enzyme technologies Catalogue 2014 R&D Services protein analysis and engineering For service updates visit

Transcription

1 enzyme technologies Catalogue 2014 R&D Services protein analysis and engineering For service updates visit

2 Content Company profile 3 Protein production 4 Protein analysis 6 Protein engineering and screening 8 Molecular modelling and bioinformatics 9 2

3 Company profile Enantis is the Czech R&D company founded in 2006 as the first biotechnology spin-off from Masaryk University in Brno. Enantis provides consulting and development services in the field of enzyme technologies and protein engineering for biomedical, environmental, agrochemical and military-defence applications. Employing the latest technologies, the company offers its own range of products based on enzymes haloalkane dehalogenases. Enantis cooperates tightly with the Loschmidt Laboratories at Masaryk University that are qualified guarantor of provided services and have core competency in the research of enzymes haloalkane dehalogenases. Customers are Enantis first priority. Main goal is to provide our customers with the highest quality products and services at competitive prices. With over twenty years work in the field of bimolecular sciences, we offer a wealth of experience to meet your milestones and deliver regulatory compliant data. Website: Online Product Support Product brochures, MSDS and the latest catalogues are available for download online. News Check out for the latest promotions or offers. See where you can meet Enantis representatives on conferences and fairs. 3

4 Protein production Enantis provides tailored solutions for efficient production of enzymes for biotechnological applications. Upon request, a biocatalyst of choice can be isolated, purified, lyophilized and immobilized to meet customers demands. Protein expression recombinant DNA technology SDS-electrophoresis and densitometry cultivation in shake flasks batch and fed-batch fermentation» cloning of gene into expression vectors» verification of gene sequence» transformation of constructs into host cells» optimization of protein expression conditions Protein separation and purification sonication homogenization centrifugation membrane filtration salt precipitation chromatography» cell disruption» protein separation and removal of insoluble aggregates» protein purification by chromatographic techniques» protein dialysis 4

5 Protein production Protein lyophilization and immobilization vacuum freeze-drying cross-linking by chemical cross-linkers covalent binding to functionalized glass surfaces preparation of cross-linked enzyme aggregates entrapment in polyvinyl alcohol particles entrapment in inorganic-organic hybrid polymers» preservation of protein by lyophilization» stabilization of protein by immobilization» determination of activity and leaching after immobilization» determination of long-term stability of immobilized enzyme» determination of thermal stability of immobilized enzyme 5

6 Protein analysis Enantis offers protein analysis services including determination of quantity, purity, physicochemical properties and biological activity of proteins. Protein stability can be determined under various conditions to obtain important data on structure-stability relationships. Analysis of protein physico-chemical properties SDS electrophoresis and densitometry gel filtration with light scattering native gel electrophoresis UV-visible spectroscopy» determination of protein quantity» determination of protein purity» determination of molecular weight» determination of oligomeric state Analysis of protein structure and stability far- and near-uv circular dichroism spectroscopy fluorescence spectroscopy differential scanning calorimetry» determination of protein secondary and tertiary structure» comparison of proteins from different expression systems» comparison of wild type and mutant proteins» analysis of protein stability under stress conditions» analysis of protein structures in various buffers and additives» analysis of protein-protein and protein-ligand interactions 6

7 Protein analysis Analysis of protein function isothermal titration microcalorimetry stopped-flow and rapid quench flow gas chromatography gas chromatography-mass spectrometry fluorescence spectroscopy UV-visible spectroscopy» analysis of protein-ligand interactions» analysis of protein-protein interactions» substrate specificity profiling» determination enzymatic activities» determination steady-state kinetics» determination pre-steady-state kinetics» determination of inhibition constants» determination enantio- and regioselectivity» determination temperature and ph profiles» determination protein half-lives 7

8 Protein engineering & screening Enantis uses rational design and directed evolution to create the optimized biocatalysts meeting demands of our costumers. Molecular biology techniques, robotic high-throughput screening and microfluidics can be employed for fast optimization of protein sequence. Protein engineering site-directed mutagenesis directed evolution focused directed evolution» construction of mutants with improved activities» construction of mutants with improved stabilities» construction of mutants with improved enantioselectivities High-throughput screening colony picking robotic system liquid handling robotic system fluorescence-activated cell sorting microfluidics» screening for novel biocatalysts» screening for improved activities» screening for enhanced stabilities 8

9 Molecular modelling & bioinformatics Enantis offers molecular modelling and bioinformatics analyses for life science research. A broad range of analyses can be carried out to address important biological questions in the areas of structure-function predictions, drug discovery and protein engineering. Molecular modelling homology modeling and de novo protein design molecular docking and virtual screening molecular dynamics simulations quantum mechanics calculations quantitative structure activity relationships» prediction of 3D structure of proteins and their complexes» prediction of transport pathways in biomolecules» estimation of free energies of biochemical reactions» identification of functional residues and design of mutations» prediction of novel inhibitors and substrates» construction of predictive statistical models Bioinformatic analysis genomic database searches phylogenetic analysis multivariate statistical analysis» identification of new family members» prediction of protein structure and function» prediction of posttranslational modifications» prediction of ancestors and evolutionary analyses 9

10 Enantis, s.r.o. INBIT, Kamenice 34, Brno, Czech Republic Phone: