Agri Livestock Asia. 5th International conference Karachi April Alain Réocreux Olmix France Orient Animal Health
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1 Agri Livestock Asia 5th International conference Karachi April Alain Réocreux Olmix France Orient Animal Health
2 Nanotechnology M T.X+ Toxin Binder in Livestock Industry and farm applications Eco Concept M Feed Natural Growth Promoter
3 a b EUROPEAN RESEARCH PROGRAM «EUREKA» Amadeite In NANOTECHNOLOGY c Discovered in 2005 by Olmix in France
4 Amadeite, Amadeite, the origins to his its industrial applications Why using Montmorillonite? oxygen silicon a b c hydroxyl or oxygen aluminium and magnesium Na + Ca 2+ Na + Net negative charge of the layers Ca 2+ Na + Ca 2+ Interlayer cations
5 Amadeite, Amadeite, the origins to his its industrial applications Why using biopolymers extrated from seaweeds? Seaweeds
6 Amadeite, Amadeite, the origins to his its industrial applications Why using clays and biopolymers extrated from seaweeds? Na + Ca 2+ Ca 2+ Na + Na + Ca 2+ + biopolymers montmorillonite Specific biding of big size mycotoxins such as Fumonisines, Trichothecenes and zearalenone
7 THE MT.X+ INNOVATION The non-chemical intercalation or how to increase the space between the layers with a natural material. A revolutionary process A natural agent SEAWEEDS 7
8 THE MT.X+ INNOVATION The layers intercalation with a seaweed extract Space between layer multiplied by 10! INTERCALATED CLAY 8
9 There is a reason for increasing the interlayer space Removal of the limiting factor Opening of the interlayer spaces allowing the entrance of the big size mycotoxins in the structure and their binding by adsorption on the internal surfaces. A wide spectrum of adsorption NO with standard clay YES with Nanotechnology MT.X+
10 WHY THE CLAYS? THE ASPECT RATIO The developed surface by structural unit MACROMETRIC SCALE 1 CM² PER GRAM 10
11 WHY THE CLAYS? THE ASPECT RATIO The developed surface by structural unit MICROMETRIC SCALE Magnification x m² PER GRAM 11
12 THE MT.X+ INNOVATION = NANOTECHNOLOGY THE ASPECT RATIO The developed surface by structural unit NANOMETRIC SCALE Magnification x From 1 cm2 to 800 m² PER GRAM 12
13 Contamination level worldwide
14 Mycotoxins doesn t end in the feed! Transferred into meat, eggs, milk Decrease quality of food ( changes in lipid profiles, antioxidant concentrations ) Affect blood composition JE Dvorska Veterinary Medicine department University Ukraine-WPC July 2008 Australia
15 In vitro tests Binding capacity of MTox Plus on Aflatoxins Contamination level 1 µg / mg of MTOX 2,5 µg / mg of MTOX 5 µg / mg of MTOX AF B1 AF B2 AF G1 AF G2 99, , ,5 99, Ref : LDA 22 France 2005 Trial protocol : Mise en solution dans un mélange KCl : 0,05 M HCl 0,013 M ( V/V ) à ph 2,35 des différentes mycotoxines afin de réaliser une gamme de concentration à 3 niveaux de l ordre de 10 à 50 µg/ml par molécule. 110 mg de chaque support est mis en contact avec 1 ml ces différents niveaux de concentrations dans un tube conique. Après agitation au vortex, ces tubes sont placés à l étuve à 38 + / - 1 C pendant 1 heure sous agitation par retournement Après remise à température ambiante, centrifugation à 1500 Tr / mn pendant 5 mn Injection de 10 µl du surnageant dans un système de Chromatographie Liquide Haute Performance ( HPLC ) avec détection Fluorimétrique ( selon normes AFNOR ). Identification et Quantification des molécules retrouvées dans le surnageant
16 In vitro tests Binding capacity of MTox+ on DON and Zearalenone DON Intestinal adsorption % MTox + batch 1 59 MTox + batch 2 61 MTox + batch 3 65 Zearalenone Intestinal adsorption % MTox + batch 1 62 MTox + batch 2 72 MTox + batch 3 69 Ref : In vitro test, Utrecht University, NL, 2005
17
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19 CLAY ACTIVATION: THE P.I.L.C REVOLUTION Removal of the limiting factor Opening of the interlayer spaces allowing the entrance of the big size mycotoxins in the structure and their binding by adsorption on the internal surfaces. A wide spectrum of adsorption NO with standard clay YES with Nanotechnology MT.X+
20 Montmorillonite Yeast cell walls Diatomaceous earth + + =
21 Spectrum of Activity AFLATOXINS OCHRATOXINS CLAYS ZON DON T-2 FUMONISIN YEAST CELL WALL
22 TRIAL RESULTS Asia Effects of MT.X+ inclusion in the diet on health and performance of broilers in Vietnam
23 M TX+ AND LAYING HENS Malaysia Singapore months trial layers 2 sheds involved 1kg MT.X+ / ton ( = 40gr MT.X+/hen / year )
24 TRIAL RESULTS Asia Effects of MT.X+ on the performance of laying hens in Malaysia
25 MTX+ in Hatchery In hatchability percentage : we improved our hatchability up to 2% We save cull percentage by 1.5% where as standard 2.0%. Cumulative hatchery performance improved by 3.5%.
26 MTX+ Nano Results in Pakistan Higher Daily Gain Reduced Mortality
27 MTX+ Nano Results in Pakistan When Newcastle Disease outbreak in Lahore Flocks fed with MTX+ Nano were not affected
28 Results in Pakistan Just accomplished research at University of Faisalabad with Pr M Zargham Khan DOC sigificantly reduced Bursa of Fabricius-thymus Decrease in the weight of immunological organs IgG, IgA, IgM bearing cells in spin and bursa signifacantly lower WHEN MYCOTOXINS IN DIET
29 AWARD - Malaysia 2007
30 IN CONCLUSION Mycotoxins under control Economic losses
31 IN CONCLUSION Mycotoxins under control Economic losses
32 Thanks for your attention
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