Foreword: International Centers of Excellence for Malaria Research



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Accepted for Publication, Published online August 10, 2015; doi:10.4269/ajtmh.15-0407. The latest version is at http://ajtmh.org/cgi/doi/10.4269/ajtmh.15-0407 In order to provide our readers with timely access to new content, papers accepted by the American Journal of Tropical Medicine and Hygiene are posted online ahead of print publication. Papers that have been accepted for publication are peer-reviewed and copy edited but do not incorporate all corrections or constitute the final versions that will appear in the Journal. Final, corrected papers will be published online concurrent with the release of the print issue. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. RAO INTERNATIONAL CENTERS FOR EXCELLENCE IN MALARIA RESEARCH (ICEMR) Foreword: International Centers of Excellence for Malaria Research Malla R. Rao* Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland * Address correspondence to Malla R. Rao, Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, BG 5601Fl Room 8A38, 5601 Fishers Lane, Rockville, MD 20852. E-mail: mrao@niaid.nih.gov The past two decades have witnessed an extraordinary commitment to malaria control and elimination, which has been accompanied by a considerable reduction in global malaria morbidity and mortality. Although malaria research efforts have dramatically increased over this period, 1 most clinical research projects still tend to be narrowly focused in scientific scope, and conducted in a limited catchment area with a relatively homogeneous at risk population. In 2010, the National Institute of Allergy and Infectious Diseases established 10 International Centers of Excellence for Malaria Research (ICEMR) to support multidisciplinary research in diverse epidemiologic settings spanning representative malaria-endemic regions around the world. Table 1 describes the locations of field sites and research focuses of each ICEMR. Research projects were designed to capture and track changes in the complex interactions between the human host, malaria parasite, and mosquito vector in different eco-epidemiologic settings. Unlike typical monitoring and evaluation programs, the ICEMRs are mandated both to adopt an integrated approach to malaria research, and to apply molecular epidemiology and genomics to study transmission and disease in the context of dynamic changes in disease prevalence and incidence (Table 1). Following the initial establishment of the ICEMRs, Acta Tropica published a special supplement issue focusing on the ICEMRs, titled Tackling the Malaria End Game : Regional Needs and Challenges for Successful Malaria Elimination. 2,3 This supplement described each of the Centers, their research projects, and their scientific scope. The articles described how individual Centers planned to implement multidisciplinary research approaches in the context of local and regional control efforts to provide evidence-based findings to inform the future course of malaria control and elimination efforts. Taking advantage of the network structure of the ICEMRs, which encourages and facilitates collaboration between centers, the ICEMRs have shared protocols and technologies and have worked to harmonize outcome and predictor variable definitions. In this issue, the ICEMRs jointly present early findings organized based on broad themes rather than results specific to individual centers. This approach highlights the value of a broadly focused program and the generalizability of the findings from the ICEMR network. Copyright 2015 by the American Society of Tropical Medicine and Hygiene

In this research supplement, the ICEMRs attempt to provide a comprehensive view of the interplay between control interventions and epidemiology, vector ecology, parasite diversity, insecticide and drug resistance, pathogenesis, diagnostic performance, molecular epidemiology, biosignatures of transmission and immunity, and urban malaria across different settings. The impact of human migration, man-made ecological modifications, and climate and vector behavior on malaria transmission is extremely complex to study and quantify. The multidisciplinary nature of the ICEMR program enables each center to study these interactions in a specific, well-defined setting, and to subsequently compare findings across centers for consistency and variability. Table 2 provides an overview of the transmission settings in which the ICEMRs are located as well as study questions and approaches. Although the incidence of malaria continues to decline in many parts of the world, some sites have experienced little or no change, and a few locations have seen incidence rise. The most pressing scientific questions will differ depending on the transmission setting. For example, when transmission is low, how is it sustained and what control and surveillance measures will be most effective at driving rates toward elimination? Where transmission levels remain high despite interventions, 4 what are the factors or knowledge gaps that impede impact? Where malaria has recently dropped to low levels, what are the early warning signs of an impending epidemic? In addition to insecticide-impregnated bed nets and indoor residual spraying of insecticides, a number of novel control and prevention strategies have been proposed in recent years. Seasonal malaria chemoprophylaxis, mass drug administration, school-based treatment programs, targeted parasite elimination, and outdoor vector control are examples of a few approaches being tried in different settings. 5 7 In addition to the direct impact these interventions might have on disease and infection rates, it is critical to study their effects on the immune status of the population, the genetic diversity of circulating parasite strains, the evolutionary response of emerging and surviving strains, as well as impacts on the mix of vector species and their vectorial capacity. All of these factors will ideally be taken into account to maximize the likelihood of achieving malaria elimination as quickly as possible. As a network of multidisciplinary research centers in diverse epidemiological settings, the ICEMRs are poised to provide insightful research findings that will inform the design and implementation of optimal malaria control and elimination programs. Received June 2, 2015. Accepted for publication June 15, 2015. Author s address: Malla R. Rao, Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, E-mail: mrao@niaid.nih.gov. REFERENCES <eref>1. The Global Fund, 2013. Cumulative Signed Funding by Disease Table. Available at: http://www.theglobalfund.org/en/about/fundingspending/. Accessed May 12, 2015.</eref> <jrn>2. Rao M, 2012. Foreword. The International Centers of Excellence for Malaria Research. Acta Trop 121: 157.</jrn> <jrn>3. Volkman SK, Ndiaye D, Diakite M, Koita OA, Nwakanma D, Daniels RF, Park DJ, Neafsey DE, Muskavitch MA, Krogstad DJ, Sabeti PC, Hartl DL, Wirth DF, 2012. Application of genomics to field investigations of malaria by the International Centers of Excellence for Malaria Research. Acta Trop 121: 324 332.</jrn>

<jrn>4. Kamya MR, Arinaitwe E, Wanzira H, Katureebe A, Barusya C, Kigozi SP, Kilama M, Tatem AJ, Rosenthal PJ, Drakeley C, Lindsay SW, Staedke SG, Smith DL, Greenhouse B, Dorsey G, 2015. Malaria transmission, infection, and disease at three sites with varied transmission intensity in Uganda: implications for malaria control. Am J Trop Med Hyg 92: 903 912.</jrn> <jrn>5. Searle KM, Shields T, Hamapumbu H, Kobayashi T, Mharakurwa S, Thuma PE, Smith DL, Glass G, Moss WJ, 2013. Efficiency of household reactive case detection for malaria in rural southern Zambia: simulations based on cross-sectional surveys from two epidemiological settings. PLoS ONE 8: e70972.</jrn> <jrn>6. Van Roey K, Sokny M, Denis L, Van den Broeck N, Heng S, Siv S, Sluydts V, Sochantha T, Coosemans M, Durnez L, 2014. Field evaluation of picaridin repellents reveals differences in repellent sensitivity between southeast Asian vectors of malaria and arboviruses. PLoS Negl Trop Dis 8: e3326.</jrn> <jrn>7. Burkot TR, Russell TL, Reimer LJ, Bugoro H, Beebe NW, Cooper RD, Sukawati S, Collins FH, Lobo NF, 2013. Barrier screens: a method to sample blood-fed and host seeking exophilic mosquitoes. Malar J 12: 49.</jrn>

TABLE 1 ICEMR: description of centers ICEMR Locations/(sites) Title Grantee institution Research focuses Latin America Caucaseco Scientific Research Center, Cali Amazonia West Africa Southern Africa East Africa Malawi South Asia India Southeast Asia Southwest Pacific Colombia (Tierralta, Buenaventura, Tumaco, Quibdó) Peru (Sullana) Ecuador (Esmeraldas) Guatemala (Zacapa, Alta Verapaz, Escuintla) Peru (Loreto) Brazil (Remansinho, Acrelândia) Gambia (Gambissara) Senegal (Dakar) Mali (Dangassa, Dioro) Zambia (Nchelenge, Choma) Zimbabwe (Mutasa) Uganda (Jinja, Kanungu, Tororo) Blantyre, Chikwawa, Thyolo India (Goa, Wardha, Dibrugarh, Ranchi, Mumbai) Chennai, Rourkela, Nadiad China (Ying Jiang) Thailand (Tha Song Yang) Myanmar (Kachin State) PNG (East Sepik, Madang) Solomon Islands (Central and Western Provinces) Latin American Center for Malaria Research and Control Peruvian/Brazilian Amazon Center of Excellence in Malaria Population-Based Approach to Malaria Research and Control Malaria Transmission and the Impact of Control Efforts in southern Africa Program for Resistance, Immunology, Surveillance and Modeling of Malaria In Uganda Determinants of Malaria Disease in Malawi Malaria Evolution in South Asia Center for the Study of Complex Malaria In India Southeast Asia Malaria Research Center Research to Control and Eliminate Malaria in southeast Asia and southwest Pacific California, San Diego Tulane Louisiana Johns Hopkins Bloomberg School of Public Health California, San Francisco Michigan State University Washington New York University Pennsylvania State UniversityUniversity Park Case Western Reserve University Malaria in low transmission settings Role of asymptomatics in transmission and assessment of new tools for elimination Ecological diversity of vectors and parasites Clinical profile of complicated and uncomplicated malaria Biology of hypoendemic malaria Asymptomatic human, new seroepidemiological tools Ecology and environment related to malaria entomology Immunology of transmission to mosquitoes Impact of different control mix Immune responses Drug resistance Insecticide resistance Factors influencing transmission and control in regions with: Low-level endemicity approaching elimination Resurgence after successful control High transmission despite adequate control Transmission at sites with varying intensities Surveillance strategies Sero-epidemiology Drug and insecticide resistance Monitoring impact in diverse geographic settings Disease pathogenesis in severe malaria School-based interventions Urban Malaria Parasite evolution in diverse transmission settings Genome plasticity and drug resistance Pathogenesis mechanisms Innate human and vector protection Disease outcomes based on complexity of infection due to: Mixed species Multiple genotypes Varied ecologies (urban vs. forest) Different vectors Influence of cross-border migration on Epidemics of malaria Complexity of circulating genotypes Evolution of drug resistance Diagnostics for drug quality Impact of transmission reduction on Holoendemic malaria in mainland PNG Hypoendemic malaria in Solomon Islands Relative impact on Plasmodium vivax vs. Plasmodium falciparum

TABLE 2 Transmission setting Study questions Outcomes and measures ICEMRs Sites Perennial high level Reasons for failure and success of control measures New alternatives ICEMR: description of centers: research settings and questions Seasonal high Unpredictable Urban, mining, deforested, levels epidemics/reemerging malaria border, and remote areas Vector Contribution of immune status Features of environmental breeding modification sites and Changes in Changes in age and severity Detection of breeding sites immunity spectrum and Factors responsible for failure Contribution of migrants severity of control and human movement Species and strain diversity Insecticide Vector behavior and mix Human movement and migration New environments for vector breeding sites resistance Drug resistance Immune status Introduction of novel strains Risk behavior of populations Genetic factors of Asymptomatic Strain diversity and mix Vector detection and host collection Asymptomatic Immune markers of exposure and protection West Africa, southern Africa, East Africa, Malawi, south Asia, India, southwest Pacific Dangassa (Mali), Nchelenge (Zambia), Tororo and Kanungu (Uganda), Chikhwawa (Malawi), Dibrugarh and Rourkela (India), East Sepik and Madang (PNG) Gametocyte carriage Malawi, south Asia, India, southeast Asia Thyolo (Malawi), Ranchi and Rourkela (India), Kachin State (Myanmar) Treatments, regimens, and control strategies Latin America, Amazonia, southern Africa, East Africa, south Asia, southeast Asia Tumaco (Colombia), Esmeraldas (Ecuador), Loreto (Peru), Mutasa (Zimbabwe), Jinja (Uganda), Goa (India), Kachin State (Myanmar) ICEMR = International Centers of Excellence for Malaria Research. Socioeconomic status and malaria risk Latin America, Amazonia, West Africa, East Africa, Malawi, South Asia, India, southeast Asia Quibdó (Colombia), Loreto (Peru), Dakar (Senegal), Jinja (Uganda), Blantyre (Malawi), Goa, Diburgarh and Chennai (India), Thai Myanmar border, China Myanmar border Low-level approaching elimination Optimal surveillance strategies and diagnosis tools Optimal control strategies and mix Vector density Serologic markers Landscape genetics Submicroscopic Latin America, Amazonia, southern Africa, India, southwest Pacific Alta Verapaz (Guatemala), Buenaventura (Colombia), Sullana (Peru), Choma (Zambia), Nadiad (India), Central and Western Province (Solomon Islands)