ESCCA 2015 SICILY 30 September - 3 October 2015

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1 ESCCA 2015 SICILY 30 September - 3 October 2015 On Wednesday afternoon and Thursday, 5 parallel courses will be combined with plenary sessions. A. Advanced hemato-oncology B. Diagnostica citometrica delle neoplasie ematologiche - corso di base C. RBC associated pathologies D. Flow cytometric methods in immunology E. Cell physiology, toxicology and microbiology On Friday and Saturday, several tracks of oral abstract presentations and workshops will alternate with plenary sessions. Pre-registration for 1 course is required. An application has been made to the EACCME for CME accreditation of Courses A, B, and C", as suggested by the Art. 55 of UEMS 2012/30 WEDNESDAY 30 SEPTEMBER A1 ADVANCED HEMATO-ONCOLOGY Welcome & Introduction of course topics and educational objectives Maria Arroz (Lisbon, PT) Diagnostic challenges in ALL patients post CAR therapy Maryalice Stetler-Stevenson (Bethesda, USA) B1 DIAGNOSTICA CITOMETRICA DELLE NEOPLASIE EMATOLOGICHE - CORSO DI BASE Welcome & Introduction of course topics and educational objectives Bruno Brando (Legnano, IT) Strategie citometriche in diagnostica ematologica I Claudio Ortolani (Urbino, IT) C1 RBC ASSOCIATED PATHOLOGIES Welcome & Introduction of course topics and educational objectives Chair: Janos Kappelmayer (Debrecen, HU) Erythroid and megakaryocytic lineage differentiation Marco Vitale (Parma, IT) D1 FLOW CYTOMETRIC METHODS IN IMMUNOLOGY Welcome & Introduction of course topics and educational objectives Claude Lambert (St. Etienne, FR) Immunophenotypic and functional assessment of antigen presenting cells Silvia Della Bella (Milan, IT) 1

2 E1 CELL PHYSIOLOGY, TOXICOLOGY AND MICROBIOLOGY Welcome & Introduction of course topics and educational objectives Enrique O Connor (Valencia, ES) Cell proliferation and cell cycle tools in anticancer research Paolo Cappella (Milano, IT) SHORT BREAK A2 ADVANCED HEMATO-ONCOLOGY Diagnosis and monitoring of CLL in the era of small molecule inhibitors Andy Rawstron (Leeds, UK) B2 DIAGNOSTICA CITOMETRICA DELLE NEOPLASIE EMATOLOGICHE - CORSO DI BASE La Citometria a Flusso nello studio delle neoplasie delle plasmacellule Bruno Brando (Legnano, IT) C2 RBC ASSOCIATED PATHOLOGIES Automated RBC parameters and red cell membrane abnormalities Zsuzsa Hevessy (Debrecen, HU) D2 FLOW CYTOMETRIC METHODS IN IMMUNOLOGY Analysing T cell subsets Claude Lambert (St. Etienne, FR) E2 CELL PHYSIOLOGY, TOXICOLOGY AND MICROBIOLOGY Intercellular communication and extracellular environment Francesca Luchetti (Urbino, IT) Barbara Canonico (Urbino, IT) BREAK PLENARY 1 WELCOME ADDRESSES & KEYNOTE LECTURE Chair: Bruno Brando (Milan, IT) Words of Welcome - Claude Lambert (Saint-Etienne, FR) - Stefano Papa (Urbino, IT) - Bruno Brando (Milan, IT) PLE-1 Human NK cells: from receptors to the cure of high risk leukemia Lorenzo Moretta (Genoa, IT) ESCCA INDUSTRIAL PARTNER PRESENTATION CORPORATE LECTURE (to be confirmed) WELCOME RECEPTION - OPENING OF THE EXHIBITION 2

3 THURSDAY 1 OCTOBER PLENARY 2 FLOW CYTOMETRY ON THE MOLECULAR LEVEL Chair: Maria Stetler-Stevenson (Bethesda, USA) PLE-2-01 Phosflow in clinical diagnostics Andy Rawstron (Leeds, UK) PLE-2-02 mrna flow cytometry Paul Wallace (Buffalo, USA) ESCCA INDUSTRIAL PARTNER PRESENTATION BREAK - POSTERS - EXHIBITION A3 ADVANCED HEMATO-ONCOLOGY MRD in MM Maryalice Stetler-Stevenson (Bethesda, USA) Maria Arroz (Lisbon, PT) B3 DIAGNOSTICA CITOMETRICA DELLE NEOPLASIE EMATOLOGICHE - CORSO DI BASE La Citometria a Flusso nello studio delle malattie linfoproliferative croniche Donatella Raspadori (Siena, IT) C3 RBC ASSOCIATED PATHOLOGIES - PNH session Introduction to PNH Massimo Geuna (Turin, IT) Clinical/laboratory classification of PNH Elisa Cannizzo (Oristano, IT) Diagnostic tests for PNH Elisa Cannizzo (Oristano, IT) D3 FLOW CYTOMETRIC METHODS IN IMMUNOLOGY Flow Cytometry in the study of cell degranulation Genny del Zotto (Genoa, IT) E3 CELL PHYSIOLOGY, TOXICOLOGY AND MICROBIOLOGY New Approaches: Real-Time Cytometry and Imaging Flow Cytometry Enrique O Connor (Valencia, ES) LUNCH BREAK - POSTERS - EXHIBITION A4 ADVANCED HEMATO-ONCOLOGY Dissection of normal vs aberrant maturation pathways in the bone marrow Alberto Orfao (Salamanca, ES) 3

4 B4 DIAGNOSTICA CITOMETRICA DELLE NEOPLASIE EMATOLOGICHE - CORSO DI BASE La Citometria a Flusso nello studio dei campioni non convenzionali Arianna Gatti (Legnano, IT) C4 RBC ASSOCIATED PATHOLOGIES - PNH session PNH: reasons for testing Massimo Geuna (Turin, IT) The Italian "Clone PNH archive" experience Massimo Geuna (Turin, IT) International recommendations Elisa Cannizzo (Oristano, IT) D4 FLOW CYTOMETRIC METHODS IN IMMUNOLOGY Cell proliferation in the assessment of immune function Silvia Della Bella (Milan, IT) E4 CELL PHYSIOLOGY, TOXICOLOGY AND MICROBIOLOGY Microbiology: Current Protocols Anita Manti (Urbino, IT) Stefano Papa (Urbino, IT) BREAK - EXHIBITION - POSTERS A5 ADVANCED HEMATO-ONCOLOGY Next Generation Flow (NGF): towards a data base guided immunophenotypic diagnosis and classification of haematological malignancies Alberto Orfao (Salamanca, ES) B5 DIAGNOSTICA CITOMETRICA DELLE NEOPLASIE EMATOLOGICHE - CORSO DI BASE Strategie citometriche in diagnostica ematologica II Claudio Ortolani (Urbino, IT) C5 RBC ASSOCIATED PATHOLOGIES Hemostasis abnormalities in red cell disorders Janos Kappelmayer (Debrecen, HU) D5 FLOW CYTOMETRIC METHODS IN IMMUNOLOGY Panel s choice in the study of the immune system: a practical approach Genny del Zotto (Genoa, IT) 4

5 E5 CELL PHYSIOLOGY, TOXICOLOGY AND MICROBIOLOGY Cytomics in Predictive Toxicology Enrique O Connor (Valencia, ES) CONFERENCE DINNER Pre-registration required; a fee applies. FRIDAY 2 OCTOBER PLENARY 3 ACCREDITATION SYSTEMS IN (FLOW) CYTOMETRY Chair: Silvia Della Bella (Milan, IT) PLE-3-01 Accreditation in Cytometry: where are we going? Paul Wallace (Buffalo, USA) PLE-3-02 Test Yourself in Clinical Cell Analysis (the TYsCCA Project) Claude Lambert (Saint-Etienne, FR) PLE-3-03 ISAC/ICCS Certification and Education Programs Paul Wallace (Buffalo, USA) PLE-3-04 How to seek and find practical help in Clinical Cell Analysis (the ESCCA base project) Claudio Ortolani (Urbino, IT) ESCCA INDUSTRIAL PARTNER PRESENTATION CORPORATE LECTURE Exhibitor Raffle Prize drawing BREAK - POSTERS - EXHIBITION PARALLEL 1 PNH Chair: Massimo Geuna (Turin, IT) PAR-1-01 PNH clone detection Frank Preijers (Nijmegen, NL) PAR-1-02 Selected oral abstract presentation PAR-1-03 Selected oral abstract presentation PAR-1-04 Selected oral abstract presentation PAR-1-05 Selected oral abstract presentation 5

6 PARALLEL 2 AML AND ITS MRD Chair: Giuseppe Basso (Padua, IT) PAR-2-01 MRD in AML stem cells Gerrit Jan Schuurhuis (Amsterdam, NL) PAR-2-02 Selected oral abstract presentation PAR-2-03 Selected oral abstract presentation PAR-2-04 Selected oral abstract presentation PAR-2-05 Selected oral abstract presentation PARALLEL 3 IMAGE CYTOMETRY Chair: Enrique O Connor (Valencia, ES) PAR-3-01 Quality control in image cytomety Attila Tarnok (Leipzig, DE) PAR-3-02 Selected oral abstract presentation PAR-3-03 Selected oral abstract presentation PAR-3-04 Selected oral abstract presentation PAR-3-05 Selected oral abstract presentation PARALLEL 4 IMMUNOLOGIA (ITALIAN SESSION) Chair: Cristina Mingari (Genoa, IT) PAR-4-01 La Citometria a Flusso nella Diagnostica: un aiuto moderno per gli Immunologi Clinici Andrea Cossarizza (Modena, IT) PAR-4-02 Selected oral abstract presentation PAR-4-03 Selected oral abstract presentation PAR-4-04 Selected oral abstract presentation PAR-4-05 Selected oral abstract presentation WORKSHOP 1 PLATELETS AND MICROPARTICLES Chair: Stefano Papa (Urbino, IT) WS-1-01 Exosomes and other extracellular vesicles: counting and characterization Barbara Canonico (Urbino, IT) WS-1-02 Cell derived extra cellular vesicles : emergent diagnostic and prognostic biomarkers Françoise Dignat-George (Marseille, FR) WS-1-03 Direct and indirect tests of platelet activation Janos Kappelmayer (Debrecen, HU) Discussion LUNCH BREAK - POSTERS - EXHIBITION 6

7 PARALLEL 5 ALL AND ITS MRD Chair: Alberto Orfao (Salamanca, ES) PAR-5-01 MRD flow cytometry in AIEOP-BFM clinical trials Giuseppe Basso (Padoa, IT) PAR-5-02 Selected oral abstract presentation PAR-5-03 Selected oral abstract presentation PAR-5-04 Selected oral abstract presentation PAR-5-05 Selected oral abstract presentation PARALLEL 6 FLOW CYTOMETRY IN THE B AND NK CELL LINEAGE Chair: Andrea Cossarizza (Modena, IT) PAR-6-01 Memory B cell subset detection by flow Rita Carsetti (Rome, IT) PAR-6-02 Selected oral abstract presentation PAR-6-03 Selected oral abstract presentation PAR-6-04 Selected oral abstract presentation PAR-6-05 Selected oral abstract presentation PARALLEL 7 MASS CYTOMETRY Chair: Claude Lambert (St. Etienne, FR) PAR-7-01 Immunological map of HIV infection by mass cytometry Antonio Cosma (Paris, FR) PAR-7-02 Selected oral abstract presentation PAR-7-03 Selected oral abstract presentation PAR-7-04 Selected oral abstract presentation PARALLEL 8 ONCOEMATOLOGIA (ITALIAN SESSION) Chair: Donatella Raspadori (Siena, IT) PAR-8-01 Standardizzazione del fenotipo nella AML, dalla diagnosi alla determinazione della prognosi Francesco Buccisano (Rome, IT) PAR-8-02 Analisi dei profili di signalling nella leucemia linfatica cronica Maria Teresa Scupoli (Verona, IT) PAR-8-03 Selected oral abstract presentation PAR-8-04 Selected oral abstract presentation 7

8 WORKSHOP 2 DENDRITIC CELLS IN HEALTH AND DISEASE Chair: Bruno Brando (Legnano, IT) WS-2-01 DCs in physiologic and pathologic human pregnancy Silvia Della Bella (Milan, IT) WS-2-02 DCs in cancer Daniel Olive (Marseille, FR) WS-2-03 The Plasmacytoid Dendritic Cell a key actor in autoimmune diseases Lars Rönnblom (Uppsala, SE) WS-2-04 The role of DCs in infections Katherina Psarra (Athens, GR) Discussion and concluding remarks BREAK - POSTERS - EXHIBITION CLINICAL CYTOMETRY BEST PAPER AWARD Chair: Stefano Papa (Urbino, IT) The nine Associate Editors of Clinical Cytometry will select the best paper among the original papers that have been published in the journal between May 2014 and March The award will be presented to the winner by the Editor-in-Chief, Dr. Fred Preffer PLENARY 4 MICROBIOLOGY IN FLOW Chair: Stefano Papa (Urbino, IT) PLE-4-01 Remote control of epithelial cells on the gut microbiota Mathias Chamaillard (Lille, FR) ESCCA GENERAL ASSEMBLY SATURDAY 3 OCTOBER PARALLEL 9 MRD IN B-LYMPHOPROLIFERATIVE DISORDERS Chair: Frank Preijers (Nijmegen, NL) PAR-9-01 MRD in CLL Andy Rawstron (Leeds, UK) PAR-9-02 Selected oral abstract presentation PAR-9-03 Selected oral abstract presentation PAR-9-04 Selected oral abstract presentation PAR-9-05 Selected oral abstract presentation 8

9 PARALLEL 10 FLOW CYTOMETRY IN THE T CELL LINEAGE Chair: Claude Lambert (St. Etienne, FR) PAR Flow Cytometry for the identification of rare T cells Andrea Cossarizza (Modena, IT) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PARALLEL 11 TUMOR CELLS Chair: Zsuzsana Hevessy (Debrecen, HU) SCHEDULING OF THIS SESSION IS IN PROGRESS More details will follow in the next update of the programme! PARALLEL 12 LEUCEMIE ACUTE E MALATTIA MINIMA RESIDUA (ITALIAN SESSION) Chair: Giuseppe Basso (Padoa, IT) PAR Miti e controversie della monitorizzazione della MRD nella Leucemia Acuta Mieloide Francesco Buccisano (Rome, IT) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation WORKSHOP 3 MEET THE COURSE ORGANIZERS with Maria Arroz, Janos Kappelmayer, Claude Lambert, Enrique O Connor, Claudio Ortolani (for the Italian Course) More details will follow in the next update of the programme! BREAK PLENARY 5 IMMUNOLOGY OF CANCER Chair: Gerrit Jan Schuurhuis (Amsterdam, NL) PLE-5-01 Cancer Immunology Daniel Olive (Marseille, FR) PLE-5-02 The CD38 family (CD38 and CD157 as modulator of migration and immune response) Fabio Malavasi (Turin, IT) LUNCH BREAK 9

10 PARALLEL 13 STEM CELLS AND GENE THERAPY Chair: Massimo Geuna (Turin, IT) PAR Flow cytometry for the Quality Control of advanced medicinal products Margherita Neri (Milan, IT) PAR Patient derived ipsc and gene therapy: a new approach for hematological diseases? Jan Philippé (Ghent, BE) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PARALLEL 14 MYELOID SUPPRESSOR CELLS Chair: Katherina Psarra (Athens, GR) PAR Myeloid Suppressor Cells in Multiple Myeloma Jo van Ginderachter (Brussels, BE) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PARALLEL 15 FLOW CYTOMETRY IN MYELOID DEVELOPMENT Chair: Maria Arroz (Lisbon, PT) PAR Human monocytic differentiation pathways in the bone marrow and peripheral blood Alberto Orfao (Salamanca, ES) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PARALLEL 16 IL MIELOMA MULTIPLO E LA SUA MRD (ITALIAN SESSION) Chair: Claudio Ortolani (Urbino, IT) PAR Diagnosi e Follow-Up del Mieloma: Go With The Flow Bruno Brando (Milan, IT) PAR MRD e Mieloma: Che differenze tra plasmacellule "normali" e "patologiche" Francesco Gervasi (Palermo, IT) PAR Selected oral abstract presentation PAR Selected oral abstract presentation 10

11 PARALLEL 17 YOUNG (PhD) CYTOMETRISTS ORAL ABSTRACT PRESENTATIONS Chair: Zsuzsana Hevessy (Debrecen, HU) PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation PAR Selected oral abstract presentation BREAK PLENARY 6 METHODS FOR FLOW CYTOMETRY DATA ANALYSIS Chair: Jan Philippé (Ghent, BE) PLE-6-01 Polychromatic flow cytometry of complex T cell phenotypes Enrico Lugli (Milan, IT) PLE-6-02 Integrating single-cell technologies for high-resolution analysis of immunity in health and disease Iyadh Douagi (Stockholm, SE) AWARDS & CLOSURE Chair: Claude Lambert (St. Etienne, FR) Presentation of ESCCA 2016 Announcement of the winners of the - ESCCA Best Oral Abstract Award - ESCCA Best Poster Award Closing remarks FAREWELL COCKTAIL AT THE BEACH Pre-registration required; a fee applies. Limited places available! 11

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