Universal qpcr kits for SYBR & Probe Assays

Transcription

1 Universal qpcr kits for SYBR & Probe Assays

2 Inspired by the faster than light particle (Tachyon), the Takyon TM qpcr line from Eurogentec is designed to give you access to a new level of speed in your qpcr applications. Emerging from the combination of an optimized reaction buffer and a new efficient fusion polymerase, Takyon TM kits for SYBR and Probe Assays ensure sensitivity and fast delivery of accurate and reproducible results! Universal capabilities The Takyon kits are compatible with all cyclers and applications up to automation assays. Large dynamic range The Takyon kits give a consistent amplification results over a large range of initial target concentrations Robust formulation The fusion polymerase is highly efficient and less sensitive to inhibitors. This allows efficient amplification on difficult templates like e.g. biological fluids and viruses. Efficient in small reaction volumes: The high sensitivity of the Takyon kits combined to the new highly concentrated 5X format give consistent results even in small volume reaction. Suitable for multiplex The sensitivity and efficiency of the Takyon kits allows the detection of multiple targets in a single reaction tube. Adapted to HTP automation With their optimized composition of the reaction buffer, the low viscosity HTP Takyon kits are perfectly suited for assay set-up automation. Custom formulation Our Takyon kits are more than catalog reagents. Please contact us to develop your own formulation tailored to your needs. NEW 5x Format #

3 Universal qpcr kits for SYBR & Probe Assays Takyon TM qpcr kit(s) for every needs Technology Formats SYBR or Probe Options MasterMix k Optimized ready-to-use mix k Maximal reproducibility k Reduced contamination risk Reaction Mix contains e.g.: Reaction buffer Takyon enzyme MgCl dntps Core Kit k Components in separated vials k Maximal flexibility k Customizable k Cost effective Kit contains x Buffer tube Takyon enzyme 5 mm MgCl 5mM dntp Mix UNG/dUTP blend VISUALIZE Combined with our inert BLUE Dye, Takyon qpcr Kits reveal to the naked eye dispensing errors that may occur during assay set-up. STANDARDIZE ROX passive reference Dye for well-to-well normalization of the recorded fluorescent signal. PREVENT Uracyl N-Glycosylase (UNG) is used to prevent DNA carryover contamination and cleave contaminating amplicons before amplification. CONVERT The Takyon TM One-Step kit converter can easily convert any Takyon TM qpcr dttp MasterMix into a One-Step qpcr kit. MAXIMIZE Maximize the template volume thanks to the highly concentrated new 5X MasterMix format. AUTOMATE The Low viscosity of the Takyon HTP 5X MasterMix allows more convenient assay set-up automation. NEW 5x Format

4 , Results k FAST SAVE TIME AND GET RESULTS FASTER Takyon kits achieve amplification with a very short extension time of 5 sec. k Figure : of a mitochondrial target (5 bp) was performed in duplicate using a 5 µl reaction mix containing Takyon No Rox Probe MasterMix BLUE dttp (UF-NPMT-B7) on a Roche LC 8 Cycler. Serial dilution (/8) starting from ng of human DNA (Sigma, D7) + NTC were tested in annealing-extension of: 5 sec., 5 sec. and sec. Recommended Protocol Takyon enzyme activation min 95 C Denaturation sec 95 C Annealing-Extension sec 6 C Takyon TM qpcr kits are also compatible with Regular Cyclers k ROBUST & SENSITIVE DETECT LOW COPY TARGETS WITH A BRIGHTER SIGNAL! Achieve outstanding sensitivity in your qpcr Assays with Takyon kits. Detect both low copy targets and product derived from difficult templates. 5 5 RFU 5 RFU l Eurogentec 5 l Competitor L k Figure : Comparison between Takyon Low ROX Probe MasterMix UNG (UF-LPMU-C7) and Competitor L. Experiment was performed in triplicate on a serial dilution /8, down to copies using the same primers, probe and cdna. BM (8 bp) amplification was monitored on a ABI Prism 75 Cycler ( min. activation 5 cycles with sec. denaturation and 5 sec. annealing-extension). #

5 k REPRODUCIBLE Universal qpcr kits for SYBR & Probe Assays CONSISTENT RESULTS EVERYTIME! Compatible with all cyclers and ready for high-throughput platforms, Takyon kits reduce variability and provide consistent reproducibility across different runs of the same template. RFU Cq 5 5 Log Starting Quantity l Eurogentec 5 Standard Curve E = 98 % R =,998 6 Log Starting Quantity 5 Standard Curve E = 98 % R =,998 6 Cq RFU RFU Standard Curve E = 86,5 % R =, Log Starting Quantity l Competitor Q k Figure : Takyon Probe MasterMixes ensure reproducibility. Comparison between Takyon No Rox Probe MasterMix dttp (UF-NPMT-C7) and Competitor Q. Experiment was performed in triplicate (7 serial dilutions /8 + NTC) using 5 µl reaction mix containing the same primers, probe and cdna; 5 pg cdnahu-ge, PrimerDesign. 8s (7 pb) amplification was monitored on a Bio-Rad CFX8 platform ( min. activation 5 cycles with sec. denaturation and sec. annealingextension). k SPECIFIC OBTAIN RESULTS WITHOUT ARTIFACTS Thanks to their optimized formulation, Takyon kits for SYBR ensure high specificity, easily assessed by melting curve analysis. A Melt Peak B RFU d(rfu)/dt Temperature, Celsius k Figure : A Real-time qpcr of 8-fold serial dilution of an eukaryotic elongation factor gene fragment (8 bp) was performed in triplicate using a 5 µl reaction Mix containing Takyon No Rox SYBR MasterMix Blue dttp (UF-NSMT-B7). The assay was performed on a Bio-Rad CFX96 platform ( min. activation 5 cycles with sec. denaturation and sec. extension). B Corresponding melt curve.

6 , Catalogue number Cycler Groups ABI Prism 7, 7, 77, 79, StepOne /Plus ABI Prism 75, Mx, MxP, Mx5P, QuantStudio TM K and ViiA 7 AriaMx, CFX96 & CFX 8, Chromo, DNA Engine Opticon &, icycler iq, iq 5, LC96, LC8, LightCycler Nano, MasterCycler ep realplex I & II, MiniOpticon, Mx5P, Mx, MxP, My iq, Smartcycler &, Quantica and Swift Spectrum 96, Rotor-Gene / /6 /Q icycler iq, My iq, iq 5 Free sample available Takyon TM for Probe Assays Cat, # Description µl RXNs ROX UF-RPMT-B UF-RPMT-B7 UF-RPMT-C UF-RPMT-C7 UF-RPMU-C UF-RPMU-C7 Takyon ROX Probe X MasterMix BLUE dttp Takyon ROX Probe X MasterMix dttp Takyon ROX Probe X MasterMix UNG All kits are available in 5 or packs formats UF-RP5X-C5 UF-RP5X-C Takyon ROX Probe 5X MasterMix dttp 5 RXN (,6 ml) 5 RXN (5 x ml) UF-RPCT-C Takyon ROX Probe X Core Kit dttp 5 RXN qpcr kits Low ROX No ROX ROX UF-LPMT-B Takyon Low ROX Probe X MasterMix BLUE dttp UF-LPMT-B7 UF-LPMT-C Takyon Low ROX Probe X MasterMix dttp UF-LPMT-C7 UF-LPMU-C Takyon Low ROX Probe X MasterMix UNG UF-LPMU-C7 UF-LP5X-C5 5 RXN (,6 ml) Takyon Low ROX Probe 5X MasterMix dttp UF-LP5X-C 5 RXN (5 x ml) UF-NPMT-B Takyon No ROX Probe X MasterMix BLUE dttp UF-NPMT-B7 UF-NPMT-C Takyon No ROX Probe X MasterMix dttp UF-NPMT-C7 UF-NPMU-C Takyon No ROX Probe X MasterMix UNG UF-NPMU-C7 UF-NPCT-C Takyon No ROX Probe X Core Kit dttp 5 RXN UF-NP5X-C5 5 RXN (,6 ml) Takyon No ROX Probe 5X MasterMix dttp UF-NP5X-C 5 RXN (5 x ml) UF-NP5X-CG5 5 RXN (,6 ml) Takyon No ROX Probe 5X HTP MasterMix dttp UF-NP5X-CG 5 RXN (5 x ml) UF-NPCT-B Takyon No ROX Probe X Core Kit BLUE dttp 5 RXN Takyon for SYBR Assays UF-RSMT-B Takyon ROX SYBR X MasterMix BLUE dttp UF-RSMT-B7 Option PREVENT Easily convert any dttp kit into a carryover kit with a single pipeting step using the dutp/ung additive [RT-UTPUNG-]. Option CONVERT With the Takyon One-Step Kit Converter convert any dttp X MasterMix into a One-Step qpcr Kit [UF-RTAD-D UF-RTAD-D7] UF-RSCT-B Takyon Rox SYBR X Core Kit BLUE dttp 5 RXN Low ROX UF-LSMT-B UF-LSMT-B7 Takyon Low ROX SYBR X MasterMix BLUE dttp No ROX Fluorescein UF-NSMT-B Takyon No Rox SYBR X MasterMix BLUE dttp UF-NSMT-B7 UF-NSCT-B Takyon No Rox SYBR X Core Kit BLUE dttp 5 RXN UF-FSMT-B Takyon SYBR X MasterMix BLUE dttp with fluorescein UF-FSMT-B7 #6

7 Universal qpcr kits for SYBR & Probe Assays Takyon TM One-Step The Takyon One-Step MasterMixes contain the new EuroscriptII RT which delivers highly robust performance on classical mrnas, small RNA and viral RNAs. The new additive improves significantly the amplification performance on difficult virus types as well as on FFPE samples. The 5X highly concentrated format allows more flexibility in template volume use especially in the context of small volume reaction. Perfectly suited for GENE EXPRESSION studies, Catalogue number Takyon TM One-Step Probe 5X Kits qrt-pcr kits ROX Low ROX No ROX for Probe Assays UF-RP5X-RT5 5 RXN (.6 ml) Takyon One-Step ROX Probe 5X MasterMix dttp UF-RP5X-RT 5 RXN (5 x ml) UF-LP5X-RT5 5 RXN (.6 ml) Takyon One-Step Low ROX Probe 5X MasterMix dttp UF-LP5X-RT 5 RXN (5 x ml) UF-NP5X-RT5 5 RXN (.6 ml) Takyon One-Step No ROX Probe 5X MasterMix dttp UF-NP5X-RT 5 RXN (5 x ml) Takyon TM One-Step SYBR & Probe X Kits Combine any dttp Takyon TM MasterMix with the One-Step kit converter UF-RTAD-D UF-RTAD-D7 Takyon TM One-Step Kit converter μl for 5 RXN MMix for 75 RXN MMix Related products and services - qpcr plates - qpcr controls - Custom primers & probes - Access dyes & quenchers - MGB probes - Assay dispensing services For research use only. notice to purchaser: limited license Licensed dsdna-binding Dye Research Kits - Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Dierctor of Licensing, Applied Biosystems, 85 Lincoln Centre Drive, Foster City, California 9, USA. UDG - Use of UDG employs U.S. Patents 5,5,996, 5,95,, 5,68,896 and their foreign counterparts licensed to Eurogentec S.A. from Invitrogen Corporation. Rox Passive Reference - Purchase of this product includes an immunity from suit to use only the amount purchased for the purchaser s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. For information about these rights or on obtaining additional rights, please contact outlicensing@lifetech.com or Out Licensing, Life Technologies, 579 Van Allen Way, Carlsbad, California 98. Trademarks information available onwww.eurogentec.com/legal-notices.html

8 Also Available: - HTP MasterMix convenient for assay set-up automation - Custom formulations on request Please visit us: Follow us on How to order? Europe isales@eurogentec.com Need support? info@eurogentec.com [European Toll Free Number] How to order? America service@anaspec.com service@anaspec.com [Toll Free Number] GRT-TAKYON PROBE-5-V6 - Eurogentec is part of Kaneka