DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS OF MULTIPLE SCLEROSIS

DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS OF MULTIPLE SCLEROSIS John R. Rinker, II, Anne H. Cross ABSTRACT Making the diagnosis of multiple sclerosis (MS) remains a challenging problem for clinicians. The foundation of diagnosis rests on demonstrating neurologic lesions, predominantly in the white matter, that are disseminated over space and time. Diagnostic criteria guide the clinician through applying clinical, radiologic, and laboratory evidence to secure the diagnosis. Complicating the process, however, is the existence of other conditions that may resemble MS and mislead the clinician to the wrong diagnosis. A familiarity with the differential diagnoses of symptoms commonly associated with MS permits the clinician to make the most accurate diagnosis. Continuum Lifelong Learning Neurol 2007;13(5):13 34. INTRODUCTION Multiple sclerosis (MS) begins in 85% to 90% of patients with relapsing and remitting neurologic symptoms localized to the brain, optic nerve, or spinal cord. Symptoms correlate with the formation of gliotic scars, or plaques, in the CNS, following attacks of inflammatory demyelination. No test, short of tissue biopsy, is 100% specific in securing the MS diagnosis. This problem has led to the publication and refinement over time of multiple sets of diagnostic criteria, all of which rest on two basic tenets: (1) CNS lesions should be disseminated in space and time, and (2) other potential explanations for the symptoms should be reasonably ruled out. This chapter discusses the most recently published diagnostic criteria for MS and the differential diagnoses when considering other potential causes of neurologic symptoms. DIAGNOSTIC CRITERIA The most recent set of diagnostic criteria for MS are the 2005 revisions to the McDonald criteria (Table 1-1) (Polman et al, 2005). The McDonald criteria allow for diagnosis based on clinical presentation alone if multiple attacks accompanied by clinical evidence of at least two lesions can be identified. The definition of an attack is generally accepted to be the development of neurologic symptoms likely caused by an inflammatory demyelinating lesion, lasting at least 24 hours and supported by objective findings. Since many patients early in the disease course may not meet the strict clinical definition for MS, the McDonald criteria allow for the use of ancillary testing such as MRI, CSF analysis, and visual evoked potentials (VEPs) (Gronseth and Ashman, 2000; Appendix A) to satisfy the requirements for dissemination in space and time. KEY POINTS: A The diagnosis of relapsing multiple sclerosis (MS) requires the demonstration of multiple discrete neurologic events, arising from lesions in the white matter of the CNS, disseminated over time, without an alternative explanation. A Published diagnostic criteria incorporate MRI, CSF, and evoked potential studies to expand a clinician s ability to demonstrate lesion dissemination in space and time. 13

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS TABLE 1-1 2005 Revisions to the McDonald Diagnostic Criteria for Multiple Sclerosis Clinical Presentation Two or more attacks; a objective clinical evidence of two or more lesions Two or more attacks; a objective clinical evidence of one lesion One attack; a objective clinical evidence of two or more lesions One attack; a objective clinical evidence of one lesion (monosymptomatic presentation; clinically isolated syndrome) Additional Data Needed for Multiple Sclerosis Diagnosis None b Dissemination in space, demonstrated by: MRI c or Two or more MRI-detected lesions consistent with MS plus positive CSF d or Await further clinical attack a implicating a different site Dissemination in time, demonstrated by: MRI e or Second clinical attack a Dissemination in space, demonstrated by: MRI c or Two or more MRI-detected lesions consistent with MS plus positive CSF d and Dissemination in time, demonstrated by: MRI e or Second clinical attack a 14 If criteria indicated are fulfilled and there is no better explanation for the clinical presentation, the diagnosis is MS; if suspicious, but the criteria are not completely met, the diagnosis is possible MS ; if another diagnosis arises during the evaluation that better explains the entire clinical presentation, then the diagnosis is not MS. a An attack is defined as an episode of neurologic disturbance for which causative lesions are likely to be inflammatory and demyelinating in nature. There should be subjective report (backed up by objective findings) or objective observation that the event lasts for at least 24 hours. b No additional tests are required; however, if tests (MRI, CSF) are undertaken and are negative, extreme cautionneedstobetakenbeforemakingadiagnosisofms.alternativediagnosesmustbeconsidered.theremust be no better explanation for the clinical picture and some objective evidence to support a diagnosis of MS. c MRIdemonstrationofspacedisseminationmustfulfillthecriteriaderivedfromBarkhofandcolleagues(1997) and Tintoré and colleagues (2000). d PositiveCSFdeterminedbyoligoclonalbandsdetectedbyestablishedmethods(isoelectricfocusing)different from any such bands in serum, or by an increased immunoglobulin G index. e MRI demonstration of time dissemination must fulfill the criteria. Barkhof F, Filippi M, Miller DH, et al. Comparison of MR imaging criteria at first presentation to predict conversion to clinically definite multiple sclerosis. Brain 1997;120(pt 11):2059 2069. Tintoré M, Rovira A, Martinez MJ, et al. Isolated demyelinating syndromes: comparison of different MR imaging criteria to predict conversion to clinically definite multiple sclerosis. AJNR Am J Neuroradiol 2000;21(4):702 706. From Polman CH, Reingold SC, Edan G, et al. Diagnostic criteria for multiple sclerosis: 2005 revisions to the McDonald Criteria. Ann Neurol 2005;58:840 846. Reprinted with permission from John Wiley & Sons, Inc. Copyright # 2005, American Neurological Association. In most early cases, establishing dissemination in space is more readily accomplished than dissemination in time. If clinical evidence of at least two lesions cannot be found on neurologic examination, MRI will often provide evidence of disseminated brain or spinal cord lesions. Specific MRI criteria exist for demonstrating dissemination in space (Table 1-2). CSF containing oligoclonal bands or elevated immunoglobulin (Ig) G index may also

contribute to the criteria for dissemination in space. This may be particularly useful when clinical history or MRI is not strongly suggestive of MS. TABLE 1-2 MRI Criteria in Multiple Sclerosis " Dissemination in Space Three of the following: 1. At least one gadoliniumenhancing lesion or nine T2 hyperintense lesions if there is no gadolinium-enhancing lesion 2. At least one infratentorial lesion 3. At least one juxtacortical lesion 4. At least three periventricular lesions NOTE: A spinal cord lesion can be considered equivalent to a brain infratentorial lesion: an enhancing spinal cord lesion is considered to be equivalent to an enhancing brain lesion, and individual spinal cord lesions can contribute together with individual brain lesions to reach the required number of T2 lesions. " Dissemination in Time 1. Detection of gadolinium enhancement at least 3 months after the onset of the initial clinical event, if not at the site corresponding to the initial event 2. Detection of a new T2 lesion if it appears at any time compared with a reference scan done at least 30 days after the onset of the initial clinical event Data from Barkhof F, Filippi M, Miller DH, et al. Comparison of MR imaging criteria at first presentation to predict conversion to clinically definite multiple sclerosis. Brain 1997;120(pt 11): 2059 2069. Data from Tintoré M, Rovira A, Martinez MJ, et al. Isolated demyelinating syndromes: comparison of different MR imaging criteria to predict conversion to clinically definite multiple sclerosis. AJNR Am J Neuroradiol 2000;21(4):702 706. An initial neurologic event suggestive of demyelination is commonly termed a clinically isolated syndrome (CIS). Patients who experience a CIS and have MRI or CSF evidence of dissemination in space are at high risk for experiencing additional demyelinating attacks. The interval until the second clinical attack is unpredictable. Some patients may relapse after a short time period, which satisfies the MS criterion for dissemination in time, while others may not relapse for months or years, and a small percentage may not relapse again. In the absence of a second clinical attack, MRIhasbecomeausefultoolindemonstrating ongoing lesion formation in patients suspected of having MS. MRI may thus act as a surrogate for a clinical relapse, and a diagnosis can be made ahead of a second clinical attack. By using MRI to demonstrate dissemination in time, patients with a CIS may benefit from early therapeutic interventions that delay the onset of a second clinical event. The MRI criteria for demonstrating dissemination in time (Table 1-2) are defined in the context of the revised McDonald criteria, similar to the criteria for dissemination in space. DIAGNOSTIC CRITERIA FOR PROGRESSIVE-ONSET DISEASE AminorityofpatientswithMS(10%to 15%) present with disease that is progressive from onset, so-called primary progressive MS (PPMS) or progressiverelapsing MS. Since these patients do not experience clear attacks like patients with relapsing-remitting onset, separate criteria have been developed to diagnose MS in patients with progressive onset (Table 1-3). The criteria are consistent with the relapsing-onset criteria in that dissemination in space must be demonstrated in the form of clinical findings, positive brain or spinal cord MRI, VEPs, or positive CSF. Dissemination in time is determined by progression of neurologic symptoms 15

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS 16 KEY POINT: A In progressiveonset MS, dissemination in time may be satisfied by documenting increasing symptoms or signs over a 1-year period. TABLE 1-3 Clinical Presentation Insidious neurologic progression suggestive of multiple sclerosis 2005 Revisions to the McDonald Criteria for Progressive-onset Multiple Sclerosis Diagnostic Criteria One year of disease progression (retrospectively or prospectively determined) and Two of the following: Positive brain MRI (nine T2 lesions or four or more T2 lesions with positive visual evoked potentials) a Positive spinal cord MRI (two focal T2 lesions) Positive CSF b a Abnormal visual evoked potentials of the type seen in multiple sclerosis. b Positive CSF determined by oligoclonal bands detected by established methods (isoelectric focusing) different from any such bands in serum, or by an increased immunoglobulin G index. From Polman CH, Reingold SC, Edan G, et al. Diagnostic criteria for multiple sclerosis: 2005 revisions to the McDonald Criteria. Ann Neurol 2005;58:840 846. Reprinted with permission from John Wiley & Sons, Inc. Copyright # 2005, American Neurological Association. syndromes, abnormal MRI scans, and inflammatory CSF. MS is a disease with heterogeneous symptoms, course, disability, and laboratory findings. An overly broad approach to generating a differential diagnosis is often of low yield; instead, the differential should arise from the specific symptoms, imaging, and laboratory abnormalities of each patient. The sections below discuss diagnostic considerations of frequently encountered symptoms and TABLE 1-4 Differential Diagnosis of Suspected Optic Neuritis " Multiple Sclerosis " Sarcoidosis " Neuromyelitis Optica (Devic s Disease) " Recurrent Idiopathic Optic Neuritis " Rheumatologic Disease Systemic lupus erythematosus Sjögren syndrome Antiphospholipid antibody syndrome Behçet s disease " Infection West Nile virus HIV Varicella-zoster virus over a 1-year time period, either prospectively or retrospectively. DIFFERENTIAL DIAGNOSIS A prerequisite to applying the diagnostic criteria described above is the exclusion of other diagnoses that might better explain a patient s symptoms and laboratory findings. The differential diagnosis of MS can lead to a broad discussion of clinical Cryptococcus Toxoplasmosis Syphilis Histoplasmosis " B 12 Deficiency " Retinal Artery Occlusion " Retinal Detachment " Acute Glaucoma

circumstances associated with a possible diagnosis of MS. CLINICAL SYNDROMES Vision Loss Numerous inflammatory and noninflammatory conditions may present in a manner similar to optic neuritis (Table 1-4). Optic neuritis typically presents with the acute to subacute onset of unilateral or bilateral vision loss, often associated with pain. Color discrimination, contrast sensitivity, and visual acuity may all be affected in the attack. Recovery is variable and inversely relates to the severity of the attack. A course of IV steroids may hasten the recovery process but does not alter the long-term prognosis (Brusaferri and Candelise, 2000). The pain accompanying optic neuritis is typically retrobulbar and often exacerbated by bright light or eye movement. If the history and bedside examination do not sufficiently narrow the differential diagnosis, referral to an ophthalmologist is warranted. Neuromyelitis optica ([NMO] Devic s disease), a disease similar to, but distinct from, MS, limits itself in most cases to the optic nerves and spinal cord (Case 1-1). Acute bilateral optic neuritis should suggest the diagnosis of NMO. The vision loss in NMO also tends to be more severe, and recovery is less complete than in many cases of MS-associated optic neuritis (Pirko et al, 2004). Systemic autoimmune inflammatory diseases such as systemic lupus erythematosus (SLE), antiphospholipid antibody syndrome, Sjögren syndrome, and Behçet s disease can also cause optic neuropathies that may be clinically Case 1-1 A pregnant 38-year-old woman awakens one morning with graying out of vision in her right eye. By day s end she has no light perception in the eye. She is not treated because of her pregnancy, and she recovers to normal visual acuity after 3 weeks. Two years later she has a recurrence of vision loss in the right eye, but this time accompanied by heaviness in her right leg. She is given oral prednisone and improves back to baseline within 3 weeks. At age 45, the patient develops simultaneous bilateral vision loss over 2 days. IV steroid treatment leads to incomplete recovery with persistent visual defects. At age 55, the patient develops numbness over several days, first the right leg, then the left leg, that soon evolves into weakness and eventually complete paraplegia. Urinary retention also develops. Brain MRI is normal, but spinal cord MRI reveals a continuous T2 cord lesion extending from T1 to T8. CSF analysis reveals a white blood cell count of 13, a protein of 58, an elevated IgG index of 0.75, and no oligoclonal bands. IV steroids again result in improvement of the symptoms. Comment. Because of the occurrence of optic neuritis and transverse myelitis in both MS and NMO (Devic s disease), the two conditions were long thought to represent variations of the same disease. Neuropathologic differences as well as the recent identification of an autoantibody to the aquaporin-4 water channel (NMO-IgG) in patients with NMO suggest NMO is pathologically distinct from MS. Distinguishing features of NMO include spinal cord lesions extending over at least three segments, absence of oligoclonal bands in the CSF, a strong association with other autoimmune diseases, and paucity or absence of brain lesions on MRI. The NMO-IgG antibody was present at a 1:3200 titer in the case described. 17

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS 18 KEY POINTS: A While most patients with optic neuritis and an abnormal MRI go on to develop clinically definite MS, conditions such as neuromyelitis optica, rheumatologic disorders, sarcoidosis, B 12 deficiency, and various viral infections may also cause optic neuropathies. A Spinal cord disease in MS may develop acutely as part of a relapse or gradually as part of a progressive course; the manner of presentation should shape the differential diagnosis when considering alternative causes. indistinguishable from MS-associated optic neuritis (Theodoridou and Settas, 2006). Systemic sarcoidosis may also associate with neurologic syndromes in 5% to 26% of affected patients, and optic neuropathy belongs to the collection of symptoms associated with this condition (Stern, 2004). Most CNS manifestations of sarcoidosis manifest as isolated cranial neuropathies or in combination with meningeal symptoms. Thus,inthesettingofsarcoid-related optic neuropathy, gadolinium-enhanced MRI will often reveal enhancement of theopticnervesheath(case 1-2). Infection also belongs in the differential diagnosis of optic neuritis-like syndromes. West Nile encephalomyelitis is reported to cause an infectious optic neuritis (Chan et al, 2006), and HIV has been associated with optic neuritis alone and in association with varicella-zoster, Cryptococcus, and toxoplasmosis (Jabs, 1995). A substantial proportion of patients presenting with an initial episode of optic neuritis will not develop clinically definite MS. In a 10-year followup study of patients with optic neuritis, 38% went on to develop MS. The presence of at least one lesion on brain MRI was associated with a 56% chance of developing clinically definite MS within the next 10 years, while patients with a normal MRI had only a 22% chance of developing clinically definite MS (Beck et al, 2003). Myelopathy Spinal cord dysfunction in MS may occur in association with relapses, most often as partial transverse myelitis, or may develop as a slowly progressive myelopathy in PPMS or secondary progressive MS (Table 1-5). Because of the differing presentations, the differential for spinal cord disease in MS must be broad enough to include inflammatory, vascular, and toxic/degenerative conditions. The clinical presentation of the patient and Case 1-2 A 46-year-old woman develops vision loss in the right eye over 3 days. Her visual acuity progresses to no light perception and never recovers. Several weeks later, she begins to lose vision in the left eye. MRI of the brain and optic nerves reveals no evidence of white matter lesions, but gadolinium enhancement is noted in the sheaths around both optic nerves. IV steroids result in stabilization of vision in the left eye but no improvement in the right eye. CSF is normal, as are antinuclear antibody and extractable nuclear antigens. Serum and CSF angiotensin-converting enzyme levels are also normal, and there is no evidence of granulomatous disease on chest CT. Two months after her discharge from the hospital, subcutaneous nodules develop in the patient s right arm. Biopsy of these reveals noncaseating granulomas, and she is started on maintenance prednisone for systemic sarcoidosis and remains relapse free. Comment. Sarcoidosis affects the CNS in 5% of cases. Fifty percent of patients with neurosarcoidosis present with neurologic symptoms, but only rarely does sarcoidosis limit itself to the CNS. When sarcoidosis does affect the CNS, cranial neuropathies are the most common manifestation. Meningitis, hydrocephalus, endocrinopathies, and mass lesions may all occur as well. Biopsy of suspicious pulmonary or other lesions yields a definitive diagnosis. Results of less-invasive tests, such as elevated levels of serum and CSF angiotensin-converting enzyme, are insensitive and not pathognomonic for the disease.

TABLE 1-5 Differential Diagnosis of Nontraumatic Myelopathy " Acute Multiple sclerosis Neuromyelitis optica (Devic s disease) Idiopathic transverse myelitis Epidural abscess Spinal arteriovenous malformation Mycoplasma Rheumatologic disease Spinal epidural hematoma " Progressive Multiple sclerosis Cervical stenosis Syrinx Human T-lymphotropic virus type 1 HIV Copper deficiency B 12 deficiency Spinal arteriovenous malformation Hereditary spastic paraparesis Adrenomyeloneuropathy Epidural tumor Dural venous fistula the appearance of MRI lesions help the clinician direct the diagnostic approach to either an acute or progressive myelopathy. MS-associated transverse myelitis typically presents with asymmetric sensory and motor deficits. Symptoms evolve over hours to days and may be accompanied by bladder and bowel dysfunction. Symmetric onset is less characteristic of MS and more characteristic of acute transverse myelitis, either as an isolated event or as part of a syndrome such as NMO (Case 1-1). MS spinal cord lesions are longitudinally short on MRI. Multiple MS lesions may give the cord a mottled appearance. In contrast, spinal cord lesions in NMO are longitudinally extensive, often traversing three or more spinal levels and sometimes even extending into the inferior brain stem (Wingerchuk et al, 2006). Disability prognosis is also worse among individuals with longitudinally extensive transverse myelitis, with greater severity at onset and worse overall recovery from relapses. A wide range of immune-mediated conditions may cause transverse myelitis. Medical history may give some clues to the etiology, such as an antecedent illness in a postinfectious myelitis, or other systemic autoimmune phenomena, such as photosensitive rash or arthralgias in rheumatologic disease. Sometimes, even when an infectious or autoimmune etiology is ultimately found, the initial history and general examination may not yield clues to the underlying cause. Screening for autoantibodies may yield results directing the clinician to a diagnosis such as systemic sclerosis (Torabi et al, 2004) or antiphospholipid antibody syndrome (rarely) and Sjögren syndrome or SLE (more commonly) (Theodoridou and Settas, 2006). All of these conditions may present with neurologic symptoms before the development of their more commonly recognized phenotypes. Infection may cause both acute and progressive myelopathies. Retroviruses such as HIV and human T-lymphotropic virus type 1 (HTLV-1) tend to present with slowly progressive spinal cord syndromes. Motor, sensory, and sphincter function can all become impaired. HIV-associated myelopathy most commonly results KEY POINTS: A Distinguishing characteristics of idiopathic or neuromyelitis optica associated transverse myelitis include symmetric symptoms at onset and longitudinally extensive cord lesions at least three or more segments long on MRI. A The yield of finding relevant autoantibodies in patients with acute myelitis is highest when symptoms of other autoimmune conditions are present. In rare cases, diseases such as systemic lupus erythematosus, Sjögren syndrome, and antiphospholipid antibody syndrome may present with an inflammatory myelitis. 19

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS 20 KEY POINTS: A Retroviral infection by HIV or human T-lymphotropic virus type 1 produces a slowly progressive myelopathy, while pathogens such as varicellazoster virus, cytomegalovirus, or Mycoplasma pneumoniae cause an acutely symptomatic myelitis. A Numerous noninflammatory conditions may produce a progressive myelopathy resembling that seen in primary progressive MS. Examples include nutritional deficiencies, cervical stenosis, arteriovenous malformations, and syrinx. directly from HIV in patients with low CD4 counts (McArthur et al, 2005); however, patients who are CD4 deficient are also susceptible to opportunistic infection by other potentially myelopathic organisms, such as cytomegalovirus, Epstein-Barr virus, herpes simplex virus, Treponema pallidum (syphilis), and Mycobacterium tuberculosis. Mycoplasma pneumoniae is an intracellular bacterium that preferentially infects the upper or lower respiratory tracts and that has been associated with a variety of CNS complications, including transverse myelitis. Typical presentation includes a rapidly progressive loss of motor function followed by loss of bladder or bowel function and sensory changes. Theories of pathogenesis include direct infection of the affected tissue, hypercoagulability causing vascular occlusion, and molecular mimicry leading to parainfectious immune-mediated injury. Diagnosing Mycoplasma-related myelitis requires reasonable evidence that the patient has been infected with M. pneumoniae (Guleria et al, 2005; Tsiodras et al, 2005). Patients with spinal cord arteriovenous malformations may occasionally present with relapsing myelopathic symptoms. Paralysis, numbness, and pain are all frequently associated with this condition. Left untreated, many patients develop severe gait impairment. Spinal cord arteriovenous malformations are most common among middle-aged men, making them an important consideration in the differential diagnosis of PPMS (which also frequently presents as a progressive myelopathy in middle-aged men). Diagnosing spinal cord arteriovenous malformations may be difficult. CSF immune studies should be normal. They may not be appreciated on routine MRI, and spinal cord angiography may be required to definitively diagnose and ultimately treat the condition. Structural abnormalities of the spinal cord or spinal column may also result in progressive myelopathies. Cervical spondylosis causing spinal canal stenosis and subsequent cord compression may cause progressive motor, sensory, sphincter, or sexual disturbances. Cervical stenosis often, but not always, causes neck pain, which may radiate into the extremities. MRI and CT myelogram are the most useful tests in diagnosing this condition. Syringomyelia (syrinx) may also cause progressive myelopathy due to expansion of the spinal cord central canal and has a characteristic clinical presentation featuring hand and arm weakness and cape-distribution sensory loss. A syrinx is most often identified by sagittal MRI scan showing expansion of a fluid-filled space. Vitamin B 12 and copper deficiency are both associated with progressive myelopathies. In B 12 deficiency, the myelopathy occurs as part of a condition called subacute combined degeneration (Case 1-3). Subacute combined degeneration begins with impaired vibration sense and proprioception, modalities carried by the posterior columns. Motor dysfunction often follows. T2 hyperintensities may be identified in both spinal cord and brain and can resemble MS lesions. It is important to note that symptoms and signs of B 12 deficiency may occur even with B 12 levels within the normal range and in the absence of macrocytic anemia. Low or borderline B 12 values should be followed up with serum methylmalonic acid and homocysteine levels, which are elevated in true B 12 -deficient states (Allen et al, 1990). A finding of elevation of methylmalonic acid is more specific to B 12 deficiency, however. Insufficient levels of copper may also lead to progressive myelopathy, which may be confused with progressive-onset MS. As with B 12 deficiency, gastric bypass or other intestinal surgeries

Case 1-3 A 44-year-old woman develops a left-sided footdrop. After 3 to 4 months, the footdrop improves but does not resolve. In subsequent years, she experiences episodes of diminished visual acuity in first the right, then the left eye. In both cases, the vision recovers after a period of weeks to months. Five to 6 years after her initial symptoms, she begins to notice a persistent clumsiness in her walking, and she notices a decreased ability to discriminate temperature in her feet. Examination reveals decreased visual acuity in the left eye (20/70). Disc pallor is also noted on the left. The only weakness appreciated is a left footdrop. Temperature and vibratory sensation are severely decreased in the bilateral toes. Reflexes are normal, and gait is abnormal due to her footdrop. Brain and C-spine MRI are both normal. CSF evaluation is also normal. VEPs confirm a P100 conduction delay in the left optic pathway. The serum B 12 level is 263 pg/ml on initial check and 188 pg/ml on recheck 2 years later. Subsequently, methylmalonic acid levels are found to be markedly elevated at 1227 nmol/l. The patient is found to be mildly anemic with a normal mean corpuscular volume. Serum antibodies against intrinsic factor are detected, and B 12 replacement therapy eventually leads to correction of the B 12 and methylmalonic acid abnormalities. Comment. Subacute combined degeneration specifically describes the spinal cord pathology associated with vitamin B 12 deficiency, but peripheral neuropathy, optic neuropathy, and neuropsychiatric symptoms may all occur as well. The low normal B 12 level identified early on in this patient falls within the range of values (less than 300 pg/ml) associated with elevated methylmalonic acid and homocysteine in 5% to 10% of cases. Such a finding warrants beginning B 12 replacement therapy. have been associated with acquired copper deficiency. Excessive zinc intake has also been associated with copper deficiency (Kumar et al, 2004). For both conditions, early diagnosis is important, as replacement may lead to a cessation of disease progression and possible reversal of signs and symptoms. Lastly, one should consider the several inherited conditions that may cause progressive myelopathy. Hereditary spastic paraparesis causes, as its name suggests, progressive spastic weakness in the lower extremities. In its pure form, sensory and sphincter functions are generally preserved. Inheritance is usually autosomal dominant (McDermott et al, 2000). Adrenomyeloneuropathy is an X-linked disorder that manifests primarily as a slowly progressive myelopathy, with or without clinical involvement of peripheral nerves (Case 1-4). MRI in adrenomyeloneuropathy may look similar to MS. The spinocerebellar ataxias may also feature prominent symptoms of spinal cord dysfunction. Clues to distinguishing hereditary myelopathies from MS include a strong family history, lack of typical T2-weighted MRI findings, and normal CSF studies. Genetic tests are now commercially available. BRAIN STEM SYNDROMES While MS lesions may develop in any myelinated areas of the brain stem, certain symptoms and syndromes are more typical of MS than others. A familiarity with alternative etiologies for ataxia, internuclear ophthalmoplegia (INO), oculomotor dysfunction, trigeminal neuralgia, and facial nerve palsy 21

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS Case 1-4 Over a 2-year period, a 44-year-old man develops balance difficulty while walking and running. The symptoms begin insidiously and progress gradually. Bladder and bowel function have been normal, but sexual function has diminished. Family history is remarkable for a nephew who at age 7 developed a degenerative neurologic condition characterized by loss of language function, seizures, dementia, and spastic quadriplegia. The nephew tested positive for elevated levels of very long chain fatty acids (VLCFAs). Subsequent testing in asymptomatic family members revealed abnormal VLCFA levels in the patient s mother and a maternal aunt. The patient himself has a dark complexion. He also has orthostatic hypotension, with blood pressure dropping from 130/80 mm Hg to 100/65 mm Hg within 1 minute of standing. The patient s neurologic evaluation reveals normal cognitive function. He has a spastic paraparesis with lower extremity hyperreflexia. Sensation is decreased to vibration, but light touch, pinprick, and proprioception are normal. Brain MRI reveals no white matter abnormalities. VLCFA testing on the patient reveals higher than normal levels, consistent with a defect in peroxisomal fatty acid oxidation. The patient is diagnosed with adrenomyeloneuropathy. Comment. While childhood-onset adrenoleukodystrophy constitutes the most common presentation of this X-linked disease, a substantial minority of patients present with a clinical variant known as adrenomyeloneuropathy. Mild cognitive dysfunction occurs in about half of patients with adrenomyeloneuropathy, and brain MRI abnormalities are occasionally seen. The reason adrenomyeloneuropathy is most likely to be confused with MS arises from a progressive myelopathy, which may resemble PPMS. 22 allows the clinician to identify conditions that may resemble MS (Table 1-6). Infectious, postinfectious, and postvaccinal acute disseminated encephalomyelitis (ADEM) may all cause demyelinating lesions affecting the cerebellum or other parts of the brain stem. ADEM does not typically confine itself to the infratentorial CNS, but it may, especially following varicella-zoster virus (Menge et al, 2005). Epstein- Barr virus and Mycoplasma have also been associated with ataxia syndromes (Nussinovitch et al, 2003). A history of antecedent illness or recent vaccination gives some clue to a possible infectious or postinfectious process. Brain MRI may be dramatic with extensive, confluent cerebral and cerebellar disease. Recovering patients may experience substantial improvement in their lesion load. CSF may have an inflammatory appearance similar to that seen in MS, but unlike MS this typically normalizes with time. Infection with Borrelia burgdorferi, the organism that causes Lyme disease, most often begins with a rash at the site of infection. A subset of patients develop neurologic problems, which often involve cranial nerves. The facial nerve in particular may be affected by disseminated Lyme disease. Lyme disease is prevalent in a relatively limited number of geographic areas in the United States. Knowledge of the local prevalence rate and the travel history of the patient should help dictate whether serum antibodies to B. burgdorferi should be measured.

TABLE 1-6 " Ataxia Multiple sclerosis Differential Diagnosis of Progressive Ataxia and Brain Stem Syndromes Spinocerebellar ataxias Wernicke syndrome Vitamin E deficiency Viral or postinfectious encephalitis " Internuclear Ophthalmoplegia Multiple sclerosis Cerebrovascular disease Cerebral autosomal dominant arteriopathy with subcortical infracts and leukoencephalopathy Myasthenia gravis (pseudo-internuclear ophthalmoplegia) " Trigeminal Neuralgia Idiopathic Brain stem tumor Multiple sclerosis When sarcoidosis involves the nervous system, cranial nerves are commonly involved (50% to 75% cases). Twenty-five percent to 50% may have facial nerve palsy. Other common signs of neurosarcoidosis include aseptic meningitis, brain lesions, seizures, hypothalamic and endocrine dysfunction, and peripheral neuropathy (Stern, 2004). Sarcoidosis may present initially with nervous system involvement; the more commonly recognized pulmonary disease may not be evident. Cerebrovascular disease commonly causes INO, one of the more distinct syndromes associated with MS (Keane, 2005) (Case 1-5). Clues to a cerebrovascular etiology of a new INO include a medical history with risk factors for stroke, abrupt onset, a diffusionrestricted lesion on MRI, and an absence of MS-typical brain or CSF abnormalities. Ataxia or ophthalmoplegia in someone with a history of nutritional deficiencies, frequent vomiting, or heavy alcohol consumption, with or without abnormalities on brain MRI, should make Wernicke encephalopathy a consideration. Another condition seen in alcoholics (particularly in the setting of malnourishment) and also in patients who have had rapid correction of significant hyponatremia (less than 130 meq/l) is central pontine myelinolysis. Fulminant central pontine myelinolysis may present with rapidly progressive quadriplegia and inability to chew, speak, or swallow and can leave the patient with lockedin syndrome or result in death. Less severe presentations may also occur. The myelinolysis appears as T2 hyperintensity on brain MRI and may involve extrapontine regions as well. Vitamin E deficiency, although rare, is another condition that may cause progressive ataxia, commonly accompanied by a peripheral neuropathy. Gastrointestinal conditions leading to fat malabsorption generally or vitamin E specifically may result in symptomatic vitamin E deficiency. The inherited spinocerebellar ataxias may also be confused clinically with MS, especially in cases lacking a clear history. A slowly progressive course, lack of MRI or CSF abnormalities, and positive genetic testing clarify the diagnosis. PAIN SYNDROMES Pain and other uncomfortable sensory syndromes may constitute the initial symptom in MS (Table 1-7). Due to the prevalence of neuropathic and idiopathic pain syndromes in the general population, distinguishing MSrelated pain from other conditions may present a challenge. KEY POINTS: A Although internuclear ophthalmoplegia is commonly associated with MS, cerebrovascular disease is a more common cause and should be considered in the absence of other symptoms suggestive of MS. A Nutritional deficiencies (eg, thiamine, vitamin E) and rapid correction of electrolyte disturbances (eg, hyper-, hyponatremia) may result in brain stem signs including ataxia and oculomotor palsies. 23

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS Case 1-5 A 39-year-old woman without risk factors for cerebrovascular disease acutely develops dysequilibrium and diplopia when looking to the right. One day later her left arm develops paresthesias, and over the course of a day she develops similar sensations in both legs and her gait becomes unsteady. After taking a day off from work, her symptoms begin to resolve and by 3 weeks are completely gone. An ophthalmologist had evaluated the patient on the first day of symptoms and diagnosed her with an INO. MRI of the brain reveals a solitary T2 lesion in the right thalamus, and magnetic resonance angiography shows normal posterior circulation vasculature. Lumbar puncture is performed, revealing elevated IgG index, synthesis rate, and positive oligoclonal bands. When MRI is repeated 3 months later, new T2 lesions have formed in the subcortical white matter, meeting the McDonald criteria for relapsing MS. Comment. Although INO is commonly associated with MS, cerebral infarction accounts for more cases of INO than MS. In addition, while MS lesions are generally identified on MRI in the CNS white matter, gray matter lesions can and do occur. In cases such as this one, the patient s age and lack of risk factors for stroke raised the concern for inflammatory demyelinating disease. CSF was ultimately helpful in diagnosing the patient s event as a clinically isolated demyelinating syndrome. The subsequent diagnostic and therapeutic approaches were guided by this result. 24 Certain pain syndromes are more commonly experienced by patients with MS than others. Trigeminal neuralgia occurs uncommonly in MS (1.9% in one series) and may be clinically indistinguishable from idiopathic trigeminal pain. Clues to a possible demyelinating etiology include younger age of onset (less than 40 years) and bilateral symptoms (Zakrzewska, 2002). While trigeminal pain may be the heralding symptom of MS, in most cases it develops later in the disease course. TABLE 1-7 Pain Syndromes Associated With Multiple Sclerosis " Pseudoradicular pain " Lhermitte sign " Paroxysmal spasms " Trigeminal neuralgia " Migraine More commonly, trigeminal neuralgia arises as the consequence of a posterior fossa tumor (10% of cases) or as an isolated condition (85% of cases). Lhermitte sign, or the sensation of radiating electrical sensation elicited by neck flexion, can be painful or disconcerting to patients. Despite the common association of Lhermitte sign with MS, it is not specific and is uncommonly present at diagnosis (5% to 10%) (Al-Araji and Oger, 2005). Lhermitte sign may be caused by multiple conditions affecting the cervical spinal cord. Cervical stenosis with or without cord compression, extrinsic and intrinsic spinal tumors, spinal cavernous angioma, and B 12 deficiency have all been associated with Lhermitte sign. Tonic spasms are paroxysmal attacks of involuntary muscle contraction that frequently migrate into adjacent parts of the body and may cause intense discomfort. They seldom constitute the initial presentation of demyelinating disease, but do occur

in 2% to 20% of patients with MS over the disease course (Solaro et al, 2004). Other demyelinating diseases, such as NMO, and cerebral infarctions involving the corticospinal tracts have also been associated with tonic spasms. Migraine headaches may occur in the MS population more commonly than in the general population (LaMantia and Erbetta, 2004), but only limited evidence suggests MS may present as a migraine disorder. More commonly, migraineurs will have a brain MRI performed because of headaches, and white matter hyperintensities on T2- weighted scans will be identified. In this setting, it is important for the clinician to be aware of possible occult conditions that may be associated with both headache and white matter brain abnormalities. Neurosarcoidosis, Behçet s syndrome, and ADEM are all inflammatory conditions that may give rise to such a combination of symptoms and imaging abnormalities. Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) often begins with migraine headaches. Brain masses and infection may be associated with T2 hyperintensities and headaches, although focal neurologic signs or systemic symptoms (such as fever) are most often present. The most common types of pain experienced by patients with MS over the course of their disease are dysesthetic limb and back pain. While reports of patients presenting with such symptoms exist in the literature (Marchettini et al, 2006), these are uncommon presentations. Since these pains are common in the general population, distinguishing instances of isolated dysesthetic pain due to MS from the more common conditions that cause such symptoms begins with a thorough history, examination, and appropriately directed ancillary studies (MRI, nerve conduction, EMG). PSYCHIATRIC AND COGNITIVE PRESENTATIONS In rare instances MS may present with exclusively cognitive or neuropsychiatric symptoms. Depression and progressive amnesia appear to be the most common features of such a presentation, but less common symptoms such as mania, aphasia, and overall personality changes may occur. One case series describes both relapsing-remitting and primary progressive courses following such an onset (Zarei et al, 2003). Because such presentations are rare, alternative diagnoses should be considered in these cases (Table 1-8). ADEM may present with chiefly neuropsychiatric symptoms, and brain MRI and CSF may also resemble MS (Case 1-6). Clues to a diagnosis of ADEM include vaccination or febrile illness prior to the onset of neurologic TABLE 1-8 Differential Diagnosis of Cognitive and Behavioral Syndromes With Abnormal MRI " Metachromatic leukodystrophy " Adrenoleukodystrophy (adult form) " Viral encephalitis (herpes simplex virus, others) " Postinfectious encephalitis " Multiple sclerosis " HIV " Lupus cerebritis " Progressive multifocal leukoencephalopathy " Cerebral autosomal dominant arteriopathy with subcortical infracts and leukoencephalopathy KEY POINTS: A A limited number of pain syndromes are sufficiently associated with MS to warrant a broader workup. These include trigeminal neuralgia in a young person, Lhermitte sign, and paroxysmal spasms. A Although migraine headaches are common in MS, the presence of migraine alone does not justify an extensive workup for demyelinating disease. 25

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS KEY POINT: A Purely cognitive or neuropsychiatric presentations of MS are quite rare. Encephalitis, acute disseminated encephalomyelitis, and metabolic conditions such as leukodystrophies should always be considered in the differential diagnosis. Case 1-6 A 24-year-old woman with a several-year history of stable bipolar disease develops mood swings and paranoia. Over 2 weeks, she is arrested 3 times for driving while impaired and is jailed for 3 months. While in jail, she develops marked short-term memory problems. She cannot hold a conversation because of her inattention. Upon returning to her family, she becomes repeatedly lost in familiar environments and shows violent tendencies. She is then brought to the emergency department. No focal neurologic deficits are identified on examination except for poor attention, poor concentration, and short-term memory loss. A brain MRI reveals multiple T2 and gadolinium-enhancing lesions in the supratentorial white matter. CSF reveals 34 nucleated cells (94% lymphocytes), IgG index of 4.44, IgG synthesis rate of 60.9, and 15 oligoclonal bands. No evidence of viral DNA is found in the CSF. Prolonged inpatient psychiatric management, including electroconvulsive therapy, results in eventual improvement in her symptoms. Follow-up MRI 2 months later shows resolution of the gadolinium enhancement, and repeat lumbar puncture reveals a downward trend in IgG levels (IgG index 2.17, IgG synthesis 34.6) and a stable number of oligoclonal bands. She continues to improve clinically and is eventually able to return to work. Comment. Although there are no upper limits to IgG index and synthesis rates in MS, levels well above normal should raise concerns about alternative diagnoses. IgG levels also tend to remain stable over time in MS. In addition, exclusively neuropsychiatric presentations of MS are quite uncommon. Although the exact diagnosis was never identified in this patient, an unidentified viral encephalitis or a postinfectious inflammatory process (such as ADEM) seems the most likely explanation. 26 symptoms and eventual resolution of both MRI and CSF abnormalities over time. Viral encephalitis may also present with delirium with or without focal neurologic deficits. Herpes simplex encephalitis in particular, with its predisposition for the temporal lobes, may cause personality changes and language impairment. T2 lesions in the temporal lobes and CSF positive for herpes simplex virus confirm the diagnosis. Although most cases of adrenoleukodystrophy and metachromatic leukodystrophy present during childhood or infancy, adult variants exist for both diseases, and neuropsychiatric symptoms may be the initial features of both (Natowicz and Bejani, 1994). Each condition may follow a progressive, rather than relapsingremitting, course, although years may pass before spasticity, visual loss, or other focal neurologic deficits occur. Both conditions feature white matter hyperintensities on T2-weighted MRI. Elevated CSF IgG has been reported in some cases of adrenoleukodystrophy. Specific enzymatic abnormalities distinguish both diseases: elevated levels of VLCFAs are found in adrenoleukodystrophy, and decreased arylsulfatase A is found in metachromatic leukodystrophy. A commercially available test that identifies mutations in the ABCD1 gene now exists for diagnosis of adrenoleukodystrophy. Abnormal MRIs According to a study from a universitybased MS center, approximately one

third of new diagnostic referrals concerned an abnormal MRI scan. Of these, only 11% of patients ultimately turned out to have MS, and all of those subjects provided history that was probably or possibly consistent with MS (Carmosino et al, 2005) (Case 1-7). While these referral figures describe the experience of a single specialty MS center, personal experience suggests the referral patterns described in this study are typical. This means that discovering presymptomatic MS based on an abnormal MRI is highly unlikely. A familiarity with the more common lesion characteristics associated with MS (Table 1-9) and the differential diagnosis of abnormal MRI is important to clinicians accepting referrals for possible MS. It is important to be aware of the frequent diagnoses associated with white matter hyperintensities on T2- weighted MRI. In the above-mentioned study, migraine or other headache disorder was the primary diagnosis given to patients referred because of abnormal MRI, accounting for 45% of cases. Age-related changes and hypertension accounted for the MRI abnormalities in 22% of patients. In an additional 22% of cases, no identifiable cause for the abnormalities was found. Together, these results illustrate the lack of specificity of MRI for MS and emphasize the importance of obtaining a history compatible with CNS inflammatory demyelination before assigning the diagnosis. Several diseases that may be confused with MS deserve special mention due to their prominent MRI abnormalities. Isolated granulomatous angiitis of the CNS causes focal neurologic KEY POINT: A The isolated discovery of white matter lesions on T2-weighted MRI seldom results in a diagnosis of MS; migraine and small vessel disease are common etiologies for nonspecific white matter disease in patients without a history of MS-typical symptoms. Case 1-7 A 41-year-old man with no past medical history is referred to the MS clinic because of an abnormal brain MRI. The scan had been obtained as part of a preoperative workup of headaches that were thought by the patient s otolaryngologist to be related to sinus disease. Careful history reveals no other past or current focal neurologic symptoms. The patient does have a 6-year history of increased fatigue, which has begun to limit his activity, subjective trouble with multitasking and concentration, and deceased libido. Neurologic examination reveals no abnormalities on the initial evaluation. T2-weighted brain MRI contains approximately 20 periventricular and juxtacortical white matter lesions, oriented perpendicularly to the lateral ventricles. No enhancement or infratentorial lesions are appreciated. CSF analysis reveals elevated IgG index and positive oligoclonal bands. On repeat evaluation 3 months after the initial consultation, the patient is found to have developed a partial right INO. Repeat MRI also reveals new T2 white matter lesions, and the patient is diagnosed with MS. Comment. Approximately half of all patients presenting with CIS have evidence of past demyelinating activity on MRI at presentation. Likewise, autopsy series have found evidence of MS in individuals who were asymptomatic during life. These observations suggest MS may begin as a subclinical disease, or even remain so over a patient s lifetime. While no guidelines exist to screen asymptomatic patients or patients with only vague, nonspecific symptoms with MRI, the discovery of imaging abnormalities consistent with MS deserve close follow-up should more typical MS symptoms develop over time. 27

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS KEY POINT: A The immunologic abnormalities measured in CSF reflect activation of the humoral immune response and are not specific to MS. TABLE 1-9 Patterns of MRI Abnormality in Multiple Sclerosis " Typical Multiple white matter lesions Lesions larger than punctate (>3 mm) Corpus callosum involvement Perpendicular orientation to ventricles (Dawson s fingers) Ovoid shape Gadolinium enhancement, sometimes ring enhancing Short (<3 segment) spinal cord lesions " Atypical (Abnormalities Seen in Multiple Sclerosis Infrequently) Tumorlike mass lesions Exclusively punctate lesions Anterior temporal lobe involvement Gray matter lesions (cortex, thalamus) Longitudinally extensive spinal cord lesions (3 segments) Sparing of corpus callosum Diffusion restriction Cognitive problems are common. The clinical picture may closely resemble MS. Lack of a family history should not be used to rule out this diagnosis, as de novo mutations of the NOTCH3 gene leading to CADASIL are not uncommon. The pattern of MRI lesions may offer a clue to the underlying diagnosis: CADASIL-associated lesions most frequently appear in the frontal, parietal, and anterior temporal cortex, and the external capsule (Singhal et al, 2005). Progressive multifocal leukoencephalopathy (PML) occurs chiefly in patients with advanced AIDS and most often presents with progressive hemiparesis, cortical visual loss, and mental status changes culminating in dementia, coma, and death over several months. PML also occurs following exposure to immunosuppressive agents; this diagnosis should be considered whenever an immunocompromised state is identified. The MRI abnormalities in PML occur in the white matter and most often are nonenhancing without mass effect. Followed over time, the lesions grow steadily in size, eventually becoming confluent (Bartt, 2006). Detecting JC virus DNA in the CSF confirms the diagnosis. 28 symptoms due to ischemic brain injury resulting from inflammation within CNS blood vessels (Case 1-8). These focal deficits are usually accompanied by headache and mental status changes. T2-weighted MRI typically reveals multifocal lesions, which may be confluent, affecting both gray and white matter. CSF pleocytosis and increased protein are also common (Younger, 2004). CADASIL usually presents with migrainelike headaches or strokelike focal neurologic deficits (Case 1-9). ABNORMAL CSF The CSF abnormalities most specific to MS are the presence of CSF-specific oligoclonal bands and elevation of the IgG index. Both tests measure abnormal immunoglobulin synthesis confined to the CNS, but neither test specifically links the humoral immune response to the pathogenic process. This becomes problematic when considering inflammatory conditions other than MS that may affect the CNS (Table 1-10). Sarcoidosis, SLE, antiphospholipid antibody syndrome, and Sjögren disease, among others, may

Case 1-8 A 44-year-old left-handed woman subacutely develops insomnia, decreased appetite, and weight loss. These symptoms evolve slowly over a 2-month period, until she abruptly loses the ability to read. This problem persists, and 1½ weeks later, the patient drives into a roadside ditch. She does not recall the event. After the accident, the husband notices that his wife has new problems, including walking into door frames, misnaming familiar coworkers, and eventually slurring her speech. Neurologic examination reveals a disoriented woman with poor attention. Short-term memory is impaired, and paraphasic substitutions are made during speech. Cranial nerves are intact. Motor examination reveals mild generalized weakness. She has diffuse hyperreflexia with bilateral upgoing toes. Her balance is impaired, and she requires mild assistance when walking. Brain MRI reveals bilateral, confluent subcortical T2 hyperintensities, sparing the basal ganglia. Cervical MRI is normal. CSF shows protein elevated at 74 mg/dl with 14 nucleated cells. Immune studies find no oligoclonal bands or elevated IgG index. Cerebral angiogram is normal. Brain biopsy is ultimately performed and reveals small vessel transmural inflammation in the brain. Comment. This patient was ultimately diagnosed with isolated CNS angiitis. Despite the multifocal neurologic symptoms spread over time and the discovery of white matter abnormalities on brain MRI, a number of red flags can be identified in her history that make MS less likely. Language impairment, loss of consciousness (possibly due to seizure), diminished attention, and confusion would all be unusual symptoms of MS, especially early in the disease course. The early confluent white matter disease and lack of oligoclonal bands or immunoglobulin elevation also suggest an alternative diagnosis. produce oligoclonal bands (Bourahoui et al, 2004). For this reason, excluding alternative inflammatory conditions is best accomplished using the clinical, laboratory, and radiologic techniques specific to those conditions. Case 1-9 A 24-year-old woman awakens one morning with sensory loss in her left face, arm, and leg. Brain MRI reveals two T2 hyperintensities concerning for demyelinating disease. Three months later, the morning after a long hike in hot weather, she awakens with a recurrence of left-sided symptoms, including weakness that makes her unable to stand on her own. Treatment with IV steroids does not improve her deficits, and the weakness only partially improves with time. Interferon beta-1a is then started. Three months later, a third episode occurs, and the patient awakens with right-sided numbness and footdrop. IV steroids again fail to help. The patient has a long history of migrainous headaches without aura. Several family members have similar headaches, including her mother, daughter, and a maternal uncle. Brain MRI is obtained after each attack, and new lesions appear on each scan. A large left anterior temporal lobe lesion is gadolinium enhancing, and corpus callosum lesions are also present. CSF contains 5 oligoclonal bands not present in serum but is otherwise normal. Due to her lack of response to IV steroids, the temporal lobe lesions on MRI, and the personal and family history of migraines, genetic testing for CADASIL is eventually performed and a CADASIL-consistent mutation is identified. Comment. In this case, the patient s clinical symptoms, MRI, and CSF were all consistent with relapsing MS. With time, clues to a possible misdiagnosis emerged, including steroid unresponsiveness, anterior temporal lobe lesions, and a prominent headache history in the patient and her family. Although an affected parent has not been identified, it should be remembered that CADASIL, like MS, can span a wide spectrum of clinical attacks and disability. Not every patient considered for CADASIL testing need have a prominent family history of strokes, migraine with aura, and dementia. The finding of oligoclonal bands in this case, although absent in 98% of subjects in one series, has been reported in at least one other case of CADASIL. 29

"DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS 30 KEY POINT: A It is better to delay making a diagnosis than to make a wrong diagnosis of MS. TABLE 1-10 Differential Diagnosis of Oligoclonal Bands and Increased Immunoglobulin G in CSF " Infectious Diseases Subacute sclerosing panencephalitis Viral encephalitis a HIV " Autoimmune Inflammatory Diseases Systemic lupus erythematosus Neuromyelitis optica a Acute disseminated encephalomyelitis a " Degenerative Diseases Adrenoleukodystrophy Cerebral autosomal dominant arteriopathy with subcortical infracts and leukoencephalopathy (uncommon) a Typically transient. Infectious disease, including various forms of viral encephalitis, neurosyphilis, Lyme disease, HIV, HTLV-1, and subacute sclerosing panencephalitis (in patients from countries where measles remains endemic), may also result in intrathecal immunoglobulin synthesis. ADEM may follow a seemingly innocuous infection and be associated with both imaging and MRI abnormalities resembling MS. CONCLUSIONS MS is a multifocal CNS disease that affects multiple brain or spinal cord regions over time. The space and time dissemination may be observed clinically or with the aid of ancillary diagnostic tools such as MRI, CSF, or VEPs. Since no single test is absolutely specific for MS, part of the diagnostic process involves ruling out potential mimics of MS and remaining alert to potential red flags (Table 1-11). MS mimics include infectious or autoimmune conditions, structural abnormalities, nutritional deficiencies, and genetic conditions. With a detailed history and examination, and careful consideration of the possible alternative explanations for MS-like symptoms, the chance of misdiagnosis is minimal. Given the many implications of a diagnosis of a chronic neurologic disease such as MS, it is better to delay making a diagnosis than to make the wrong diagnosis. TABLE 1-11 Red Flags " Hearing loss, especially bilateral " Onset age at the extremes <10 or >50 years old " CSF protein >100mg/dL " CSF white blood cell count >50 mm 3 " Presence of CSF polymorphonuclear cells " Negative brain MRI " Negative CSF (10% is negative) " Progressive-onset course (10% to 15% of cases) " Unilateral lesions on MRI brain " Coexisting systemic or autoimmune disorders " Prominent peripheral nervous system signs " Significant psychiatric history " Marked deficits without objective signs or MRI abnormalities

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