Leica Microdissection Objectives

Size: px

Start display at page:

Download "Leica Microdissection Objectives"

Earl Ross
7 years ago
Views:

1 Leica Microdissection Objectives resolution

2 Leica Microdissection Objectives Laser microdissection has developed into one of the most important methods of specimen preparation. Specimens for gene expression profiling, proteomics, and various molecular diagnostic applications must consist of a homogenous cell population. For this purpose, selecting cells from tissue sections by means of microdissection has proven itself as a valuable tool. Increasingly, microdissection applications create molecular signatures as the basis for functional studies of clinical images or agent analysis. By using microdissection, a separate analysis of different cells from mixed tissues can provide a comprehensive view into tumor genesis. High quality optics are at the heart of laser microdissection, and image quality must meet the expected high standards of scientists. Also, high transmission in the UV range is required, coupled with excellent imaging of the UV laser through the objective. Leica Microsystems has developed a new set of objectives that optimize these functions and provide the best possible performance for single cell or larger tissue area microdissection. Leica Microsystems new SmartCut Series of Objectives range from 6.3x to 150x. Leica SmartCut objectives enhance all applications of proteomics including the selection of single chromosomes from metaphase spreads. 2

For this purpose, selecting cells from tissue sections by means of microdissection has proven itself as a valuable tool.

3 HCX PL FLUOTAR UVI 6.3x /0.13 Microdissection HCX PL FLUOTAR L 40x/0.60 CORR XT a b Pre-LMD Post-LMD Cap H&E stained left ventricular section shown above, prior to and following laser microdissection. The specimen is then shown in the cap. (a) Microdissection of a blood vessel; (b) Microdissection of a group of cardiac myocytes. The bar represents 100 µm. M r a IPG 3-10 NL x b IPG 3-10 NL Two-dimensional gel separations from H&E stained sections either (a) scraped or (b) laser microdissected. Protein spots were visualized by silver staining. 50 µm Section of muscle (human) following dual histochemistry for cytochrome c oxidase (COX) and succinate dehydrogenase (SDH), both mitochondrial enzymes. The blue muscle fibre is deficient in COX. Used with permission of Dr. G. Borthwick, Institute of Human Genetics, University of Newcastle Used with permission of Drs. A.I. De Souza and M.L. Rose. Images courtesy of Heart Science Centre, Harefield Hospital: De Souza, A.I.; McGregor, E.; Dunn, M. J.; Rose, M. L., Proteomics, 4, (2004). Leica Microsystems UVI 6.3x objective was specifically designed for process applications in proteomics. High UV transmission at low magnification makes this objective a sophisticated tool for dissecting larger tissue areas from frozen or paraffin sections. High quality imaging of the laser focus in the specimen plane allows the user to effortlessly dissect thicker specimens without a significant widening of the dissection line. The clearly illuminated field of view also makes this objective an important tool for generating a good overall view of the cell(s) of interest. Leica Microsystems new 40x objective sets new standards in microdissection. Providing excellent quality and the highest UV transmission, this objective is the standard tool for almost all microdissection applications. Leica Microsystems 40x objective dissects even complex materials with a low dissecting line width. The 40x objective operates with a low effective laser aperture, which allows the laser beam to be precisely positioned in thicker specimens. This also makes this objective well-suited for laser ablation, among other techniques. The objective s correction ring ensures consistently excellent image quality. Order No Order No

5 14.3 b IPG 3-10 NL Two-dimensional gel separations from H&E stained sections either (a) scraped or (b) laser microdissected. Protein spots were visualized by silver staining.

4 HCX PL FLUOTAR L 63x/0.70 CORR XT HCX PL FLUOTAR L 150x/0.90 Microdissection 150x HCX PL Fluotar, Metaphase Chromosomes on PET membrane slides Astrocytes: Immunfluorescence with GFAP primary and Cy3 secondary antibody Before cut During cut RT-PCR analysis of microdissected astrocytes from mouse cortex Cutting line on a 15 µm rat colon paraffin section stained with H&E C 1 2 C 1 2 C 1 2 Cyclophilin GFAP Neurofilament C: Whole brain control 1; 2: Astrocytes, microdissected (n= 10 cells) : no cdna input Small dissections down to a width of one micrometers are required to select cells in the range of 5-20 µm in diameter. Dissecting single cells of this width requires special optics. With Leica Microsystems 63x HCX PL FLUOTAR objective, these requirements are achieved with brilliant image quality. The large working distance of this objective allows easy and safe microdissection when working with groups or individual cells. Leica Microsystems HCX PL FLUOTAR 150x/0.9 Microdissection objective represents a special development. This150x magnification objective with high aperture creates dissecting line widths in the specimen of less than one micrometer. Leica designed the objective as a dry objective, which makes working at high magnification practical and easy. At the same time, this objective features an extremely brilliant image quality at an aperture of 0.9. Whether the tasks involve dissecting single astrocytes or chromosomes, this objective is the perfect solution whenever highest magnifications with extremely small dissecting widths are required. Order No Order No

of microdissected astrocytes from mouse cortex Cutting line on a 15 µm rat colon paraffin section stained with H&E C 1 2 C 1 2 C 1 2 Cyclophilin GFAP Neurofilament C: Whole brain control 1; 2:

5 5

6 Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first-choice provider of innovative solutions to our customers needs for vision, measurement, lithography and analysis of microstructures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. Leica Microsystems an international company with a strong network of customer services Australia: Gladesville Tel Fax Austria: Vienna Tel Fax Canada: Richmond Hill/Ontario Tel Fax Denmark: Herlev Tel Fax France: Rueil-Malmaison Tel Fax Germany: Bensheim Tel Fax Italy: Milan Tel Fax Japan: Tokyo Tel Fax Korea: Seoul Tel Fax Netherlands: Rijswijk Tel Fax People s Rep. of China: Hong Kong Tel Fax Portugal: Lisbon Tel Fax Singapore Tel Fax Spain: Barcelona Tel Fax Sweden: Sollentuna Tel Fax Switzerland: Glattbrugg Tel Fax United Kingdom: Milton Keynes Tel Fax USA: Bannockburn/lllinois Tel Fax and representatives of Leica Microsystems in more than 100 countries. The companies of the Leica Microsystems Group operate internationally in four business segments, where we rank with the market leaders. Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. Specimen Preparation We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers. Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. Copyright Leica Microsystems Wetzlar GmbH Ernst-Leitz-Strasse Wetzlar Germany 2005 Tel. ( ) 29-0 Fax ( ) LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. Order nos. of the editions in: English German Part-No Printed on chlorine-free bleached

Leica DM LS2 and DM LB2. Life Science System Microscopes with New Ergonomic Concept

Leica DM LS2 and DM LB2. Life Science System Microscopes with New Ergonomic Concept Leica DM LS2 and DM LB2 Life Science System Microscopes with New Ergonomic Concept Worldwide Customer Base Two universal specialists The universal system microscopes Leica DM LS2 and DM LB2 can be used