Collecting Blood for ips Cell Reprogramming USA
|
|
- Andrea Strickland
- 7 years ago
- Views:
Transcription
1 MyCell Products Application Protocol Collecting Blood for ips Cell Reprogramming USA Introduction: Blood Collection and Infectious Disease Testing All persons collecting and handling any blood samples should be appropriately trained regarding (A) the safe handling and administration of blood and blood components, (B) bloodborne pathogen safety and exposure controls (tasks and procedures to minimize human exposure to such pathogens), and (C) any and all other requirements under all applicable laws and regulations and guidance as to best safety practices. Note: For clarification on this protocol, and request the MyCell Products Webinar: Getting Started. A trained phlebotomist must collect blood samples using the Vacutainer tubes included in the MyCell Blood Donor Collection Kit. All blood samples will undergo infectious disease testing (IDT) after receipt by Cellular Dynamics International (CDI): Ship the fresh blood to CDI within 24 hours of collection. The blood must be determined free of HBV, HCV, and HIV. IDT Name Hepatitis B Surface Antigen Hepatitis C Antibody HIV Antigen and Antibody Relative Current Procedural Terminology (CPT) Code In the case of a positive IDT result, CDI will discard the donor s samples. Required Equipment and Consumables The following equipment and consumables are required for collecting blood. Item Equipment Tabletop Centrifuge with Proper Adaptors for Vacutainer Tubes and Capable of Maintaining 4 C 1 Vendor Multiple Vendors Collecting Blood for ips Cell Reprogramming USA 1
2 Item Consumables MyCell Blood Donor Collection Kit 2,3,4 Vendor Cellular Dynamics International (CDI) 1 CDI recommends using a USA E8 Fixed Speed Centrifuge (LW Scientific, Cat. No. E8C-U8AF-1503) or an equivalent centrifuge. 2 Do not discard any materials included in the kit as they may be used for sample identification and shipment. 3 Store the gel packs at 4 C until ready to use. Do not freeze the gel packs. 4 Store the Vacutainer tubes at room temperature and use before the expiration date printed on the tube. The kit includes Vacutainer Lithium Heparin (BD Biosciences, Cat. No ), CPT (BD Biosciences, Cat. No ), and SST (BD Biosciences, Cat. No ) tubes. Notes Workflow Blood samples are collected in the Vacutainer tubes: 1 Lithium Heparin tube for DNA extraction, 1 SST tube for IDT, and 4 CPT tubes for reprogramming. Fresh blood samples are shipped to CDI within 24 hours of collection. Methods: Collecting Blood Samples The MyCell Blood Donor Collection Kit includes the Vacutainer Lithium Heparin, SST, and CPT tubes for blood samples. The kit also includes the MyCell Donor Sample labels with CDI-assigned ID and project numbers for sample identification. The phlebotomist should collect 1 Lithium Heparin tube (4 ml/tube), 1 SST tube (4 ml/tube), and 4 CPT tubes (8 ml/tube) from each donor. Note: Store the Vacutainer tubes at room temperature until ready to perform blood collection. Use before the expiration date printed on the tube. 1. Label each Vacutainer tube with a CDI-provided MyCell Donor Sample label (Figure 1). Figure 1: Labeling Vacutainer Tubes Apply a CDI-provided MyCell Donor Sample label to each tube to ensure proper identification. 2 Cellular Dynamics International, Inc.
3 Notes 2. Collect the blood samples by venipuncture using standard blood drawing procedures. Immediately invert the Lithium Heparin tube 8-10 times to ensure complete mixing. Invert the CPT and SST tubes 5 times to ensure complete mixing. 3. Maintain the Lithium Heparin tube on ice during incubation and subsequent centrifugation of CPT and SST tubes. Incubate the CPT and SST tubes at room temperature for minutes. Note: See Appendix A for recommendations on properly handling and processing SST tubes. 4. Centrifuge the CPT and SST tubes at room temperature using the speed and duration, as specified in the table below, for the centrifuge s rotor type. Do not centrifuge the Lithium Heparin tube. Note: See the manufacturer s instructions for adaptors needed to centrifuge Vacutainer tubes. Note: Ensure the rotor is correctly balanced to achieve proper blood separation. Vacutainer Tube Speed (x g) Duration (min) Swinging Bucket Rotor Fixed Angle Rotor CPT Tube SST Tube Inspect the CPT and SST tubes to ensure proper blood separation was achieved (Figure 2). Note: If the blood did not separate into phases, ensure the rotor is balanced and centrifuge the tubes once more; however, be aware there will be a reduction in the number of PBMCs isolated. If the blood still does not separate, collect a fresh sample from the same donor. CPT Tube SST Tube Figure 2: Vacutainer CPT and SST Tubes after Blood Centrifugation The blood separates into phases upon centrifugation. 6. Maintain the Lithium Heparin, SST, and CPT tubes on ice until ready to package for shipment. Note: Samples will be maintained on gel packs during shipment. See the packaging and shipping instructions, below. Collecting Blood for ips Cell Reprogramming USA 3
4 Sample Shipment: Shipping Fresh Blood The MyCell Blood Donor Collection Kit includes the packaging materials. Ship fresh blood with 24 hours of collection by FedEx for overnight delivery to CDI. Please ensure arrival on or before the Friday of the same week. Do not ship samples on a Friday. 1. Equilibrate the 2 gel packs at 4 C overnight. Note: Maintain the gel packs at 4 C until ready to use. Do not freeze the gel packs. 2. Download and complete the CDI Shipping Manifest: Note: See Appendix B for an example of the form. 3. Package the samples (Figure 3): a. Place the insulated shipper in the outer shipping box. b. Add a layer of bubble wrap to the bottom of the insulated shipper. c. Wrap the Vacutainer tubes in absorbent paper and place in the small vial carton. d. Place the small vial carton containing the Vacutainer tubes in a sealable plastic bag and then in the insulated shipper. e. Add another layer of bubble wrap and place 2 cold gel packs on top of the bubble wrap before sealing the insulated shipper s lid. 4. Place a printout of the completed CDI Shipping Manifest in the outer shipping box and in the site study binder. 5. Before sample shipment, the FedEx tracking number and the CDI Shipping Manifest to mycell2015@allcells.com. This notification allows CDI to track and prepare for delivery of the shipment. 6. Ship by FedEx for overnight delivery to: Cellular Dynamics International c/o AllCells (CDI MyCell 2015 Project) 1301 Harbor Bay Parkway, Suite 200 Alameda, CA Notes A B C D E Figure 3: Shipping Vacutainer Tubes Containing Fresh Blood Package the tubes for shipment as follows: (A) Place the insulated shipper with a layer of bubble wrap in the outer shipping box. (B) Wrap the tubes in absorbent paper and place in the small vial carton. (C) Place the small vial carton in a sealable plastic bag and then in the insulated shipper. (D) Add another layer of bubble wrap followed by 2 cold gel packs. (E) Seal the insulated shipper s lid. (Not shown) Place a printout of the completed CDI Shipping Manifest in the outer shipping box before sealing. 4 Cellular Dynamics International, Inc.
5 Notes Appendices Appendix A. Proper Handling of Vacutainer SST Tubes See the next page for the November 2005 issue of Tech Talk (courtesy of Becton, Dickinson and Company), which details how to handle and process Vacutainer SST tubes. Collecting Blood for ips Cell Reprogramming USA 5
6 6 Cellular Dynamics International, Inc. Notes
7 Notes Appendix B. Example CDI Shipping Manifest Collecting Blood for ips Cell Reprogramming USA 7
8 Notes Customer s Responsibilities CDI does not guarantee that you will obtain equivalent results from using icell or MyCell products as described herein or that such use will not infringe any intellectual property right(s) of any third party(ies). You are solely responsible for obtaining any licenses you may require for your specific research use(s) of the icell or MyCell products not expressly conveyed under CDI s terms and conditions of sale or other transfer of the icell or MyCell products to you. Conditions of Use For life science research use only. Trademarks icell is a registered trademark, and Cellular Dynamics and the logo are trademarks of Cellular Dynamics International, Inc. All other brands, product names, company names, trademarks, and service marks are the properties of their respective owners. Copyright Notice 2016 Cellular Dynamics International, Inc. All rights reserved. Revision History Version 1.0: December 2016 AP-MPBCUSA Cellular Dynamics International, Inc.
STEP-BY-STEP INSTRUCTIONS FOR INVESTIGATIONAL USE. Rapid HCV Antibody Test FOR ORAQUICK RAPID HCV ANTIBODY TEST
Before performing testing, all operators MUST read and become familiar with Universal Precautions for Prevention of Transmission of Human Immunodeficiency Virus, Hepatitis B Virus, and other Blood-borne
More informationPlease note: Contact Coppe Laboratories at 262-574-0701 if archival plasma samples need to be tested.
Collecting a Coppe Laboratories Sample The Coppe Laboratories Sample Kit contains: Test Request Form (TRF) Heparin tube Absorbent sheet Biohazard bag Foil pouch Label for blood tube Box Seal Instructions
More informationPEAC CENTRAL LABORATORY SAMPLING MANUAL
PEAC CENTRAL LABORATORY SAMPLING MANUAL FINAL REVISED VERSION (9.0) PEAC Clinical Study Final 1.0 2 TABLE OF CONTENTS Page No. 1. PEAC CENTRAL LABORATORY - CONTACT NUMBERS... 3 2. IMPORTANT INFORMATION...
More informationClassic Immunoprecipitation
292PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Classic Immunoprecipitation Utilizes Protein A/G Agarose for Antibody Binding (Cat.
More informationPackaging Perishable Shipments
Packaging Perishable Shipments To help prevent spoilage and maintain product integrity, follow these instructions for shipping your perishable items via FedEx Express services. Preparing Perishable Items
More informationStandard Operating Procedure (SOP) Work Package 8. Sample Collection and Storage
Standard Operating Procedure (SOP) Work Package 8 Sample Collection and Storage May 2008 SOP WP 8 - Sample collection Table of Contents 1 SAMPLE COLLECTION... 3 1.1 Sample collection -summary... 3 1.2
More informationGTEx Work Instruction for Whole Brain, Brain Stem, and Hair Collection Receipt and Shipping (Green Kit)
OP-0001-W2 VER. 03.08 Effective Date: mm/dd/yyyy Page 1 of 7 1.0 PURPOSE 1.1 This work instruction provides the specific steps necessary to pack and ship the Comprehensive Biospecimen Resource (CBR)-issued
More informationVACUETTE RNAgard Blood Tubes BioMaxi Blood RNA Purification Kit. For the collection, preservation and purification of RNA from whole blood
RNAgard Blood System VACUETTE RNAgard Blood Tubes BioMaxi Blood RNA Purification Kit For the collection, preservation and purification of RNA from whole blood Instructions For Use Protocol blood collection,
More informationPackaging Perishable Shipments
Packaging Perishable Shipments To help prevent spoilage and maintain product integrity, follow these instructions for shipping your perishable items via FedEx Express services. Preparing Perishable Items
More informationCHAPTER 5 SHIPPING PROCEDURES
CHAPTER 5 SHIPPING PROCEDURES Chapter 5 SHIPPING PROCEDURES 5.1 SHIPPING CBUS FROM COLLECTION CENTERS TO CORD BLOOD BANKS Principle Collected umbilical cord blood has been found to maintain cell viability
More informationInstructions for Drawing Blood Samples
Instructions for Drawing Blood Samples For Blood Collection Supplies please contact us. *Whatever means of restraint is used it should cause minimal distress and should be quick and safe for both the animal
More information(800) 786-7235 Cryo-Cell.com
HEALTHCARE PROVIDER Instructions Keep these instructions in the kit [ When handling blood products, use personal protective measures, maintain aseptic techniques and adequate room for collection processes.
More informationPointers on Shipping: Clinical Samples, Biological Substance Category B(UN 3373) and Environmental Test Samples
Pointers on Shipping: Clinical Samples, Biological Substance Category B(UN 3373) and Environmental Test Samples At FedEx, we understand the importance of ensuring the safe shipping of clinical samples
More informationHuman Peripheral Blood Mononuclear Cell (PBMC) Manual
Human Peripheral Blood Mononuclear Cell (PBMC) Manual INSTRUCTION MANUAL ZBM0063.04 SHIPPING CONDITIONS Human Peripheral Blood Mononuclear Cells, cryopreserved Cryopreserved human peripheral blood mononuclear
More informationVialSafe. DHL VialSafe. UN3373 Packaging Solutions
VialSafe DHL VialSafe UN3373 Packaging Solutions The DHL VialSafe pack is a complete packaging system certified to meet the strict requirements of ICAO/IATA packing instructions PI650 for the safe transportation
More informationShipping of Infectious Substances and Patient Specimens
Shipping of Infectious Substances and Patient Specimens If you ship infectious substances or patient specimens you should be aware of the International Air Transport Association (IATA) Dangerous Goods
More informationCDC-Lipid Standardization Program. Laboratory Data Collection System (LDCS) Instruction Manual
CDC-Lipid Standardization Program Laboratory Data Collection System (LDCS) Instruction Manual CDC Lipid Standardization Program Read Directions Carefully Before Sample Analysis CDC-LSP Laboratory Data
More informationINTRODUCTION. Viral shedding is crucial for Gene Therapy Products Safety linked with shedding data
INTRODUCTION Viral shedding is crucial for Gene Therapy Products Safety linked with shedding data For the patient For families and friends For the environment No treatment possible without good safety
More informationParent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood
Parent & Healthcare Professional Instructions for the collection of Maternal & Umbilical Cord Blood 1 2 3 Contents List of Umbilical Cord Blood Collection Kit Thermally insulated transportation box - do
More informationUC Davis Guide to Shipping Dry Ice REVISED 06/10/2015 JDC
UC Davis Guide to Shipping Dry Ice REVISED 06/10/2015 JDC 1 Table of Contents Introduction.3 Training Objectives...4 Training Requirements.5 Dry Ice Hazards. 6 Identifying/Classifying Dry Ice.7-12 Packaging
More informationGuidelines for Handling and Shipping of Blood Samples
Biofocus Institute for Laboratory Medicine Dr. med. Doris Bachg Dr. med. Uwe Haselhorst Berghäuser Str. 295 45659 Recklinghausen Germany contact: Dr. Doris Bachg (bachg@biofocus.de) phone +49 2361 3000-144
More informationInstructions for Abroad Clinical Rotation
Revised 7/12 Instructions for Abroad Clinical Rotation While travelling abroad you may have limited access to medical care. These instructions help prepare you for a blood/ body fluid exposure. Timely
More informationAgencourt RNAdvance Blood Kit for Free Circulating DNA and mirna/rna Isolation from 200-300μL of Plasma and Serum
SUPPLEMENTAL PROTOCOL WHITE PAPER Agencourt RNAdvance Blood Kit for Free Circulating DNA and mirna/rna Isolation from 200-300μL of Plasma and Serum Bee Na Lee, Ph.D., Beckman Coulter Life Sciences Process
More informationab185915 Protein Sumoylation Assay Ultra Kit
ab185915 Protein Sumoylation Assay Ultra Kit Instructions for Use For the measuring in vivo protein sumoylation in various samples This product is for research use only and is not intended for diagnostic
More informationFamily Cord Blood Stem Cells Bank
Family Cord Blood Stem Cells Bank Hope for life......evolves from life itself WHAT ARE STEM CELLS AND WHY ARE THEY PRESERVED? Cord blood stem cells are in the greatest percentage haematopoetic stem cells
More informationHBV Quantitative Real Time PCR Kit
Revision No.: ZJ0002 Issue Date: Aug 7 th, 2008 HBV Quantitative Real Time PCR Kit Cat. No.: HD-0002-01 For Use with LightCycler 1.0/LightCycler2.0/LightCycler480 (Roche) Real Time PCR Systems (Pls ignore
More informationWESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots. Fluorescent detection via: Goat anti-mouse SureLight P3
WESTERN BLOT DETECTION KIT Buffers and detection reagents for up to ten 10 x 10 cm 2 blots Fluorescent detection via: Goat anti-mouse SureLight P3 Cat. #: WK-P112 6440 Dobbin Road, Suite D Phone (443)
More informationBioResearch. RAFT 3D Cell Culture Kit Protocol
BioResearch RAFT 3D Cell Culture Kit Protocol RAFT 3D Cell Culture Kit This protocol describes in detail how to produce RAFT 3D Cell Cultures in 96-well plates, 24-well plates and 24-well inserts. It is
More informationRPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method)
Immune Tolerance Network RPCI 004 v.002 Staining Procedure For all Directly Conjugated Reagents (Whole Blood Method) Author: Paul Wallace, Director, RPCI Laboratory of Flow Cytometry Approved by: Paul
More informationBlood Collection and Processing SOP
Brisbane Breast Bank Blood Collection and Processing SOP Breast Pathology Laboratory University of Queensland Centre for Clinical Research Blood Collection We collect 30ml of blood from patients who have
More informationMaxwell 16 Blood DNA Purification System
Technical Manual Maxwell 16 Blood DNA Purification System Caution: Handle cartridges with care; seal edges may be sharp. 2800 Woods Hollow Rd. Madison, WI USA In Vitro Diagnostic Medical Device MDSS GmbH
More informationVS Guidance 6702.1 Date 04/26/13
The IDEXX Antibody (Ab) Serological Test for Diagnosing Bovine Tuberculosis (TB) in TB-Affected Cattle Herds 1. Purpose and Background The caudal fold tuberculin skin test (CFT), the gamma interferon test,
More informationGravity Bag Cord Blood Collection Method Training Manual
Gravity Bag Cord Blood Collection Method Training Manual CordBank Ltd. Training Manual Page 1 of 13 Table of Contents Chapter Page Introduction 3 Requirements for Collection of Cord Blood 3 Collector s
More informationTHE PREPARATION OF SINGLE DONOR CRYOPRECIPITATE
FACTS AND FIGURES JUNE 2004 NO 2 THE PREPARATION OF SINGLE DONOR CRYOPRECIPITATE Revised Edition Shân Lloyd National Blood Transfusion Service Zimbabwe Published by the World Federation of Hemophilia (WFH);
More informationCreatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07
Creatine Kinase (CK) Enzymatic Assay Kit Manual Catalog #: 3460-07 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3
More informationPolicy for the Disposal of Biological Waste
Policy for the Disposal of Biological Waste I. Biological Waste II. Regulated Medical Waste Prepared by: Rutgers Environmental Health and Safety 24 Street 1603 Building 4127, Livingston Campus Piscataway,
More informationRealLine HCV PCR Qualitative - Uni-Format
Instructions for use PCR KIT FOR EXTRACTION OF RNA AND REAL TIME PCR DETECTION KIT FOR HEPATITIS C VIRUS RNA Research Use Only Qualitative Uni Format VBD0798 48 tests valid from: December 2013 Rev11122013
More informationBlood sampling and transportation Standard Operating Procedure
Blood sampling and transportation Standard Operating Procedure 1. Rationale for test 2. Requirements 3. Equipment 4. Method for blood taking 5. Instructions for Safe Box and transportation of samples 6.
More informationReference Range: 0.5-1.6 mmol/l (arterial) 0.7-2.1 mmol/l (venous) CPT Code: 83605
LACTIC ACID Fasting, arterial specimen preferred. Please note whether arterial or venous. 0.5 ml heparinized plasma. Green top or PST must be drawn, placed on ice, and spun within 15 minutes. Immediately
More informationRealStar HBV PCR Kit 1.0 11/2012
RealStar HBV PCR Kit 1.0 11/2012 RealStar HBV PCR Kit 1.0 For research use only! (RUO) Product No.: 201003 96 rxns INS-201000-GB-02 Store at -25 C... -15 C November 2012 altona Diagnostics GmbH Mörkenstraße
More informationHARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs)
HARVESTING AND CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS (hescs) OBJECTIVE: can be cryopreserved in a liquid nitrogen (LN 2 ) freezer for long-term storage. This Standard Operating Procedure (SOP)
More informationCopyright 2009, International Society for Biological and Environmental Repositories (ISBER)
Logistics and Sample Transport: Overview, Recommendations, Validation Protocol International Society for Biological and Environmental Repositories (ISBER) Introduction Logistics and sample transport can
More informationClinical Waste Management & Sharps Injury Prevention. Clinical Skills
Clinical Skills Clinical Waste Management and Sharps Injury Prevention Clinical Skills and Simulation Team Cardiff and Vale Waste Management Team Aims & Outcomes Aims & Outcomes The aim of this module
More informationHuman Umbilical Cord Blood CD34 + Progenitor Cell Care Manual
Human Umbilical Cord Blood CD34 + Progenitor Cell Care Manual INSTRUCTION MANUAL ZBM0065.03 SHIPPING CONDITIONS Human Umbilical Cord Blood CD34+ Progenitor Cells, cryopreserved Cryopreserved human umbilical
More informationCHAPTER 3 COLLECTION PROCEDURES
CHAPTER 3 COLLECTION PROCEDURES Chapter 3 COLLECTION PROCEDURES 3.1 COLLECTION KIT Principle The cord blood collection kit will be carefully assembled by the Cord Blood Bank (CBB) supervisor or his/her
More informationAttachment D Infection Control Policy METHODS OF IMPLEMENTATION AND CONTROL
Attachment D Infection Control Policy METHODS OF IMPLEMENTATION AND CONTROL OSHA requires that the ECP include a schedule and method of implementation for the various requirements of the standard. The
More informationHuman CD4+T Cell Care Manual
Human CD4+T Cell Care Manual INSTRUCTION MANUAL ZBM0067.02 SHIPPING CONDITIONS Human CD4+T Cells, cryopreserved Cryopreserved human CD4+T cells are shipped on dry ice and should be stored in liquid nitrogen
More informationGENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS
ST-PETERSBURG STATE UNIVERSITY THEODOSIUS DOBZHANSKY CENTER FOR GENOME BIOINFORMATICS GENOME RUSSIA PROJECT BLOOD SAMPLES COLLECTION, DNA EXTRACTION AND DNA QUALITY CONTROL PROTOCOLS 2015 The object of
More informationGeneral Information and Guidelines. Submission of blood tubes
General Information and Guidelines Submission of blood tubes Note: Do not submit blood samples in syringes (especially with needles attached). Syringes that are submitted to the lab with the needles attached
More informationAppendix 2: Example Validation Plan
Appendix 2: Example Validation Plan 1. Purpose / Scope This document describes the validation of: Shipper [enter shipper name] for the use in the inter-hospital/laboratory transport of [Enter components
More informationEXPOSURE CONTROL PLAN (sample) 1 Child Care Directors and Employers
EXPOSURE CONTROL PLAN (sample) 1 Child Care Directors and Employers The Model Exposure Control Plan is intended to serve as an employer guide to the OSHA Bloodborne Pathogens standard. A central component
More informationOptimal management of RA patients who require Biologic Therapy. (ORBIT Study) SAMPLING MANUAL
Optimal management of RA patients who require Biologic Therapy (ORBIT Study) SAMPLING MANUAL VERSION (1.0) 2 TABLE OF CONTENTS Page No. 1. CENTRAL LABORATORY - CONTACT NUMBERS... 3 2. TO RE-ORDER SUPPLIES...
More informationab185916 Hi-Fi cdna Synthesis Kit
ab185916 Hi-Fi cdna Synthesis Kit Instructions for Use For cdna synthesis from various RNA samples This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 1
More informationCold Agglutination Titer detecting Cold Reacting Antibodies
Objectives: Cold Agglutination Titer detecting Cold Reacting Antibodies 1. Perform a serial dilution to determine the amount of cold reacting antibody present in a patient specimen with the results obtained
More informationUltraClean Forensic DNA Isolation Kit (Single Prep Format)
UltraClean Forensic DNA Isolation Kit (Single Prep Format) Catalog No. Quantity 14000-10 10 preps 14000-S 1 prep Instruction Manual Please recycle Version: 10302012 1 Table of Contents Introduction...
More informationContent Sheet 5-1: Overview of Sample Management
Content Sheet 5-1: Overview of Management Role in quality management system management is a part of process control, one of the essentials of a quality management system. The quality of the work a laboratory
More informationGenomic DNA Extraction Kit INSTRUCTION MANUAL
Genomic DNA Extraction Kit INSTRUCTION MANUAL Table of Contents Introduction 3 Kit Components 3 Storage Conditions 4 Recommended Equipment and Reagents 4 Introduction to the Protocol 4 General Overview
More informationModulating Glucose Uptake in Skeletal Myotubes:
icell Skeletal Myoblasts Application Protocol Introduction Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis The skeletal muscle is one of the
More informationNimbleGen DNA Methylation Microarrays and Services
NimbleGen DNA Methylation Microarrays and Services Sample Preparation Instructions Outline This protocol describes the process for preparing samples for NimbleGen DNA Methylation microarrays using the
More informationKGI MEDICAL WASTE MANAGEMENT PLAN
1revised 2012 I. Facility Information KGI MEDICAL WASTE MANAGEMENT PLAN a. Contact Person: Barbara Erwin Director Research Operations/Chemical and Biological Laboratory Safety/Chair. 909-607-0160 i. Facility:
More informationQuick Start Contents
Quick Start Guide 1 Quick Start Contents Contact Information.. 3 Create an Account 3 Order Saliva Collection Supplies...3 Collect a Specimen..4 Order a Test..5 Package and Ship Specimens...8 Schedule FedEx
More informationOne Shot TOP10 Competent Cells
USER GUIDE One Shot TOP10 Competent Cells Catalog Numbers C4040-10, C4040-03, C4040-06, C4040-50, and C4040-52 Document Part Number 280126 Publication Number MAN0000633 Revision A.0 For Research Use Only.
More informationFollicle Dermal Papilla Cell
Follicle Dermal Papilla Cell Instruction Manual Product Size Catalog Number Human Follicle Dermal Papilla Cells (HFDPC) 500,000 cryopreserved cells 500,000 proliferating cells C-12071 C-12072 Product Description
More informationHuman Adult Mesothelial Cell Manual
Human Adult Mesothelial Cell Manual INSTRUCTION MANUAL ZBM0025.01 SHIPPING CONDITIONS Human Adult Mesothelial Cells Orders are delivered via Federal Express courier. All US and Canada orders are shipped
More informationRoom Temp: 8 Hr. Room Temp: 8Hr. Room Temp: ASAP. Refrigerated (2-8C): 3D. Room Temp: ASAP. Refrigerated (2-8C): 3D
Diabetes Chem Panel (Glu, Trig, Chol, HDL, LDL, Chol:HDL Ratio, AST, ALT, BUN, Creat, GFR, Na, K, Cl, CO2, Ca) ANMC Lab Test Requirements Updated 4/7/15 Key: Room Temp (20-25C), Refrigerated (2-8C), (-15
More information2. TRansport ARRANGEMENTS... 5 2.1. Flight arrangements... 5 2.2. Ground transport arrangements... 5
World Marrow Donor Association (WMDA) Guidelines for couriers and the transportation of haematopoietic progenitor cells (HPC-BM, apheresis and therapeutic cells- T Cells) 1. COURIERS... 3 1.1. Courier
More informationCell Culture Protocol for Biogelx Peptide Hydrogel 2D and 3D Cell Culture PRO/BGX/001
Protocol (PRO) Cell Culture Protocol for Hydrogel 2D and 3D Cell Culture PRO/BGX/001 Materials Provided Gel solution (stiffness of Gel will vary as per type of product requested), packaged in a glass vial.
More informationQuantiFERON TB Gold In Tube Method (QGIT Test) Manufacturer: Cellestis Inc. (USA) a QIAGEN Company
QuantiFERON TB Gold In Tube Method (QGIT Test) Manufacturer: Cellestis Inc. (USA) a QIAGEN Company P1078 TB APPRISE TB Ante vs. Postpartum Prevention with INH in HIV Seropositive mothers and their Exposed
More informationUCLA Henry Samueli School of Engineering and Applied Science STANDARD OPERATING PROCEDURE MEDICAL WASTE (BIOHAZARD WASTE) MANAGEMENT
UCLA Henry Samueli School of Engineering and Applied Science STANDARD OPERATING PROCEDURE MEDICAL WASTE (BIOHAZARD WASTE) MANAGEMENT PURPOSE The purpose of this document is to specify the procedures used
More informationEquipment Qualification
Equipment Qualification Deborah L. Griffin, BA Laboratory Supervisor, HSCLab Hillman Cancer Center University of Pittsburgh Cancer Institute University of Pittsburgh Medical Center Equipment Qualification
More informationSequencing Library qpcr Quantification Guide
Sequencing Library qpcr Quantification Guide FOR RESEARCH USE ONLY Introduction 3 Quantification Workflow 4 Best Practices 5 Consumables and Equipment 6 Select Control Template 8 Dilute qpcr Control Template
More informationRNA Extraction and Quantification, Reverse Transcription, and Real-time PCR (q-pcr)
RNA Extraction and Quantification, Reverse Transcription, and Real-time Preparation of Samples Cells: o Remove media and wash cells 2X with cold PBS. (2 ml for 6 well plate or 3 ml for 6cm plate) Keep
More informationIgM ELISA. For the quantitative determination of IgM in human serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.
IgM ELISA For the quantitative determination of IgM in human serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calibrator
More informationHealth Care Workers in the Community
Waste Management for Health Care Workers in the Community Adapted from Waste Management for Health Care Workers in the Community with the permission of Capital Health, Regional Public Health. Distributed
More informationHow To Get Your Books From Millennia African University
Millennia Atlantic University Students: YOU MAY RECEIVE YOUR REQUIRED TEXTBOOKS AND COURSE MATERIALS BY VISITING THE SCHOOL S OFFICIAL AT: www.maubookstore.com WHEN ORDERING YOU MAY REFER TO THE ATTTACHED
More informationNo-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer
APPLICATION NOTE Attune NxT Flow Cytometer No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer Introduction Standard methods for isolating and detecting leukocytes
More informationNew York State Department of Health (NYSDOH) Bureau of Immunization Vaccine Storage and Handling Plan
Vaccines need to be properly stored and administered to ensure maximum efficacy and safety. Check yes or no for each item. Implement changes for items marked no. Personnel: 1. We have designated a primary
More informationHow to safely collect blood samples from persons suspected to be infected with highly infectious blood-borne pathogens (e.g.
How to safely collect blood samples from persons suspected to be infected with highly infectious blood-borne pathogens (e.g. Ebola) Step 1: Before entering patient room, assemble all equipment (1 st part)
More informationInstruction Guide for Cleaning, Testing, and Packaging Kendall SCD 700 Sequential Compression System Controller and Tubing Set
Instruction Guide for Cleaning, Testing, and Packaging Kendall SCD 700 Sequential Compression System Controller and Tubing Set TABLE OF CONTENTS Section 1 Instructions for UNUSED Units 2 Confirm Kit Materials
More informationProto col. GoClone Repor ter Construc ts: Sample Protocol for Adherent Cells. Tech support: 877-994-8240. Luciferase Assay System
Luciferase Assay System Proto col GoClone Repor ter Construc ts: Sample Protocol for Adherent Cells LightSwitch Luciferase Assay System GoClone Reporter Assay Workflow Step 1: Seed cells in plate format.
More informationRouting Guide. Ship-Right Solutions. Version 1. Updated: January 20, 2015. Ship-Right Solutions. 165 Pleasant Ave South Portland, ME 04106
Version 1 Updated: January 20, 2015 Ship-Right Solutions Routing Guide Ship-Right Solutions 165 Pleasant Ave South Portland, ME 04106 Phone: 207-321-3500 Fax: 207-321-3501 E-mail: receiving@shiprightsolutions.com
More informationQIAGEN Supplementary Protocol
QIAGEN Supplementary Protocol Isolation of Peripheral Blood Mononuclear Cells (PBMC) and Purification of Total RNA from PBMC Using the RNeasy Micro or Mini Kit This protocol describes how to isolate PBMC
More informationRead Me First: Unpacking Express5800/320Fc System Cabinets
Read Me First: Unpacking Express5800/320Fc System Cabinets NEC Corporation of America 456-01721-000 Notice The information contained in this document is subject to change without notice. UNLESS EXPRESSLY
More informationCarnegie Mellon University s Policy and Procedures for Recombinant and Synthetic Nucleic Acid Materials Spills
Carnegie Mellon University s Policy and Procedures for Recombinant and Synthetic Nucleic Acid Materials Spills Background In accordance with Section IV-B-2-b-(6) of the NIH Guidelines for Research Involving
More informationABSTRACT INTRODUCTION
Performance of the PAXgene Blood DNA Tube for the Collection, Transport, and Storage of Whole Blood and the Purification of DNA Using the QIAsymphony Instrument L. Rainen 1, K. Guenther 2, E. Provencher
More informationRadiation Sciences Nuclear Medicine Technology Program Hot Lab Log Book (Material)
Radiation Sciences Nuclear Medicine Technology Program Hot Lab Log Book (Material) Table of Content Receiving a Radioactive Package Constancy Test Daily Survey Weekly Wipes Accuracy Test Linearity Geometric
More informationJIANGSU CARTMAY INDUSTRIAL CO.,LTD www.labfurniture.asia mail: info@labfurniture.asia
The basic layout, the main functions and instrumentation concept of micro Inspection Division laboratory, 1, Virology Laboratory 1. Functions: for the city to monitor the prevalence of HIV disease, dealing
More informationSentinel Laboratory Guidelines Procedure Adopted: 5/3/2011 Nebraska Public Health Laboratory Version: December 21, 2015
Sentinel Laboratory Guidelines Procedure Adopted: 5/3/2011 Nebraska Public Health Laboratory Version: December 21, 2015 Lincoln/Greater Nebraska Sentinel Laboratories Packaging and Shipping to Nebraska
More informationAptima Specimen Transfer Kit
Aptima Specimen Transfer Kit For Use with ThinPrep and SurePath Liquid Cytology Specimens Intended Use For in vitro diagnostic use. For US export only. The Aptima Specimen Transfer kit is only for use
More informationHow To Store An Acam2000 Vaccine
ACAM2000 Smallpox (Vaccinia) Vaccine, Live Deployment Guidance - Acambis Inc. Revision: October 2008 Information contained in this guidance will also be available through Acambis, in the event that questions,
More informationDot Blot Analysis. Teacher s Guidebook. (Cat. # BE 502) think proteins! think G-Biosciences www.gbiosciences.com
PR110 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Dot Blot Analysis Teacher s Guidebook (Cat. # BE 502) think proteins! think G-Biosciences
More informationCordBank Limited, which supplies processing and services with respect to cord blood. CordBank is referred to in this contract as CordBank or we ;
CORD BLOOD PROCESSING AND STORAGE CONTRACT This contract concerns the processing, storage and care of cord blood taken from your child s umbilical cord. This service will be supplied by CordBank on the
More informationHuman Free Testosterone(F-TESTO) ELISA Kit
Human Free Testosterone(F-TESTO) ELISA Kit Catalog Number. MBS700040 For the quantitative determination of human free testosterone(f-testo) concentrations in serum, plasma. This package insert must be
More informationHow To Use An Enzymatics Spark Dna Sample Prep Kit For Ion Torrent
SPARK DNA Sample Prep Kit Ion Torrent (SPK0002-V08) Frequently Asked Questions Under what circumstances would I use SPARK DNA Sample Prep Kit for Ion Torrent? Enzymatics SPARK DNA Sample Prep Kit for Ion
More informationTHE UNIVERSITY OF MAINE BIOMEDICAL WASTE MANAGEMENT PLAN
THE UNIVERSITY OF MAINE BIOMEDICAL WASTE MANAGEMENT PLAN Department: The University of Maine Safety and Environmental Management Department Page i TABLE OF CONTENTS Section Title Page 1. Purpose... 1 2.
More informationScott & White Institutional Biosafety Committee Compliance Program Biohazardous Material Spill Clean-Up Procedure Policy #IBC.002
I. Purpose Biohazardous material usage on the Scott & White campus is regulated by the Scott & White Institutional Biosafety Committee (IBC). Those investigators choosing to perform research with biohazardous
More informationBloodborne Pathogens
Bloodborne Pathogens Learning Objectives By the end of this section, the participant should be able to: Name 3 bloodborne pathogens Identify potentially contaminated bodily fluids Describe 3 safe work
More information