Collecting Blood for ips Cell Reprogramming USA

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Collecting Blood for ips Cell Reprogramming USA"

Transcription

1 MyCell Products Application Protocol Collecting Blood for ips Cell Reprogramming USA Introduction: Blood Collection and Infectious Disease Testing All persons collecting and handling any blood samples should be appropriately trained regarding (A) the safe handling and administration of blood and blood components, (B) bloodborne pathogen safety and exposure controls (tasks and procedures to minimize human exposure to such pathogens), and (C) any and all other requirements under all applicable laws and regulations and guidance as to best safety practices. Note: For clarification on this protocol, and request the MyCell Products Webinar: Getting Started. A trained phlebotomist must collect blood samples using the Vacutainer tubes included in the MyCell Blood Donor Collection Kit. All blood samples will undergo infectious disease testing (IDT) after receipt by Cellular Dynamics International (CDI): Ship the fresh blood to CDI within 24 hours of collection. The blood must be determined free of HBV, HCV, and HIV. IDT Name Hepatitis B Surface Antigen Hepatitis C Antibody HIV Antigen and Antibody Relative Current Procedural Terminology (CPT) Code In the case of a positive IDT result, CDI will discard the donor s samples. Required Equipment and Consumables The following equipment and consumables are required for collecting blood. Item Equipment Tabletop Centrifuge with Proper Adaptors for Vacutainer Tubes and Capable of Maintaining 4 C 1 Vendor Multiple Vendors Collecting Blood for ips Cell Reprogramming USA 1

2 Item Consumables MyCell Blood Donor Collection Kit 2,3,4 Vendor Cellular Dynamics International (CDI) 1 CDI recommends using a USA E8 Fixed Speed Centrifuge (LW Scientific, Cat. No. E8C-U8AF-1503) or an equivalent centrifuge. 2 Do not discard any materials included in the kit as they may be used for sample identification and shipment. 3 Store the gel packs at 4 C until ready to use. Do not freeze the gel packs. 4 Store the Vacutainer tubes at room temperature and use before the expiration date printed on the tube. The kit includes Vacutainer Lithium Heparin (BD Biosciences, Cat. No ), CPT (BD Biosciences, Cat. No ), and SST (BD Biosciences, Cat. No ) tubes. Notes Workflow Blood samples are collected in the Vacutainer tubes: 1 Lithium Heparin tube for DNA extraction, 1 SST tube for IDT, and 4 CPT tubes for reprogramming. Fresh blood samples are shipped to CDI within 24 hours of collection. Methods: Collecting Blood Samples The MyCell Blood Donor Collection Kit includes the Vacutainer Lithium Heparin, SST, and CPT tubes for blood samples. The kit also includes the MyCell Donor Sample labels with CDI-assigned ID and project numbers for sample identification. The phlebotomist should collect 1 Lithium Heparin tube (4 ml/tube), 1 SST tube (4 ml/tube), and 4 CPT tubes (8 ml/tube) from each donor. Note: Store the Vacutainer tubes at room temperature until ready to perform blood collection. Use before the expiration date printed on the tube. 1. Label each Vacutainer tube with a CDI-provided MyCell Donor Sample label (Figure 1). Figure 1: Labeling Vacutainer Tubes Apply a CDI-provided MyCell Donor Sample label to each tube to ensure proper identification. 2 Cellular Dynamics International, Inc.

3 Notes 2. Collect the blood samples by venipuncture using standard blood drawing procedures. Immediately invert the Lithium Heparin tube 8-10 times to ensure complete mixing. Invert the CPT and SST tubes 5 times to ensure complete mixing. 3. Maintain the Lithium Heparin tube on ice during incubation and subsequent centrifugation of CPT and SST tubes. Incubate the CPT and SST tubes at room temperature for minutes. Note: See Appendix A for recommendations on properly handling and processing SST tubes. 4. Centrifuge the CPT and SST tubes at room temperature using the speed and duration, as specified in the table below, for the centrifuge s rotor type. Do not centrifuge the Lithium Heparin tube. Note: See the manufacturer s instructions for adaptors needed to centrifuge Vacutainer tubes. Note: Ensure the rotor is correctly balanced to achieve proper blood separation. Vacutainer Tube Speed (x g) Duration (min) Swinging Bucket Rotor Fixed Angle Rotor CPT Tube SST Tube Inspect the CPT and SST tubes to ensure proper blood separation was achieved (Figure 2). Note: If the blood did not separate into phases, ensure the rotor is balanced and centrifuge the tubes once more; however, be aware there will be a reduction in the number of PBMCs isolated. If the blood still does not separate, collect a fresh sample from the same donor. CPT Tube SST Tube Figure 2: Vacutainer CPT and SST Tubes after Blood Centrifugation The blood separates into phases upon centrifugation. 6. Maintain the Lithium Heparin, SST, and CPT tubes on ice until ready to package for shipment. Note: Samples will be maintained on gel packs during shipment. See the packaging and shipping instructions, below. Collecting Blood for ips Cell Reprogramming USA 3

4 Sample Shipment: Shipping Fresh Blood The MyCell Blood Donor Collection Kit includes the packaging materials. Ship fresh blood with 24 hours of collection by FedEx for overnight delivery to CDI. Please ensure arrival on or before the Friday of the same week. Do not ship samples on a Friday. 1. Equilibrate the 2 gel packs at 4 C overnight. Note: Maintain the gel packs at 4 C until ready to use. Do not freeze the gel packs. 2. Download and complete the CDI Shipping Manifest: Note: See Appendix B for an example of the form. 3. Package the samples (Figure 3): a. Place the insulated shipper in the outer shipping box. b. Add a layer of bubble wrap to the bottom of the insulated shipper. c. Wrap the Vacutainer tubes in absorbent paper and place in the small vial carton. d. Place the small vial carton containing the Vacutainer tubes in a sealable plastic bag and then in the insulated shipper. e. Add another layer of bubble wrap and place 2 cold gel packs on top of the bubble wrap before sealing the insulated shipper s lid. 4. Place a printout of the completed CDI Shipping Manifest in the outer shipping box and in the site study binder. 5. Before sample shipment, the FedEx tracking number and the CDI Shipping Manifest to This notification allows CDI to track and prepare for delivery of the shipment. 6. Ship by FedEx for overnight delivery to: Cellular Dynamics International c/o AllCells (CDI MyCell 2015 Project) 1301 Harbor Bay Parkway, Suite 200 Alameda, CA Notes A B C D E Figure 3: Shipping Vacutainer Tubes Containing Fresh Blood Package the tubes for shipment as follows: (A) Place the insulated shipper with a layer of bubble wrap in the outer shipping box. (B) Wrap the tubes in absorbent paper and place in the small vial carton. (C) Place the small vial carton in a sealable plastic bag and then in the insulated shipper. (D) Add another layer of bubble wrap followed by 2 cold gel packs. (E) Seal the insulated shipper s lid. (Not shown) Place a printout of the completed CDI Shipping Manifest in the outer shipping box before sealing. 4 Cellular Dynamics International, Inc.

5 Notes Appendices Appendix A. Proper Handling of Vacutainer SST Tubes See the next page for the November 2005 issue of Tech Talk (courtesy of Becton, Dickinson and Company), which details how to handle and process Vacutainer SST tubes. Collecting Blood for ips Cell Reprogramming USA 5

6 6 Cellular Dynamics International, Inc. Notes

7 Notes Appendix B. Example CDI Shipping Manifest Collecting Blood for ips Cell Reprogramming USA 7

8 Notes Customer s Responsibilities CDI does not guarantee that you will obtain equivalent results from using icell or MyCell products as described herein or that such use will not infringe any intellectual property right(s) of any third party(ies). You are solely responsible for obtaining any licenses you may require for your specific research use(s) of the icell or MyCell products not expressly conveyed under CDI s terms and conditions of sale or other transfer of the icell or MyCell products to you. Conditions of Use For life science research use only. Trademarks icell is a registered trademark, and Cellular Dynamics and the logo are trademarks of Cellular Dynamics International, Inc. All other brands, product names, company names, trademarks, and service marks are the properties of their respective owners. Copyright Notice 2016 Cellular Dynamics International, Inc. All rights reserved. Revision History Version 1.0: December 2016 AP-MPBCUSA Cellular Dynamics International, Inc.

STEP-BY-STEP INSTRUCTIONS FOR INVESTIGATIONAL USE. Rapid HCV Antibody Test FOR ORAQUICK RAPID HCV ANTIBODY TEST

STEP-BY-STEP INSTRUCTIONS FOR INVESTIGATIONAL USE. Rapid HCV Antibody Test FOR ORAQUICK RAPID HCV ANTIBODY TEST Before performing testing, all operators MUST read and become familiar with Universal Precautions for Prevention of Transmission of Human Immunodeficiency Virus, Hepatitis B Virus, and other Blood-borne

More information

Please note: Contact Coppe Laboratories at 262-574-0701 if archival plasma samples need to be tested.

Please note: Contact Coppe Laboratories at 262-574-0701 if archival plasma samples need to be tested. Collecting a Coppe Laboratories Sample The Coppe Laboratories Sample Kit contains: Test Request Form (TRF) Heparin tube Absorbent sheet Biohazard bag Foil pouch Label for blood tube Box Seal Instructions

More information

Classic Immunoprecipitation

Classic Immunoprecipitation 292PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Classic Immunoprecipitation Utilizes Protein A/G Agarose for Antibody Binding (Cat.

More information

Packaging Perishable Shipments

Packaging Perishable Shipments Packaging Perishable Shipments To help prevent spoilage and maintain product integrity, follow these instructions for shipping your perishable items via FedEx Express services. Preparing Perishable Items

More information

PEAC CENTRAL LABORATORY SAMPLING MANUAL

PEAC CENTRAL LABORATORY SAMPLING MANUAL PEAC CENTRAL LABORATORY SAMPLING MANUAL FINAL REVISED VERSION (9.0) PEAC Clinical Study Final 1.0 2 TABLE OF CONTENTS Page No. 1. PEAC CENTRAL LABORATORY - CONTACT NUMBERS... 3 2. IMPORTANT INFORMATION...

More information

Standard Operating Procedure (SOP) Work Package 8. Sample Collection and Storage

Standard Operating Procedure (SOP) Work Package 8. Sample Collection and Storage Standard Operating Procedure (SOP) Work Package 8 Sample Collection and Storage May 2008 SOP WP 8 - Sample collection Table of Contents 1 SAMPLE COLLECTION... 3 1.1 Sample collection -summary... 3 1.2

More information

GTEx Work Instruction for Whole Brain, Brain Stem, and Hair Collection Receipt and Shipping (Green Kit)

GTEx Work Instruction for Whole Brain, Brain Stem, and Hair Collection Receipt and Shipping (Green Kit) OP-0001-W2 VER. 03.08 Effective Date: mm/dd/yyyy Page 1 of 7 1.0 PURPOSE 1.1 This work instruction provides the specific steps necessary to pack and ship the Comprehensive Biospecimen Resource (CBR)-issued

More information

Packaging Perishable Shipments

Packaging Perishable Shipments Packaging Perishable Shipments To help prevent spoilage and maintain product integrity, follow these instructions for shipping your perishable items via FedEx Express services. Preparing Perishable Items

More information

VACUETTE RNAgard Blood Tubes BioMaxi Blood RNA Purification Kit. For the collection, preservation and purification of RNA from whole blood

VACUETTE RNAgard Blood Tubes BioMaxi Blood RNA Purification Kit. For the collection, preservation and purification of RNA from whole blood RNAgard Blood System VACUETTE RNAgard Blood Tubes BioMaxi Blood RNA Purification Kit For the collection, preservation and purification of RNA from whole blood Instructions For Use Protocol blood collection,

More information

Instructions for Drawing Blood Samples

Instructions for Drawing Blood Samples Instructions for Drawing Blood Samples For Blood Collection Supplies please contact us. *Whatever means of restraint is used it should cause minimal distress and should be quick and safe for both the animal

More information

Genetically Modified shipping quick reference. And Shipping labels

Genetically Modified shipping quick reference. And Shipping labels Genetically Modified shipping quick reference And Shipping labels Print this page and cut out labels to affix to box. (packing tape is recommended) Use wide clear tape to completely cover labels to protect

More information

CHAPTER 5 SHIPPING PROCEDURES

CHAPTER 5 SHIPPING PROCEDURES CHAPTER 5 SHIPPING PROCEDURES Chapter 5 SHIPPING PROCEDURES 5.1 SHIPPING CBUS FROM COLLECTION CENTERS TO CORD BLOOD BANKS Principle Collected umbilical cord blood has been found to maintain cell viability

More information

Non-Alberta Healthcare Testing Laboratory Manual

Non-Alberta Healthcare Testing Laboratory Manual Non-Alberta Healthcare Testing Laboratory Manual Developed and Supplied by: DynaLIFE Dx Suite 200, 10150 102 Street Edmonton AB T5J 5E2 Page i 1.0 Introduction for Laboratory Services Table of Contents

More information

(800) 786-7235 Cryo-Cell.com

(800) 786-7235 Cryo-Cell.com HEALTHCARE PROVIDER Instructions Keep these instructions in the kit [ When handling blood products, use personal protective measures, maintain aseptic techniques and adequate room for collection processes.

More information

Pointers on Shipping: Clinical Samples, Biological Substance Category B(UN 3373) and Environmental Test Samples

Pointers on Shipping: Clinical Samples, Biological Substance Category B(UN 3373) and Environmental Test Samples Pointers on Shipping: Clinical Samples, Biological Substance Category B(UN 3373) and Environmental Test Samples At FedEx, we understand the importance of ensuring the safe shipping of clinical samples

More information

Human Peripheral Blood Mononuclear Cell (PBMC) Manual

Human Peripheral Blood Mononuclear Cell (PBMC) Manual Human Peripheral Blood Mononuclear Cell (PBMC) Manual INSTRUCTION MANUAL ZBM0063.04 SHIPPING CONDITIONS Human Peripheral Blood Mononuclear Cells, cryopreserved Cryopreserved human peripheral blood mononuclear

More information

VialSafe. DHL VialSafe. UN3373 Packaging Solutions

VialSafe. DHL VialSafe. UN3373 Packaging Solutions VialSafe DHL VialSafe UN3373 Packaging Solutions The DHL VialSafe pack is a complete packaging system certified to meet the strict requirements of ICAO/IATA packing instructions PI650 for the safe transportation

More information

Agencourt RNAdvance Blood Kit for Free Circulating DNA and mirna/rna Isolation from 200-300μL of Plasma and Serum

Agencourt RNAdvance Blood Kit for Free Circulating DNA and mirna/rna Isolation from 200-300μL of Plasma and Serum SUPPLEMENTAL PROTOCOL WHITE PAPER Agencourt RNAdvance Blood Kit for Free Circulating DNA and mirna/rna Isolation from 200-300μL of Plasma and Serum Bee Na Lee, Ph.D., Beckman Coulter Life Sciences Process

More information

Shipping of Infectious Substances and Patient Specimens

Shipping of Infectious Substances and Patient Specimens Shipping of Infectious Substances and Patient Specimens If you ship infectious substances or patient specimens you should be aware of the International Air Transport Association (IATA) Dangerous Goods

More information

Parent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood

Parent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood Parent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood 1 2 3 Contents List of Umbilical Cord Blood Collection Kit Thermally insulated transportation box - do

More information

UC Davis Guide to Shipping Dry Ice REVISED 06/10/2015 JDC

UC Davis Guide to Shipping Dry Ice REVISED 06/10/2015 JDC UC Davis Guide to Shipping Dry Ice REVISED 06/10/2015 JDC 1 Table of Contents Introduction.3 Training Objectives...4 Training Requirements.5 Dry Ice Hazards. 6 Identifying/Classifying Dry Ice.7-12 Packaging

More information

CDC-Lipid Standardization Program. Laboratory Data Collection System (LDCS) Instruction Manual

CDC-Lipid Standardization Program. Laboratory Data Collection System (LDCS) Instruction Manual CDC-Lipid Standardization Program Laboratory Data Collection System (LDCS) Instruction Manual CDC Lipid Standardization Program Read Directions Carefully Before Sample Analysis CDC-LSP Laboratory Data

More information

Guidelines for Handling and Shipping of Blood Samples

Guidelines for Handling and Shipping of Blood Samples Biofocus Institute for Laboratory Medicine Dr. med. Doris Bachg Dr. med. Uwe Haselhorst Berghäuser Str. 295 45659 Recklinghausen Germany contact: Dr. Doris Bachg (bachg@biofocus.de) phone +49 2361 3000-144

More information

INTRODUCTION. Viral shedding is crucial for Gene Therapy Products Safety linked with shedding data

INTRODUCTION. Viral shedding is crucial for Gene Therapy Products Safety linked with shedding data INTRODUCTION Viral shedding is crucial for Gene Therapy Products Safety linked with shedding data For the patient For families and friends For the environment No treatment possible without good safety

More information

Guideline for Shipping Items 1 on Dry Ice that are Not Dangerous Goods

Guideline for Shipping Items 1 on Dry Ice that are Not Dangerous Goods Guideline for Shipping Items 1 on Dry Ice that are Not Dangerous Goods INTRODUCTION Dry ice is a hazardous material; therefore the U.S. Department of Transportation (DOT) and the International Air Transport

More information

ab185915 Protein Sumoylation Assay Ultra Kit

ab185915 Protein Sumoylation Assay Ultra Kit ab185915 Protein Sumoylation Assay Ultra Kit Instructions for Use For the measuring in vivo protein sumoylation in various samples This product is for research use only and is not intended for diagnostic

More information

Infectious Waste Disposal Procedure 25 PA Code 284 Ursinus College Collegeville, PA 19426

Infectious Waste Disposal Procedure 25 PA Code 284 Ursinus College Collegeville, PA 19426 The purpose of the Infectious Waste Disposal procedure is to establish procedures for the safe handling and proper disposal of infectious waste generated at in accordance with regulation 25 PA Code Chapter

More information

Family Cord Blood Stem Cells Bank

Family Cord Blood Stem Cells Bank Family Cord Blood Stem Cells Bank Hope for life......evolves from life itself WHAT ARE STEM CELLS AND WHY ARE THEY PRESERVED? Cord blood stem cells are in the greatest percentage haematopoetic stem cells

More information

BioResearch. RAFT 3D Cell Culture Kit Protocol

BioResearch. RAFT 3D Cell Culture Kit Protocol BioResearch RAFT 3D Cell Culture Kit Protocol RAFT 3D Cell Culture Kit This protocol describes in detail how to produce RAFT 3D Cell Cultures in 96-well plates, 24-well plates and 24-well inserts. It is

More information

WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots. Fluorescent detection via: Goat anti-mouse SureLight P3

WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots. Fluorescent detection via: Goat anti-mouse SureLight P3 WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots Fluorescent detection via: Goat anti-mouse SureLight P3 Cat. #: WK-P112 6440 Dobbin Road, Suite D Phone (443)

More information

HBV Quantitative Real Time PCR Kit

HBV Quantitative Real Time PCR Kit Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative Real Time PCR Kit Cat. No.: HD-0002-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems (Pls ignore

More information

RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method)

RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Immune Tolerance Network RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Author: Paul Wallace, Director, RPCI Laboratory of Flow Cytometry Approved by: Paul

More information

Culture and Differentiation of Axol TM Human Neural Progenitor Cells (hnpcs)

Culture and Differentiation of Axol TM Human Neural Progenitor Cells (hnpcs) Culture and Differentiation of Axol TM Human Neural Progenitor Cells (hnpcs) Catalog nos. ax0013-ax0016 ax0111-ax0115, ax0031a&b, ax0041-ax0045, ax0047 Instruction Manual Version 2.5 22/07/2014 Table of

More information

Blood Collection and Processing SOP

Blood Collection and Processing SOP Brisbane Breast Bank Blood Collection and Processing SOP Breast Pathology Laboratory University of Queensland Centre for Clinical Research Blood Collection We collect 30ml of blood from patients who have

More information

Maxwell 16 Blood DNA Purification System

Maxwell 16 Blood DNA Purification System Technical Manual Maxwell 16 Blood DNA Purification System Caution: Handle cartridges with care; seal edges may be sharp. 2800 Woods Hollow Rd. Madison, WI USA In Vitro Diagnostic Medical Device MDSS GmbH

More information

Protocol v002 Page 1 of 7 Agencourt RNAdvance Cell v2 Total RNA Isolation from Cultured Cells

Protocol v002 Page 1 of 7 Agencourt RNAdvance Cell v2 Total RNA Isolation from Cultured Cells Page 1 of 7 Agencourt RNAdvance Cell v2 Total RNA Isolation from Cultured Cells Please refer to http://www.agencourt.com/technical for updated protocols and refer to MSDS instructions http://www.beckmancoulter.com/customersupport/msds/msds.asp

More information

HAZARDOUS MATERIAL SAFETY PROCEDURES

HAZARDOUS MATERIAL SAFETY PROCEDURES HAZARDOUS MATERIAL SAFETY PROCEDURES Effective Date: January 1, 1992 Revised Date: March 1993 UT Memphis shall implement a program that protects its employees from hazardous chemical in accordance with

More information

Gravity Bag Cord Blood Collection Method Training Manual

Gravity Bag Cord Blood Collection Method Training Manual Gravity Bag Cord Blood Collection Method Training Manual CordBank Ltd. Training Manual Page 1 of 13 Table of Contents Chapter Page Introduction 3 Requirements for Collection of Cord Blood 3 Collector s

More information

VS Guidance 6702.1 Date 04/26/13

VS Guidance 6702.1 Date 04/26/13 The IDEXX Antibody (Ab) Serological Test for Diagnosing Bovine Tuberculosis (TB) in TB-Affected Cattle Herds 1. Purpose and Background The caudal fold tuberculin skin test (CFT), the gamma interferon test,

More information

Instructions for Abroad Clinical Rotation

Instructions for Abroad Clinical Rotation Revised 7/12 Instructions for Abroad Clinical Rotation While travelling abroad you may have limited access to medical care. These instructions help prepare you for a blood/ body fluid exposure. Timely

More information

Human CD4+T Cell Care Manual

Human CD4+T Cell Care Manual Human CD4+T Cell Care Manual INSTRUCTION MANUAL ZBM0067.02 SHIPPING CONDITIONS Human CD4+T Cells, cryopreserved Cryopreserved human CD4+T cells are shipped on dry ice and should be stored in liquid nitrogen

More information

Expansion of Axol Human Neural Progenitor Cells (NSCs) Xeno-Free System

Expansion of Axol Human Neural Progenitor Cells (NSCs) Xeno-Free System Expansion of Axol Human Neural Progenitor Cells (NSCs) Xeno-Free System Instruction Manual Version 2 XF Protocol - 2 Table of Contents Product Information 3 Schematic Overview and Density Guidelines 4

More information

Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07

Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3

More information

THE PREPARATION OF SINGLE DONOR CRYOPRECIPITATE

THE PREPARATION OF SINGLE DONOR CRYOPRECIPITATE FACTS AND FIGURES JUNE 2004 NO 2 THE PREPARATION OF SINGLE DONOR CRYOPRECIPITATE Revised Edition Shân Lloyd National Blood Transfusion Service Zimbabwe Published by the World Federation of Hemophilia (WFH);

More information

Policy for the Disposal of Biological Waste

Policy for the Disposal of Biological Waste Policy for the Disposal of Biological Waste I. Biological Waste II. Regulated Medical Waste Prepared by: Rutgers Environmental Health and Safety 24 Street 1603 Building 4127, Livingston Campus Piscataway,

More information

Blood sampling and transportation Standard Operating Procedure

Blood sampling and transportation Standard Operating Procedure Blood sampling and transportation Standard Operating Procedure 1. Rationale for test 2. Requirements 3. Equipment 4. Method for blood taking 5. Instructions for Safe Box and transportation of samples 6.

More information

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605

Reference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605 LACTIC ACID Fasting, arterial specimen preferred. Please note whether arterial or venous. 0.5 ml heparinized plasma. Green top or PST must be drawn, placed on ice, and spun within 15 minutes. Immediately

More information

RealLine HCV PCR Qualitative - Uni-Format

RealLine HCV PCR Qualitative - Uni-Format Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013

More information

RealStar HBV PCR Kit 1.0 11/2012

RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße

More information

HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs)

HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) OBJECTIVE: can be cryopreserved in a liquid nitrogen (LN 2 ) freezer for long-term storage. This Standard Operating Procedure (SOP)

More information

Copyright 2009, International Society for Biological and Environmental Repositories (ISBER)

Copyright 2009, International Society for Biological and Environmental Repositories (ISBER) Logistics and Sample Transport: Overview, Recommendations, Validation Protocol International Society for Biological and Environmental Repositories (ISBER) Introduction Logistics and sample transport can

More information

Sample Exposure Control Plan

Sample Exposure Control Plan Sample Exposure Control Plan This sample Exposure Control Plan is provided as a guide to help employers create their own plan. Policy (Insert Facility/Company Name) are committed to providing its employees

More information

CHAPTER 3 COLLECTION PROCEDURES

CHAPTER 3 COLLECTION PROCEDURES CHAPTER 3 COLLECTION PROCEDURES Chapter 3 COLLECTION PROCEDURES 3.1 COLLECTION KIT Principle The cord blood collection kit will be carefully assembled by the Cord Blood Bank (CBB) supervisor or his/her

More information

Packaging Wine. Special arrangements are required for wine shipments. Please contact your FedEx account executive for more information.

Packaging Wine. Special arrangements are required for wine shipments. Please contact your FedEx account executive for more information. Packaging Wine To help maintain product integrity and reduce the risk of breakage, follow these instructions for packaging and shipping via FedEx Express services. Special arrangements are required for

More information

Attachment D Infection Control Policy METHODS OF IMPLEMENTATION AND CONTROL

Attachment D Infection Control Policy METHODS OF IMPLEMENTATION AND CONTROL Attachment D Infection Control Policy METHODS OF IMPLEMENTATION AND CONTROL OSHA requires that the ECP include a schedule and method of implementation for the various requirements of the standard. The

More information

Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual

Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.03 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+ Progenitor Cells, cryopreserved Cryopreserved human umbilical

More information

Clinical Waste Management & Sharps Injury Prevention. Clinical Skills

Clinical Waste Management & Sharps Injury Prevention. Clinical Skills Clinical Skills Clinical Waste Management and Sharps Injury Prevention Clinical Skills and Simulation Team Cardiff and Vale Waste Management Team Aims & Outcomes Aims & Outcomes The aim of this module

More information

General Information and Guidelines. Submission of blood tubes

General Information and Guidelines. Submission of blood tubes General Information and Guidelines Submission of blood tubes Note: Do not submit blood samples in syringes (especially with needles attached). Syringes that are submitted to the lab with the needles attached

More information

GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS

GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS ST-PETERSBURG STATE UNIVERSITY THEODOSIUS DOBZHANSKY CENTER FOR GENOME BIOINFORMATICS GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS 2015 The object of

More information

Affinity Chromatography

Affinity Chromatography PR100-02 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Affinity Chromatography Teacher s Guidebook (Cat. # BE-417) think proteins! think

More information

Appendix 2: Example Validation Plan

Appendix 2: Example Validation Plan Appendix 2: Example Validation Plan 1. Purpose / Scope This document describes the validation of: Shipper [enter shipper name] for the use in the inter-hospital/laboratory transport of [Enter components

More information

ab185916 Hi-Fi cdna Synthesis Kit

ab185916 Hi-Fi cdna Synthesis Kit ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1

More information

Optimal management of RA patients who require Biologic Therapy. (ORBIT Study) SAMPLING MANUAL

Optimal management of RA patients who require Biologic Therapy. (ORBIT Study) SAMPLING MANUAL Optimal management of RA patients who require Biologic Therapy (ORBIT Study) SAMPLING MANUAL VERSION (1.0) 2 TABLE OF CONTENTS Page No. 1. CENTRAL LABORATORY - CONTACT NUMBERS... 3 2. TO RE-ORDER SUPPLIES...

More information

NimbleGen DNA Methylation Microarrays and Services

NimbleGen DNA Methylation Microarrays and Services NimbleGen DNA Methylation Microarrays and Services Sample Preparation Instructions Outline This protocol describes the process for preparing samples for NimbleGen DNA Methylation microarrays using the

More information

Cold Agglutination Titer detecting Cold Reacting Antibodies

Cold Agglutination Titer detecting Cold Reacting Antibodies Objectives: Cold Agglutination Titer detecting Cold Reacting Antibodies 1. Perform a serial dilution to determine the amount of cold reacting antibody present in a patient specimen with the results obtained

More information

UltraClean Forensic DNA Isolation Kit (Single Prep Format)

UltraClean Forensic DNA Isolation Kit (Single Prep Format) UltraClean Forensic DNA Isolation Kit (Single Prep Format) Catalog No. Quantity 14000-10 10 preps 14000-S 1 prep Instruction Manual Please recycle Version: 10302012 1 Table of Contents Introduction...

More information

Genomic DNA Extraction Kit INSTRUCTION MANUAL

Genomic DNA Extraction Kit INSTRUCTION MANUAL Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview

More information

Follicle Dermal Papilla Cell

Follicle Dermal Papilla Cell Follicle Dermal Papilla Cell Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product Description

More information

EXPOSURE CONTROL PLAN (sample) 1 Child Care Directors and Employers

EXPOSURE CONTROL PLAN (sample) 1 Child Care Directors and Employers EXPOSURE CONTROL PLAN (sample) 1 Child Care Directors and Employers The Model Exposure Control Plan is intended to serve as an employer guide to the OSHA Bloodborne Pathogens standard. A central component

More information

Modulating Glucose Uptake in Skeletal Myotubes:

Modulating Glucose Uptake in Skeletal Myotubes: icell Skeletal Myoblasts Application Protocol Introduction Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis The skeletal muscle is one of the

More information

ISU College of Pharmacy Exposure Control Plan

ISU College of Pharmacy Exposure Control Plan Exposure Control Plan POLICY The College of Pharmacy at Idaho State University is committed to providing a safe and healthful work environment for our staff and students. In pursuit of this goal, the following

More information

KGI MEDICAL WASTE MANAGEMENT PLAN

KGI MEDICAL WASTE MANAGEMENT PLAN 1revised 2012 I. Facility Information KGI MEDICAL WASTE MANAGEMENT PLAN a. Contact Person: Barbara Erwin Director Research Operations/Chemical and Biological Laboratory Safety/Chair. 909-607-0160 i. Facility:

More information

Product Size Catalog Number. 500,000 proliferating cells

Product Size Catalog Number. 500,000 proliferating cells Pericytes Instruction Manual Product Size Catalog Number Human Pericytes from Placenta (hpc-pl) 500,000 cryopreserved cells 500,000 proliferating cells C-12980 C-12981 Product Description Pericytes are

More information

Content Sheet 5-1: Overview of Sample Management

Content Sheet 5-1: Overview of Sample Management Content Sheet 5-1: Overview of Management Role in quality management system management is a part of process control, one of the essentials of a quality management system. The quality of the work a laboratory

More information

One Shot TOP10 Competent Cells

One Shot TOP10 Competent Cells USER GUIDE One Shot TOP10 Competent Cells Catalog Numbers C4040-10, C4040-03, C4040-06, C4040-50, and C4040-52 Document Part Number 280126 Publication Number MAN0000633 Revision A.0 For Research Use Only.

More information

Quick Start Contents

Quick Start Contents Quick Start Guide 1 Quick Start Contents Contact Information.. 3 Create an Account 3 Order Saliva Collection Supplies...3 Collect a Specimen..4 Order a Test..5 Package and Ship Specimens...8 Schedule FedEx

More information

Human Adult Mesothelial Cell Manual

Human Adult Mesothelial Cell Manual Human Adult Mesothelial Cell Manual INSTRUCTION MANUAL ZBM0025.01 SHIPPING CONDITIONS Human Adult Mesothelial Cells Orders are delivered via Federal Express courier. All US and Canada orders are shipped

More information

Room Temp: 8 Hr. Room Temp: 8Hr. Room Temp: ASAP. Refrigerated (2-8C): 3D. Room Temp: ASAP. Refrigerated (2-8C): 3D

Room Temp: 8 Hr. Room Temp: 8Hr. Room Temp: ASAP. Refrigerated (2-8C): 3D. Room Temp: ASAP. Refrigerated (2-8C): 3D Diabetes Chem Panel (Glu, Trig, Chol, HDL, LDL, Chol:HDL Ratio, AST, ALT, BUN, Creat, GFR, Na, K, Cl, CO2, Ca) ANMC Lab Test Requirements Updated 4/7/15 Key: Room Temp (20-25C), Refrigerated (2-8C), (-15

More information

Packaging Guidelines for Battery Shipments

Packaging Guidelines for Battery Shipments Packaging Guidelines for Battery Shipments Follow these instructions to help ensure safe transportation of your battery shipments and your shipments with items containing batteries within the FedEx Express

More information

Cell Culture Protocol for Biogelx Peptide Hydrogel 2D and 3D Cell Culture PRO/BGX/001

Cell Culture Protocol for Biogelx Peptide Hydrogel 2D and 3D Cell Culture PRO/BGX/001 Protocol (PRO) Cell Culture Protocol for Hydrogel 2D and 3D Cell Culture PRO/BGX/001 Materials Provided Gel solution (stiffness of Gel will vary as per type of product requested), packaged in a glass vial.

More information

Anti-Treponema Syphilis IgM Cat # 1464Z

Anti-Treponema Syphilis IgM Cat # 1464Z DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

UCLA Henry Samueli School of Engineering and Applied Science STANDARD OPERATING PROCEDURE MEDICAL WASTE (BIOHAZARD WASTE) MANAGEMENT

UCLA Henry Samueli School of Engineering and Applied Science STANDARD OPERATING PROCEDURE MEDICAL WASTE (BIOHAZARD WASTE) MANAGEMENT UCLA Henry Samueli School of Engineering and Applied Science STANDARD OPERATING PROCEDURE MEDICAL WASTE (BIOHAZARD WASTE) MANAGEMENT PURPOSE The purpose of this document is to specify the procedures used

More information

Anti-Treponema Syphilis IgM. Cat # 1464Z

Anti-Treponema Syphilis IgM. Cat # 1464Z DIAGNOSTIC AUTOMATION, INC. 23961 Craftsman Road, Suite D/E/F, Calabasas, CA 91302 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com See external

More information

QuantiFERON TB Gold In Tube Method (QGIT Test) Manufacturer: Cellestis Inc. (USA) a QIAGEN Company

QuantiFERON TB Gold In Tube Method (QGIT Test) Manufacturer: Cellestis Inc. (USA) a QIAGEN Company QuantiFERON TB Gold In Tube Method (QGIT Test) Manufacturer: Cellestis Inc. (USA) a QIAGEN Company P1078 TB APPRISE TB Ante vs. Postpartum Prevention with INH in HIV Seropositive mothers and their Exposed

More information

Shipment of Perishable Products and Dry Ice Usage

Shipment of Perishable Products and Dry Ice Usage CPA Info # 81 December 2003 Shipment of Perishable Products and Dry Ice Usage Russ J. Bragg Extension Specialist Center for Profitable Agriculture Internet and mail order marketing strategies present individuals

More information

FLEX LED TAPE SERIES. ACCLAIM LIGHTING FLEX LED TAPE SERIES install guide 1.1

FLEX LED TAPE SERIES. ACCLAIM LIGHTING FLEX LED TAPE SERIES install guide 1.1 FLEX LED TAPE SERIES install guide 1.1 ACCLAIM LIGHTING FLEX LED TAPE SERIES install guide 1.1 2013 ACCLAIM LIGHTING all rights reserved. Information, specifications, diagrams, images, and instructions

More information

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.

IgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. IgM ELISA For the quantitative determination of IgM in human serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calibrator

More information

Equipment Qualification

Equipment Qualification Equipment Qualification Deborah L. Griffin, BA Laboratory Supervisor, HSCLab Hillman Cancer Center University of Pittsburgh Cancer Institute University of Pittsburgh Medical Center Equipment Qualification

More information

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr)

RNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr) RNA Extraction and Quantification, Reverse Transcription, and Real-time Preparation of Samples Cells: o Remove media and wash cells 2X with cold PBS. (2 ml for 6 well plate or 3 ml for 6cm plate) Keep

More information

Instruction Guide for Cleaning, Testing, and Packaging Kendall SCD 700 Sequential Compression System Controller and Tubing Set

Instruction Guide for Cleaning, Testing, and Packaging Kendall SCD 700 Sequential Compression System Controller and Tubing Set Instruction Guide for Cleaning, Testing, and Packaging Kendall SCD 700 Sequential Compression System Controller and Tubing Set TABLE OF CONTENTS Section 1 Instructions for UNUSED Units 2 Confirm Kit Materials

More information

www.maubookstore.com

www.maubookstore.com Millennia Atlantic University Students: YOU MAY RECEIVE YOUR REQUIRED TEXTBOOKS AND COURSE MATERIALS BY VISITING THE SCHOOL S OFFICIAL AT: www.maubookstore.com WHEN ORDERING YOU MAY REFER TO THE ATTTACHED

More information

Application of Axol ipsc-derived Cardiomyocytes on the xcelligence RTCA Cardio (ACEA Biosciences) With additional drug application protocol

Application of Axol ipsc-derived Cardiomyocytes on the xcelligence RTCA Cardio (ACEA Biosciences) With additional drug application protocol Application of Axol ipsc-derived Cardiomyocytes on the xcelligence RTCA Cardio (ACEA Biosciences) With additional drug application protocol Experts Protocol Series Application Protocol Kindly provided

More information

Health Care Workers in the Community

Health Care Workers in the Community Waste Management for Health Care Workers in the Community Adapted from Waste Management for Health Care Workers in the Community with the permission of Capital Health, Regional Public Health. Distributed

More information

No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer

No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer APPLICATION NOTE Attune NxT Flow Cytometer No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer Introduction Standard methods for isolating and detecting leukocytes

More information

New York State Department of Health (NYSDOH) Bureau of Immunization Vaccine Storage and Handling Plan

New York State Department of Health (NYSDOH) Bureau of Immunization Vaccine Storage and Handling Plan Vaccines need to be properly stored and administered to ensure maximum efficacy and safety. Check yes or no for each item. Implement changes for items marked no. Personnel: 1. We have designated a primary

More information

How to safely collect blood samples from persons suspected to be infected with highly infectious blood-borne pathogens (e.g.

How to safely collect blood samples from persons suspected to be infected with highly infectious blood-borne pathogens (e.g. How to safely collect blood samples from persons suspected to be infected with highly infectious blood-borne pathogens (e.g. Ebola) Step 1: Before entering patient room, assemble all equipment (1 st part)

More information

Carnegie Mellon University s Policy and Procedures for Recombinant and Synthetic Nucleic Acid Materials Spills

Carnegie Mellon University s Policy and Procedures for Recombinant and Synthetic Nucleic Acid Materials Spills Carnegie Mellon University s Policy and Procedures for Recombinant and Synthetic Nucleic Acid Materials Spills Background In accordance with Section IV-B-2-b-(6) of the NIH Guidelines for Research Involving

More information

Read Me First: Unpacking Express5800/320Fc System Cabinets

Read Me First: Unpacking Express5800/320Fc System Cabinets Read Me First: Unpacking Express5800/320Fc System Cabinets NEC Corporation of America 456-01721-000 Notice The information contained in this document is subject to change without notice. UNLESS EXPRESSLY

More information

Proto col. GoClone Repor ter Construc ts: Sample Protocol for Adherent Cells. Tech support: 877-994-8240. Luciferase Assay System

Proto col. GoClone Repor ter Construc ts: Sample Protocol for Adherent Cells. Tech support: 877-994-8240. Luciferase Assay System Luciferase Assay System Proto col GoClone Repor ter Construc ts: Sample Protocol for Adherent Cells LightSwitch Luciferase Assay System GoClone Reporter Assay Workflow Step 1: Seed cells in plate format.

More information

QIAGEN Supplementary Protocol

QIAGEN Supplementary Protocol QIAGEN Supplementary Protocol Isolation of Peripheral Blood Mononuclear Cells (PBMC) and Purification of Total RNA from PBMC Using the RNeasy Micro or Mini Kit This protocol describes how to isolate PBMC

More information