EPA New England Regional Laboratory. EPA Project Team: Liz McCarthy. Matt Destino Tim Bridges Dick Siscanaw
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1 EPA s River Real Time Monitoring Buoy in the Charles and Mystic Watersheds Access to the data has been through a password protected site Tom Faber EPA New England Regional Laboratory EPA Project Team: Liz McCarthy Laura Senefeld Matt Destino Tim Bridges Dick Siscanaw
2 Charles and Mystic River Real-Time Monitoring EPA New England Regional Laboratory The Real-Time Monitoring Buoys are off-line for the season Purpose: Environmental Protection Agency (EPA) has established monitoring buoys in the Charles and Mystic Watersheds. These buoys collect and transmit water quality data that is available to the public. EPA has established these buoys to help with the tracking of cyanobacteria blooms and water quality conditions. Note: All water quality measurements are collected 1 meter below the water's surface Last Sonde Verification: 9/3/21 Click here Project Partners: Disclaimer: The data presented on this website is considered preliminary data and may be subject to future revision or qualifiers. The data from this site is transmitted directly from the instrument with no or little review. Inaccuracies may be presented because instrument malfunction or physical changes at buoy location.
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9 Sonde Measurements Recorded every 15 minutes Charles measurement period 5/27-9/3 Wedge Pond measurement period 6/15-9/3 Measurements collected at 1 meter depth Parameters Temperature, Conductivity ph Dissolved oxygen Turbidity (Charles only) Chlorophyll Phycocyanin
10 Buoy chlorophyll and phycocyanin y correlated with Lab data
11 Buoy chlorophyll and phycocyanin y correlated with Lab data Chlorophyll a EPA Contract lab DPH data (Charles only) Cyanobacteria cell counts EPA Contract lab DPH data (Charles only) Roger Frymire
12 probe (ug/l) Chlorophyll Chlorophyll probe had poor correlation with Laboratory data Chlorophyll a (EPA contract Lab) Vs. Chlorophyll Probe (with one outlier and data not meeting QC removed) Charles and Mystic data y =.3243x R 2 = Lab Chlorophyll a (ug/l) 4 Chlorophyll a (DPH contract lab) Vs. Chlorphyll probe Charles River data Prob be Chlorophyl (ug/l) y =.341x R 2 = DPH Chlorophyll a values (ug/l)
13 Phycocyanin probe correlation with Laboratory Cyanobacteria cell counts robe (cells/ml) Phycocyanin pr LSSU (EPA contract lab) cyanobacteria cell counts Vs Phycocyanin Probe With two outlier removed (Charles and Mystic data) y =.353x R 2 = LSSU cell counts (cells/ml) Phycocyanin prob be (cells/ml) Roger Frymirecyanobacteria cell counts Vs Phycocyanin Probe (Charles and Mystic data) 4 y =.69x R 2 = RF cell counts (cells/ml) lls/ml) Phycocyanin probe (cel 4, 35, 3, 25, 2, 15, 1, 5, DPH contract lab cyanobacteria cell counts Vs. Phycocyanin probe Charles River data y =.1364x R 2 = , 1, 15, 2, 25, 3, Total (DPH) Cell Count Phycocyanin probe (cells/m ml) Roger Frymire cyanobacteria cell counts Vs Phycocyanin Probe With high point removed (Charles and Mystic data) y =.555x R 2 = RF cell counts (cells/ml) Note: DPH samples collected ~35 feet away at dock 6 deep
14 Chalres RTM Buoy Phycocyanin Concentrations (cells/ml) ycocyanin Phy 5/27 6/3 6 6/17 6/24 7/1 7/8 7/15 7/22 7/29 8/5 8/12 8/19 8/26 9/2 9/9 9/16 9/23 9/ Date
15 Mystic RTM Buoy Phycyanin Concentrations Phycocyanin (cells/ml) 6/15/2 1 6/22/2 1 6/29/2 1 7/6/2 7/13/2 7/2/2 7/27/2 8/3/2 8/2 8/17/2 8/24/2 8/31/ /7/2 9/14/2 9/21/2 9/28/ Date
16 RTM Buoy Phyccyanin Concentrations (Mystic Data) Phycocyanin (cells/ml) 6/15 6/22 6/29 7/6 7/13 7/2 7/27 8/3 8 8/17 8/24 8/31 9/7 9/14 9/21 9/28 Date
17 Charles RTM Buoy Phycocyanin Corrected (w/dph values) Concentrations RTM Buoy Corrected DPH cell counts Phycocyanin (cell ls/ml) 5/27/ 6/3/ 6/ 6/17/ 6/24/ 7/1/ 7/8/ 7/15/ 7/22/ 7/29/ 8/5/ 8/12/ 8/19/ 8/26/ 9/2/ 9/9/ 9/16/ 9/23/ 9/3/ Date
18 Charles RTM Buoy Daily Max Phycocyanin corrected (DPH) values days above 7, 55 days equal to or below 7, /27 6/3 6 6/17 6/24 7/1 7/8 7/15 7/22 7/29 8/5 8/12 8/19 8/26 9/2 9/9 9/16 9/23 9/3 Date Phycocyanin ( cells/ml)
19 Other parameter comparisons with DPH and RTM Buoy DPH Dissolved Oxygen Vs. Optical Dissolved Oxygen Probe DPH ph Vs. ph Probe Optical DO (mg/l) RTM Buoy y =.3242x R 2 = DPH Dissolved Oxygen (mg/l) RTM Buoy ph 1 y = 1.339x R 2 = DPH ph RTM BUOY water Tempe rature (C) DPH Water Temperature Vs. Probe Water Temperature DPH Conductivity Vs. Probe Conductivity DPH Turbidity Vs. Probe Turbidity y =.8791x y = 1.36x R 2 =.9123 R 2 = y =.2436x R = DPHConductivity (us/cm) DPH Water Temperature (C) RTM Buoy Turbidity (NT TU) d. (us/cm) RTM Buoy Sp. Cond DPH Turbidity (NTU)
20 Charles Maximum Phycocyanin Concentrations 5 45 Phycocyanin n (cells/ml) : 3: 6: 9: 12: 15: 18: 21: : Time 7% of the Maximum daily values occurred between 6: pm and 6: am
21 9/16 9/23 9/ Charles RTM Buoy Dissolved Oxygen Concentrations 6 6/17 6/24 7/1 7/8 7/15 7/22 7/29 8/5 8/12 8/19 8/26 9/2 9/9 Date 6/1 6/3 5/27 5/2 DO (mg/l)
22 Charles RTM Buoy ph ph 5/ /27 6/3 6/ 6/ /17 6/ /24 7/1 7/ 7/ 7/ 7/8 /15 /22 /29 8/5 8/ /12 8/ /19 8/ /26 9/2 9/9 9/ /16 9/ /23 9/ /3 Date
23 Post deployment probe verification with Rhodamine dye 2 15 Phycocyanin Probe Performance (w/dye) Charles & Mystic 1 % Differe ence Chlorophyll Probe Performance (w/dye) Charles & Mystic 15 % Difference
24 Side by Sonde measurement during sonde swap percent Differen nce Relative Phycocyanin side by side Probe Performance -Charles & Mystic 549 Relative e percent Differe ence Chlorophyll side by side Probe Performance - Charles & Mystic
25 Next steps for RTM Buoy Project Evaluation user feedback was positive Most interest in tracking cyanobacteria blooms Relocating Mystic Buoy Plan on deploying again in the Charles and Mystic next summer Investigate t Public website Exchange Sondes every 2 weeks instead of weekly
26 Next steps for RTM Buoy Project Continued Investigate dff different calibration lb methods for chlorophyll and phycocyanin probes Use single dye standard d for both probes Phycocyanin Develop in-house ( (Microcystis aeruginosa) ) culture Initially correlated culture cell counts with dye concentration probe response Adjust probe calibration from most recent field sample counts Chlorophyll h ll Develop in-house fluorometric method for chlorophyll a (EPA Ref. method 445) Correlate chlorophyll a concentrations with dye concentration probe response Adjust probe calibration from most recent field sample chlorophyll a concentration
27 Questions?
Real-Time Monitoring Buoy in Mystic River Watershed
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