A Chromosome Study. Karyotype
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1 A Chromosome Study In this activity, you will create a karyotype from a page of mixed chromosomes. Karyotypes are created by matching homologous pairs and numbering them from largest to smallest. Abnormalities, such as extra or deleted chromosomes can then be diagnosed. Pictured chromosomes will be used for this model rather than real chromosomes, but the process is the same for real chromosomes extracted from cell or fetal samples. One karyotype will be created that represents an abnormal karyotype. You will then compare and diagnose the abnormality present in the patient of that karyotype. Karyotype Examine the page. These chromosomes are actually enlarged photographs of what is seen through a microscope. Note that the sex chromosomes have been labeled for you as either X or Y chromosomes. They have been marked this way to indicate these are the sex chromosomes. Cut out each chromosome with scissors, to make it go faster, cut them out as squares rather than trying to cut around the margin of each chromosome. Prepare a karyotype of these chromosomes. A karyotype is a pattern or picture of chromosomes from one cell grouped into pairs and organized by size. --Pair up each of the chromosomes with its homologous pair, use the size and markings on the chromosomes to determine pairs. Temporarily put the two unshaded chromosomes aside. --On a blank paper, arrange the chromosome pairs from largest to smallest, place in four lines (follow the exam in your notes) and number them. You numbers should range from 1(largest) to 22(smallest). Put the sex chromosomes last, this is pair #23. Glue or tape the chromosomes to the paper in the correct order. Sex chromosomes determine the sex of the individual. (These chromosomes are unshaded on your karyotype). Finding incorrect chromosomes numbers in human somatic cells of an unborn baby alerts doctors to the fact that their child is abnormal and will be born with birth defects. *If the unborn has an extra number 13 chromosome, it it born with Patau syndrome. An extra chromosome 18 results in Edward syndrome. An extra chromosome number 21 results in Down Syndrome. A missing sex chromosome results in an X0 offspring who has Turner's syndrome. An extra X chromosome results in Klinefelters syndrome (XXY).
2 Karyotype Questions: --Examine the page marked "Abnormal". Look at the top corner, what Set do you have? 1. How many chromosomes are present in the abnormal karyotype: 2. How many chromosomes are present in each cell of this abnormal karyotype: 3. Which chromosome pair is abnormal? 4. What syndrome does this unborn child within this abnormal karyotype have? 5. What sex will the unborn child be within this abnormal karyotype? Analysis: 1. Describe two types of information that can be gained about a child before it is born through a karyotype. 2. Would the diagnosis of Turner s Syndrome in a single cell necessarily mean that every cell of the body would contain 45 chromosomes? Explain your answer? 3. Is it possible for two people who have Down s syndrome to give birth to a normal child? Explain your answer? 4. Would it be possible to produce a baby who has 48 chromosomes? Explain your answer?
3 5. More males than females suffer from colour blindness. Speculate as to why females who have Turner s syndrome have a similar incidence of colour blindness as males. 6. Who are the individuals that should decide whether screening for a genetic disease such as Down s syndrome takes place? What considerations should be involved in the decision e.g., medical, social, financial, ethical etc? Do you think that prenatal screen should be compulsory for everyone, e.g. mothers with increased risk of genetic conditions?
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10 Teacher's Guide for "A Chromosome Study" This activity requires students to cut and paste paper models of chromosomes in order of largest to smallest. The chromosomes must be paired with their homologs. Students will often have trouble finding pairs and getting the exact matches and size orientation. Generally, when grading I look to see if it look mostly right, and look for the specific abnormality showing an extra chromosome. Sheet A - Down Syndrome Patient A ( Click on the link to "Complete Patient A's Karyotype" ) --Examine the page marked "Abnormal". Look at the top corner, what Set do you have? 7. How many chromosomes are present in the abnormal karyotype: 8. What is the diploid chromosome number for this abnormal karyotype: 9. Which chromosome pair is abnormal? 10. What syndrome does this unborn child within this abnormal karyotype have? 11. What sex will the unborn child be within this abnormal karyotype?
11 Sheet B - Klinefelter Syndrome (XXY) Patient B - click on the link to go to Patient B and repeat the above process. --Examine the page marked "Abnormal". Look at the top corner, what Set do you have? 7. How many chromosomes are present in the abnormal karyotype: 8. What is the diploid chromosome number for this abnormal karyotype: 9. Which chromosome pair is abnormal? 10. What syndrome does this unborn child within this abnormal karyotype have? 11. What sex will the unborn child be within this abnormal karyotype?
12 Sheet C - Edward Syndrome (extra chromosome 18) Patient C - click on the link to go to Patient C and repeat the above process. --Examine the page marked "Abnormal". Look at the top corner, what Set do you have? 7. How many chromosomes are present in the abnormal karyotype: 8. What is the diploid chromosome number for this abnormal karyotype: 9. Which chromosome pair is abnormal? 10. What syndrome does this unborn child within this abnormal karyotype have? 11. What sex will the unborn child be within this abnormal karyotype?
13 Sheet D - Patau Syndrome (extra chromosome 13) --Examine the page marked "Abnormal". Look at the top corner, what Set do you have? D_ 7. How many chromosomes are present in the abnormal karyotype: What is the diploid chromosome number for this abnormal karyotype: _47_ 9. Which chromosome pair is abnormal? What syndrome does this unborn child within this abnormal karyotype have? Trisomy What sex will the unborn child be within this abnormal karyotype?
Each person normally has 23 pairs of chromosomes, or 46 in all. We inherit one chromosome per pair from our mother and one from our father.
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