BD FACSCanto II. Tel :
|
|
- Darren Fowler
- 7 years ago
- Views:
Transcription
1 BD FACSCanto II Tel :
2 BD FACSCanto II BD FACSCanto 03 BD FACSCanto 13 Surface Markers.. 22 DNA Analysis FACSDiva
3 BD FACSCanto II BD FACSCanto II 488nm 20mw 633nm 17mw 6~ % 120 ul/min 10,000 events/sec BD FACSCanto II 3
4 1.1 BD FACSCanto II BD FACStation FACSCanto II 4
5 1.2 Fluidics Sample injection tube (SIT) aspirator arm flow cell SIT SIT flow cell tubing SIT aspirator arm aspirator arm bar SIT tube guide SIT (Loader ) tube eject cylinder (Loader ) tube sensor SIT bal seal access Bal seal BD FACSCanto II 5
6 SIT SIT SIT SIT BD FACSDiva SIT Acquisition Dashboard SIT Flush Checkbox SIT FACSCalibur 3 ml Flow Cell 6
7 1.3 Optics BD FACSCanto II Excitation Optics 1-3 FACSCanto II BD FACSCanto II nm mw Coherent R Sapphire TM FITC, PE, PE-Texas Red, PerCP, PerCP-Cy5.5, PE-Cy7,PI JDS Uniphase TM HeNe APC,APC-Cy7 ( ) BD FACSCanto II 7
8 1.3.2 Collection Optics ( 1-3) 4-2 FACSCanto ( 4-2 ) Longpass DM PMT PMT PMT bandpass Blue A 750~810 nmblue B 670~730nmBlue D 564~606nmBlue E 515~545nmBlue F 488nm 8
9 photodiode ( 1-2) ( 4-2 ) PMT Position LP Filter nm BP or LP Filters Fluorochromes nm 488 Laser Blue A: /60 PE-Cy7 Blue B: LP PerCP, PerCP-Cy5.5 Blue C: 610 Blank filter N.A Blue D: /42 PE Blue E: /30 FITC Blue F: Blank filter 488/10 SSC 633 Laser Red A: /60 APC-Cy7 Red B: 685 Blank filter N.A Red C: Blank filter 660/20 APC BD FACSCanto II 9
10 1.4 Electronics PMT BD FACSCanto II BD FACSCanto User s Guide Signal detection Power Panel Port or Button System Power FACSCanto II Power Out FACSCanto II Communication cable System AC power plug System circuit breaker 10
11 1.4.2 Acquisition Indicator Lights BD FACSCanto II 11
12 FACSFlow 10 5 FACS Shutdown 5 FACSClean BD FACSCanto BD FACSDiva 12
13 1.5.2 FACSCanto II Off Circuit Breaker On Powering Off Condensation Trap 60~70psi 50psi BD ( ) BD FACSCanto II 13
14 BD FACSCanto II BD FACSCanto II FACSCanto II BD FACSCanto II Clinical or FACSDiva BD PC Pentium R IV 1GB RAM 40GB Windows XP Professional (US-English) 17 BD FACSCanto II Clinical Software version 2.1 or above Instrument QC, Lymphocyte subset TBNK, HLA-B27, Stem Cell Enumeration BD FACSDiva version 5.0 or above BD FACSDiva Data Manager 14
15 Cytometer > Fluidics Startup OK BD FACSCanto II BDIS ( )Enter BD FACSDiva 5. Instrument 6. Instrument Instrument Connected Instrument FACSFlow Instrument Disconnected FACSDiva 7. : Shutdown solution Sheath buffer System Prime -- Bubble filter purge Degas flow cell 5~10 BD FACSCanto II 15
16 Instrument > Automatic Cleaning FACSDiva FACSDiva Automatic Clean Automatic Clean 8. OK : For Diva Instrument Remaining warmup time 7:27 The system is ready
17 2.2 (Controls in Instrument Frame) Fluid Level Indicators BD FACSDiva FACSFlow 17% 34% 51% 68% shutdown cleaning 20% FACSFlow FACSFlow ( ) ( ) FACSFlow <17% >83% FACSFlow 0% >99% Laser Tab Window Extension FSC Area Scaling BD FACSCanto II 17
18 Status Tab 2.3 Acquisition Dashboard Next Tube Acquire Data Record Data list mode data Restart Acquire Record SIT Flush SIT 18
19 Flow Rate Low 10 µl /min Medium 60 µl /min High: 120 µl /min Fluidics Startup 2. 55~65 Psi 3. 1X10 6 cells/ml 0.5 ml 4. FALCON Fluidics Shutdown BD FACS Shutdown solution 1. 3 ml FACSClean 10%Bleach 2. Basic controls Acquire Data 3. High Flow Rate Acquire 5 5 Acquire 4. FACSClean 3 ml dh2o BD FACSCanto II 19
20 5. Acquisition controls Acquire High Flow Rate Acquire Acquire Fluidics Shutdown 7. Cytometer > Fluidics Shutdown OK OK Automatic Cleaning FACSDiva Fluidics Shutdown 1 ml SIT 8. dh2o 9. 1/ Condensation Trap 20
21 2.6 FACSCanto II Clean Flow Cell.. Instrument/Clean Modes/Clean Flow Cell.. 3 ml FACSClean FACSClean fluidics startup fluidics shutdown BD FACSCanto II 21
22 De-Gas or Bubble Filter Purge CV Instrument > Cleaning Modes > De-gas Flow Cell.. Instrument > Cleaning Modes > Bubble Filter Purge.. Priming Instrument > Cleaning Modes > Prime After Tank Refill.. OK (Long Clean) Long Clean FACSClean FACS shutdown 75 FACSClean >275 ml >1,100 ml Instrument > Cleaning Modes > Long Clean
23 2.7 FACSCanto II BD FACS 7-color setup beads Cat. No Rainbow fluorescent particles Cat. No Nile Red fluorescent particle Cat. No BD FACSFlow sheath fluid Cat. No BD FACS cleaning solution Cat. No BD FACS shutdown solution* Cat. No House hold bleach for waste 5mL polystyrene test tubes (BD Falcon ) uncapped, 125 per bag BD Cat. No capped, 125 per bag BD Cat. No capped, 25 per bag BD Cat. No with cell-strainer cap BD Cat. No *BD FACS shutdown solution BD FACSCanto II 23
24 Surface Markers BD FACSDivia Experiment Global Sheet 24
25 Surface Markers Instrument Setting ( ) 3.1 BD FACSDivia 1 2 BD FACSDivia 3 View View>>Reset positions Browser ( ) >> >> >> (Folder>>Experiment>>Specimen>>Tube BD FACSCanto II 25
26 5 Folder Folder 6 Experiment Folder Experiment Experiment>>New Experiment Experiment templates Blank Experiment with Sample Tube 7 FACSDiva Blank Experiment with Pop-up Menu 2-color exp Enter 26
27 8 Blank Experiment with Sample Tube Specimen_001 Specimen Specimen Specimen Tube_001 Specimen_001 Tube color exp Instr Settings ( ) Inspector Parameters Parameters PerCP-Cy5-5 PE-Cy7 APC APC-Cy7 FITC PE Parameters Add or Delete FITC EGFP Alexa 488 PerCP-Cy5-5 PerCP PI 3.4 Global Sheet BD FACSCanto II 27
28 10 Global Sheet1 Dot Plot 1 FSC-A vs SSC-A 2 FITC-A vs PE-A 3.5 Optimize Settings FSC SSC Surface Markers CD3 FITC 3 CD4 PE FSC SSC 11 Instument Tube_ Basic Controls Acquire Record 28
29 FSC SSC Voltage Instrument Parameters FSC SSC FSC SSC Acquire 13 Basic Controls Acquire Instrument Threshold FSC 14 Global Sheet1 FSC-A vs SSC-A P1 P1 BD FACSCanto II 29
30 15 Shift Show Population P1 P1 Instument Voltage 16 Tube_001Basic Controls Acquire Record Instrument Parameter Acquisition Controls Acquire FITC-A vs PE-A quadrants 30
31 17 FITC Tube_001 Basic Controls Acquire PE-%FITC unstained(q3) FITC alone(q4) PE-A Mean 18 PE Tube_001 Basic Controls Acquire FITC-%PE unstained(q3) PE alone(q1) FITC-A Mean BD FACSCanto II 31
32 19 Experiment Instr Settings Inpector Print Typical Instrument Setting for 2-color experiment on FACSCanto II Specimen_001_Tube_001 Specimen_001 Inspector Sample_0831 Enter 32
33 21 Tube_001 Inspector Name CD3_CD4_001 Enter Diva 22 Labels Lable ( ) FITC Label CD3 23 Lable ( ) Events to record Stopping gate Storage gete Global sheet1 FSC/SSC P1 populations marker quadrants 24 CD3 FITC/CD4 PE Sample_001 CD3_CD4_001 Acquisition Controls Acquire P1 Acquisition Controls Record BD FACSCanto II 33
34 25 10, Acquisition Controls Next Tube Tube CD3_CD4_002 CD3_CD4_002 CD3 FITC/CD4 PE Acquisition Controls Acquire P1 Record 26 34
35 DNA Analysis DNA BD FACSDivia DNA Analysis Global Sheet ModFit LT BD FACSCanto II 35
36 DNA Instrument Setting ( ) 4.1 BD FACSDivia 1 2 BD FACSDivia For Diva 4.X.X or 5.X.X : Instrument>Instrument Configuration DNA PI DAPI Duplicate DNA_001 OK Parameter Add PI Blue (Laser) D(Detector) OK Set 36
37 3.2.1 For Diva 6.0 or above : Cytometer>View Configurations DNA PI DAPI Configuration Duplicate DNA_ Edi Ctrl Parameters PE PI Blue D Set Configuration OK 4.2 DNA Analysis 7 Folder Folder 8 Experiment Folder Experiment Experiment>>New Experiment Experiment templates Blank Experiment with Sample Tube BD FACSCanto II 37
38 9 FACSDiva Blank Experiment with Inspector DNA_001 Enter 10 DNA_001 Specimen_001 Specimen Specimen Specimen Tube_001 Specimen_001 Tube DNA_001 Instr Settings ( ) Inspector Parameters Parameters PerCP-Cy5-5 PE-Cy7 APC APC-Cy7 FITC PE 12 Parameters Add or Delete PE P 38
39 13 PI LIN PI-W 4.4 Global Sheet 14 Global Sheet1 Dot Plot 1 FSC-A vs SSC-A 2 PI-W vs PI-A Histogram 3 PI-A histogram BD FACSCanto II 39
40 4.5 Optimize Settings PI=5,000 DNA FSC SSC optional DNA Unstimulated PI Stimulated Positive PI PI 15 Tube_001 Instrument Threshold PI PI 5, DNA Low 40
41 17 Basic Controls Acquire Record PI Voltage G0 PI-A 50 X Global Sheet1 PI-W vs PI-A Single Cells P1 P1 FSC SSC 19 Instrument Parameters FSC SSC FSC SSC FSC SSC Voltage Jurkat Acquire BD FACSCanto II 41
42 20 PI-A Show Population P1 PI-A P1Jurkat 21 Basic Controls Acquire Instrument Threshold PI 22 DNA Experiment Instr Settings Inpector Print 42
43 Specimen_001_Tube_001 Specimen_001 Inspector Sample_0831 Ente 26 Tube_001 Inspector Name PI_001 Enter Diva 27 Labels Experiment>Experiment Layout Experiment Layout Lable ( ) 28 Experiment Layout Acquisition ( ) PI_001 Events to Record 20,000 OK BD FACSCanto II 43
44 29 Acquisition Controls Low Stopping gate Storage gete 30 Global sheet1 PI-W/PI-A P1 P1 31 PI Sample_001 PI_001 Acquisition Controls Acquire P1 Acquisition Controls Record 20, Acquisition Controls Next Tube Tube PI_002 PI_002 Acquisition Controls Acquire P1 Record 33 44
45 DNA ModFit LT Cell Cycle DNA_001 File>>Export>>FCS files 35 FCS2.0 BD FACSCanto II 45
46 36 Cell Cycle G0/1-S-G2/M ModFit LT 46
47 FACSDiva FACSDiva BD FACSDvia Global sheet BDISEnter BD FACSDiva Experiment>>New Experiment Experiment templates Blank Experiment BD FACSCanto II 47
48 2 File>>Import>>FCS Files D:/BD EXPORT/EXPERIMENT/6 Color cocktails Global Worksheet 3 New Global Worksheet Global Sheet2 Analysis 48
49 4 Analysis 1 FSC-A vs SSC-A 2 FITC-A vs PE-A 5 tube_001 Analysis FSC-A X FSC-H BD FACSCanto II 49
50 6 snap-to-gate FSC-A SSC-A Diva 7 FITC-A PE-A Show Populations P1 8 FITC-A PE-A P1 50
51 9 Create statistics View 10 Edit Statistics View 11 P1 Q1~4 BD FACSCanto II 51
52 12 Statistics Analysis File >> Print CSV File >> Export >> statistics 52
53 15 test1 Save Shift 17 File >> Export >> Worksheet Elements 18 Expor BD FACSCanto II 53
54 P Color LnL Inspector Analysis 21 6 Color LnL Batch Analysis Print Statistics START 54
55 22 test 1 Save BD FACSCanto II 55
56 5.7 Log Out 23 - FACSDiva 24 File >> Quit File >> Log Out Quit 5.8 Excel 25 Excel >> Microsoft Excel 26 D:\BD Export\Statistic CSV 56
57 27 30 BD FACSCanto II 57
58 (PMTs 4-2 ) (nm) (nm) Fluorescein, FITC (Blue E) Phycoerythrin-R, PE (Blue D) Peridinin-chlorophyll, (Blue B) PerCP PerCP-Cy (Blue B) Cy-Chrome (Blue B) PE-Texas Red (B or D) PE-Cy (Blue B) PE-Cy (Blue A) Allophycocyanine (Red C) APC-Cy (Red A) Ethidium Bromide 510 (+ds DNA) 595 (Blue D) Propidium Iodide 536 (+ds DNA) 623 (B or D) Acridine Orange 480 (+ DNA) (+ RNA) 520 (Blue E) 650 (Blue B) Thiazole Orange 509 (+ RNA) 533 (Blue E) Viability Propidium Iodide (B or D) YOPRO (Blue E) Acridine Orange (Blue E) 7-AAD (Blue B) DiO-C6 (3) (Blue E) Mitochondrial Rhodamine (Blue E) ph BCECF-AM 488 Ratio 520/620 SNARF1-AM 514 Ratio 587/640 Fluo4-AM (Blue E) Calcium Green (Blue E) Fura Red (Blue B) H2O2 sensitive Dihydrorhodamine (Blue E) DCFH-DA (Blue E) O2- radical sensitive Hydroethidine (Blue D) Esterase sensitive Fluorescein -DA (Blue E) 58
59 Optical Configurations nm mw Coherent R Sapphire TM FITC, PE, PE-Texas Red, PerCP, PerCP-Cy5.5, PE-Cy7, PI JDS Uniphase TM HeNe APC, APC-Cy7 Point Source iflex Pacific Blue, AmCyan ( ) PMT Position LP Filter nm BP or LP Filters Fluorochromes nm 488 Laser Blue A: /60 PE-Cy7 Blue B: LP PerCP, PerCP-Cy5.5 Blue C: 610 Blank filter N.A Blue D: /42 PE Blue E: /30 FITC Blue F: Blank filter 488/10 SSC 633 Laser Red A: /60 APC-Cy7 Red B: 685 Blank filter N.A Red C: Blank filter 660/20 APC 405 Laser Viiolet A: /50 AmCyan Violet B: Blank filter 450/50 Pacific blue BD FACSCanto II 59
60 ( 5-3 ) PMT Position LP Filter nm BP or LP Filters Fluorochromes nm 488 Laser Blue A: /60 PE-Cy7 Blue B: LP PerCP, PerCP-Cy5.5 Blue C: /23 PE-Texas Red Blue D: /42 PE Blue E: /30 FITC Blue F: Blank filter 488/10 SSC 633 Laser Red A: /60 APC-Cy7 Red B: /21 Alexa Fluor-700 Red C: Blank filter 660/20 APC 60
FACSCanto RUO Special Order QUICK REFERENCE GUIDE
FACSCanto RUO Special Order QUICK REFERENCE GUIDE INSTRUMENT: 1. The computer is left on at all times. Note: If not Username: Administrator Password: BDIS 2. Unlock the screen with your PPMS account (UTSW
More informationGETTING STARTED. 6. Click the New Specimen icon.
BD FACSDiva Software Basic Experiment Guide Please note, this guide is not intended to take the place of in-person training but is to be used as a reference as needed after completing training with Unified
More informationHow to operate the BD FACSCanto flow cytometer
How to operate the BD FACSCanto flow cytometer Preface Dear colleague, the BD FACSCanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells.
More informationBD FACSDiva 4.1 - TUTORIAL TSRI FLOW CYTOMETRY CORE FACILITY
BD FACSDiva 4.1 - TUTORIAL TSRI FLOW CYTOMETRY CORE FACILITY IMPORTANT NOTES BEFORE READING THIS TUTORIAL This is a very expensive piece of equipment so PLEASE treat it with respect! After you are done
More informationUCHC Guide to FACS DiVa. LSR II instruments
UCHC Guide to FACS DiVa LSR II instruments Start Up: Turn on the instrument using the Green power button. Fluidics pump power, LSR II-B & C LSR II-B and LSRII-C-> Turn on the fluidics pump and ensure that
More informationCyAn : 11 Parameter Desktop Flow Cytometer
CyAn : 11 Parameter Desktop Flow Cytometer Cyan ADP 3 excitation lines 488nm, 635nm, and UV or violet 11 simultaneous parameters FSC, SSC, and 7-9 colors with simultaneous width, peak, area, and log on
More informationBD LSR II and FACSDiVa Software. Dr. Jens Fleischer, Basel Dr. Norbert Leclere,, Berlin
BD LSR II and FACSDiVa Software, Basel Dr. Norbert Leclere,, Berlin Overview Electronics Covers Fluidics Connectors Controlpanel Sample Port On/Off The Control Panel Ease of Use The basic procedures to
More informationBD FACSCanto II Instructions For Use
BD FACSCanto II Instructions For Use IVD For In Vitro Diagnostic Use bdbiosciences.com Part No. 642239 Rev. A June 2007 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax
More informationBD FACSCanto II Flow Cytometer Reference Manual
BD FACSCanto II Flow Cytometer Reference Manual For In Vitro Diagnostic Use bdbiosciences.com Part No. 640806 Rev. A May 2006 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995
More informationpowerful. Intuitive. Customizable. The new star of benchtop flow cytometry
powerful. Intuitive. Customizable. The new star of benchtop flow cytometry About ACEA Established in 2002, ACEA Biosciences, Inc. develops cutting-edge cell analysis platforms for life science research.
More informationAnalyzer Experiment setup guide for LSRII and Canto s
Analyzer Experiment setup guide for LSRII and Canto s 1. Check the instrument configuration on our website to determine the most appropriate cytometer for your experiment, https://depts.washington.edu/flowlab/instrumentation.html,
More informationTechnical Bulletin. Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva Version 6 Software. Abstract
arch 212 Standardizing Application Setup Across ultiple Flow Cytometers Using BD FACSDiva Version 6 Ellen einelt, ervi Reunanen, ark Edinger, aria Jaimes, Alan Stall, Dennis Sasaki, Joe Trotter Contents
More informationKatharina Lückerath (AG Dr. Martin Zörnig) adapted from Dr. Jörg Hildmann BD Biosciences,Customer Service
Introduction into Flow Cytometry Katharina Lückerath (AG Dr. Martin Zörnig) adapted from Dr. Jörg Hildmann BD Biosciences,Customer Service How does a FACS look like? FACSCalibur FACScan What is Flow Cytometry?
More informationInstitut für Experimentelle Immunologie und Bildgebung
Page 1 von 6 Starting Up the System Start up the computer, start Windows and log in as IMCES_user start BD FACSDiva software with your Groupname Turn on the cytometer main power Wait a moment and Check
More informationBD FACSComp Software Tutorial
BD FACSComp Software Tutorial This tutorial guides you through a BD FACSComp software lyse/no-wash assay setup run. If you are already familiar with previous versions of BD FACSComp software on Mac OS
More informationBD FACSCanto Flow Cytometer. Efficient Easy Powerful
BD FACSCanto Flow Cytometer Efficient Easy Powerful The BD FACSCanto System: Optimal Workflow and Time Management Automated compensation algorithms with optional off-line compensation, streamlined sample
More informationLSR II Users Guide Stanford Shared FACS Facility 06/10/13
LSR II Users Guide Stanford Shared FACS Facility 06/10/13 A configuration sheet is located on the Website in the Instrument Overview area Do I need to run beads when I collect data using Diva Digital?
More informationIntroduction to flow cytometry
Introduction to flow cytometry Flow cytometry is a popular laser-based technology. Discover more with our introduction to flow cytometry. Flow cytometry is now a widely used method for analyzing the expression
More informationApplication Note. Selecting Reagents for Multicolor Flow Cytometry. Holden Maecker and Joe Trotter BD Biosciences, San Jose
Selecting Reagents for Multicolor Flow Cytometry Holden Maecker and Joe Trotter BD Biosciences, San Jose Contents 1 The basics: Know your instrument 2 Fluorochromes: Go for the bright 3 Colors and specificities:
More informationUsing BD FACSDiva CST To. Evaluate Cytometer Performance, Create Custom Assay Settings. and
Using BD FACSDiva CST To Evaluate Cytometer Performance, Create Custom Assay Settings and Implement Cross-Instrument and Cross-Site Standardization of Assays PART 2 Alan M. Stall Director, Advanced Cytometry
More informationFlow Cytometry. flow cytometer DNA apoptosis ph
Flow Cytometry flow cytometer DNA apoptosis ph flow cytometry flow cytometer (a) (b) cell counting instrument (c) 1960 ink-jet technology 17 19 1940 1950 fluorescence microscopy fluorescent dye DNA polyclonal
More information1. Instrument Layout pg 2. 2. Summit Overview pg 3. 3. Data Storage and Data Files pgs 6-7. 4. Saving Data pg 7. 5. Start Up pg 8
Table of Contents 1. Instrument Layout pg 2 2. Summit Overview pg 3 3. Data Storage and Data Files pgs 6-7 4. Saving Data pg 7 5. Start Up pg 8 6. Choosing and Naming Parameters pg 9 7. Creating Histograms
More informationBoundary-breaking acoustic focusing cytometry
Boundary-breaking acoustic focusing cytometry Introducing the Attune NxT Acoustic Focusing Cytometer a high-performance system that s flexible enough for any lab One of the main projects in my laboratory
More informationMulticolor Flow Cytometry: Setup and Optimization on the BD Accuri C6 Flow Cytometer
Multicolor Flow Cytometry: Setup and Optimization on the BD Accuri C6 Flow Cytometer Presented by Clare Rogers, MS Senior Marketing Applications Specialist BD Biosciences 23-13660-00 Webinar Overview Multicolor
More informationBD FC Beads 8-Color Kit for BD OneFlow Assays
BD FC Beads 8-Color Kit for BD OneFlow Assays 7/2014 23-15785-00 IVD 5 tests per kit Catalog No. 658621 BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. 2014 BD Becton,
More informationUsing the BD TM Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking
Using the BD TM Cytometer Setup and Tracking (CS&T) System for Instrument Characterization and Performance Tracking Mark KuKuruga Senior Technical Applications Specialist BD Biosciences 23-14462-00 Outline
More informationThese particles have something in common
These particles have something in common Blood cells Chromosomes Algae Protozoa Certain parameters of these particles can be measured with a flow cytometer Which parameters can be measured? the relative
More informationCell Cycle Tutorial. Contents
Cell Cycle Tutorial Contents Experimental Requirements...2 DNA Dyes...2 Protocols...3 PI Parameter & Analysis Setup...4 PI Voltage Adjustments...6 7-AAD Parameter Setup...6 To-Pro3 Parameter Setup...6
More informationFACS Laboratory. BD LSRs Operator Training. Training Guide. http://london-research-institute.org.uk/technologies/facs
FACS Laboratory facs_lab@cancer.org.uk Training Guide http://london-research-institute.org.uk/technologies/facs BD LSRs Operator Training LRI FACS lab 1 Components 1.1 Basic parts overview.pg3-6 Overview
More informationCyFlow SL. Microbiology. Detection and Analysis of Microorganisms and Small Particles
CyFlow SL Microbiology Detection and Analysis of Microorganisms and Small Particles 01 COMPANY Flow Cytometry made in Germany New sophisticated applications and increasing requirements for reliable results
More informationGetting Started with BD FACSDiva Software
Getting Started with BD FACSDiva Software For In Vitro Diagnostic Use bdbiosciences.com Part No. 642213 Rev. A June 2007 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax
More informationScience is hard. Flow cytometry should be easy.
Science is hard. Flow cytometry should be easy. TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE... 1 1.1 Starting BD Accuri C6 Software... 1 1.2 BD Accuri C6 Software Workspace... 2 1.3 Opening
More informationEstablishing Optimum Baseline PMT Gains to Maximize Resolution on BD Biosciences Digital Flow Cytometers
A P P L I C A T I O N N O T E BD Digital Flow Cytometers Establishing Optimum Baseline PMT Gains to Maximize Resolution on BD Biosciences Digital Flow Cytometers Specific Aims This application note describes
More informationIntroduction to Flow Cytometry
Outline Introduction to Flow Cytometry Basic Concept of Flow Cytometry Introduction to Instrument Subsystems Daisy Kuo Assistant Product Manager E-mail: daisy_kuo@bd.com BDBiosciences Application Examples
More informationCyFlow SL. Healthcare Immunology. Portable FCM System for 3-colour Immunophenotyping
CyFlow SL Healthcare Immunology Portable FCM System for 3-colour Immunophenotyping 01 COMPANY Flow Cytometry made in Germany New sophisticated applications and increasing requirements for reliable results
More informationIntroduction to Flow Cytometry -- BD LSR II. What is Flow Cytometry? What Can a Flow Cytometer Tell Us About a Cell? Particle Size
Introduction to Flow Cytometry -- BD LSR II Daisy Kuo 郭 正 佼 Assistant Product Manager BD Biosciences Daisy_kuo@bd.com What is Flow Cytometry? Flow = Fluid Cyto = Cell Metry = Measurement A variety of measurements
More informationUCHC Guide to CellQuest Pro. FACS Calibur instruments
UCHC Guide to CellQuest Pro FACS Calibur instruments Startup: Turn on Cytometer using green switch on the side of the cytometer Cytometer Power Switch Within a minute of turning on the cytometer; turn
More informationInstrument Characterization and Performance Tracking for Digital Flow Cytometers
Instrument Characterization and Performance Tracking for Digital Flow Cytometers BD Biosciences Cytometer Set-up & Tracking (CS&T) System For Research Use Only. Not for use in diagnostic or therapeutic
More informationFlowSight. Flow cytometry with vision
FlowSight Flow cytometry with vision Flow cytometry with vision Introducing FlowSight Capable: Sensitive and flexible for every need Intuitive: Easy-to-use, with imagery for every cell Affordable: Designed
More informationFACS-CALIBUR. USER-Guide
FACS Calibur User guide Version 2.1-03/2006 AXS 1 FACS-CALIBUR Version 2.1 USER-Guide Note: This guide does note compensate for a personal introduction from an operator before first usage Draft mainly
More informationHIGH PERFORMANCE FLOW CYTOMETER FOR EVERYONE
POWERFUL. INTUITIVE. CUSTOMIZABLE. HIGH PERFORMANCE FLOW CYTOMETER FOR EVERYONE ABOUT ACEA Established in 2002, ACEA Biosciences, Inc. develops cutting-edge cell analysis platforms for life science research.
More informationBecton-Dickinson FACSCalibur Flow Cytometer Instructions
Start-up 1. Turn on flow cytometer by pushing green button on right side of instrument, toward the back. Give it 30 minutes to warm up before collecting data. 2. Turn on monitor. 3. Turn on computer. If
More informationCOMPENSATION MIT Flow Cytometry Core Facility
COMPENSATION MIT Flow Cytometry Core Facility Why do we need compensation? 1) Because the long emission spectrum tail of dyes causes overlap like with the fluorophores FITC and PE. 2) For sensitivity reasons,
More informationBD FACSVerse. Simply brilliant
BD FACSVerse Simply brilliant BD FACSVerse: Simply Brilliant The BD FACSVerse flow cytometer was engineered from the ground up to offer remarkable performance, flexibility, and ease of operation for research
More informationFlow Cytometry. What is Flow Cytometry? What Can a Flow Cytometer Tell Us About a Cell? Particle Size. Flow = Fluid Cyto = Cell Metry = Measurement
What is Flow Cytometry? Flow Cytometry Basic Principle and Applications BD Biosciences Daisy Kuo (daisy_kuo@bd.com) Flow = Fluid Cyto = Cell Metry = Measurement A variety of measurements are made on cells,
More informationAfter completing this module, you will be able to: Describe the BD FACScan system and how it works.
BD FACScan System After completing this module, you will be able to: Describe the BD FACScan system and how it works. Perform instrument startup and shutdown procedures. Describe instrument maintenance
More informationInstructions for Use. CyAn ADP. High-speed Analyzer. Summit 4.3. 0000050G June 2008. Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835
Instructions for Use CyAn ADP High-speed Analyzer Summit 4.3 0000050G June 2008 Beckman Coulter, Inc. 4300 N. Harbor Blvd. Fullerton, CA 92835 Overview Summit software is a Windows based application that
More informationFACSCalibur System User s Guide
11-10823-02 Rev. A FACSCalibur System User s Guide 02-61760-02 August, 1996 Becton Dickinson Immunocytometry Systems 2350 Qume Drive San Jose, CA 95131-1807 Ordering Information (800) 223-8226 Customer
More informationImmunophenotyping Flow Cytometry Tutorial. Contents. Experimental Requirements...1. Data Storage...2. Voltage Adjustments...3. Compensation...
Immunophenotyping Flow Cytometry Tutorial Contents Experimental Requirements...1 Data Storage...2 Voltage Adjustments...3 Compensation...5 Experimental Requirements For immunophenotyping with FITC and
More informationBD CellQuest Pro Software Analysis Tutorial
BD CellQuest Pro Analysis Tutorial This tutorial guides you through an analysis example using BD CellQuest Pro software. If you are already familiar with BD CellQuest Pro software on Mac OS 9, refer to
More informationStandard Operating Procedure
1.0 Purpose: 1.1 The characterisation of of main leukocyte subsets in peripheral blood cells from mice by flow cytometry. Reliable values of frequencies of leukocyte clusters are very much dependent on
More informationSpherotech, Inc. 27845 Irma Lee Circle, Unit 101, Lake Forest, Illinois 60045 1
78 Irma Lee Circle, Unit 0, Lake Forest, Illinois 00 SPHERO TM Technical Note STN- Rev B 008 DETERMINING PMT LINEARITY IN FLOW CYTOMETERS USING THE SPHERO TM PMT QUALITY CONTROL EXCEL TEMPLATE Introduction
More informationImmunophenotyping peripheral blood cells
IMMUNOPHENOTYPING Attune Accoustic Focusing Cytometer Immunophenotyping peripheral blood cells A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune
More informationBD LSR II User s Guide
BD LSR II User s Guide bdbiosciences.com Part No. 642221 Rev A June 2007 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax (800) 325-9637 facservice@bd.com Asia Pacific
More informationIntroduction to Flow Cytometry
Introduction to Flow Cytometry presented by: Flow Cytometry y Core Facility Biomedical Instrumentation Center Uniformed Services University Topics Covered in this Lecture What is flow cytometry? Flow cytometer
More informationLet the BD FACSAria II accompany your best performance ever
Let the BD FACSAria II accompany your best performance ever Meet the new BD FACSAria II high-speed, fixed-alignment cuvette cell sorter. The first generation BD FACSAria system brought the complex world
More information123count ebeads Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use.
Page 1 of 1 Catalog Number: 01-1234 Also known as: Absolute cell count beads GPR: General Purpose Reagents. For Laboratory Use. Normal human peripheral blood was stained with Anti- Human CD45 PE (cat.
More informationTechnical Bulletin. Threshold and Analysis of Small Particles on the BD Accuri C6 Flow Cytometer
Threshold and Analysis of Small Particles on the BD Accuri C6 Flow Cytometer Contents 2 Thresholds 2 Setting the Threshold When analyzing small particles, defined as particles smaller than 3.0 µm, on the
More informationCyFlow Space Your flexible flow cytometer
Your flexible flow cytometer www.sysmex-partec.com its flexibility gives you the space you need for your work Analysing cells and particles, be it from blood, plasma, tissue, plants, cell cultures or other
More informationPreparing BD Systems for Data Migration. Summary of the Upgrade Process on page 6. Copying User Data Files onto an Upgraded System on page 7
Windows 7 Upgrade Instructions 8/2014 Preparing BD Systems for Data Migration Contents Overview Backing Up Your Data on page 2 Summary of the Upgrade Process on page 6 Copying User Data Files onto an Upgraded
More informationThree Rules for Compensation Controls
From FlowJo s Daily Dongle Three Rules for Compensation Controls First and foremost, there must be a single-stained control for every parameter in the experiment! In addition, there are three rules for
More informationTROUBLESHOOTING FLOW CHART
TROUBLESHOOTING FLOW CHART Revised and updated on January 16, 2003, by Tony Leger and Automation Lab Technology. FACScan(FC)\FACSort(FST) FACScalibur (FCB) Fluidics/Pneumatics troubleshooting guide DCM
More informationBD LSRFortessa Cell Analyzer User s Guide
BD LSRFortessa Cell Analyzer User s Guide For Research Use Only bdbiosciences.com 23-11093-00 Rev. A 3/2010 Becton, Dickinson and Company BD Biosciences San Jose, CA 95131 Tel 877.232.8995 Fax 800.325.9637
More informationCell Viability Measurement
Cell Viability Measurement Viability is a measure of the metabolic state of a cell population which is indicative of the potential for growth Most common method: membrane integrity Trypan blue exclusion:
More informationCELL CYCLE BASICS. G0/1 = 1X S Phase G2/M = 2X DYE FLUORESCENCE
CELL CYCLE BASICS Analysis of a population of cells replication state can be achieved by fluorescence labeling of the nuclei of cells in suspension and then analyzing the fluorescence properties of each
More informationAttune Acoustic Focusing Cytometer
USER GUIDE Attune Acoustic Focusing Cytometer USER GUIDE Publication Part Number 4453328 Rev. F Revision Date June 2012 Copyright 2012, Life Technologies Corporation. All rights reserved. For Research
More informationSupplementary Material. Free-radical production after post-thaw incubation of ram spermatozoa is related to decreased in vivo fertility
10.1071/RD14043_AC CSIRO 2015 Supplementary Material: Reproduction, Fertility and Development, 2015, 27(8), 1187 1196. Supplementary Material Free-radical production after post-thaw incubation of ram spermatozoa
More informationNavios Quick Reference
Navios Quick Reference Purpose: The purpose of this Quick Reference is to provide a simple step by step outline of the information needed to perform various tasks on the system. We begin with basic tasks
More informationBD FACSAria Flow Cytometer. The first high-speed sorter with a fixed-alignment cuvette flow cell from BD Biosciences
BD FACSAria Flow Cytometer The first high-speed sorter with a fixed-alignment cuvette flow cell from BD Biosciences Header From Pioneers to Revolutionaries in Flow Cytometry Instrumentation BD Biosciences
More informationApplication Information Bulletin: Set-Up of the CytoFLEX Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement
Application Information Bulletin: Set-Up of the CytoFLEX Set-Up of the CytoFLEX* for Extracellular Vesicle Measurement Andreas Spittler, MD, Associate Professor for Pathophysiology, Medical University
More informationBD FACSAria Flow Cytometer. BD Biosciences Clontech Discovery Labware Immunocytometry Systems Pharmingen
BD FACSAria Flow Cytometer The first high-speed sorter with a fixed-alignment cuvette flow cell from BD Biosciences BD Biosciences Clontech Discovery Labware Immunocytometry Systems Pharmingen From Pioneers
More informationThe NIAID Flow Cytometry Advisory Committee; the Guidelines Subcommittee
January, 1999 From: To: Subject: The NIAID Flow Cytometry Advisory Committee; the Guidelines Subcommittee NIAID DAIDS Flow Cytometry Laboratories Comparison study information for labs wishing to switch
More informationAPPLICATION INFORMATION
DRAFT: Rev. D A-2045A APPLICATION INFORMATION Flow Cytometry 3-COLOR COMPENSATION Raquel Cabana,* Mark Cheetham, Jay Enten, Yong Song, Michael Thomas,* and Brendan S. Yee Beckman Coulter, Inc., Miami FL
More informationMEASURABLE PARAMETERS: Flow cytometers are capable of measuring a variety of cellular characteristics such as:
INTRODUCTION Flow Cytometry involves the use of a beam of laser light projected through a liquid stream that contains cells, or other particles, which when struck by the focused light give out signals
More informationNo-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer
APPLICATION NOTE Attune NxT Flow Cytometer No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer Introduction Standard methods for isolating and detecting leukocytes
More informationStepcount. Product Description: Closed transparent tubes with a metal screen, including a white matrix at the bottom. Cat. Reference: STP-25T
Product Description: Closed transparent tubes with a metal screen, including a white matrix at the bottom Cat. Reference: STP-25T Reagent provided:: 25 Stepcount tubes for 25 test INTENDED USE. Immunostep
More informationCell Sorting. S3e Cell Sorter Quick Guide
Cell Sorting S3e Cell Sorter Quick Guide STARTUP Checking Fluidics STARTUP There are three bulk fluid containers (Figure 1) that may require attention before starting the system. If fluidics need to be
More informationApplication Note 10. Measurement of Cell Recovery. After Sorting with a Catcher-Tube-Based. Cell Sorter. Introduction
Application Note 10 Measurement of Cell Recovery After Sorting with a Catcher-Tube-Based Cell Sorter Introduction In many experiments using sorted cells, it is important to be able to count the number
More informationIntroduction to Flow Cytometry:
Introduction to Flow Cytometry: A Learning Guide Manual Part Number: 11-11032-01 April, 2000 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 1-800-448-2347 Introduction to Flow Cytometry: A Learning
More informationINSIDE THE BLACK BOX
FLOW CYTOMETRY ESSENTIALS INSIDE THE BLACK BOX Alice L. Givan Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School HOW NOT TO BE A FLOW CYTOMETRIST Drawing by Ben
More informationBD FACSDiva Software Reference Manual
BD FACSDiva Software Reference Manual For Version 8.0 IVD For In Vitro Diagnostic Use bdbiosciences.com 23-14523-00 4/2013 Becton, Dickinson and Company BD Biosciences 2350 Qume Dr. San Jose, CA 95131
More informationApplication Note. Cell Cycle and DNA Content Analysis Using the BD Cycletest Assay on the BD FACSVerse System. Summary
Cell Cycle and DNA Content Analysis Using the BD Cycletest Assay on the BD FACSVerse System Yibing Wang, Catherine McIntyre, and Dev Mittar BD Biosciences, San Jose Contents 1 Summary 2 Introduction 3
More informationBelow is a list of things you should be aware of before you schedule your sort.
Sorting at the Flow Cytometry Facility At the present time, the assistance of a trained cell sorter operator is needed for all sorting applications. As such, if you are planning a first time sort you will
More informationBD FACSCanto Flow Cytometer Reference Manual
BD FACSCanto Flow Cytometer Reference Manual For In Vitro Diagnostic Use http://www.bdbiosciences.com/ Part No. 338617 Rev. A September 2004 BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel
More informationBD StemFlow Human Pluripotent Stem Cell Sorting and Analysis Kit
BD StemFlow Human Pluripotent Stem Cell Sorting and Analysis Kit Instruction Manual Catalog No. 560461 ii Human Pluripotent Stem Cell Sorting and Analysis Kit 2009, Becton, Dickinson and Company. All rights
More informationflow cytometry reinvented
FLOW CYTOMETRY flow cytometry reinvented The Attune Acoustic Focusing Cytometer Precision and at all speeds for rare events and precious samples The Attune Acoustic Focusing Cytometer Precise high collection
More informationEasy to use. Compact. Flexible. Sorting Chip
SH800Z Cell Sorter The SH800Z is a compact, affordable, and easy to use cell sorter developed using the state- of-the art technologies from Sony. An array of automated mechanisms makes it an ideal sorter
More informationApplication Note. Multiparametric Immunophenotyping of Human Hematopoietic Stem Cells and Progenitor Cells by Flow Cytometry.
January 212 Multiparametric Immunophenotyping of Human Hematopoietic Stem Cells and Progenitor Cells by Flow Cytometry Xiaoyang (Alice) Wang, Janelle Shook, Mark Edinger, Noel Warner, Charlene Bush-Donovan
More informationNovoCyte Flow Cytometer Operator s Guide
NovoCyte Flow Cytometer Operator s Guide Table of Content Prologue 5 I. Revision History...5 II. Contact Information...6 III. Trademark...6 IV. Intended Use...6 V. Using this Guide...6 VI. Conventions...7
More informationFlow Cytometry for Everyone Else Susan McQuiston, J.D., MLS(ASCP), C.Cy.
Flow Cytometry for Everyone Else Susan McQuiston, J.D., MLS(ASCP), C.Cy. At the end of this session, the participant will be able to: 1. Describe the components of a flow cytometer 2. Describe the gating
More informationIslet Viability Assessment by Single Cell Flow Cytometry
Islet Viability Assessment by Single Cell Flow Cytometry Page 1 of 8 Purpose: To comprehensively assess the viability of the islet cell preparation prior to transplantation. Tissue Samples: A sample containing
More informationTechnical Bulletin. An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers BD Biosciences, San Jose, CA Contents 2 Introduction to compensation 5 Tips for setting up compensation 10 Tools for easy,
More informationBD FACSCalibur Instructions For Use
BD FACSCalibur Instructions For Use IVD For In Vitro Diagnostic Use bdbiosciences.com Part No. 643271 Rev. A November 2007 BD Biosciences San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax (800) 325-9637
More informationAttune NxT Acoustic Focusing Cytometer
USER GUIDE Attune NxT Acoustic Focusing Cytometer Catalog Number A24858, A24859, A24860, A24861, A24862, A24863, A24864 Publication Number 100024235 Revision B.0 For Research Use Only. Not for use in diagnostic
More informationHoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-806)
Hoechst 33342 HSC Staining and Stem Cell Purification Protocol (see Goodell, M., et al. (1996) J Exp Med 183, 1797-86) The Hoechst purification was established for murine hematopoietic stem cells (HSC)
More informationCyTOF2 Sample Acquisition: Super Sampler* (CyTOF software version 6.0.626)
CyTOF2 Sample Acquisition: Super Sampler* (CyTOF software version 6.0.626) *Amended from Sampler Manual created by Zach Bjornson Because CyTOF computer is on a private network, you will be bombarded with
More informationBD FACSCalibur. The flow cytometer for your routine cell analysis needs
BD FACSCalibur The flow cytometer for your routine cell analysis needs A system with a rich application basis and a modular approach that continues to meet evolving needs of cell analysis worldwide. The
More informationDOC026.53.80098. PharmSpec 3. Software Manual. 10/2013, Edition 3
DOC026.53.80098 PharmSpec 3 Software Manual 10/2013, Edition 3 Table of Contents Section 1 PharmSpec 3 description...3 1.1 What is new in PharmSpec 3?...3 1.2 Software components...3 Section 2 Installation...5
More informationCELL CYCLE BASICS. G0/1 = 1X S Phase G2/M = 2X DYE FLUORESCENCE
CELL CYCLE BASICS Analysis of a population of cells replication state can be achieved by fluorescence labeling of the nuclei of cells in suspension and then analyzing the fluorescence properties of each
More informationSA-9600 Surface Area Software Manual
SA-9600 Surface Area Software Manual Version 4.0 Introduction The operation and data Presentation of the SA-9600 Surface Area analyzer is performed using a Microsoft Windows based software package. The
More information