1 Eradicating malaria and diarrhoea Pierre Lutgen, docteur en chimie, Lussemburgo Roma 23, aprile 2010
2 The resistance of the parasites to conventional drugs (Chloroquine, Mefloquine, Amodiaquine) has accelerated (up to 50 % resistance) and these drugs have serious side effects
3 The lobby of organophosphate and permethrin producers by all means prevents to use of DDT-IRS in prevention - despite the recommendation of WHO 16 sept despite the notification procedure of the Stockholm Convention on POPs - despite the fact that DDT is the only insecticide wihout any effect on human health
4 The only strategy left?
5 New healing possibility Tea of Artemisia annua Efficiency 90% No side effects Low cost No resistance shown up til today
6 The healing power was rediscovered during the Vietnam war by the Chinese
7 The pharmaceutical companies combine artemisinine, one of the active substances contained in the plant, with old antimalarials like mefloquine or amodiaquine: Artemisia combined therapy (ACT)
8 But ACTs cause negative health effects: (red lumefantrine, black mefloquine) R.Hutagalung et al., Malaria Journal, 4, 48, 2005 Artemisia annua tea has none
9 Infusion of Artemisia annua Infuse dry leaves in very hot water (no boiling) Use 50 gr of leaves spread over at least 20 cups of fresh tea during 7days to avoid recurrence. We refuse to supply further samples of tea to those who do not comply with this procedure
10 Artemisinin, the active substance in the plant, is an organic peroxide. It kills Plasmodium through OH radicals in the Fenton reaction in combination with iron. It is a chemical, not a biological process, which has not allowed the mosquitos to become resistant in 3000 years. But the plant also contains polyphenols and flavonoids which are active against malaria and synergize with artemisinin Artemisia annua tea is a polytherapy, not a monotherapy
11 Artemisia annua from Luxembourg is dried in an oven at 35 C during 3 days.
12 Flavonoïds and other substances present in the tea multiply the efficacity of artemisinine 2 to 5 x Elford et al.,trans R Soc Tro Med Hyg 1987, 8, K Chiung.Sheue et al., Plant Cool Reports, 1992, 11,
13 We were pleased to learn about these clinical trials last year OPTIMIZING THE TREATMENT OF UNCOMPLICATED MALARIA USING TEA OF HYBRID ARTEMISIA ANNUA Adelaide Bela Agostinho, Ariadna Vlyalko, João Massingarela and João Fumane Roma, 13 th Mar 2009
14 Results presented in January to Dr Mario Dicato and Dr Pierre Lutgen by Dr Emilien Fouda at Yaoundé, Clinique Cité Verte, Cameroun concerning 72 patients 14
16 COMPARATIVE STUDY OF THE QUALITY AND EFFICIENCY OF ARTEMISININ DRUG BASED AND ARTEMISIA ANNUA GROWN IN CAMEROON [MIM ] Chougouo Kengne R.D*., Kouamouo J*., Moyou Somo R**., Penge On Okoko A***. *Université des Montagnes Cameroun ; ** IMPM /CRM et FMSB Cameroun ; *** Université de Kinshasa RDC. The Western part of Cameroon has 33% of counterfeit drugs in the market and Artemisia annua is a plant known for its antimalarial effect because containing artemisinin. In order to compare the antimalarial activity of artemisinin from Artemisia annua and other antimalarial drugs: artesunate and artesunate + amodiaquine. The results of the comparative study showed a significantly higher sensitivity of artemisia annua concoction (0 % of Echec Thérapeutique Tardif ETT) compared with that of artesunate (12.5% of ETT) and the artesunate combined with amodiaquine (14.30% of ETT). The concoction intake for 7 days was 0% of ETT, significantly lower than that of 5 days intake (28.5%). Like RTPA, the bitherapy had a higher sensitivity (85.70%) than mono-therapy (81.25%). The artemisinin level in A. annua in Cameroon was 1.3%. The RTPA is above 80% for the 3 protocols indicating the absence of warning according to WHO criteria. The concoction of Artemisia annua is a good treatment of malaria seeing the results. To improve its effectiveness, it must be taken for at least 7 days or in combination with other antimalarial drugs.
17 Etude in vitro de la chimiosensibilité des souches de Plasmodium falciparum par rapport à la tisane d Artemisia annua comparée aux principaux antimalariques utilisés au Sénégal Papa Elhadji Omar GUEYE Doctorant en Biologie et Pathologie Humaine. Université Cheikh Anta Diop Sénégal Project financed by ArcelorMittalFoundation
19 Non-conformity with procedure of certain trials may also inform us on the potential of artemisia annua tea from Luxembourg. Somebody in Ghana had received 8 bags of 50 g and used them on 40 patients-children by preparing for them a litre of tea which they took home to drink over 3 days. They were all cured!
20 One failure: Our partner in Namibia run out of dried herb, and used a few weeks old green plants for preparing artemisia annua tea. Disappointed!
21 Culture of Artemisia annua at Walferdange-Luxembourg, dried and packed at Téi vum Séi in professional equipement
22 With seeds from Luxembourg for every garden in Africa. Actually 49 partners in 20 countries. List available
23 Our plantations in Peru
24 Our plantations in Congo
25 The Lycée Technique de Thiès in Sénégal will cultivate seedlings of Artemisia annua for the entire region in collaboration with Irdelle Lagnide of RC Luxembourg-Schuman
26 But there are many other applications or end-uses for artemisia annua These are not our corebusiness, but they may help make artemisia annua better known
27 Site of Action Artemisinin Conventional Treatment Artemisinin
28 Artemisinin not only cures by killing plasmodia in the blood, but also inhibits transmisssion from man to mosquito by killing the gametocytes Complete loss of mosquito infectivity 24 h after artemisinin injection GP Dutta et al., Chemotherapy, 35, 200, 1989 Widespread introduction of artemisinin derivates may prevent spread of multidrug resistance RN Price et al., Lancet, 15, 1654, 1996 Artemisinin is much better in blocking transmission of malaria than mefloquine PQ Chen et a., Chin Med J., 107, 709, Artemisinin kills the early stages of gametocytes of P.falciparum N H Kumar et al., Parasitol Res., 76, 214, 1990.
29 Ligne pointillée grasse : artesunate seul beaucoup plus efficace pour absence de gametocytes Ligne pointillée fine: artesunate-amodiaquine Ligne pleine : amodiaquine A Sowunmi et al, AAC, 51, 1694, 2007 If artesunate is so much more gametocytocidal than other drugs, what about artemisia annua tea?
30 Our partners in Peru discovered (or rediscovered a Chinese technique) : fumigation with artemisisia annua efficiently repels mosquitoes from dormitories. Our partners at the university in Medellin study artemisia annua cream as a repellent.
31 Scientists from IFBV have discovered that artemisia annua tea sterilizes water Stenay- Metz RLU Untreated contaminated water Contaminated water + UV Contaminated water boiled Contaminated water + 20% tea Contaminated Water + 50% tea
32 Action de l'artémisia Annua sur les Coliformes Fécaux RLU Stenay- Metz UFC/100 ml 6 4 UFC 5% UFC 10% UFC 20% UFC 20% 0 0 0h 1h Temps de Contact en heure 5h 24h UFC 5% UFC 10% Résultats de Bangui sur E.coli avec trois tisanes A annua de concentration différente Addition of 1ml of a Pseudomonas aeruginosa culture to Artemisia annua tea Untreated contaminated water Contaminated water + UV Contaminated water boiled Contaminated water + 20% tea Contaminated Water + 50% tea Différents traitements de Luxembourg-LCDI sur eau de station d épuration (tisanes à droite) Crecimiento de Coliformes totales y fecales RLU Rijksuniversiteit- Gent- Belgium 0.5 g tea/ 50 ml UFC/100ml Coliformes fecales Coliformes totales time (hours) Control 1 Control 2 SODIS 1 SODIS 2 Artemisia 1 Muestra Artemisia 2 SODIS + Art. 1 SODIS + Art. 2 Universidad de Antioquia, Colombia Dans quatre essais fort différents, la tisane d artemisia annua montre un effet marquant et inattendu sur la concentration de bactéries dans l eau.
33 Combining this with SODIS the solar desinfection of water, a technique which which our ONG is implementing in 8 countries, a large number of the children which die every day of «dioerrhea» could be saved
34 the same scientists from IFBV have discovered that artemisia annua tea is the only tea fluorescent under black UV light But after a few hours under sunlight the fluorescence disappears. Why?
35 In vitro study of cytotoxic effect of Artemisia annua tea 1 Zorica Juranić, 2 Pierre Lutgen, 1 Ivana Matić, 3 Milan Juranić 1 Institute of Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia 2 IFBV, 10 rue Kommes L-6988, Hostert, Luxembourg Poster for EACR conference, Oslo 2010 Results. Tea prepared from Artemisia annua dry leaves exhibited selective dose-dependent cytotoxic effect against malignant cell lines and both unstimulated and stimulated PBMC. The strongest cytotoxic action was observed against K562 cells (IC 50 = 1.33±0.38 mg/ml). Moreover, tea exerted pronounced cytotoxic effect on human malignant melanoma BG and Fem-x cells (IC 50 =3.20±0.65 and IC 50 =3.76±1.20 mg/ml respectively), and HeLa cells, which showed similar sensitivity to investigated agent (IC 50 =3.06±0.62 mg/ml). Cytotoxic activity was found to be weaker against MDA-MB-361 and LS174 cells (IC 50 =8.86±0.42 and IC 50 =10.45±0.26 mg/ml respectively). Conclusions. The present research clearly demonstrates stronger and selective (Ksel>6.95) antitumor effect of Artemisia annua tea to leukemia K562 cells in comparison to healthy PBMC.
36 We study synergy between curcumin and artemisinin on inflammation at the Laboratoire de Biologie Moléculaire et Chimie du Cancer at Luxembourg, Or the synergy between moringa and artemisia annua on prostate cancer at the Clinique de la Cité Verte in Yaoundé
38 04.janv.10 Cell kill effect MCF7 cellules cancer du sein, tisane de Luxembourg et du Brésil Dr. Ning Wang, CRP-Sant, Luxembourg
39 With Pr Ivan Velez, Medellin, Colombia and expert at the WHO, Geneva we are investigating the therapeutic effect of artemisia annua on leishmaniosis
40 Artcream Based on an ethanolic extract of artemisia annua Very efficient against skin diseases, woundhealing. Developed by B Michels, IFBV. Trials in Bangui and Medellin
41 Fasciola hepatica is a disaster for cattle in the Andes. Artemisia annua is very efficicient against this liver parasite. Our partners in Colombia and Peru in a joint effort try to implement the technique
42 Artemisia residues are useful in agriculture
43 ACT pills: A programmed disaster?
44 Since 3 years resistance to ACT s has been observed in several countries; Cambodia, Senegal, Thailandia, Burundi, Colombia, Sierra Leone New study confirms growing antimalarial resistance "If we lose artemisinin combination therapy, that will be a disaster for malaria control in the whole world. No one knows the mechanism of resistance" Dr Charles Dellacollette, WHO Also Dr Tido von Angerer Médecins sans Frontières raises alarm Lancet, 13 April 2009 New England Journal of Medicine, (2009)
45 In many countries more than 50 % of all pills sold on the market are counterfeit. A crime and disaster. Newton PN, et al. (2006) Manslaughter by Fake Artesunate in Asia Will Africa Be Next? PLoS Med 3(6): Genuine (left), Counterfeit (right). Artesunate, Cameroon.
46 In most of African countries the use of ACTs remains low, 3% of the children on the average, from 0.1 % in Gambia à 13 % in Zambia. World Malaria Report. Sept 2008
48 is a small NGO of engineers and scientists: Two rotarians contribute to the core scientific team Dr Mario Dicato and Ph.D. Pierre Lutgen, RC Luxembourg Vallées In cooperation with the universities of Yaoundé, Bangangté, Bangui, Dakar, Lubumbashi, Campinas, Medellin, Cajamarca, Louvain And several research laboratories in Luxembourg
49 80% of the world population only can afford traditional herbal medicine, often very efficiently. Let them do it!
50 Research and academic partners. Universities of Bangui, Dakar, Lubumbashi, Medellin, Campinas, Cumana, Yaoundé, Louvain Research Centres and Laboratories in Luxembourg : CRP-Santé, CRP- Lippmann, LBMCC, Laboratoire National de la Santé
51 Message delivered by Rolf Tarrach, RC Luxembourg Vallées to IFBVs 1st Congress «Tropical diseases» at Luxembourg Le Congrès de IFBV veut se concentrer sur des procédures sanitaires et de gestion sociale simples, accessibles, abordables, faciles à réaliser dans les conditions du tiers monde, mais il y aura aussi quelques nouvelles thérapeutiques. Bravo! Allez-y, soyez créatifs et persistants et sûrement un jour nous y arriverons! Rolf Tarrach Recteur Université de Luxembourg
52 Cost per treatment per patient per year HIV/AIDS: 800 TB : 650 Malaria (A.annua) : 0,5-2 *
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