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Company Profile Company Profile GMbiolab Co., Ltd. was founded in 2001 in Taiwan. We provide a variety of molecular biology kits and reagents, including biological and biochemical materials, modified enzymes and nucleic acid products, which all are manufactured ourselves and distributed using our own brand - GeneMark. Strong technology capacities, advanced lab facilities and strict QC processes are our core capabilities. Nowadays, GeneMark is pretty popular in the domestic market for excellent quality, reasonable price and perfect after-sales services. Our customers are mainly life science researchers who perform a broad range of biological experiments in the laboratories generally associated with universities, medical research centers, government institutes, and other research institutes as well as biotechnology, agricultural, and pharmaceutical companies. Our vision is to be the most reputable and service-oriented leader in providing innovative solutions and technical support for customers in the life science field. The greatest goal is to achieve and maintain complete customer satisfaction and enhance our competitive force.

Contents 1. Molecular Biology Kits...... 1 2. PCR Related Products......44 3. Modified Enzymes......74 4. Restriction Enzymes. 83 5. Molecular Size Markers & Vector DNA...105 6. Biochemicals & Solutions....111 7. Plastic LabWares..... 135 8. Plant Virus Detection Kits.... 141 9. Custom Services 152

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits A. Plasmid DNA Purification Kits.. 2 B. DNA Clean up/ Gel Extraction Kits.....11 C. Genomic DNA Purification Kits...16 D. RNA Purification Kits.25 E. 96 well DNA & RNA Purification Kits.34 F. Protein Quantitation Assay Kits..42 1 GeneMark

Molecular Biology Kits Molecular Biology Kits Plasmid DNA Purification Kits Spin column format and easy to use High yield and high purity Speedy operation procedures Product Info Product Cat. No. Sample Volume (ml) DNA Recovery Application Time (min) GM Mini Plasmid Kit DP01m 1~5 ~25 µg General 10~15 Miniprep DP01 1~10 ~60 µg General 10~15 Miniprep-Plus DP01-Plus 1~10 ~60 µg Miniprep-II DP012 1~10 ~60 µg Remove Endotoxin, Transfection Remove Endonuclease 10~15 10~15 Midiprep DP01MD 20~100 ~300 µg Transfection 1 hr Midiprep-Plus DP01MD-P 20~200 ~500 µg Transfection 1 hr Maxiprep DP01MX 100~400 ~800 µg Transfection 2 hr Maxiprep-Plus DP01MX-P 200~800 ~1.5 mg Transfection 2 hr GMprep Yeast Plasmid DNA Kit GDP112-02 1~5 Varies depending on sample type and size General 2 hr GeneMark 2

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Easy to use: Spin column format. High purity: The ratio of A 260 to A 280 can reach 1.8~2.0. High recovery: Each reaction can obtain 2~25 μg ultra pure plasmid DNA (up to 20 kb) from1~5 ml culture. Fast: The procedures can be done within 10~15 minutes. GM Mini Plasmid Kit GM Mini Plasmid Kit is designed for purification of up to 25 μg plasmid DNA from 1~5 ml liquid culture. This purification system is based on spin column format, the simple procedure enables purification of high quality plasmid DNA, and is reproducible. The DNA quality is suitable for use in many downstream applications such as PCR, restriction digestion, cloning, automated DNA sequencing, Southern blot and in vitro transcription assay. Application The purified plasmid DNA can be used directly in subsequent applications such as PCR screens and bacterial transformations. A) 2.6 kb plasmid DNA M 1 2 3 4 5 B) 7.6 kb plasmid DNA M 1 2 3 4 5 C) 10 kb plasmid DNA M 1 2 3 4 5 Pellet 1~5 ml overnight culture Figure 1. Gel electrophoresis analysis of different sized plasmid DNA purified using GM Mini Plasmid Kit. 1 μl Plasmid DNA is loaded onto 1% agarose gel. Lane 1~5: Plasmid DNA extracted using GM Mini Plasmid Kit showed high extraction stability. M: GenKB DNA Ladder. Solution I, II, III treatment Bind Figure 2. Sequencing data of plasmid DNA purified using GM Mini Plasmid Kit. These sharp sequencing signals indicate that GM Mini Plasmid Kit can purify high purity plasmid DNA suitable for downstream experiments. Wash Elute Ordering Info DESCRIPTION Cat.No. REACTION GM Mini Plasmid Kit DP01m 50 GM Mini Plasmid Kit DP01m-300 300 High-purity plasmid DNA 3 GeneMark

Molecular Biology Kits Molecular Biology Kits Plasmid Miniprep Purification Kit Features High yield: Up to 60 μg plasmid DNA from 1~10 ml bacterial cultures. Fast: The whole process can be done within 10~15 minutes. Safe: Without phenolchloroform extraction or alcohol/isopropanol precipitation. Purity: The ratio of A 260 to A 280 can reach 1.8~2.0. Plasmid Miniprep Purification Kit contains a unique silica column and a highefficiency lysis buffer system that can yield up to 60 μg high purity plasmid DNA from 1 to 10 ml bacterial cultures. High-purity plasmid DNA can be obtained using this kit, without needing to precipitate, concentrate, or desalt. Application The buffer system does not affect downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, Southern blot, automated fluorescent sequencing, restriction digestion, ligation and transformation. M Q GM DNA Yield (μg) 15 10 5 0 Q GeneMark Pellet 1~5 ml overnight culture Solution I, II, III treatment Figure 1. Gel electrophoresis analysis of plasmid DNA purified using plasmid purification kit from GeneMark and supplier Q. M: Gen100 DNA Ladder Q: Supplier Q GM: GeneMark A260/280 1.83 1.95 Figure 2. Yield and purity measurements of plasmid DNA purified using GeneMark Plasmid Miniprep Kit and plasmid purification kit from supplier Q. Cultures of puc18 were aliquoted into three 1.5 ml tubes for test with GeneMark Plasmid Purification Kit and related kit from supplier Q separately. Data on O.D. measurements are the average of three preperations. Average yield was calculated from absorbance at 260 nm. Average purity was calculated by absorbance at 260 nm/280 nm. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Bind Wash Figure 3. Sequencing of plasmid DNA purified using GeneMark Plasmid Purification Kit. The clear and sharp signals of data indicate that reagents supplied in this kit do not affect downstream applications. Figure 4. Gel electrophoresis analysis of puc18 plasmid DNA (2.6 kb) purified using 15 randomly chosen spin columns. M: GenKB DNA Ladder 1~15: puc18 plasmid DNA Ordering Info Elute High-purity plasmid DNA DESCRIPTION Cat.No. REACTION Plasmid Miniprep Purification Kit DP01 50 Plasmid Miniprep Purification Kit DP01-300 300 GeneMark 4

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Plasmid Miniprep Plus Purification Kit Features High yield: Up to 60 μg endotoxin-free plasmid DNA from 1~10 ml bacterial cultures. Fast: The whole process can be done within 10~15 minutes. Safe: Without phenol-chloroform extraction or alcohol/isopropanol precipitation. High purity: The ratio of A 260 to A 280 can reach 1.8~2.0 and the endotoxins are removed effectively from samples. Pellet 1~10 ml overnight culture Plasmid Miniprep Plus Purification Kit contains a unique spin column and a high-efficiency lysis buffer system that can yield up to 60 μg high purity plasmid DNA from 1 to 10 ml bacterial cultures. This Miniprep Plus Kit contains endotoxin-removal reagent to wash off bacterial endotoxins and thus purified plasmid DNA is suitable for transfection experiments. Application The buffers in DP01-Plus do not affect downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, Southern blot, automated fluorescent sequencing (including capillary sequencing and radioactive sequencing), restriction digestion, ligation, transformation and transfection. Figure 1. Gel electrophoresis analysis of plasmid DNA isolated using GeneMark Plasmid Miniprep Plus Purification Kit and related kit from supplier Q. M: Gen100 DNA Ladder Q: Supplier Q GM: GeneMark Solution I, II, III treatment Ordering Info DESCRIPTION Cat.No. REACTION Bind Plasmid Miniprep Plus Purification Kit DP01-Plus 50 Plasmid Miniprep Plus Purification Kit DP01-Plus-300 300 Endotoxin Removal Wash Solution Wash Elute High-purity plasmid DNA 5 GeneMark

Molecular Biology Kits Molecular Biology Kits Features High yield: Up to 60 μg endonuclease free plasmid DNA from 1~10 ml bacterial cultures. Fast: The whole process can be done within 10~15 minutes. Safe : Without phenol-chloroform extraction or alcohol/isopropanol precipitation. Plasmid Miniprep Purification Kit II Plasmid Miniprep Purification Kit II is designed especially for endonuclease-rich bacteria strains (End A(+)) such as BL21(DE3), HB101 and JM109. It contains endonuclease-removal reagent, which eliminates endonuclease to protect plasmid DNA from degradation. Using the unique silica column and a highefficiency lysis buffer system, this kit can yield up to 60 μg high purity plasmid DNA from 1 to 10 ml bacterial cultures, without needing to precipitate, concentrate, or desalt. Application The buffer system does not affect the downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, Southern blot, automated fluorescent sequencing (including capillary sequencing and radioactive sequencing), restriction digestion, ligation and transformation. High purity: The ratio of A 260 to A 280 can reach 1.8~2.0. Pellet 1~5 ml overnight culture Solution I, II, III treatment M 1 2 3 Figure 1. Gel electrophoresis analysis of mini-preparation of pet29 recombinant in BL21 (DE3) with three trademark kits. After elution, add Hind III reaction buffer to final 1X concentration, incubate at 37 for 2 hours. M: GenKB DNA Ladder Lane 1: Purified using GeneMark Plasmid Miniprep Purification Kit II Lane 2: Using related product from supplier A Lane 3: Using related product from supplier B Bind Ordering Info DESCRIPTION Cat.No. REACTION Wash Solution A Plasmid Miniprep Purification Kit II DP012 50 Plasmid Miniprep Purification Kit II DP012-300 300 Wash Solution B Elute High-purity plasmid DNA GeneMark 6

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Yield: Up to 300 μg plasmid DNA from 20~100 ml bacterial cultures. Fast: Centrifugation format is used in this kit. The whole process can be done within an hour. Safe: Without phenol-chloroform extraction. Purity: The ratio of A 260 to A 280 can reach 1.8~2.0 and both endotoxins and endonuclease are removed effectively from samples. Plasmid Midiprep Purification Kit Plasmid Midiprep Purification Kit contains a unique Midi-spin column and a highefficiency lysis buffer system that can yield up to 300 μg of high purity plasmid DNA from 20 to 100 ml bacterial cultures. The buffer system removes both endotoxin and endonuclease, therefore, high-purity plasmid DNA purified using this kit is suitable for performing transformation or transfection experiments. Application The buffers provided in Plasmid Midiprep Purification Kit do not affect downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, Southern blot, automated fluorescent sequencing (including capillary sequencing and radioactive sequencing), plasmid-mediated gene silencing, restriction digestion, ligation, transformation, and transfection. (a) M Q GM (a) M Q GM Pellet 20~100 ml overnight culture Solution I, II, III treatment (b) 250 200 150 100 50 0 DNA Yield (μg) Q GeneMark (b) 120 100 80 60 40 20 0 Q DNA Yield (μg) GeneMark Isopropanol precipitation Bind Endotoxin Removal Wash Solution A260/280 1.87 1.91 Figure 1. 6.3 kb plasmid DNA was purified from 40 ml culture using GeneMark Plasmid Midiprep Purification kit and related kit from supplier Q. (a) Gel electrophoresis analysis of plasmid (b) DNA using 0.8% agarose gel. O.D. Measurement of purified plasmid DNA in wave length 260 nm and 280 nm. M: GenKB DNA Ladder Q: Supplier Q GM: GeneMark A260/280 1.87 1.89 Figure 2. 10 kb plasmid DNA was purified from 40 ml culture using GeneMark Plasmid Midiprep Purification kit and related kit from supplier Q. (a) Gel electrophoresis analysis of plasmid DNA using 0.8% agarose gel. (b) O.D. Measurement of purified plasmid DNA in wave length 260 nm and 280 nm. M: GenKB DNA Ladder Q: Supplier Q GM: GeneMark Wash Ordering Info Elute DESCRIPTION Cat.No. REACTION Plasmid Midiprep Purification Kit DP01MD 20 Plasmid Midiprep Purification Kit DP01MD-100 100 7 GeneMark

Molecular Biology Kits Molecular Biology Kits Features High yield: Up to 300 μg endotoxin-free Plasmid Midiprep Plus Purification Kit The Plasmid Midiprep Plus Purification Kit provides simple rapid spin-column protocol and materials to purify plasmid DNA from 20~200 ml of liquid bacterial culture. The yield of plasmid DNA, from 100 μg~500 μg, is quantitative and reproducible. Differ from Plasmid Midiprep Purification Kit, this kit provides 50 ml spin-column for sample treatments. Application The buffers supplied in this kit do not affect downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, restriction digestion, ligation, Southern blot, automated fluorescent sequencing (including capillary sequencing and radioactive sequencing), plasmidmediated gene silencing, transformation, and transfection. M DP01MD DP01MD-P DP01MD DP01MD-P Yield (μg) 283.82 486.38 Conc. (ng/μl) 296.33 415 A 260 /A 280 1.89 1.83 A 260 /A 230 2.39 2.29 Pellet 20~200 ml overnight culture Solution I, II, III treatment Figure 1. GeneMark Plasmid Midiprep Purification Kit (DP01MD) and GeneMark Plasmid Midiprep Purification Kit Plus (DP01MD-P) were used to purify Ava24-89 plasmid DNA from 100 ml bacteria culture. M: Gen-kb DNA Ladder DP01MD:Plasmid Midiprep Purification Kit DP01MD-P: Plasmid Midiprep Purification Plus Kit Filter Net Endotoxin Removal Wash Solution Ordering Info DESCRIPTION Cat.No. REACTION Plasmid Midiprep Plus Purification Kit DP01MD-P10 10 Plasmid Midiprep Plus Purification Kit DP01MD-P50 50 Wash Elute Ultrapure plasmid DNA GeneMark 8

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features High yield: Up to 800 μg endotoxin-free plasmid DNA from 100~400 ml bacterial cultures. Fast: All steps including washing and centrifugation are carried out in a 50 ml tube, and can be done within 2 hours. Safe: Without phenol-chloroform extraction or CsCl / ethidium bromide gradient centrifugation. Plasmid Maxiprep Purification Kit Plasmid Maxiprep Purification Kit provides a unique Maxi - column and a high-efficiency lysis buffer system that can yield up to 800 μg high purity plasmid DNA from 100 to 400 ml bacterial cultures. The system contains endotoxin- removal reagent to eliminate endotoxins. The high-purity plasmid DNA eluted using Plasmid Maxiprep Purification Kit is suitable for performing transfection experiments. Application The buffers supplied in this kit do not affect downstream applications. The purified plasmid DNA can be used for subsequent applications such as PCR, restriction digestion, ligation, Southern blot, automated fluorescent sequencing (including capillary sequencing and radioactive sequencing), plasmid-mediated gene silencing, transformation, and transfection. Purity: The ratio of A 260 to A 280 can reach 1.8~2.0 and endotoxins can be removed effectively from samples. (a) M 1 2 3 4 5 (b) No. 260/280 260/230 Total yield (μg) 1 1.89 2.22 1016 2 1.92 2.28 904.4 3 1.91 2.32 850 4 1.86 2.26 930 5 1.87 2.22 960 Average yield (μg) 932.08 Pellet 100~400 ml overnight culture Solution I, II, III treatment Figure 1. 4.6 kb plasmid DNA purified from 120 ml of E.coil using Plasmid Maxiprep Purification Kit. (a) The plasmid DNA was eluted using 1.5 ml of Elution buffer and analyzed in 0.8% agarose gel. (b) O.D. measurement of purified plasmid DNA in wave length 230 nm, 260 nm and 280 nm. Figure 2. Western blot analysis of transfection with pakt plasmids, purified using two trademark endotoxin-free plasmid maxiprep kits. Lane 1: BHK-21 cell lysate only. Lane 2: Transfect vector only. Lane 3: Transfect pakt plasmid using kit from supplier Q. Lane 4: Transfect pakt plasmid using kit from GeneMark. Filter Net Endotoxin Removal Wash Solution Wash Ordering Info Yield up to 800 μg from 100~400 ml culture DESCRIPTION Cat.No. REACTION Plasmid Maxiprep Purification Kit DP01MX-10 10 Plasmid Maxiprep Purification Kit DP01MX-20 20 Elute Ultrapure plasmid DNA Yield up to 1.5 mg from 200~800 ml culture Plasmid Maxiprep Plus Purification Kit DP01MXP-10 10 Plasmid Maxiprep Plus Purification Kit DP01MXP-20 20 9 GeneMark

Molecular Biology Kits Molecular Biology Kits GMprep Yeast Plasmid DNA Kit GMprep Yeast Plasmid DNA Kit is designed for rapid isolation of plasmid DNA from yeast cultures. This kit uses a simple, highly efficient method for yeast plasmid mini-preparation. No glass beads and phenol is needed. The same kit can be used to purify plasmid DNA from recombinant bacteria for use in any subsequent applications. Features Fast and Convenient: Rapid isolation of plasmid DNA from yeast cultures. Yield: 6~7 µg of DNA from 1 ml sample, varies depending on sample type and size. Application The purified plasmid DNA can be used directly in subsequent applications such as PCR screens and bacterial transformations. Ordering Info DESCRIPTION Cat.No. REACTION GMprep Yeast Plasmid Kit GDP112-02 50 GeneMark 10

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits DNA Clean up/ Gel Extraction Kits Stable, easy to use Qualified Better performance Product Info Product Cat. No. Elution volume DNA binding Recovery rate Gel elution DP03 30~100 µl 20 µg 60~90% PCR Clean-up DP04 30~100 µl 20 µg 80~95% DNA Clean / Extraction DP034 30~100 µl 20 µg Gel: 60~90% Liquid: 80~95% Micro-Elute DNA Clean / Gel extraction DP034ME As little as 10 µl > 20 µg Gel: >80% Liquid: 80~95% 11 GeneMark

Molecular Biology Kits Molecular Biology Kits Features High capacity and recovery rate: The DNA binding capacity is up to 20 μg per spin column. 60~90% recovery is achieved, depending on DNA fragment size. Fast: After dissolving the gel slice, the easy bindingwash-elute procedure can be completed within 15 minutes. Gel Elution Kit 10 kb 6 kb The Gel Elution Kit is designed to extract DNA fragments of 70 bp~50 kb from standard or low-melting gels in either Tris acetate (TAE) or Tris borate (TBE) buffer system. The kit uses silica spin-column format and can elute DNA fragments from gel slices up to 250 mg in size. After the easy bind-wash-elute procedure, the recovery rate is 60%~90% depending on the sizes of DNA fragments. Application Purified DNA can be used directly for subsequent applications such as cloning, automated fluorescent DNA sequencing, labeling, restriction enzyme digestion or in vitro transcription/translation. 1 2 3 4 5 6 7 8 9 10 3 kb 250 mg gel slice Binding Solution 60, 5~15 min Figure 1. Gel extraction of 3 kb, 6 kb and 10 kb DNA fragments using three trademark gel elution kits. Lane 1: Unpurified DNA fragments Lane 2, 5, 8: Gel extraction using GeneMark Gel Elution kit Lane 3, 6, 9: Gel extraction using related kit from supplier A Lane 4, 7, 10: Gel extraction using related kit from supplier B Bind Ordering Info DESCRIPTION Cat.No. REACTION Gel Elution Kit DP03 50 Wash Gel Elution Kit DP03-300 300 Elute Ultrapure DNA GeneMark 12

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits PCR Clean-Up Kit Features High capacity and recovery rate: The DNA binding capacity is up to 20 μg per column. 80~95% recovery rate can be achieved. The PCR Clean-Up Kit is designed to purify DNA fragments from PCR products or other enzymatic reaction (eg. Enzyme digestion, DNA ligation, probe labeling) based on our specific buffer system and high-capacity column. After the easy bind-wash-elute procedure, excess primers and salts are removed from PCR products or enzymatic reaction, and DNA fragments larger than 70 bp (up to 50 kb) can be purified using this kit. Application DNA purified can be used directly for subsequent applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation. Fast: The whole process can be done within 15 minutes. 300 bp 1 kb 1 2 3 4 1 2 3 4 Figure 1. Gel electrophoresis analysis of PCR reaction before (1) and after (2),(3),(4) purification using GeneMark PCR Clean-Up Kit and related kit from commercial suppliers. Lane 1: Unpurified PCR product Lane 2: Purified using GeneMark PCR clean-up kit Lane 3: Purified using related kit from supplier A Lane 4: Purified using related kit form supplier B PCR product or other DNA solution Ordering Info DESCRIPTION Cat.No. REACTION Binding Solution PCR Clean-Up Kit DP04 50 PCR Clean-Up Kit DP04-300 300 Bind Wash Elute Ultrapure DNA 13 GeneMark

Molecular Biology Kits Molecular Biology Kits Features High capacity and recovery rate: The DNA binding capacity is up to 20 μg per column. The recovery rate is 80%~95% for PCR clean-up and 60%~90% for DNA gel elution, depending on size of DNA fragments. Fast: The whole process can be done within 15 minutes. DNA Clean/Extraction Kit (PCR Clean-up/ Gel Extraction/ DNA Clean-Up) DNA Clean/Extraction Kit is designed to extract DNA fragments of 70 bp to 50 kb from standard or low-melting agarose gels in either Tris acetate (TAE) or Tris borate (TBE) buffer system, and can also purify DNA fragments directly from an amplification or enzymatic reaction based on our specific buffer system. This kit can be used for DNA gel elution, PCR clean-up, and DNA desalting. Applications DNA purified can be used directly for subsequent applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation. Gel Elution 500 bp and 6 kb of PCR products were purified by gel elution method. DNA Clean up 500 bp and 6 kb of PCR products were purified by clean-up method. M O GM Q M O GM Q M O GM Q M O GM Q 250 mg gel slice PCR product or other DNA solution 500 bp 6 kb O: Original PCR product Q: Supplier Q GM: GeneMark DNA clean/ Extraction Kit 500 bp 6 kb O: Original PCR product Q: Supplier Q GM: GeneMark DNA clean/ Extraction Kit Binding Solution 60, 5~15min Binding Solution Bind Primer Removal Remove oligo-primers of 50 bases and 33 bases using GeneMark DNA Clean/Extraction and elution product from supplier Q. M O GM Q M O GM Q Wash 50 mer, 0.5 μg 33 mer, 1 μg O: Original PCR product Q: Supplier Q GM: GeneMark DNA clean/ Extraction Kit Ordering Info Elute Ultrapure DNA DESCRIPTION Cat.No. REACTION DNA Clean/Extraction Kit DP034 50 DNA Clean/Extraction Kit DP034-300 300 GeneMark 14

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features High capacity and recovery rate: 1 μl silica matrix can bind at least 2 μg DNA. The recovery rate can reach 95% for PCR clean-up and 80% for DNA gel elution, depending on DNA fragment size. DNA concentration: DNA can be eluted in small volumes as little as 10 μl. Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/ Gel Extraction/ DNA Clean-Up/ DNA Concentration) The Micro-Elute DNA Clean/Extraction Kit provides silica matrix with ultrahigh binding capacity and unique buffer system to recover DNA in small volumes (as little as 10 μl). This design provides highly concentrated DNA in high yields when proceeding DNA gel elute, PCR clean-up, or DNA desalting. Applications DNA purified can be used directly for subsequent applications such as automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation. A M 1 2 3 M 1 2 3 B Fast: The whole process can be done within 15 minutes. 500 mg gel slice Binding Solution Silica matrix 60, 5-15min PCR product or other DNA solution Binding Solution Silica matrix Wash (Spin filter) Figure 1. Concentrating DNA and gel purification using GeneMark Micro-Elute DNA Clean/Extraction kit. A: DNA Clean-up Lane 1: 1 l of unpurified PCR 300 bp product Lane 2: 10 μl of PCR product was diluted to 1 ml with TE buffer, then processed and eluted to 10 μl using Micro-Elute DNA Clean/Extraction kit. 1 l of the eluate was loaded onto agarose gel. Lane 3: Concentrate 500 l of PCR product to 10 l volume. Load 1 l of eluate onto agarose gel. B: Gel purification Lane 1: 5 l of unpurified PCR product Lane 2: 50 l eluate of 450 bp DNA fragment was recovered from gel slice by GeneMark DNA Clean/ Extraction kit. Load 5 l of eluate on agarose gel. Lane 3: 10 l eluate of 450 bp DNA fragment was recovered from gel slice by Micro- Elute DNA Clean/Extraction kit. Load 1 l of eluate on agarose gel. Elute (10~20 μl elution solution) Ordering Info Ultrapure DNA DESCRIPTION Cat.No. REACTION Micro-Elute DNA Clean/Extraction Kit DP034ME 50 Micro-Elute DNA Clean/Extraction Kit DP034ME-300 300 15 GeneMark

Molecular Biology Kits Molecular Biology Kits Genomic DNA Purification Kits Spin column format and easy to use High yield and high purity Speedy operation procedure Product Info Product Cat. No. Sample type and volume DNA recovery Format Time Tissue/Cell EASY Tissue/Cell DP021 Bacteria, animal cells & tissues, 20~40 µg Spin column 30 min insect,spleen, mouse tail, yeast DP021E ~80 µg Salting-out 20 min Plant DP022 Arabidopsis thaliana, rice, tomato, tobacco, 3~50 µg Spin column 2 hrs orchid, algae, fungi, medical herbs or viral Midi Plant DP022MD DNA from infected plant tissue/cells 30~500 µg Salting-out 2 hrs Blood DP023 Whole Blood up to 1.5 ml 3~50 µg Spin column 30 min EASY Blood DP023E Whole Blood up to 3 ml ~60 µg Salting-out 20 min GMrelax Blood GDP319 Whole Blood up to 20 ml ~400 µg Salting-out 30 min New Products Product Cat. No. Sample type and volume Format Bacteria DP025 Bacteria cells up to 2 x 10 9 Spin column Yeast DP026 Yeast cells up to 5 x 10 7 Spin column GMamp Swab DNA GDP322-02 Swab samples Spin column GeneMark 16

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features: Sample type: Bacteria, animal cells & tissues, insects, nematodes, mouse tail, yeast, etc. Sample size: 25 mg tissue or 5 x 10 6 cells. Yield: The purified genomic DNA is up to 50 kb in size and yields from 20 to 40 μg depending on sample types. Fast: The bacterial genomic DNA can be obtained within an hour. May take longer for some samples due to different lysis time. Safe: Without phenol-chloroform extraction. Purity: The ratio of A 260 to A 280 is between 1.7 and 1.9. Tissue & Cell Genomic DNA Purification Kit Tissue & Cell Genomic DNA Purification Kit simplifies purification of DNA from animal tissues, cells, bacteria, nematodes, and yeast with three easy steps: cell lysis, DNA binding, and DNA elution. Applications The purified genomic DNA can be used for subsequent applications such as PCR, Real-Time PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion, cloning etc. /HindIII 1 2 3 4 5 Figure 1. Gel electrophoresis analysis of purified genomic DNA from various samples using GeneMark Tissue & Cell Genomic DNA Purification Kit. 2% of purified DNA (left) and EcoRI digested DNA (right) were loaded on 1% agarose gel. Lane 1: DH5 ( E.coli ) Lane 2: Bladder cells (cultured cells) Lane 3: Mouse tail Lane 4: Zebrafish tail fin Land 5: Cricket (whole insect) Animal tissue, cell culture, bacteria Lyse Ordering Info DESCRIPTION Cat.No. REACTION Tissue & Cell Genomic DNA Purification Kit DP021 50 Tissue & Cell Genomic DNA Purification Kit DP021-150 150 Bind Wash Elute Ultrapure genomic DNA 17 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Sample type: Bacteria, animal cells & tissues, insects, nematodes, mouse tail, yeast, etc. Sample size: 20 mg tissue or 1 x 10 7 cells. Yield: ~80 μg genomic DNA can be obtained depending on sample types. Fast: Easy steps without spin column cell lysis, proteinase K digestion, protein precipitation, and DNA precipitation. Safe: Without phenolchloroform extraction. Animal tissue, cultured cells, bacteria Easy Tissue & Cell Genomic DNA Purification Kit Easy Tissue & Cell Genomic DNA Purification Kit provides a simple solution based procedure without spin column and phenol-chloroform extractions. No hazardous chemicals are contained in the lysis buffer. The high-yield genomic DNA is obtained by centrifugation and precipitation method throughout the whole procedure, which involves cell lysis/proteinase K digestion, protein precipitation and DNA precipitation. Application The purified genomic DNA can be used for subsequent PCR, Southern blot, DNA hybridization, restriction digestion, cloning, etc. M 1 2 3 4 Figure 1. Purification of zebrafish gdna using GeneMark Easy Tissue & Cell Genomic DNA Purification Kit. 1% of purified DNA eluate was loaded on 1% agarose gel. Lane 1, 2, 3: Purification of zebrafish gdna from 15 mg, 8 mg, 5 mg of whole zebrafish Lane 4: Zebrafish tail-fin gdna M: GenKB DNA Ladder. Lyse Ordering Info Protein Precipitation DESCRIPTION Cat.No. REACTION Easy Tissue & Cell Genomic DNA Purification Kit DP021E 50 Isopropanol extraction Easy Tissue & Cell Genomic DNA Purification Kit DP021E-150 150 Wash pellet with 70% Ethanol Pure genomic DNA GeneMark 18

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Sample type: Plant samples such as rice, tomato, arabidopsis, algae, tobacco, orchids, fungus or virus infected plants. Sample size and Yield: Up to 50 μg genomic DNA from 50~100 mg plant tissues. Fast: The whole process can be done within 2 hours. Safe: Without phenol-chloroform extraction. Plant Genomic DNA Purification Kit Plant Genomic DNA Purification Kit is extremely suitable for purification of genomic DNA from various kinds of plants and fungi, especially plants rich in polysaccharide and secondary metabolites. The unique Precipitation Solution in the kit is designed to eliminate these contaminants easily to obtain high-quality gdna. Application The purified genomic DNA can be used for subsequent applications such as PCR, Real-Time PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion, cloning, etc. λ/hindiii Tobacco Cabbage Pine Orchid Aubergine Wheat Purity: Ultrapure genomic DNA without polysaccharide and secondary metabolites is obtained by silica-based spin column. The ratio of A 260 to A 280 is between 1.7 and 1.9. Various plant tissue (< 100 mg) Extraction Solution A Extraction Solution B RNase A Precipitation Solution Spin filter Figure 1. Gel electrophoresis analysis of DNA purified from the indicated leaves or needles using Plant Genomic DNA Purification kit. 2% of purified DNA eluate (left) and EcoRI digested DNA (right) were loaded on 1% agarose gel. Ordering Info DESCRIPTION Cat.No. REACTION Mix Wash (spin column) Plant Genomic DNA Purification Kit DP022 50 Plant Genomic DNA Purification Kit DP022-150 150 Wash (spin column) Elute Ultra pure Plant Genomic DNA 19 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Sample type: Plant samples such as rice, arabidopsis, algae, tomato, tobacco, orchids, fungus and virus infected plants. Sample size: Up to 1 g plant tissues. Yield: 30~500 μg genomic DNA. Fast: The whole process can be done within 2 hours. Safe: Without phenolchloroform extraction. Various plant tissues (250 mg ~ 1 g) Extraction Solution A Extraction Solution B RNase A Precipitation Solution Filter Net Midi Plant Genomic DNA Purification Kit Midi Plant Genomic DNA Purification Kit provides a simple solution-based procedure that is modified from salt precipitation solutions for polysaccharide-rich plants and for plant tissues rich in secondary metabolites. The procedure includes cell lysis, protein precipitation, polysaccharide and secondary metabolites removal, and gdna precipitation with isopropanol. The kit can process up to 1 g of plant tissue. Application The purified genomic DNA can be used for subsequent applications such as PCR, Southern blot, DNA hybridization, restriction digestion, cloning, etc. M 1 2 3 Figure 1. Gel electrophoresis analysis of plant genomic DNA purified from rice leaves using GeneMark Midi Plant Genomic DNA Purification Kit. 1% of purified DNA eluate was loaded on 1% agarose gel. Lane 1: 1 g of young rice leaf purified using GeneMark Midi Plant Genomic DNA Purification Kit Lane 2: 100 mg of young rice leaf using spin column kit from supplier A Lane 3: 100 mg of young rice leaf using spin column kit from supplier B Ordering Info DESCRIPTION Cat.No. REACTION Midi Plant Genomic DNA Purification Kit DP022MD 10 Midi Plant Genomic DNA Purification Kit DP022MD-50 50 Isopropanol extraction Wash pellet with 70% Ethanol Ultra Pure Plant genomic DNA GeneMark 20

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Sample type: Whole blood, plasma, serum and buffy coat. Sample size: 200 μl~1.5 ml of whole blood. Yield: 3~50 μg genomic DNA can be obtained from 500 μl whole blood. Fast: The whole process can be done within 30 minutes. Blood Genomic DNA Purification Kit Blood Genomic DNA Purification Kit provides an easy three-step procedure: cell lysis, DNA binding, and DNA elute, to isolate up to 50 μg of genomic DNA from 500 μl of whole blood, plasma, serum and buffy coat. The process is based on a spin column format to obtain high-purity genomic DNA. Application The purified genomic DNA can be used for subsequent applications such as PCR, Real-Time PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion, cloning, etc. /HindIII 1 2 3 Safe: Without phenol-chloroform extraction. Purity: The ratio of A 260 to A 280 is between 1.7 and 1.9. Figure 1. Gel electrophoresis analysis of genomic DNA purified from rabbit whole blood using GeneMark Blood Genomic DNA Purification Kit. 1% purified DNA was loaded on 1% agarose gel. Lane 1: 200 l whole blood purified with protocol A Lane 2: 500 l whole blood purified with protocol B Lane 3: 1.5 ml whole blood purified with protocol C < 200 μl whole blood, plasma, serum 200~500 μl whole blood, Buffy Coat (from <1.5 ml whole blood) Ordering Info Proteinase K 1X RBC lysis buffer Proteinase K DESCRIPTION Cat.No. REACTION Blood Genomic DNA Purification Kit DP023 50 Blood Genomic DNA Purification Kit DP023-150 150 Bind Wash Elute Ultrapure genomic DNA 21 GeneMark

Molecular Biology Kits Molecular Biology Kits Easy Blood Genomic DNA Purification Kit Features Sample type: Whole blood and buffy coat. Sample size: Up to 3 ml of whole blood. Yield: ~60 μg genomic DNA can be obtained from 500 μl whole blood. Fast: The whole process can be done within 20 minutes. Safe: Without phenolchloroform extraction. 500 μl whole blood, Buffy Coat (from 3 ml whole blood) Easy Blood Genomic DNA Purification Kit provides a simple solution-based procedure which is modified from salt precipitation solutions. The procedure includes RBC lysis, protein precipitation, DNA precipitation and DNA elute, which can yield ~60 μg genomic DNA from 500 μl whole blood. Application The purified genomic DNA can be used for subsequent applications such as PCR, Southern blot, DNA hybridization, restriction digestion, cloning, etc. M 1 2 3 Figure 1. Gel electrophoresis analysis of genomic DNA purified from rabbit whole blood using GeneMark Easy Blood Genomic DNA Purification Kit. 1% purified DNA were loaded on 1% agarose gel. Lane 1: 200 l of whole blood by Supplier A using spin column. Lane 2: 500 l of whole blood by Supplier A using spin column. Lane 3: 500 l of whole blood by GeneMark Easy Blood Genomic DNA Kit. 1X RBC lysis buffer Proteinase K Extraction Solution Protein Precipitation Solution Ordering Info DESCRIPTION Cat.No. REACTION Easy Blood Genomic DNA Purification Kit DP023E 50 Easy Blood Genomic DNA Purification Kit DP023E-150 150 Isopropanol extraction Wash pellet with 70% Ethanol Pure genomic DNA GeneMark 22

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits GMrelax Blood DNA System GMrelax Blood DNA system uses simple centrifugation method to purify genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. No organic extraction or other toxic reagents are necessary. A simple alcohol precipitation is used to recover high yields of pure DNA. Features Fast: High-quality purified DNA could be provided in about 1~2 hours. Application The purified genomic DNA can be used for subsequent applications such as PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion, cloning, etc. Safe: Without phenol-chloroform extraction. Figure 1. Genomic DNA purified using GMrelax Blood DNA System. Genomic DNA was purified from 400 μl old blood samples. 3 μl of 100 μl eluates (in TB buffer) were loaded per lane. Sample Sample Volume DNA Yield Mammalian Blood 300 µl 1 ml 5 ml 20 ml 3~10 µg 4~30 µg 100~200 µg 300~700 µg Avian Blood 5~20 µl 5~40 µg Coagulated Blood 200~500 µl 500~1000 µl 1~8 µg 8~15 µg Ordering Info DESCRIPTION Cat.No. PACKAGE GMrelax Blood DNA System (0.1~20 ml) GDP319-01 50 ml GMrelax Blood DNA System (0.1~20 ml) GDP319-02 200 ml 23 GeneMark

Molecular Biology Kits Molecular Biology Kits GMamp Swab DNA Kit Features Silica Membrane Technology: The kit ensures rapid purification of highquality DNA. GMamp Swab DNA Kit uses silica membrane technology and special buffer system for gdna extraction. The spin column made of new type silica-gel membrane in Swab DNA Kit can be easily bounded by DNA specifically. Genomic DNA from swab samples can be purified in 90 minutes. Purified DNA is of high quality and serves as an excellent template for agarose gel analysis, restriction enzyme digestion, PCR analysis and blotting procedures. Application The purified genomic DNA can be used for PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion, cloning, etc. Yield: 0.5~3.5 μg genomic DNA can be isolated from one swab sample. Sample Sample Quantity DNA Yield Buccal Swab 1 0.5~3.5 μg Figure 1. Genomic DNA purified from swab samples using GMamp Swab DNA Kit. Genomic DNA was purified from different swab samples. 3 μl of 50 μl eluates (in TB buffer) were loaded per lane. Ordering Info DESCRIPTION Cat.No. REACTION GMamp Swab DNA Kit GDP322-02 50 GMamp Swab DNA Kit GDP322-03 200 GeneMark 24

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits RNA Purification Kits Spin column format and easy to use High yield and high purity Product Info Speedy operation procedure Product Cat. No. Sample RNA Recovery Format Time Total RNA Purification Kit TR01 Animal cells and tissue, bacteria ~100 µg Spin Column 1 hr Plant Total RNA Purification Kit TR02 Herb and woody plants, fungi ~100 µg Spin Column 1 hr GMamp Virus RNA GDP315-R Whole blood, plasma, serum and other cellfree materials Varies depending on sample type Spin Column 1 hr GMamp Virus DNA/RNA GDP315 Whole blood, plasma, serum and other cellfree materials Varies depending on sample type Spin Column 1 hr GMprep DNA/RNA Isolation GDP422 Animal cells and tissue Varies depending on sample type Spin Column 40 min New Products Product Cat. No. Sample Format Blood RNA Miniprep TR03 Whole Blood samples Spin column Bacteria RNA Miniprep TR05 Bacteria Cells Spin column Yeast RNA Miniprep TR06 Yeast Cells Spin column Single Reagent Products Product Cat. No. Sample Trisolution plus TS-100-Plus Cells, tissues, bacteria, plant, yeast and virus GRNzol Reagent GDP405 Cells and tissues GRNzol-A+ Reagent GDP421 Cells and tissues 25 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Yield: 10~100 μg ultra pure total RNA depending on the sample type and sample size. Purity: High-quality total RNA is purified using spin column format. RNasefree DNase I is included in the kit to digest contaminating gdna in RNA preparation. Total RNA Purification Kit The Total RNA Purification Kit provides specific buffer system and highcapacity binding glass fiber matrix column to purify high purity RNA from bacteria, animal/plant tissues and cells. Application Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Non-denaturing gel electrophoresis analysis of total RNA purified from various samples using GeneMark Total RNA Purification Kit and related kit from supplier Q. The RNA purified using related kit from supplier Q contains large amount of genomic DNA contamination. Fast: RNA can be obtained within an hour. Safe: No phenol / chloroform extraction or alcohol/ isopropanol precipitation. Figure 2. RT-PCR results of RNA purified using GeneMark Total RNA Purification Kit and related kit from supplier Q. Total RNA was purified from zebrafish and zebrafish EF-1α gene was amplified. Genomic DNA contamination is observed in RNA extracted using related kit from supplier Q by Taq only control. Animal tissue, cell culture, bacteria Lyse (Add 100% EtoH) Brand GeneMark A260/A280 1.90 1.97 Total Yield (μg) (average) 378 RNA Wash Solution I Q 2.00 1.85 318.5 DNase I treatment Figure 3. Total RNA of E.coli DH5α purified using GM Total RNA Purification Kit and purification kit from supplier Q. Total RNA of E.coli DH5α was purified from 1 ml culture and eluted in 50 μl RNase-free H 2 O. 2.5 μl of RNA were loaded onto each lane of agarose gel. GeneMark: TR01 Q: supplier Q RNA Wash Solution I RNA Wash Solution II (2 times) Elute Ultrapure total RNA Ordering Info DESCRIPTION Cat.No. REACTION Total RNA Purification Kit TR01 50 Total RNA Purification Kit TR01-150 150 GeneMark 26

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Yield: 10~100 μg ultra pure total RNA depending on the sample type and sample size. Purity: High-quality total RNA is purified using glass fiber matrix column. Plant contaminants such as polyphenolics and polysaccharides can be removed by the kit; DNase I is also included to digest contaminating DNA during RNA preparation. Fast: RNA can be obtained within an hour. Safe: No phenol/chloroform extraction or alcohol/ isopropanol precipitation. Plant Total RNA Purification Kit Plant Total RNA Purification Kit is designed for herbs, woody plants, succulent plants, and fungi. This kit provides two alternative lysis system, Lysis solution A (guanidine thiocyanate) and Lysis solution B (SDS/anti-oxifants). Choose the most suitable lysis system combining with high-capacity binding glass fiber matrix column to purify the total RNA from a wide variety of plant and fungal samples. Application Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Non-denaturing gel electrophoresis analysis of the total RNA purified from various plant leaf samples using GeneMark Plant Total RNA Purification Kit with Lysis Solution B system, and related kit from supplier Q. The rice RNA purified using related kit from supplier Q contains large amount of genomic DNA contamination. Plant tissue Lyse Spin filter Add 100% EtoH or binding buffer RNA Wash Solution I (spin column) RT-PCR 0.9 kb gdna PCR 1.2 + 1.3 kb Figure 2. Gel electrophoresis analysis of RT-PCR of rice actin gene. Total RNA was purified using GeneMark Plant Total RNA Purification Kit and related kit from supplier Q. Genomic DNA contamination was observed from RNA purified using kit from supplier Q in both RT and Taq only control. DNase I treatment Ordering Info RNA Wash Solution I RNA Wash Solution II (2 times) DESCRIPTION Cat.No. REACTION Plant Total RNA Purification Kit TR02 50 Plant Total RNA Purification Kit TR02-150 150 Elute Ultrapure plant total RNA 27 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Fast: High-quality viral total RNA can be obtained within an hour. Sample Volume: 140 μl~560 μl liquid sample is accepted. GMamp Virus RNA Kit GMamp Virus RNA Kit provides a fast, simple, and cost-effective viral RNA miniprep method for viral RNA purification from whole blood, plasma, serum and other cell-free materials. GMamp Virus RNA Kit uses silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Carrier RNA in the kit improves the binding of viral RNA to the GMamp membrane especially in the case of low-titer samples, and limits possible degradation of the viral RNA due to any residual RNase activity. Viral RNA purified using GMamp Virus RNA Kit is immediately ready for use. Phenol extraction and ethanol precipitation are not required. High-quality viral RNA is eluted in a small volume of RNase-free ddh 2 O. Application Viral (HCV) genotyping research, viral epidemiologic study, viral infectious diseases analysis. Safe: No phenol/chloroform extraction and no CsCl gradients, no LiCl or ethanol precipitation. A B Sample Duplicate CT value Average Sample Duplicate CT value Average 1 Duplicate 1 19.71 Duplicate 2 19.638 19.70 1 Duplicate 1 26.44 Duplicate 2 126.35 26.40 2 Duplicate 1 19.8 Duplicate 2 19.75 19.78 2 Duplicate 1 26.37 Duplicate 2 26.35 26.36 3 Duplicate 1 19.49 Duplicate 2 19.56 19.53 3 Duplicate 1 26.33 Duplicate 2 26.36 26.35 NTC None - Average 19.67 NTC None - Average 26.37 Figure 1. Virus Genomic DNA purified using GMamp Virus DNA/RNA Kit. Genomic DNA was purified form 200 μl of Avian influenza virus standard antigen that was diluted 100 times (A), or 10,000 times (B). 4 μl of 50 μl eluates were used as template in real-time PCR detection. Real-time PCR were in triplicate for each sample. Ordering Info DESCRIPTION Cat.No. REACTION GMamp Virus RNA Kit GDP315-R 50 GeneMark 28

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits GMamp Virus DNA/RNA Kit Features Fast: High-quality viral genomic DNA or total RNA can be obtained within an hour. Sample Volume: 200 μl liquid sample is accepted. GMamp virus DNA / RNA kit provides a fast, simple, and cost-effective virus DNA/RNA miniprep method for virus DNA or RNA purification from plasma, serum and other cell-free materials. GMamp virus DNA / RNA kit is based on silica membrane technology and unique buffer system. Carrier RNA in the kit improves the binding of virus RNA to the membrane especially in the case of low-titer samples, and limits possible degradation of the virus RNA due to any residual RNase activity. High-quality virus DNA / RNA is eluted in a small volume of Rnase-free ddh 2 O. Application The purified DNA/RNA is ready for use in subsequent applications such as PCR, RT-PCR, real-time PCR, nest PCR, etc. Viral (HCV) genotyping research, viral epimiologic study, viral infectious diseases analysis. Safe: No phenol/chloroform extraction and no CsCl gradients, no LiCl or ethanol precipitation. Figure 1. Viral DNA purified using GMamp Virus DNA/RNA Kit. Viral DNA was purified from serum samples containing Hepatitis E virus 5 x 10 6, 5 x 10 5, 5 x 10 4, 5 x 10 3 copies/ml. Purified performance was analyzed by qrt-pcr detection. Ordering Info DESCRIPTION Cat.No. REACTION GMamp Virus DNA/RNA Kit GDP315 50 29 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Convenient: High-quality DNA and RNA from the same sample. Fast: The kit allows the parallel processing of multiple samples in 40~50 minutes. Safe: No phenol/chloroform extraction or ethaol precipitation. GMprep DNA/RNA Isolation Kit GMprep DNA/RNA Isolation Kit is designed for purifying both genomic DNA and total RNA from the same cell or tissue sample. The kit is compatible with a wide range of animal cells and tissues. A simple workflow allows the purification of both high-quality DNA and RNA from the same sample in only 40~50 minutes. The purified DNA and RNA are eluted separately and ready to use in any downstream application. Application RT-PCR, Real-time RT-PCR Ordering Info DESCRIPTION Cat.No. REACTION GMprep DNA/RNA Isolation Kit GDP422 50 GeneMark 30

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Convenient: Suitable for a wide range of sample types and sample sizes. Economic: One solution for purification of RNA, DNA and proteins. TriSolution Plus Reagent TriSolution Plus Reagent is a mixture of phenol, guanidium thiocynate, buffers and stabilizers developed for the simultaneously isolation of total RNA, DNA and proteins from animal and plant tissues, cells and bacteria culture. The entire procedure for total RNA isolation is an improved single-step method developed by Chomczynski and Sacchi. After homogenization of sample and chloroform extraction, three phases are formed (aqueous phase, interphase and organic phase). RNA can be precipitated by isopropanol from aqueous phase, DNA can be recovered by ethanol precipitation from interphase, and proteins are precipitated with isopropanol from organic phase. The reagent also includes a bottle of PS & PG Removal Solution, which can eliminate polysaccharides and proteoglycans contamination, by which the RNA can be dissolve easier and purer for RT-PCR and northern blot application. Application RNA isolated can be used for general molecular experiments such as Northern analysis, dot hybridization, poly A+ selection, in vitro translation, RNase protection assays, RT-PCR. 1 2 3 Figure1. RNA purified from rat kidney using TriSolution Plus Reagent. Total RNA was purified from rat kidney stored under different conditions. Lane 1: stored at 37 for 24 hr in RNAfter (GeneMark Cat.No.: RA100) Lane 2: stored at 4 for 30 days in RNAfter. Lane 3: stored at -70 for 30 days without RNAfter. Ordering Info DESCRIPTION Cat.No. SIZE TriSolution Plus Reagent TS-100-Plus 100 ml TriSolution Plus Reagent TS-200-Plus 200 ml 31 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Efficient: Performs well with small quantities of tissue (50~100 mg)and cells (5 10 6 ), and large quantities of tissue (>1 g) and cells (>10 7 ), of human, animals, plants, or bacterial origins. GRNzol Reagent GRNzol Reagent is a ready-to-use reagent for the purification of total RNA from cells and tissues. The reagent is a monophasic solution of phenol and guanidine isothiocyanate. During sample homogenization or lysis, GRNzol Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, DNA and proteins in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Total RNA isolated by GRNzol Reagent is free of protein and DNA contamination. Application Northern blot analysis, RT-PCR, dot blot hybridization, and molecular cloning. M 1 2 3 4 5 6 Sample Sample Size Yield Range Wheat 1 g 100~200 µg Mouse liver 1 mg 6~10 µg Cultured cells 10 6 5~10 µg E. coli 1 ml 2~10 µg Whole blood 1 ml 3~5 µg Figure 1. Total RNA extracted using GRNzol and related product from supplier A. Total RNA was extracted from 1 10 6 Human Jurkat cells. GRNzol yields a higher quantity of high-purity total RNA. Lane 1, 2, 3: RNA extracted using GRNzol Reagent Lane 4, 5, 6: RNA extracted using related product from supplier A. M: Marker Ordering Info DESCRIPTION Cat.No. SIZE GRNzol Reagent GDP405-Try 20 ml GRNzol Reagent GDP405-100 100 ml GRNzol Reagent GDP405-200 200 ml GeneMark 32

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Fast: RNA can be obtained within an hour. Yield: Higher RNA yields than GRNzol Reagent. GRNzol -A+ Reagent GRNzol A+ Reagent is designed in the base of GRNzol Reagent. The reagent is optimized for isolation of RNA from cell cultures and tissues. The GRNzol A+ Reagent is optimized for lysis of a variety of sample types before RNA isolation with standard homogenization methods and alcohol precipitation. This reagent performs well with small quantities of tissue (50~100 mg) and cells (5 10 6 ), and large quantities of tissue (>1 g) and cells (>10 7 ), of human, animals, plants, or bacterial origins. Application Northern blotting, qrt-pcr, poly(a) RNA selection, and RNase protection assays. Figure 1. Real-time PCR results of HPV virus RNA using GRNzol-A+ Reagent 10-fold serial dilutions of total HPV virus RNA (10 2 ~10 5 copies/ml) were quantified by qrt-pcr. Figure 2. Total RNA purified using GRNzol-A+ and related product from supplier A. Total RNA of higher quality was obtained using GRNzol-A+ Reagent. GRNzol-A+ Supllier A Ordering Info DESCRIPTION Cat.No. Size GRNzol-A+ Reagent GDP421-Try 20 ml GRNzol-A+ Reagent GDP421-100 100 ml GRNzol-A+ Reagent GDP421-200 200 ml 33 GeneMark

Molecular Biology Kits Molecular Biology Kits 96 well DNA & RNA Purification Kits High-throughput Rapid Economical High yield and high purity Ordering Info Cat. No. Package DP01-9602 DP01-9608 DP034-9602 DP034-9608 DP021-9602 DP021-9608 DP022-9602 DP022-9608 DP023-9602 DP023-9608 TR01-9602 TR01-9608 TR02-9602 TR02-9608 GM-96 Plasmid Miniprep Purification Kit GM-96 DNA Clean/Extraction Kit GM-96 Tissue & Cell Genomic DNA Purification Kit GM-96 Plant Genomic DNA Purification Kit GM-96 Blood Genomic DNA Purification Kit GM-96 Total RNA Purification Kit GM-96 Plant Total RNA Purification Kit 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well 2 x 96 well 8 x 96 well Cat. No. Package GM-WelVac-210 Vacuum Pump Set (without waste bottle) 1 Set GM-WelVac-210w Vacuum Pump Set (with waste bottle) 1 Set GM-Vac-400 Vacuum Manifold 1 Set GeneMark 34

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits GM-96 Plasmid Miniprep Purification Kit Features Fast: High throughput procedure, 96 wells at once. Yield: Obtain 8~10 μg of plasmid DNA from 2 ml culture per well. High Purity OD (260/280) > 1.8 per well. GM-96 Plasmid Miniprep Purification Kit provides a simple, fast, economic and automated high-throughput method to purify plasmid DNA from bacteria cultures. The procedure processes using both vacuum-driven transfer and centrifugation, and about 8 to10 μg plasmid DNA can be obtained from 2 ml culture for each well. This 96-well format kit is ideal for use in high-throughput automated sequencing projects. Application Purification of plasmid DNA from colonies. Purified plasmid DNA is suitable for subsequent applications such as cloning, enzyme digestion, ligation, transcription and sequencing. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 M 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 M 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Figure 1. Purification of puc18 using GM-96 Well Plasmid Miniprep Purification Kit. Lane M: GenKB LC DNA Ladder (GeneMark). Lane 1-96: Plasmid DNA purification from 96 x 2 ml culture of puc18 using GM-96 Well Plasmid Purification Kit. Figure 2. The sequencing data of plasmid DNA extracted using GM-96 Plasmid Miniprep Purification Kit. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Plasmid Miniprep Purification Kit DP01-9602 2 x 96 well GM-96 Plasmid Miniprep Purification Kit DP01-9608 8 x 96 well 35 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Fast: High throughput procedure, 96 wells at once. Capacity: 8~10 μg of DNA from 50 μl DNA sample for each well. GM-96 DNA Clean/Extraction Kit GM-96 DNA Clean/Extraction Kit provides a simple, efficient, and automated high- throughput method to purify DNA fragments from PCR products or other enzymatic reactions such as enzyme digestion, DNA ligation and probe labeling. The procedure processes using both vacuumdriven transfer and centrifugation, and can obtain 8 to10 μg DNA fragments from 50 μl DNA sample for each well. Application DNA clean-up from enzymatic reactions. Purified DNA is suitable for use in subsequent applications such as cloning, enzyme digestion, ligation, transcription and sequencing. M S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 M S 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 High Purity OD (260/280) > 1.8 per well. M S 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 M S 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 M S 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 M S 91 92 93 94 95 96 Figure 1. DNA clean up of puc18 plasmid using GM-96 DNA Clean Up Kit. Lane M: Gen100 DNA Ladder Lane 1-96: puc18 plasmid DNA was extracted using GM-96 Plasmid Purification Kit, and further purified using GM-96 DNA Clean/Extraction Kit to produce ultra pure DNA suitable for most subsequent applications. Figure 2. The sequencing data of DNA extracted by GM-96 PCR Clean-Up Kit. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 DNA Clean/Extraction Kit DP034-9602 2 x 96 well GM-96 DNA Clean/Extraction Kit DP034-9608 8 x 96 well GeneMark 36

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Fast: High throughput procedure, 96 wells at once. Yield: 8~16 μg of genomic DNA from 10 mg of tissue per well. High Purity OD (260/280) > 1.8 per well. GM-96 Tissue & Cell Genomic DNA Purification Kit GM-96 Tissue & Cell Genomic DNA Purification Kit provides a rapid, simple and effective approach to isolate the genomic DNA from various animal tissues, cultured cells and bacteria. The procedure processes using both vacuum-driven transfer and centrifugation, and yields 8 to16 μg genomic DNA from 10 mg of tissue for each well. Application Purified genomic DNA is suitable for use in subsequent applications such as PCR, Real-Time PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion and cloning. M 1 2 3 4 5 6 7 8 9 10 11 12 M 13 14 15 16 17 18 19 20 21 22 23 24 M 25 26 27 28 29 30 31 32 33 34 35 36 M 37 38 39 40 41 42 43 44 45 46 47 48 M 49 50 51 52 53 54 55 56 57 58 59 60 M 61 62 63 64 65 66 67 68 69 70 71 72 M 73 74 75 76 77 78 79 80 81 82 83 84 M 85 86 87 88 89 90 91 92 93 94 95 96 Figure 1. Purification of genomic DNA of Zebrafish tissue by GM-96 T & C Genomic DNA Purification Kit Lane M: GenKB LC DNA Ladder Lane 1-96: Genomic DNA purification from 96 x 10 mg of Zebrafish tissue using GM-96 T & C Genomic DNA Purification Kit. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Tissue & Cell Genomic DNA Purification Kit GM-96 Tissue & Cell Genomic DNA Purification Kit DP021-9602 DP021-9608 2 x 96 well 8 x 96 well 37 GeneMark

Molecular Biology Kits Molecular Biology Kits Features Fast: High throughput procedure, 96 wells at once. GM-96 Plant Genomic DNA Purification Kit GM-96 Plant Genomic DNA Purification Kit is designed for automated highthroughput and fast isolation of genomic DNA from various kinds of plants and fungi samples, especially for polysaccharide-rich plants and for plant tissues rich in secondary metabolites. The procedure processes using both vacuum-driven transfer and centrifugation, and yields 1.5 to 3 μg genomic DNA from 25 mg of tissue for each well. Application Purified DNA is suitable for use in subsequent applications such as PCR, Real- Time PCR, Southern blot, DNA hybridization, DNA sequencing, restriction digestion and cloning. Yield: 1.5~3 μg of genomic DNA from 25 mg of tissue per well. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 High Purity OD (260/280) > 1.8 per well. M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 M 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 M 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Figure 1.Purification of genomic DNA of rice leaf by GM-96 Plant genomic DNA Purification Kit Lane M: GenKB LC DNA Ladder Lane 1-96: Genomic DNA purification from 96 x 25 mg rice leaf tissue by GM-96 Plant Genomic DNA Purification Kit. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Plant Genomic DNA Purification Kit DP022-9602 2 x 96 well GM-96 Plant Genomic DNA Purification Kit DP022-9608 8 x 96 well GeneMark 38

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits GM-96 Blood Genomic DNA Purification Kit Features Fast: High throughput procedure, 96 wells at once. Yield: 1.5~3 μg of genomic DNA from 200 μl of blood per well. High Purity OD (260/280) > 1.8 per well. GM-96 Blood Genomic DNA Purification Kit is designed for automated highthroughput and fast isolation of genomic DNA from blood samples such as fresh or frozen whole blood and serum. The procedure processes using both vacuum-driven transfer and centrifugation, and 200 μl blood per well is sufficient to obtain 1.5 to 3 μg of blood genomic DNA. Application Purified DNA is suitable for use in subsequent applications such as PCR, Real- Time PCR, DNA sequencing, Southern blot, DNA hybridization, restriction digestion and cloning. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 M 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 M 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Figure 1. Purification of genomic DNA of human blood by GM-96 Blood Genomic DNA Purification Kit. Lane M: GenKB LC DNA Ladder Lane 1-96: Genomic DNA purification from 96 x 200 μl of human blood by GM-96 Blood Genomic DNA Purification Kit. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Blood Genomic DNA Purification Kit DP023-9602 2 x 96 well GM-96 Blood Genomic DNA Purification Kit DP023-9608 8 x 96 well 39 GeneMark

Molecular Biology Kits Molecular Biology Kits GM-96 Total RNA Purification Kit Features Fast: High throughput procedure, 96 wells at once. GM-96 Total RNA Purification Kit is designed for automated high-throughput isolation of total RNA from bacteria, viruses, animal tissues, cultured cells and various samples. The procedure processes using both vacuum-driven transfer and centrifugation, and 9 to 30 μg of total RNA can be obtained from each well depending on sample types. Application Purified RNA is suitable for use in subsequent applications such as RT-PCR, qrt-pcr, differential display, cdna synthesis and RNase protection assays. Yield: 9~30 μg of total RNA can be obtained from 1 ml sample per well. High Purity OD (260/280) > 1.8 per well. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 M 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 M 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 M 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 M 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 M 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 M 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 M 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 M 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 M 93 94 95 96 M 93 94 95 96 Figure 1. Total RNA purification from 1 ml of E. coli by GM-96 Total RNA Purification Kit. E.coli total RNA purified using GM-96 Total RNA Purification Kit was loaded and analyzed with 0.8 % agarose gel. Figure 2. RT-PCR test of total RNA purified using GM-96 Total RNA Purification kit. RT-PCR was performed for total RNA purified using GM-96 Total RNA Purification Kit. Figure indicates RNA quality suitable for most subsequent applications. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Total RNA Purification Kit TR01-9602 2 x 96 well GM-96 Total RNA Purification Kit TR01-9608 8 x 96 well GeneMark 40

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits GM-96 Plant Total RNA Purification Kit Features Fast: High throughput procedure, 96 wells at once. GM-96 Plant Total RNA Purification Kit is designed for automated highthroughput and fast isolation of total RNA from herbs, woody plants, succulent plants and fungi samples. The procedure processes using both vacuum-driven transfer and centrifugation, and 50 mg of plant tissue is sufficient to obtain 6 to 13 μg of plant total RNA from each well. Application Purified RNA is suitable for use in subsequent applications such as RT-PCR, qrt-pcr, differential display, cdna synthesis and RNase protection assays. Yield: 6~13 μg of plant total RNA can be obtained from 50 mg plant sample per well. High Purity OD (260/280) > 1.8 per well. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 M 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 M 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 M 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 M 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 M 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 M 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 M 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 M 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Figure 1. Plant total RNA purification from 50 mg of rice leaf by GM-96 Plant Total RNA Purification kit. Rice total RNA was purified using GM-96 Plant Total RNA Purification Kit and analyzed with 0.8 % agarose gel. Figure 2. RT-PCR test of plant total RNA purified using GM-96 Plant Total RNA Purification kit. RT-PCR was performed for rice total RNA purified using GM-96 Plant Total RNA Purification kit. Figure indicated RNA quality suitable for most subsequent applications. Ordering Info DESCRIPTION Cat.No. PACKAGE GM-96 Plant Total RNA Purification Kit TR02-9602 2 x 96 well GM-96 Plant Total RNA Purification Kit TR02-9608 8 x 96 well 41 GeneMark

Molecular Biology Kits Molecular Biology Kits Protein Quantitation Assay Kits High sensitivity Fast Available for wide range of protein concentration Ordering Info Product Cat. No. Protein concentration Range Bradford Assay Kit BCA Protein Assay Kit GPA102-0500 GPA102-1000 GPA115-050 GPA115-250 50~1,000 µg/ml 20~2,000 µg/ml GeneMark 42

GeneMark GMbiolab Co., Ltd. Molecular Biology Kits Molecular Biology Kits Features Convenient: Ready-to-use dye-binding reagent formula Sensitivity : Fast color development; measured at 595 nm. Wide range: Useful for determining protein concentration from 50~1,000 µg/ml. Protein Quantitation Assay Kit Bradford Assay Kit The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard Reagent. The simple procedure is adaptable to nearly any volume scale, including test tubes and microplates. Binding of protein to Coomassie dye under acidic environment of the reagent results in spectral shift from reddish/brown form of dye (absorbance maximum at 465 nm) to the blue form of dye (absorbance maximum at 610 nm). The difference between the two forms of the dye is greatest at 595 nm, which is the optimal wavelength to measure the blue color from the Coomassie dye-protein complex. Application Determination of protein concentration Ordering Info DESCRIPTION Cat.No. Size Bradford Assay Kit Bradford Assay Kit GPA102-0500 GPA102-1000 165 preps (tube test)/825 preps (microplate test), 500 ml 330 preps (tube test)/1650 preps (microplate test),1000 ml Features Sensitivity : Fast color development; measured at 562 nm. Wide range: Useful for determining protein concentration from 20~2,000 µg/ml. BCA Protein Assay Kit BCA Protein Assay Kit provides a convenient format for stable and sensitive detection and quantitation of total protein. This method combines the wellknown reduction of Cu 2+ to Cu + by protein in an alkaline medium (the biuret reaction) with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu + ) using a unique reagent containing bicin-choninic acid. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA to one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is almost linear with increasing protein concentrations. This kit contains ready-to-use coomassie G-250 dye and bovine serum albumin to be used as protein standards. Application Determination of protein concentration Ordering Info DESCRIPTION Cat.No. Size BCA Protein Assay Kit GPA115-050 50 preps (tube)/500 preps (board) BCA Protein Assay Kit GPA115-250 250 preps (tube)/2500 preps (board) 43 GeneMark

PCR Related Product PCR Related Product A. PCR Polymerases.....45 B. PCR Master Mix...51 C. GM Real-Time qpcr Kit.60 D. One-Step & Two-Step RT-PCR Kits.62 E. PCR Cloning Kits & DNA Ligation Kit..69 GeneMark 44

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product PCR Polymerases Product Name GenTaq DNA Polymerase GenTaq Plus DNA Polymerase Cloned Pfu DNA Polymerase GenHot Taq DNA Polymerase etaq DNA Polymerase Polymerase GenTaq GenTaq + Pfu Cloned Pfu Taq + Mab ExtraTaq Proofreading No Yes Yes No No Product Overhang 3 -A 3 -A blunt 3 -A 3 -A PCR product size Up to 6 kb Up to 14 kb Up to 3 kb Up to 6 kb Up to 6 KB Application Detection Detection Cloning Expression Cloning Expression Mutagenesis Detection Multiplex PCR qpcr High Efficiency Detection Ordering Info Product Name Cat # PCR Polymerase GenTaq DNA Polymerase GenTaq Plus DNA Polymerase Pfu DNA Polymerase GenHot Taq DNA Polymerase etaq DNA Polymerase GMright HF DNA Polymerase GM008 GM008P D0090P GM008HS GM008E GHF-1000 45 GeneMark

PCR Related Product PCR Related Product GenTaq DNA Polymerase Features Thermostable: Is stable during prolonged incubation at high temperatures. GenTaq DNA polymerase is a thermostable DNA polymerase isolated and purified from E.coli strain that carries a plasmid with cloned Taq DNA polymerase. It lacks 3 to 5' proofreading activity but possess a 5 to 3' exonuclease activity in the same polypeptide as the DNA polymerase. Application GenTaq is designed for use in DNA amplification, primer extension reaction and DNA sequencing. Primer Extension Characteristics: GenTaq has the templateindependent terminal transferase activity which results in the addition of a single nucleotide (adenosine) at 3 end of extension product. So TA cloning vector is recommended if the extension product is needed to be cloned. M 1 2 3 M 4 5 6 M 7 8 9 Figure 1. Amplification of different sized PCR products using GenTaq DNA Polymerase (5U/μl) Lane M: Gen100 DNA Ladder or GenKB DNA Ladder Lane1-3: Amplified 500 bp fragment with GenTaq Lane4-6: Amplified 3.9 kb fragment with GenTaq Lane7-9: Amplified 6.0 kb fragment with GenTaq - 6 kb - 3.9 kb - 500 bp Ordering Info DESCRIPTION Cat.No. Size GenTaq DNA Polymerase GM008-2-250 (2U/μl) 250 U GenTaq DNA Polymerase GM008-2-500 (2U/μl) 500 U GenTaq DNA Polymerase GM008-2-1000 (2U/μl) 1000 U GenTaq DNA Polymerase GM008-5-250 (5U/μl) 250 U GenTaq DNA Polymerase GM008-5-500 (5U/μl) 500 U GenTaq DNA Polymerase GM008-5-1000 (5U/μl) 1000 U GeneMark 46

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product GenTaq Plus DNA Polymerase Features Thermostable: Is stable during prolonged incubation at high temperatures. Efficiency and Fidelity: Provides more efficient amplification and higher fidelity (six-fold increase in fidelity over Taq DNA polymerase alone). GenTaq Plus is an enzyme mixture of GenTaq (GM008) and pfu (D0090P) DNA polymerase, it provides more efficient amplification and higher fidelity than conventional Taq DNA polymerase under conventional PCR conditions. In optimized PCR, GenTaq Plus DNA Polymerase can result in a six-fold increase in fidelity over Taq DNA polymerase alone and is effective over a wide range of target sizes up to 14 kb with some optimization. GenTaq Plus includes two buffers, one GenTaq Buffer for PCR product smaller than 6 kb with high fidelity, and one PC2 Buffer for PCR product larger than 6 kb. Application GenTaq Plus can be used for DNA amplification, primer extension reaction and DNA sequencing. Secondary structure may be decreased in polymerization at high temperature. Effectiveness: Effective over a wide range of target sizes up to 14 kb. Characteristics: Mixture of GenTaq and pfu DNA polymerase. M 6kb 8kb 14kb Figure 1. Products were amplified using GenTaq Plus DNA polymerase GenTaq Plus DNA polymerase can amplify products up to 14 kb in length. Ordering Info DESCRIPTION Cat.No. SIZE GenTaq Plus DNA Polymerase GM008P (5U/μl) 250 U GenTaq Plus DNA Polymerase GM008P-1000 (5U/μl) 1000 U 47 GeneMark

PCR Related Product PCR Related Product Pfu DNA Polymerase Features Efficiency and Fidelity: Twelve-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. Stability: Stable within 12 months after receiving. Pfu DNA polymerase is isolated from Pyrococcus furiosus. The multi functional thermostable enzyme possesses both 5 to 3 DNA polymerase and 3 to 5 exonuclease activity, which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerases. Pfu DNA polymerase has a temperature optimum between 72 ~78, and remains more than 95% activity following one hour incubation at 95. As Pfu DNA Polymerase possesses both of 5 to 3 DNA polymerase and 3 to 5 DNA exonuclease activity, it results in blunt ends of DNA synthesis. Application Pfu DNA polymerase can be used for DNA amplification, primer extension reaction and DNA sequencing. Secondary structure may be decreased in polymerization at high temperature. Primer Extension Characteristics: As Pfu DNA Polymerase possesses both 5 to 3 -DNA polymerase and 3 to 5 - exonuclease activity, it results in blunt ends of DNA synthesis. M 3kb 1kb M 1kb Figure 1. Gel electrophoresis analysis of PCR product generated using Pfu DNA polymerase. Pfu DNA polymerase can amplify products up to 3 kb in length. M: Gen100 DNA Ladder Figure 2. Gel electrophoresis analysis of Enterobacteria phage lambda ea10 gene amplification using Pfu DNA polymerase. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. Size Pfu DNA Polymerase D0090P-250 (5 U/μl) 250 U Pfu DNA Polymerase D0090P-500 (5 U/μl) 500 U GeneMark 48

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product GenHot Taq DNA Polymerase Features Characteristics: Heat-mediated activation. Specificity: It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to standard DNA polymerases. GenHot Taq DNA Polymerase is a complex mixture of a thermostable 94 kd Taq DNA Polymerase purified from E.coli recombinant strain expressing Thermus aquatiqus polymerase gene and specific monoclonal antibodies. GenHot Taq DNA Polymerase is inactive under conditions of amplification reaction preparation. An advantage of GenHot Taq DNA Polymerase is the absence of additional heating step for polymerase activation. Heat activation of enzyme occurs during the first denaturation step. An inactive GenHot Taq DNA Polymerase-antibody complex dissociates automatically when temperature reaches 63, allowing activation of DNA polymerase. Application GenHot Taq DNA polymerase can be used for DNA segment amplification, multiplex PCR, Real-Time PCR, and in high sensitivity applications when high specificity is required. M Taq GenHot Taq non-specific band Figure 1. Gel electrophoresis analysis of PCR products generated using Taq and GenHot Taq polymerases GeneMark Hot start Taq DNA polymerase prevents amplification of non-specific products to provide increased efficiency. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. SIZE GenHot Taq DNA Polymerase GM008HS-200 (5 U/μl) 200 U GenHot Taq DNA Polymerase GM008HS-1000 (5 U/μl) 1000 U 49 GeneMark

PCR Related Product PCR Related Product etaq DNA Polymerase etaq (Extra Taq DNA Polymerase) is especially designed for PCR amplification of low concentration templates. This DNA polymerase is thermostable, and is purified from E.Coli strain carrying plasmid with cloned Taq DNA polymerase gene. It lacks 3 to 5 proofreading activity, but possess a 5 to 3 exonuclease activity. With the least amount of enzyme, for higher amplification efficiency. Features Characteristics: Generates PCR products with 3 -A. Application etaq DNA polymerase can be used for performing PCR amplification, primer extension reaction, and DNA sequencing. Especially suitable for samples where the gdna is difficult to extract (eg. With low copies). Efficiency: 0.1 U is sufficient for amplification of 1 ng gdna templates. Especially suitable for samples where thr gdna is difficult to extract, or when the gdna template is with low copies. A. Plasmid 1 U 0.5 U 0.25 U 0.1 U M 10 1 10 1 10 1 10 1 (ng) B. Bacteria 1 U 0.5 U 0.25 U 0.1 U M 100 10 1 100 10 1 100 10 1 100 10 1 (ng) C. Plant 1 U 0.5 U 0.25 U 0.1 U M 100 10 1 100 10 1 100 10 1 100 10 1 (ng) D. Animal tissue 1 U 0.5 U 0.25 U 0.1 U M 100 10 1 100 10 1 100 10 1 100 10 1 (ng) Fig 1. Amplification of various samples at difference enzyme units and sample concentrations. (A):Enterobacteria phage lambda Plasmid of 1 ng and 10 ng is used as templates, and ea59 gene (2934 bp) was amplified using 0.1 U, 0.25 U, 0.5 U and 1U of etaq Extra Taq DNA polymerase. (B), (C)&(D): E. Coli, rice and zebrafish genomic DNA of 1 ng, 10 ng and 100 ng is used as templates, and fruk gene (963 bp), actin gene (406 bp) and β actin gene (550 bp) were amplified using 0.1 U, 0.25 U, 0.5 U and 1U of etaq Extra Taq DNA polymerase, respectively. M:GenKB DNA Ladder Ordering Info DESCRIPTION Cat.No. Size etaq Extra Taq DNA Polymerase GM008E-250 250 U etaq Extra Taq DNA Polymerase GM008E-500 500 U etaq Extra Taq DNA Polymerase GM008E-1000 1000 U GeneMark 50

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product PCR Master Mix Product Name PCR Master Mix II PCR Plus Master Mix II PCR Pfu Master Mix II PCR Hot-Start Master Mix II Polymerase GenTaq GenTaq + Pfu Cloned Pfu Taq + Mab Proofreading No Yes Yes No Product Overhang 3 -A 3 -A blunt 3 -A PCR product size Up to 6 kb Up to 14 kb Up to 3 kb Up to 6 kb Application Detection Detection Cloning Expression Cloning Expression Mutagenesis Detection Multiplex PCR qpcr Ordering Info Product Name Cat # PCR Master Mix II 5X RP02-II PCR Plus Master Mix II 5X RP02Plus-II PCR Master Mix PCR Pfu Master Mix II 5X RP02Pfu-II PCR Hot-Start Master Mix II 5X RP02HS-II etaq Master Mix 5X RP02E GMright HF DNA Polymerase Premix 2X GHF-M1000 GMmulti HS Taq Premix 2X GMH-M7100 PCR Dye Master Mix PCR Dye Master Mix II 2X 5X RP02D-II-B RP02D-II-A PCR Dye Plus Master Mix 5X RP02D-Plus PCR Dye Pfu Master Mix 5X RP02D-Pfu PCR Dye Hot-Start Master Mix 5X RP02D-HS 51 GeneMark

PCR Related Product PCR Related Product PCR Master Mix II (5X) The PCR Master Mix II is a ready-to-use mixture for all PCR applications. The mix contains all components required for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Features Convenience : Time saving and ready to use for PCR reaction. Components: Supplied as a 5X concentrated ready-to-use mix. Each of the resulting 1X PCR reaction contains 0.75 U Taq DNA polymerase, reaction buffer, 2 mm MgCl 2, 250 μm dntp and enzyme stabilizer. Application DNA amplification, primer extension reaction and DNA sequencing. 6 kb 2 kb Figure1. DNA amplification using GeneMark PCR Master Mix II DNA fragments with the length of 2 kb and 6 kb were amplified using 5X PCR Master Mix II. M: GenKB DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION PCR Master Mix II (5X) RP02-II-400 400 PCR Master Mix II (5X) RP02-II-2000 2000 GeneMark 52

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product Features Convenience : Time saving and ready to use for PCR reaction. Characteristics: High sensitivity and reduces contamination. Proof-reading, suitable for DNA cloning Components: Supplied as a 5X concentrated ready-to-use mix. Each of the resulting 1X PCR reaction contains 1.5 U of GenTaq Plus polymerase, reaction buffer, MgCl 2 (final concentration 2 mm), 250 μm dntp and enzyme stabilizer. PCR Plus Master Mix II (5X) The PCR Plus Master Mix II is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR applications. The mix contains GenTaq Plus, which is an enzyme mixture of GenTaq and Pfu DNA polymerase. It has high fidelity with an error frequency 1.6/10 6 during DNA synthesis and is effective over a wide range of target sizes up to 14 kb with some optimization. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Application DNA amplification, primer extension reaction and is suitable for DNA cloning. M NinB Xis Ordering Info 6 kb 2 kb Figure 1. Amplification of Enterobacteria phage lambda gene fragments using GeneMark PCR Plus Master Mix II. NinB (2 kb) and xis (6 kb) genes from Enterobacteria phage lambda are amplified and analyzied with agarose gel. M: GenKB DNA Ladder DESCRIPTION Cat.No. REACTION PCR Plus Master Mix II (5X) RP02Plus-II 50 53 GeneMark

PCR Related Product PCR Related Product Features Convenience : Time saving and ready to use for PCR reaction, reduces contamination. Characteristics: Thermostable enzyme that possesses both 5 to 3 DNA polymerase and 3 to 5 exonuclease activity. 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. Components: Supplied as a 5X concentrated ready-to-use mix. Each of the resulting 1X PCR reaction contains 1.5 U of Pfu DNA polymerase, reaction buffer, MgSO 4 (final concentration 2 mm), 250 μm dntp and enzyme stabilizer. PCR Pfu Master Mix II (5X) The PCR Pfu Master Mix II is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. Pfu DNA polymerase is isolated from the Pyrococus furiosus. The multifunctional thermostable enzyme possesses both of 5 to 3 DNA polymerase and 3 to 5 exonuclease activity which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. The mix contains Pfu DNA polymerase and other components required for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Application DNA amplification, primer extension reaction and DNA sequencing. M Ordering Info 3 kb Firgure 1. Amplification of ea59 gene of Enterobacteria phage lambda using PCR Pfu Master Mix II. Pfu Taq polymerase possesses high fidelity of DNA synthesis. No nonspecific amplification observed for ea59 gene fragment amplification. M: GenKB DNA Ladder DESCRIPTION Cat.No. REACTION PCR Pfu Master Mix II (5X) RP02Pfu-II 100 GeneMark 54

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product Features Convenience : Time saving and ready to use for PCR reaction. PCR Hot-Start Master Mix II (5X) The PCR Hot-Start Master Mix II is a ready-to-use mixture for virtually all PCR applications. Hot Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during pre-amplification stage and thus may provide improved specificity when compared to standard DNA polymerase. The mix contains Hot-Start Taq DNA polymerase and other components required for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Application DNA amplification, primer extension reaction and DNA synthesis. Characteristics: High sensitivity and reduces contamination. Thermostable and prevents amplification of non-specific priming products. M 2 kb Non-specific product Components: Supplied as a 5X concentrated ready-to-use mix. Each of the resulting 1X PCR reaction contains 0.75 U of Hot Start Taq DNA polymerase, reaction buffer, MgCl 2 (final concentration 2 mm), 250 μm dntp and enzyme stabilizer. Figure1. Amplification of NinB gene of Enterobacteria phage lambda using PCR Hot-Start Master Mix. Comparison of PCR amplification using PCR Master Mix and Hot-Start PCR Master Mix. Hot-Start Taq polymerase is activated under specific temperature condition and thus eliminates undesired amplification. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION PCR Hot-Start Master Mix II (5X) RP02HS-II 200 55 GeneMark

PCR Related Product PCR Related Product PCR Dye Master Mix II (2X/5X) The 2X or 5X PCR Dye Master Mix II is a ready-to-use mixture for virtually all PCR applications. The mixes contain all components for PCR with exception of template and primers. A red dye contained in the mix allows the PCR product to be directly loaded onto the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Features Convenience : Time saving and ready to use for PCR reaction. Components: The 2X or 5X PCR Dye Master Mix II is supplied as a 2X or 5X concentrated ready-touse mix, relatively. Each of the resulting 1X PCR reaction contains 0.75 U of Taq DNA polymerase, reaction buffer, 2 mm MgCl 2, 250 μm dntp and enzyme stabilizer. Application DNA amplification, primer extension reaction and DNA synthesis. M NinB xis 6 kb 2 kb Figure 1. Amplification of Enterobactria phage lambda NinB gene and xis gene using GeneMark PCR Dye Master Mix II Gel analysis of amplified Enterobacteria phage lambda NinB gene (2 kb) and xis gene (6 kb). PCR product is directly loaded onto agarose gel. M: GenKB DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION 5X PCR Dye Master Mix II RP02D-II-A200 200 5X PCR Dye Master Mix II RP02D-II-A1000 1000 2X PCR Dye Master Mix II RP02D-II-B200 200 2X PCR Dye Master Mix II RP02D-II-B1000 1000 GeneMark 56

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product PCR Dye Plus Master Mix (5X) Features Convenience : Time saving and ready to use for PCR reaction. Components: Supplied as a 5X concentrated ready-to-use mix. Each of the resulting 1X PCR reaction contains 1.5 U of GenTaq Plus polymerase, reaction buffer, MgCl 2 (final concentration 2 mm), 250 μm dntp and enzyme stabilizer. The PCR Dye Plus Master Mix is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR applications. The mix contains GenTaq Plus, which is an enzyme mixture of GenTaq and Pfu DNA polymerase, it has high fidelity with an error frequency 1.6/10 6 during DNA synthesis and is effective over a wide range of target sizes up to 14 kb with some optimization. A red dye is also contained in the mix allowing the PCR products to be directly loaded onto the gel. Application DNA amplification, primer extension reaction and DNA synthesis. M 6 kb 3 kb Figure 1. Amplification of Enterobactria phage lambda ea59 gene and xis gene using GeneMark PCR Dye Plus Master Mix. Gel analysis of amplified Enterobacteria phage lambda ea59 gene (3 kb) and xis gene (6 kb). PCR product is directly loaded onto agarose gel. M: GenKB DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION PCR Dye Plus Master Mix (5X) RP02D-Plus 100 57 GeneMark

PCR Related Product PCR Related Product Features Convenience : Time saving and ready to use for PCR reaction. Characteristics: Thermostable enzyme that possesses both of 5 to 3 DNA polymerase and 3 to 5 exonuclease activity. PCR Dye Pfu Master Mix (5X) The PCR Dye Pfu Master Mix is a ready-to-use mixture for amplification of specific DNA fragments. It can be used in place of the single reagent for virtually all PCR application. The mix contains Pfu DNA polymerase and other components required for PCR with exception of template and primers. A red dye contained in the mix allows PCR products to be directly loaded onto the gel. Pfu DNA polymerase is isolated from the Pyrococus furiosus. The multifunctional thermostable enzyme possesses both of 5 to 3 DNA polymerase and 3 to 5 exonuclease activity which results in a 12-fold increase in fidelity of DNA synthesis over Taq DNA polymerase. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Application DNA amplification, primer extension reaction and DNA synthesis. M Components: Supplied as a 5X concentrated ready-touse mix. Each of the resulting 1X PCR reaction contains 1.5 U of Pfu DNA polymerase, reaction buffer, MgSO 4 (final concentration 2 mm), 250 μm dntp and enzyme stabilize. 3 kb 1 kb Figure 1. Amplification of Enterobactria phage lambda ea59 gene and ea10 gene using GeneMark PCR Dye Pfu Master Mix Gel analysis of amplified Enterobacteria phage lambda ea59 gene (3 kb) and ea10 gene (1 kb). PCR product is directly loaded onto agarose gel. M: GenKB DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION PCR Dye Pfu Master Mix (5X) RP02D-Pfu 100 GeneMark 58

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product PCR Dye Hot-Start Master Mix (5X) Features Convenience : Time saving and ready to use for PCR reaction. Characteristics: High sensitivity and reduces contamination. Thermostable and prevents amplification of non-specific priming products. The PCR Dye Hot-Start Master Mix is a ready-to-use mixture for virtually all PCR applications. Hot-Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mispriming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. The mix contains Hot-Start Taq DNA polymerase and other components required for PCR with exception of template and primers. A red dye contained in the mix allows PCR products to be directly loaded onto the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. Application DNA amplification, primer extension reaction and DNA synthesis. M PCR Dye Master Mix II Hot-Start Dye Master Mix Components: Supplied as a 5X concentrated ready-touse mix. Each of the resulting 1X PCR reaction contains 0.75 U of Hot- Start Taq DNA polymerase, reaction buffer, 2 mm MgCl 2, 250 μm dntp and enzyme stabilizer. Ordering Info 2 kb Non-specific bands Figure 1. Comparison of DNA amplification of Enterobactria phage lambda NinB gene using PCR Dye Master Mix II and PCR Dye Hot-Start Master Mix. Gel electrophoresis analysis of amplified Enterobacteria phage lambda NinB gene. Non-specific bands are not observed for PCR products amplified using Hot-Start Dye Master Mix II. M: Gen100 DNA Ladder DESCRIPTION Cat.No. REACTION PCR Dye Hot Start Master Mix (5X) RP02D-HS 200 59 GeneMark

PCR Related Product PCR Related Product GM Real-Time qpcr Kits (SYBR green/probe systems) Products DESCRIPTION Cat.No. Size GM SYBR qpcr Kit QPSY01 1 ml, 5 ml, 25 ml, 100 ml GM SYBR qpcr Kit (w/o ROX) QPSY02 1 ml, 5 ml, 25 ml, 100 ml GMperfect SYBR q-pcr Mix (w/ ROX) QPSY03 1 ml, 5 ml, 25 ml, 100 ml GMperfect HS-qPCR Mix (For Probe) QP7100N 1 ml, 5 ml, 25 ml, 100 ml GeneMark 60

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product Features Characteristics: Quick and accurate detection and quantification of target gene. High amplification efficiency and highly sensitive detection. 2X GM SYBR qpcr Kit GM SYBR qpcr Kit is supplied as a ready-to-use mix containing all components required for amplification and detection of DNA in qpcr with exception of primers and template. The GM SYBR qpcr Kit is supplied as a 2X Master Mix with integrated antibody-mediated hot start Taq, SYBR Green I fluorescent dye, MgCl 2, dntps, ROX reference dye and stabilizers. GM SYBR qpcr Kit is intended for use on all real-time instruments and protocols. Application Real-time PCR/qPCR, gene expression analysis and genotyping. Sample conc. (ng) GM Cт Supplier K 125 24.0 25.0 62.5 25.0 26.3 31.5 26.1 27.5 15.62 27.0 28.2 7.81 28.0 29.6 Figure 1. Performance comparison of GM SYBR qpcr Kit and supplier K's master mix. GM Supplier K slope -3.354-3.639 R 2 0.999 0.993 PCR Eff. 98.667% 88.272% A 120 bp fragment of the Zebrafish betaactin gene was amplified from a set of five 2-fold dilutions of Zebrafish genomic DNA (125 ng to 7.8 ng) using GM SYBR qpcr Master Mix (blue) and qpcr kit from supplier K (red). Reactions were performed according to the protocol: 1 cycle at 95 for 5 min, followed by 35 cycles at 95 for 15 sec, 60 for 50 sec on an ABI-StepOne. Ordering Info DESCRIPTION Cat.No. SIZE GM SYBR qpcr Kit QPSY01 1 ml x 1 GM SYBR qpcr Kit QPSY01-5 1 ml x 5 GM SYBR qpcr Kit QPSY01-25 1 ml x 25 GM SYBR qpcr Kit QPSY01-100 1 ml x 100 61 GeneMark

PCR Related Product PCR Related Product RT Kits and One-Step & Two-Step RT-PCR Kits RT kits GMsensitive RT Mix (2X) (without Oligo (dt)) SuperSAMscript RT DryMix (2X) (without Oligo (dt)) Cat. No. GSR-M1000 GRT-M002 One-Step RT-PCR kits One-Step RT-PCR Kit (MMLV/GenTaq) One-Step RT-PCR Plus Kit (MMLV/GenTaq Plus) One-Step Pfu RT-PCR Kit (MMLV/Pfu) One-Step HotStart RT-PCR Kit (MMLV/GenHot Start Taq) One-Step RT-PCR Kit (AMV/GenTaq) One-Step RT-PCR Plus Kit (AMV/GenTaq Plus) One-Step Pfu RT-PCR Kit (AMV/Pfu) One-Step HotStart RT-PCR Kit (AMV/GenHot Start Taq) GM One-Step qrt-pcr Kit (SYBR Green) GMsensitive One-Step RT-PCR Mix (2X) (without Oligo(dT)) Cat. No. RP01 RP01-Plus RP01-Pfu RP01-HS RP01/A RP01-Plus/A RP01-Pfu/A RP01-GH/A RPQ01 GSRP-M1000 Two-Step RT-PCR kits Two-Step MMLV RT-PCR Kit (MMLV/GenTaq) Two-Step MMLV Plus RT-PCR Kit (MMLV/GenTaq Plus) Two-Step MMLV Pfu RT-PCR Kit (MMLV/Pfu) Two-Step MMLV HotStart RT-PCR Kit (MMLV/GenHot Start Taq) Two-Step AMV RT-PCR Kit (AMV/GenTaq) Two-Step AMV Plus RT-PCR Kit (AMV/GenTaq Plus) Two-Step AMV Pfu RT-PCR Kit (AMV/Pfu) Two-Step AMV HotStart RT-PCR Kit (AMV/GenHot Start Taq) Cat. No. RP012-M RP012-Plus/M RP012-Pfu/M RP012-GH/M RP012-A RP012-Plus/A RP012-Pfu/A RP012-GH/A GeneMark 62

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product One-Step RT-PCR Kit (MMLV/GenTaq) Features Convenience : One-tube, two-enzyme system, easy one-tube reaction. Characteristics: Unique blend of MMLV RT and Taq DNA polymerase with high sensitivity. The One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mrna. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNA. The system uses M-MLV reverse transcriptase for first-strand cdna synthesis and GenTaq DNA polymerase for PCR amplification. The amplified DNA fragments are generated with a 3 A- overhang that can be cloned into T-vector. M-MLV reverse transcriptase lacks a 3 to 5 exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cdna amplification. Application Reverse transcription of total RNA or mrna, followed by cdna PCR amplification. brain kidney liver spleen brain kidney liver spleen brain kidney liver spleen Useful for full-length cdna amplification. One-step RT-PCR for any RNA templates. Optimized buffer for efficient reverse transcription and amplification. Figure 1. Total RNA 200 pg each from mouse brain, kidney, liver and spleen were used to perform one step RT-PCR. Lane 1-4 : actin RT-PCR products Lane 5-8 : clathrin RT-PCR products Lane 9-12: GAPDH RT-PCR products Copies of RNA target M 10 6 10 5 10 4 10 3 10 2 10 1 320 bp Figure 2. Sensitivity of One Step RT-PCR kit. Amplification of specific RNA from serial dilution of RNA templates with One-Step RT PCR kit, RNA templates were prepared by in vitro transcription and copies numbers were determined and calculated by spectrophotometer and gel phosphor screen imaging. Ordering Info DESCRIPTION Cat.No. REACTION One-Step RT-PCR kit RP01 50 One-Step RT-PCR kit RP01-250 250 63 GeneMark

PCR Related Product PCR Related Product Features Convenience : One-tube, two-enzyme system, easy one-tube reaction. Characteristics: Useful for full-length cdna amplification. One-step RT-PCR for any RNA template. Optimized buffer for efficient reverse transcription and amplification. High fidelity: Error frequency 1.6/10 6 during DNA synthesis. One-Step RT-PCR Plus Kit (MMLV/GenTaq Plus) The One-Step RT-PCR Plus Kit is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mrna. This onetube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNA. The system uses M-MLV reverse transcriptase for first-strand cdna synthesis and GenTaq Plus for PCR amplification. M-MLV reverse transcriptase lacks a 3 to 5 exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cdna amplification. The kit contains GenTaq Plus, which is an enzyme mixture of GenTaq and Pfu DNA polymerase. It has high fidelity with an error frequency 1.6/10 6 during DNA synthesis, and the PCR product can be directly used for cloning to TA or expressional vectors. Application Reverse transcription of total RNA or mrna, followed by cdna PCR amplification. M Molecules of RNA per reaction 300 bp Figure1. Gel electrophoresis analysis of RT-PCR product generated using One-step RT-PCR Plus (MMLV/GenTaq Plus) kit. Different concentration of RT-PCR products generated using E.coli total RNA as template is loaded and analyzed. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION One-Step RT-PCR Plus kit RP01-Plus 50 One-Step Pfu RT-PCR Kit (MMLV/Pfu) Application Reverse transcription of total RNA or mrna, followed by cdna PCR amplification. Ordering Info DESCRIPTION Cat.No. REACTION One-Step Pfu RT-PCR kit RP01-Pfu 50 GeneMark 64

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product One-Step Hot Start RT-PCR Kit (MMLV/GenHot Start Taq) Features Convenience : One-tube, two-enzyme system, easy one-tube reaction. Characteristics: Useful for full-length cdna amplification. One-step RT-PCR for any RNA template. The One-Step Hot-Start RT-PCR Kit is designed for reverse transcription and PCR amplification of specific target RNA from either total RNA or mrna. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNA. The system use M-MLV reverse transcriptase for firststrand cdna synthesis and Hot Start Taq DNA polymerase for PCR amplification. M-MLV reverse transcriptase lacks a 3 to 5 exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cdna amplification. The kit contains GenTaq Hot Start DNA polymerase, which is a complex mixture of a thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during pre-amplification stage. Application Reverse transcription of total RNA or mrna, followed by cdna PCR amplification. Sensitivity: Thermostable Taq DNA polymerase and specific monoclonal antibodies. M Molecules of RNA per reaction Eliminates primer-dimer formation and mis-priming. 300 bp Figure1. Gel electrophoresis analysis of RT-PCR product generated using One-step RT-PCR (MMLV/GenHot Start Taq) kit. Different concentrations of RT-PCR products generated using E.coli total RNA as template is loaded and analyzed. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION One-Step Hot-Start RT-PCR kit RP01-HS 50 65 GeneMark

PCR Related Product PCR Related Product One-Step RT-PCR Kit (AMV Reverse Transcriptase Series) Features Characteristics: AMV reverse transcriptase possesses 5 to 3 DNA polymerase activity. Possess RNase H activity that breaks apart RNA:DNA hybrids. Effective amplification up to 6 kb DNA. The One-Step RT-PCR Kit (AMV Reverse Transcriiptase Series) is designed for reverse transcription and PCR amplification of specific target RNA from either total RNA or mrna. This system provides sensitive, quick and reproducible analysis of even rare RNA. AMV reverse transcriptase is used for first-strand cdna synthesis, in combination with either GenTaq DNA polymerase, GenTaq Plus DNA polymerase, Pfu DNApolymerase or GenHot Taq DNA polymerases for PCR amplification. Application Reverse transcription of total RNA or mrna, followed by cdna PCR amplification. Ordering Info DESCRIPTION Cat.No. REACTION One-Step RT-PCR kit (AMV/GenTaq) RP01-A 50 One-Step RT-PCR Plus kit (AMV/GenTaq Plus) RP01-Plus-A 50 One-Step RT-PCR Pfu kit (AMV/Pfu) RP01-Pfu-A 50 One-Step RT-PCR kit (AMV/GenHot Start Taq) RP01-GH-A 50 GeneMark 66

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product Two-Step MMLV RT-PCR Kit Features Characteristics: Effective amplification of DNA up to 6 kb in length. Sensitivity: Eliminates primer-dimer formation and mis-priming. Components: Supplied as a 5X concentrated ready-to-use mix, that is, a 1X mixture of recombinant 1.5 U of Hot Start Taq DNA polymerase, reaction buffer, 2 mm MgCl 2, 250 μm dntp and enzyme stabilizer sufficient to allow efficient amplification of template in 50 μl PCR. The GeneMark Two-Step M-MuLV RT-PCR Kit Series contains all the components required to perform first-strand cdna synthesis and second strand DNA amplification. Moloney Murine Leukemia Virus Reverse Transcriptase (M- MuLV RTase) is a frequent choice for complementary DNA (cdna) synthesis because of its ease of use and low intrinsic RNase activity. In the cdna synthesis step, RNA is reverse transcribed by MMLV RTase to produce its cdna. For the subsequent amplification of the cdna template, PCR Hot-Start Master Mix is provided. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during pre-amplification stage and thus may provide improved specificity. PCR Hot-Start Master Mix II can be used for effective amplification of DNA up to 6 kb in length. Application Second strand cdna synthesis, PCR and cloning. M fruk 1 kb Figure1. RT-PCR result of E.coli (DH5α) total RNA with amplification of second- strand cdna fruk gene. GeneMark Two-Step MMLV RT-PCR Kit is used for E.coli (DH5α) total RNA reverse-transcription into cdna and fruk gene PCR amplification. M: GM100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION Two-Step MMLV RT-PCR kit RP012-M 50 67 GeneMark

PCR Related Product PCR Related Product Features Characteristics: AMV reverse transcriptase possesses 5 to 3 DNA polymerase activity. Possess RNase H activity that breaks apart RNA:DNA hybrids. Effective amplification up to 6 kb DNA. Sensitivity: Eliminates primer-dimer formation and mis-priming. Components: Supplied as a 5X concentrated ready-to-use mix, that is, a 1X mixture of recombinant 1.5 U of Hot Start Taq DNA polymerase, reaction buffer, 2 mm MgCl 2, 250 μm dntp and enzyme stabilizer sufficient to allow efficient amplification of template in 50 μl PCR. Two-Step AMV RT-PCR Kit The GeneMark Two-Step AMV RT-PCR Kit has all the components required to perform first-strand cdna synthesis and second strand DNA amplification. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) can catalyze polymerization of DNA using DNA, RNA or DNA : RNA hybrids as templates. Besides possessing 5 to 3 DNA polymerase activity, the enzyme also possess some RNase H activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand complementary DNA (cdna) and primer extensions. In the cdna synthesis step, RNA was reverse transcribed by AMV RTase to produce its cdna. For the subsequent amplification of the cdna template, PCR Hot-Start Master Mix II is provided. It can eliminate amplification artifacts such as primer-dimer formation and mispriming during pre-amplification stage and thus may provide improved specificity. PCR Hot-Start Master Mix II can be used for effective amplification of DNA up to 6 kb in length. Application Second strand cdna synthesis, PCR and cloning. M 1 kb Figure 1. RT-PCR products of E.coli total RNA generated using Two-step AMV RT-PCR Kit. E.coli (DH5α) total RNA is used as template to generate cdna. E.coli fruk gene is further amplified using the same kit. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION Two-Step AMV RT-PCR kit RP012-A 20 GeneMark 68

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product PCR Cloning Kit & DNA Ligation Kit TOPO Cloning System TOPfast /TOPBlunt PCR Cloning Kit pgm-t PCR Cloning Kit SpeedLigA DNA Ligation Kit 69 GeneMark

PCR Related Product PCR Related Product Features Fast: Short reaction time (5 min) High Efficiency: >80% for < 3 kb inserts; Convenient: Need not use ligase for ligation. Components: TOPfast vector, reaction buffer, control plasmid and inserts, M13 primers, and competent cell. TOPfast PCR Cloning Kit TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase. Application For cloning PCR products with 3 A-overhang. M 0.3 1 2 3 (kb) 25 5 min N 0.3 kb 1 kb 2 kb 3 kb Total colonies 17 2884 3604 1584 1456 Blue (%) 94.1 1.5 1.4 2.8 42.3 White (%) 5.9 98.5 98.6 97.2 57.7 postive(%) 100 100 100 90 Blue/white screen and positive ratio. Figure 1. Colony PCR results from colonies carrying different sized inserts. PCR products of 0.3 kb (50 ng/µl) 1 kb (50 ng/µl) 2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers. For successful clones, the PCR result should be ~202 bp plus the insert size. M : Gen-KB DNA Ladder TOPfast Vector Map and Vector Cloning Site LacZα TOP Vector 3807 bp Insert Ordering Info DESCRIPTION Cat.No. PACKAGE TOPfast PCR Cloning Kit (Including competent cell) TOPfast PCR Cloning Vector (not including competent cell) GTOP01-20 20 GTOP01-40 40 GTOP01-V20 20 GTOP01-V40 40 GeneMark 70

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product Features Fast: Short reaction time (5 min) High Efficiency: >80% for < 3 kb inserts; Convenient: Need not use ligase for ligation. Components: TOPBlunt vector, reaction buffer, control plasmid and inserts, M13 primers, and competent cell. TOPBlunt PCR Cloning Kit TOPBlunt PCR Cloning Kit is suitable for cloning Pfu polymerase amplified PCR products with blunt ends at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient cloning of blunt-ended DNA fragments to the vector without using a regular ligase. Application For cloning PCR products with blunt ends. M 0.3 1 2 3 (kb) Figure 1. Colony PCR results from colonies carrying different sized inserts. PCR products of 0.3 kb (50 ng/µl), 1 kb (50 ng/µl), 2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPBlunt PCR Cloning Vectors, and colony PCR was performed using M13 primers. For successful clones, the PCR result should be ~202 bp plus the insert size. M : Gen100 DNA Ladder 25 5 min N 300 bp 1 kb 2 kb 3 kb Total colonies 36 2856 3664 1186 91 Blue (%) 83.3 16 2.2 0.3 12.1 White (%) 16.7 84 97.8 99.7 87.9 postive(%) 100 90 100 80 TOPBlunt Vector Map and Vector Cloning Site LacZα TOP Vector 3807 bp Blunt Insert Ordering Info DESCRIPTION Cat.No. REACTION TOPBlunt PCR Cloning Kit (Including competent cell) TOPBlunt PCR Cloning Vector (not including competent cell) GTOP02-20 20 GTOP02-V20 20 71 GeneMark

PCR Related Product PCR Related Product Features Economical: Unbeatable value for regular cloning. High Efficiency: >80% for < 3 kb inserts; ~40% for 4~6 kb inserts. Convenient: Accept amplified, blunt and sticky end DNA. Stable: T-vector backbone is stable and regularly sequenced. Rapid: Ligation can be achieved within an hour (two buffer systems). Components: T-vector, ligation system, control plasmid and primers, competent cell. pgm-t PCR Cloning Kit The pgm-t Vectors are linearized vectors with a single 3 -terminal thymidine at both ends. The T-overhangs at the insertion site greatly improved the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases. The pgm-t Vectors are high-copy-number vectors containing T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of the enzyme β-galactosidase. Insertional inactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates. Application For cloning PCR products generated by Taq DNA polymerases. 16 overnight / 22 1 hr Insert size N 300 bp 2 kb 3 kb 4 kb Total colonies 57 2076 / 1652 1528 / 632 120 / 89 65 / 24 White (%) 12.3 96.3 / 96.9 99 / 96.8 86.7 / 76.4 84.6 / 83.3 Blue (%) 87.7 3.7 / 3.1 1 / 3.2 13.3 / 23.6 15.4 / 16.7 Positive(%) 100 / 100 90 / 100 50 / 100 40 / 30 Blue/white screen and positive ratio. pgm-t Vector Map and Vector Cloning Site Ordering Info DESCRIPTION Cat.No. PACKAGE pgm-t Cloning Kit GVT202 20 pgm-t Cloning Kit (Competent cell not included) GVT202-0 20 pgm-t Cloning Vector (Ligase and buffer not included) GVT202-T1 GVT202-T2 20 60 GM DNA Ligation Kit GVT202-L 20 GeneMark 72

GeneMark GMbiolab Co., Ltd. PCR Related Product PCR Related Product SpeedLigA TM DNA Ligation Kit The SpeedLigA TM DNA Ligation Kit enables ligation of cohesive ends in 5 minutes at room temperature, and 5~15 minutes for blunt ends. PCR product TA cloning takes only an hour of incubation. Features Speed: DNA ligation completes within 5~20 minutes at room temperature (cohesive or blunt end). Easy: Can be used for DNA ligation, Linker addition, TA Cloning, library construction. Complete Packaging: All buffers and enzymes needed are included, need not to prepare separately. Application Suitable for cohesive or blunt ends, all common ligation, linker addition, TA cloning, library construction 700 bp 600 bp 300 bp Figure 1. pgem-7zf (+) DNA were ligated with 300 bp, 600 bp and 700 bp DNA fragments by SpeedLigA Kit. Lane 1: Before ligation, pgem-7zf (+) DNA/EcoRI and 300 bp, 600 bp and 700 bp/ecori DNA fragments. Lane 2: After 2 minutess at RT, then 70 15 minutes inactivation. Lane 3: After 5 minutes at RT, then 70 15 minutes inactivation. M: Gen100 DNA Ladder Ordering Info DESCRIPTION Cat.No. REACTION SpeedLigA TM DNA Ligation Kit SL01 30 SpeedLigA TM DNA Ligation Kit SL01-150 150 73 GeneMark

Modified Enzymes Cat # DNA Polymerases Nucleases DNA Polymerase I Large Fragment T4 DNA Polymerase (Exonuclease-free) RNase H (cloned) RNase A DNase I (RNase-free) Ribonuclease Inhibitor (DNase-Free) E325 E339 GR0610 RB0473 GM0649 RB0478-2KU Ligases T4 DNA Ligase E319 Kinase T4 Polynucleotide Kinase E311 Proteinase Proteinase K GM-PK0100 DNA Glycosylase Uracil DNA Glycosylase E335 DNA Dependent RNA Polymerases Reverse Transcriptase T3 RNA Polymerase T7 RNA Polymerase M-MuLV Reverse Transcriptase, RNase H- AMV Reverse Transcriptase SAMscript Reverse Transcriptase SuperSAMscript Reverse Transcriptase GMsensitive Reverse Transcriptase GT4905 GT7905 E317 B0999 GRT001 GRT002 GSR-1000 GeneMark 74

GeneMark GMbiolab Co., Ltd. Modified Enzymes Modified Enzymes DNA Polymerases Cat.No. E325 E326 SIZE 200 U 5 x 200 U DNA Polymerase I, Large Fragment (Klenow Fragment) DNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3 to 5 exonuclease activity, but has lost 5 to 3 exonuclease activity. Application Dideoxy sequencing, polishing ends, second strand cdna synthesis. Cat.No. E339 E340 SIZE 200 U 5 x 200 U T4 DNA Polymerase (Exonuclease-free) T4 DNA Polymerase catalyzes the synthesis of DNA in the 5 to 3 direction and requires the presence of template and primer. This enzyme has a 3 to 5 exonuclease activity. Application DNA synthesis, DNA Blunting. Nucleases Cat.No. GR0610 SIZE 250 U RNase H (cloned) Ribonuclease H catalyzes the specific degradation of RNA in DNA-RNA hybrids. None of the double or single-stranded DNA or RNA will be degraded. It is the enzyme in cdna synthesis and cloning procedures in which it removes the mrna during second-strand cdna synthesis. It can remove poly(a) tails on mrnas after hybridization with oligo(dt). It has also been used for the specific fragmentation of RNA after hybridization of the RNA with oligodeoxynucleotides. RNase H product is free of contaminating non-specific endonucleases, exonucleases and ribonucleases. Application cdna cloning by the Okayama-Berg method, detection of DNA-RNA hybrids and degradation of RNA, removal of the poly(a) tail from mrna in the presence of oligo(dt). 75 GeneMark

Modified Enzymes Modified Enzymes Cat.No. RB0473-100 RB0473-250 RB0473-500 RB0473-1g SIZE 100 mg 250 mg 500 mg 1 g Nucleases RNase A Pancreatic, high purity grade. This product is from a BSE free source and it does not come in contact with or have had BSE introduced into the product. Application Removal of RNA from preparation of plasmid DNA. Cat.No. GM0649 SIZE 2000 U /vial DNase I (RNase free) DNase I is lyophilized from Bovine Pancreas, and is essentially RNase free. It is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing oligodeoxyribonucleotides with 5 - phosphate and 3 -OH groups. Application RNase-free DNase I can be used for single- and double-stranded DNA digestion in RNA purification or any other RNA related applications. M 1 2 3 Figure 1. Gel electrophoresis analysis of total RNA treated with GeneMark RNase-free DNase I Cat#:GM0649). RNA products were treated with DNase I for 15 min at 37, and analyzed on 2 % agarose gel. Lane 1: 1 μg of total RNA purified using GeneMark total RNA purification kit Lane 2: 1 μg of total RNA treated with 2.4 U of DNase I (non-rnase free) Lane 3: 1 μg of total RNA treated with 1 U of DNase I (RNase-free) Cat.No. RB0478-2KU SIZE 2000 U Ribonuclease Inhibitor (DNase-free) RNase inhibitors are purified from human placenta or porcine liver. These inhibitors form a 1:1 complex with RNase A, and inhibit RNase activity noncompetitively. The inhibitor can easily be removed from the reaction system by phenol extraction, and does not inhibit the RNase H activity of Reverse Transcriptase. This product is free of exonuclease, endonuclease, RNase activities, and DTT should be present for activation of this protein. Application cdna synthesis, in vitro transcription, polysome isolation. GeneMark 76

GeneMark GMbiolab Co., Ltd. Modified Enzymes Modified Enzymes Ligases Cat.No. E319 E320 SIZE 4000 U 20000 U T4 DNA Ligase T4 DNA ligase is the most versatile and commonly used ligase for DNA cloning. This ATP-dependent enzyme covalently joins blunt or compatible cohesive ends, as well as nicks in double-stranded DNA. A 5 -phosphoryl group is required for ligation to a 3 -hydroxyl. Both 10x Reaction Buffer and 25 mm ATP solution are provided with the enzyme. For those interested in the shortest possible reaction time, GeneMark also offers SpeedLigA DNA Ligation Kits (Cat # SL01). This kit is specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning. Cohesive and blunt ends ligations can be performed in as little as 5 minutes. T4 DNA ligase product is free of detectable exo- and endonuclease and RNase activities. Application Ligation of blunt ends or compatible cohesive ends. 77 GeneMark

Modified Enzymes Modified Enzymes Kinase T4 Polynucleotide Kinase T4 polynuceotide kinase catalyzes the transfer and exchange of Pi from the ɣ-position of ATP to the 5 hydroxyl terminus of polynucleotides (double- and single-stranded DNA and RNA) and nucleoside 3 - monophosphates. The enzyme also catalyzes the removal of 3 -phosphoryl groups from 3 - phosphoril polynucleotides, deoxynucleoside 3 - monophosphates and deoxynucleoside 3 -diphosphates. This product is free of exonuclease, phosphatase, endonuclease and RNase activities. Cat.No. E311 E312 SIZE 500 U 5 x 500 U Application Addition of 5 -phosphates to oligonucleotides to allow subsequent ligation, removal of 3 -phosphoryl groups, end-labeling DNA or RNA for probes and DNA sequencing,. Proteinase Proteinase K Proteinase K is a non-specific serine protease isolated from Tritirachium album with a very high specific activity. The Proteinase K powder is highly soluble (>50 mg/ml), and commonly provided as a 20 mg/ml liquid stock solution. Purity(Native-PAGE): > 99.5% Activity Assay and Unit Definitions: One unit is defined as the amount of enzyme that will liberate 1 μmol of tyrosine per minute at 37, ph 7.5. This proteinase K has 34 U/mg of enzyme activity and none detectable with DNA or RNA contamination. Storage: Dry powder in -20 for a year and in 4 for six months. Application Proteinase K is used in isolating RNA, gdna and digesting unwanted proteins during DNA and RNA preparations. It is also used in glycoprotein modification and protein structure studies. Proteinase K is active with SDS, urea and EDTA. Cat.No. SIZE M 20 20 20 20 0 1 0.1 0.01 BSA (μg) ProK (μg) GM-PK0100 GM-PK0500 GM-PK1000 100 mg 500 mg 1 g 260 130 95 72 55 36 28 17 11 BSA 66.43 kda ProK 29.7 kda Figure 1. BSA digestion using different concentration of Proteinase K. 20 μg BSA is treated with Proteinase K (Prok) of different concentrations (lane 2~4; 1 μg, 0.1 μg. 0.01 μg, respectively.) BSA protein can be fully digested when treated with 0.01 μg of Proteinase K. M: Protein Marker GeneMark 78

GeneMark GMbiolab Co., Ltd. Modified Enzymes Modified Enzymes Cat.No. E335 SIZE 200 U DNA Glycosylase Uracil DNA Glycosylase E.coli Uracil-DNA Glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double stranded DNA, but not from oligomers (6 or fewer bases). The enzyme can be 95% heat killed by incubation at 95 for 10 minutes. Application As a probe for protein-dna interaction studies, site-directed mutagenesis. Memo 79 GeneMark

Modified Enzymes Modified Enzymes DNA Dependent RNA Polymerase Cat.No. GT4905 SIZE 5000 U T3 RNA Polymerase T3 RNA Polymerase is an enzyme coded by the DNA of phage T3. This enzyme requires DNA that contains the T3 promoter sequence as the template and NTPs as substrates to synthesize RNA complementary to the template downstream of the promoter. It has high specificity for T3 promoter, and is able to synthesize full length transcripts. Application Preparing labeled RNA probes for Northern or Southern hybridization, preparing precursors for RNA splicing reactions, producing capped mrna when a cap analog is used as primer, synthesis of dsrna for gene silencing or RNA interference. Cat.No. GT7905 SIZE 5000 U T7 RNA Polymerase T7 RNA Polymerase is an enzyme coded by the DNA of phage T7. This enzyme requires DNA that contains the T7 promoter sequence as the template and NTPs as substrates to synthesize RNA complementary to the template downstream of the promoter. It has high specificity for T7 promoter, and is able to synthesize full length transcripts. Application Preparing labeled RNA probes for Northern or Southern hybridization, preparing precursors for RNA splicing reactions, producing capped mrna when a cap analog is used as primer, synthesis of dsrna for gene silencing or RNA interference. GeneMark 80

GeneMark GMbiolab Co., Ltd. Modified Enzymes Modified Enzymes Reverse Transcriptase Cat.No. E317 E318 SIZE 5000 U 25000 U M-MuLV Reverse Transcriptase, RNase H- Molony Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is a RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cdna synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cdnas and therefore the enzyme is recommended for preparing long cdnas. (Source: Isolated from E.coli strain that carries the cloned Reverse transcriptase gene.) Application First strand cdna synthesis, especially long cdnas. Cat.No. B0999 SIZE 200 U AMV Reverse Transcriptase The AMV Reverse Transcriptase is a DNA polymerase which catalyzes the polymerization of DNA, using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5 to 3 DNA polymerase activity, the enzyme also possess some RNase H activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand cdna and primer extensions. To a lesser extent, it is used for RNA sequencing and the preparation of probes for hybridization. (Source: Avian Myeloblastosis Virus particles.) Application For the synthesis of first and second strand cdna, primer extensions, RNA sequencing, preparation of probes for hybridization. 81 GeneMark

Modified Enzymes Modified Enzymes Reverse Transcriptase SAMscript Reverse Transcriptase Cat.No. GRT001S GRT001L Package 10000 U 50000 U SAMscript Reverse Transcriptase uses both RNA and single stranded DNA as templat for synthesis of complementary DNA. This reverse transcriptase is capable of synthesizing DNA longer than 5 kb from mrna. SuperSAMscript Reverse Transcriptase Cat.No. Package GRT002S 2000 U GRT002M 10000 U GRT002L 10000 U x 4 SuperSAMscript Reverse Transcriptase is a genetically engineered version of MMLV reverse transcriptase. It is highly thermostable, thus can synthesize cdna at elevated temperature up to 60. This reverse transcriptase is capable of synthesizing DNA longer than 20 kb from mrna. Cat.No. GSR-1000S GSR-1000L Package 50 U 250 U GMsensitive Reverse Transcriptase GMsensitive Reverse Transcriptase is a mutant of MMLV reverse transcriptase with reduced RNase H activity and increased thermo stability. This reverse transcriptase possess high sensitivity and can synthesize cdna in reaction temperature at 50. GeneMark 82

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes A. Order List of Restriction Endonucleases......84 B. GM Buffer Activity Chart for Restriction Endonucleases...87 C. Double Digestion... 91 D. Compatible Cohesive Ends.....92 E. Isoschizomers......94 A AatII AccI Acc65I AciI AclI AcuI AfeI AflII AgeI AhdI AjnI AluI AlwI ApaI ApaLI ApoI AscI AseI AsiSI AspLEI AvaI AvaII AvrII B BamHI BanI BanII BbsI BbvI BclI BglI BglII BisI BlpI BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaJI BsiEI BsiHKAI BsiWI BslI BsmI BsmAI BsmFI Bsp1286I BspEI BspMI BsrI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI BstAPI BstBI BstEII BstF5I BstNI BstUI BstXI BstYI BstZ17I Bsu36I BtgI BtrI C Cac8I ClaI D DdeI DpnI DraI DraIII DrdI E EaeI EagI EarI EcolCRI EcoNI EcoRI EcoRV F FalI FatI FauI Fnu4HI FokI FspI G GlaI H HaeII HaeIII HgaI HhaI HincII HindIII HinfI HinP1I HpaI HpaII HphI HpyCH4III K KpnI Kzo9I M MboII MluI MnlI MscI MseI MslI MspI MspA1I MwoI N NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI P PciI PflMI PleI PmlI PpuMI PshAI PsiI Psp0MI PspGI PspXI PsrI PstI PvuI PvuII R RsaI RsrII S SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SnaBI SpeI SphI SspI StuI StyI SwaI T TaqI Tsp509I Tth111I X XbaI XhoI XmaI XmnI Z ZraI 83 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes Order List of Restriction Endonucleases GeneMark 84

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 85 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 86

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes GM Buffer Activity Chart for Restriction Endonucleases 87 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 88

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 89 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes * SAM: 0.1 mm S-adenosylmethionine (SAM) sould be added to the reaction mix. Optimal buffers contents (1X): 1. (B): 10 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 1 mm DTT. 2. (G): 10 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 50 mm Nacl; 1 mm DTT. 3. (O): 50 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 100 mm Nacl; 1 mm DTT. 4. (W): 10 mm Tris-HCL, ph 8.5 (at 25 ); 10 mm MgCl 2 ; 100 mm Nacl; 1 mm DTT. 5. (Y): 33 mm Tris-acetate, ph 7.9 (at 25 ); 10 mm Mg(CH 3 COO) 2; 60 mm KCH 3 COO; 1 mm DTT. 6. (K): 10 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 100 mm KCl; 1 mm DTT. 7. 2W: 20 mm Tris-HCL, ph 8.5 (at 25 ); 10 mm MgCl 2 ; 200 mm Nacl; 1 mm DTT. 8. 2K: 10 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 200 mm KCl; 1 mm DTT. 9. Bis I: 10 mm Tris-HCL, ph 9.0 (at 25 ); 10 mm MgCl 2 ; 150 mm KCl; 1 mm DTT. 10. Dpn I: 20 mm Tris-HCL, ph 9.0 (at 25 ); 10 mm MgCl 2 ; 150 mm NaCl; 1 mm DTT. 11. EcoR I: 100 mm Tris-HCL, ph 7.6 (at 25 ); 10 mm MgCl 2 ; 50 mm NaCl; 1 mm DTT. 12. Gla I: 10 mm Tris-HCL, ph 8.5 (at 25 ); 5 mm MgCl 2 ; 10 mm NaCl; 1 mm 2-mercaptoethanol. GeneMark 90

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes Double Digestion Cleavage of a DNA substrate with two restriction endonucleases simultaneously (double digestion) is a common, time-saving procedure. It is important to select the most suitable GM buffer for both restriction endonucleases. Each enzyme is supplied with its optimal GM buffer to ensure 100% activity. The GM buffer that results in the most activity for both enzymes should be used for double digestion. Double Digest Table Notes: + ----- BSA is required for double digestion * ----- In the table above, Sma I needs to work at 25, Eag I and 91 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes Compatible Cohesive Ends Restriction endonucleases that produce compatible cohesive ends often produce recleavable ligation products. The combinations listed were identified by computer analysis, and although we have tried to ensure their accuracy, they have not necessarily been confirmed experimentally. Where isoschizomers exist, only one member of each set is listed. Enzymes that have degenerate recognition sequences (i.e. recognize more than one sequence) are followed by a specific sequence in parentheses and are only listed if a non-degenerate equivalent does not exist. Be aware that these degenerate enzymes will cleave sequences in addition to the one listed. GeneMark 92

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 93 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes Isoschizomers Restriction endonucleases that recognize the same sequence are isoschizomers. The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from GeneMark. All recognition sequences are within 5 to 3 using the single letter code nomenclature with the point of cleavage indicated by ^. GeneMark 94

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 95 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 96

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 97 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 98

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 99 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 100

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 101 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 102

GeneMark GMbiolab Co., Ltd. Restriction Enzymes Restriction Enzymes Restriction Enzymes 103 GeneMark

Restriction Enzymes Restriction Enzymes Restriction Enzymes GeneMark 104

GeneMark GMbiolab Co., Ltd. Molecular Size Markers Molecular Size Markers Molecular Size Markers & Vector DNA DNA Markers, RNA Markers and Protein Markers GM50P 3000bp 1800bp 1100bp 750bp 500bp 450bp 400bp 350bp 300bp 250bp 200bp 150bp 100bp 50bp GM100 3.0 kb 2.0 kb 1.5 kb 1.0 kb 900 bp 800 bp 700 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp GM1000 14.0kb 10.0kb 8.0kb 6.0kb 5.0kb 4.0kb 3.0kb 2.5kb 2.0kb 1.5kb 1.0kb 0.5kb 0.2kb Ordering Info Product Name Cat. No. Scale Size Gen50 Plus DNA Ladder GM50P 50~3000 bp 500 µl/vial Gen100 DNA Ladder GM100 100~3000 bp 500 µl/vial Gen100 LC DNA Ladder GM100-LC 100~3000 bp 250 µl/vial GenKB DNA Ladder GM1000 0.2~14 kb 500 µl/vial GenKB LC DNA Ladder GM1000-LC 0.2~14 kb 500 µl/vial 1 kb DNA Ladder GLM11 250~10000 bp 50 µg 50 KB LC DNA Ladder, 50 ug M50K-50 250~48502 bp 250 µl/vial λ/hind III DNA Marker LH01 564 bp~23.1kb 500 µl/vial 16S, 23S RNA Marker DM0818 1776 3566 bp 2.5 mg 18S, 28S RNA Marker DM0817 2000 5300 bp 250 µg RNA Ladder DM0819 500~9,000 bp 50 µg Mid-Range Protein Marker GM47 14.4~97.4 kda 5 x 60 µl GenColor Prestained Protein Marker GMP0671 10~170 kda 500 µl/set 105 GeneMark

Molecular Size Markers Molecular Size Markers DNA Markers Gen50 Plus DNA Ladder Cat.No. GM50P SIZE 500 µl/vial 2.5%, 0.5X TAE, agarose gel Gen50-Plus DNA Ladder consists 14 liner double stranded DNA bands of 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 750, 1100, 1800 and 3000 base pairs. This Gen50-Plus DNA ladder is specially prepared for visualizing DNA fragments on the gel. The intensity of 300 bp band has been increased to yield reference indicator. 1μl 2μl 1 μl Cat.No. GM100 SIZE 500 µl/vial Gen100 DNA Ladder Gen100 DNA Ladder consists the following 13 DNA fragments: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 3000 base pairs. The intensity of the 500 bp, 1 kb, 1.5 kb, 2 kb, 3 kb bands have been increased to yield an internal reference indicator. 3.0 kb 30 ng 2.0 kb 16 ng 1.5 kb 16 ng 1.0 kb 8 ng 900 bp 5 ng 800 bp 5 ng 700 bp 5 ng 600 bp 5 ng 500 bp 20 ng 400 bp 5 ng 300 bp 5 ng 200 bp 5 ng 100 bp 8 ng 2%, 0.5X TAE agarose gel 1.5μl 3μl 1 μl 3.0 kb 20 ng 2.0 kb 10.6 ng 1.5 kb 10.6 ng Gen100 LC DNA Ladder (Low concentration of Gen100) Cat.No. SIZE 1.0 kb 5.4 ng 900 bp 3.4 ng 800 bp 3.4 ng 700 bp 3.4 ng 600 bp 3.4 ng 500 bp 13.4 ng 400 bp 3.4 ng 300 bp 3.4 ng 200 bp 3.4 ng 100 bp 5.4 ng Gen100 LC DNA Ladder consists the following 13 DNA fragments: 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000 and 3000 base pairs. The intensity of the 500 bp, 1 kb, 1.5 kb, 2 kb, 3 kb bands have been increased to yield an internal reference indicator. GM100-LC 250 µl/vial 2%, 0.5X TAE agarose gel GeneMark 106

GeneMark GMbiolab Co., Ltd. Molecular Size Markers Molecular Size Markers DNA Markers 1μl 1.5μl 1 μl 14.0 kb 6 ng 10.0 kb 10 ng 8.0 kb 10 ng 6.0 kb 12 ng 5.0 kb 12 ng 4.0 kb 10 ng 3.0 kb 60 ng 2.5 kb 10 ng 2.0 kb 12 ng 1.5 kb 10 ng 1.0 kb 10 ng GenKB DNA Ladder GenKB DNA Ladder consists the following 13 DNA fragments: 0.2 kb, 0.5kb, 1 kb, 1.5 kb, 2 kb, 2.5 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb and 14 kb. The intensity of the 0.5 and 3 kb bands have been increased to yield an internal reference indicator. Cat.No. SIZE 0.5 kb 30 ng GM1000 500 µl/vial 0.2 kb 10 ng 0.8%, 0.5X TAE agarose gel 2μl 3μl kb 1 μl Cat.No. SIZE GenKB LC DNA Ladder (Low concentration of GenKB) GenKB LC DNA Ladder consists the following 13 DNA fragments: 0.2 kb, 0.5kb, 1 kb, 1.5 kb, 2 kb, 2.5 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb, 10 kb and 14 kb. The intensity of the 0.5 and 3 kb bands have been increased to yield an internal reference indicator. 14.0 kb 3 ng 10.0 kb 5 ng 8.0 kb 5 ng 6.0 kb 6 ng 5.0 kb 6 ng 4.0 kb 5 ng 3.0 kb 30 ng 2.5 kb 5 ng 2.0 kb 6 ng 1.5 kb 5 ng 1.0 kb 5 ng 0.5 kb 15 ng 0.2 kb 5 ng GM1000-LC 500 µl/vial 0.8%, 0.5X TAE agarose gel bp 1 KB DNA Ladder Cat.No. SIZE The 1 Kb DNA Ladder consists the following 13 DNA fragments: 250 bp, 500 bp, 750 bp, 1 kb, 1.5 kb, 2 kb, 2.5 kb, 3 kb, 4 kb, 5 kb, 6 kb, 8 kb and 10 kb. The intensity of the 1 and 3 kb bands have been increased to yield an internal reference indicator. GLM11 50 µg 107 GeneMark 1%, TAE agarose gel

Molecular Size Markers Molecular Size Markers DNA Markers Cat.No. SIZE M50K-50 50 µg 50 KB LC DNA Ladder The 50 KB DNA Ladder has a number of proprietary plasmids and phages DNA which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose pulsedfield gel electrophoresis(pfge). bp 0.8% 0.5X TAE agarose gel 23.1 kb Cat.No. SIZE 9.4 kb 6.6 kb 4.4 kb 2.3 kb 2.0 kb 564 bp λ/hind III DNA Marker Lambda DNA Ladder is completely digested by Hind III, phenol extracted, ethanol precipitated and dissolved in 10 mm Tris-HCI (ph7.6) and 1 mm EDTA. Hind III digest of λdna yields 7 DNA fragments: 564, 2027, 2322, 4361*, 6557, 9416 and 23130*. LH01 500 µl/vial 0.7%, 0.5X TAE agarose gel * Before loading, heat the marker at 65 C for 5 minutes then cooling on ice, or 4.4 kb may anneal to 23.1 kb. GeneMark 108

GeneMark GMbiolab Co., Ltd. Molecular Size Markers Molecular Size Markers Protein Markers 1.5 μl 3 μl Middle Range Protein Marker 97.4 kd 66.2 kd Cat.No. SIZE This marker is a ready to use marker, and is consisted of the following 6 proteins: Lysozyme 14.4 kda, Soybean trypsin inhibitor 20.5 kda, Carbonic Anhydrase 29 kda, Chicken Egg Albumin 45 kda, Bovine Serum Albimin 66.2 kda and Phosphorylase B (rabbit muscle) 97.4 kda. 45 kd 29 kd GM47 5 x 60 µl (300 µl) Usage: 2 or 3 μl as a load is used for clear banding pattern. Storage: Store at -20 after reconstitution 20.5 kd 14.4 kd GenColor Prestained Protein Marker Cat.No. GMP0671 SIZE 500 µl/set The GenColor Prestained Protein Marker is designed for visualization of protein molecular weight during SDS PAGE electrophoresis, and verification of Western transfer efficiency. The GenColor Prestained Protein Marker consists 10 prestained proteins covering the range from 10~180 kda. Proteins are covalently coupled to a blue dye, except the fourth protein band from the top, which is coupled to a green dye, and eighth protein band from the top, which is coupled to a red dye. GeneMark 109

Molecular Size Markers Molecular Size Markers Vector DNAs Cat.No. MDD22 MDD23 SIZE 0.1 mg 0.5 mg Lambda DNA Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction enzymes activities. Phage is isolated from heat inducible lysogen E.coli W3350 (cl857 Sam7) strain. DNA was isolated from purified phage by phenol extraction and dialyzed against 10 mm Tris-HCl (ph7.6) and 1 mm EDTA. It contains 48502 bases. Concentration: 500 μg/ml Store at -20. Cat.No. MSD12 MSD13 SIZE 0.1 mg 0.5 mg pt7 DNA Bacteriophage T7 Purified from bacteriophage T7. Homogenous using agarose gel electrophoresis. Concentration: 0.5 mg/ml Cat.No. SD1161 SD1163 SIZE 0.05 mg 0.25 mg puc18 DNA puc18 DNA was isolated from E.coli strain DH5α. Purified by ultra-centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded and 2686 base pairs in length. Concentration: 0.3~0.5 mg/ml Cat.No. MSD16 MSD17 SIZE 0.05 mg 0.25 mg puc19 DNA puc19 DNA was isolated from E.coli strain DH5α. Purified by ultra-centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded and 2686 base pairs in length. Concentration: 0.2 mg/ml GeneMark 110

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals & Solutions Biochemicals Tris, Glycine, Sodium chloride, MOPS, DTT, MTT, IPTG, NBT, BCIP, X-Gal, X-Gluc Ready to use buffers RNA related solutions Nucleic acid / Protein stain solutions dntp solutions Antibiotic solutions Other solutions 50X TAE Buffer 20X SSC Buffer 10X TGS Buffer 10X PBS Buffer 20X SSPE Buffer 10X TBST Buffer 5X TBE Buffer 10X TG Buffer 10X TE Buffer 10X TBS Buffer 10X TEN Buffer RNase DNase Away Solution RNase A stock (DNase-free) RNA-SafeGuard Reagent (20X) TriSolution Plus Reagent RNAfter TM DEPC treated Water Ethidium Bromide Solution SafeView DNA Stain InstantBlue Gel Staining Reagent 2.5 mm, 10 mm, 100 mm (each, ATGC) Ampicillin, Chloramphenicol, G418, Gentamicin, Hygromycin B, Kanamycin, Neomycin, Penicillin-G, Rifampicin, Spectinomycin Denhardt's Solution (100X) Glycogen Solution Salmon Sperm DNA Solution GeneMark 111

Biochemicals&Solutions Biochemicals & Solutions Biochemicals ACES [N-(Carbamoylmethyl)-2-aminoethane sulfonic acid] High Purity grade Cat. No. GMSH001-0100 GMSH001-0500 Size 100 g 500 g C 4 H 10 N 2 O 4 S MW 182.20 Cas # 7365-82-4 Assay: 99.5% Water: 0.19% Heavy Metals (as Pb): 1 ppm Infrared: Confirm to reference UV absorbance (260 nm; 280 nm): 0.005; 0.002 Abs unit ph (1% water solution 25 ): 3.9 Solubility: Clear, colorless solution White crystalline powder. Ammonium acetate AR grade GJDH06042-010 500 g CH 3 COONH 4 MW 77.08 Cas # 631-61-8 Assay: 98.3% ph value: 7.0 Clarity of Solution, NO: 3 Sulfate: 0.0015% Chloride: 0.0003% Nitrate: 0.0005% Phosphate: 0.0003% Magnesium: 0.0001% Calcium: 0.0003% Iron: 0.0003% Heavy metals (as Pb): 0.0003% Insoluble matter in water: 0.005% Substances reducing potassium permanganate(as HCOOH): 0.0032% Ammonium bicarbonate (Ammonium hydrogen carbonate) AR grade GJDH06074-010 500 g NH 4 HCO 3 MW 79.06 Cas # 1066-33-7 Assay (as NH 3 ): 21.2% Chloride: 3 ppm Sulfate: 0.001% Iron: 1 ppm Heavy metals (as Pb): 4 ppm Insoluble matter in water: 0.002% Residue after ignition (as sulfate): 0.0018% Ammonium carbonate AR grade GJDH06001-010 500 g (NH 4 ) 2 CO 3 MW - Cas # 506-87-6 Assay: 42.8% Iron: 0.5 ppm Heavy metals (as Pb): 3 ppm Chloride: 3 ppm Sulfur compounds (as SO 4 ): 0.0015% Residue after ignition(as sulfate): 0.0012% Insoluble matter in water: 0.002% Ammonium chloride AR grade GJDH06007-040 500 g NH 4 Cl MW 53.49 Cas # 12125-02-9 Assay: 99.9% Magnesium: 0.00004% Phosphate: 0.0003% Calcium: 0.0008% Sulfate: 0.003% ph value (50g/L, 25 ): 4.8 Potassium: 0.0007% Iron: 0.0003% Insoluble matter in water: 0.005% Residue after ignition(as sulfate): 0.009% Ammonium persulfate (APS) AR grade GJDH06075-015 25 g (NH 4 ) 2 S 2 O 8 MW 228.20 Cas # 7727-54-0 Assay: 98.0% Manganese: 0.3 ppm Iron: 3 ppm Chloride and chlorate (as Cl): 10 ppm Heavy metals (as Pb): 3 ppm Residue after ignition(as sulfate): 0.02% Insoluble matter in water: 0.005% White powder. Very soluble in water. Ammonium sulfate AR grade GJDH06005-021 GJDH06005-025 1 kg 5 kg (NH 4 ) 2 SO 4 MW 132.14 Cas # 7783-20-2 Assay: 99.5% Phosphate: 0.0003% Arsenic: 0.00001% Iron: 0.0001% Nitrate: 0.001% ph value(50g/l, 25 ): 5.3 Heavy metals (as Pb): 0.0003% Calcium: 0.003% Chloride: 0.0003% Residue after ignition(as sulfate): 0.0003% Insoluble matter in water: 0.002% GeneMark 112

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Cat. No. GMSJ003-0500 GMSJ003-1000 GMSJ003-5000 Size 500 mg 1 g 5 g BCIP, toluidine salt (5-Bromo-4-chloro-3-indolyl phosphate toluidine salt) Ultra Pure grade C 8 H 6 BrClNO 4 PC 7 H 9 N MW 433.62 Cas # 6578-06-9 Assay: 99% Heavy metals: 2 ppm Water: 0.3% White powder. Soluble in DMF (20 mg/ml), insoluble in water. Benzoic acid AR grade C 6 H 5 COOH MW 122.12 Cas # 65-85-0 Assay: 99.5% Iron: 3 ppm Sulfur compounds: 0.003% Chlorine compounds: 0.009% MP: 122.9~123.0 Heavy metals (as Pb): 5 ppm Residue after ignition: 0.006% GJDH14115-020 250 g Substances reducing potassium permanganate: Passes test Substances darkened by sulfuric acid: Passes test BICINE [N,N-Bis(2-hrdroxyethyl)glycine] High Purity grade GMSH004-0500 GMSH004-1000 500 g 1 kg C 6 H 13 NO 4 MW 163.0953 Cas # 150-25-4 Assay: 100.2% Loss on drying: 0.05% Heavy metals: 3 ppm ph (1M solution in H2O,20 ): 4.9 UV absorbance(260 nm; 280 nm): 0.037; 0.025 Abs unit White crystal. Boric acid AR grade GJDH14001-041 GJDH14001-045 1 kg 5 x1 kg H 3 BO 3 MW 61.83 Cas # 10043-35-3 Assay: 99.9% Chloride: 0.0001% Sulfate: 0.001% Phosphate: 0.0001% Arsenic: 0.00005% Calcium: 0.0004% Iron: 0.0001% Lead: 0.0001% Insoluble matter in water: 0.005% Substances not volatilized by methanol: 0.03% Bromocresol green, free acid (3,3,5,5 -Tetrabromo-m-creslsulfonphthalein) IND grade C 21 H 14 Br 4 O 5 S MW 698.02 Cas # 76-60-8 Maximum absorption wavelength: λ 1 (ph3.8): 445 nm λ 2 (ph5.4): 617 nm Mass absorbance: α 1 (λ 1,pH3.8,dried basis): 24 L/cm g α 2 (λ 2,pH5.4,dried basis): 53 L/cm g GJDH28449-040 10 g Residue after ignition(as sulfate): 0.25% Solubility test in ethanol: Passes test Loss on drying: 2.6% Visual transition interval(3.8(olivine)~5.4(blue)): Passes test Bromocresol purple, free acid (5,5 -Dibromo-o-cresolsulfonphthalein) IND grade C 21 H 16 Br 2 O 5 S MW 540.23 Cas # 115-40-2 Loss on drying: ND Residue after ignition(as sulfate): 0.08% Solubility test in dilute base: Passes test GJDH28827-030 10 g Appearance: Passes test Visual transition interval(5.2(yellow)~6.8(purple)): Passes test Bromophenol blue (3,3,5,5 -Tetrabromophenolsulfonphthalein) IND grade GJDH28625-030 10 g C 19 H 10 Br 4 O 5 S MW 669.97 Cas # 115-39-9 Visual transition interval: Passes test Mass absorbance: 115.5 L/cm g Solubility test in ethanol: Passes test Residue after ignition(as sulfate): 0.12% GeneMark 113

Biochemicals&Solutions Biochemicals & Solutions Biochemicals Cat. No. GMSQ003-0025 GMSQ003-0100 Size 25 g 100 g Bovine Serum Albumin (BSA), ph6.5~7.2 Biotech grade MW ~66 kda Cas # 9048-46-8 Assay: >98% ASH: 1.5% Fatty acid: 0.24 mg/g ph: 6.5~7.5 IgG: Negative Solubility: 0.25 Heavy metals: 10 ppm Loss on drying (105, 3 hours): 5% Calcium carbonate AR grade CaCO 3 MW 100.09 Cas # 471-34-1 Assay: 99.0% Chloride: 0.002% Sulfate: 0.008% Sodium: 0.0008% Potassium: 0.0002% Magnesium: 0.0004% Strontium: 0.003% Barium: 0.01% Iron: 5 ppm GJDH03302-020 500 g Alkalinity: 0.1 mmol/100 g Insoluble matter in hydrochloric acid: 0.01% Heavy metals (as Pb): 5 ppm Appearance of solution: Passes test Calcium chloride anhydrous AR grade GJDH03377-010 500 g CaCl 2 MW 110.98 Cas # 10043-52-4 Assay: 98.1% Sulfate: 0.02% Arsenic: 0.0001% Phosphate: 0.002% Iron: 0.001% Zinc: 0.003% Barium: 0.008% Nitrate: Passes test Free alkali: 0.015 mmol/g Insoluble matter: Passes test Heavy metals (as Pb): 0.0008% Magnesium and alkali metals(as sulfate): 0.15% Calcium chloride dihydrate AR grade GJDH03378-010 1 kg CaCl 2 2H 2 O MW 147.02 Cas # 10035-04-8 Assay: 98.9% ph value (450g/L,25 ): 8.0 Sulfate: 0.007% Barium: 0.003% Iron: 0.0005% Magnesium and alkali metals (as sulfate): 0.12% Calcium sulfate AR grade GJDH03306-010 500 g CaSO 4 2H 2 O MW 172.17 Cas # 7778-18-9 Assay: 99.9% Chloride: 0.0015% Nitrate: 0.0015% Ammonium: 0.003% Iron: 3 ppm Carbonate: 0.05% Heavy metals (as Pb): 7 ppm Insoluble matter in hydrochloric acid: 0.025% Alkali metals and magnesium: 0.19% Casein BR grade GJDH24427-010 250 g - MW - Cas # 9000-71-9 Water: 11.9% Fat: 0.4% Ash: 1.8% ph value(50g/l,25 ): 3.7 CHAPS [3-[3-Chloamidopropyl)dimethyl-ammonio]-1-propanesulfonate] Ultra Pure grade GMSH009-0005 GMSH009-0025 GMSH009-0100 5 g 25 g 100 g C 32 H 58 N 2 O 7 S MW 614.88 Cas # 75621-03-3 Assay: 100.2% Moisture: 0.8% Nitrogen (Kjeldahl): 4.56% Conductivity (0.1 M in H 2 O): 10 μs/cm TLC (Silica gel :methanol/ NH 4 OH=95/5): One Spot White powder. Soluble in water Solubility (10% in H 2 O, 20 ): Complete colorless GeneMark 114

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Congo red IND grade Cat. No. GJDH28041-010 Size 25 g C 32 H 22 N 6 Na 2 O 6 S 2 MW 696.68 Cas # 573-58-0 Loss on drying: 1.5% Visual transition interval: Passes test Insoluble matter in water: 0.24% Residue after ignition(as sulfate): 23.1% Crystal violet (Hexamethylpararosaniline chloride; Methyl violet; Gentian Violet; Basic violet 3) AR grade GJDH28803-010 25 g C 25 H 30 N 3 Cl MW 407.98 Cas # 548-62-9 Loss on drying: 6.0% Insoluble matter in ethanol: 0.1% Residue after ignition(as sulfate): 1.4% Absorbance test: Passes test Sensitivity test: Passes test D-Glucose, Anhydrous AR grade GJDH18392-010 GJDH18392-010 1 kg 5x1 kg C 6 H 12 O 6 MW 180.16 Cas # 50-99-7 Loss on drying: 0.5% Chloride: 0.001% Sulfate: 0.002% Calcium: 0.0015% Iron: 0.001% Acidity (as H+): 0.0015 mmol/g Heavy metals (as Pb): 3 ppm Sugar(sucrose): Passes test Dextrine and starch: Passes test Specific optical rotation : +52.9 Residue after ignition(as sulfate): 0.05% Appearance of solution: Passes test Diethanolamine AR grade GJDH20208-018 500 ml C 4 H 11 NO 2 MW 105.14 Cas # 111-42-2 Assay: 99.6% Crystallizing point: 27.7 Density(20 ): 1.096g/ml Residue after ignition : 0.02% Dimethyl sulfoxide (DMSO) AR grade C 2 H 6 OS MW 78.13 Cas # 67-68-5 Assay: 99.7% Density(25 ):1.102 g/mol Refractive index: 1.477 Crystallizing point:18.2 GJDH23041-010 500 ml Solubility test in water: Passes test Residue after ignition (as sulfate) : 0.1% Free acid (as H 2 SO 4 ) : 0.0001 mmol/g Disodium hydrogen phosphate (Sodium phosphate, monobasic, dihydrate) ) AR grade GJDH01503-020 500 g NaH 2 PO 4 2H 2 O MW 156.01 Cas # 13472-35-0 Assay: 99.4% Ammonium: 0.002% Potassium: 0.0076% Iron: 10 ppm Arsenic: 1 ppm Chloride: 0.005% Sulfate: 0.005% Nitrate: 10 ppm ph (50g/L,25 ): 4.5 Substances precipitated by ammonia: 0.01% Heavy metals: 10 ppm Appearance of solution: Passes test Insoluble matter in water: 0.01% D-Maltose monohydrate BR grade GJDH18331-010 GeneMark 115 250 g C 12 H 22 O 11. H 2 O MW 360.32 Cas # 6363-53-7 Sulfate: 0.01% Barium: Passes test Chloride: 0.007% ph value(50g/l, 20 ) : 6.0 Specific rotation: +131.94 Heavy metals (as Pb): 0.0008% Residue after ignition (as sulfate): 0.005% Solubility test in water: Passes test

Biochemicals&Solutions Biochemicals & Solutions Biochemicals DTT (DL-Dithiothreitol) Ultra Pure grade Cat. No. GMSJ009-0005 GMSJ009-0005 Size 5 g 25 g C 4 H 10 O 2 S 2 MW 154.2 Cas # 3483-12-3 Assay: 99.40% Clarity (5%(w/v)solution in water) : Clear and Colorless Oxidized DTT level : 0.2% 5% solution at ph4.5 : Clear and Colorless White to off-white free flowing powder. Hygroscopic. Eosin Y alcohol solution (Eosin Yellowish; Acid Red 87; Bronze Bromo ES; Solvent red 43; 2',4',5'7 -Tetrabromofluorescein) BS grade GJDH28009-030 25 g C 20 H 8 Br 4 O 5 MW 647.89 Cas # 15086-94-9 Biological stain test: Passes test Red to brown powder. Soluble in alkaline solutions and ethanol. Eosin Y water solution (2',4',5'7'-Tetrabromofluorescein disodium salt) IND grade GJDH28010-020 25 g C 20 H 6 Br 4 O 5 Na 2 MW 691.86 Cas # 17372-87-1 Loss on drying: 9.0% Halide(as Br):1.0% Mass absorbance:120 L/cm g Sensitivity test: Passes test Solubility test in water: Passes test Maximum absorption wavelength: 515 nm Ethanolamine (Monoethanolamine, 2-Aminoethanol AR grade GJDH20155-018 500 ml C 2 H 7 NO MW 61.08 Cas # 141-43-5 Assay: 99.9% Density(20 ):1.016 g/ml Crystallizing point: 10.0 Mixing test with water: Passes test Residue after evaporation: 0.003% Ethylenediaminetetraacetate tetrasodium salt, EDTA-Na4 AR grade GJDH01690-010 500 g C 10 H 12 N 2 Na 4 O 8 MW 416.20 Cas # 64-02-8 Assay: 99.0% Iron: 0.001% Complexation test: Passes test Gelatin AR grade GJDH23256-010 500 g - MW - Cas # 9000-70-8 Loss on drying: 11.8% Residue after ignition (as sulfate): 0.4% Arsenic: 1 ppm Heavy metals (as Pb): 0.003% Substances reducing fehling reagent (as maltose): Passes test Glycerin Glycerol AR grade C 3 H 8 O 3 MW 92.09 Cas # 56-81-5 Assay: 99.9% Color (APHA): 10 Residue after ignition (as sulfate): 9 ppm Iron: 0.5 ppm Arsenic: 0.3 ppm Sulfate: 4 ppm Chloride: 0.5 ppm Alkalinity (as OH-): ND Acidity (as H+): 0.0003 mmol/g Ammonium: 3 ppm Heavy metals (as Pb): 0.5 ppm Fatty acid ester(as glycerol tributyrate): 0.03% Sucrose and glucose: Passes test Substances reducing silver: Passes test GJDH17062-020 500 ml Readily carbonizable substances: Passes test GeneMark 116

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Glycine Biotech grade Cat. No. GMPT008-1000 GMPT008-5000 Size 1 kg 5x1 kg C 2 H 5 NO 2 MW 75.07 Cas # 56-40-6 Assay: 99.5% Loss on drying: 0.08% Chloride: 0.006% Sulfate: 0.0065% Arsenic(AS)PPM: 2% Residue on ignition: 0.04% Heavy Metals: 0.002% Hydrolyzable substances: Approved White crystalline free flowing powder. Guanidine hydrochloride Biotech grade GMPZ007-0500 GMPZ007-1000 500 g 1 kg CH 5 N 3. HCl MW 95.53 Cas # 50-01-1 Assay: 99.85% Loss on drying: 0.18% Melting point: 183.5 ph: 6.51 White crystalline powder. Guanidine Thiocyanate (Guanidine isothiocyanate; Guanidine rhodanide) Biotech grade GMPZ009-0500 500 g CH 5 N 3 HSCN MW 118.16 Cas # 593-84-0 Assay: 99.61% Loss on drying: 0.28% MP: 117 Water-Insoluble matter: 0.02% GMPZ009-1000 1 kg Aspect of 35% aqueous solution: Passes test White crystalline powder. HEPES, free acid (N-(2-Hydroxyethyl) piperazine-n -(2-ethanesulfonic acid) High Purity grade C 8 H 18 N 2 O 4 S MW 238.31 Cas # 7365-45-9 Assay: 99.8% PKa at 20 : 7.5 Residue on Ignition: 0.03% Loss on drying (105, 3 hours): 0.1% Sulfate: 0.008% Heavy metals(pb): 4 ppm UV absorbance (260 nm): 0.302 UV absorbance (280 nm): 0.205 UV absorbance (440 nm): 0.055 GMSH0011-0500 500 g Color (1 M solution): Passes test Free flowing white powder. Imidazole (Glyoxaline) Reagent grade GMPT001-0500 500 g C 3 H 4 N 2 MW 68.08 Cas # 288-32-4 Assay(HPLC): 99.6% Loss on drying: 0.32% MP: 90 White crystalline powder. IPTG (Isopropyl-β-D-1-thiogalatopyranoside) Dioxane Free Ultra Pure grade GMSJ0012-0005 GMSJ0012-0010 GMSJ0012-0100 5g 10 g 100 g C 9 H 18 O 5 S MW 238.3 Cas # 367-93-1 Assay(HPLC): 99.5% Purity(HPLC): 99.68% ph 5% in water: 6.54 Melting range: 110 ~113 Specific Optical Rotation: -31.74 Identification by IR: Approved UV absorbance(300 nm; 400 nm): 0.13; 0.04 Moisture content by KF: 0.23% w/w 1.4-Dioxane: Not detected White crystalline powder. Soluble in water and methanol. GeneMark 117

Biochemicals&Solutions Biochemicals & Solutions Biochemicals Lactic acid (2-Hydroxypropionic Acid) AR grade C 3 H 6 O 3 MW 90.08 Cas # 50-21-5 Assay: 87.9% Sulfate: 0.004% Chloride: 0.001% Aldehyde: Passes test Iron: 0.0007% Cat. No. GJDH14037-010 Size 500 ml Heavy metals (as Pb): 0.0003% Mixing test with water: Passes test Substances darkened by sulfuric acid: Passes test Mixing test with ethers: Passes test Residue after ignition: 0.018% Magnesium acetate, tetrahydrate AR grade GJDH03138-010 500 g C 4 H 6 MgO 4 4H 2 O MW 214.45 Cas # 16674-78-5 Assay: 99.9% Chloride: 3 ppm Sulfate: 0.002% Phosphate: 4 ppm Iron: 1.5 ppm Barium: 0.002% Calcium: 0.003% Manganese: 6 ppm Heavy metals: 3 ppm Insoluble matter in water: 0.005% Appearance of solution: Passes test Magnesium chloride, hexahydrate AR grade MgCl 2 6H 2 O MW 203.30 Cas # 7791-18-6 Assay: 98.7% ph value(50g/l,25 ): 5.6 Sulfate: 0.002% Phosphate: 4 ppm Iron: 1 ppm Calcium: 0.0008% Total nitrogen: 0.003% Barium and strontium: 0.002% GJDH03109-010 500 g Heavy metals (as Pb): 2 ppm Insoluble matter in water: 0.005% Appearance of solution: Passes test Magnesium sulfate, heptahydrate AR grade MgSO 4 7H 2 O MW 246.47 Cas # 10034-99-8 Assay: 99.6% Arsenic: 1 ppm Sodium: 0.00013% Chloride: 3 ppm Iron: 4 ppm GJDH03107-010 500 g ph value (50g/L, 25 ) : 5.7 Calcium: 0.0013% Manganese: 8 ppm Heavy metals (as Pb): 4 ppm Insoluble matter in water: 0.005% Appearance of solution: Passes test Manganese(II) chloride, tetrahydrate AR grade GJDH10554-010 500 g MnCl 2 4H 2 O MW 197.91 Cas # 13446-34-9 Assay: 99.7% Sulfate: 0.003% Zinc: 0.0006% Barium: 0.004% Iron: 1.3 ppm Heavy metals (as Pb): 4 ppm Alkali metals and alkali earth metals: 0.18% Insoluble matter in water: 0.005% Mannitol Oxidizing and reducing substances: Passes test AR grade GJDH17067-010 100 g C 6 H 14 O 6 MW 182.17 Cas # 69-65-8 Chloride: 0.001% Acidity (as H+): 0.0004mmol/g Specific optical rotation: +23 Residue after ignition (as sulfate): 0.04% Insoluble matter in water: 0.01% Melting range: 167.8~168.1 Heavy metals (as Cu): 3 ppm Loss on drying: 0.05% Substances not precipitated by ammonium sulfide: Passes test GeneMark 118

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals 2-Morpholinoethanesulfonic Acid (MES) High Purity grade Cat. No. GMSH0013-0100 GMSH0013-0500 GMSH0016-0500 Size 100 g 500 g 500 g C 6 H 13 NO 4 S MW 195.2 Cas # 4432-31-9 Assay: 99.5% PKa at 20 : 6.2 Heavy metals (Pb): 3 ppm Sulfate: 15 ppm Fe: 0.7 ppm Chloride: 75 ppm Free flowing white powder. Color of 1 M solution: Clear MOPS (3-(N-Morpholino) propane-sulfonic acid) High Purity grade C 7 H 15 NO 4 S MW 209.3 Cas # 1132-61-2 Assay 100.4% ph(1% solution at 25 ): 4.2 Pb: 2 ppm Fe: 0.6 ppm PKa at 20 : 7.16 Loss on drying(105, 3 hours): 0.02% UV absorbance (260nm): 0.005 Free flowing white powder. Solution (10%) in water: Passes Test MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2h-tetrazoliumbromide;) High Purity grade GMSJ0014-0001 GMSJ0014-0005 GMSJ0014-0025 GMSJ0015-0500 GMSJ0015-1000 GMSJ0015-1000 1 g 5 g 25 g 500mg 1 g 5 g C 18 H 16 BrN 5 S MW 414.33 Cas # 2348-71-2 Assay: 98.8% Heavy metals: 2 ppm Water Content: 0.1% White powder. Suitable for cell and insect cell culture. NBT (Nitro Blue Tetrazolium Chloride) High Purity grade C 40 H 30 N 10 O 6 Cl 2 MW 817.6 Cas # 298-83-9 Assay: 99.2% Heavy metals: 2 ppm Water content: 0.26% White powder. Oxalic acid, dihydrate (Ethanedioic acid) AR grade GJDH14036-036 500 g C 2 H 2 O 4 2H 2 O MW 126.07 Cas #6153-56-6 Assay: 99.5% Chloride: 0.001% Sulfate: 0.002% Total nitrogen: 0.001% Calcium: 0.002% Iron: 3 ppm Heavy metals (as Pb): 4 ppm Insoluble matter in water: 0.005% Readily carbonizable substances: Passes test Residue after ignition: 0.005% PIPES (Piperazine-1, 4-bisethanesulfonic acid) High Purity grade C 8 H 18 N 2 O 6 S 2 MW 302.4 Cas # 5625-37-6 Assay: 99.5% PKa at 20 : 6.77 Pb: 3 ppm Insoluble matter: 0.03% GMSH0020-0500 500 g Residue After Ignition: 0.09% Free flowing white powder. GeneMark 119

Biochemicals&Solutions Biochemicals & Solutions Biochemicals pnpp, 4-Nitrophenylphosphate, disodium salt, hexahydrate Reagent grade Cat. No. Size C 6 H 4 NNaO 6 P 6H 2 O MW 371.14 Cas # 4264-83-9 Purity (Tit): 100.2% Purity (enzyme; ALP): 95.4% Water Content (K.Fischer): 29.7% Solubility in water (1.4%)(A): Passes Test Serum activity: 100.5% GMSJ0017-0005 5 g Free p-nitrophenol (A): 0.205 (415 nm) IR spectrum: Authentic GMSJ0017-0025 25 g Slightly yellow white powder. Keep tightly closed. Store in a cool (0~5 )and dry place. Polyethylene glycol 4000 (PEG 4000) IMP grade GJDH17138-010 500 g HOCH 2 CH 2 (OCH 2 CH 2 ) n OH MW about 4000 Cas # 25322-68-3 Store: 18~25 C Molecular mass average: 4354.8 ph value (50g/L,25 ): 5.4 Polyethylene glycol 6000 (PEG 6000) IMP grade GJDH17139-010 500 g HOCH 2 CH 2 (OCH 2 CH 2 ) n OH MW about 6000 Cas # 25322-68-3 Store: 18~25 C Molecular mass average: 6174 ph value (50g/L,25 ): 5.1 Polyethylene glycol 8000 (PEG 8000) IMP grade GJDH17147-010 500 g HOCH 2 CH 2 (OCH 2 CH 2 ) n OH MW about 8000 Cas # 25322-68-3 Store: 18~25 C Molecular mass average: 8703.4 ph value (50g/L,25 ): 5.4 Potassium hydroxide (Caustic potash) AR grade GJDH01763-028 500 g KOH MW 56.11 Cas # 1310-58-3 Assay: 91.2% Carbonate: 0.34% Chloride: 0.005% Sulfate: 0.004% Iron: 0.0005% Total nitrogen: 0.0005% Phosphate: 0.003% Silicate: 0.01% Sodium: 0.18% Aluminium: 0.003% Calcium: 0.003% Nickle: 0.0004% Heavy metals(as Pb): 0.001% Potassium acetate AR grade C 2 H 3 O 2 K MW 98.14 Cas # 127-08-2 Assay: 97.8% ph value (450g/L,25 ): 7.0 Chloride: 0.0018% Sulfate: 0.0015% Sodium: 0.09% GJDH01730-010 500 g Phosphate: 0.0015% Calcium: 0.001% Magnesium: 0.0004% Iron: 3 ppm Heavy metals: 3 ppm Substances reducing potassium permanganate: Passes test Insoluble matter in water: 0.005% Appearance of solution: Passes test Potassium bicarbonate (Potassium hydrogen carbonate) AR grade KHCO 3 MW 100.12 Cas # 298-14-6 Assay: 99.9% Magnesium: 0.0009% Calcium: 0.001% Silicate: 0.003% Sodium: 0.015% Phosphate: 0.001% Heavy metals: 3 ppm Aluminium: 0.001% Iron: 3 ppm GJDH01765-020 500 g Chloride and chlorate: 0.004% ph value (50g/L,25 ): 8.4 Sulfur compounds: 0.003% Total nitrogen: 0.0005% Insoluble matter in water: 0.002% Appearance of solution: Passes test GeneMark 120

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Potassium carbonate anhydrous AR grade Cat. No. GJDH01860-020 Size 500 g K 2 CO 3 MW 138.21 Cas # 584-08-7 Assay: 99.7% Copper: 1 ppm Chloride: 0.002% Lead: 1 ppm Sodium: 0.013% Total nitrogen: 5 ppm Calcium: 0.0004% Magnesium: 0.0001% Iron: 2 ppm Phosphate and silicate: 0.004% Potassium chloride Sulfur compounds: 0.002% Loss on drying: 0.17% Insoluble matter in water: 0.005% AR grade KCl MW 74.55 Cas # 7447-40-7 Assay: 99.9% Barium: 0.001% Iodide: 0.001% Sulfate: 0.001% Sodium: 0.004% Phosphate: 0.0005% Calcium: 0.002% Magnesium: 0.00004% Iron: 0.00009% Heavy metals (as Pb): 0.0003% Total nitrogen: 0.0005% Bromide: 0.01% GJDH01704-040 1 kg Insoluble matter in water : 0.005% Appearance of solution : Passes test ph value (50g/L,25 ): 5.9 Potassium dihydrogen phosphate (Potassium phosphate, monobasic, anhydrous) AR grade GJDH01863-020 GJDH01701-015 1 kg 500 g KH 2 PO 4 MW 136.09 Cas # 7778-77-0 Assay: 99.5% Sodium: 0.0056% Chloride: 5 ppm Sulfate: 0.002% Iron: 5 ppm Loss on drying: 0.1% Arsenic: 2 ppm ph value (50g/L,25 ): 4.2 Total nitrogen: 7 ppm Heavy metals (as Pb): 5 ppm Insoluble matter in water: 0.001% Potassium nitrate AR grade KNO 3 MW 101.10 Cas # 7757-79-1 Assay: 99.3% Total chlorine: 0.002% ph value: 5.4 Sulfate: 0.002% Iodate: 3 ppm Manganese: 0.00002% Ammonium: 7 ppm Phosphate: 3 ppm Nitrate: 5 ppm Calcium: 0.003% Iron: 1 ppm Appearance of solution: Passes test Sodium: 0.0046% Heavy metals (as Pb): 3 ppm Insoluble matter in water: 0.004% Potassium phosphate dibasic AR grade K 2 HPO 4 3H 2 O MW 228.22 Cas # 7758-11-4 Assay: 99.9% Arsenic: 0.3 ppm Chloride: 0.001% Sulfate: 0.008% Nitrate: 8 ppm Magnesium: 1 ppm Iron: 3 ppm ph value (50g/L,25 ): 9.4 Sodium: 0.01% GJDH01862-010 1 kg Insoluble matter in water: 0.01% Heavy metals (as Pb): 3 ppm Potassium sulfate AR grade K 2 SO 4 MW 174.3 Cas # 7778-80-5 Assay: 99.8% ph value(50g/l,25 ): 6.4 Chloride: 3.5 ppm Sodium: 0.029% Calcium: 0.01% Total nitrogen: 4 ppm Arsenic: 0.5 ppm Iron: 1 ppm GJDH01702-010 500 g Insoluble matter in water: 0.005% Heavy metals (as Pb): 3 ppm GeneMark 121

Biochemicals&Solutions Biochemicals & Solutions Biochemicals Riboflavine (Vitamin B2, Vitamin G, Lectoflavine, Lectochrome) BR grade Cat. No. GJDH24429-010 Size 100 g C 17 H 20 N 4 O 6 MW 376.37 Cas # 83-88-5 Assay: 98.6% Specific optical rotation : -130 Residue after ignition: 0.04% Loss on drying: 0.44% Dimethylisoalloxazine: Passes test Salicylic acid (2-Hydroxybenzoic acid) AR grade C 7 H 6 O 3 MW 138.12 Cas # 65-45-2 Assay: 99.9% Residue after ignition: 0.007% Chloride: 0.001% Iron: 2 ppm Sulfur compounds: 0.0005% Heavy metals (as Pb): 3.5 ppm GJDH14139-010 250 g Substances darkened by sulfuric acid: Passes test Melting point: 159.5~159.7 Sodium acetate AR grade GJDH01545-020 1 kg C 2 H 3 NaO 2 MW 82.03 Cas # 127-09-3 Assay: 99.1% ph value: 8.3 Chloride: 0.001% Sulfate: 0.002% Calcium: 0.003% Magnesium: 1 ppm Aluminium: 7 ppm Iron: 3 ppm Phosphate: 3 ppm Heavy metals (as Pb): 5 ppm Insoluble matter in water: 0.003% Sodium biocarbonate (Sodium hydrogen carbonate) AR grade NaHCO 3 MW 84.01 Cas # 144-55-8 Assay: 99.9% ph value: 8.0 Sulfate: 0.003% Chloride: 0.0015% Calcium: 0.0038% Magnesium: 0.0006% Total nitrogen: 0.0006% Phosphate and silicate: 0.004% Potassium: 0.0005% Heavy metals (as Pb): 0.0007% Iron: 0.0006% GJDH01398-020 500 g Insoluble matter in water: 0.01% Appearance of solution: Passes test Sodium borate decahydrate (Sodium tetraborate; Disodium tetraborate) AR grade GJDH01398-030 500 g Na 2 B 4 O 7 10H 2 O MW 381.37 Cas # 1303-96-4 Assay: 99.9% Chloride: 0.001% Sulfate: 0.005% Phosphate: 0.001% Iron: 1 ppm Calcium: 0.0002% Copper: 0.2 ppm Lead: 0.2 ppm Insoluble matter in hydrochloric acid: 0.005% Sodium carbonate AR grade GJDH01500-030 500 g Na 2 CO 3 MW 105.99 Cas # 497-19-8 Assay: 99.8% Loss on drying: 0.25% Chloride: 0.0015% Aluminium: 0.002% Iron: 3 ppm Nitrogen compounds: 6 ppm Magnesium: 0.0017% Sulfur compounds: 0.005% Phosphate and silicate: 0.004% Potassium: 0.0001% Calcium: 0.0025% Heavy metals (as Pb): 4 ppm Insoluble matter in water: 0.01% GeneMark 122

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Sodium chloride AR grade Cat. No. GJDH01307-041 Size 1 kg NaCl MW 58.44 Cas # 7647-14-5 Assay: 99.9% Loss on drying: 0.29% ph value: 5.4 Arsenic: 0.00004% Bromide: 0.005% Total nitrogen: 0.0007% Sulfate: 0.002% Barium: 0.0007% Iodide: 0.001% Magnesium: 0.0002% Calcium: 0.0013% Potassium: 0.0004% Iron: 0.00012% Ferrocyaride: 0.0007% Phosphate: 0.0005% Heavy metals (as Pb): 0.0003% GJDH01307-045 5x1 kg Insoluble matter in water: 0.005% Appearance of solution: Passes test Sodium dihydrogen phosphate anhydrous (Sodium phosphate, monoabasic, anhydrous) AR grade GJDH01645-010 1 kg NaH 2 PO 4 MW 119.98 Cas # 7558-80-7 Assay: 99.9% ph value: 4.4 Chloride: 0.005% Nitrogen compounds: 0.0002% Heavy metals: 0.001% Sulfate: 0.01% Arsenic: 0.0002% Iron: 0.004% Insoluble matter in water < 0.01% Substances precipitated by ammonia: 0.01% Sodium hydroxide AR grade GJDH01394-068 1 kg NaOH MW 40.0 Cas # 1310-73-2 Assay: 98.3% Carbonate: 0.54% Chloride: 0.005% Sulfate: 0.003% Iron: 0.0005% Total nitrogen: 0.0008% Silicate: 0.008% potassium: 0.05% Calcium: 0.007% Phosphate: 0.0005% Aluminium: 0.001% Heavy metals (as Pb): 0.002% Sodium nitrate (Caliche; Soda niter) AR grade NaNO 3 MW 84.99 Cas # 7631-99-4 Assay: 99.5% ph value: 5.6 Iodate: 5 ppm Sulfate: 0.003% Nitrite: 5 ppm Total chlorine: 0.0015% Ammonium: 0.002% Phosphate: 5 ppm Iron: 1 ppm Potassium: 0.0015% Calcium: 0.0005% Heavy metals (as Pb): 5 ppm GJDH01304-015 500 g Insoluble matter in water: 0.004% Appearance of solution: Passes test Sodium sulfate anhydrous AR grade GJDH01509-010 1 kg Na 2 SO 4 MW 142.04 Cas # 7757-82-6 Assay:99.8% ph value: 5.8 Loss on ignition: 0.16% Chloride: 0.0005% Iron: 0.0003% Total nitrogen: 0.0003% Phosphate: 0.0007% Potassium: 0.0002% Calcium: 0.002% Heavy metals (as Pb): 0.0003% Insoluble matter in water: 0.005% GeneMark 123

Biochemicals&Solutions Biochemicals & Solutions Biochemicals Starch Soluble AR grade Cat. No. GJDH24255-010 Size 500 g (C 6 H 10 O 5 ) n (MW 164.14) n Cas # 9005-84-9 Loss on drying: 12.7% ph value: 6.6 Solubility in water: Passes test Sensitivity to iodine: Passes test Residue after ignition: 0.5% Substances reducing fehling reagent: 0.7% Sucrose AR grade C 12 H 22 O 11 MW 342.30 Cas # 57-50-1 Store: 18~25 C Loss on drying: 0.022% Sulfate: 0.0015% Chloride: 3 ppm Iron: 0.3 ppm Heavy metals (as Pb): 0.5 ppm Residue after ignition: 0.01% Acidity: 0.03 mmol/100 g GJDH18302-011 GJDH18302-015 1 kg 5x1 kg Insoluble matter in water: 0.002% Specific optical rotation: +66.2 Reducing sugars: Passes test Appearance of solution: Passes test TRICINE (N-Tris (Hydroxymethyl) methylglycine) High Purity grade GMSH0025-0500 500 g C 6 H 13 NO 5 MW 179.20 Cas # 5704-04-1 Assay: 100.1% Solubility (1 mol/l): Clear Pb: 3 ppm ph (1 M solution in water, 20 ): 5.0 UV absorbance (260nm; 280 nm): 0.028; 0.017 Loss on drying (105, 3 hours): 0.12% White crystalline powder. Soluble in water. Suitable for cell culture. TRIS (Tris (hydroxymethyl)aminoethane ) Biotech grade GMSH0026-1000 GMSH0026-5000 1 kg 5x1 kg C 4 H 11 NO 3 MW 121.14 Cas # 77-86-1 Assay: 99.98% ph (1.0% in water,25 ): 10.4 ASH: 0.008% MP/ : 168.2~170.3 Cl: 1 ppm Heavy metals: 1 ppm UV absorbance (260 nm): 0.07 Fe: 0.5 ppm Insolubles: 0.007% Water content: 0.07% White crystalline powder. Tris Acetate (Tris (hydroxymethyl) aminomethane acetate) Ultra Pure grade GMSH0027-0500 500 g C 4 H 11 NO 3. C 2 H 4 O 2 MW 181.19 Cas # 6850-28-8 Assay 99.5% Water Content: 0.5 ph (1.0% aqueous/25 ): 6.7 White crystalline powder. Solubility (1% aqueous): Clear, colorless solution TRIS-HCL (Tris(hydroxymethyl)aminomethane hydrochloride) Biotech grade GMSH0028-0500 GMSH0028-1000 GJDH22256-021 GJDH22256-025 500 g 1 kg 1 kg 5x1 kg C 4 H 11 NO 3. HCl MW 157.60 Cas # 1185-53-1 Assay: 100% Residue on Ignition: 0.02% MP: 152 ph (1.0 mol/l): 4.5 Pb: 3 ppm Solubility (1 mol/l): Clear pka(20 ): 8.28 Moisture: 0.12% Iron: 0.7 ppm UV absorbance (260 nm): 0.016 Loss on drying (105, 3 hours): 0.14% 124 GeneMark

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Biochemicals Tween 20 (Polyoxyethylene-20-sorbitan Monolaurate) CP grade C 58 H 113 O 26 MW - Cas # 9005-64-5 Density (20 C): 1.11g/ml Cat. No. Size Solubility test in ethanol: Passes test Saponification value (KOH): 49.2mg/g GJDH25301-010 500 ml Residue after ignition (as sulfate): 0.25% Acid value (KOH): 1.6mg/g Urea (Carbamide, Carbonyldiamide) AR grade GJDH20601-011 GJDH20601-015 1 kg 5x1 kg CH 4 N 2 O MW 60.06 Cas # 57-13-6 Assay: 99.2% Chloride: 1 ppm Sulfate: 6 ppm Ammonia: 0.003% Iron: 1 ppm Biuret: 0.1% Heavy metals (as Pb): 1 ppm Residue after ignition: 0.006% Insoluble matter in water: 0.005% X-Gal (BCIG; 5-Bromo-4-chloro-3-indolyl-beta-D-galactospyranoside) Ultra Pure grade GMSJ0025-1000 GMSJ0025-5000 GMSJ0028-0100 GMSJ0028-0500 GMSJ0028-1000 1 g 5 g 100 mg 500 mg 1 g C 14 H 15 BrClNO 6 MW 408.64 Cas # 7240-90-6 Assay: 99.68% Moisture Content: 0.22% Purity: 99.67% Specific Optical Rotation: -61.30 Thin Layer Chromatograph: Single Spot The IR spectrum of sample is concordant with that of working standard. White powder. Soluble in Dimethylformamide; clear, colorless solution. X-GLUC Ultra Pure grade (5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt) C 14 H 13 BrClNO 7. C 6 H 13 N MW 521.8 Cas # 114162-64-0 Purity: 99.2% Water Content: 0.08% 1H-NMR: Pass Optical rotation: -88.6 White powder. Solubility: Clear, colorless (sol 2% DMF) Methanol: 4.0% w/w Zeatin (6-(4-Hydroxy-3-methylbut-2-enylamino) purine) BR grade GJDH24445-010 50 mg C 10 H 13 N 5 O MW 219.24 Cas # 1637-39-4 Assay: 99.2% Loss on ignition: 0.036% Loss on drying: 3.0% Character: White powder Melting Range: 210.3 125 GeneMark

Biochemicals&Solutions Biochemicals & Solutions Ready to Use Buffers Product Name Product composition Ordering Info 50X TAE Buffer (Sterile) TAE buffer is the most commonly used electrophoresis buffer for agarose gels. It has a lower buffer capacity than TBE, but double-stranded linear DNA migrates 10% faster with the same resolution through TAEcontaining agarose gels. The working concentration is 1X or 0.5X TAE at room temperature. 2 M Tris-acetate, 50 mm EDTA-Na, ph 8.5 GB01-1, 1L GB01-4, 4 x 1 L 5X TBE Buffer (Sterile) TBE buffer is the electophoresis buffer for polyacrylamide gels and agarose gels. TBE has a higher buffering capacity than TAE. Normaly, it is applied in a concentration of 1X for polyacryl-amide gels and 0.5X for agarose gels and "band shifts" (gel mobility shift assay). TBE is prepared as a 5X concentrate and stored at room temperature. 0.445 M Tris, 0.01 M EDTA-Na, 0.445 M Boric acid, ph 8.3 GB02-1, 1L GB02-4, 4 x 1 L 10X TG Buffer (Tris-Glycine Buffer, sterile) TG buffer is the electophoresis buffer for polyacrylamide gels, which does not include SDS for protein denature in PAGE. TG Buffer is also use for transfer of Western blot. The buffer is prepared as a 10X concentrate and stored at room temperature. 0.25 M Tris, 1.92 M Glycine, ph 8.3 GB04-1, 1L GB04-4, 4 x 1 L 10X TGS Buffer (Tris-Glycine-SDS Buffer, sterile) TGS buffer is the electophoresis buffer for polyacrylamide gels, which includes SDS for protein denature in the PAGE. TGS buffer is prepared as a 10X concentrate and stored at room temperature. 0.25 M Tris, 1.92 M Glycine, 10% SDS, ph 8.3 GB05-1, 1L GB05-4, 4 x 1 L 10X TE Buffer (Tris-EDTA Buffer, sterile) TE-buffer (ph 8.0) is the standard buffer solution for dissolving and storage of plasmid DNA or oligonucleotides, because nucleic acids are largely protected from degradation. TE buffer is prepared as a 10X concentrate and stored at room temperature. 10mM Tris, EDTA-Na.H 2 O, ph 8.0 GB06-1, 1L 10X TEN Buffer (Sterile) TEN buffer can be used as lysis buffer for eukaryotic cells, especially in the alkaline lysis protocol for the M13 isolation. 0.4 M Tris, 10 mm EDTA-Na.H 2 O, 1.5 M Sodium Chloride, ph 7.5 GB10-1, 1L 10X PBS Buffer (Phosphate Buffered Saline, sterile) PBS buffer is commonly used in biological researches such as cell culturing. The buffer is prepared as a 10X concentrate and stored at room temperature. 1.37 mm Sodium Chloride, 27 mm Protassium Chloride, 43 mm Na 2 HPO 4, 14 mm KH 2 PO 4, ph 6.75 GB07-1, 1L GB07-4, 4 x 1 L 10X TBS Buffer (Tris Buffered Saline, sterile) TBS buffer is prepared as a 10X concentrate and stored at room temperature and is used in Western Blot for protein detection. 0.25 mm Tris, 1.37 M Sodium Chloride, 0.027 M Protassium Chloride ph 7.4±0.05 GB08-1, 1L 126 GeneMark

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Ready to Use Buffers Product Name Product composition Order Info 10X TBST Buffer (Tris Buffered Saline Tween 20) 10X TBST buffer is prepared as a 10X concentrate and is stored at room temperature. This buffer is used in Western Blot for protein detection. 0.25 MTris, 1.37 M Sodium Chloride, 0.027 M Protassium Chloride, 1% Tween 20 GB08T-1, 1L 20X SSPE Buffer Detection of specific sequences of DNA fragments after gel electrophoresis. 3 M Sodium chloride, 0.2 M NaH 2 PO 4.H 2 O, 0.02 M EDTA-Na.H 2 O, ph 7.4 GB09-1, 1L GB09-4, 4 x 1 L 20X SSC Buffer SSC buffer can be used in nucleic acid hybridization techniques and for detection of specific sequences of DNA-fragments after gel electrophoresis. 3 M Sodium chloride, 0.3 M tri-sodium citrate, ph 7.0 GB03-1, 1L GB03-4, 4 x 1 L 6 x DNA Loading Dye The GeneMark 6X DNA Loading Dye is used for DNA electrophoresis in agarose gel. Add it directly to DNA sample in 1X final concentration, then load to the gel. 150 mm EDTA, 30% Glycerol, 0.1% Bromophenol Blue, 0.1% Xylene cyanol F.F. DL02-1 DL02-100 5 x Protein Loading Buffer GeneMark 5X Protein Sample Buffer is especially formulated for use in protein sample preparations for SDS-PAGE system. A protein sample is mixed with the 5X sample buffer (4:1) and is boiled (or heated) on a heating block for 2~5 minutes. 10% SDS, 50% glycerol, 0.05 M DTT, 0.01 M EDTA, 0.05% bromophenol blue and 0.125 M Tris HCl, ph approx. 6.8. GM47-b-50 GM47-b-100 InstantBlue Gel Staining Reagent InstantBlue TM Gel Staining Reagent is a convenient alternative to traditional Coomassie Blue staining procedures, based on a colloidal G250 formulation. This ready-to-use stain contains no methanol, acetic acid and TCA for staining and requires no hazardous solvents for destaining. Protein bands analyzed using polyacrylamide gels are visible directly during the staining process just in 3 minutes. After staining, a simple and quick washing with water to yield a clear background, let sensitivity down to 8 ng under standard procedure. The simple procedure saves valuable time by reducing the handling of hazardous materials and solvent waste in the laboratory. 8 ng 1 / 50 1 / 20 1 / 10 1 / 5 1 / 2 3 μl GM49 127 GeneMark

Biochemicals&Solutions Biochemicals & Solutions RNA related solution Product Name Ordering Info RNase DNase Away Solution Ensure your PCR workstations, countertops and apparatus are free from DNase or RNase contamination by using RNase DNase Away Solution. This specially formulated surface decontaminant is designed to eliminate all DNase or RNase from glassware without having a residual effect on subsequent DNA samples. In fact, RNase DNase Away Solution is proved to be more effective at degrading DNase than autoclaving! Simply apply RNase DNase Away Solution to the surfaces or lab wares to decontaminate and rinse clear. Storage: RT D0339-100, 100 ml D0339-500, 500 ml RNase A stock (DNase-free) RNA- SafeGuard Reagent (20X) Ribonuclease A (RNase A) is an endoribonuclease, that specifically cleaves single-stranded RNA 3' to pyrimidine residues (cytosine, uracil). Thereby, it generates pyrimidine-3'-phosphate or oligonucleotides with terminal pyrimidine-3'-phosphates. The phoptimum is in the range of 7.0~7.5. RNase A is used for the purification of RNase-free DNA, for the removal of non-hybridized regions of RNA:DNA-hybrids or as a molecular weight marker. RNase A stock are pre-pared at concentrations 4 or 10 mg/ml. Storage: -20 ºC RNA-SafeGuard is a mixture of non-toxic reagents for storage and decontamination of RNase for purified RNA. RNA stored in this reagent can be used in many enzymatic reactions, including cdna synthesis, RT-PCR and in vitro transcription. RNA-SafeGuard can also be used for preparation of molecular buffer instead of DEPC, or used for buffer incompatible with DEPC, or solutions that cannot be autoclaved. Storage: -20 RA01: 4 mg/ml, 4 ml RA02: 10 mg/ml, 4 ml RG-1, 1 ml RG-10, 10 ml Figure 1. RT-PCR of rat beta-actin from RNA stored in different solutions. Total RNA was isolated from rat spleen, and RNA was resuspended in RNase-free water. One third of RNA treated with 1X RNASecure, one third of RNA treated with 1X RNA-SafeGuard. RT-PCR was performed using One-Step RT-PCR kit (CAT# RP01) with expected PCR product to be 513 bp. Lane 1: RNase-free water Lane 2: Ambion RNASecure Lane 3: RNA-SafeGuard (-) : negative control, RNA in RNAase-free water with Taq only 128 GeneMark

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions RNA related solution Product Name Order Info RNAfter TM RNAfter TM is a non-toxic reagent for storage of various animal or plant tissues, cultured cells and bacteria for RNA purification without using liquid nitrogen or -70 freezer. The sample can be stored in RNAfter TM reagent for a day at 37, 1 week at RT, one month at 4 and indefinitely at -20. The purified RNA quality is as high as stored in liquid nitrogen. Features: 1. Simple and Stable: Intact RNA was recovered from that was stored in RNAfter TM at any temperature condition. Storage : RT Note: This reagent can be used in genomic DNA purification from various samples. RA-100, 100 ml RA-500, 500 ml Figure 1. RNA purified from rat kidney stored in RNAfter TM using TriSolution. 1: Stored at 37 for 24h in RNAfter TM 2: Stored at 4 for 30 days in RNAfter TM 3: Stored at -70 for 30 days without RNAfter TM GeneMark 129

Biochemicals&Solutions Biochemicals & Solutions Nucleic Acid Stain Solution Product Name Ordering Info Ethidium Bromide Solution SafeView DNA Stain Ethidium bromide is the most commonly used dye for staining DNA during or after polyacrylamide or agarose gel electrophoresis. Excitation with light at a wave length of 254 nm is absorbed by the DNA and transferred to ethidium bromide. Excitation at 366 nm directly excites the dye. An aqueous stock solution is prepared at a concentration of 10 mg/ml and employed at 0.2~0.5 μg/ml. Staining is performed after the gel run (especially PAGE) or during the gel run (agarose). The latter allows the observation of the gel run by control under UV light. Ethidium bromide is added to the agarose solution. Please note, that "prestaining" may lead to artifacts. Storage: Store the stock solution at 4, prevent direct light exposure. SafeView TM is a new, safe nucleic acid stain for detection of double-stranded DNA, single-stranded DNA and RNA in agarose gels. This dye replaces ethidium bromide (a potent mutagen and toxic) for visualization of DNA or RNA in agarose gel. SafeView TM is non-carcinogenic, is as sensitive as ethidium bromide (EB) and is used the same way as EB in agarose gel electrophoresis. SafeView TM emits green fluorescence when bound to dsdna and red fluorescence when bound to ssdna or RNA. Storage: 4 D0197 (10 mg/ml, 5 ml) C118-1, 1 ml C118-5, 5 ml GeneMark 130

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions dntp Solution Sets 2.5 mm / 10 mm dntp Solution Set The dntps are preneutralized and ready for immediate use in PCR. The solution contains 2.5 mm of each dntp (datp, dctp, dgtp and dttp) for 2.5 mm dntp Mix, and 10 mm of each dntp ( datp, dctp, dgtp and dttp) for 10 mm dntp Mix. dntps are adjusted to ph 7.0 with NaOH to provide in PCR. M 1 2 3 Figure 1. Amplification of Lamba DNA fragments using 2.5 mm dnpt Mix. Lane 1: 5 μl PCR Product of 6 kb Lane 2: 5 μl PCR Product of 8 kb Lane 3: 5 μl PCR Product of 14 kb 14 kb 8 kb 6 kb 100 mm dntp (A,C,G,T) / dntp (A,C,G,U) Solution Set Separate vials of datp, dgtp, dcpt and dttp each at a concentration of 100 mm for dntp (A,C,G,T) Solution Set; and separate vials of datp, dgtp, dctp and dutp each at a concentration of 100 mm for dntp (A,C,G,U) Solution Set. The dntps are made by dissolving ultra-pure triphosphate powders in buffer at ph 7.0. M 1 2 3 14 kb 8 kb 6 kb Figure 2. Amplification of Lamba DNA fragments using 100 mm dnpt Mix. Lane 1: 4 μl PCR Product of 6 kb Lane 2: 4 μl PCR Product of 8 kb Lane 3: 4 μl PCR Product of 14 kb GeneMark 131

Biochemicals&Solutions Biochemicals & Solutions Ordering Info DESCRIPTION Cat.No. SIZE 2.5 mm dntp(a, C, G,T) each, Solution Set GM0002 1 ml 10 mm dntp(a, C, G,T) each, Solution Set GM007 1 ml 100 mm dntp(a, C, G, T) Solution Set GD002-ACGT 400 μl each 100 mm dntp(a, C, G,U) Solution Set GD002-ACGU 400 μl each 100 mm datp Solution Set GD002-A 400 μl 100 mm dctp Solution Set GD002-C 400 μl 100 mm dgtp Solution Set GD002-G 400 μl 100 mm dttp Solution Set GD002-T 400 μl 100 mm dutp Solution Set GD002-U 400 μl GeneMark 132

GeneMark GMbiolab Co., Ltd. Biochemicals&Solutions Biochemicals & Solutions Antibiotic Solutions Ordering Info DESCRIPTION Cat.No. Qty Stock Conc. Sterillity Amphotericin B GAS01 1 ml x 10 2.5 mg/ml in H 2 O 0.22 µm filtered Ampicillin GAS02 1 ml x 10 100 mg/ml in H 2 O 0.22 µm filtered Carbenicillin GAS03 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Cefotaxime GAS04 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Chloramphenicol GAS05 1 ml x 10 50 mg/ml in 100% EtOH need not be filtered Erythromycin GAS06 1 ml x 10 10 mg/ml in H 2 O 0.22 µm filtered G 418 GAS07 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Gentamicin GAS08 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Hygromycin B GAS09 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Kanamycin GAS10 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Neomycin GAS11 1 ml x 10 10 mg/ml in H 2 O 0.22 µm filtered Penicillin-G GAS12 1 ml x 10 10,000 U/ml in H 2 O 0.22 µm filtered Rifampicin GAS13 1 ml x 10 30 mg/ml in DMSO need not be filtered Spectinomycin GAS14 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Streptomycin GAS15 1 ml x 10 50 mg/ml in H 2 O 0.22 µm filtered Tetracyline GAS16 1 ml x 10 5 mg/ml in H 2 O 0.22 µm filtered Antibiotic Antimycotic Solution (100x) GAS011213 100 ml 10,000 units penicillin, 10 mg streptomycin, 25 µg amphotericin B per ml in H 2 O 0.22 µm filtered Penicillin-Streptomycin Solution GAS1213 100ml 10,000 units penicillin-g, 10 mg streptomycin per ml in H 2 O 0.22 µm filtered Penicillin-Streptomycin- Neomycin solution GAS111213 100ml 5,000 units penicillin-g, 5 mg streptomycin, 10 mg neomycin per ml in H 2 O 0.22 µm filtered GeneMark 133

Biochemicals&Solutions Biochemicals & Solutions Other Solution Product Name Ordering Info Denhardt's Solution (100 X) Glycogen Solution Denhardt's solution is a blocking reagent for preventing nonspecific binding of nucleic acids to nitrocellulose or nylon membranes in hybridization experiments. Denatured DNA binds to nitrocellulose membranes, but not free RNA. Pretreatment of the membrane with Denhardt's solution prevents the binding of single stranded or (nonspecific) denatured DNA. The specific annealing of denatured DNA to its complementary DNA is not inhibited. The use of Denhardt's reagent is recommended for Northern hybridization, hybridization of RNA, "single copy Southern hybridization" or hybridization of DNA immobilized on nylon membranes. Denhardt's solution is set up as a 100X stock solution (100X reagent: 2% Ficoll 400, 2% polyvinylpyrolidone, 2% BSA fraction V in water). Storage: Filtered and stored at -20. Nanogram quantities of DNA can be efficiently precipitated with the help of "carriers" (glycogen, yeast-trna). Glycogen can be applied when nucleic acids are precipitated with 0.5 M ammonium acetate and isopropanol. Since glycogen is not a nucleic acid, it does not interfere with enzyme reactions as yeast-trna may do. But it may interfere with the interactions between proteins and DNA. The working concentration is approximately 50 μg/ml of glycogen. Concentration: 20 mg/ml Storage: -20 GM006-10, 10 ml GM006-50, 50 ml GM14 (8 x 0.5 ml ) Salmon Sperm DNA Solution Salmon sperm DNA is added to the pre-hybridization solution to prevent unspecific binding of probes to the membrane in Southern and Northern-hybridization. Salmon sperm DNA is sonified (fragments of 500~1000 base-pair) and extracted with phenol chloroform. The lyophilised DNA is dissolved in sterile bidistilled water. This DNA solution is used in a final concentration of 100 μg/ml. Concentration: 10 mg/ml Storage: -20 SS01 (8 x 1 ml) Red Cell Lysis Buffer Red Cell Lysis buffer contains ammonium chloride to efficiently lyse red blood cells. Add 3 times the sample volume of buffer and place at room temperature for 5 minutes to lyse, and centrifuge at 10000 rpm for 1 minute to obtain cell pellet. GRT122-01, 100 ml GRT122-02, 250 ml GeneMark 134

GeneMark GMbiolab Co., Ltd. Plastic Lab Wares Tubes and Tips 15 ml Conical Centrifuge Tubes 0.1~10 μl Clear tips/filter Tips 50 ml Conical Centrifuge Tubes 1~200 μl Yellow tips/ Filter Tips 1.5 ml Microcentrifuge Tubes 50~1000 μl Blue tips/ Filter Tips 2.0 ml Microcentrifuge Tubes 0.2 ml PCR Thin Wall Tubes Racks and Freezer Boxes 0.5~1.5 ml, 80 Well Reversible Microtube Rack 0.5~1.5 ml, 96 Well Reversible Microtube Rack 4-Ways Microtube & Centrifuge Tubes Racks 0.2 ml PCR Rack Freezer Box with 9 x 9 Divider Freezer Box with 10 x 10 Divider 96 Deep Well Plates and Films 1.6 ml, 96 Deep Well Plates, Square, Individual Pack, Sterile 2.2 ml, 96 Deep Well Plates, Square, Individual Pack, Sterile 96 Well Silicone Films Film for 96 Deep Well 135 GeneMark

Plastic Lab Wares Plastic Lab Wares Tubes 15 ml / 50 ml Conical Centrifuge Tubes Blue printed graduation for accurate input with better toleration to physical stress Leakproof screw cap design, with easy one-hand operation! Clear tube, suitable for most rotors! Capable for centrifugation rated to 8,000 xg ~ 9,500 xg! Withstands temperature from -80 ~120 and autoclavable! DNase/RNase free, sterile 15 ml Conical Centrifuge Tubes 50 ml Conical Centrifuge Tubes Ordering Info DESCRIPTION Cat.No. Package 15 ml PP, conical, Printed Sterile, 25/bag 23-2266 500/cs 15 ml PP, conical, Printed Sterile, 50/rack 23-2265 500/cs 50 ml PP, conical, Printed Sterile, 25/bag 23-2263 500/cs 50 ml PP, conical, Printed Sterile, 50/rack 23-2262 500/cs GeneMark 136

GeneMark GMbiolab Co., Ltd. Plastic Lab Wares Plastic Lab Wares Tubes 1.5 ml / 2.0 ml Microcentrifuge Tubes, Large cap Flat-Top graduated clear tubes for easy labeling Large cap to allow easy one-hand opening and closing Capable for centrifugation rated to 14000 rpm Acid-alkali tolerant, autoclavable and can be used with organic solvents Non-pyrogenic, RNase/ DNase free 0.2 ml PCR Thin Wall Tubes Thin walls for optimal thermal conductivity and uniform heat distribution Great hardness to prevent shatter from centrifugation Flat-Top for easy labeling RNase/ Dnase-free Suitable for PCR machines of all brands Ordering Info DESCRIPTION Cat.No. Package 1.5 ml Microcentrifuge Tube, large cap L-2057 2.0 ml Microcentrifuge Tube XL-2911 0.2 ml PCR Thin Wall Tubes GM02PCR 500/bag, 10 bags/case 500/ bag, 10 bags/case 1000/bag, 10 bags/case 137 GeneMark

Plastic Lab Wares Plastic Lab Wares Tips and Tip Racks Standard Tips PP material, graduated clear tips Suitable for pipeteman of all brands DNase, RNase free Low retention L-4999 L-2346 S-5109 Filter Tips PP material graduated clear tips Suitable for pipetman of all brands DNase, RNase free Low retention Sterile, ready-to-use L-1150 S-5106 Ordering Info DESCRIPTION Cat.No. Package S-5107 0.1~10 μl Clear tip, 1000/bag, DNase, RNase free L-4999 10000/cs 1~200 μl Yellow tip, 1000/bag, DNase, RNase free L-2346 10000/cs 50~1000 μl Blue tip, 1000/bag, DNase, RNase free L-1150 10000/cs 0.5~10 μl Tip Rack R-4999 1 rack 1~200 μl Tip Rack R-2346 1 rack 100~1000 μl Tip Rack R-1150 1 rack 0.1~10 μl Extra long Filter Tip, 96/rack, DNase, RNase free S-5109 10 rack/cs 1~200 μl Filter Tip, 96/rack, DNase, RNase free S-5106 10 rack/cs 50~1000 μl Filter Tip, 96/rack, DNase, RNase free S-5107 10 rack/cs GeneMark 138

GeneMark GMbiolab Co., Ltd. Plastic Lab Wares Plastic Lab Wares Racks 80 Well / 96 Well Microtube Racks & 0.2 ml PCR rack PP material. For 96 well microtube racks, each side fits 80 x 1.5 ml or 80 x 0.5 ml tubes; or 96 x 1.5 ml or 96 x 0.5 ml. Cover is available. For 0.2 ml PCR rack, rack fits 96 x 0.2 ml PCR tubes and is autoclavable. Cover is supplied. R567-M R577-M R577-M + R577-C GMI607 4-Ways Microtubes & Centrifuge Tubes Rack PP material. The rack holds 4 x 50 ml centrifuge tubes, 12 x 15 ml conical tubes, 32 x 1.5 ml (0.2 ml) tubes and 32 x 0.5 ml tubes. GMI-R023 Ordering Info DESCRIPTION Cat.No. Package 0.5~1.5 ml, 80 Well Reversible Microtube Rack R567-M 1 rack 0.5~1.5 ml, 96 Well Reversible Microtube Rack R577-M 1 rack 0.5~1.5 ml, 96 Well Reversible Microtube Rack (with cover ) R577-M +R577-C 1 rack 4-Ways Microtube & Centrifuge Tubes Racks GMI-R023 1 rack 0.2 ml PCR Rack GMI607 1 rack 139 GeneMark

Plastic Lab Wares Plastic Lab Wares Freezer Boxes Freezer Box with 9 x 9 / 10 x 10 Divider 9 x 9 and 10 x 10 dividers which hold 81 and 100 microcentrifuge tubes, respectively. For sample storage at -20 or - 80. 96 Well Plates 1.6 ml / 2.2 ml Deep Well Plates, square, Individual pack, Sterile Round bottom wells designed for high throughput and storage application PP material Sealable with 96 well Silicone film (GeneMark Cat. No.: KG4100) or Film for 96 deep well (GeneMark Cat. No.: KG4101) Notched corner design to facilitate orientation KG4101 KG4100 KG4131, KG4231 Ordering Info DESCRIPTION Cat.No. Package 1.6 ml Deep Well Plates, square, Individual pack, Sterile 2.2 ml Deep Well Plates, square, Individual pack, Sterile KG4131 KG4231 1 plate 1 plate 96 Well Silicone Film KG4100 10 psc/ bag Film for 96 Deep Well Plates KG4101 96 films/ bag Freezer Box with 9 x 9 Divider C5520-CD81 1 piece Freezer Box with 10 x 10 Divider C5520-CD100 1 piece GeneMark 140

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection Plant Virus Detection Kits Low Cost High sensitivity More Reliability 141 GeneMark

Plant Virus Detection Plant Virus Detection Plant Virus Detection Kits Product Info Plant Virus ELISA Detection RT-PCR Detection SYBR RT-qPCR Detection Antibody for Detection Orchid ORSV + + + Polyclonol antibody, 1000x CymMV + + + Polyclonol antibody, 1000x CaCV + + + Monoclonol antibody, 1000x CMV + + + Polyclonol antibody, 1000x INSV + + + Polyclonol antibody, 1000x TSWV + + + Polyclonol antibody, 1000x OFV - + + - PhCSV - + + - CarMV - + + - RT-PCR and Bamboo BaMV - NEST PCR + - Papaya PRSV + + + Polyclonol antibody, 1000x PLDMV + + + Polyclonol antibody, 1000x Melon WSMoV + + + Monoclonol antibody, 1000x MYSV + + + Monoclonol antibody, 1000x CCYV + + + Polyclonol antibody, 1000x CMV + + + Polyclonol antibody, 1000x Dragon Fruit CVX - + + - ZVX - + + - PtVX - + + - Tomato TYLCV - PCR PCR - ToLCV - PCR PCR - CMV + + + Polyclonol antibody, 1000x TSWV - + + - Banana BBTV - PCR PCR - BSV - + + - CMV + + + Polyclonol antibody, 1000x Passion Fruit PWV - + + - CMV + + + Polyclonol antibody, 1000x Flamingo Flower DsMV - + + - CMV + + + Polyclonol antibody, 1000x Ornithogalum OrMV - + + - GeneMark 142

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection Plant Virus Detection Kits Product Info Plant Virus ELISA Detection RT-PCR Detection SYBR RT-qPCR Detection Lily ArMV - + + - TRV - + + - TRSV - + + - LVX - + + - LMoV - + + - LSV - + + - Antibody for Detection CMV + + + Polyclonol antibody, 1000x Tulip TBV - + + - ArMV - + + - TRV - + + - CMV + + + Polyclonol antibody, 1000x LSV - + + - Gladiolus BYMV - + + - CMV + + + Polyclonol antibody, 1000x Arum Lily DsMV - + + - CMV + + + Polyclonol antibody, 1000x INSV - + + - CCSV - + + - BYMV - + + - ZaMV - + + - LNV - + + - TuMV - + + - Prairie Gentian BYMV - + + - BBWV - + + - LNV - + + - CMV + + + Polyclonol antibody, 1000x TuMV - + + - TSWV - + + - Product Cat # Package 10X Coating buffer for ELISA detection, 500 tests ECB-500 200 ml Antibody dilution buffer for ELISA detection, 500 tests EAB-500 250 ml Substrate and buffer set for ELIZA detection, 500 tests ESB-500 1 set * For virus detection services, please refer to page 169. 143 GeneMark

Plant Virus Detection Plant Virus Detection Features Sensitivity: Can detect viral protein particles as little as 0.025 ng/μl from plant samples. ELISA Detection Kits GeneMark provides ELISA detection kits for detection of various viruses from different plant samples. The ELISA detection kit is based on the ELISA method, that is, by detecting the change of OD 405 value due to interactions between antibody and viral protein. This method detects a single type of virus at once for each reaction, and can detect viral protein particles as little as 0.025 ng/μl. Polyclonal and/or monoclonal antibody is also available for purchase for other molecular experiments such as western blotting. Principle of Indirect ELISA Fast and economical Low cots and fast detection for large amount of samples Application Viral DNA detection Antigen-coating Primary antibody reacts with antigens Measure the change of OD 405. Color change is proportional to the amount of antigen coated. S S S S Enzyme-linked Secondary antibody binds to primary antibody Substrate is added followed by enzymatic reactions to release colored product. GeneMark 144

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection ELISA Detection Kits Ordering Info Product Name ELISA Detection Kit for Phalaenopsis (CymMV), 480 tests ELISA Detection Kit for Phalaenopsis (ORSV), 480 tests ELISA Detection Kit for Phalaenopsis (CaCV), 480 tests ELISA Detection Kit for Phalaenopsis (INSV), 480 tests ELISA Detection Kit for Phalaenopsis (TSWV), 480 tests ELISA Detection Kit for Papaya (PRSV), 480 tests ELISA Detection Kit for Papaya (PLDMV), 480 tests ELISA Detection Kit for Melon (WSMoV), 480 tests ELISA Detection Kit for Melon (MYSV), 480 tests ELISA Detection Kit for Melon (CCYV), 480 tests ELISA Detection Kit for CMV-group 1, 480 tests Cat. No. GMK01CM-480 GMK02OR-480 GMK03CA-480 GMK08INS-480 GMK09TSW-480 GMK06PRS-480 GMK07PLD-480 GMK11WSM-480 GMK12MYS-480 GMK13CCY-480 GMK04CM-480 Product Name Polyclonal antibody for detection of Phalaenopsis (ORSV), 1000X Polyclonal antibody for detection of Phalaenopsis (CymMV), 1000X Polyclonal antibody for detection of Papaya (PRSV), 1000X Polyclonal antibody for detection of Papaya (PLDMV), 1000X Monoclonal antibody for detection of Melon (WSMoV), 1000X Monoclonal antibody for detection of Melon (MYSV), 1000X Polyclonal antibody for detection of Melon (CCYV), 1000X Polyclonal antibody for detection of CaCV, 1000X Polyclonal antibody for detection of INSV, 1000X Polyclonal antibody for detection of TSWV, 1000X Polyclonal antibody for detection of CMV-group 1, 1000X Cat. No. EAb02OR EAb01CM EAb06PRS EAb07PLD EAb11WSM EAb12MYS EAb13CCY EAb03CA EAb08INS EAb09TSW EAb04CM 145 GeneMark

Plant Virus Detection Plant Virus Detection Application Viral DNA detection Ordering Info RT-PCR Detection Kits GeneMark RT-PCR/PCR Detection series provide detection kits for various plant viruses. The series is mostly based on One-Step RT-PCR method, which is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mrna. The system uses M-MLV reverse transcriptase for first-strand cdna synthesis and PCR amplification. This onetube, two enzyme system provides sensitive and quick analysis of RNA detection. As for DNA viruses, PCR is used for viral DNA amplification. Cat. No. Product Name Plant RP01-CO RP01-CA RP01-CC One-Step Multiplex RT-PCR Detection kit for CymMV & ORSV of Phalaenopsis Orchid, 100 reactions One-Step Multiplex RT-PCR Detection kit for CaCV of Phalaenopsis Orchid, 100 reactions One-Step Multiplex RT-PCR Detection kit for CMV (group-1) & CaCV of Phalaenopsis Orchid, 100 reactions Phalaenopsis Phalaenopsis Phalaenopsis RP01-CM One-Step RT-PCR Detection kit for CMV (group-1), 100 reactions Various Plants RP01-INS RP01-TSW RP01-DsM One-Step RT-PCR Detection kit for INSV, 100 reactions One-Step RT-PCR Detection kit for TSWV, 100 reactions One-Step RT-PCR Detection kit for DsMV, 100 reactions Phalaenopsis, Arum Lily Phalaenopsis, Tomato, Prairie Gentian Flamingo Flower, Arum Lily RP01-ArM One-Step RT-PCR Detection kit for ArMV of Lily, 100 reactions Lily, Tulip RP01-TRV One-Step RT-PCR Detection kit for TRV of Lily, 100 reactions Lily, Tulip RP01-LSV One-Step RT-PCR Detection kit for LSV of Lily, 100 reactions Lily, Tulip RP01-OF RP01-PhC RP01-CarM One-Step RT-PCR Detection kit for OFV of Phalaenopsis Orchid, 100 reactions One-Step RT-PCR Detection kit for PhCSV of Phalaenopsis Orchid, 100 reactions One-Step RT-PCR Detection kit for CarMV of Phalaenopsis Orchid, 100 reactions Phalaenopsis Phalaenopsis Phalaenopsis RP01-BaM One-Step RT-PCR Detection kit for BaMV of Bamboo, 100 reactions Bamboo RP01-PLD One-Step RT-PCR Detection kit for PLDMV of Papaya, 100 reactions Papaya RP01-PRS One-Step RT-PCR Detection kit for PRSV of Papaya, 100 reactions Papaya GeneMark 146

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection RT-PCR Detection Kits Ordering Info Cat. No. Product Name Plant RP01-WMC RP01-PtCZ One-Step Multiplex RT-PCR Detection kit for MYSV, WSMoV and CCYV of Melon, 100 reactions One-Step Multiplex RT-PCR Detection kit for PtMV, CVX and ZVX of Dragon Fruit, 100 reactions Melon Dragon Fruit RP01-PtM One-Step RT-PCR Detection kit for PtMV of Dragon Fruit, 100 reactions Dragon Fruit RP01-CVX One-Step RT-PCR Detection kit for CVX of Dragon Fruit, 100 reactions Dragon Fruit RP01-ZVX One-Step RT-PCR Detection kit for ZVX of Dragon Fruit, 100 reactions Dragon Fruit RP02-TYL PCR Detection kit for TYLCV of Tomato, 100 reactions Tomato RP02-ToLC PCR Detection kit for ToLCVof Tomato, 100 reactions Tomato RP02-BBT PCR Detection kit for BBTV of Banana, 100 reactions Banana RP01-BSV One-Step RT-PCR Detection kit for BSV of Banana, 100 reactions Banana RP01-PWV One-Step RT-PCR Detection kit for PWV of Passion Fruit, 100 reactions Passion Fruit RP01-OrM One-Step RT-PCR Detection kit for OrMV of Ornithogalum, 100 reactions Ornithogalum RP01-TRS One-Step RT-PCR Detection kit for TRSV of Lily, 100 reactions Lily RP01-LVX One-Step RT-PCR Detection kit for LVX of Lily, 100 reactions Lily RP01-Lmo One-Step RT-PCR Detection kit for LMoV of Lily, 100 reactions Lily RP01-TBV One-Step RT-PCR Detection kit for TBV of Tulip, 100 reactions Tulip RP01-BYM One-Step RT-PCR Detection kit for BYMV of Gladiolus, 100 reactions Gladiolus RP01-CCS One-Step RT-PCR Detection kit for CCSV of Arum Lily, 100 reactions Arum Lily RP01-ZaM One-Step RT-PCR Detection kit for ZaMV of Arum Lily, 100 reactions Arum Lily RP01-LNV One-Step RT-PCR Detection kit for LNV of Arum Lily, 100 reactions Arum Lily RP01-TuM One-Step RT-PCR Detection kit for TuMV of Arum Lily, 100 reactions Arum Lily RP01-BBW One-Step RT-PCR Detection kit for BBWV of Prairie Gentian, 100 reactions Prairie Gentian 147 GeneMark

Plant Virus Detection Plant Virus Detection One-Step Multiplex RT-PCR Detection Kit For CymMV & ORSV of Phalaenopsis Orchid Features High Sensitivity: Highly sensitive, can detect minimal amount of virus RNA (1.3 x 10-9 ng). Convenient: Can detect more than one kind of virus strain at once. Fast and Accurate: One-step RT-PCR mix containing specific primers for viral gene detection. The One-Step Multiplex RT-PCR Detection Kit For CymMV and ORSV of Phalaenopsis Orchid is designed as one-tube, two-enzyme system and multiplex virus-specific primers to provides sensitive, quick and specific analysis of CymMV (Cymbidium mosaic virus) and ORSV (Odontoglossum ringspot virus) RNA from the infected Phalaenopsis orchid. The system uses M- MLV reverse transcriptase for first-strand cdna synthesis and Taq for PCR amplification, and offers positive multiplex RT-PCR reaction. The amplified DNA fragments are generated with a 3 A-overhang that can be cloned into T-vector. Application Viral DNA amplification, cloning RNA (ng/ul) M 140 14 1.4 CymMV ORSV Orchid Ribosomal specific fragment (158bp) Figure 1. Sensitivity test of phalaenopisis Orchid virus detection using One-Step Multiplex RT-PCR Detection Kit. CymMV : Cymbidium mosaic virus ORSV : Odontoglossum ringspot virus Internal Control: Orchid ribosomal specific fragment Ordering Info DESCRIPTION Cat.No. Reaction One-Step Multiplex RT-PCR Detection kit for CymMV & ORSV of Phalaenopsis Orchid RP01-CO 100 GeneMark 148

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection One-Step Multiplex RT-PCR Detection Kit For CaCV of Phalaenopsis Orchid Features High Sensitivity: Highly sensitive, can detect minimal amount of virus RNA (1.3 x 10-9 ng). Fast and Accurate: One-step RT-PCR mix containing specific primers for viral gene detection. The One-Step Multiplex RT-PCR Detection Kit For CaCVof Phalaenopsis Orchid is designed as one-tube, two-enzyme system and CaCV multiplex specific primers to provides sensitive, quick and specific analysis of CaCV (Capsicum chlorosis virus) RNA from the infected Phalaenopsis orchid. The system uses M- MLV reverse transcriptase for first-strand cdna synthesis and PCR amplification, and offers positive control and ribosomal RNA specific primer as standard in the one-step multiplex RT-PCR reaction. The amplified DNA fragments are generated with an 3 A-overhang that can be cloned into T- vector. M-MLV reverse transcriptase lacks a 3 5 exonucleolytic proofreading function and has a weak RNase H activity compared to AMV reverse transcriptase, it is useful for full-length cdna amplification. Application Viral DNA amplification, cloning Standard Infected Infected Infected CaCV-NP (777bp) CaCV-RdRP (355bp) Orchid Ribosomal Specific Fragment (158bp) Ordering Info DESCRIPTION Cat.No. Reaction One-Step Multiplex RT-PCR Detection kit for CaCV of Phalaenopsis Orchid RP01-CA 100 149 GeneMark

Plant Virus Detection Plant Virus Detection SYBR RT-qPCR Detection Kits Application Viral DNA detection For detection of plant viral RNA/DNA, GeneMark also provides SYBR RT-qPCR Kits specific for certain plant viruses. Ordering Info Cat.No. Plant Reaction RPQ01-CM CMV (group-1) SYBR RT-qPCR Kit Various Plants 100 RPQ01-IN INSV SYBR RT-qPCR Kit Phalaenopsis, Arum Lily 100 RPQ01-TSW TSWV SYBR RT-qPCR Kit Phalaenopsis, Tomato, Prairie Gentian 100 RPQ01-ArM ArMV SYBR RT-qPCR Kit Lily, Tulip 100 RPQ01-TRV TRV SYBR RT-qPCR Kit Lily,Tulip 100 RPQ01-LSV LSV SYBR RT-qPCR Kit Lily,Tulip 100 RPQ01-BYM BYMV SYBR RT-qPCR Kit Gladiolus, Arum Lily, Prairie Gentian 100 RPQ01-LNV LNV SYBR RT-qPCR Kit Arum Lily, Prairie Gentain 100 RPQ01-TuM TuMV SYBR RT-qPCR Kit Arum Lily, Prairie Gentain 100 RPQ01-OR ORSV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-CYM CymMV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-CA CaCV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-OF OFV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-PhCS PhCSV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-CarM CarMV SYBR RT-qPCR Kit for Phalaenopsis Phalaenopsis 100 RPQ01-BA BaMV SYBR RT-qPCR Kit for Bamboo Bamboo 100 RPQ01-PLD PLDMV SYBR RT-qPCR Kit for Papaya Papaya 100 RPQ01-PRS PRSV SYBR RT-qPCR Kit for Papaya Papaya 100 GeneMark 150

GeneMark GMbiolab Co., Ltd. Plant Virus Detection Plant Virus Detection SYBR RT-qPCR Detection Kits Ordering Info Cat.No. Plant Reaction RPQ01-TYL TYLCV SYBR qpcr Kit for Tomato Tomato 100 RPQ01-ToL ToLCV SYBR qpcr Kit for Tomato Tomato 100 RPQ01-BBT BBTV SYBR qpcr Kit for Banana Banana 100 RPQ01-BSV BSV SYBR RT-qPCR Kit for Banana Banana 100 RPQ01-PWV PWV SYBR RT-qPCR Kit for Passion Fruit Passion Fruit 100 RPQ01-DsM DsMV SYBR RT-qPCR Kit for Flamingo Flower Flamingo Flower 100 RPQ01-OrM OrMV SYBR RT-qPCR Kit for Ornithogalum Ornithogalum 100 RPQ01-TRS TRSV SYBR RT-qPCR Kit for Lily Lily 100 RPQ01-LVX LVX SYBR RT-qPCR Kit for Lily Lily 100 RPQ01-LMo LMoV SYBR RT-qPCR Kit for Lily Lily 100 RPQ01-TBV TBV SYBR RT-qPCR Kit for Tulip Tulip 100 RPQ01-DsM DsMV SYBR RT-qPCR Kit for Arum Lily Arum Lily 100 RPQ01-CCS CCSV SYBR RT-qPCR Kit for Arum Lily Arum Lily 100 RPQ01-ZaM ZaMV SYBR RT-qPCR Kit for Arum Lily Arum Lily 100 RPQ01-BBW BBWV SYBR RT-qPCR Kit for Prairie Gentian Prairie Gentian 100 RPQ01-PtM PtMV SYBR RT-qPCR Kit for Dragon Fruit Dragon Fruit 100 RPQ01-ZVX ZVX SYBR RT-qPCR Kit for Dragon Fruit Dragon Fruit 100 RPQ01-CVX CVX SYBR RT-qPCR Kit for Dragon Fruit Dragon Fruit 100 151 GeneMark

Custom Services A. Primer Synthesis.....153 B. Gene Synthesis 154 C. Peptide Synthesis...155 D. Custom Antibody Services....157 E. Plant Virus Detection Services.160 F. Experiment Services...162 GeneMark 152

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Primer Synthesis GeneMark cooperates with BioBasic Inc. to synthesize primers with purity greater than 98%. Using the HAP (High Affinity Purification) column method, the primers synthesized possess purity compatible to HPLC analysis method. The primers also undergo MASS analysis to guarantee the quality. HAP is especially suitable for synthesis of general bases. Nucleotide Abbreviations Features Special modifications: 1. Single modification: modification at 3 or 5 of primer Modification Position Modification Position Phosphorothioate Any Biotin 3' or 5' Phosphorylation 3' or 5' TAMRA or Rox 3' or 5' du or di Any Dig 3' or 5' FAM 3' or 5' Cy3 or Cy5 5 Amine Modification 3' or 5' SH or HS-SH 3 TET or HEX 3 JOE 3 or 5 2. Double modification: modification at both 3 and 5 end of primer. Modification Method Modification Method 5' FAM - 3' TAMRA HPLC 5' FAM - 3' Dabcyl PAGE+ HPLC 5' TET- 3' TAMRA HPLC 5' FAM - 3' BHQ-1 PAGE+ HPLC 5' HEX - 3' TAMRA HPLC 5' FAM - 3' ECLIPSE PAGE+ HPLC 5' HEX - 3' Dabcyl PAGE+ HPLC 5' ROX - 3' Dabcyl PAGE+ HPLC Purification Method HAP PAGE HPLC Features < 60 mer primers, suitable for PCR or sequencing experiments. Purity > 98% > 60 mer primers, suitable for PCR or sequencing experiments. Purity > 99% Suitable for PCR, sequencing experiments or use as probes. Purity > 99% 153 GeneMark

Custom Services Custom Services Features Certification: ISO9001:2000 Fast Product Delivery: Synthesized gene fragments of 150 bp~1.5 kb can be delivered in 28 days. Experienced: More than 10 years of gene synthesis experiences. Products supplied all over the world including the United States and many European countries. Professional Manufacturing: BioBasic Inc. department of gene synthesis consists 60 experienced employers to provide high quality and time saving services. Gene Synthesis GeneMark cooperates with BioBasic Inc. department of gene synthesis, providing high quality gene synthesis service. BioBasic Inc. is approved for ISO9001:2000, and guarantees a 100% correct requested sequences. The benefits for artificial gene synthesis is that researchers can obtain genes suitable for expression in prokaryotes, and to carry out point mutations for genetic modification experiments. Gene fragments of 150 bp~1.5 kb cloned into puc or pbluescript vector can be delivered in 28 days. Applications To generate gene variants, SNPs, cdna and predicted genes, and other suitable applications. Specifications for Custom Gene Synthesis > 1 μg lyophilized DNA cloned into a standard vector (puc or pbluescript). Documentations: Sequencing chromatogram trace file Plasmid map DNA sequencing Restriction digestion analysis Summary report Reasonable Price: Manufactured abroad, domestic charges. Gene length Time required for ready delivery (working days) 0.15~1.5 kb 21~28 days 1.5~2 kb 28~36 days 2~3 kb 36~48 days 3~5 kb 70~90 days GeneMark 154

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Peptide Synthesis GeneMark peptide synthesis service cooperates with manufacturers from abroad with peptide synthesis experience of more than 13 years. We guarantee absolute confidentiality and confidential agreement can be signed if insisted. GeneMark provides peptide synthesis of different length and degree of purity ranging from >70%~>98% depending on peptide applications. Applications Recommended Purity Immunograde Peptide Peptide purity >70% Biochemistry Grade Peptide Peptide purity >85% Applications ELISA tests Peptide array Epitope mapping Semi-quantitative enzyme-substrate studies Phosphorylation studies Peptide blocking studies by Western blotting In-vitro bioassays Cell attachment studies High Purity Grade Peptide Peptide purity >95% Quantitative receptor-ligand interaction studies Quantitative blocking and competitive inhibition assays Quantitative phosphorylation studies Quantitative proteolysis studies NMR studies In-vitro bioassays In-vivo studies Generating standard curves Industrial Grade Peptide Peptide purity >98% cgmp peptides for drug studies Cosmetic peptides for cosmeceuticals industry Clinical trials SAR studies 155 GeneMark

Custom Services Custom Services Peptide Synthesis Features Peptide Size and length: 1 mg~1 kg; Peptide length from 2 ~149 a.a. Fast: Time of delivery is usually 1~4 weeks depending on peptide length, complexity (linear or circular, modification) and purity. We also offer special modifications on peptides such as biotin modifications and phosphorylated residues. Item Modifications N,C-terminal Modification Acetylation (N-), HYNIC (N-), DTPA (N-), Formylation (N-), Fatty acid (N-), Myristic acid (N-), Palmytolyl (N-), Benzyloxycarbonylation (N-), CBZ (N-), Amidation (C-), Succinylation (N-) Special Amino acid D-Arg, D-Cys, D-Asp, D-Asn, D-Glu, D-Gln, D-Ser, D-His, D-Thr, D-Trp, D- Leu, D-Ile, D-Met, D-Pro, D-Val,D-Phe, pglu, Hyp, D-Lys,D-Tyr, D-Orn, Orn, Abu, Aib, (D)1-Nal, (D)2-Pal, (D)4-Cl-Phe, Nva, Nle, Hse, Hcy, Pen, Mpa, N-Methyl amino acid (Ala, Phe, Leu, Ile, Val, Gly, Met),Other amino acid, Dinitrobenzoylation (Lys), Lys(Me2), Phosphorylation (Tyr, Ser, Thr, single site), Tyr(SO 3 H 2 ), Ser(octanoic acid) Fluorescence/Dye labeling Biotin (N-Terminal, Y/N Ahx), Biotin (Lys in sequence), Biotin (without Lys in sequence), FITC/5-FAM (N-Terminal, Y/N Ahx), Dansyl (N- Terminal, Y/N Ahx), MCA (N-Terminal), p-nitroanilide (pna, C- Terminal), AMC (C-Terminal) Cyclic Peptide Quenched Fluorescent Peptide Multiple Antigenic Peptide System Protein Conjugation Disulfide bridge 1 st, Disulfide bridge 2 nd, Disulfide bridge 3 rd, Amide cyclic (Side chain, end) Abz/ Tyr (3-NO 2 ), EDANS/DABCYL Asymmetric 4 branches Asymmetric 8 branches KLH BSA GeneMark 156

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Monoclone Antibody Custom Antibody Services GeneMark custom antibody service cooperates with manufacturers from abroad with peptide generation experiences of more than 13 years. We guarantee absolute confidentiality and confidential agreement can be signed if insisted. We can take over the generation at any stage of development with different charges. Product Delivered 0.5~1 ml pre-immune sera 60~80 ml antisera or 2~5 mg purified antibody Test report (ELISA data, Western Blot etc) 2~3 mg peptides (unmodified & phospho) (*Peptide synthesis only) 2~5 DNA clones and Recombinant vector (* Gene expression only) 2~5 clones of hybridoma cell and isotype determination result (*Monoclone only) Project report Cat. no. Item Service Product Delivered Time GSM001 Monoclonal package 1 (From protein to ascites purification) Protein checked by SDS-PAGE immunization and sera collection ELISA identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) 50 μl pre-immune sera (1:10 dilution) 2~5 hybridomas 2~5 mg protein A/G purified antibodies (2 clones) ELISA result Isotype determination result Project report 18~24 wks GSM002 Monoclonal package 2 (From peptide design to ascites purification) Peptide design and synthesis (12~22 aa,85%) Peptide conjugation Immunization and sera collection ELISA identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) 2~3 mg peptides 50 μl pre-immune sera (1:10 dilution) 2~5 hybridomas 2~5 mg purified antibodies (2 clones) ELISA result (>1:80000) Isotype determination result Project report 20~26 wks GSM003 Monoclonal package 3 (From plasmid to ascites purification) Protein expression and purification Immunization and sera collection ELISA identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) 1 mg protein 50 μl pre-immune sera (1:10 dilution) 2~5 clones 2~5 mg purified antibodies (2 clones) ELISA result (>1:80000) Isotype determination result Project report 20~26 wks GSM004 Monoclonal package 4 (From cdna to ascites purification) Gene amplification; vector construction Protein expression and purification Immunization and sera collection ELISA identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) Recombinant vector 1 mg protein 50 μl pre-immune sera (1:10 dilution) 2~5 clones 2~5 mg purified antibodies (2 clones) ELISA result (>1:80000) Isotype determination result Project report 22~26 wks 157 GeneMark

Custom Services Custom Services Custom Antibody Services Monoclone Antibody Cat. no. Item Service Product Delivered Time GSM005 Monoclonal package 5 (Phospho-specific monoclonal antibodies) 1 peptide design and synthesis (12~22 aa, 85%) (unmodified & phospho) Peptide conjugation Immunization and sera collection ELISA identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) 2~3 mg peptides (unmodified & phospho) 50 μl pre-immune sera (1:10 dilution) 2~5 clones; 2~5mg purified antibodies (2 clones) ELISA result (>1:80000) Isotype determination result project report 21~26 wks GSM006 Monoclonal package 6 (Western guaranteed monoclonal antibodies) 2~3 peptide design and synthesis (12~22 aa, 85%) Peptide conjugation Immunization and sera collection ELISA & Western Blot identification Fusion and hybridoma development Isotype determination Subcloning and cell cryopreservation Ascites production and purification (2 clones) 2~3 mg peptides 50 μl pre-immune sera (1:10 dilution) 2~5 clones 2~5 mg purified antibodies (2 clones) ELISA & Western Blot results: At least 1 peptide s antibody guaranteed (ELISA>1:80000; WB>1:500) Isotype determination result Project report 20~26 wks Polyclone Antibody Cat. no. Item Service Product Delivered Time GSP001 Polyclonal package 1 (From protein to antisera) Protein checked by SDS-PAGE Immunization and sera collection ELISA identification 0.5~1 ml pre-immune sera 60~80 ml antisera ELISA result (>1:12000) Project report 10~12 wks GSP002 Polyclonal package 2 (From protein to antigen purified antibodies) Protein checked by SDS-PAGE Immunization and sera collection ELISA identification Antigen affinity purification 0.5~1 ml pre-immune sera 2~5 mg purified antibodies ELISA result (>1:32000) Project report 10~12 wks GSP003 Polyclonal package 3 (From peptide design to antisera) Peptide design and synthesis (12-22aa, 85%) Peptide conjugation Immunization and sera collection ELISA identification 2~3 mg peptides 0.5~1 ml pre-immune sera/ rabbit 60~80 ml antisera ELISA result (>1:12000) Project report 12~15 wks GSP004 Polyclonal package 4 (From peptide design to antigen purified antibodies) 1 peptide design and synthesis(12-22aa, 85%) Peptide conjugation Immunization and sera collection ELISA identification Antigen affinity purification 2~3 mg peptides 0.5~1 ml pre-immune sera 2~5 mg purified antibodies ELISA result (>1:32000) Project report 12~15 wks GeneMark 158

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Custom Antibody Services Polyclone Antibody Cat. no. Item Service Product Delivered Time 1 mg protein GSP005 Polyclonal package 5 (From plasmid to antisera) Protein expression and purification Immunization and sera collection ELISA identification 0.5~1 ml pre-immune sera/ rabbit 60~80 ml antisera ELISA result (>1:12000) Project report 12~15 wks GSP006 Polyclonal package 6 (From plasmid to antigen purified antibodies) Protein expression and purification Immunization and sera collection ELISA identification Antigen affinity purification 1 mg protein (purity>90%) 0.5~1 ml pre-immune sera/rabbit 2~5 mg purified antibodies ELISA result (>1:32000) Project report 12~15 wks GSP007 Polyclonal package 7 (From cdna to antisera) Gene amplification Vector construction Protein expression and purification Immunization and sera collection ELISA identification Recombinant vector 1 mg protein (purity>90%) 0.5~1 ml pre-immune sera/rabbit 60~80 ml antisera ELISA result (>1:12000) Project report 14~16 wks GSP008 Polyclonal package 8 (From cdna to antigen purified antibodies) gene amplification Vector construction Protein expression and purification Immunization and sera collection ELISA identification Antigen affinity purification Recombinant vector 1 mg protein (purity>90%) 0.5~1 ml pre-immune sera/rabbit 2~5 mg purified antibodies ELISA result (>1:32000) Project report 14~16 wks GSP009 Polyclonal package 9 (Phospho- specific polyclonal antibodies) 1 peptide design and synthesis (12~22 aa,85%) 1 Phospho-specific site Peptide conjugation Immunization and sera collection ELISA identification Antigen affinity purification 2~3 mg peptides (unmodified & phospho) 0.5~1 ml pre-immune sera/rabbit 2~5 mg phospho-specific antibodies 3~5 mg non-phospho-specfic antibodies ELISA result (>1:32000, phosphoantibody) Project report 13~16 wks GSP010 Polyclonal package 10 (Western guaranteed polyclonal antibodies) 2 peptide design and synthesis (12~22 aa,85%) Peptide conjugation Immunization and sera collection ELISA & Western Blot identification Antigen affinity purification 2~3 mg peptides 0.5~1 ml pre-immune sera/rabbit 2~5 mg purified antibodies ELISA & Western Blot results (ELISA > 1:32000, WB > 1:200) At least 1 peptide s antibody guaranteed Project report 15~18 wks GSP011 Polyclonal package 11 (Western and IHC guaranteed polyclonal antibodies) 3 peptide design and synthesis (12~22 aa,85%) Peptide conjugation Immunization and sera collection ELISA identification Western Blot & IHC identification Antigen affinity purification 2~3 mg peptides 0.5~1 ml pre-immune sera/rabbit 2~5 mg purified antibodies ELISA result, Western Blot & IHC results (ELISA>1:32000; WB>1:200) At least 1 peptide s antibody guaranteed Project report 15~18 wks 159 GeneMark

Custom Services Custom Services Plant Virus Detection Services GeneMark professional bio lab provides fast and accurate plant virus detection services. Different detection methods including ELISA detection, RT- PCR detection and real-time PCR detection are available depending on customers needs. Ordering Info DESCRIPTION ELISA detection service of CMV-group 1 virus ELISA detection service of Phalaenopsis (CymMV) ELISA detection service of Phalaenopsis (ORSV) ELISA detection service of Phalaenopsis (CaCV) ELISA detection service of Phalaenopsis (INSV) ELISA detection service of Phalaenopsis (TSWV) ELISA detection service of Papaya (PRSV) ELISA detection service of Papaya (PLDMV) RT-PCR detection of Phalaenopsis (C+O) RT-PCR detection of Phalaenopsis (CaCV) RT-PCR detection of Phalaenopsis (CaCV+CMV) RT-PCR detection of Phalaenopsis (TSWV+INSV) RT-PCR detection of Bamboo (BaMV) RT-PCR & NEST PCR detection of Bamboo (BaMV) RT-PCR detection of Papaya (PRSV+PLDMV) RT-PCR detection of Melon (WSMoV) RT-PCR detection of Melon (MYSV) RT-PCR detection of Melon (CCYV) RT-PCR detection of Dragon Fruit (PtMV) RT-PCR detection of Dragon Fruit (ZVX) RT-PCR detection of Dragon Fruit (CVX) Cat.No. GMS-E04CM GMS-E01CM GMS-E02OR GMS-E03CA GMS-E05IN GMS-E06TS GMS-E08PRS GMS-E09PLD GMS-R01CO GMS-R01CA GMS-R01CC GMS-R01TI GMS-R01BA GMS-R01NBA GMS-R01PP GMS-R01WSMo GMS-R01MYS GMS-R01CCY GMS-R01PtM GMS-R01ZVX GMS-R01CVX GeneMark 160

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Plant Virus Detection Services Ordering Info DESCRIPTION RT-PCR detection of Tomato (TYLCV) RT-PCR detection of Tomato (ToLCV) RT-qPCR detection of Phalaenopsis (C+O) RT-qPCR detection of Phalaenopsis (C+O+CaCV) RT-qPCR detection of Phalaenopsis (C+O+CaCV+CMV) RT-qPCR detection of Phalaenopsis (CaCV+CMV) RT-qPCR detection of Phalaenopsis (TSWV+INSV) RT-qPCR detection of Bamboo (BaMV) RT-qPCR detection of Papaya (PRSV+PLDMV) Cat.No. GMS-R02TYL GMS-R02ToL GMS-RQ01CO GMS-RQ01COA GMS-RQ01COAC GMS-RQQ01CC GMS-RQ01TI GMS-RQ01BA GMS-RQ01PP 161 GeneMark

Custom Services Custom Services Experiment Services GMbiolab offers a wide range of professional experiment services and delivers accurate and high-quality results. Our services include: Plasmid DNA Purification Genomic DNA, Total RNA Extraction PCR/RT-PCR/real-time PCR TA cloning Custom Competent Cells Plasmid DNA Purification Freshly prepared plasmid ( > 1 ng) or bacteria culture ( > 2 x 10 9 ) is required for high-quality plasmid purification results. We also offer the removal of endotoxin or endonucleases depending on your requests. (Scale: 1 mg, 5 mg, 10 mg and > 10 mg) Genomic DNA, Total RNA Extraction We recommend storage of tissue or centrifuged pellet in GeneMark RNA SafeGuard solution to prevent RNA degradation. Minimum sample required: Bacteria ( > 2 10 9 ); Cell ( > 5 10 6 ); Animal tissue ( > 25 mg); Plant ( >100 mg). PCR/RT-PCR/real-time PCR GMbiolab provides Oligo dt and Random hexamers as primers. Customers may have to provide primers for specific amplification. (5 μl of 10 μm concentrated primer per reaction) Minimum sample required: RNA (>100 ng); gdna(>3 ng); Plasmid (>1 ng); cdna (>100 ng). Sample is not limited to the above recommendation volume if specific concentration is assigned. GeneMark 162

GeneMark GMbiolab Co., Ltd. Custom Services Custom Services Experiment Services TA Cloning GMbiolab offers TA cloning of up to 6 kb PCR product into TA vector. Gel slice is accepted. Minimum sample required: PCR product > 5 μg Custom Competent Cells Fresh bacteria strain must be provided by customer for GMbiolab to prepare competent cells. Transformation efficiency varies depending on bacteria strains, however we do guarantee 10 5 ~10 8 cfu/μg of transformation efficiency. Our professionals will evaluate the bacterial strain and will start the preparation only after the customer has accepted the transformation efficiency. 163 GeneMark

GMbiolab Co., Ltd. (GeneMark) Phone: +886-4-2407-3270 Fax: +886-4-2406-1513 E-mail: info@genemarkbio.com www.genemarkbio.com