Basics of Wild Harvested Mushroom Identification

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Basics of Wild Harvested Mushroom Identification Dennis E. Desjardin Professor of Mycology Pacific Region Retail Food Seminar 9-11 Sept. 2014 Phoenix, AZ

Status of Wild Harvested Commercial Mushrooms Pre-1980: Mostly recreational collecting of wild mushrooms with small quantities sold to restaurants; few commercial collectors; few established buyers; collecting permits mostly not required Post-1980: Many commercial collectors and established buyers; collecting permits required; developed globally into a multi-million dollar industry; >$80,000,000 per year for western US Current Status: No universal collecting regulations Permits to commercially collect wild mushrooms vary by city, county, state, district or park No regulations for selling whole, unprocessed, wild harvested mushrooms [Processed mushrooms require special permits, facilities and labeling] No oversight concerning accurate identification or quality No labeling concerning proper preparation before ingestion

Guidelines for a Model Wild Harvested Mushroom Program Submitted by Chris Gordon & Lisa Roy Accepted by Assembly of Delegates on April 16, 2012 Updated May 5, 2014 Develop resources & criteria to select wild mushroom species for service or sale Establish record-keeping and traceability to assure safety of wild harvested mushrooms Develop a wild harvested mushroom curriculum to train approved mushroom identifiers Create an exam so that approved mushroom identifiers can demonstrate competence identifying mushrooms State and local jurisdictions should develop and implement their own programs and have flexibility in establishing the approval process

Wild Harvested Commercial Mushrooms in the Pacific Region (excluding the Pacific Islands) Top 7 Commercial Species: golden chanterelles (various Cantharellus species) morels (various Morchella species) American matsutake (Tricholoma magnivelare) king bolete; porcini (B. edulis) black chanterelles (Craterellus cornucopioides, C. fallax) lobster mushroom (Hypomyces lactifluorum) candy caps (Lactarius rubidus, L. rufulus) Additional 37 Commercial Species: queen bolete (B. regineus) spring king bolete (B. rex-veris) southwest mountain porcini (Boletus rubriceps) butter boletes (various Butyriboletus species) white chanterelle (Cantharellus subalbidus) winter chanterelle; yellow foot (Craterellus tubaeformis) blue chanterelle (Polyozellus multiplex) pig's ears (Gomphus clavatus) lion's mane (Hericium erinaceus) hedgehogs (Hydnum repandum; H. umbilicatum) shingled hedgehog (Sarcodon imbricatus) shrimp mushroom (Russula xerampelina) coccoli, coccora (Amanita calyptroderma) sun caesar (Amanita basii) oyster mushroom (Pleurotus ostreatus) man on horseback (Tricholoma equestre) poplar tricholoma (Tricholoma populinum) streaked tricholoma (Tricholoma portentosum) delicious or bleeding milk caps (Lactarius deliciosus and allies) fried chicken mushroom (Lyophyllum decastes) coral mushrooms (various Ramaria species) club corals (Clavariadelphus truncatus, C. occidentalis) greening goat's foot (Albatrellus ellisii) false morels; brain mushrooms (Gyromitra esculenta, G. montana) early morel (Verpa bohemica) bell morel; thimble fungus (Verpa conica) Oregon white truffles (Tuber oregonense, T. gibbosum) Oregon black truffle (Leucangium carthusianum) Oregon brown truffle (Kalapuya brunnea) puffballs (various Calvatia s.l. species) cauliflower mushroom (Sparassis radicata) beefsteak mushroom (Fistulina hepatica) sulphur shelf (Laetiporus conifericola, L. gilbertsonii) hen of the woods (Bondarzewia mesenterica) shaggy parasol (Chlorophyllum brunneum, C. rachodes) shaggy mane (Coprinus comatus) honey mushrooms (various Armillaria species) Data generated from personal observations and interviews with buyers and sellers

Mushroom Identification Mushroom identification is based on a combination of macromorphological, micromorphological and physiological characters, ecology and DNA sequences (multiple genes). For identifying wild harvested commercial mushrooms, only the use of macromorphological characters is practical. Characters differ depending of species group, but most wild harvested commercial mushrooms can be distinguished from look-alike species by macro-features of their body form, cap, spore-bearing surface, stipe, veils and odor. Important features include shape, size, color, topology, ornamentation and texture.

Step 1: Identify body form * Hymenophore = structure on which the spores are formed Gilled Fungi Chanterelles Hymenophore of radially arranged, thin, sharp-edged plates Hymenophore of radially arranged, thick, rounded-edged ridges * Only body forms of wild harvested commercial mushrooms are addressed here

Boletes Polypores Hymenophore of vertically oriented tubes, easily separated from cap Fruitbodies putrescent, terrestrial Stipe present Hymenophore of vertically oriented tubes, inseparable from cap Fruitbodies persistent, lignicolous Stipe typically absent

Spine Fungi Coral Fungi Hymenophore of vertically oriented spines; spores produced on surface of spines Hymenophore of multiple branches; spores produced on surface of branches

Puffballs Morels Hymenophore internal, powdery at maturity; spores released when cap ruptures Hymenophore of fertile pits and sterile ridges

Hypogeous Fungi Truffles Jelly Fungi Hymenophore internal, solid at maturity; fruitbodies underground; strong odors Hymenophore on external surface; texture gelatinous

Gilled Mushroom and Bolete Body Forms Cap = Pileus Universal Veil Hymenophore Gills or Tubes Partial Veil Stem = Stipe Gilled Mushroom Bolete

Step 2: Determine cap features Size width at maturity Color when young and old Shape when young and old convex conical umbonate depressed or funnel-shaped umbilicate

Topology on disc and margin smooth rugulose areolate striate surface even wrinkled or pitted cracked radially grooved Ornamentation type, size and arrangement of surface hairs glabrous fibrillose squamulose velutinous lacking ornamentation with radial hairs scaly velvet-like

Tactile Response when young and old dry viscid glutinous sticky slimy Universal Veil Structure a layer of tissue that encloses entire mushroom when young staining on the surface or in the context many colors scales or flakes membranous patch fibrillose

Step 3: Determine hymenophore features Gilled Fungi Gill Color when young and old; often represents spore color Gill Spacing distance between gills Gill Attachment to Stipe free adnexed adnate decurrent not attached to stipe curved upward attached at 90 curved downward Spore Color determined from a spore print on paper or deposit on stipe apex black pinkish brown

Tube Color when young and old Boletes white yellow red blue stained Tube and Pore Shape small, round pores large, angular pores stuffed pores diagnostic for the porcini group

Step 4: Determine stipe features Size length & width at maturity Color at apex and base Shape cylindrical clavate bulbous rooting Ornamentation type, size and arrangement of surface hairs velutinous reticulate glandular scabrous

Partial Veil a tissue that encloses the hymenophore when young, then ruptures and remains on the cap margin or stipe apex = annulus Immature partial veil covering hymenophore membranous fibrillose = cortina fibril zone glutinous appendiculate

Step 5: Additional taxonomic features Odor and Taste break the cap and smell the context Taste a small portion of the cap and hymenophore garlic, ground meal, cucumber, green corn, radish, apricot, citrus, geranium, cardamom, almond, spicy, rancid, skunk, cedar chest, leather, burned rubber, smoke, phenol, ammonia or mushroomy These two species grow in grasslands, both stain yellow and are nearly identical Agaricus fissuratus, an excellent edible with almond odor Agaricus xanthodermus, a problematic toxic species with phenol odor Substrate if possible, determine where the species grows and on what soil, leaves, wood, woodchips, dung

Identifying Wild Harvested Commercial Mushrooms Chanterelles Cantharellus californicus In the Pacific Region, all four of these species are sold as Chanterelles. All are edible, easy to identify as chanterelles, but difficult to identify to species. For commercial purposes, identification to species rank is not important. But there are some look-alikes that are not chanterelles! Cantharellus roseocanus Cantharellus subalbidus Cantharellus formosus

Chanterelles & look-alikes Golden chanterelle False chanterelle look-alikes Cantharellus californicus Hymenophore of radial ridges and folds with rounded edges; deeply decurrent Odor slightly fruity Terrestrial Cuphophyllus pratensis Hymenophore of sharp-edged gills; not forked; shallowly decurrent Odor indistinct Terrestrial Hygrophoropsis aurantiaca Hymenophore of sharpedged gills; conspicuously forked; shallowly decurrent Odor indistinct Lignicolous

American matsutake American matsutake Matsutake look-alikes Tricholoma magnivelare Cap white; rusty stains Gills adnate Odor strongly spicy Partial veil single, thin, cottony Catathelasma ventricosum Cap with grey tones Gills decurrent Odor of ground meal Partial veil double, thick, membranous Tricholoma vernaticum Cap with grey tones Gills adnate Odor of cucumber Partial veil poorly developed, fibrillose

Morels Morels False Morels False morels often cause gastrointestinal distress and are sometimes sold as morels, especially when dried Morchella rufobrunnea Gyromitra esculenta Morchella sextelata Cap cone-shaped, with fertile pits and sterile ridges Gyromitra montana Cap brain-shaped, with convoluted folds, fertile overall

Prized edibles Porcini Boletus edulis Edible Boletes Although these are all edible species, Suillus are often misrepresented as porcini (Boletus edulis group), especially when sold dried. Poor substitutes Slippery Jacks Suillus brevipes Boletus regineus Pores tiny, round, stuffed when young Stem apex reticulate Suillus pungens Pores larger, angular, not stuffed when young Stem apex glandular

Lobster Mushroom This easily identified commercial edible represents two different species: the crustose fungal pathogen Hypomyces lactifluorum growing on a number of different Russula and Lactarius species. Notice the mushroom hymenophore is underdeveloped and sterile. Consumption of the host raw causes gastrointestinal distress; hence the duo must be cooked thoroughly before ingestion. Hypomyces lactifluorum on Russula brevipes

Species that should NOT be sold commercially but currently are: " " " false morels Gyromitra species cause gastrointestinal distress in many people; deadly look-alikes coccora Amanita calyptroderma easy to confuse with the deadly Amanita phalloides coral mushrooms Ramaria species species with orange branches & yellow tips are often toxic early morel Verpa bohemica cause gastrointestinal distress in many people shaggy parasols Chlorophyllum species cause gastrointestinal distress in many people man on horseback Tricholoma equestre prolonged use causes heart and liver disease poplar tricholoma Tricholoma populinum easy to confuse with toxic species streaked trichloma Tricholoma portentosum easy to confuse with toxic species shingled hedgehogs Sarcodon species many are too bitter, cause gastrointestinal distress in many people

False Morels Gyromitra esculenta Gyromitra infula Gyromitra montana When cooked thoroughly, these two species are edible to some people This species can be deadly if ingested raw or cooked. It is easily confused with Gyromitra esculenta

Coccora or Coccoli Coccora is a good edible mushroom, but is easily confused with Amanita phalloides, the Death Cap mushroom, the cause of most human and dog mushroom-related deaths Amanita calyptroderma Cap yellow to orangish brown Cap margin striate Stem hollow; base not bulbous Universal veil thick, leaving a patch on the cap and a thick, saccate volva Amanita phalloides Cap yellowish green Cap not striate Stem solid; base bulbous Universal veil thin, cap patch often absent; saccate volva thin

Coral Mushrooms Many coral mushrooms are edible, but some cause serious gastrointestinal upset. Corals are notoriously difficult to identify. Ramaria formosa Ramaria rubricarnata Ramaria flavigelatinosa All of these coral species have orange branches with yellow tips. Some are edible but others are toxic! Ramaria gelatiniaurantia Ramaria conjunctipes

Warning Labels Required No guidance is provided to the buyer of commercial wild mushrooms as to how they should be prepared before consumption. Some species must be cooked. The following warning should be posted with the species listed below: Mushrooms should be cooked thoroughly. Consumption raw or undercooked may cause gastrointestinal upset. morels bell morel lobster mushroom sulphur shelf

Summary At least 44 wild harvested mushroom species are sold to retailers and restaurants in the Pacific Region. Some species are collected throughout the region, while others are specific to a particular area. The sale of these species is currently unregulated, and there is no oversight concerning accurate identification or quality. Most of the wild harvested commercial species are easily identified by a combination of macromorphological features, and are easily distinguished from look-alike species. However, the identification of some commercial mushroom species is more problematical and confusion with toxic lookalikes is possible. The sale of a few wild harvested commercial species should be halted because they either cause gastrointestinal upset in many consumers, or they are too easily confused with toxic species. Warning indications that some wild harvested commercial species must be thoroughly cooked before ingestion should be provided to buyers and food preparers.

Resource for identifying Wild Harvested Commercial Mushrooms Over 1100 species treated Complete descriptions of 650 species >90% occur throughout western US Each species illustrated with a color photograph Complete keys to aid identification Up-to-date taxonomy & nomenclature 8½ x 11 format, 580 pages Hardcover from Timber Press available 12 Nov. 2014 ISBN-13: 978-1-60469-353-9 www.californiamushrooms.us All photographs in this presentation are from California Mushrooms

Thank You Agaricus campestris Cantharellus formosus