INNOVATIONS IN RECIRCULATING INLAND MARINE AND FRESHWATER FISH PRODUCTION SYSTEMS

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INNOVATIONS IN RECIRCULATING INLAND MARINE AND FRESHWATER FISH PRODUCTION SYSTEMS Kevan L. Main, Jim Michaels, Michael Nystrom Mote Marine Laboratory, Sarasota, Florida, USA & Thomas Losordo North Carolina State University, Raleigh, North Carolina, USA

Outline of Presentation Overview of Mote Aquaculture Park Research Programs, Facilities & Systems Overview of Denitrification Research Freshwater & Marine Prototype System for Fingerling Production System overview & Preliminary Operation

Aquaculture Research at Mote Focused on Developing & Demonstrating Recirculating Aquaculture System (RAS) Technology Concerns about pollution of marine & freshwater resources High cost of coastal land Limited water resources Efficient use of water is needed to sustainability grow commercial aquaculture in the U.S.

Mote Aquaculture Park: 2009 Maintenance & Shop Storage Sturgeon GO-III Sturgeon Hatchery & Office Sturgeon GO-I Sturgeon GO-II Marine Broodstock Marine Fish Hatchery Sturgeon Processing Marine Filtration Sturgeon Purge Red Drum Hatchery Snook Pond

Sturgeon Commercial Demonstration Program Demonstrate commercial viability of sustainable aquaculture farming in Florida Develop innovative culture techniques to protect threatened sturgeon stocks Develop technologies to conserve limited water resources

Larval Rearing Tanks & Filtration

Fingerling Tanks & Filtration

Sturgeon Growout Tanks

Solids Filtration Microscreen Drum (removes particles/solids greater than 60 microns)

Biofiltration Moving Bed Reactor (nitrification chamber)

Degassing Tank CO2 Stripping

Oxygenation

Marketing Sustainable Seafood Products to U.S. Consumers Siberian Sturgeon & Caviar from Mote Aquaculture Park Fish You Can Feel Good About

Marine Aquaculture Research Program Design & test zero-discharge, sustainable marine farming systems for inland applications Demonstrate the use of these technologies to culture marine fish (pompano, snook, red drum) and invertebrates (hard corals, sea urchins) for food & stock enhancement

Marine Fish Breeding Systems Large breeding tanks Independent temperature, lighting and filtration

Marine Fish Broodstock System Small Scale Moving Bed Reactor Foam Fractionator Drop Filter for Solids Removal UV Filter 4/17/2009

Marine Hatchery/Nursery Facilities

Denitrification Research Freshwater Phase I Evaluate the performance of the denitrification reactor Evaluated 4 carbon sources (vinegar, molasses, starch, methanol) for viability and comparative performance Phase II Determined that molasses was the most viable carbon source option under commercial conditions in our location Capital cost savings were 27% higher & operating cost savings were 87% higher for molasses vs. other carbon sources Marine Phase I Trial Evaluate the efficiency of a denitrification reactor in removing nitrate in a low salinity (15-18 ppt) wastewater treatment system Carbon source Methanol Maximum water flow rate = 6 L/min: 12 ml/min carbon (15% methanol)

Commercial-Scale Freshwater Denitrification Filter Carbon Sources

Preliminary Results Denitrification Filter Trial Low-Salinity Wastewater Treatment System Carbon Source (Methanol Tank) Biofilte Tank Denitrification Reactor 160 140 120 Started methanol flow @ 2ml/min Increased water flow to 2L/min Increased water flow to 3L/min Increased methanol flow to 6ml/min During this time the pump was slowly decreasing due to a clo and reduced the flow to 3.0L/min NO3 mg/l 100 80 60 Started Water flow @ 1L/min Increased water flow to 3.8L/min Water flow was restored to 4.0L/min NO3 Before Reactor NO3 Post Reactor Denitrification Reactor Outlet 40 20 0 0 1 2 3 4 5 6 7 8 9 10 11 12 13 Weeks

Florida Marine Fisheries Enhancement Initiative Designing, constructing & evaluating system performance of a prototype system to produce fingerling fish for stock enhancement Evaluating Red Drum performance (growth, survival, health) in intensive recycle systems

Prototype Zero- Discharge Fingerling Production System

Filtration System Components

Wastewater Treatment Components

Acknowledgements FL Department of Agriculture & Consumer Services Division of Aquaculture USDA/CSREES FL Fish and Wildlife Conservation Commission Southwest Florida Water Management District Darden Restaurants Foundation Mote Scientific Foundation