Allergy Testing at Dorevitch Pathology

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Important changes to Allergy Testing at Dorevitch Pathology (03) 9244 0444 dorevitch.com.au

You may have noticed some changes to standard allergen-specific IgE (sige) testing panels and reporting in the last few months. Over the next few weeks we will be introducing further changes which will enable us to continue to provide the same high level of allergy testing service on the best available testing system, the Phadia ImmunoCAP. Interpretation of allergen-specific IgE tests Allergy is not an all-or-nothing phenomenon, and the development of an allergic response starts with initial sensitisation, at very low levels of sige. Later, as the level of sige increases, the patient may progress from asymptomatic sensitisation to presentation with clinical symptoms, e.g. symptoms of food allergy such as urticaria or anaphylaxis, or symptoms of inhalant allergy such as rhinoconjunctivitis or asthma. In clinical practice, 0.35 kua/l has commonly been used as a cut-off for allergen-specific IgE to separate positive from negative results. Nevertheless, a positive allergen-specific IgE test (which indicates detection of allergen-specific IgE) does not necessarily indicate clinical allergy. In general, low sige antibody levels indicate a low probability of clinical disease, whereas high antibody levels to an allergen show good correlation with clinical disease. Clinical history is the best indicator of possible allergy. A positive allergen specific IgE is used together with the clinical history to support and confirm the diagnosis of clinical allergy. Allergen exposure Allergic sensitisation > > Allergic disease

Changes to standard allergy testing panels. For requests that state RAST test or allergy test without stipulating specific allergens of interest, we will run a standard panel of tests that covers the common allergens (Australian grass and weed pollens, and house dust mite) which are relevant to the most common allergic disorders (asthma, allergic rhinitis). We encourage clinicians to specify allergens of interest when ordering allergy tests. This change took effect in May 2013. If there is a clinical suspicion of allergy to an allergen(s) that has not been tested, serum will be held for 7 days in the event that add-on testing for additional allergen specific IgE is required. Bulk billing of allergy testing. Medicare-compliant tests will be bulk-billed. If requests contain more allergens than are covered by the allowance under Medicare (i.e. more than 4 allergens or allergen mixes per request or more than 4 episodes of allergy testing within a period of 12 months), patients will be subject to an out-of-pocket charge as summarised below (Table 1). Table 1: Gap payment scenarios for allergy testing at Dorevitch Pathology. SCENARIO GAP PAYMENT COMMENTS > 4 allergens or mixes/episode $40.00 per episode Non-specialist referrals only > 4 episodes/12 months Recombinant or component allergens $60.00 per episode $40.00 per episode

Introduction of molecular allergy testing. Dorevitch Pathology is now offering a broad array of molecular allergy tests. Component-resolved diagnosis, based on well-characterised individual allergen molecules, is a major advance in allergy diagnosis, allowing prediction of the nature and severity of allergic reactions, and symptoms due to allergen cross-reactivity. These specialised recombinant and component allergens are unfortunately expensive, so in order to provide a comprehensive repertoire of these useful tests, out-of-pocket charges will be introduced for each allergen requested. Examples of these allergens are listed in Table 2. Table 2: Recombinant and component allergens subject to out-ofpocket charges ($40 per allergen) CODE NAME COMMON REQUEST TERM f232 Egg (ngal d 2, Ovalbumin) ovalbumin f233 Egg (ngal d 1, Ovomucoid) f76 Milk (nbos d 4, α-lactalbumin) f77 Milk (nbos d 5 B- lactoglobulin) f423 Peanut (rara h 2) f416 Wheat (rtri a 19, Omega-5 Gliadin) ovomucoid lactalbumin lactoglobulin Ara h 2 omega 5 gliadin

Allergen - Specific IgE (sige) Available. The following allergen-specific IgE tests are readily available. Note: sige to allergen mixes are available clearly state components of mix to ensure the correct test is performed. Additional tests for single allergens and allergen mixes are also available - please contact Special Chemistry on (03) 9244 0326 Mon-Fri 8am-4pm for further information or contact the clinical allergist to discuss. Medicare rebate only covers the cost of three to four standard sige tests (excludes component sige tests) per request. DRUGS Amoxicilloyl Ampicilloyl Cefaclor Chlorhexidine Gelatin bovine Morphine Penicilloyl G* Pholcodeine Suxamethonium MITES Blomia tropicalis D.farinae D.pteronyssinus Glycyphagus domesticus (Storage mite Dust mix (Hollister-Stier labs, D. pteronyssinus, D. farinae, cockroach) ANIMALS Cat epithelium & dander Dog dander Guinea Pig Horse dander Mouse epithelium Mouse Urine Proteins Rabbit epithelium Animal mix (Danders: cat, horse, cow, dog) FOODS Abalone Almond Asparagus Avocado Banana Barley Beef Blue mussel Buckwheat Cashew Chicken Clam Clove Crab Crayfish Egg white Fish (cod) Goat s milk Hazelnut Kiwi Lentil Lobster Lupin Macadamia Mackerel Maize, corn Milk (Cow s milk) Mutton Oat Octopus Oyster Pea Peanut Pecan Pine nut Pistachio Pork Potato Pumpkin Quinoa rara h 2 Peanut Red Snapper Rice Rye Salmon Sardine Scallop Sesame seed Sheep milk Shrimp Soya bean Squid Sweet potato Trout Tuna Turkey Meat Walnut Wheat White fish Yeast Cereal mix (wheat, oat, maize, sesame seed, buckwheat) Citrus mix (orange, lemon, grapefruit, mandarin) Meat mix (pork, beef, chicken) *Nut mix 1 (peanut, hazel, brazil, almond, coconut) *Nut mix 2 (pecan, cashew, pistachio, walnut) Seafood mix (cod fish,shrimp, blue mussel, tuna, salmon) Staple food mix (egg white, milk, fish, wheat, peanut, soybean) *This detects sige against the major determinant Benzyl penicilloyl. sige to minor determinants not available.

INSECTS Cockroach European Paper Wasp Honey bee Yellow jacket (European or common) MISCELLANEOUS Anasakis Latex rhev b 5 Latex MOULDS Alternaria alternata Aspergillus fumigatus Cladosporium herbarum Mould mix 1 (penicillium chrysogenum, Cladosporium herbarum, Aspergillus fumigatus, Alternaria alternanta) GRASSES Bahia grass Bermuda grass (Couch) Rye grass Timothy grass Aust pollen mix (bermuda,rye, bahia, common ragweed, plantain, lamb s quarters) Grass mix (bermuda, rye, timothy, kentucky, johnson, bahia) TREES Acacia Aus pine (Casuarina eq.) Cedar Cypress Eucalyptus European Ash Maple leaf sycamore, London Plane Melaleuca Cajeput-tree Olive Pine Privet Silver birch White Pine Willow Tree mix 1 (Box-elder, Common silver birch, oak, walnut, Elm) Tree mix 2 (Box-elder, oak, Elm,Cottonwood, Pecan/Hickory) Aust tree mix (Olive, Eucalyptus, Acacia, willow, white pine, melaleuca) WEEDS Common Ragweed Plantain Weed mix (common ragweed, mugwort, plantain, lamb s quarters, saltwort) List correct as of July 2015 For any further enquiries regarding these changes, please contact: Dr Mimi Tang Immunopathologist, Clinical Immunologist and Allergist, (03) 9244 0326 Specialist Diagnostic Services, ABN 84 007 190 043 APA No. 000042, trading as Dorevitch Pathology. DP0286 17.08.15