MACHEREY-NAGEL MACHEREY MACHEREY-NAGEL -NAGEL
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1 Forensic science Forensics DNA extraction from forensic samples Excellent reliability Certified for forensic analysis Proven quality
2 MACHEREY-NAGEL Company profile Trusted quality since 1911 Since 1911 MACHEREY-NAGEL has established itself as a company for high quality, innovation, and reliability in biomolecular and chemical analysis. Friendly expert advice for our highly valued customers as well as outstanding product quality (EN ISO 9001:2008 and EN ISO 13485:2003 certified) have been the cornerstones of our corporate success for over 100 years. MACHEREY-NAGEL is a family-owned business run by the fourth generation, the great granddaughter of the company s founder, has been managing the enterprise since The companies headquarter is located in Düren, Germany. Today, the complete product portfolio includes Bioanalysis, Filtration, Rapid Tests, Water Analysis, and Chromatography. The Bioanalysis department provides innovative, client-driven solutions for DNA / RNA purification, clean-up, and concentration. Forensic quality products Consumables used in forensics need to be treated carefully to prevent DNA contamination. MACHEREY-NAGEL therefore has a stringently controlled production process to avoid DNA contamination of consumables. Further, MACHEREY-NAGEL uses ethylene oxide (EO) treatment to remove amplifiable DNA, which might still be introduced during the manufacturing process. MACHEREY-NAGEL products carrying the forensic quality seal, contain plastic materials that are EO treated. This means, DNA of any kind, which might still be introduced into plastic consumables during the production process, is inactivated by means of the treatment with EO, in order to prevent the generation of accidental human profile by PCR amplification. EO treatment has been shown to be the method of choice to prevent DNA profiles due to DNA contamination. (Shaw et al., 2008) UV Gamma Electron beam Ethylene oxide 100% 30% 40% 27% 3% 13% 87% Full profile 30% 70% Partial profile (loadable) Partial profile (unloadable) No profile Figure according to Shaw et al., 2008: Comparison of the effects of sterilization techniques on subsequent DNA profiling. Int J Legal Med 122:
3 NucleoSpin Forensic Filters Sample preparation Spin basket solution for lysis incubation and subsequent separation of lysate from samples such as swabs, denim, cigarette butts, etc. XXLysis and separation in one tube no sample transfer, no extra pipetting steps XXSpecial filter design absolutely leak proof, compatible with all typical lysis buffers XXComplete DNA recovery and process reliability, no DNA loss, no cross-contamination Technology Sample matrial Maximum volume capacity Forensic quality Special semi-permeable baskets Swabs, denim, cigarette butts, and other sample carriers 800 μl Ethylene oxide treated NucleoSpin Forensic Filters are the simplest and cleanest solution for lysis incubation and post-lysis separation of lysates from substrates. This unique product is especially designed for the forensic market. The NucleoSpin Forensic Filters enable the incubation of any solid sample carrier with any lysis buffer for any amount of time without leakage. Only a centrifugation step > 1 min at x g separates efficiently the solid carrier from the lysis buffer containing the desired DNA. The NucleoSpin Forensic Filters ensure a complete DNA recovery even from sample with only traces of DNA, without any cross-contamination. Prepare Incubate for lysis Spin for separation New product Processing swabs with the NucleoSpin Forensic Filters results in a significant increase of DNA yield! Product evaluation revealed up to 60 % higher recoveries in comparison to standard procedures. References and customer testimonials will follow shortly. NucleoSpin Forensic Filters 10 / 50 / /.50 /.250 NucleoSpin Forensic Filters (Bulk) 50 / 250 / * B /.250B / * * Larger bulk version available on request
4 NucleoMag Forensic DNA extraction DNA isolation for forensic sciences reliable quality for your STR analysis XXExcellent DNA purity from all types of casework samples XXMagnetic bead based isolation XXHigh sensitivity for low and high DNA content Technology Sample material Typical yield Elution volume Binding capacity Magnetic-bead technology Casework samples, contact traces (e.g., dried blood spots, cigarette filters, swabs) Depending on sample amount and quality μl 0.4 μg/μl beads The NucleoMag Forensic kit is designed for the reliable purification of DNA from different casework samples in forensic science (e.g., buccal swabs, blood spots, cigarette butts, chewing gum, epithelial cells, body fluids on fabric). The kit provides high quality DNA for your STR analysis from samples with high and low DNA content. The kit uses a magnetic bead based purification procedure, which can be used manually or automated on liquid handling platforms. The special lysis buffer allows the direct processing of different sample materials. Samples on a carrier should be separated after lysis using NucleoSpin Forensic Filters. DNA yield strongly depends on the DNA content in the specific sample (typical yield from buccal swab is ng). References STR profile obtained from cigarette butt Witt S, Neumann J, Zierdt H, Gébel G, Röscheisen C Establishing a novel automated magnetic bead-based method for the extraction of DNA from a variety of forensic samples* Forensic Sci Int Genet Sep;6(5): doi: /j.fsigen Epub 2012 Feb 5. LKA35 (DNA-Analytik), Landeskriminalamt Hamburg, Bruno-Georges-Platz 1, Hamburg, Germany. Le Balleur C 1), Brachet-Cota R 1), Marie O 1), Belrivo M-C 1), Shaad P 1), Carpentieri C 2) CSI: automated nuclear DNA extraction from rich, low traces using the NucleoMag Forensic kit on the Microlab STAR platform Hamilton Application Note 1) Institut national de police scientifique - Laboratoire de police scientifique de Marseille, 97 Bd Camille Flammarion, Marseille Cedex 4 2) MACHEREY-NAGEL France, 1 rue Gutenberg, Hoerdt. * Note: The described method is using MACHEREY-NAGEL products and led to the development of NucleoMag Forensic. NucleoMag Forensic 1 x 96 / 4 x /.4
5 NucleoSpin 8 Trace NucleoSpin 96 Trace DNA extraction Flexible 8-well and high throughput 96-well solution for gdna from trace samples X XTime-saving isolation of gdna from forensic samples (e.g., buccal swabs, dried blood spots, cigarette filters) X XVacuum or centrifugation procedure innovative MN Wash Plate minimizes the risk of cross-contamination XXDNA ready to use for any kind of enzymatic reaction, e.g., STR analysis Technology Sample material Typical yield Elution volume Binding capacity Silica-membrane technology Forensic samples, buccal swabs, blood spots, cigarette filters Depending on sample amount and quality μl 20 μg NucleoSpin 8 / 96 Trace kits are designed for the preparation of highly pure genomic DNA from small amounts of various tissue samples, cells, and forensic samples (e.g., buccal swabs, blood spots, cigarette filters). The extracted DNA can be used directly as template for PCR. The kits can be used manually under centrifugation or vacuum as well as on automated common liquid handling instruments. NucleoSpin 8 / 96 Trace Strip / Plate Customer testimonial The Landeskriminalamt Berlin (Berlin Crime Investigation Authority) is using NucleoSpin 8 / 96 Trace (MACHEREY-NAGEL) for extracting genomic DNA from forensic samples since We achieve satisfactory results with complete STR profiles from approximately 94 % of all buccal swabs in the first run and from another 4 % of all swabs by using a larger volume of the elution fraction in a second PCR. Up to now (August 2008) the throughput of buccal swab samples was in a range of more than 30,000 samples. Besides buccal swabs, DNA is also extracted from cigarette filters (more than 10,000 samples) and other trace materials by using the same system. Dr. Schelbert, Landeskriminalamt Berlin NucleoSpin 8 Trace 12 x 8 / 60 x /.5 NucleoSpin 96 Trace 2 x 96 / 4 x /.4
6 NucleoSpin Tissue NucleoSpin Tissue XS DNA extraction Mini Spin kits for extraction of genomic DNA XXHigh DNA recovery and purity XXSuitable for a huge variety of starting materials more than 16 optimized support protocols XXHigh sensitivity for DNA isolation from small sample amounts such as forensic samples NucleoSpin Tissue NucleoSpin Tissue XS Technology Silica-membrane technology Silica-membrane technology Sample material < 25 mg tissue, cells mg tissue, cells Typical yield μg (25 mg mouse liver) ng (0.025 mg mouse liver) ng (0.25 mg mouse liver) Elution volume μl 5 30 μl Binding capacity 60 μg 50 μg NucleoSpin Tissue is designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples (hair, dried blood spots, buccal swabs, cigarette filters), and clinical samples (stool, urine). NucleoSpin Tissue XS is designed for DNA isolation from very small samples such as microdissected tissue, small blood samples, or small amounts of cells. The high DNA yield of NucleoSpin Tissue XS and the small volume of only 5 30 μl elution buffer lead to highly concentrated DNA which is free of impurities and suitable for sensitive downstream applications. NucleoSpin Tissue Column NucleoSpin Tissue XS Column References NucleoSpin Tissue: Kondili A, Miniati P Prenatal samples used as DNA evidence in rape cases Forensic Science International: Genetics Supplement Series, Volume 3, Issue 1, December 2011, Pages e548-e549, Department of Biological Material Analysis, Division of Criminal Investigations, Hellenic Police, Sevastoupoleos 14 st., Athens, Greece. NucleoSpin Tissue XS: Färber D 1), Seul A 1), Weisser H-J 2) and Bohnert M 2) (2010) Recovery of Latent Fingerprints and DNA on Human Skin Journal of Forensic Sciences, 55: doi: /j x 1) German Federal Criminal Police, Wiesbaden, Germany. 2) University Hospital Freiburg Institute of Forensic Medicine, Albertstr. 9, Freiburg 79104, Germany. NucleoSpin Tissue 10 / 50 / /.50 /.250 NucleoSpin Tissue XS 10 / 50 / /.50 /.250
7 NucleoSpin gdna Clean up XS DNA clean-up Mini spin kit for post clean-up and concentration of small amounts of gdna XXEfficient post clean-up of large DNA fragments for successful downstream applications XXEasier and faster than microdialysis filtration units XXUnique XS spin column for low elution volume down to 6 μl highly concentrated DNA Technology Silica-membrane technology Sample material < 400 μl aqueous DNA solution (< 2 μg DNA) Typical recovery % Elution volume 6 10 μl Binding capacity 3 μg The NucleoSpin gdna Clean-up XS procedure is the easiest and fastest way to clean up small amounts of pre-purified largesized DNA following by a simple bind-wash-elute procedure. The proven silica membrane technology is an effective and fast alternative to the widely used Centricons for cleaning and concentrating samples, which were extracted by standard organic methods. The NucleoSpin gdna Clean-up XS Column allows very small elution volumes, resulting in highly concentrated DNA for downstream qpcr quantifications or STR amplifications. NucleoSpin gdna Clean-up XS Column Compared to ultrafiltration devices, sample preparation time is reduced from > 1 h to ~15 min. References Hudlow W-R, Krieger R, Meusel M, Sehhat J-C, Timken M-D, Buoncristiani M-R The NucleoSpin DNA Clean-up XS* kit for the concentration and purification of genomic DNA extracts: An alternative to microdialysis filtration Forensic Sci Int Genet Jun;5(3): Epub 2010 Apr 15. California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA. Faber K-L, Person E-C, Hudlow W-R PCR inhibitor removal using the NucleoSpin DNA Clean-up XS* kit Forensic Sci Int Genet Jan;7(1): Epub 2012 Jul 10. California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA. * Note: NucleoSpin DNA Clean-up XS has been renamed and is now available as NucleoSpin gdna Clean-up XS. NucleoSpin gdna Clean-up XS 10 / 50 / /.50 /.250
8 Sample preparation Preps REF NucleoSpin Forensic Filters 10 / 50 / /.50 /.250 NucleoSpin Forensic Filters (Bulk) 50 / 250 / * B /.250B / * DNA extraction Preps REF NucleoMag Forensic 1 x 96 / 4 x /.4 NucleoSpin 8 Trace 12 x 8 / 60 x /.5 NucleoSpin 96 Trace 2 x 96 / 4 x /.4 NucleoSpin Tissue 10 / 50 / /.50 /.250 NucleoSpin Tissue XS 10 / 50 / /.50 /.250 Genomic DNA clean-up NucleoSpin gdna Clean-up XS 10/50/ /.50/.250 * Larger bulk version available on request Related products: HTP consumables Material to be supplied by the user for NucleoMag Pack of REF E.g., Rack of Tube Strips (lysis tubes) 4 / /.24 Square-well Block 4 / /.24 E.g., Elution Plate U-bottom (elution plate) Product accessories for NucleoMag Pack of REF NucleoMag SEP NucleoSpin Trace Filter Plate KingFisher 96 Accessory Kit A (for use with KingFisher 96 platform) 1 set Product accessories for NucleoSpin Pack of REF NucleoVac 96 Vacuum Manifold NucleoVac Vacuum Regulator Starter Set A (for use of NucleoSpin 8-well strips on NucleoVac 96 Vacuum Manifold) Starter Set C (for use of NucleoSpin 8-well strips under centrifugation) NucleoSpin Trace Filter Plate Trademarks: NucleoSpin and NucleoMag are registered trademarks of MACHEREY-NAGEL; Centricon is a registered trademark of Millipore Corporation Your local distributor Image credits: maxkabakov - Fotolia.com KATEN / ForensicsGuide en2/2/0/ DD Printed in Germany MACHEREY-NAGEL EN ISO 9001: 2008 CERTIFIED MACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str Düren Germany Germany and international: Switzerland: MACHEREY-NAGEL AG France: MACHEREY-NAGEL EURL Tel.: Tel.: Tel.: Fax: Fax: Fax: info@mn-net.com sales-ch@mn-net.com sales-fr@mn-net.com USA: MACHEREY-NAGEL Inc. Tel.: Fax: sales-us@mn-net.com
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