NIS (Na+/I- Sympoter) TPO
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2 Production of Thyroid Hormones NIS (Na+/I- Sympoter) TPO
3 Thyroid gland secretes tri and tetra iodo thyronine also called as T3 and T4. Derived from tyrosine on de-iodination. Functions of thyroid hormones. Necessary for proper functioning of almost all cells in our body and for biological processes. Calorigenic effect or thermogenesis. T4 stimulates RNA synthesis and thereby protein synthesis.
4 B. Functions of thyroid hormones I. Increase BMR ( Basal Metabolic Rate ) cellular metabolic activity by : size, total membrane surface & number of mitochondria ATP formation active transport of ions ( Na +, K + ) II. Promote growth & development of the brain during fetal life and for the first few years of postnatal life.
5 Transport of thyroid hormones T4 and T3 are transported in blood by binding to plasma proteins called thyroxine binding globulin. The bound form is biologically inactive.and FREE form is active. Thyroxine binding globulin carries 80% of T4 and 60% of T3.
6 Regulation of thyroid hormones. TSH secretions are controlled by Circulating levels of thyroid hormones. Thyrotropin releasing hormones. Hypothalamic pituitary thyroid hormone axis is involved in feed back control.
7 ASSESSMENT OF THYROID FUNCTION. Measurement of T4 and T3 by ELISA. Hyper thyroidism both T4 and T3 are increased and TSH is decreased. Hypothyroidism botht4 and T3 are decreased and TSH is increased due to feed back inhibition. When hypothyroidism is due to hypothalamic or pitutary causes TSH,T4 and T3 are all decreased.
8 Normal T4 and T3 values. T4 = 5 to 12.5 μgm/dl. T3 =70 to200 η gm/dl. Estimation of free serum T3 and T4 : Free serum T3andT4 in blood not bound to proteins is independent of changes in(tbg ). It provides more reliable diagnosis of thyroid dysfunction than measurement of total T4and T3.
9 Free T4 = pmol/l Free T3= 3 9 pmol/l Clinical interpretation: values are assosciated with hyper thyroidism and thyrotoxicosis. Hypothyroidism. Thyroxin binding globulin: Almost all thyroid hormones are bound to protein, TBG. TBG conc affects total plasma conc of T4and T3. Normal values of TBG 12 28micro gm/dl.
10 TBG level is increased in: - Hypothyroidism - Pregnancy - Oestrogen therapy TBG level is decresed in : - Hyperthyroidism -Nephrotic syndrome - Liver disease.
11 Resin Uptake Test: Labelled hormone 125 I T 3 is used. T 3 uptake is % of labelled hormone taken up by resin and is inversely related to unoccupied binding sites ontbg. Serum Thyroid stimulating hormone(tsh) Most sensitive,specific and reliable test of thyroid status. Anterior pitutary TSH release of stored thyroid hormones.
12 Normal values of serum TSH 2 6 micro units/ml. Clinical interpretation: Increased levels are seen in primary hypothyroidism due to absence of negetaive feed back control on pitutary. Decreased levels are seen in Primary(thyroid gland failure) Secondary (anterior pitutary failure) Tertiary (hypothalamic failure).
13 THYROID ANTIBODIES Measuring thyroid antibodies helps to demonstrate presence of auto immune disorders. Thyroid peroxidase and thyroglobulins - Hashimato s thyroiditis and Grave s disease.
14 Thyroid scanning : Ultra sound sonography CT scan MRI scan For the presence of thyroid mass,cysts and solid tumours.
15 Hyperthyroidism (Toxic Goitor, Thyrotoxicosis, Graves disease ) Causes : hyperplasia long-acting thyroid stimulator (LATS) ( globulin antibody ) Symptoms : intolerance to heat increase sweating diarrhea muscle weakness nervousness psychic disorders extreme fatigue tremor of the hand Exophthalmos ( protrusion of the eyeballs ) : edematous swelling of the retro-orbital tissues and degeneration of the extraocular muscle
16 Diagnosis (1) free thyroxine (2) BMR (3) rate of uptake of iodine (4) PBI ( protein binding iodine ) (5) TPO Ab, Tg AB, TSH-R[stim] Ab, TSH-R[block] Ab Treatment (1) surgical removal of the thyroid gland (2) antithyroid drug: propylthiouracil
17 Hypothyroidism Causes : endemic goiters idiopathic nontoxic colloid goiter Symptoms : extreme somnolence ( hrs/day ) cretinism ( infant or childhood ) depressed growth of hair & scaliness of the skin muscle sluggishness constipation slow heart rate mental sluggishness cardiac output frog-like husky voice blood volume arterosclerosis body weight myxedema Diagnosis : same as hyperthyroidism Treatment : thyroid extract
18 Goiter Low T 3 & T 4 Negative feedback Excess TSH
19
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