PGR Strategies for Improving Production Practices

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Horticulture Section, Cornell University Geneva, NY PGR Strategies for Improving Production Practices Poliana Francescatto 2016 Eastern NY Commercial Tree Fruit School Feb 17, 2016

PGRs Their Roles and Uses Increasing fruit set (AVG/ReTain cherries and pears; Promalin after frost damage) Fruit thinning (6BA, NAA, NAD, ACC, ABA) Growth control (Apogee, Kudos) Fruit russet control (GA4/7-ProVide) Fruit shape control, fruit finish (GA4/7 + 6BA Promalin, ProGibb reduces blush in Golden and Granny) Fruit color improvement (MeJ-Blush, Stimplex) Fruit maturity and fruit drop control (Retain/NAA/Harvista, ProGibb - cherries)

Precision application of PGRs on Fruit crops - Plant Growth Regulators (PGRs) are an investment - Unlike traditional crop protection products; - Increase value of crop. - Plant response is often variable/inconsistent; - Responses to PGRs are strongly influenced by: - Enviroment, application timing, rate, application volume, coverage, water quality, adjuvants Cultivar, rootstock, tree age, tree vigor, crop load, training system, tree nutrition, aplication equipment

Precision application of PGRs on Fruit crops - Effects of Environment Temperatures before application - CHO demand (cooler temps = less CHO demand = less stress) - Plant physiology process (pollen tube growth, cell division rates) Temperatures during application - Absorption & uptake (warmer temps = greater uptake (>50F but <85F for thinning) - Drying time

Precision application of PGRs on Fruit crops - Effects of Environment Temperatures after application - Higher temperatures following application: Increase response to post-blossom thinners Increase ripening response to Ethephon and NAA Decrease stop-drop response to Retain - Humidity - Increasing humidity increases absorption Slow drying time Increases absorption from deposit

Precision application of PGRs on Fruit crops - Rain - wash-off if too soon after application - re-wetting of deposits (NAA 2 days after application) - Effects of coverage What is your target? Flowers, leaves or fruit? Many PGRs have limited movement - Surfactants (Retain, ProGibb, CPPU, Apogee) - Reduce surface tension, increasing contact area - Do not apply surfactant when the purpose is to increase fruit set.

Summarizing High temperatures, slow drying conditions and healthy foliage will enhance absorption and increase plant response. Cool temperatures, fast drying conditions, and damaged trees or foliage will decrease plant response. If the weather is cool and humid, morning applications are best; If it s been hot and dry, an evening application will be most effective.

Ongoing PGRs Projects in Geneva 1) Validate the accuracy of the Precision Thinning Protocol using the carb and FGR model; 2) Develop improved thinning treatments for existing and new varieties; 3) Testing new compounds for fruit thinning; 4) Develop return bloom treatments to overcome biennial bearing; 5) Investigate a new strategy to control bitter pit; 6) Develop improved pre-harvest drop control strategies;

Ongoing PGRs Projects in Geneva

NY1 SnapDragon, NY2 Rubyfrost, Sweetango (Planted in 2010 - M9/337) Rubyfrost

Fruit Number /tree NY1 SnapDragon, NY2 Rubyfrost, Sweetango (Planted in 2010 - M9/337) 300 250 200 150 100 * Sprayed at 12mm NY1 NY2 Sweetango 50 0 2oz 6oz 32oz 64oz

Untreated Control 15ppm NAA + 1pt Sevin at12mm Rubyfrost

Testing Metamitron (Brevis) for fruit thinning Sugar beet herbicide photosynthesis inhibitor Can cause phytotoxicity in very high rates (leaf damage) Applied at normal thinning window Works best in carbohydrate deficit Registered in EU, to be registered in the US in the near future

Efficacy of metamitron in Geneva 2015 Brevis 15% - Sugar beet herbicide Location: Experimental orchard at Cornell Station in Geneva, NY Variety/age: Crimson Gala/M9 17 years old (1998) Tree density: 558 trees/acre Petal Fall: 05/18/15 and 12mm: 05/21/15 Treatments: Control Metamitron 200 ppm PF Metamitron 300 ppm PF Metamitron 400 ppm PF Metamitron 300 ppm+ 100ppm BA PF Metamitron 200 ppm 12mm Metamitron 300 ppm 12mm Metamitron 400 ppm 12mm Metamitron 300 ppm+ 100ppm BA 12mm BA 100 ppm + 1pt Sevin 12mm

Efficacy of metamitron in Geneva 2015 * Gala

Results from Brazil Untreated trees 100 ppmmetamitron + BA 60 ppm at petall fall

Testing ACC for fruit thinning Precursor of ethylene Works well as pome and stone fruit thinner Applied up to 20 mm fruitlet size (after thinning window - RESCUE THINNER) Not registered, to be registered in 5-6 years from now Works also as apple coloring agent (ethylene effect) to counteract ReTain s color depressing effect

Testing new compounds for fruit thinning Determine the effect of ACC on fruit thinning of Golden Delicious and Cameo. It seems the effect of ACC is cultivar-dependent. Golden no leaf drop occurred Cameo = noticeable, but not damaging, leaf drop at the highest rate of ACC

ACC rescue thinning Spray at 18mm fruit size Golden = no leaf drop occurred Cameo = noticeable leaf drop at the highest rate of ACC.

64oz Maxcel +Sevin at 10mm + 150ppm ACC + Silwet at 18mm

ABA ABA is a mild apple/pear thinner Good combination partner for other chemicals (6-BA) Works best in carbohydrate deficit Applied at 150-400 ppm Registered as grape coloring agent, under evaluation for apple and pear post-bloom thinning (and for ORGANIC production, as well!)

Improving Apple Color 2 applications each treatment 4 and 2 weeks before harvest

Untreated control Blush or Stimplex (4 + 2 WBH) No effect on fruit size No effect on fruit drop No effect in fruit quality (SS and firmness)

Apple Flower Development Foster et al., 2003

Stage 0 Vegetative stage Flat meristem Fotos: MEV. Barra: 100µm Source: Francescatto, P. 2014 Stage 1 Domed apex (meristem) Flower initiation

Flower initiation in apples Flower induction Flower initiation July DAFB October Fonte: Hoover et al. (2004)

Hormonal balance Production sites of flower inhibitors (GAs and Auxins) Floral inhibition GA 7 > GA 3 > GA 4 > seeds > flower inhibition GA 1 GA 3 GA 3 GA 4 > Shoot growth > Flower inhibition Flower induction Cytokinins (BA) Ethylene

Control of Biennal Bearing To promote return bloom of apple trees cvs. Honeycrisp and Fuji (and pears) Results to be analyzed in 2016 Small doses

Pre-harvest drop control: To study the effect of Retain and/or Harvista on fruit drop control and fruit quality; Variety: McIntosh Marshalls/M9 19 year old trees - % Fruit drop (from 9/14 (2WBCH) to 10/27; - Fruit quality at harvest (color, firmness, starch, sugar) 5-6 weekly picks; - Fruit quality after regular cold storage (firmness, sugar and disorders)

100 90 80 70 Application timing 4WBH 3WBH 2WBH 1WBH Avg Temp (F) - 2015 Avg Temp (F) - 2014 Mean Temperature (oc) 60 50 40 30 20 10 0 Harvest

1) Untreated control 2) 2 pouch Retain (4WBH) 3) 2 pouch Retain (3WBH) 4) 1 pouch Retain (4WBH) 5) 1 pouch Retain (3WBH) 6) 1 pouch Retain (4 + 2WBH) 7) 1 pouch Retain (3 + 1WBH) 8) ½ pouch Retain + 10ppmNAA (3 + 1WBH) 9) 60g i.a. Harvista (1WBH) 10) Harvista (10 days BH + 3 days BH) 11) ½ pouch Retain (3WBH) + Harvista (1WBH) Variety: McIntosh Marshalls Predicted Harvest 20 days 2,5weeks good firmness and fruit drop control

Department of Horticulture Cornell University, Geneva - NY Poliana Francescatto pf246@cornell.edu