copy number of target present in the template solution. Optimal amount should be determined by preparing the dilution series. It is recomended to apply DNA template in less than 100 ng. When the RT reactant (cdna) is used as a template, it should be added in less than 10% volume of PCR reaction mixture. 2. Start the reaction. Shuttle PCR standard protocol (below) is recommended. Try this protocol firstly, and optimize the reaction condition if needed. Shuttle PCR Standard Protocol Stage 1 : Initial denaturation Reps : 1 95, 30 sec. Stage 2 : PCR Reps : 40 95, 5 sec. 60, 20 sec. Note : This product combines the high performance of TaKaRa Ex Taq HS, which is an enzyme for hot start PCR utilizing Taq antibody. Initial denaturation step prior to PCR should be at 95 for 30 sec. No need to heat at 95 for (5 -) 15 min. as the initial denaturation, required for chemically modified Taq polymerase. If excessive heat treatment is provided, the enzyme activity descreases and theamplification efficiency and the accuracy in quantification can also be affected. 12

13 Initial denaturation Step Temperature Time Detection Remark Initial denaturation sec. OFF It is recommended to perform initial denaturation at 95 for 30 seconds, which is enough even when the template is genomic DNA or circular plasmid. Denaturation condition that exceeds 2 minutes unstabilizes the reaction thus not recommended. Shuttle PCR Step Temperature Time Detection Remark Denaturation sec. OFF Annealing /Extension sec. ON Since the target size amplified for real time PCR is generally shorter than 300 bp, the denaturation at 95 for 3-5 seconds is a sufficient condition. Please try at 60 for 20 seconds at first. The temperature should be optimized within the range of if optimization is required. When reaction does not proceed efficiently, extend the time. Cycle number : cycles 3. After the reaction is completed, perform analysis. After the reaction is completed, verify the amplification curve. Establish the standard curve when quatitation is done. * Refer to the operation manual of an used real time PCR instrument. 13

14 VIII. Application example Detection example with Applied Biosystems 7500 Fast Real-Time PCR System Target : Mouse Gapd Primer/Probe : TaqMan Gene Expression Assays (Life Technologies) Template : cdna serial dilution (corresponding to total RNA 500 fg - 50 ng) Amplification curve sec sec sec. 45 cycle Standard curve R 2 = Slope = IX. Related products PrimeScript RT reagent Kit (Perfect Real Time) (Cat. #RR037A) One Step PrimeScript RT-PCR Kit (Perfect Real Time) (Cat. #RR064A) SYBR Premix Ex Taq II (Tli RNaseH Plus) (Cat. #RR820A/B) SYBR Premix Ex Taq (Tli RNaseH Plus) (Cat. #RR420A/B) One Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time) (Cat. #RR086A) One Step SYBR PrimeScript RT-PCR Kit (Perfect Real Time) (Cat. #RR066A) 14

15 NOTICE TO PURCHASER: LIMITED LICENSE [P7] PCR Notice A license to perform the patented 5' Nuclease Process for research is obtained by the purchase of (i) both Authorized 5' Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5' Nuclease Kit, or (iii) license rights from Applied Biosystems. This product is an Authorized 5' Nuclease Core Kit. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,210,015, 5,487,972, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos. 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and 6,214,979, and corresponding claims outside the United States. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. [L15] Hot Start PCR Licensed under U.S. Patent No ,671 and 5,587,287, and corresponding patents in other countries. [L52] Rox Reference Dye (Research Field) Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. [M57] LA Technology This product is covered by the claims 6-16 of U.S. Patent No. 5,436,149 and its foreign counterpart patent claims. TaqMan The 5' nuclease process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. Purchase of the product does not provide a license to use this patented technology. 15

16 NOTE : This product is for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals. Also, do not use this product as food, cosmetic, or household item, etc. Takara products may not be resold or transferred, modified for resale or transfer, or used to manufacture commercial products without written approval from TAKARA BIO INC. If you require licenses for other use, please contact us by phone at or from our website at 16

Reference Dye N/A ROX & ROX II (as separate tubes) ROX & ROX II (as separate tubes)

Reference Dye N/A ROX & ROX II (as separate tubes) ROX & ROX II (as separate tubes) Comparison of TaKaRa s and qrt-pcr Kits SYBR Premix Ex Taq (Tli RNase H+) Premix Ex Taq (Probe qpcr) SYBR Premix Ex Taq II (Tli RNase H+) Cat.# RR420A RR390A RR820A Reactions/Kit 200 (50 µl reaction size)

More information

SYBR Premix Ex Taq (Tli RNaseH Plus)

SYBR Premix Ex Taq (Tli RNaseH Plus) Cat. # RR420A For Research Use SYBR Premix Ex Taq (Tli RNaseH Plus) Product Manual Table of Contents I. Description... 3 II. Principle... 3 III. Components... 4 IV. Materials Required but not Provided...

More information

PrimeScript RT Master Mix (Perfect Real Time) Sample

PrimeScript RT Master Mix (Perfect Real Time) Sample Cat. # RR036Q For Research Use PrimeScript RT Master Mix (Perfect Real Time) Sample Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Materials Required but not Provided...

More information

Realtime PCR Master Mix

Realtime PCR Master Mix Instruction manual Realtime PCR Master Mix 0810 F0923K Realtime PCR Master Mix Contents [1] Introduction [2] Components [3] Primer/Probe design [4] Detection [5] Specimens [6] Protocol 1. TaqMan assay

More information

SYBR Green Realtime PCR Master Mix -Plus-

SYBR Green Realtime PCR Master Mix -Plus- Instruction manual SYBR Green Realtime PCR Master Mix -Plus- 0810 F0925K SYBR Green Realtime PCR Master Mix -Plus- Contents QPK-212T 1mLx1 QPK-212 1mLx5 Store at -20 C, protected from light [1] Introduction

More information

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3

Table of Contents. I. Description... 2. II. Kit Components... 2. III. Storage... 2. IV. 1st Strand cdna Synthesis Reaction... 3 Table of Contents I. Description... 2 II. Kit Components... 2 III. Storage... 2 IV. 1st Strand cdna Synthesis Reaction... 3 V. RT-PCR, Real-time RT-PCR... 4 VI. Application... 5 VII. Preparation of RNA

More information

mircute mirna qpcr Detection Kit (SYBR Green)

mircute mirna qpcr Detection Kit (SYBR Green) mircute mirna qpcr Detection Kit (SYBR Green) For detection of mirna using real-time RT-PCR (SYBR Green I) www.tiangen.com QP110302 mircute mirna qpcr Detection Kit (SYBR Green) Kit Contents Cat. no. FP401

More information

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C

MystiCq microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C microrna cdna Synthesis Mix Catalog Number MIRRT Storage Temperature 20 C Product Description The microrna cdna Synthesis Mix has been designed to easily convert micrornas into cdna templates for qpcr

More information

PrimeSTAR HS DNA Polymerase

PrimeSTAR HS DNA Polymerase Cat. # R010A For Research Use PrimeSTAR HS DNA Polymerase Product Manual Table of Contents I. Description...3 II. III. IV. Components...3 Storage...3 Features...3 V. General Composition of PCR Reaction

More information

Blend Taq / Blend Taq -Plus-

Blend Taq / Blend Taq -Plus- Instruction manual Blend Taq and Blend Taq -Plus- 0810 F0938K Blend Taq / Blend Taq -Plus- BTQ-101 250 U 200 reactions BTQ-201 250 U 200 reactions Contents [1] Introduction

More information

Premium PCR Enzymes. High Fidelity PCR Real Time PCR RT- PCR qrt- PCR High-Speed PCR Long PCR Routine PCR Multiplex PCR Hot-Start PCR GC-Rich PCR

Premium PCR Enzymes. High Fidelity PCR Real Time PCR RT- PCR qrt- PCR High-Speed PCR Long PCR Routine PCR Multiplex PCR Hot-Start PCR GC-Rich PCR Premium PCR Enzymes Introducing SapphireAmp "Load'N'Go" fast PCR master mix... p 15 EmeraldAmp "Load'N'Go" PCR master mixes for superior performance on GC or AT rich regions...p 19 SYBR Premix Ex Taq (Tli

More information

Precision OneStep TM qrt-pcr Mastermix

Precision OneStep TM qrt-pcr Mastermix Precision OneStep TM qrt-pcr Mastermix Instructions for use of Primerdesign Precision OneStep TM Mastermix for real-time RT-PCR ONE OneStep MasterMix Contents Introduction 3 Kit Contents 5 Recommended

More information

PrecisionPLUS TM PrecisionFAST TM Precision TM Mastermix

PrecisionPLUS TM PrecisionFAST TM Precision TM Mastermix PrecisionPLUS TM PrecisionFAST TM Precision TM Mastermix Instructions for use of PrimerDesign Precision TM Mastermix/Precision Plus TM Precision TM FAST Mastermix Mastermix and MM MasterMix Contents Introduction

More information

Precision TM FAST and Precision TM Mastermix

Precision TM FAST and Precision TM Mastermix Precision TM FAST and Precision TM Mastermix Instructions for use of PrimerDesign Precision TM Mastermix and Precision TM FAST Mastermix MM MasterMix Contents Introduction 3 Kit Contents 5 Recommended

More information

PicoMaxx High Fidelity PCR System

PicoMaxx High Fidelity PCR System PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision B Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED

More information

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr

Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories,

More information

Introduction To Real Time Quantitative PCR (qpcr)

Introduction To Real Time Quantitative PCR (qpcr) Introduction To Real Time Quantitative PCR (qpcr) SABiosciences, A QIAGEN Company www.sabiosciences.com The Seminar Topics The advantages of qpcr versus conventional PCR Work flow & applications Factors

More information

BacReady TM Multiplex PCR System

BacReady TM Multiplex PCR System BacReady TM Multiplex PCR System Technical Manual No. 0191 Version 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI Detailed Experimental

More information

PicoMaxx High Fidelity PCR System

PicoMaxx High Fidelity PCR System PicoMaxx High Fidelity PCR System Instruction Manual Catalog #600420 (100 U), #600422 (500 U), and #600424 (1000 U) Revision C Research Use Only. Not for Use in Diagnostic Procedures. 600420-12 LIMITED

More information

Real-Time PCR Vs. Traditional PCR

Real-Time PCR Vs. Traditional PCR Real-Time PCR Vs. Traditional PCR Description This tutorial will discuss the evolution of traditional PCR methods towards the use of Real-Time chemistry and instrumentation for accurate quantitation. Objectives

More information

Essentials of Real Time PCR. About Sequence Detection Chemistries

Essentials of Real Time PCR. About Sequence Detection Chemistries Essentials of Real Time PCR About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is therefore collected

More information

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE.

FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE. Instruction manual KOD -Plus- 0902 F0934K KOD -Plus- Contents [1] Introduction [2] Components [3] Quality testing [4] Primer design [5] Cloning of PCR products [6] Protocol 1. Standard reaction setup 2.

More information

Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR

Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR Protocol Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR Copyright 2008, 2010 Applied Biosystems. All rights reserved. Ambion and Applied Biosystems products are for Research Use Only.

More information

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit

Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit Product Bulletin Human Identification Quantifiler Human DNA Quantification Kit Quantifiler Y Human Male DNA Quantification Kit The Quantifiler kits produce reliable and reproducible results, helping to

More information

4 General PCR Methods Page

4 General PCR Methods Page Table of Contents General PCR Methods Page PCR Protocol Selection Guide...65.1 Basic PCR... 66.1.1 Hot Start PCR - The new Standard... 66.1.1.1 Reagents and Equipment Required... 66.1.1.2 General Considerations

More information

Super One-step RT-PCR Kit for Virus Detection

Super One-step RT-PCR Kit for Virus Detection Super One-step RT-PCR Kit for Virus Detection For fast and sensitive one-step RT-PCR with highly specificity www.tiangen.com/en SD121221 Super One-step RT-PCR Kit Kit Contents Contents Cat. no. SD103 SD103

More information

TaqMan Fast Advanced Master Mix. Protocol

TaqMan Fast Advanced Master Mix. Protocol TaqMan Fast Advanced Master Mix Protocol For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. Information in this document is subject to change without notice. APPLIED

More information

Highly specific and sensitive quantitation

Highly specific and sensitive quantitation PRODUCT ULLETIN SYR Select Master Mix SYR Select Master Mix Highly specific and sensitive quantitation SYR Select Master Mix offers advanced performance at an affordable price. SYR Select Master Mix is

More information

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Epstein Barr Virus (Human Herpes virus 4) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests

Dengue Virus subtypes 1,2 3 and 4. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... 3 Untranslated Region (3 UTR) 150 tests TM Primerdesign Ltd TM Primerdesign Ltd Dengue Virus subtypes 1,2 3 and 4 3 Untranslated Region (3 UTR) genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory

More information

Terra PCR Direct Polymerase Mix User Manual

Terra PCR Direct Polymerase Mix User Manual Clontech Laboratories, Inc. Terra PCR Direct Polymerase Mix User Manual Cat. Nos. 639269, 639270, 639271 PT5126-1 (031416) Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain

More information

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere...

Human Herpes Virus 1 (Herpes simplex type 1) genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... TM Primerdesign Ltd TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

Real-time quantitative RT -PCR (Taqman)

Real-time quantitative RT -PCR (Taqman) Real-time quantitative RT -PCR (Taqman) Author: SC, Patti Lab, 3/03 This is performed as a 2-step reaction: 1. cdna synthesis from DNase 1-treated total RNA 2. PCR 1. cdna synthesis (Advantage RT-for-PCR

More information

An introduction to PCR and real-time PCR Dr. F. Waldherr CONGEN Biotechnologie GmbH

An introduction to PCR and real-time PCR Dr. F. Waldherr CONGEN Biotechnologie GmbH An introduction to PCR and real-time PCR Dr. F. Waldherr CONGEN Biotechnologie GmbH DNA basic principles DNA carries the information of life on Earth DNA is present in all organisms (some viruses use RNA)

More information

Stratagene QPCR Mouse Reference Total RNA

Stratagene QPCR Mouse Reference Total RNA Stratagene QPCR Mouse Reference Total RNA Instruction Manual Catalog #750600 Revision C.0 For Research Use Only. Not for use in diagnostic procedures. 750600-12 LIMITED PRODUCT WARRANTY This warranty limits

More information

Quantification of Mycobacterium Tuberculosis. 150 tests

Quantification of Mycobacterium Tuberculosis. 150 tests Quantification of Mycobacterium Tuberculosis rep 13E12 For general laboratory and research use only 150 tests Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis is the bacterium that

More information

Validating Microarray Data Using RT 2 Real-Time PCR Products

Validating Microarray Data Using RT 2 Real-Time PCR Products Validating Microarray Data Using RT 2 Real-Time PCR Products Introduction: Real-time PCR monitors the amount of amplicon as the reaction occurs. Usually, the amount of product is directly related to the

More information

E.Z.N.A. Plant Seed Direct PCR Kit

E.Z.N.A. Plant Seed Direct PCR Kit E.Z.N.A. Plant Seed Direct PCR Kit TQ2900-00 TQ2900-01 20 preps 100 preps June 2013 E.Z.N.A. Plant Seed Direct PCR Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Suggested

More information

Human Herpes Virus 4 (Epstein Barr)

Human Herpes Virus 4 (Epstein Barr) Techne qpcr test Human Herpes Virus 4 (Epstein Barr) nonglycosylated membrane protein (BNRF1) gene 150 tests For general laboratory and research use only 1 Introduction to Human Herpes Virus 4 (Epstein

More information

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing

Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene. genesig Advanced Kit. DNA testing TM Primerdesign Ltd TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere...

More information

GenScript BloodReady TM Multiplex PCR System

GenScript BloodReady TM Multiplex PCR System GenScript BloodReady TM Multiplex PCR System Technical Manual No. 0174 Version 20040915 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 2 V Simplified Procedures. 2 VI

More information

Factors Influencing Multiplex Real-Time PCR

Factors Influencing Multiplex Real-Time PCR APPLICATION NOTE Multiplex Real-Time PCR Factors Influencing Multiplex Real-Time PCR Introduction Multiplex PCR is the simultaneous amplification of more than one target sequence in a single reaction [1].

More information

RT-PCR: Two-Step Protocol

RT-PCR: Two-Step Protocol RT-PCR: Two-Step Protocol We will provide both one-step and two-step protocols for RT-PCR. We recommend the twostep protocol for this class. In the one-step protocol, the components of RT and PCR are mixed

More information

oasig TM lyophilised 2X qpcr Mastermix

oasig TM lyophilised 2X qpcr Mastermix oasig TM lyophilised 2X qpcr Mastermix Instructions for use of Primerdesign oasig lyophilised Mastermix MM MasterMix Contents Introduction 3 Kit Contents 4 Kit Storage 4 Suitable Sample Material 4 Licensing

More information

RealStar HBV PCR Kit 1.0 11/2012

RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße

More information

Application Guide... 2

Application Guide... 2 Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue Application Guide... 2 I. Description... 2 II. Product Components... 2 III. Materials to be Supplied

More information

Quantitative Real Time PCR Protocol. Stack Lab

Quantitative Real Time PCR Protocol. Stack Lab Quantitative Real Time PCR Protocol Stack Lab Overview Real-time quantitative polymerase chain reaction (qpcr) differs from regular PCR by including in the reaction fluorescent reporter molecules that

More information

qstar mirna qpcr Detection System

qstar mirna qpcr Detection System qstar mirna qpcr Detection System Table of Contents Table of Contents...1 Package Contents and Storage Conditions...2 For mirna cdna synthesis kit...2 For qstar mirna primer pairs...2 For qstar mirna qpcr

More information

TECHNICAL BULLETIN. REDExtract-N-Amp Tissue PCR Kit. Catalog Numbers XNATS, XNAT, XNATR

TECHNICAL BULLETIN. REDExtract-N-Amp Tissue PCR Kit. Catalog Numbers XNATS, XNAT, XNATR REDExtract-N-Amp Tissue PCR Kit Catalog Numbers XNATS, XNAT, XNATR TECHNICAL BULLETIN Product Description The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify

More information

Mir-X mirna First-Strand Synthesis Kit User Manual

Mir-X mirna First-Strand Synthesis Kit User Manual User Manual Mir-X mirna First-Strand Synthesis Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc.

More information

TECHNICAL BULLETIN. REDExtract-N-Amp Tissue PCR Kit. Catalog Numbers XNAT-10RXN, XNAT-100RXN, XNAT-1000RXN

TECHNICAL BULLETIN. REDExtract-N-Amp Tissue PCR Kit. Catalog Numbers XNAT-10RXN, XNAT-100RXN, XNAT-1000RXN REDExtract-N-Amp Tissue PCR Kit Catalog Numbers XNAT-10RXN, XNAT-100RXN, XNAT-1000RXN TECHNICAL BULLETIN Product Description The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly

More information

amplification tech Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye

amplification tech Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye amplification tech note 6047 Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye Liz Jordan and Richard Kurtz, Gene Expression Division, io-rad

More information

PrimeScript High Fidelity RT-PCR Kit

PrimeScript High Fidelity RT-PCR Kit For Research Use PrimeScript High Fidelity RT-PCR Kit Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Materials Required but not Provided... 4 IV. Storage... 4 V. Fidelity

More information

KAPA Library Quantification Kits For Illumina sequencing platforms

KAPA Library Quantification Kits For Illumina sequencing platforms Technical Data Sheet KAPA Library Quantification Kits For Illumina sequencing platforms Kit code KK4824 KK4601: Components KAPA SYBR FAST Universal qpcr Kit 1. Product Description Accurate quantification

More information

miracle mirna qpcr Instruction Manual Store at -20 Version 3.0 (Aug.2013)

miracle mirna qpcr Instruction Manual Store at -20 Version 3.0 (Aug.2013) miracle mirna qpcr Instruction Manual Store at -20 Version 3.0 (Aug.2013) Contents I. PRODUCT INTRODUCTION 1 A. OVERVIEW 1 B. KIT CONTENTS 1 1. REAGENT KITS 1 2. PRIMER SETS 3 3. MIRACLE ON CALL SCREEN

More information

Fundamentals of Real-Time PCR. Poupak Farahani, Ph.D. Senior Product Specialist

Fundamentals of Real-Time PCR. Poupak Farahani, Ph.D. Senior Product Specialist Fundamentals of Real-Time PCR Poupak Farahani, Ph.D. Senior Product Specialist Polymerase Chain Reaction (PCR) Unamplified DNA Strand Denaturation Primer annealing PCR Primer extension Dreischrittreaktion

More information

Quantitative Telomerase Detection Kit (QTD Kit)

Quantitative Telomerase Detection Kit (QTD Kit) Quantitative Telomerase Detection Kit (QTD Kit) Catalog No. MT3010, MT3011, MT3012 For Research Use Only. Not for use in diagnostic procedures 1 Table of Contents 1. Introduction Background Product Overview

More information

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd.

Lyme Disease. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... RecA gene. 150 tests. Primerdesign Ltd. TM Primerdesign Ltd TM Primerdesign Ltd Lyme Disease RecA gene genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction

More information

quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476

quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476 BioScience quantitative real-time PCR, grain, simplex DNA extraction: PGS0426 RT-PCR: PGS0494 & PGS0476 This method describes a Real-time semi-quantitative TaqMan PCR procedure for the determination of

More information

Idaho Technology Inc. Kit No. HRLS-ASY Reactions Kit No. HRLS-ASY Reactions

Idaho Technology Inc.  Kit No. HRLS-ASY Reactions Kit No. HRLS-ASY Reactions Idaho Technology Inc. www.idahotech.com Kit No. HRLS-ASY-0002 100 Reactions Kit No. HRLS-ASY-0003 500 Reactions Kit Contents Quantity 1 x 100 Reactions OR 5 x 100 Reactions Description 2.5X Master Mix

More information

HBV Quantitative Real Time PCR Kit

HBV Quantitative Real Time PCR Kit Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative Real Time PCR Kit Cat. No.: HD-0002-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems (Pls ignore

More information

All-in-One mirna qrt-pcr Detection System Handbook

All-in-One mirna qrt-pcr Detection System Handbook All-in-One mirna qrt-pcr Detection System Handbook For quantitative detection of mature mirna All-in-One mirna First-Strand cdna Synthesis Kit Cat. No. AMRT-0020 (20 mirna reverse transcription reactions)

More information

Speed Matters - Fast ways from template to result

Speed Matters - Fast ways from template to result qpcr Symposium 2007 - Weihenstephan Speed Matters - Fast ways from template to result March 28, 2007 Dr. Thorsten Traeger Senior Scientist, Research and Development - 1 - Overview Ạgenda Fast PCR The Challenges

More information

TECHNICAL BULLETIN. REDExtract-N-Amp Seed PCR Kit. Catalog Numbers XNASS, XNAS, and XNASR

TECHNICAL BULLETIN. REDExtract-N-Amp Seed PCR Kit. Catalog Numbers XNASS, XNAS, and XNASR REDExtract-N-Amp Seed PCR Kit Catalog Numbers XNASS, XNAS, and XNASR TECHNICAL BULLETIN Product Description The REDExtract-N-Amp Seed PCR Kit contains all the reagents needed to rapidly extract and amplify

More information

DyNAmo Flash Probe qpcr Kit DyNAmo ColorFlash Probe qpcr Kit

DyNAmo Flash Probe qpcr Kit DyNAmo ColorFlash Probe qpcr Kit DyNAmo Flash Probe qpcr Kit DyNAmo ColorFlash Probe qpcr Kit Now sold as Thermo Scientific F-455 F-456 Now sold as Thermo Scientific Instruction manual DyNAmo Flash Probe qpcr Kit F-455S Sufficient for

More information

Absolute Quantification Getting Started Guide

Absolute Quantification Getting Started Guide 5 cdna Reverse Primer Oligo d(t) or random hexamer Synthesis of 1st cdna strand 3 5 cdna Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Absolute Quantification Getting Started Guide Introduction

More information

GoTaq 2-Step RT-qPCR System

GoTaq 2-Step RT-qPCR System TECHNICAL MANUAL GoTaq 2-Step RT-qPCR System Instruc ons for use of Product A6010 Revised 1/14 TM337 GoTaq 2-Step RT-qPCR System All technical literature is available on the Internet at: www.promega.com/protocols/

More information

Path-ID Multiplex One-Step RT-PCR Kit

Path-ID Multiplex One-Step RT-PCR Kit USER GUIDE Path-ID Multiplex One-Step RT-PCR Kit TaqMan probe-based multiplex one-step real-time RT-PCR detection of RNA targets Catalog Numbers 4428206, 4428207, 4440022 Publication Part Number 4440907

More information

Brilliant II QRT-PCR Master Mix with ROX, 1-Step

Brilliant II QRT-PCR Master Mix with ROX, 1-Step Brilliant II QRT-PCR Master Mix with ROX, 1-Step Instruction Manual Catalog #600838 (Brilliant II QRT-PCR High ROX Master Mix, 1-Step) #600837 (Brilliant II QRT-PCR Low ROX Master Mix, 1-Step) #600842

More information

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube

User Manual. CelluLyser Lysis and cdna Synthesis Kit. Version 1.4 Oct 2012 From cells to cdna in one tube User Manual CelluLyser Lysis and cdna Synthesis Kit Version 1.4 Oct 2012 From cells to cdna in one tube CelluLyser Lysis and cdna Synthesis Kit Table of contents Introduction 4 Contents 5 Storage 5 Additionally

More information

Put the odds in your favor

Put the odds in your favor Reverse Transcription Put the odds in your favor SuperScript III Reverse Transcriptase Reverse Transcription Engineered for performance SuperScript III Reverse Transcriptase (RT) is engineered for reduced

More information

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna

All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One mirna qrt-pcr Reagent Kits For quantitative detection of mature mirna All-in-One TM mirna First-Strand cdna Synthesis Kit AMRT-0020 (20 RT reactions), AMRT-0060 (60 RT reactions) Used in combination

More information

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests.

Hepatitis B Virus. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Core Protein Region. 150 tests. TM Primerdesign Ltd TM Primerdesign Ltd Hepatitis B Virus Core Protein Region genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use

More information

All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources

All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources All in One First Strand cdna Synthesis Kit For reliable first strand cdna synthesis from all RNA sources Cat. No. AORT 0020 Cat. No. AORT 0050 (20 synthesis reactions) (50 synthesis reactions) User Manual

More information

Technical Note. Roche Applied Science. No. LC 18/2004. Assay Formats for Use in Real-Time PCR

Technical Note. Roche Applied Science. No. LC 18/2004. Assay Formats for Use in Real-Time PCR Roche Applied Science Technical Note No. LC 18/2004 Purpose of this Note Assay Formats for Use in Real-Time PCR The LightCycler Instrument uses several detection channels to monitor the amplification of

More information

Gene Expression Assays

Gene Expression Assays APPLICATION NOTE TaqMan Gene Expression Assays A mpl i fic ationef ficienc yof TaqMan Gene Expression Assays Assays tested extensively for qpcr efficiency Key factors that affect efficiency Efficiency

More information

QPCR Applications using Stratagene s Mx Real-Time PCR Platform

QPCR Applications using Stratagene s Mx Real-Time PCR Platform QPCR Applications using Stratagene s Mx Real-Time PCR Platform Dan Schoeffner, Ph.D Field Applications Scientist Dan.Schoeffner@Stratagene.com Tech. Services 800-894-1304 Polymerase Chain Reaction Melt

More information

Troubleshooting Sequencing Data

Troubleshooting Sequencing Data Troubleshooting Sequencing Data Troubleshooting Sequencing Data No recognizable sequence (see page 7-10) Insufficient Quantitate the DNA. Increase the amount of DNA in the sequencing reactions. See page

More information

Optimizing Real Time PCR: A Focused Approach for Exceptional Real-Time qpcr Results

Optimizing Real Time PCR: A Focused Approach for Exceptional Real-Time qpcr Results Optimizing Real Time PCR: A Focused Approach for Exceptional Real-Time qpcr Results Michael Wakem M.Sc Bio-Rad Laboratories Canada Ltd (800) 268-0213 Ext 3360 michael_wakem@bio-rad.com Overview Fundamentals

More information

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests

Quantification of Hepatitis B Virus. Core Protein Region. 150 tests Quantification of Hepatitis B Virus Core Protein Region For general laboratory and research use only 150 tests Introduction to Hepatitis B Virus Originally known as serum hepatitis, hepatitis B has only

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit

Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit USER GUIDE Power SYBR Green PCR Master Mix and Power SYBR Green RT-PCR Reagents Kit Catalog Number 4368577, 4367659, 4367660, 4368706, 4368702, 4368708 (Master Mix) and 4368711 (RT-PCR Reagents Kit) Publication

More information

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit

SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit USER GUIDE SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit Catalog Number 4309155 (Master Mix) and 4306736 (RT-PCR Reagents Kit) Publication Part Number 4310251 Rev. G Revision Date September

More information

Genomic DNA detection assay

Genomic DNA detection assay Genomic DNA detection assay Detection of genomic DNA by real-time PCR Contents CTRL Internal controls and gdna detection Contents Kit Contents 3 Reagents and Equipment to Be Supplied by User 3 Kit Storage

More information

Technical Manual No. 0173 Update Date 10112010

Technical Manual No. 0173 Update Date 10112010 TissueDirect TM Multiplex PCR System Technical Manual No. 0173 Update Date 10112010 I Description.. 1 II Applications 2 III Key Features.. 2 IV Shipping and Storage. 3 V Simplified Procedures. 3 VI Detailed

More information

Legionella pneumophila Detection Kit (Real-Time)

Legionella pneumophila Detection Kit (Real-Time) Unzipping Genes P r o d u c t I n f o r m a t i o n MBPCR028 Legionella pneumophila Detection Kit (Real-Time) Description: Legionella pneumophila is a gram-negative, non-encapsulated, aerobic bacillus

More information

VeriFiler Direct PCR Amplification Kit

VeriFiler Direct PCR Amplification Kit QUICK REFERENCE VeriFiler Direct PCR Amplification Kit Publication Number 4479554 Rev. A Note: For safety and biohazard guidelines, refer to the Safety section in the VeriFiler Direct PCR Amplification

More information

Lyme Disease. RecA gene. 150 tests. Quantification of Lyme Disease genomes. Advanced kit handbook HB10.03.07

Lyme Disease. RecA gene. 150 tests. Quantification of Lyme Disease genomes. Advanced kit handbook HB10.03.07 Techne qpcr test Lyme Disease RecA gene 150 tests For general laboratory and research use only 1 Introduction to Lyme Disease Lyme disease is an infectious disease caused mainly by three species of bacteria

More information

Lyme Disease RecA gene. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... 150 tests. Primerdesign Ltd.

Lyme Disease RecA gene. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... 150 tests. Primerdesign Ltd. TM Primerdesign Ltd TM Primerdesign Ltd Lyme Disease RecA gene genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction

More information

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual

Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual Thermo Scientific DyNAmo cdna Synthesis Kit for qrt-pcr Technical Manual F- 470S 20 cdna synthesis reactions (20 µl each) F- 470L 100 cdna synthesis reactions (20 µl each) Table of contents 1. Description...

More information

Reagents for PCR. Expertise in Amplification

Reagents for PCR. Expertise in Amplification Reagents for PCR Expertise in Amplification Selection Guide Hot Start Enzymes for Specific Requirements PCR Application: Routine PCR Fast PCR Hot Start Multiplex/ Sequencing Wide Variety/ Easy Access High

More information

Sequencing Library qpcr Quantification Guide

Sequencing Library qpcr Quantification Guide Sequencing Library qpcr Quantification Guide FOR RESEARCH USE ONLY Introduction 3 Quantification Workflow 4 Best Practices 5 Consumables and Equipment 6 Select Control Template 8 Dilute qpcr Control Template

More information

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-)

PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) PyroPhage 3173 DNA Polymerase, Exonuclease Minus (Exo-) FOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE Lucigen Corporation 2905 Parmenter St, Middleton, WI 53562 USA Toll Free: (888) 575-9695 (608)

More information

EPIK mirna Select Hi/Lo-ROX Kit. Product Manual

EPIK mirna Select Hi/Lo-ROX Kit. Product Manual EPIK mirna Select Hi/Lo-ROX Kit Product Manual 2 Product Manual www.bioline.com/mirna EPIK mirna Select Hi/Lo-ROX Kit EPIK mirna Select Kit TABLE OF CONTENTS 1 Kit contents 04 2 Description 04 3 Storage

More information

Borrelia burgdorferi

Borrelia burgdorferi Techne qpcr test Borrelia burgdorferi Outer surface protein A (ospa) gene 150 tests For general laboratory and research use only 1 Introduction to Borrelia burgdorferi Borrelia burgdorferi is a Gram negative

More information

PCR and qpcr solutions

PCR and qpcr solutions A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C G A T A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C A T C C C G C A T C C C G C A T C C C G C A T C C C G C A T C C G C

More information

Real Time PCR Kit for Human Herpes Virus Type 6 Cat No. TM-60027: Primer Set

Real Time PCR Kit for Human Herpes Virus Type 6 Cat No. TM-60027: Primer Set 780 Dubuque Avenue So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296 / Fax:(650)871-2857 http://www.maximbio.com E-mail: mbi@maximbio.com Real Time PCR Kit for Human Herpes Virus Type 6 Cat No. TM-60027:

More information

The AB7900Fast and Principles of Real Time PCR

The AB7900Fast and Principles of Real Time PCR The AB7900Fast and Principles of Real Time PCR Michael Tavaria, PhD Scientific Applications Specialist, Applied Biosystems, Australia and New Zealand Outline Chemistries 7900Fast Real Time PCR System Applications

More information

BioBank TM cdna Kit. Instructions for the use of BioBank TM cdna in real-time PCR. BB BioBank control cdna

BioBank TM cdna Kit. Instructions for the use of BioBank TM cdna in real-time PCR. BB BioBank control cdna BioBank TM cdna Kit Instructions for the use of BioBank TM cdna in real-time PCR BB BioBank control cdna Contents Introduction 3 Kit Contents 4 Reagents and Equipment to Be Supplied by User 4 PrimerDesign

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information