Tackling the data analysis challenge for characterisation of biotherapeutics
|
|
- Nicholas McGee
- 8 years ago
- Views:
Transcription
1 CASSS AT 2015 Berlin March Tackling the data analysis challenge for characterisation of biotherapeutics Carsten P Sönksen, Ph.D., Novo Nordisk
2 Tackling the data analysis challenge 2 Personal background: Carsten P Sönksen Experience: ~19 years with mass spectrometry ~14 years in an industrial setting Responsibility: Senior Research Scientist, responsible for MS-based protein characterisation of biopharmaceuticals Dept.: CMC - analytical support, Novo Nordisk Implemented Genedata Expressionist for vendor independent data processing and analysis
3 Tackling the data analysis challenge 3 Case study: Characterisation of the charged isoforms of stressed IgG4 Stressed IgG4 sample was analysed by icief Preparative isoelectric focusing (Agilent) of fractions for characterisation by MS MS-characterisation by Intact mass analysis LC-MS (Waters) Tryptic peptide map LC-MS/MS (Thermo) Raw data processing, analysis, and reporting Genedata Expressionist for Mass Spectrometry
4 Tackling the data analysis challenge icief electropherograms of stressed sample and isolated preparative fractions Challenge: Several peaks in each fraction pi Load Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3
5 Presentation title 5 Intact LC-MS data: Single sample view vs. overlay Weak patterns become clearer Acidic 3 Main form: +2 G0F, - 2 K, 2 Pyro-glu Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 -F: -146 Da + K: +128 Da +2 K: +256 Da - 2 Pyro-glu: + 34 Da + G: G: Da Mass
6 Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3 Presentation title 6 Quality check of tryptic peptide maps (LC-MS/MS): Box Plot analysis 1: Average signal intensity analysis - check for differences - check for abnormal runs 2: Set Acidic 2 and Basic 3 on the watch list
7 Tackling the data analysis challenge 7 Guided vs. blind analysis: Only a fraction of the peaks are identified (red crosses) Acidic 3 peptide map TIC chromatogram Acidic 3 peptide map TIC 2D heat map
8 Many unidentified high intense peaks still ask for identification ~ 345 charge clusters not identified out of 530! A need for further identification analysis. Mass Charge 4 Mass Charge 3 Tackling the data analysis challenge 8
9 Presentation title 9 Are the detected modification valid? Check modifications in the mass spectral data Cleaned Data Raw Data Dominant form HC aa Dehydrated Form? Deamidated Form Succinimide Form
10 Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3 Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3 Presentation title Date 10 Absence of the C-terminal lysine (K) Peptide with C-terminal lysine on the HC Peptide without C-terminal lysine on the HC
11 Presentation title Date 11 Unbiased Statistic Analysis of Charged Isoforms Contrast analysis: Only the HC C-terminal lysine peptides describe the difference in the preparative fractions with a significance of ~ P<0.05
12 Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3 Acidic 3 Acidic 2 Acidic 1 Major 1 Major 2 Basic 1 Basic 2 Basic 3 Tackling the data analysis challenge Date 12 Distribution of deamidated and succinimide forms Check signal in raw data Deamidated Δ = Da Δ = Da Succinimide
13 Presentation title Date 13 Distribution of N-glycosylation forms G0F G1F G0 No N-glyco
14 Presentation title 14 Conclusion Loss of lysine on the C-terminal of the heavy chain explains the basic isoforms Deamidation and succinimide explain only part of the acidic isoforms Neutral modifications like G0F are fractionated as well G0F, - 2 K, 2 Pyro-glu G1F G Deamidation C-term Lysine Deamidation
15 Presentation title Genedata Expressionist has become our standard platform for biopharmaceutical characterisation Why did we look beyond vendor software So much data, so little time Instruments from 5 vendors -> 5 softwares to learn Automating standardized work and using free time to dig deeper into interesting peaks... Workflow-based system enables: - Reporting of data, results and parameters - Swift analysis of samples in parallel - Fast iterations for reanalysis - Excellent visualisation of data - Confirmation of results in raw data Date 15
16 Tackling the data analysis challenge 16 Data analytical recommendations Overlaying of parallel processed data is a simple powerful approach to verify patterns Box plot analysis is a simple and fast analysis to check peptide load integrity between samples Automation saves time on the routine tasks which can be spent on high intense unidentified peaks Always verify conclusion by looking at both the processed and raw data Sophisticated visualization aids unbiased and complete characterization
17 Tackling the data analysis challenge 17 Thanks to Brian Kristensen, Novo Nordisk Ingelise Fabrin, Novo Nordisk Leif H. Bagger, Novo Nordisk Arnd Brandenburg, Genedata
Error Tolerant Searching of Uninterpreted MS/MS Data
Error Tolerant Searching of Uninterpreted MS/MS Data 1 In any search of a large LC-MS/MS dataset 2 There are always a number of spectra which get poor scores, or even no match at all. 3 Sometimes, this
More informationChoices, choices, choices... Which sequence database? Which modifications? What mass tolerance?
Optimization 1 Choices, choices, choices... Which sequence database? Which modifications? What mass tolerance? Where to begin? 2 Sequence Databases Swiss-prot MSDB, NCBI nr dbest Species specific ORFS
More informationLearning Objectives:
Proteomics Methodology for LC-MS/MS Data Analysis Methodology for LC-MS/MS Data Analysis Peptide mass spectrum data of individual protein obtained from LC-MS/MS has to be analyzed for identification of
More informationAB SCIEX TOF/TOF 4800 PLUS SYSTEM. Cost effective flexibility for your core needs
AB SCIEX TOF/TOF 4800 PLUS SYSTEM Cost effective flexibility for your core needs AB SCIEX TOF/TOF 4800 PLUS SYSTEM It s just what you expect from the industry leader. The AB SCIEX 4800 Plus MALDI TOF/TOF
More informationIn-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates
In-Depth Qualitative Analysis of Complex Proteomic Samples Using High Quality MS/MS at Fast Acquisition Rates Using the Explore Workflow on the AB SCIEX TripleTOF 5600 System A major challenge in proteomics
More informationThermo Scientific PepFinder Software A New Paradigm for Peptide Mapping
Thermo Scientific PepFinder Software A New Paradigm for Peptide Mapping For Conclusive Characterization of Biologics Deep Protein Characterization Is Crucial Pharmaceuticals have historically been small
More informationA Streamlined Workflow for Untargeted Metabolomics
A Streamlined Workflow for Untargeted Metabolomics Employing XCMS plus, a Simultaneous Data Processing and Metabolite Identification Software Package for Rapid Untargeted Metabolite Screening Baljit K.
More informationProteinScape. Innovation with Integrity. Proteomics Data Analysis & Management. Mass Spectrometry
ProteinScape Proteomics Data Analysis & Management Innovation with Integrity Mass Spectrometry ProteinScape a Virtual Environment for Successful Proteomics To overcome the growing complexity of proteomics
More informationLaboration 1. Identifiering av proteiner med Mass Spektrometri. Klinisk Kemisk Diagnostik
Laboration 1 Identifiering av proteiner med Mass Spektrometri Klinisk Kemisk Diagnostik Sven Kjellström 2014 kjellstrom.sven@gmail.com 0702-935060 Laboration 1 Klinisk Kemisk Diagnostik Identifiering av
More informationTutorial for Proteomics Data Submission. Katalin F. Medzihradszky Robert J. Chalkley UCSF
Tutorial for Proteomics Data Submission Katalin F. Medzihradszky Robert J. Chalkley UCSF Why Have Guidelines? Large-scale proteomics studies create huge amounts of data. It is impossible/impractical to
More informationA Navigation through the Tracefinder Software Structure and Workflow Options. Frans Schoutsen Pesticide Symposium Prague 27 April 2015
A Navigation through the Tracefinder Software Structure and Workflow Options Frans Schoutsen Pesticide Symposium Prague 27 April 2015 Kings day in The Netherlands 1 Index Introduction Acquisition, Method
More informationSTANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD 175 STANFORD, CA 94305-5080
Training on the ZQ Open access MS Questions? Contact Dr. Allis Chien allis@stanford.edu 650-723-0710 0710 STANFORD UNIVERSITY MASS SPECTROMETRY STANFORD UNIVERSITY MASS SPECTROMETRY 333 CAMPUS DR., MUDD
More informationEffects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests
Effects of Intelligent Data Acquisition and Fast Laser Speed on Analysis of Complex Protein Digests AB SCIEX TOF/TOF 5800 System with DynamicExit Algorithm and ProteinPilot Software for Robust Protein
More informationRetrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem
Retrospective Analysis of a Host Cell Protein Perfect Storm: Identifying Immunogenic Proteins and Fixing the Problem Kevin Van Cott, Associate Professor Dept. of Chemical and Biomolecular Engineering Nebraska
More informationCliquid ChemoView 3.0 Software Simple automated analysis, from sample to report
PRODUCT BULLETIN Cliquid ChemoView 3.0 Software for Routine Screening and Quantitation Cliquid ChemoView 3.0 Software Simple automated analysis, from sample to report KEY FEATURES Secure user login that
More informationIndustry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development
Industry Perspective: Advantages of Open Access and Walkup LC/ MS Supporting Protein Drug Discovery and Development Dawn Stickle, Agilent Technologies Originally presented by Eric Fang, Novartis Overview
More informationBiopharmaceutical Glycosylation Analysis
Biopharmaceutical Glycosylation Analysis Glycosylation Analysis: Product Offering Molecular model of erythropoietin with complex N-linked glycans. Courtesy of M.R Wormald and R.A Dwek, Oxford Glycobioloy
More informationQuantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry
Quantification of Multiple Therapeutic mabs in Serum Using microlc-esi-q-tof Mass Spectrometry Paula Ladwig, David Barnidge, Mindy Kohlhagen, John Mills, Maria Willrich, Melissa R. Snyder and David Murray
More informationMultiQuant Software 2.0 for Targeted Protein / Peptide Quantification
MultiQuant Software 2.0 for Targeted Protein / Peptide Quantification Gold Standard for Quantitative Data Processing Because of the sensitivity, selectivity, speed and throughput at which MRM assays can
More informationNUVISAN Pharma Services
NUVISAN Pharma Services CESI MS Now available! 1st CRO in Europe! At the highest levels of quality. LABORATORY SERVICES Equipment update STATE OF THE ART AT NUVISAN CESI MS Now available! 1st CRO in Europe!
More informationHenry Shion, Robert Birdsall, Steve Cubbedge, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS INTRODUCTION WATERS SOLUTIONS
Development of Integrated Informatics Workflows for the Automated Assessment of Comparability for Antibody Drug Conjugates (ADCs) Using LC-UV and LC-UV/MS Henry Shion, Robert Birdsall, Steve Cubbedge,
More informationIntroduction. What Can an Offline Desktop Processing Tool Provide for a Chemist?
Increasing Chemist Productivity in an Open-Access Environment Ryan Sasaki, Graham A. McGibbon, Steve Hayward Featuring ACD/Spectrus Processor and Aldrich Library for ACD/Labs Advanced Chemistry Development,
More informationAlignment and Preprocessing for Data Analysis
Alignment and Preprocessing for Data Analysis Preprocessing tools for chromatography Basics of alignment GC FID (D) data and issues PCA F Ratios GC MS (D) data and issues PCA F Ratios PARAFAC Piecewise
More informationMetabolomics Software Tools. Xiuxia Du, Paul Benton, Stephen Barnes
Metabolomics Software Tools Xiuxia Du, Paul Benton, Stephen Barnes Outline 2 Introduction Software Tools for LC-MS metabolomics Software Tools for GC-MS metabolomics Software Tools for Statistical Analysis
More informationMaster course KEMM03 Principles of Mass Spectrometric Protein Characterization. Exam
Exam Master course KEMM03 Principles of Mass Spectrometric Protein Characterization 2010-10-29 kl 08.15-13.00 Use a new paper for answering each question! Write your name on each paper! Aids: Mini calculator,
More informationLa Protéomique : Etat de l art et perspectives
La Protéomique : Etat de l art et perspectives Odile Schiltz Institut de Pharmacologie et de Biologie Structurale CNRS, Université de Toulouse, Odile.Schiltz@ipbs.fr Protéomique et Spectrométrie de Masse
More informationIntroduction to Proteomics 1.0
Introduction to Proteomics 1.0 CMSP Workshop Tim Griffin Associate Professor, BMBB Faculty Director, CMSP Objectives Why are we here? For participants: Learn basics of MS-based proteomics Learn what s
More informationAdvantages of the LTQ Orbitrap for Protein Identification in Complex Digests
Application Note: 386 Advantages of the LTQ Orbitrap for Protein Identification in Complex Digests Rosa Viner, Terry Zhang, Scott Peterman, and Vlad Zabrouskov, Thermo Fisher Scientific, San Jose, CA,
More informationApplication Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities
Application Note # LCMS-66 Straightforward N-glycopeptide analysis combining fast ion trap data acquisition with new ProteinScape functionalities Introduction Glycosylation is one of the most common and
More informationDefinition of the Measurand: CRP
A Reference Measurement System for C-reactive Protein David M. Bunk, Ph.D. Chemical Science and Technology Laboratory National Institute of Standards and Technology Definition of the Measurand: Human C-reactive
More informationAccurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances
Accurate Mass Screening Workflows for the Analysis of Novel Psychoactive Substances TripleTOF 5600 + LC/MS/MS System with MasterView Software Adrian M. Taylor AB Sciex Concord, Ontario (Canada) Overview
More informationAdvances in Protein Characterization
Rapid Assessment of Molecular Similarity between a Candidate Biosimilar and an Innovator Monoclonal Antibody Using Complementary LC MS Methods Intact protein LC MS detected a mass variance of 62 Da and
More informationWorkshop IIc. Manual interpretation of MS/MS spectra. Ebbing de Jong. Center for Mass Spectrometry and Proteomics Phone (612)625-2280 (612)625-2279
Workshop IIc Manual interpretation of MS/MS spectra Ebbing de Jong Why MS/MS spectra? The information contained in an MS spectrum (m/z, isotope spacing and therefore z ) is not enough to tell us the amino
More informationApplication Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software
Application Note # LCMS-62 Walk-Up Ion Trap Mass Spectrometer System in a Multi-User Environment Using Compass OpenAccess Software Abstract Presented here is a case study of a walk-up liquid chromatography
More informationThermo Scientific SIEVE Software for Differential Expression Analysis
m a s s s p e c t r o m e t r y Thermo Scientific SIEVE Software for Differential Expression Analysis Automated, label-free, semi-quantitative analysis of proteins, peptides, and metabolites based on comparisons
More informationDMPK: Experimentation & Data
DMPK: Experimentation & Data Interpretation Mingshe Zhu, Mike S. Lee, Naidong Weng, and Mark Hayward Prerequisite: Entry-level scientists with hands on experience in LC/MS as well as advanced students
More informationProSightPC 3.0 Quick Start Guide
ProSightPC 3.0 Quick Start Guide The Thermo ProSightPC 3.0 application is the only proteomics software suite that effectively supports high-mass-accuracy MS/MS experiments performed on LTQ FT and LTQ Orbitrap
More informationSearching Nucleotide Databases
Searching Nucleotide Databases 1 When we search a nucleic acid databases, Mascot always performs a 6 frame translation on the fly. That is, 3 reading frames from the forward strand and 3 reading frames
More information1 Genzyme Corp., Framingham, MA, 2 Positive Probability Ltd, Isleham, U.K.
Overview Fast and Quantitative Analysis of Data for Investigating the Heterogeneity of Intact Glycoproteins by ESI-MS Kate Zhang 1, Robert Alecio 2, Stuart Ray 2, John Thomas 1 and Tony Ferrige 2. 1 Genzyme
More informationOplAnalyzer: A Toolbox for MALDI-TOF Mass Spectrometry Data Analysis
OplAnalyzer: A Toolbox for MALDI-TOF Mass Spectrometry Data Analysis Thang V. Pham and Connie R. Jimenez OncoProteomics Laboratory, Cancer Center Amsterdam, VU University Medical Center De Boelelaan 1117,
More information3. Career Tools Podcasts
Workshop minutes: Title: Young Mass spectrometrists Workshop Date: June 1 st, 2015 Host: Olga Friese and Kristin Wildsmith Panelist: Industry: Lisa Marzilli, Daniel Spellman Academia: Leslie Hicks Attendees:
More informationFast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3
Fast and Automatic Mapping of Disulfide Bonds in a Monoclonal Antibody using SYNAPT G2 HDMS and BiopharmaLynx 1.3 Hongwei Xie and Weibin Chen Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B
More informationMass Spectrometry Signal Calibration for Protein Quantitation
Cambridge Isotope Laboratories, Inc. www.isotope.com Proteomics Mass Spectrometry Signal Calibration for Protein Quantitation Michael J. MacCoss, PhD Associate Professor of Genome Sciences University of
More informationASMS Regulated Bioanalysis Interest Group (RBIG) Workshop. Antibody-Drug Conjugates (ADC) A Complex Problem in Regulated Bioanalysis.
ASMS Regulated Bioanalysis Interest Group (RBIG) Workshop Antibody-Drug Conjugates (ADC) A Complex Problem in Regulated Bioanalysis June 17, 2014 Presiding: Dr. Keyang Xu (Genentech) and Dr. Fabio Garofolo
More informationMarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis
MarkerView Software 1.2.1 for Metabolomic and Biomarker Profiling Analysis Overview MarkerView software is a novel program designed for metabolomics applications and biomarker profiling workflows 1. Using
More informationMonoclonal Antibody Fragment Separation and Characterization Using Size Exclusion Chromatography Coupled with Mass Spectrometry
Monoclonal ntibody Fragment Separation and haracterization Using Size Exclusion hromatography oupled with Mass Spectrometry uthors Haiying hen Katherine McLaughlin Sepax Technologies, Inc. 5 Innovation
More informationSession 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists
Program Overview Session 1. Course Presentation: Mass spectrometry-based proteomics for molecular and cellular biologists Session 2. Principles of Mass Spectrometry Session 3. Mass spectrometry based proteomics
More informationProteomic Analysis using Accurate Mass Tags. Gordon Anderson PNNL January 4-5, 2005
Proteomic Analysis using Accurate Mass Tags Gordon Anderson PNNL January 4-5, 2005 Outline Accurate Mass and Time Tag (AMT) based proteomics Instrumentation Data analysis Data management Challenges 2 Approach
More informationMassHunter for Agilent GC/MS & GC/MS/MS
MassHunter for Agilent GC/MS & GC/MS/MS Next Generation Data Analysis Software Presented by : Terry Harper GC/MS Product Specialist 1 Outline of Topics Topic 1: Introduction to MassHunter Topic 2: Data
More informationProtein Prospector and Ways of Calculating Expectation Values
Protein Prospector and Ways of Calculating Expectation Values 1/16 Aenoch J. Lynn; Robert J. Chalkley; Peter R. Baker; Mark R. Segal; and Alma L. Burlingame University of California, San Francisco, San
More informationMass Frontier 7.0 Quick Start Guide
Mass Frontier 7.0 Quick Start Guide The topics in this guide briefly step you through key features of the Mass Frontier application. Editing a Structure Working with Spectral Trees Building a Library Predicting
More informationApplication Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008
Bruker Daltonics Application Note # MT-90 MALDI-TDS: A Coherent MALDI Top-Down-Sequencing Approach Applied to the ABRF-Protein Research Group Study 2008 In the ABRF-PRG study 2008 [*] the ability to characterize
More informationPesticide residue analysis: New Trends. Michal Godula, Ph.D. EU Marketing Manager Food Safety & Environmental Thermo Fisher Scientific
Pesticide residue analysis: New Trends Michal Godula, Ph.D. EU Marketing Manager Food Safety & Environmental Thermo Fisher Scientific Why pesticide analysis? Extensively used in agriculture to control
More informationHigh Resolution Epitope Mapping of Human Autoimmune Sera against Antigens CENPA and KDM6B. PEPperPRINT GmbH Heidelberg, 06/2014
High Resolution Epitope Mapping of Human Autoimmune Sera against Antigens CENPA and KDM6B PEPperPRINT GmbH Heidelberg, 06/2014 Introduction This report describes the epitope mapping of autoimmune sera
More informationMASCOT Search Results Interpretation
The Mascot protein identification program (Matrix Science, Ltd.) uses statistical methods to assess the validity of a match. MS/MS data is not ideal. That is, there are unassignable peaks (noise) and usually
More informationTutorial 9: SWATH data analysis in Skyline
Tutorial 9: SWATH data analysis in Skyline In this tutorial we will learn how to perform targeted post-acquisition analysis for protein identification and quantitation using a data-independent dataset
More informationProteinPilot Report for ProteinPilot Software
ProteinPilot Report for ProteinPilot Software Detailed Analysis of Protein Identification / Quantitation Results Automatically Sean L Seymour, Christie Hunter SCIEX, USA Pow erful mass spectrometers like
More informationQuantitative mass spectrometry in proteomics: a critical review
Anal Bioanal Chem (2007) 389:1017 1031 DOI 10.1007/s00216-007-1486-6 REVIEW Quantitative mass spectrometry in proteomics: a critical review Marcus Bantscheff & Markus Schirle & Gavain Sweetman & Jens Rick
More informationGlobal and Discovery Proteomics Lecture Agenda
Global and Discovery Proteomics Christine A. Jelinek, Ph.D. Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences Middle Atlantic Mass Spectrometry Laboratory Global
More informationUSP's Therapeutic Peptides Expert Panel discusses manufacturing processes and impurity control for synthetic peptide APIs.
Control Strategies for Synthetic Therapeutic Peptide APIs Part III: Manufacturing Process Considerations By Brian Gregg,Aleksander Swietlow,Anita Y. Szajek,Harold Rode,Michael Verlander,Ivo Eggen USP's
More informationApplication Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow
Application Note # LCMS-92 Interlaboratory Tests Demonstrate the Robustness and Transferability of the Toxtyper Workflow Abstract There is high demand in clinical research and forensic toxicology for comprehensive,
More informationHigh-Throughput 3-D Chromatography Through Ion Exchange SPE
High-Throughput 3-D Chromatography Through Ion Exchange SPE Application Note 205 Luke Roenneburg and Alan Hamstra (Gilson, Inc.) Introduction 2-dimensional (2-D) separation is the separation of a sample
More informationIntroduction to Bioprocessing
Introduction to Bioprocessing Cambridge Healthtech Institute Peptalk Palm Springs, CA Presented by Susan Dana Jones and Sheila Magil BioProcess Technology Consultants www.bptc.com BioProcess Technology
More informationMass Spectra Alignments and their Significance
Mass Spectra Alignments and their Significance Sebastian Böcker 1, Hans-Michael altenbach 2 1 Technische Fakultät, Universität Bielefeld 2 NRW Int l Graduate School in Bioinformatics and Genome Research,
More information泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics
泛 用 蛋 白 質 體 學 之 質 譜 儀 資 料 分 析 平 台 的 建 立 與 應 用 Universal Mass Spectrometry Data Analysis Platform for Quantitative and Qualitative Proteomics 2014 Training Course Wei-Hung Chang ( 張 瑋 宏 ) ABRC, Academia
More informationSetting up a Quantitative Analysis MS ChemStation
Setting up a Quantitative Analysis MS ChemStation Getting Ready 1. Use the tutorial section "Quant Reports" on the MSD Reference Collection CD-ROM that came with your ChemStation software. 2. Know what
More informationMethods for Protein Analysis
Methods for Protein Analysis 1. Protein Separation Methods The following is a quick review of some common methods used for protein separation: SDS-PAGE (SDS-polyacrylamide gel electrophoresis) separates
More informationAbsolute quantification of low abundance proteins by shotgun proteomics
Absolute quantification of low abundance proteins by shotgun proteomics Dr. Stefanie Wienkoop www.proteomefactory.com In cooperation with: Max-Planck-Institut für Molekulare Pflanzenphysiologie Stable
More informationIntroduction to Proteomics
Introduction to Proteomics Åsa Wheelock, Ph.D. Division of Respiratory Medicine & Karolinska Biomics Center asa.wheelock@ki.se In: Systems Biology and the Omics Cascade, Karolinska Institutet, June 9-13,
More informationVTT TECHNICAL RESEARCH CENTRE OF FINLAND
Figure from: http://www.embl.de/nmr/sattler/teaching Why NMR (instead of X ray crystallography) a great number of macromolecules won't crystallize) natural environmant (water) ligand binding and inter
More informationBuilding innovative drug discovery alliances. Evotec Munich. Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs
Building innovative drug discovery alliances Evotec Munich Quantitative Proteomics to Support the Discovery & Development of Targeted Drugs Evotec AG, Evotec Munich, June 2013 About Evotec Munich A leader
More informationCorrelation of the Mass Spectrometric Analysis of Heat-Treated Glutaraldehyde Preparations to Their 235nm / 280 nm UV Absorbance Ratio
an ABC Laboratories white paper Correlation of the Mass Spectrometric Analysis of Heat-Treated Glutaraldehyde Preparations to Their 235nm / 280 nm UV Absorbance Ratio A. Sen, R. Dunphy, L. Rosik Analytical
More informationAxION edoor. Web-Based, Open-Access Mass Spectrometry Software
AxION edoor Web-Based, Open-Access Mass Spectrometry Software 2 AxION edoor is the open-access software solution for today s fast-paced laboratory. It is designed to operate and optimize the management
More informationIn-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types* S
Research In-depth Analysis of Tandem Mass Spectrometry Data from Disparate Instrument Types* S Robert J. Chalkley, Peter R. Baker, Katalin F. Medzihradszky, Aenoch J. Lynn, and A. L. Burlingame Mass spectrometric
More informationSpikeTides TM Peptides for relative and absolute quantification in SRM and MRM Assays
Protocol SpikeTides TM Peptides for relative and absolute quantification in SRM and MRM Assays Contact us: InfoLine: +49-30-6392-7878 Order per fax: +49-30-6392-7888 or e-mail: www: peptide@jpt.com www.jpt.com
More informationGuideline on similar biological medicinal products containing biotechnology-derived proteins as active substance: quality issues (revision 1)
22 May 2014 EMA/CHMP/BWP/247713/2012 Committee for Medicinal Products for Human Use (CHMP) Guideline on similar biological medicinal products containing biotechnology-derived proteins as active substance:
More informationAGILENT S BIOINFORMATICS ANALYSIS SOFTWARE
ACCELERATING PROGRESS IS IN OUR GENES AGILENT S BIOINFORMATICS ANALYSIS SOFTWARE GENESPRING GENE EXPRESSION (GX) MASS PROFILER PROFESSIONAL (MPP) PATHWAY ARCHITECT (PA) See Deeper. Reach Further. BIOINFORMATICS
More informationusing ms based proteomics
quantification using ms based proteomics lennart martens Computational Omics and Systems Biology Group Department of Medical Protein Research, VIB Department of Biochemistry, Ghent University Ghent, Belgium
More informationHow To Test For Contamination In Large Volume Water
Automated Solid Phase Extraction (SPE) of EPA Method 1694 for Pharmaceuticals and Personal Care Products in Large Volume Water Samples Keywords Application Note ENV0212 This collaboration study was performed
More informationSundar Ramanan, PhD, USA
GaBI Educational Workshops 2 3 March 2016, Hacettepe University, Ankara, Turkey First Turkish Interactive Workshop on Regulation and Approval of SIMILAR BIOTHERAPEUTIC PRODUCTS/BIOSIMILARS Sundar Ramanan,
More informationPreprocessing, Management, and Analysis of Mass Spectrometry Proteomics Data
Preprocessing, Management, and Analysis of Mass Spectrometry Proteomics Data M. Cannataro, P. H. Guzzi, T. Mazza, and P. Veltri Università Magna Græcia di Catanzaro, Italy 1 Introduction Mass Spectrometry
More informationAmgen Analytics of the Future Initiative Develops MS-based Multi-Attribute Methodology to Streamline Biotech Development and QC Testing
Amgen Analytics of the Future Initiative Develops MS-based Multi-Attribute Methodology to Streamline Biotech Development and QC Testing Amgen is among the major biotech companies working hard on empowering,
More informationPeak intensity Trypsin. Protein Sequence Before trypsin digestion After digestion with trypsin for 24 h digestion
Supplemental table 1. The completeness of trypsin digestion of the signature peptides with native flanking sequence. The signature peptides with native flanking sequence were digested with trypsin as described
More informationBrochure More information from http://www.researchandmarkets.com/reports/2172993/
Brochure More information from http://www.researchandmarkets.com/reports/2172993/ Proteomics Today. Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis,and Microarray Technology.
More informationPeptide Mapping 101: Essential Tools for Effective Development and Characterization. Part 1:Introduction to Peptide Mapping
Thank you for joining us! The Webinar will begin shortly Peptide Mapping 101: Essential Tools for Effective Development and Characterization Part 1:Introduction to Peptide Mapping Stephan M. Koza, Ph.
More informationA Generic Kit-Based Approach for Quantifying Monoclonal Antibody Drugs Through Direct Digestion of Discovery Study Samples
A Generic Kit-Based Approach for Quantifying Monoclonal Antibody Drugs Through Direct Digestion of Discovery Study Samples Mary Lame, Hua Yang, Sherri Naughton, and Erin Chambers Waters Corporation, Milford,
More informationThe Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring
The Scheduled MRM Algorithm Enables Intelligent Use of Retention Time During Multiple Reaction Monitoring Delivering up to 2500 MRM Transitions per LC Run Christie Hunter 1, Brigitte Simons 2 1 AB SCIEX,
More informationThermo Scientific BioLC Columns. Innovative solutions for mab analysis and characterization
Thermo Scientific BioLC Columns Innovative solutions for mab analysis and characterization Table of Contents Biopharma Terminology....................... 3 Introduction to Monoclonal Antibody (mab) Characterization...
More informationQ-TOF User s Booklet. Enter your username and password to login. After you login, the data acquisition program will automatically start.
Q-TOF User s Booklet Enter your username and password to login. After you login, the data acquisition program will automatically start. ~ 1 ~ This data acquisition window will come up after the program
More informationAppNote 6/2003. Analysis of Flavors using a Mass Spectral Based Chemical Sensor KEYWORDS ABSTRACT
AppNote 6/2003 Analysis of Flavors using a Mass Spectral Based Chemical Sensor Vanessa R. Kinton Gerstel, Inc., 701 Digital Drive, Suite J, Linthicum, MD 21090, USA Kevin L. Goodner USDA, Citrus & Subtropical
More informationimc FAMOS 6.3 visualization signal analysis data processing test reporting Comprehensive data analysis and documentation imc productive testing
imc FAMOS 6.3 visualization signal analysis data processing test reporting Comprehensive data analysis and documentation imc productive testing imc FAMOS ensures fast results Comprehensive data processing
More informationWhite Paper. Redefine Your Analytics Journey With Self-Service Data Discovery and Interactive Predictive Analytics
White Paper Redefine Your Analytics Journey With Self-Service Data Discovery and Interactive Predictive Analytics Contents Self-service data discovery and interactive predictive analytics... 1 What does
More informationPesticide Analysis by Mass Spectrometry
Pesticide Analysis by Mass Spectrometry Purpose: The purpose of this assignment is to introduce concepts of mass spectrometry (MS) as they pertain to the qualitative and quantitative analysis of organochlorine
More informationWelcome to today's webinar: How to Transform RMF & SMF into Availability Intelligence
Welcome to today's webinar: How to Transform RMF & SMF into Availability Intelligence The presentation will begin shortly Session Abstract: How to Transform RMF & SMF into Availability Intelligence It
More informationEnhancing GCMS analysis of trace compounds using a new dynamic baseline compensation algorithm to reduce background interference
Enhancing GCMS analysis of trace compounds using a new dynamic baseline compensation algorithm to reduce background interference Abstract The advantages of mass spectrometry (MS) in combination with gas
More informationThermo Scientific Prelude SPLC System FPO. Making LC/MS accessible. to clinical research and toxicology labs
Thermo Scientific Prelude SPLC System FPO Making LC/MS accessible to clinical research and toxicology labs Specifically designed to make the power of LC/MS accessible Analysis of target compounds in complex
More informationLC/MS Analysis of the Monoclonal Antibody Rituximab Using the Q Exactive Benchtop Orbitrap Mass Spectrometer
LC/MS Analysis of the Monoclonal Antibody Rituximab Using the Q Exactive Benchtop Orbitrap Mass Spectrometer Martin Samonig 1,2, Christian Huber 1,2 and Kai Scheffler 2,3 1 Division of Chemistry and Bioanalytics,
More informationMass Frontier Version 7.0
Mass Frontier Version 7.0 User Guide XCALI-97349 Revision A February 2011 2011 Thermo Fisher Scientific Inc. All rights reserved. Mass Frontier, Mass Frontier Server Manager, Fragmentation Library, Spectral
More informationWINDOWS AZURE AND WINDOWS HPC SERVER
David Chappell March 2012 WINDOWS AZURE AND WINDOWS HPC SERVER HIGH-PERFORMANCE COMPUTING IN THE CLOUD Sponsored by Microsoft Corporation Copyright 2012 Chappell & Associates Contents High-Performance
More information