8, 27 2009 MARINE WATERS SURROUNDING SWEDEN



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Oceanographic Unit No 8, 27 July-1 Aug 2009 ALGAL SITUATION IN MARINE WATERS SURROUNDING SWEDEN Abstract The phytoplankton diversity was overall low in the Skagerrak area. The diversity was although higher at the more inshore station Släggö where several species were found. Dominating species at this site were both the diatom Pseudo-nitzschia and the dinoflagellate Prorocentrum micans. The water column was well mixed and only Å17 had a chlorophyll fluorescence maximum at 30-40 meters with a dominance of the dinoflagellate Ceratium longipes. The diversity was higher in the Kattegat except for Fladen. The diatom Proboscia alata dominated the integrated samples at all stations. A chlorophyll fluorescence maximum was recorded at Anholt E at 30-40 meters and at Landskrona at 15 meters. The dominating species at the maximum was the dinoflagellate Ceratium longipes at Anholt E and the diatom Guinardia flaccida at Landsrona. In the Baltic, small surface accumulations of cyanobacteria were observed east of BY5 and observed on and of until BCS III-10. No accumulations were observed north of BCS III-10 but both N. spumigena, Aphanizomenon spp. and Anabaena spp. were detected in both surface samples and in integrated samples. The winds stress probably disolved the patchess. The amount of N. spumigena* and Aphanizomenon spp decreased going Southwest of Gotland and only small amounts were found in the Kalmar sound. Anabaena spp. seemed however to increase slightly in the southern part of Kalmar sound. To follow the surface accumulations of cyanobacteria in the Baltic Sea by satellite interpretations and high resolution images: http://www.smhi.se/cmp/jsp/polopoly.jsp?d=7826&l=en The phytoplankton samples were filtered through 10 µm polycarbonate filters before being analysed using a light microscope. Potentially toxic species are marked with *. To observe which of the cyanobacteria species were dominating the surface accumulations, bucket sampling was performed at most of the Baltic stations. Small species, e.g. Chrysochromulina polylepis* can not be analysed onboard and will be exluded from this reduced AlgAware report

59 o N 58 o N Å17 Släggö BY15 57 o N Anholt E BY38 56 o N 55 o N BY2 BY5 BCS III-10 54 o N 9 o E 12 o E 15 o E 18 o E 21 o E The Skagerrak Å17 27 th of July (open Skagerrak) The phytoplankton diversity was low, the dinoflagellate genus Ceratium was observed. A chlorophyll fluorescence maximum at 30-40 meters depth was dominated by Ceratium longipes. Släggö 27 th of July (Skagerrak coast) A quite diverse community was found. The dinoflagellate Prorocentrum micans dominated together with the diatom genus Pseudo-nitzschia. The dinoflagellate Dinophysis acuminata* was also observed P2 29 th of June (Skagerrak coast) The diatom Proboscia alata dominated the community. Several species of the dinoflagellate genus Ceratium was found together with a few individuals of D. acuminata * and D. norvegica * The Kattegat Fladen 30 th of June Few species were found. The diatom Proboscia alata dominated the sample. Several species of the dinoflagellate genus Ceratium was also found.

N14 Falkenberg 30 th of June The diatom, Proboscia alata, dominated hear as well but in a slightly more diverse community. A few filaments of the cyanobacteria Nodularia spumigena* were found. The dinoflagellate Dinophysis norvegica* was also found. Anholt E 30 th of June The diatom Proboscia alata dominated the sample once again. The dinoflagellate Ceratium tripos was also quite common. A chlorophyll fluorescence maximum was located at 25-30 meters where the dinoflagellate genus Ceratium dominated and far most Ceratium longipes. The dinoflagellates Dinophysis acuminata* and D. norvegica* was also. The community was more or less the same on the second stop at the station but no chlorophyll fluorescence maximum was this time. W Landskrona 30 th of June A divers community was found. The diatom Proboscia alata although continued to dominate. Several species of dinoflagellates were found for example Dinophysis acuminata *, D. norvegica * and Protoceratium reticulatum *. Several species of the genus Chaetoceros together with the genus Pseudo-nitzschia spp.* was noted among the several diatoms found. Both the filamentous cyanobacterium Nodularia spumigena* and the genus Anabaena spp. were found in low abundances. Selection of observed species Å17 Släggö N14 Anholt E Anholt E Red=potentially toxic species 2009-07-27 2009-07-27 2009-07-28 2009-07-28 2009-08-01 1 quantified in m/l cells/l cells/l cells/l cells/l cells/l Chaetoceros curvicetus Chaetoceros socialis Chaetoceros spp. Guinardia flaccida Proboscia alata common common common Pseudo-nitzschia spp. common Thalassiosira spp. Ceratium furca Ceratium fusus Ceratium lineatum Ceratium longipes Ceratium macroceros Ceratium tripos common Dinophysis acuminata Dinophysis norvegica common Dinophysis rotundata Lingulodinium polyedrum Prorocentrum micans common Protoceratium reticulatum Protoperidinium depressum Protoperidinium oblongum Protoperidinium steinii Protoperidinium spp. Scrippsiella complex Dictyoca fibula Anabaena spp. 1 0.01 Nodularia spumigena 1 0.02

The Baltic Sea The first signs of surface accumulations of cyanobacterium were observed east of BY5. The accumulations looked like small grains. A surface sample was taken underway and analysis made clear that the filamentous cyanobacteria N. spumigena* were. The accumulations persisted until BCS III-10. No clear accumulation was found north of BCS III-10 but surface samples and integrated samples contained relatively high abundance of N. spumigena* and Aphanizomenon spp at all stations untill BY20 northeast of Gotland. No accumulations were found west of Gotland or in the Kalmar sound. The occurrence of both N. spumigena and Aphanizomenon spp decreased going south and only a few filaments could be found at M1-V1. Anabaena spp. seemed however to increase going south and a small peak was observed at M1-V1. Only a few filaments of N. spumigena* were noted in surface samples at Hanö bight. Arkona Basin BY2 and Bornholm Basin and BY5 29 th of July Aphanizomenon spp. was the most common of the cyanobacterium but Nodularia spumigena* was also. The diatom Chaetoceros impressus was very common at both BY2 and BY5. South East Baltic BCS III-10 29 th of July Few species wer found in the integrated sample. The diatom C. impressus dominated. A chlorophyll fluorescence maximum was located at 15 meters containing the same species as the integrated sample except that a few filaments of the cyanobacteria genus Aphanizomenon were found. Both the cyanobacterium Anabaena spp. and N. spumigena* were in the integrated sample in low amounts and N. spumigena* dominated at the surface. Eastern Gotland Basin BY15 30 th of July The diatom Chaetoceros impressus dominated the sample. Several filaments of the cyanobacterium N. spumigena* Aphanizomenon spp. and Anabaena spp. were both in the surface and the integrated sample. The dinoflagellate D. norvegica* as well as large filaments of Aphanizomenon spp. were found at the chlorophyll fluorescence maximum at 15 meters. Western Gotland Basin BY 38 31 th of July Aphanizomenon spp., N. spumigena* and Anabaena spp. were found in small amounts where N. spumigena dominated. A few individuals of the genus Dinophysis* was found and a few other cells in a rather scars sample. Kalmar Sound Ref. M1-V1 31 th of July The sample was thin and contained few species. The dinoflagellate Heterocapsa triquetra dominated. Anabaena spp. dominated the filamentous cyanobacterium but a

few filaments of Aphanizomenon spp. and N. spumigena* were also found. Both Dinophysis norvegica* and D. acuminata* was. Phytoplankton analysis and text by: Marie Johansen Selection of observed BY2 BY5 BCS III-10 BY15 BY38 Ref. M1-V1 species Red=potentially toxic 2009-07-29 2009-07-29 2009-07-29 2009-07-30 2009-07-31 2009-07-31 species 1 quantified in m/l cells/l cells/l cells/l cells/l cells/l cells/l Chaetoceros impressus 3 300 4 000 3 700 Chaetoceros similis Chaetoceros spp. Thlalassiosira spp. Ceratium tripos Dinophysis acuminata Dinophysis norvegica Dinophysis rotundata Gonyaulax spp. Gymnodiniales Heterocapsa triquetra common Anabaena spp. 1 Aphanizomenon spp. 1 0.8 Nodularia spumigena 1 1,2 1,2 Surface sampling using a bucket. Observations of the following filamentous cyanobacteria: Station: Aphanizomenonspp. Nodularia spumigena* Anabaena spp. BY2 BY4 BY5 East of BY5 BCS III-10 BY10 common BY15 BY20 common BY32 BY38 Hanö bight Ref M1 V1 common

Art / Species Gift / Toxin Eventuella symptom Clinical symptoms Alexandrium spp. Paralytic shellfish poisoning (PSP) Dinophysis spp. Pseudochattonella spp. Chaetoceros concavicornis Pseudo- niztschia spp. Diarrehetic shellfish poisoning (DSP) Fish toxin Mechanical damage through hooks on setae Amnesic shellfish poisoning (ASP) Milda symptom: Inom 30 min.: Stickningar eller en känsla av bedövning runt läpparna, som sprids gradvis till ansiktet och nacken; stickningar i fingertoppar och tår; Huvudvärk; yrsel, illamående, kräkningar, diarré Extrema symptom: Muskelförlamning; andningssvårigheter; känsla av att kvävas; Man kan vara död inom 2-24 timmar efter att ha fått i sig giftet, på grund av att andningsmuskulaturen förlamas. Milda symptom: Efter cirka 30 minuter till några timmar: yrsel, illamående, kräkningar, diarré, magont Extrema symptom: Upprepad exponering kan orsaka cancer Låg celltäthet: Ingen påverkan. Hög celltäthet: Fiskens gälar skadas, fisken dör. Låg celltäthet: Ingen påverkan. Hög celltäthet: Fiskens gälar skadas, fisken dör. Milda symptom: Efter 3-5 timmar: yrsel, illamående, kräkningar, diarré, magkramper Extrema symptom: Yrsel, hallucinationationer, förvirring, förlust av korttidsminnet, kramper Mild case: Within 30 min: tingling sensation ro numbness around lips, gradually spreading to face and neck; prickly sensation in fingertips and toes; headake, dizziness, nausea, vomiting, diarrhoea. Extreme case Muscular paralysis; pronounced respiratory difficulty; choking sensation; death trough respiratory paralysis may occur within 2-24 hours after ingestion. Mild case: Within 30 min-a few hours: dizziness, nausea, vomiting, diarrhoea, abdominal pain. Extreme case: Repeated exposure may cause cancer. Low cell numbers: No effect on fish. High cell numbers: Fish death due to gill damage. Low cell numbers: No effect on fish. High cell numbers: Fish death due to gill damage. Mild case: Within 3-5 hours: dizziness, nausea, vomiting, diarrhoea, abdominal cramps. Extreme case: dizziness, hallucinations, confusion, loss of memory, cramps.