Leica Stereo Explorer. Visualizing and Analyzing Microstructures in 3D. Living up to Life

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1 Leica Stereo Explorer Visualizing and Analyzing Microstructures in 3D Living up to Life

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3 No matter how big the eye, two eyes are better than one (Sudanese proverb) Three-dimensional photography Nature shows us how it works One eye is enough to see. It takes two, however, to visualize the world spatially. The slight convergence of the optic axes generates two slightly differing images, which our brains then merge into a three-dimensional impression of reality. The world thus appears in relief, and we can immediately recognize whether an object is near or far and whether a surface is flat or rugged. Spatial vision helps us everyday yet it becomes indispensable when observing specimens under a stereomicroscope. Today we are capable of expanding this stereo visual effect to photography: Leica Microsystems offers a one-of-a-kind, modular 3D system for the Leica M165 C stereomicroscope that makes it possible to examine 3D images on a computer.

4 Example Anaglyph image of a metal surface. Example Anaglyph image of a fracture surface.

5 Anaglyph image of a metal surface with visible reference plane. Left Eye Right Eye To create anaglyph illustrations, two images of an object are taken from slightly different viewing angles and combined afterwards. The small deviation in the viewing angle is sufficient enought so that our brain can combine them into a spatial 3D image. The same principle is used with the Leica IC 3D camera, which captures two images offset slightly with a different viewing angle. These images are combined by the Leica Stereo Explorer software into a single 3D image. Would you like to learn more about the Leica IC 3D and the Leica Stereo Explorer software? Then visit us at

6 The Stereo Explorer software application generates a stereopair from a single specimen. The left and right image channels show slightly different contours, allowing height to be computed. Differences in height are made visible by an optional, height-specific false-color representation. The digital surface model of a specimen can be rotated in any direction and zoomed. The module Profile analysis illustrates the height profile across any (red) line. Within this profile, numerous measurements can be taken in x, y and z. The reference plane is used for all displayed height data. It can be configured as desired, or recalculated based on user-defined points. Using the auxiliary module Volume, the Stereo Explorer precisely calculates the volumes above and below the reference plane.

7 The fracture surface of a metal bolt is well suited for 3D analysis using the Stereo Explorer software. Examination of a written sample in stereo image representation. Here is a signature written with a blue ball-point pen. This surface model, too, can be encoded for height using false colors and rotated in any way in space. With Stereo Explorer, you can immediately determine the pressure used when the signature was written, and which lines have crossed. The reference plane can be defined as desired and rotated around all axes. It determines the zero point for all subsequent 3D measurements. The Stereo Explorer can also be used to analyze complex specimens without contact and to examine their shapes.

8 The perfect pair for stereo photography As if within grasp: Relief photographs result when the most modern hardware and software come together. The Leica IC 3D digital camera becomes the eyes of the stereomicroscope, while the Stereo Explorer takes over the brain s role: it computes the 3D data record from the stereopairs. Together, they generate a spatial image of the specimen in brilliant colors and high resolution. They apply the newest algorithms of digital image processing so that the camera and the objective can show off their proverbial Leica qualities. The full program at the touch of a button With a modular design, the Stereo Explorer can be expanded to include new functions at any time. The Autofocus module, for example, can be utilized to control the motorized focus, while the Profile module graphically displays the profile of a surface. Stereo Explorer offers the precise functions that you need for your work. Auto Calibration mode The calibration tool (part no ) applies softwarebased corrections to manufacturing tolerances in the optics or mechanical system. This simply requires that the calibration tool is set to a specific zoom position using the integrated calibration assistant everything else is handled by the system automatically. Leave a lasting impression From the data attained, the Leica 3D Viewer models the Digital Surface Model (DSM). This can be used, for example, to draw the original 2D image as a texture onto the DSM. And to illustrate the specimen s vertical scale, a height-encoded false-color representation is used. Naturally, the surface model can be rotated in any direction and zoomed. Once you are completely satisfied with the results, save the view in a popular graphics format, such as TIFF or JPEG. Now, your presentations will look better than ever! Leica Stereo Explorer A System Overview 1 A stereopair is generated by an integrated camera. 2 The Leica IC 3D is suited for the Leica M165 C and MZ16 A stereomicroscopes. 3 The motorized focus is controlled by the Stereo Explorer and ensures that a uniform depth effect is attained. 4 If no PC is connected, the Leica SmartTouch can also be used as the controller. 5 Possible to view sample while using the Leica IC 3D. 6 High-quality Leica objectives yield crystal clear images. 7 The Leica LED5000 RL ring light provides the optimum illumination for stereo photography. 8 Both the camera and the software can be used with a wide variety of Leica accessories. Important details about the Leica IC 3D digital camera megapixel CCD with Bayer Array RGB filter generate outstanding color pictures. Fast data transfer through a unique FireWire connection. Connecting the camera to any stereomicroscope of the Leica M series is quick and easy no adjustments necessary. Important details about the Leica Stereo Explorer software The live image function makes focusing and positioning fast. Stereoscopic live viewing of specimens with anaglyph technology. The intuitive and easy-to-use interface reduces time need to learn how to use the software. The ergonomic integration of motorized stereomicroscopes makes repetitive tasks easier. Software modules for profile, roughness, surface and volume analysis can be added at any time. Stereo Explorer supports the Leica M165 C and MZ16 A stereomicroscopes.

9 Leica M165 C precise, efficient, compact

10 Leica IC 3D camera Technical data, performance characteristics Camera type CCD stereo camera with control software Compatibility Compatible with all stereomicroscopes of the M series Energy consumption ~6 W Sensor type RGB Bayer Mosaic Shutter control Electronic global shutter/interlaced scan mode Resolution megapixels ( ) Max. interpolated resolution megapixels ( ) Color depth 36-bit Exposure time 230 µs 30 s Live image On computer screen for all formats Cooling Passive heat dissipation via metal housing Image formats & photography modes Images per second: fast / HQ Resolution Mono Stereo Full image, color or monochrome / / binning, color or monochrome / 5 5 / binning, color or monochrome / / binning, color or monochrome / / 5 Progressive sub-sample / NA 16.5 / NA Progressive R or G B monochrome / 5 5 / 2.5 Modes Formats in fast (20 MHz) or high-quality (10 MHz) modes as specified above, triggered or free running Computer requirements Hardware Operating system Required software Interface Pentium 4 with 2 GHz, 512 MB, 24-bit graphics card, , CD-ROM drive, onboard 1394a FireWire OHCI or available PCI slot for FireWire PCI card Windows 2000, Windows XP Leica DFC Twain / Leica Stereo Explorer FireWire 6-pin (IEEE1394a) Leica IC 3D: Technical data and operating environment Housing Die-cast aluminum Dimensions mm (W H D) Weight 550 g Permissible temperature range +10 to +35 C Relative humidity 10% to 80%, non-condensing

11 Article descriptions Eyepieces and tubes Wide-field eyepieces for eyeglass wearers, 10 /23, distortion-free, dioptric correction, with eyecups (replaceable) Binocular tube ErgoTube Straight tube Binocular Ergo- Tube Inclined binocular tube, 45 viewing angle Binocular ErgoTube with 45 viewing angle and long tubes Binocular straight tube with 90 viewing angle Binocular ErgoTube with variable viewing angle Camera Leica IC 3D camera kit (includes 2 m 6-pin/6-pin FireWire cable, Leica DFC Twain software) IC 3D Zoom systems and accessories Leica M165 C, 16.5:1 zoom system, apochromatic, electronically encoded Focusing drive Motorized focus with 420 mm profile column Motorized focus with 620 mm profile column SmartTouch TM Handwheel Motorized focus short long 0.63 planapochromat M165 C planapochromat Controls SmartTouch, external control unit with integr. touchscreen for status control and control of all settings and functions Manual controller for motor focus Planapochromatic objectives, parfocal Planapochromatic objective 0.63, working distance 67 mm 80 mm Planapochromatic objective 1.0, working distance 61.5 mm 80 mm Planapochromatic objective 1.6, working distance 30.5 mm 80 mm Planapochromatic objective 2, working distance 20.1 mm 80 mm Gliding stage Incident-light base planapochromat planapochromat Illuminators Leica LED5000 RL ring illuminator with 48 LEDs Leica Fluo ring illuminator Adapter ring for Leica Fluo ring illuminator LED5000 RL Base and stage Incident-light base Gliding stage, 120 mm PC and monitor PC " TFT monitor for ShuttlePC Software Leica Stereo Explorer, core Autofocus * Profile * Surface * Volume * Calibration tool * auxiliary module

12 With the user, for the user Leica Microsystems Leica Microsystems operates globally in four divi sions, where we rank with the market leaders. Life Science Division The Leica Microsystems Life Science Division supports the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientific applications puts Leica Microsystems customers at the leading edge of science. Industry Division The Leica Microsystems Industry Division s focus is to support customers pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications. Biosystems Division The Leica Microsystems Biosystems Division brings histopathology labs and researchers the highest-quality, most comprehensive product range. From patient to pathologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems featuring innovative automation and Novocastra reagents, Leica Microsystems creates better patient care through rapid turnaround, diagnostic confidence, and close customer collaboration. Surgical Division The Leica Microsystems Surgical Division s focus is to partner with and support surgeons and their care of patients with the highest-quality, most innovative surgi cal microscope technology today and into the future. The statement by Ernst Leitz in 1907, with the user, for the user, describes the fruitful collaboration with end users and driving force of innovation at Leica Microsystems. We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. Active worldwide Australia: North Ryde Tel Fax Austria: Vienna Tel Fax Belgium: Groot Bijgaarden Tel Fax Canada: Richmond Hill/Ontario Tel Fax Denmark: Herlev Tel Fax France: Rueil-Malmaison Tel Fax Germany: Wetzlar Tel Fax Italy: Milan Tel Fax Japan: Tokyo Tel Fax Korea: Seoul Tel Fax Netherlands: Rijswijk Tel Fax People s Rep. of China: Hong Kong Tel Fax Portugal: Lisbon Tel Fax Singapore Tel Fax Spain: Barcelona Tel Fax Sweden: Kista Tel Fax Switzerland: Heerbrugg Tel Fax United Kingdom: Milton Keynes Tel Fax USA: Bannockburn/lllinois Tel Fax and representatives in more than 100 countries Order nos. of the editions in: English?????? German?????? French?????? Spanish?????? Italian?????? Part-No.???-?????/09/???/???? LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. 10IDI10010en Leica Microsystems (Switzerland) Ltd CH-9435 Heerbrugg, 2009 Printed in Switzerland II.2009 RDV Illustrations, descriptions and technical data are not binding and may be changed without notice.