2 Molecular Biology Kits

56 BIO BASIC INC. www.biobasic.com Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Plasmid DNA, Gel Extraction & PCR Products Purification Kits Code Description Size B60363(BS363) EZ-0 Spin Column PCR Products Purification Kit $ 36.75 B60363(BS364) EZ-0 Spin Column PCR Products Purification Kit 00 Preps $ 63.00 B630663(BS664) B80365(BS365) EZ-0 Spin Column PCR Products Purification Kit 96-Well Plate PCR Products Purification Kit XPlates $ 47.00 $ 3.5 B80365(BS365) B60367(BS367) B60367(BS368) B60367(BS668) B80369(BS369) B80369(BS369) B60353(BS353) B60353(BS354) B630653(BS654) B6043(BS439) 96-Well Plate PCR Products Purification Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit 96-Well Plate DNA Cleanup Mini-preps Kit 96-Well Plate DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column Plasmid DNA Mini-preps Kit 5 X Plates 00 Preps XPlates 5 X Plates 00 Preps $ 6.50 $ 36.75 $ 63.00 $ 47.00 $ 3.5 $ 6.50 $ 36.75 $ 63.00 $ 47.00 $ 36.75 B6043(BS449) EZ-0 Spin Column Plasmid DNA Mini-preps Kit 00 Preps $ 63.00 B6043(BS649) EZ-0 Spin Column Plasmid DNA Min-preps Kit $ 47.00 B604(BS804) Ultra-Fast Spin Column Plasmid DNA Min-Preps Kit $ 5.50 B60465(BS4654) EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 4 Preps $ 75.60 B60465(BS466) EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 0 Preps $ 378.00 B8045(BS45) 96-Well Plate Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B8045(BS45) 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B8008(BS908) Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B8008(BS909) Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B80043(PT9043) 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B80043(PT9044) 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B698(BS798) EZ-0 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit $ 0.90 B549(SK49) EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 4 Maxi-Preps $ 90.7 B549(SK50) EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 0 Maxi-Preps $ 453.60 B60467(BS467) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit 0 Preps $.00 B60467(BS468) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit $ 84.00 B60467(BS469) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit $ 336.00 Genomic DNA Isolation Kits Code Description Size A8080(BS80) EZ-DNA Reagents 00 Preps $ 5.50 B80843(BS843) One-Tube Bacteria Genomic DNA Isolation Kit 00 Preps $ 39.90 B80843(BS844) One-Tube Bacteria Genomic DNA Isolation Kit 500 Preps $ 59.60 B585(BS85) Rapid Bacteria Genomic DNA Isolation Kit $ 36.75 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 57

B6043(BS43) EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit $ 44.0 B80064(BS64) EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit $ 83.75 B809(SK9) 96-Well Plate Bacteria Genomic DNA Mini-Preps Kit X Plates $ 59.60 B8095(SK95) 96-Well Plate Bacteria Genomic DNA Mini-Preps Kit 5X Plates $ 365.40 B587(BS87) Rapid Yeast Genomic DNA Extraction Kit $ 36.75 B80857(BS857) EZ-0 Spin Column Yeast Genomic DNA Mini-Preps Kit $ 60.90 B808357(BS8357) B8045(FT745) B800(FT80) B80037(PT9037) B8086(PT786) B8086(PT787) B80045(BS45) B80045(BS66) B80035(PT9035) B80035(PT9036) 96 Well Plate Yeast Genomic DNA Mini-Preps Kit Rapid Fungal Genomic DNA Isolation Kit EZ-0 Spin Column Fungal Genomic DNA Mini-Preps Kit EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit One-tube Plan Genomic DNA Extraction Kit One-tube Plan Genomic DNA Extraction Kit EZ-0 Spin Column Plant Genomic DNA Mini-Preps Kit EZ-0 Spin Column Plant Genomic DNA Mini-Preps Kit EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit X Plates 4 Preps 00 Preps 4 Preps 0 Preps $ 86.90 $ 36.75 $ 44.0 $ 86.0 $ 5.50 $ 0.00 $ 44.0 $ 83.75 $ 86.0 $ 344.40 B80836(BS836) 96-Well Plate Plant Genomic DNA Mini-Preps Kit X Plates $ 86.90 B8036(ST836) EZ-0 Spin Column Soil DNA Mini-Preps Kit $ 44.0 B5840(BS840) One-Tube Tissue DNA Extraction Kit 00 Preps $ 39.90 B5840(BS840) One-Tube Tissue DNA Extraction Kit 500 Preps $ 59.60 B804780(AT4780) Rapid Animal Genomic DNA Extraction Kit 0 Preps $ 6.5 B804780(AT478) Rapid Animal Genomic DNA Extraction Kit $ 05.00 B804780(AT478) Rapid Animal Genomic DNA Extraction Kit $ 40.00 B80047(BS47) EZ-0 Spin Column Animal Genomic DNA Mini-Preps Kit $ 44.0 B80047(BS68) EZ-0 Spin Column Animal Genomic DNA Mini-Preps Kit $ 83.75 B80034(PT9034) EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit 4 Preps $ 86.0 B80437(BS437) 96-Well Plate Animal Genomic DNA Mini-Preps Kit X Plates $ 86.90 B80437(BS437) 96-Well Plate Animal Genomic DNA Mini-Preps Kit 5X Plates $ 47.90 B58403(BS8403) One-Tube Clinical Sample DNA Extraction Kit 00 Preps $ 39.90 B58403(BS8404) One-Tube Clinical Sample DNA Extraction Kit 500 Preps $ 59.60 B80478(BT478) Rapid Blood Genomic DNA Extraction Kit $ 36.75 B80483(BS483) EZ-0 Spin Column Blood Genomic DNA MiniPreps Kit $ 44.0 B80483(BS684) EZ-0 Spin Column Blood Genomic DNA MiniPreps Kit $ 83.75 B8003(BT903) 96 Well Plate Blood Genomic DNA Mini-Prep Kit Plates $ 59.60 B8003(BT903) 96 Well Plate Blood Genomic DNA Mini-Prep Kit 5 Plates $ 365.40 B58409(VT4785) One Tube Viral DNA Isolation Buffer $ 5.50 B58409(VT4786) One Tube Viral DNA Isolation Buffer 00 Preps $ 0.00 B5867(VT88) EZ-0 Spin Column Viral DNA Mini-Preps Kit $ 7.40 B5867(VT883) EZ-0 Spin Column Viral DNA Mini-Preps Kit $ 357.00 B8003(VT903) 96-Well Plate Viral DNA Mini-Preps Kit X Plates $ 43.60 B58405(T76) One-Tube Swab DNA Extraction Kit 0 Preps $ 3.0 58 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue B58405(T763) One-Tube Swab DNA Extraction Kit 00 Preps $ 68.5 B58405(T764) One-Tube Swab DNA Extraction Kit 500 Preps $ 73.00 B58407(BS8407) One-Tube Hair DNA Extraction Kit $ 39.90 B68503(BS88503) ONE-4-ALL Genomic DNA Miniprep Kit $ 9.40 B68503(BS88505) ONE-4-ALL Genomic DNA Miniprep Kit DNA/RNA/Protein Isolation Kit $ 369.60 Code B68003(BS88003) B6803(BS8803) RNA Isolation Kit Code B800409(BS409A) B800409(BS400A) B536(BS36) B5865(BS865) Description All-In-One DNA/RNA/Protein Miniprep Kit All-In-One DNA/RNA Miniprep Kit Description One Step RNA Reagent One Step RNA Reagent EZ-0 Spin Column Total RNA Mini-preps Kit Rapid Bacteria RNA Isolation Kit Size Size 5 ml 00 ml $ 0.5 $ 99.75 $ 6.5 $ 84.00 $ 60.90 $ 40.95 B60583(BS583) EZ-0 Spin Column Total RNA Mini-Preps Super Kit 0 Preps $ 36.75 B60583(BS584) EZ-0 Spin Column Total RNA Mini-Preps Super Kit 00 Preps $ 57.50 B60583(BS784) EZ-0 Spin Column Total RNA Mini-Preps Super Kit $ 35.00 B80585(BS585) 96 Well Plate Bacterial Total RNA Mini-Preps Super Kit X Plates $ 6.50 B80585(BS585) 96 Well Plate Bacterial Total RNA Mini-Preps Super Kit 5X Plates $ 590.0 B635(BS935) EZ-0 Spin Column RNA Cleanup & Concentration Kit $ 68.5 B805(BRC5) 96 Well Plate RNA Cleanup & Concentration Kit XPlates $ 6.50 B8046(FT746) Rapid Fungal RNA Extraction Kit $ 40.95 B695(BS995) EZ-0 Spin Column Fungal RNA Mini-Preps Kit $ 78.75 B8049(PT49) Rapid Plant RNA Isolation Kit $ 40.95 B634(BS834) EZ-0 Spin Column Plant RNA Mini-Preps Kit $ 78.75 B58637(VT484) Rapid Viral RNA Extraction Kit $ 7.40 B58667(VT8) EZ-0 Spin Column Viral Total RNA Extraction Kit $ 06.05 B8048(AT48) Rapid Animal Total RNA Extraction Kit $ 5.50 B8048(AT48) Rapid Animal Total RNA Extraction Kit $ 0.00 B63(BS83) EZ-0 Spin Column Animal Total RNA Mini-Preps Kit $ 65.0 B63(BS83) EZ-0 Spin Column Animal Total RNA Mini-Preps Kit $ 60.40 B8048(BT48) Rapid Blood RNA Isolation Kit 0 Preps $ 44.0 B8048(BT483) Rapid Blood RNA Isolation Kit $ 57.50 B8048(BT484) Rapid Blood RNA Isolation Kit $ 630.00 B633(BS833) EZ-0 Spin Column Blood RNA Mini-Preps Kit $ 78.75 B8098(MT998) mrna Purification Kit from Total RNA 5 Preps $ 0.90 B68583(BS88583) EZ-0 Total RNA Miniprep Kit $ 78.75 B6853(BS8853) Rnase-Free DNAse Set $ 3.30 B6833(BS8833) EZ-0 DNAway RNA Miniprep Kit $ 0.5 B6833(BS8836) EZ-0 DNAway RNA Miniprep Kit $ 44.00 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 59

More Kits Code Description Size A8000(9K-006-000) Gel Extraction Kit (00 preps) 00 Preps $ 4.00 A8000(9K-006-000) Gel Extraction Kit (00 preps) 00 Preps $ 343.00 A830003(9K-006-0003) A830003(9K-006-0004) A80009(9K-006-0009) PCR Purification Kit (00 preps) PCR Purification Kit (00 preps) Plasmid Miniprep Kit (00 preps) 00 Preps 00 Preps 00 Preps $ 00.00 $ 300.00 $ 79.00 A80009(9K-006-000) Plasmid Miniprep Kit (00 preps) 00 Preps $ 57.00 A80003(9K-006-003) Genomic DNA Extraction. Blood Genomic DNA Prep Kit (400Rx) 400 Rxn $ 307.00 A8004(9K-006-004) Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (400Rx) 400 Rxn $ 350.00 A80005(9K-006-005) Genomic DNA Extraction. Plant Genomic DNA Prep Kit (400Rx) 400 Rxn $ 350.00 A8006 (9K-006-006) Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn $ 350.00 A80007(9K-006-007) Yeast Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn $ 350.00 A80003(9K-006-008) Genomic DNA Extraction. Blood Genomic DNA Prep Kit (3300Rx) 3300 Rxn $ 364.00 A8004(9K-006-009) Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (3300Rx) 3300 Rxn $657.00 A80005(9K-006-000) Genomic DNA Extraction. Plant Genomic DNA Prep Kit (3300Rx) 3300 Rxn $8.00 A8006 (9K-006-00) Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn $8.00 A80007(9K-006-00) Yeast Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn $8.00 A8003(9K-006-003) Plasmid DNA Maxiprep (0 preps) 0 Preps $89.00 A8003(9K-006-007) Plasmid DNA Midiprep (5 preps) 5 preps $398.00 Columns & 96 Well Plates Code Description Size B65005(SD5005) EZ-0 Column & collection tube (blue tube, clear ring, clear collection) 00 $ 4.5 B65005(SD5005-EMPTY) EZ-0 Empty Column without filter, without collection tube & O-ring 000 $ 5.50 B65008(SD5008) EZ-0 RNA Column & collection tube(clear tube, clear ring, clear collection) 00 $ 6.5 B65006(SD5006) 96 well filter plate (960μl each well) $ 37.80 B65006(SD5006-EMPTY) 96 well filter plate (no filter) $ 5.0 B65007(SD5007) 96 well DNA plate with membrane (960μl each well) $ 89.00 F80896(BR58-96N) 96 WELL ML DEEP PLATE, NATURAL,EDGE FILLED, 0PLATES/BAG PK $ 7.00 60 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

Bio Basic Inc. EZ-Kits Overview BBI 05-07 Catalogue Bio Basic Inc. supplies a remarkable variety of molecular biology kits, including Plasmid Miniprep, Gel Extraction, PCR Product Purification, and a complete line of Genomic DNA and RNA isolation kits offered in both spin column and 96-well plate formats! Although the detailed protocol for each kit varies, the general three-step principle remains the same: () BIND, () WASH, (3) ELUTE. Bio Basic s EZ-Membrane allows for fast and efficient purification, regardless of sample type, thereby providing you with high yields of purified product with minimal handling and effort. Spin Column Format EZ- 96-Well Plate Format Starting material Bind Wash () BIND Starting material is bound to Bio Basic s EZ-Membrane in spin column or plate format. () WASH The bound material is washed to remove impurities, buffers, and other unwanted materials. (3) ELUTE The purified product (DNA/RNA/Protein) is eluted from the membrane and collected for downstream application! Starting material Bind Wash Elute Purified product Elute Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 6

Plasmid DNA, Gel Extraction & PCR Products Purification Kits EZ-0 Spin Column PCR Products Purification Kit This kit utilizes membrane technology which selectively adsorbs up to 0 μg of PCR products or DNA fragments in the presence of a specialized binding buffer. Primers (<40-mer), nucleotides, enzymes, mineral oil and all other impurities are unable to bind and are eliminated from the column. PCR products or DNA fragments are eluted readily in elution buffer. PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. The kit can also be used for concentrating DNA fragments as well as removal of impurities such as salts and proteins.. Fast. Using a rapid spin column format, the entire procedure take less than 0 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B60363(BS363) B60363(BS364) B630663(BS664) 00 Preps Buffer B3 4 ml X4 ml 5X4 ml Wash Solution ml 4 ml 60 ml Elution Buffer 5 ml 0 ml 5 ml EZ-0 Column 50 00 50.0ml Collection Tube 50 00 50 Gel electrophoresis of PCR products purified using EZ-0 Spin Column PCR Products Purification Kit. $36.75 $63.00 $47.00 96-Well Plate PCR Products Purification Kit This kit provides a simple and efficient high throughput technique for the cleanup of PCR products and DNA fragments from gel extraction and enzymatic reaction solutions. PCR products or enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted with a small volume of low-salt elution buffer or water, and is ready for downstream applications including DNA sequencing and cloning.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure take less than 30 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Buffer B3 B80365 (BS365) x 96 well Plates 60 ml B80365(BS365) 5 x 96 well Plates X75 ml EZ-0 96-Well Plate 5 Deep Well Collection Plate 4 0 96 Storage Plate 5 Sealing film 8 0 Complete $3.5 $6.50 3456--------------------------5 Reproducible yields of PCR products purified using the 96-Well Spin Column PCR Products Purification Kit. Lane : DNA marker. Lanes -5: Crude PCR products. Lanes 6 to 5: Purified PCR products. Recoveries and yields are approximately 80%. 6 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue EZ-0 Spin Column DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient method for the purification of DNA fragments from various enzymatic reactions, including cdna synthesis, restriction digestions, and PCR, among others. It is also well-suited for desalting a solution of DNA, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures. The kit utilizes spin column technology which selectively adsorbs up to 0μg of DNA in the presence of specialized binding buffers. All impurities that do not bind are eliminated from the column. DNA fragments can be eluted readily with elution buffer. Purified DNA fragments and PCR products can be used for most downstream applications including sequencing and cloning.. Fast. Using a rapid spin column format, the entire procedure takes less than 0 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications, including digestion, cloning and sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. B60367(BS367) B60367(BS368) 00 Preps EZ-0 Column 50 00 50.0ml Collection Tube 50 00 50 $36.75 $63.00 $47.00 96-Well Plate DNA Cleanup Mini-Preps Kit B60367(BS668) Gel electrophoresis of PCR products purified using EZ-0 Spin Column DNA Cleanup Mini-preps Kit. This kit provides a simple and efficient high throughput method for the cleanup of PCR products and other DNA fragments from gel extraction solutions and enzymatic reactions. The kit utilizes a simple bind-wash-elute procedure. PCR products or other enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Whereas DNA binds to the column, impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA can be eluted in a small volume of the low-salt elution buffer or water, ready for use in all downstream applications including DNA sequencing and cloning. Phenol/chloroform extraction and ethanol precipitation are not required. Recovering yield is typically 60-80%. Up to 0 μg of purified DNA fragments in each well can be obtained. It is suitable to recover 00 bp-40 kb DNA fragments.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes less than 30 minutes.. High recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B80369 (BS369) x 96 well Plates B80369(BS369) 5 x 96 well Plates Clean Up Solution 60 ml X75 ml Elution Buffer ml 30 ml EZ-0 96-Well Plate 5 Deep Well Plate 4 0 96-Well Storage Plate 5 Sealing film 8 $3.5 0 $6.50 Reproducible yields of high quality PCR products purified using the EZ-0 96-well Plate DNA Cleanup Mini-Prep Kit. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 63

EZ-0 Spin Column DNA Gel Extraction Kit This kit provides a simple and rapid method for the purification of DNA fragments from agarose gel. It is also well-suited for desalting, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures containing DNA. The kit utilizes a spin column technology which selectively adsorbs up to 0 μg of DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the column and are eliminated. DNA fragments can be eluted readily in elution buffer and used for DNA sequencing, restriction digestion, and other downstream applications. Recovering yield ranges from 60 to 80%. No phenol/chloroform extraction or ethanol precipitation is required.. Fast. Using a rapid spin column format, the entire procedure takes less than 5 minutes.. High Recovery. Yield ranges from 60 to 80%. 3. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B60353(BS353) B60353(BS354) B630653(BS654) 00 Preps EZ-0 Column 50 00 50.0ml Collection tube 50 00 50 DNA fragments purified from an agarose gel using the Gel Extraction Kit. $36.75 $63.00 $47.00 EZ-0 Spin Column Plasmid Mini-Preps Kit This kit provides a simple and efficient method for the purification of plasmid DNA from bacterial culture. Plasmid DNA is selectively adsorbed by the EZ-0 column, while impurities such as proteins, salts and nucleotides are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator VisualLyse - is added to the buffer, which prevents insufficient or over-lysis during the lysis and neutralization step. Note: This kit is designed for the preparation of up to 0 μg of plasmid DNA. If larger amounts are required, plasmid Maxi preps are recommended. For low copy plasmid, B6043(BS439), B6043(BS449) and B6043(BS649) are recommended. Solutions are doubled in low copy plasmid kits.. Fast. Using a rapid spin column format, the entire procedure takes only minutes.. High Purity of DNA. OD 60 /OD 80 ratio of purified DNA is.8-.9, and can be used directly in most downstream applications such as sequencing, PCR, transformation, restriction digestion and ligation. 3. Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 4. Highest yield is guaranteed by using VisulLyse reagent. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B6043(BS43) B6043(BS44) 00 Preps B6043(BS64) EZ-0 Column 50 00 50.0ml Collection Tube 50 00 50 $36.75 $63.00 $47.00 *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 0 C. ** Before use for the first time, add four volumes of 96%-00% ethanol to Wash Solution (v/v). Gel electrophoresis of plasmid DNA purified using the EZ-0 Plasmid DNA Mini-preps Kit. 64 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Ultra-Fast Spin Column Plasmid DNA Mini-Preps Kit This kit facilitates single-step plasmid DNA purification from bacterial cultures. Using a one buffer system as opposed to the traditional three, the kit releases plasmid DNA rapidly from your sample. Although the yield of the recovered plasmid DNA is slightly lower than that from regular kits, it is much faster and simpler to use. Bacterial cultures are lysed and the lysates are directly loaded on to the spin column. Plasmid DNA is selectively bound onto the EZ-0 column and other impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water, and can be readily used for many downstream applications. This kit is suitable for the preparation of up to 8μg of pure plasmid DNA.. Fast and Simple: One step lysis of cell cultures. The entire procedure takes less than 0 minutes.. High Purity of DNA: OD 60 /OD 80 of plasmid DNA purified using this kit is >.8. Purified plasmid DNA can be used for many downstream applications, including DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic: phenol/chloroform extraction and ethanol precipitation are not required. 4. Direct manufacturer with most competitive price advantage. Transportation: At ambient room temperature. Lysis Buffer UF Buffer DW 4 ml Universal Wash Solution ml Elution Buffer 5 ml EZ-0 Column 50.0ml Collection Tube 50 $5.50 B604(BS804) 50 ml EZ-500 Spin Column Plasmid DNA Maxi-Preps Kit Plasmid DNA purified using the Ultra-Fast EZ-0 Spin Column Plasmid DNA Mini-preps Kit. This kit provides a simple and efficient method for purifying large amounts of plasmid DNA. Up to 500 μg of plasmid DNA is selectively adsorbed on the 5 ml EZ-500 column. Impurities, including proteins, salts and nucleotides, are eliminated from the column and washed away. Plasmid DNA can be eluted in 3 ml of Elution buffer. No phenol/chloroform or ethanol precipitations are required. DNA purified using the kit can be used in many downstream applications including transformation, DNA sequencing, PCR, and Southern-blotting. In order to obtain a maximal recovery yield, a color indicator - VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Specification: Sampling Yield Handling Time 00~50 ml of bacteria culture for high copy plasmids up to 500 µg ~ hours 00~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. B60465(BS4654) B60465(BS466) 4 Preps 0 Preps MAXI Resuspension Solution 55 ml X0 ml MAXI Cell Lysis Solution 55 ml X0 ml MAXI 3 Neutralization Solution 55 ml X0 ml MAXI 4 Equilibration Solution 54 ml X35 ml MAXI 5 Washing Solution 4X55 ml 4X75 ml MAXI 6 Elution Solution 54 ml X35 ml RNase A (50 mg / ml) 88 μl 440 μl MAXI Column 4 0 Collection Tube 4 0 $75.60 $378.00. Solutions provided in this kit contain irritants, wear gloves and lab coat when handling.. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI Resuspension Solution bottle. Add 50 μl of MAXI Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI Resuspension Solution bottle. Store at 4 C. 3. Check MAXI Cell Lysis Solution before use. Warm MAXI Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI Cell Lysis Solution. 4. To avoid acidification of MAXI Cell Lysis Solution from CO in the air, close the bottle immediately after use. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 65

96-Well Plate Plasmid DNA Mini-Preps Kit This kit provides a rapid, high-throughput method for 96-well plasmid DNA purification. Plasmid DNA is selectively adsorbed on silica membranes in the 96-well plate. Proteins, salts, nucleotides and other contaminants are subsequently eliminated from the column. Purified plasmid DNA can then be eluted in a small volume of elution buffer. In order to obtain maximal yield, a color indicator VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Purified DNA can be used directly in a variety of applications including sequencing, PCR and Southern-blotting. Note: This kit is used for the preparation of a maximum of 0μg of pure plasmid DNA per well.. Fast. Using the rapid spin column and high-throughput format, the entire procedure takes only 30 minutes.. High DNA Yield. Up to 0 μg DNA in each well can be obtained. 3. High Purity of DNA. OD 60 /OD 80 ratio of purified DNA is typically.8-.9. 4. Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. Solution I Solution III B8045 (BS45) 96 Well Plates 5 ml 90 ml B8045(BS45) 5 96 Well Plates 60 ml 0 ml EZ-0 96-Well Plate Deep Well Collection Plate 96 Storage Plate Sealing film 0 5 $3.5 $6.50 4 *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 C. ** Before use for the first time, add four volumes of 96%-00% ethanol to Wash Solution. 5 0 5 Reproducible yields of plasmid DNA purified using the 96-Well Plate Plasmid DNA Mini-preps Kit. Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit This kit is designed for one-step plasmid DNA purification from bacterial culture using a rapid and high throughput spin column format. Using a one buffer solution and replacing the traditional three, the kit releases plasmid DNA rapidly from bacterial culture. Although the yield of the recovered plasmid DNA is slightly lower than that from regular 96-Well Plate Kits, it is simpler and faster. Bacterial cultures are lysed and the lysates are directly loaded to each column of the 96-Well plate. Plasmid DNA is selectively bound onto the 96-well plate and other impurities are washed away. Pure plasmid DNA is eluted in Elution buffer or water, and can be readily used for most downstream applications. This kit is used for preparation of up to 6-8μg of pure plasmid DNA.. Fast & Simple. Using one step lysis spin column and 96-well high throughput format, the entire procedure takes only 0 minutes.. High Purity of DNA. OD 60 /OD 80 of plasmid DNA purified by this kit is >.8. Purified plasmid DNA can be used for many downstream applications such as DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic. No phenol/chloroform extraction and ethanol precipitation are required. Transportation: At ambient temperature. Lysis-Buffer-UF Buffer DW Universal Wash Solution II Elution Buffer EZ-0 96-Well Plate Deep Well Collection Plate 96 Storage Plate Sealing film B8008(BS908) 96 Well Plates 00 ml 00 ml 48 ml 0 ml 4 8 $3.5 B8008(BS909) 5 96 Well Plates X50 ml X0 ml X48 ml 50 ml 5 0 5 0 $6.50 66 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit This kit provides a simple, efficient and high throughput method for plasmid DNA purification. Plasmid DNA and DNA fragments are selectively adsorbed onto the EZ-0 columns of the 96-well plate. Other impurities such as proteins, salts and nucleotides are washed away. Plasmid DNA can then be eluted in a small volume of elution buffer. Purified plasmid DNA can be used for many downstream applications, including PCR, DNA sequencing, enzyme digestion and transformation. Note: the diameter of each colony should be > mm.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes 0 minutes.. Direct amplification from colonies, or glycerol stock solutions, no overnight culture is required. 3. Suitable for fast and large number screening. 4. Compatible with all downstream applications including PCR, restriction analysis and DNA sequencing. 5. Economic. Transportation: at ambient room temperature PT9043 96 Well Plates PT9044 5 96 Well Plates puc plasmid DNA purified using the 96-well single colony plasmid DNA MiniPrep Kit. EZ-0 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit This kit provides a simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Greater than 90% of the endotoxin can be removed using our Endotoxin-Be-Gone reagent. If large scale preparation is needed, EZ-500 Column can be used (B549(SK49) & B549(SK50)). Purified endotoxin-free plasmid DNA is suitable for transfection and other downstream applications.. Fast and convenient. Endotoxin is removed at the bacteria stage and the remaining steps are regular plasmid DNA extraction steps.. Efficient. A single treatment removes 90% of the endotoxin. Repeat procedure for greater efficiency. Purified plasmid DNA can be used for many downstream applications including transfection. 3. Economic. Solution I Solution II Solution III Wash Solution Elution Buffer Liquid Endotoxin Eliminator EZ-0 Column RNase A Solution (0mg/ml).0ml Collection Tube B698(BS798) 6 ml ml 5 ml 0 ml 5 ml 5 ml 50 0 μl 50 $0.90 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 67

EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit This kit provides a large-scale, simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Up to 500ug of endotoxin-free plasmid DNA may be obtained per maxi prep. Greater than 90% of the endotoxin can be removed using bacterial endotoxin erasol at bacteria stage. Purified endotoxin-free plasmid DNA can be used for transfection and other downstream applications.. Fast. Using a maxi-prep spin-column format, the entire procedure takes only 40 minutes.. High Quality of DNA. >90-99% of endotoxin can be removed. 3. Economic. SK49 SK50 4 Maxi-Preps 0 Maxi-Preps MAXI Resuspension Solution 55 ml x0 ml MAXI Cell Lysis Solution 55 ml x0 ml MAXI 3 Neutralization Solution 55 ml x0 ml MAXI 4 Equilibration Solution 54 ml x35 ml MAXI 5 Washing Solution 4x55 ml 4X75 ml MAXI 6 Elution Solution 54 ml x35 μl Liquid Endotoxin Eliminator 8 ml 40 ml RNase A (50 mg / ml) 88 μl x0 μl MAXI Column 4 0 $86.0 $344.40 EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit The kit provides a simple and efficient Spin Column approach for Mini plasmid DNA purification from yeast. Plasmid DNA of lysed yeast cells is selectively bound onto the column. Other impurities such as proteins and salts do not bind to the column and are eliminated in the flow through. Purified plasmid DNA can be used for PCR, transformation, restriction digestion and many other downstream applications.μg of plasmid DNA can be purified from 5 ml yeast cells.. Fast. Using a rapid spin-column format, the entire procedure takes approximately hours.. High Quality of DNA. OD 60 /OD 80 of purified DNA is generally >.8. 3. Compatible with most downstream applications such as PCR, restriction digestion and hybridization. 4. Non-Toxic. Phenol/chloroform extractions or ethanol precipitation is not required. 5. Direct manufacturer with most competitive price advantage. Transportation: at ambient temperature Buffer P Buffer P Buffer P3 Buffer DW Universal Wash Buffer Elution Buffer B60467(BS467) 0 Preps 3 ml 3 ml 4 ml 5 ml 3 ml ml B60467(BS468) 5 ml 5 ml 0 ml 5 ml ml 5 ml RNase A Solution (0mg/ml) 5 μ 0 Snailase Snailase Storage Buffer Snailase Reaction Buffer EZ-0 Spin Column.0ml Collection Tube 60 mg 0.5 ml 7 ml 0 0 $.00 B60467(BS469) 75 ml 75 ml 00 ml 5 ml 60 ml 5 ml 60 μl 500 mg.5 ml 75 ml 50 50 $ 336.00 l μl 0 300 mg.5 ml 35 ml 50 50 $84.00 68 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Genomic DNA Purification & Extraction Kits EZ-DNA Reagent EZ-DNA Reagent is a ready-to-use reagent for the isolation of genomic DNA from various samples, including animal tissue, cultured cells, and bacteria. The method is based on the use of an optimized guanidine-detergent solution, which lysis the tissue quickly and selective precipitates DNA from a cell lysate. During the isolation step, tissue or cells are lysed in EZ-DNA Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, purified DNA is dissolved in water, TE buffer or 8 mm NaOH. The protocol allows for fast isolation of genomic DNA from a large number of samples of small or large volume. The whole procedure takes about 0-30 minutes with DNA recovery of 70-90%. There is no need for overnight digestion, phenol extraction. Isolated DNA can be used directly for most applications such as Southern analysis, molecular cloning and PCR.. Fast. The entire procedure takes only 0-30 minutes.. Simple. One-Tube procedure, no phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. Economic. Transportation: tambient temperature. EZ-DNA Reagent A8080(BS80) 00 preps 00 ml $5.50 DNA extraction from various species using EZ-DNA Reagent. M. DNA marker. Lane -3: Genomic DNA from Bacteria. Lane 4-6: Genomic DNA from Rat. Lane 7-9: Genomic DNA from Yeast. One-Tube Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative and Gram-positive bacteria. Purified DNA can be used for most downstream applications, including PCR, restriction digestion, and hybridization.. Fast. Te entire procedure takes less than 0 minutes.. Simple. No phenol/chloroform extraction or ethanol precipitation is required. 3. Economic. Transportation: At ambient temperature. B80843(BS843) 00 Preps B80843(BS844) 500 Preps $ 39.90 $59.60 DNA isolated from various bacteria. M: DNA marker. Lane -3: Escherichia coli (Gram-Negative). Lane 4-6: Staphyloccocus aureus (Gram-Positive). Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 69

Rapid Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative or Gram-positive bacteria. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, and hybridization.. Fast. It takes less than 30 minutes.. Simple. 3. Economic. Transportation: Room temperature Universal Digestion Buffer Buffer PB TE Buffer B585(BS85) 5 ml ml 0 ml $36.75 EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit This kit provides a simple and convenient silica membrane-based technique to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. Total hands-on preparation time is less than 30 minutes. Bacteria samples are lysed in an optimized buffer. Released DNA binds specifically to the column while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps. Pure DNA is then eluted in either water or a low-salt buffer provided with the kit. The kit is also suitable for isolation of bacterial genomic DNA from colonies on a dish. Purified genomic DNA is 0-50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications.. Fast. Using a rapid spin-column format, the entire procedure takes approx 0 minutes.. High yield. 5-0 μg of bacterial genomic DNA can be obtained from ml (0 8-0 9 cells) of overnight culture. 3. High quality of DNA. OD 60/80 ratio of purified DNA is generally >.8. 4. Non-Toxic. No phenol extraction or ethanol precipitation is required. 5. Economic Transportation: At ambient temperature. B6043(BS43) B80064(BS64) EZ-0 Spin Column 50 50.0ml Collection Tube 50 50 $44.0 $83.75 DNA isolated from various bacteria. M: DNA Marker Lane -3: Escherichia coli (Gram Negative) Lane 4-6: Staphyloccocus aureus (Gram Positive) 70 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue 96-Well Plate Bacterial Genomic DNA Mini-Preps Kit The kit provides a simple and convenient high throughput approach to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. DNA in a cell lysate is selectively adsorbed on to the EZ-0 column while impurities such as proteins, salts and nucleotides are washed away. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be up to 50 kb in length. Genomic DNA purified can be used for PCR, and other downstream applications.. Suitable for Gram-positive and Gram-negative bacteria.. Fast. Using a high throughput 96-well plate format. 3. High purity of DNA. A 60/80 ratio of purified DNA is generally >.8. 4. Compatible with downstream applications. Transportation: At ambient temperature. Elution Buffer 96-Well Plate Deep Well Collection Plate 96-Well Storage Plate Sealing film B809(SK9) 96 Well Plates 4 8 $59.60 B8095(SK95) 5 96 Well Plates 5 0 5 0 $365.40 E. coli genomic DNA purified using the 96-well plate mini-preps kit. Shown on 0.8% agarose with DNA ladders. EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit This kit provides a simple and convenient technique for large-scale isolation of high quality DNA from both Gram-negative and Gram-positive bacteria. Up to 500 μg of DNA is selectively bound to EZ-500 spin column. Other impurities present in the lysate, including proteins and salts, do not bind on the column and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. The kit is also suitable for the isolation of bacterial genomic DNA from colonies grown on a dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. For isolation of DNA from Gram positive bacteria, bacteria should be pre-treated with a buffer containing lysozyme.. Suitable for both Gram-positive and Gram-negative bacteria.. High purity of DNA. Purified DNA has an A 60/80 ratio of between.6 and.9 3. Compatible with many downstream applications. 4. Direct manufacturer with most competitive price advantage. Universal Digestion Buffer Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer ( ph 9.0) Proteinase K (0mg/ml) Maxi Columns 50 ml Collection Tube B80038(PT9038) Maxi Preps 40 ml 50 ml 8 ml 7.5 ml 5 ml 5 ml 4 4 $86.0 3 4 5 6 7 M E.coli genomic DNA purified using the EZ-500 Spin Column Bacteria DNAMaxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker -7: genomic DNA from E.coli Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 7

Rapid Yeast Genomic DNA Extraction Kit This kit is designed for rapid, small-scale extraction of high quality genomic DNA from yeast. Yeast cell walls are digested by Snailase (or lyticase, zymolyase) and the membrane is lysed in the presence of proprietary buffers. DNA is then precipitated and washed with alcohol. Purified DNA can be used for many downstream applications, including PCR, restriction digestion, and hybridization.. Fast. The entire procedure takes less than 40 minutes.. Easy to use. 3. Economic. Universal Digestion Buffer Buffer PY TE Buffer Snailase Reaction Buffer Snailase Storage Buffer Snailase B587(BS87) 5 ml ml 0 ml 75 ml 5 ml 600 mg $36.75 Genomic DNA isolated from Saccharomyces cerevisiae Lane -3: Genomic DNA from Saccharomyces cerevisiae. EZ-0 Spin Column Yeast Genomic DNA Mini-Preps Kit The kit provides a simple and convenient method for the isolation of high quality yeast DNA. DNA present in cell lysate is selectively bound to the spin column while other impurities such as proteins and salts do not bind and are eliminated in the flow through. Purified genomic DNA is 0-50 kb in length, and is suitable for many downstream applications, including restriction endonuclease digestion, PCR, and cloning.. Fast. Using a rapid spin-column format, the entire procedure takes approx 30 minutes.. High Quality of DNA: OD 60 /OD 80 of purified DNA is generally.7-.9. 3. Non-Toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: At ambient temperature including Proteinase K and Snailase. Universal Digestion Buffer Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer ( ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase B80857(BS857) ml 6 ml ml 8 ml 7.5 ml 5 ml. ml 75 ml 5 ml EZ-0 Spin Column 50.0ml Collection Tube 50 600 mg $60.90 Genomic DNA isolated from Saccharomycetaceae 7 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue 96-Well Plate Yeast Genomic DNA Mini-Preps Kit This kit provides a simple and convenient, high-throughput approach to isolate high quality DNA from yeast. DNA present in cell lysate is selectively adsorbed onto the EZ-0 column, while impurities such as proteins, salts and nucleotides are washed away. Purified genomic DNA can be up to 50 kb in length, and can be used for a wide array of downstream applications, including PCR and restriction endonuclease digestions.. Fast. using the rapid spin-column and high throughput format, the entire procedure takes only hours.. High quality of DNA. OD 60/80 of purified DNA is generally.7-.9. 3. Non-toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: at ambient temperature (including Proteinase K and Snailase). Universal Buffer Digestion Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer (ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase 96-Well EZ Plate 96-Well Collection Plate 6 96-Well Storage Plate Sealing film B808357(BS8357) 96 Well Plates 48 ml 4 ml 48 ml 7 ml 30 ml 5 ml 4.8 ml 300 ml 0 ml.4 g 8 $86.90 Saccharomyces cerevisiae genomic DNA purified using the 96-well plate Yeast Genomic DNA Mini-Prep Extraction Kit. Rapid Fungal Genomic DNA Isolation Kit This kit provides a simple and efficient method for the isolation of total DNA (genomic and mitochondrial) from fungal species. The size of genomic DNA purified by this procedure is around 0-50 kb. Purified DNA can be readily used in a number of downstream applications including RT-PCR, SNP analysis, and restriction digestion.. Fast. the entire procedure takes less than 40 minutes.. Easy. 3. Economic. Universal Digestion Buffer Universal Buffer PF TE Buffer B8045(FT745) 50 preps 5 ml ml 0 ml $36.75 DNA isolated from various fungal species M: Marker Lane -: Penicillium spp. Lane 3-4: Pleurotus ostreatus. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 73

EZ-0 Spin Column Fungal Genomic DNA Mini-Preps Kit This kit provides a simple and convenient method for the isolation of high quality DNA from various fungal species. DNA present in a cell lysate is selectively bound to the spin column, while other impurities such as proteins, salts, and nucelotides do not bind and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. Purified genomic DNA is 0-50 kb in length, and is suitable for downstream applications, including restriction digestions, cloning, and PCR.. Fast and Simple. Using a spin-column format, the entire procedure takes only 30 minutes.. High quality of Fungal DNA. 3. Economic. B800(FT80) 50 preps Universal Digestion Buffer ml Universal Buffer PF 6 ml Universal Buffer BD ml Universal PW Solution 8 ml Universal Wash Solution 7.5 ml TE Buffer (ph 8.0) 0 ml Proteinase K. ml Genomic DNA isolated from various fungi EZ-0 Spin Column 50 Lane -3: Penicillium spp. genomic DNA -ml Collection Tube 50 $44.0 Lane 4-6: Pleurotus ostreatus. DNA EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit This kit provides a simple and efficient method for the isolation of genomic DNA from a variety of fungal species. DNA present in a cell lysate is selectively adsorbed in the column while other impurities are eliminated. Average DNA yield is ~0-00μg per preparation. Average size of the genomic DNA purified by this procedure is around 0-50 kb. No phenol/chloroform extraction, ethanol precipitation, or liquid nitrogen homogenization is required in this protocol. Purified fungal genomic DNA can be used for PCR, SNP, RAPD, RFLP, AFLP, Southern blotting, transformation and other applications.. Fast.. High Quality of DNA. OD 60/80 ratio of purified fungal genomic DNA is generally >.8. 3. Suitable for various filamentous fungal species including S. cerevisiae, S. pombe, Pichia pastoris, Candida albicans, Aspergillus niger, Collectotrichum musae, and Fusarium oxysporum. Universal Digestion Buffer Universal Buffer PF Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) Proteinase K EZ-500 Spin Columns 4 50-ml Collection Tube 4 B80037(PT9037) 4 Maxi Preps 50 ml 5 ml 50 ml 8 ml 7.5 ml 0 ml 5 ml $86.0 Purified fungal genomic DNA run on a 0.7% agarose gel. 74 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Rapid Plant Genomic DNA Isolation Kit This kit is designed for the rapid isolation of genomic DNA from plant leaves and seeds. Samples may be fresh, frozen, or dried. The whole procedure takes approximately 5 minutes and the kit is suitable for screening and high throughput extraction. No phenol/chloroform extraction or ethanol precipitation is required. Purified Genomic DNA can be used for RT-PCR, cloning and other downstream applications.. Fast. The entire procedure takes less than 30 minutes.. Easy. 3. Direct manufacturer with most competitive price advantage. B8086(PT786) 00 preps $5.50 B8086(PT787) 500 preps $0.00 High molecular genomic DNA isolated from various plant species using the Rapid Plant DNA Kit. Lane -: Genomic DNA from Nicotiana tabacum Lane 3-4: Genomic DNA from Arabidopsis thaliana Lane 5-6: Genomic DNA from Oryza sativa M: DNA marker. EZ-0 Spin Column Plant DNA Mini-Preps Kit This kit provides a simple and efficient method for the isolation of plant genomic DNA. Samples may be fresh, frozen, or dried. DNA in plant tissues & cells is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Average size of genomic DNA purified by this procedure is ~30-50 kb. Purified genomic DNA can be used directly in PCR, Southern Blotting, RAPD, RFLP, and many more.. Fast. Using a rapid spin-column format, the entire procedure takes less than 30 minutes.. High Purity of DNA. OD 60/80 of purified DNA is generally >.8. 3. Compatible with many downstream applications such as PCR, restriction digestions, real-time PCR, multiplex PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 4. Suitable for a wide variety of plant species and tissue types including some recalcitrant specimens. B80045(BS45) 50 preps μ B80045(BS66) 50 preps μl EZ-0 Spin Column 50 50 ml Collection Tube 50 50 $44.0 $83.75 Genomic DNA isolated from various plant species using the EZ-0 Plant DNA mini-prep kit. Lane -: Nicotiana tabacum Lane 3-4: Arabidopsis thaliana Lane 5-6: Oryza sativa Lane 7-8: Platycladus orientalis Lane 9-0: Wisteria sinensis M: DNA marker Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 75

EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit B80035(PT9035) 4 preps/ B80035(PT9036) 0 preps The kit is designed for the isolation of large quantities of genomic DNA from a wide variety of plant species. Samples may be fresh, frozen, or dried. DNA in plant tissues and cells are selectively adsorbed on the EZ-500 maxi-prep spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Up to 500 μg of plant DNA can be obtained per preparation. Average size of DNA purified using this procedure is ~30-50 kb. Purified genomic DNA can be used directly for PCR, Southern Blotting, RAPD, and more.. High Purity of DNA. OD 60/80 of purified DNA is generally >.8.. Compatible with many downstream applications, including such as PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 3. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Transportation: At ambient temperature. 96-Well Plate Plant Genomic DNA Mini-Preps Kit E.coli genomic DNA purified using the EZ-500 Spin Column Plant DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker -7: genomic DNA from E.coli 3 4 5 6 7 M This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from various plant species. DNA in a plant lysate is selectively adsorbed on each column of the plate while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can adsorb up to 0 μg of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified plant genomic DNA is 0-40 kb, and is suitable for an array of downstream applications including PCR, Southern blotting, microsatellite analysis, AFLP, RFLP, and RAPD.. Fast. Using a spin column format, the entire procedure takes less than hours.. High purity of DNA. OD 60/80 of purified DNA is generally >.8. 3. Compatible with many downstream applications. 4. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. B80836(BS836) 96 Well Plates Universal Buffer PCB 30 ml Universal Buffer BD 50 ml Universal PW Solution 7 ml Universal Wash Solution 30 ml TE Buffer (ph 8.0) 40 ml 96-Well EZ Plate 96-Well Collection Plate 6 96-Well Storage Plate Sealing film 8 $86.90 Plant genomic DNA purified using the 96-well Plate DNA Mini-Prep Kit. 76 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue EZ-0 Spin Column Soil DNA Mini-Preps Kit This kit provides PCR-inhibitor free DNA through a convenient and rapid method for the detection of microorganisms from soil, sand and fecal samples. These samples are considered challenging as they contain rich humic acid that can interfere with the PCR reaction. The kit removes all traces of humic acid using an optimized buffer during the spin column procedure. Briefly, the soil sample is lysed using lysis buffer (Buffer SCL). DNA is then bound to an EZ-0 column in the presence of chaotropic salt. Under these conditions only DNA will bind to the membrane while most of the contaminating RNA, proteins, and cell debris are removed in the flow through. Purified inhibitor-free DNA is then eluted into 50-00 μl of the provided TE buffer or water. Total genomic DNA can be isolated and purified from various microorganisms found in soil, including bacteria, fungi and algae. Purified humic acid-free DNA is of the highest quality and is fully compatible with PCR applications or other downstream applications. The molecular size of the purified DNA is around 0-50 kb. Average DNA yields are 5-0 μg per gram of soil sample.. Fast. Using a rapid spin column format, the entire procedure takes 30 minutes.. Versatile. All types of soil samples can be processed with this kit, including sand and fecal samples. 3. High Quality of DNA. OD 60 /OD 80 of purified DNA is generally.7-.9. The purified DNA is almost free from all inhibitors including humic acid. 4. Economical SCL Solution SP Solution SB Solution Wash Solution Elution Buffer Spin Column B8036(ST836) 50 preps 5 ml 5 ml 40 ml ml 5 ml 50 ml Collection Tube 50 $44.0 DNA purified from soil samples. One-Tube Tissue DNA Extraction Kit This kit facilitates rapid isolation of genomic DNA from a wide array of sample types, including animal tissue, mouse tail and ear clips, human hair or saliva, and fish and insect tissue. There is no need for phenol extraction, overnight digestion, DNA precipitation or column purification; the lysate can be used in PCR directly. One-tube extraction kit minimizes the cross-contamination between samples. The procedure takes less than 5 minutes, and the kit is suitable for high throughput PCR screening of large numbers of samples. For other applications, Rapid Animal DNA Isolation Kit and EZ-0 Spin Column Animal DNA Mini-preps Kit (codes: B80047(BS47) B804780(AT478); B804780(AT4780); BS48) are recommended. Samples recommended: 0.3-0.5 cm mouse tail; or 0.5- mm mouse ear punch; or -5 mg piece of tissue; or -0 hairs with roots; or 0 µl saliva; or -3 mm piece of zebrafish fin.. Fast. The entire procedure takes less than 5 minutes.. High Quality of DNA. OD 60 /OD 80 of purified DNA is generally.7-.9. 3. Versatile. Compatible with tissues from mice, fish and insects, human hair, saliva, swab, blood clot and so on. 4. Easy to scale up. 5. Economic. B5840(BS840) B5840(BS840) 00 Preps 500 Preps Proteinase K ml 0 ml $39.90 $59.60 PCR amplification using DNA isolated by One-Tube Tissue DNA Extraction Kit as template. Lane -3: template DNA from Rat muscle Lane 4-6: template DNA from Rat liver Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 77

Rapid Animal Genomic DNA Extraction Kit This kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of fresh or frozen animal cells and tissues. Purified DNA can be used for many downstream applications such as PCR restriction digestion, hybridization and other applications.. Rapid and simple. The entire procedure takes only 0 minutes.. High Quality of DNA. OD 60/80 ratio of purified DNA is generally >.8. 3. Non-Toxic. 4. Easy to scale up. 5. Economic. B804780(AT4780) B804780(AT478) B804780(AT478) 0 Preps $6.5 $05.00 $40.00 DNA isolated from various animal tissues Lane -: Rat muscle DNA Lane 3-4: mouse liver DNA Lane 5-6: fish DNA EZ-0 Spin Column Animal Genomic DNA Mini-Preps Kit The kit is designed for rapid small scale extraction of genomic DNA from various animal tissues (fresh or frozen). DNA from cell lysate is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids are eliminated. No phenol extraction or ethanol precipitation is required. Purified DNA can be used for many downstream applications including PCR, restriction digestion, and hybridization.. Fast. Using a rapid spin-column format, the entire procedure takes only 0 minutes.. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8. 3. Compatible with many downstream applications. 4. Economic. B80047(BS47) B80047(BS68) EZ-0 Spin Column 50 50 ml Collection Tube 50 50 $44.0 $83.75 Gel electrophoresis of an animal genomic DNA sample purified using the EZ-0 Spin Column Animal DNA Minipreps Kit. Lane is DNA marker. 78 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit This kit provides an efficient method for the purification of large quantities of animal genomic DNA. DNA in tissue lysates is selectively adsorbed on the EZ-500 spin column, while other impurities are unable to bind and eliminated in the flow-through. No phenol extraction or ethanol precipitation is required. Purified genomic DNA can be used directly in a wide array of downstream applications.. Fast. Uses a rapid spin-column format, the entire procedure takes 0 minutes only.. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8. 3. Suitable for large scale extraction of DNA from fresh or frozen animal tissues or cells. 4. Economic. Universal Buffer ACL Universal Buffer CL CW Solution CW Solution CE Buffer Proteinase K EZ-500 Column 4 50-ml Collection Tube 4 B80034(PT9034) 4 Maxi Preps 50 ml 60 ml 3 ml 9 ml 0 ml 6 ml $86.0 96-Well Plate Animal Genomic DNA Mini-Preps Kit Gel electrophoresis of an animal genomic DNA sample purified by using EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit. Lane is DNA marker. This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from animal tissues and cells. DNA in a cell lysate is selectively adsorbed in the column while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can retain up to 0 μg of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be used for PCR and many other downstream applications.. Fast. Using a high-throughput spin column format, the entire procedure takes only 5 minutes.. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8. 3. Suitable for extraction of DNA from fresh or frozen animal tissues or cells. B80437(BS437) 96 Well Plates ACL Solution 80 ml 00 ml PBS Solution 80 ml 00 ml AB Solution 80 ml 00 ml Wash Solution Elution Buffer Proteinase K EZ-0 96 Well Plate Deep Collection Plate 96 Storage Plate Sealing film 0 5 4 $86.90 B80437(BS437) 5 96 Well Plates 5 0 5 $47.90 Genomic DNA from various animal tissues purified using the 96-well plate mini-preps kit. Shown on a 0.8% agarose gel. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 79

One-Tube Clinical Sample DNA Extraction Kit This kit is designed for rapid isolation of genomic DNA from clinical samples including blood, blood clots, saliva, hair, swabs, and cell shedding. A simple and rapid lysis method is utilized, and there is no need for phenol extraction, overnight digestion, DNA precipitation or column purification. DNA can be used in PCR directly. The procedure takes less than 5 minutes to complete, and the kit is suitable for high throughput PCR screening of large-scale samples. Samples recommended: 5-0 µl blood; or 0 µl saliva; or -5 mg blood clot; or Swab. PCR amplification of DNA isolated using. Fast. The entire procedure takes less than 5 minutes. the One-Tube Clinical Sample DNA. Suitable for screening and high-throughput automation. Extraction Kit. 3. Easy to scale up. Lane -4: template DNA from human 4. Economical. anti-coagulated blood. (+) Positive Control (-) Negative Control BS8403 BS8404 00 Preps 500 Preps Lysis Buffer-L 0 ml 50 ml $39.90 $59.60 Rapid Blood Genomic DNA Extraction Kit This kit facilitates the simple and rapid isolation of high quality genomic DNA from fresh or frozen anti-coagulated blood. Whole blood of mammalian animals consists of non-nucleated cells (e.g. red blood cells, platelets) and nucleated cells (e.g. white blood cells). The former is lysed and removed by a Red Blood Cell Lysis buffer (Buffer TBP), while the latter (containing genomic DNA) is collected and lysed by Buffer Digestion in the presence of Proteinase K. DNA is released to the solution and can be isolated by ethanol precipitation. Up to 5 ml of blood can be used in each prep. Average DNA yields are 0-60 μg per ml of whole blood. Purified DNA can be used directly in a wide range of downstream applications.. Fast. The entire procedure takes 30 minutes.. Simple. 3. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8. 4. Easy to scale up. 5. Economical. Transportation: At ambient temperature. Buffer TBP Universal Buffer Digestion Buffer PR Proteinase K TE Buffer B80478(BT478) 40 ml 0 ml 4 ml. ml 50 ml $36.75 Genomic DNA purified from whole animal blood using the Rapid Blood Genomic DNA Extraction Kit. : Chicken Blood. : Rabbit Blood. 3: Pig Blood. M: lambda DNA. Shown on 0.8% agarose 80 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue EZ-0 Spin Column Blood Genomic DNA Mini-Preps Kit This kit provides a simple, rapid and efficient technique for isolation of high molecular weight genomic DNA from fresh, frozen and anti-coagulated whole blood. DNA from cell lysate is selectively adsorbed on the column while other impurities, including proteins and lipids, are unable to bind and are washed away. Purified genomic DNA can be used in a number of downstream applications, including PCR, Southern blotting and SNP genotyping.. High quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8.. Fast. Using a rapid spin-column format, the entire procedure takes 0 minutes. 3. Non-Toxic. 4. Easy. No red blood cell lysis step required. 5. Economical. TBP Buffer B80483(BS483) EZ-0 Spin Column 50 50.0ml Collection Tube 50 50 $44.0 B80483(BS684) 50 preps $83.75 96-Well Plate Blood Genomic DNA Mini-Prep Kit Genomic DNA preparations from a single blood sample of human whole blood using the Kit. This kit provides a simple, rapid and high-throughput method for the isolation of high quality genomic DNA from blood. DNA in a lysate is selectively adsorbed on each column in the plate while other impurities such as proteins, salts, and lipids are washed away. Purified genomic DNA can be used for PCR, Southern blot analysis and other applications. Average yield is about 0-60 μg per ml of mammalian whole blood sample.. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8... Non-Toxic. No phenol extraction and no ethanol precipitation are required. 3. Easy. No red blood cell lysis step require 4. Economical. B8003(BT903) 96 Well Plates B8003(BT903) 5 96 Well Plates CE Buffer 40 ml 0 ml Proteinase K 4.8 ml ml EZ-0 96 Well Plate 5 Deep Well Collection Plate 96 Storage Plate 4 0 5 Genomic DNA purified from human blood using the 96-well plate Blood Genomic DNA Mini-preps Kit. Sealing film 8 0 $59.60 $365.40 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 8

One-Tube Viral DNA Isolation Buffer The One Tube Viral DNA Isolation Buffer is used for isolation of viral DNA from a broad range of cell-free clinical samples including serum, urine and plasma. The one-tube procedure is simple and rapid; no transfer between tubes is necessary, which helps in minimizing cross-contamination. Purified viral DNA can be used for PCR, Real Time PCR, and many other downstream applications.. High yield. The recovery yield is generally >85%.. Simple. 3. Compatible with many downstream applications including PCR and Real Time PCR. 4. Easy to scale up. 5. Economic. B58409(VT4785) B58409(VT4786) 50 preps 00 preps $5.50 $0.00 Viral DNA was extracted from 0. ml blood plasma with HBV titer of 000 copies/ml and dissolved in 00 μl water. A 5μl aliquot was used in realtime PCR. Red and green line represent our product; the Blue line represents a product from a competitor. EZ-0 Spin Column Viral DNA Mini-Preps Kit This kit provides a simple, rapid and highly reproducible method for the isolation of viral DNA from a broad range of cell-free clinical samples, including serum, urine and plasma. Viral DNA in lysate is selectively adsorbed in the spin column while impurities are unable to bind and are removed in the flow through. PCR inhibitors, proteins and salts are completely removed during wash steps. Purified nucleic acids are then eluted in either water or TE buffer. The procedure is simple and fast. Purified viral DNA can be used for PCR, Real Time PCR and other clinical research applications.. Fast and Simple. Using a rapid spin-column format the entire procedure takes only 0 minutes.. Sensitive. 30-50 virus copies in ml of sample can be detected by PCR. 3. Non-Toxic. No phenol/chloroform or ethanol precipitation is required. 4. Versatile. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications. Suitable for broad range cell-free clinical samples including serum, urine and plasma. Lysis-Buffer-V Universal Wash Solution TE Buffer Spin Column ml Collection Tube B5867(VT88) 50 preps 30 ml 5 ml 0 ml 50 50 $ 7.40 B5867(VT883) 50 preps 50 ml 75 ml 50 ml 50 50 $ 357.00 8 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue 96-Well Plate Viral DNA Mini-Preps Kit This kit provides a fast, simple and high-throughput technique for the isolation of viral DNA from a broad range of cell-free clinical samples, including serum, urine and plasma. Viral DNA in lysates is selectively adsorbed in each spin column of the 96-well plate, while other impurities are unable to bind and are washed away. The procedure is simple and rapid. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications.. Fast and Simple. Using a rapid spin-column & 96-well high throughput format, the entire procedure takes only 30 minutes.. Sensitive. 30-50 virus copies in ml of sample in each well can be detected by PCR. 3. Non-Toxic. No phenol/ chloroform and ethanol are required. 4. Compatible. Purified viral DNA can be used for PCR, Real Time PCR and other clinical applications. 5. Versatile. Suitable for broad range cell-free clinical samples including serum, urine and plasma. 6. Economical. Lysis-Buffer-V Universal Wash Buffer Universal Elution Buffer EZ-0 96 Well Plate Deep Well Collection Plate 96 Storage Plate Sealing film One-Tube Swab DNA Extraction Kit B8003(VT903) 96 Well Plates 0 ml 60 ml 0 ml 4 8 $43.60 This kit is designed for one-tube isolation of PCR-ready genomic DNA from a variety of swab materials, including buccal swab and virginal swab. There is no need for phenol extraction, overnight digestion, DNA precipitation or column purification, and the lysate can be use as a PCR template directly. The one-tube manipulation minimizes the cross-contamination between specimens. The kit is also suitable for DNA extraction from many other clinical samples such as blood, dried blood and sperm. Purified swab DNA can be used for PCR.. Fast and Simple.. Suitable for extraction of DNA from a wide range of clinical samples. 3. Non-Toxic. 4. Economical. B58405(T76) 0 Preps B58405(T763) 00 Preps B58405(T764) 500 Preps Lysis-Buffer-S Proteinase K Universal Buffer NST 4 ml 0.4 ml 4 ml $3.0 0 ml ml 0 ml $68.5 00 ml 0 ml 00 ml $73.00 One-Tube Hair DNA Extraction Kit This kit provides a simple and rapid method for the isolation of genomic DNA from hair and other forensic samples, including saliva, nails and dried blood. The kit includes Proteinase K and other reagents to break up most tissue samples as well as to separate proteins and other components from the DNA. No phenol extraction, overnight digestion, DNA precipitation or column purification. Lysate can be used directly as a PCR template. The onetube manipulation minimizes cross-contamination between specimens.. Fast and Simple.. Suitable for extraction of DNA from a wide range of clinical samples, including buccal swab, virginal swab, blood, dried blood and sperm. 3. Non-Toxic. 4. Economic. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 83

Lysis-Buffer-H Proteinase K ONE-4-ALL Genomic DNA Mini-Preps Kit B58407(BS8407).6ml 60μl $39.90 The kit is designed for rapid small scale extraction of genomic DNA from various sources of starting materials. DNA from cell lysate is selectively absorbed on the Spin Column, and other impurities such as proteins, salts are eliminated. No phenol extraction, no ethanol precipitations are required. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, hybridization and other applications. Fast. Using a rapid spin-column format, the entire procedure takes 0 minutes. High Quality of DNA. OD 60 /OD 80 ratio of purified DNA is generally >.8. Flexible and versatile. Isolation of genomic DNA from various sources. Transportation: At ambient temperature. B68503(BS88503) B68503(BS88505) CW Solution 9 ml 45 ml EZ-0 Spin Column 50 50 ml Collection Tube 50 50 $9.40 $369.60 Gel electrophoresis of an animal genomic DNA sample purified by using ONE-4-ALL Genomic DNA Minipreps Kit. Lane is DNA marker. All-In-One DNA/RNA Mini-Preps Kit All-In-One DNA/RNA kit is designed for simultaneous extraction of total RNA and genomic DNA from a single biological sample. DNA and RNA are isolated without splitting the sample prior to extraction. DNA and RNA can be isolated from cultured eukaryotic cells, animal and plant tissues.. Efficient. Genomic DNA and RNA can be simultaneously isolated in hr.. Simple. No phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. High yield and reproducibility. Transportation: At ambient temperature. Buffer Lysis-DR CW Solution CW Solution CE Buffer GT Solution NT Solution RNase Free Water EZ-0 DNA Column RZ-0 RNA Column B6803(BS8803) 50 preps 45 ml 3 ml 9 ml 0 ml 8 ml 6 ml 5 ml 50 50 $99.75 84 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue DNA/RNA/Protein Extraction Kits All-In-One DNA/RNA/Protein Mini-Preps Kit All-In-One DNA/RNA/Protein kit is designed for simultaneous extraction of total RNA, genomic DNA and protein from a single biological sample. DNA, RNA and protein are isolated without splitting the sample prior to extraction. DNA, RNA and protein can be isolated from cultured eukaryotic cells, animal and plant tissues. Efficient. Genomic DNA, RNA and proteins can be simultaneously isolated in hr. Simple. No phenol/chloroform extraction is required. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. Easy to scale up. High yield and reproducibility. Buffer Lysis-DRP CW Solution CW Solution CE Buffer GT Solution NT Solution RNase Free Water PP Solution PD Solution B68003(BS88003) 50 preps 45 ml 3 ml 9 ml 0 ml 8 ml 6 ml 5 ml 35 ml 0 ml EZ-0 DNA Column 50 RZ-0 RNA Column 50 $0.5 RNA Extraction & Purification Kits EZ-RNA Reagents EZ-RNA Reagents are used for the isolation of total RNA from cells and tissues. During sample homogenization or lysis, the reagents maintain the integrity of the RNA. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. RNA is then recovered by precipitation with ethanol or isopropyl alcohol. EZ-RNA Reagents perform well with both small and large quantities of tissue and cells, and are suitable for human, animal, plant or bacterial samples. RNA species of a wide MW range are isolated. The isolated RNA typically has an OD 60 /OD 80 ratio of.8-.0 when diluted into RNase-free water at ph 8.0, and can be used in most downstream applications.. High recovery yield.. Simple and rapid. The entire procedure takes only 0 minutes. 3. High Purity of RNA. 4. Compatible with many downstream applications, including RT-PCR, Northern Blot, and cdna synthesis. 5. Versatile. Suitable for RNA extraction from most sources including bacteria, fungi, animals and some plants. 6. Easy to scale up. 7. Economic. 5 ml and 00 ml of EZ-RNA Reagents are sufficient for 50 and 00 mini-preps. B800409 (BS409A) B800409 (BS40A) 00 Preps One Step RNA Reagent 5 ml $6.5 00 ml $84.00 Total RNA isolation by EZ-RNA Reagents. M: DNA marker. Lane -: Nicotiana tabacum total RNA. Lane 3-4: Fish total RNA. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 85

EZ-0 Spin Column Total RNA Mini-preps Kit This kit is designed for the purification of total RNA from a wide range of sample types, including bacteria, fungi, animals and plants. This kit simplifies total RNA isolation by combining the stringency of guanidine isothiocyanate lysis with the speed and purity of silica-membrane based purification. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the EZ column. RNA binds to the silica membrane, while proteins and other contaminants are removed in the flow-through. Purified RNA is then eluted in RNase-free Water, and is ideal for use in most downstream applications, including Northern blotting, RT-PCR, Quantitative PCR, Poly A+ RNA selection and Array analysis. Fast. Using a rapid spin column format, the whole procedure takes less than 0 minutes.. High Quality of RNA. Purified RNA has an OD 60 /OD 80 ratio of.9-.0. 3. Versatile. Suitable for purification of total RNA from various samples such as bacteria, fungi, animals and some plants. 4. Economic. Buffer RLT Buffer RW Universal RPE Solution RNase-free water Spin Columns Collection Tubes B536(BS36) 5 ml 30 ml ml 5 ml 50 50 $ 60.90 Rapid Bacteria RNA Isolation Kit This kit is designed for the preparation of high quality total RNA from bacterial cells. 0μg total RNA can be purified from 5 x 0 7 bacterial cells using this kit. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. Fast. Using fast lysis buffer, the whole procedure takes less than 40 minutes.. High Quality of RNA. Purified RNA has an OD 60 /OD 80 ratio of.9-.0. 3. Easy to scale up. 4. Economical. Total bacterial RNA isolated using the Rapid RNA isolation Kit. Buffer Rlysis-B RNase-free Water B5865(BS865) 50 ml 5 ml $40.95 86 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue EZ-0 Spin Column Bacterial Total RNA Mini-Preps Kit This kit provides a simple spin column technique for preparation of high-purity intact total RNA. The reagent contains guanidine isothiocyanate and β- mercaptoethanol that can inactivate ribonucleases present in cell extracts. RNA is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted in he presence of RNase-Free Water. 5-5 μg total RNA can be purified from 5 mg animal tissue using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly(A)+ selection and in vitro translation.. Fast. Using rapid spin column format and fast lysis buffer the whole procedure takes less than 30 minutes.. High Quality of RNA. Purified RNA has an OD 60 /OD 80 ratio of.9-.0. 3. Economical. B60583(BS583) 0 preps B60583(BS584) 00 preps EZ-0 Column 0 00 50 -ml Collection Tube 0 00 50 B60583(BS784) 50 preps $ 36.75 $ 57.50 $35.00 96-Well Plate Bacterial Total RNA Mini-Preps Super Kit Fig.. Total RNA purified from E. coli using the EZ0 Column Bacteria Total RNA Mini-Prep Kit. M: Marker; Lane -: E. coli total RNA. This kit provides a simple and rapid method for the extraction of high quality total RNA from a wide range of biological samples, including Gramnegative and Gram-positive cultures. RNA in a bacterial lysate is selectively bound on each column of the 96-well plate while other impurities such as proteins and salts are washed away. The entire procedure takes approximately 5 minutes. No ethanol precipitation is required. Purified bacterial total RNA can be used for many downstream applications such as RT-PCR, Northern Blot, and cdna synthesis. The kit is also suitable for RNA extraction from animal, fungi and some of plants samples. The kit may not be suitable for RNA extraction from the plants which contain high levels of secondary metabolites, polyphenols and polysaccharides. Plant RNA Isolation Kit and EZ-0 Spin Column Plant RNA Isolation Kit are recommended (Codes B8049(PT49) and B634(BS834) ).. Fast. Using a rapid spin and 96-well high throughput format, the entire procedure takes less than 5 minutes only.. High Purity of RNA. Purified RNA has an OD 60 /OD 80 ratio of.8-.0. 3. Suitable for RNA extraction from both of Gram negative and positive bacteria. 4. Economic. B80585(BS585) 96 Well Plates B80585(BS585) 5 96 Well Plates Buffer Rlysis-BG Universal GT Solution Universal NT Solution RNase-free Water EZ-0 96-Well Plate 5 Deep Collection Plate 4 0 96-well Storage Plate Sealing Film 8 5 0 Reproducible yields of total RNA from a single sample using EZ-0 96-well Spin Column Plate RNA Minipreps Kit. The bands at two ends are RNA markers $6.50 $590.0 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 87

EZ-0 Spin Column RNA Cleanup & Concentration Kit. This kit is designed for the rapid purification and concentration of total RNA from in vitro transcription products or from various other materials. More than 80% of the RNA in a given sample can be recovered, and is ready for most downstream applications, such as RT-PCR, Northern Blotting and in vitro translation.. Fast. Using a rapid spin-column format, the entire procedure takes only 5 minutes!. High Purity of RNA. Purified RNA can directly used for RT-PCR, electrophoresis and labelling reaction, Northern Blot and cdna synthesis. 3. High recovery yield. 4. Economic. Buffer RLT Universal RPE Solution RNase-free Water EZ-0 Spin Column -ml Collection Tube 96-Well Plate RNA Cleanup & Concentration Kit. B635(BS935) 50 preps 50 ml ml 5 ml 50 50 $68.5 This kit is designed for the rapid purification and concentration of total RNA from in vitro transcription products or various other materials. More than 80% of the RNA can typically be recovered, and is ready for most downstream applications including RT-PCR, Northern Blotting and in vitro translation.. Fast. Using a rapid spin-column and 96-well high throughput format, the entire procedure takes 0 minutes.. High Yield: Up to 80% of the RNA will be recovered. 3. High Efficiency: Complete removal of contaminants 4. Economic. Buffer RLT Universal RPE Solution RNase-free Water 96-Well Plate ml Deep Well Collection Plate 96-Well Storage Plate Caps for Storage Plate Sealing film B805(BRC5) x 96 Well Plates 00 ml 48 ml 0 ml 4 4 8 $6.50 88 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

Rapid Fungal RNA Extraction Kit BBI 05-07 Catalogue This kit is designed for the preparation of high quality total RNA from a wide range of fungal species. Total RNA can be purified from fresh or frozen filamentous fungi samples using this kit. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation.. Simple and rapid. The entire procedure takes only 40 minutes.. High quality of RNA. OD 60 /OD 80 ratio of purified RNA is generally >.8. 3. Easy to scale up. 4. Economic. Transportation: at ambient temperature Buffer Rlysis-F RNase-free Water B8046(FT746) 50 preps 50 ml 5 ml $ 40.95 Total RNA purified from Pichia pastoris using the Rapid Fungal RNA Isolation Kit. EZ-0 Spin Column Fungal RNA Mini-Preps Kit This kit is designed for the preparation of high quality total RNA from a wide range of fungal species. Fungal samples are first lysed and homogenized using Buffer FS. RNA in the homogenate is then selectively adsorbed on to the spin column. Finally, the total RNA is eluted from the membrane by the addition of RNase-Free Water. 3-5 μg total RNA can be purified from 30 mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.. Fast. Using a rapid spin-column format, the entire procedure takes approximately 0 minutes.. High quality of RNA. OD 60/80 of purified RNA is generally >.8. 3. Intact RNA. RNA is purified with intergrity. 4. Economic. Transportation: at ambient temperature Buffer Rlysis-FG Universal GT Solution Universal NT Solution RNase-free Water B695(BS995) 50 preps 5 ml 8 ml 6 ml 5 ml EZ-0 Spin Column 50 ml Collection Tube 50 $ 78.75 Total RNA isolated from filamentous fungi using the Fungal RNA Mini-Preps Kit. Lane -3: Fungal total RNA. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 89

Rapid Plant RNA Isolation Kit This kit is designed for the preparation of high quality total RNA from a wide variety of plant species and tissue types. Plant tissue is first lysed and homogenized using Buffer Rlysis-P. All contaminants, including polysaccharides and lipids, are removed by centrifugation. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. Fast. The whole procedure can be completed in 40 minutes. High Quality of RNA. The OD 60 /OD 80 of purified RNA is generally >.8 3. Versatile. Suitable for isolation of total RNA from a wide range of specimens such as Arabidopsis thaliana, tobacco, camphor and other samples. 4. Easy to scale up. 5. Economical. Transportation : at ambient temperature Buffer Rlysis-P Buffer PK RNase-free Water B8049(PT49) 50 preps 35 ml 4 ml 5 ml EZ-0 Spin Column Plant RNA Mini-Preps Kit $ 40.95 Total RNA from a plant leaf purified using the Plant RNA Extraction Plant polysaccharides and polyphenols have a tendency to form insoluble compounds with RNA, making the isolation of plant RNA a sometimesdifficult task. Our Total Plant RNA Purification Kit resolves this problem and can help you obtain the RNA samples you require for your research. RNA Purification using this kit is easy to operate, and the purified RNA sample is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.. Fast. Using a rapid spin-column format the entire procedure takes only 30 minutes.. Versatile. Suitable for the isolation of RNA from a wide range of specimens such as Arabidopsis thaliana, tobacco, camphor and other samples. 3. High Quality of RNA. The OD 60 /OD 80 of purified RNA is generally >.9. 4. Economic. Transportation: at ambient temperature Kit B634(BS834) 50 preps Buffer Rlysis-PG 5 ml Universal GT Solution 8 ml Universal NT Solution 6 ml RNase-free Water 5 ml EZ-0 Column 50 -ml Collection Tube 50 $78.75 Total RNA isolation from various plant species using the EZ0 Spin Column Plant RNA Mini-Prep Kit. Lane -: Arabidopsis thaliana. Lane 3-4: Nicotiana tabacum. Lane 5-6: Spinacia oleracea. Lane 7-8: Oryza sativa. Lane 9-0: Cupressaceae. Lane -: Cinnamomum camphora. 90 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

Rapid Viral RNA Extraction Kit BBI 05-07 Catalogue This kit is designed for the preparation of high quality viral RNA from cell-free liquid specimens, including blood serum, urine, body fluids. This method is fast and easy to perform, and no mechanical disruption or column purification is required. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.. High recovery yield. >90% of viral RNA can be recovered.. Simple and rapid procedure. Entire procedure takes less than 30 minutes and can be completed in one tube. 3. High Quality of viral RNA: Complete removal of contaminants. Isolation of total RNA for use in a large range of applications 4. Economic. Transportation: at ambient temperature Buffer VG RNase-free Water B58637(VT484) 50 preps 30 ml 5 ml $7.40 RNA isolated by Rapid Viral RNA Isolation Kit in Real-time PCR EZ-0 Spin Column Viral Total RNA Extraction Kit This kit simplifies the isolation of viral RNA from cell-free body fluids with a fast spin-column format. Viral RNA binds specifically to the silica membrane while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two washing steps, leaving pure viral RNA to be eluted in RNase-free Water. Purified RNA is ready to use in RT-PCR, Northern blotting or other downstream applications.. Fast. Using a rapid spin column format, the entire procedure takes about 0 minutes.. High Yield. The recovery yield of viral RNA is generally >85% 3. Versatile. Suitable for purification of viral RNA from a wide range of specimens, including serum, plasma, cell culture media, and milk. 4. Non-toxic. No Phenol/chloroform extraction. 5. Economic. Transportation: at ambient temperature Buffer Rlysis-VG Universal RPE Solution RNase-free Water EZ-0 Spin Column -ml Collection Tube B58667(VT8) 50 preps 30 ml ml 5 ml 50 50 $06.05 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 9

Rapid Animal Total RNA Extraction Kit This kit is designed for the preparation of high quality total RNA from animal tissue samples, including skeletal muscle, heart and connective tissues. Animal tissue is lysed and homogenized by Buffer Rlysis-A. RNA is then recovered by precipitation with Buffer LD. 0 μg total RNA can be purified from 5 mg animal tissue using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation.. Simple and rapid. The entire procedure takes approximately 40 minutes.. High Purity of RNA. OD 60 /OD 80 ratio of purified RNA is generally.9-.0. 3. Compatible with many downstream applications, including RT-PCR, Northern Blotting, cdna synthesis, Poly (A) purification, nuclease protection and in vitro translation. 4. Easy to scale up. 5. Economic. Transportation: at ambient temperature Solution A B8048(AT48) 50 preps 50 ml $5.50 B8048(AT48) 50 preps 50 ml $0.00 EZ-0 Spin Column Animal Total RNA Mini-Preps Kit RNA isolated from various animal tissues using the Rapid Animal Total RNA Extraction Kit. Lane -: rat muscle RNA. Lane 3-4: mouse liver RNA. Lane 5-6: fish RNA. M: DNA marker The EZ0 Spin Column Animal Total RNA Purification Kit provides a simple, rapid method for preparation of high-purity total RNA. The reagent contains guanidine isothiocyanate and β-mercaptoethanol that inactivate the ribonucleases present in cell extracts. RNA in the whole homogenate is selectively adsorbed on the spin column (silica membrane) while other impurities are washed away. Total RNA is eluted from the membrane by the addition of RNase-free water.. Fast. Using a rapid spin-column format, the entire procedure takes approx 5 minutes.. High Purity of RNA. OD 60 /OD 80 ratio of purified RNA is generally >.9. 3. Compatible with downstream applications such as Northern Blots, cdna synthesis, RT-PCR and qrt-pcr. 4. High integrity. Buffer AG maintains the integrity of the RNA 5. Economic. Transportation: at ambient temperature B63(BS83) 50 preps B63(BS83) 50 preps Universal NT Solution RNase-free Water EZ-0 Spin Column -ml Collection Tube 6 ml 5 ml 50 50 $65.0 30 ml 5 ml 50 50 $60.40 Total RNA purified using the EZ-0 Column Animal Total RNA Mini-Prep Kit. M: Marker; Lane -3: Rat tissue total RNA. 9 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

Rapid Blood RNA Isolation Kit BBI 05-07 Catalogue This kit is designed for the preparation of high quality total RNA from anticoagulated blood. 0 μg total RNA can be purified from ml anticoagulated blood using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation.. Fast. The entire procedure can be completed in 40 minutes.. Simple. Red Blood Cell Lysis Buffer is not required. 3. Easy to scale up. 4. Economic. Transportation: at ambient temperature Buffer Rlysis-R Buffer NS-A % SDS Solution RNase-free Water B8048(BT48) 0 preps 3 ml 4.5 ml 0.5 ml 6 ml $44.0 B8048(BT483) 50 preps ml.5 ml.5 ml 30 ml $57.50 EZ-0 Spin Column Blood RNA Mini-Preps Kit B8048(BT484) 50 preps 60 ml 08 ml ml 50 ml $630.00 Total RNA was isolated from 0.75 ml rabbit whole blood using Rapid Blood RNA Isolation Kit and dissolved in 0 μl water. 0 μl was used in formaldehyde denaturing gel electrophoresis. The Blood total RNA Purification Kit provides a quick and simple method for preparation of high-purity intact total RNA. The reagent contains guanidine isothiocyanate and β-mercaptoethanol that inactivate the ribonucleases present in cell extracts. RNA in the whole homogenate is selectively adsorbed on the spin column while other impurities are washed away. Total RNA is eluted from the membrane in presence of RNase-free water. 5-5 μg total RNA can be purified from 00 μl anticoagulated blood using this kit. Purified RNA is ready for most downstream applications including RT- PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation.. Fast. Using rapid spin column format, the entire procedure takes 30 minute.. Simple. Red Blood Cell Lysis Buffer is not required. 3. High Quality of RNA. 4. Economic. Transportation: at ambient temperature Buffer Rlysis-R Buffer NS-A % SDS Solution Universal GT Solution Universal NT Solution RNase-free Water EZ-0 Column B633(BS833) 50 preps ml.5 ml.5 ml 8 ml 6 ml 30 ml 50 -ml Collection Tube 50 $ 78.75 Agarose gel electrophoresis of total RNA from rat anticoagulated blood. Lane -3: blood total RNA. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 93

mrna Purification Kit from Total RNA This kit provides a convenient and easy-to-follow method for purification of mrna from total RNA. Each prep can use up to 0 μg total RNA as starting material. mrna is selectively bound on oligo-dt cellulose, while 99% of the remaining RNA, including rrnas and trnas, do not bind. Purified mrna can be used for Norhtern blotting, RT-PCR, in vitro translation, differential display, cdna synthesis or library construction, among others.. Fast. The entire procedure takes only 30 minutes.. High Quality of mrna. OD 60 /OD 80 of purified mrna is generally >.8. 3. Compatible with many downstream applications. 4. Easy to scale up. Transportation: At ambient temperature X Binding Buffer X Binding Buffer Wash Solution RNA Elution oligo-dt cellulose LPA RNase-free Water B8098(MT998) 5 Preps 5 ml 40 ml 30 ml 0 ml 5 mg 00 μl ml $0.90 EZ-0 Spin Column Bacterial Total RNA Mini-Preps Kit The kit allows efficient purification of total RNA from various samples. Total RNA is easily purified from animal or human cells/tissues using a simple spin format. 5-5 μg total RNA can be purified from 5 mg animal tissue using this kit. Purified RNA is ready for most downstream applications suchas RT-PCR, Northern Blotting, Poly(A)+ selection and in vitro translation.. Fast. Using rapid spin column format and fast lysis buffer the whole procedure takes less than 30 minutes.. High Quality of RNA. Purified RNA has an OD 60 /OD 80 ratio of.9-.0. 3. Economic. Transportation: At ambient temperature. Buffer RLT B68583(BS88583) 50 preps 0 ml B68583(BS88586) 50 preps 00 ml Proteinase K 0.6 ml 3 ml EZ-0 Column 50 50 -ml Collection Tube 50 50 $ 78.85 Fig.. Total RNA purified from E. coli using the EZ0 Column Bacteria Total RNA Mini-Prep Kit. M: Marker; Lane -: E. coli total RNA. 94 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

RNAse Free DNAse Set BBI 05-07 Catalogue Buffer RDD is optimized for on-column DNase Digestion. The buffer is also well-suited for efficient DNAase digestion in solution. DNAse is supplied in liquid form. Store at -0 C for up to 9 months. No freeze-thaw. RNase-Free DNAse ( U/μ Buffer RDD RNase-free Water B6853(BS8853) 50 preps 3 ml ml $3.0 EZ-0 DNAway RNA Mini-Preps Kit This kit is designed to purify RNA from small amounts of animal cells or tissues. Samples are lysed and homogenized by Buffer Lysis-DR. Genomic DNA contamination is removed using a gdna Eliminator column. Purified RNA is ready for most downstream applications including RT-PCR, Northern Blotting, Poly(A)+ selection and in vitro translation.. Efficient. Remove DNA during RNA purification.. High Quality of RNA. Purified RNA has an OD 60 /OD 80 ratio of.9-.0. 3. Fast. Entire procedure can be completed in 30 minutes. Transportation: At ambient temperature. B6833(BS8833) 50 preps B6833(BS8836) 50 preps gdna Eliminator Column 50 50 EZ-0 Column 50 50 Gel Extraction Kit $ 0.5 $44.00 Fig.. Total RNA purified from E. coli using the EZ0 Column Bacteria Total RNA Mini-Prep Kit. M: Marker; Lane -: E. coli total RNA. Gel Extraction Kit is an excellent tool offering a rapid and economic method to extract DNA from an agarose gel. This technology is based on binding DNA to silica-based membrane, followed by subsequent wash steps and then elute pure ready-to-use DNA. The high quality of extracted DNA can be used directly for any critical downstream application. Specification: Sampling Recovery Volume of Eluate Handling Time Up to 00 mg 70 ~ 95 % 5 ~ 50 μl Within 5 min Agarose Gel Transportation: At ambient temperature. A8000 (9K-006-000s) 0 Preps A8000(9K-006-000) 00 Preps GEL Solubilization Solution 0 ml 00ml 00m l GEL Washing Solution* 3ml 30ml 60ml GEL 3 Elution Solution ml 0ml 0ml GEL Column 0 00 00 Collection Tube 0 00 00 $ 4.00 $ 343.00 * Add 7 ml / 70 ml / 40 ml of ethanol (96 ~ 00%) to GEL Washing Solution when first open. A8000(9K-006-000) 00 Preps Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 95

PCR Purification Kit Bio Basic Inc s PCR Purification Kit is an excellent tool offering a rapid and economic method to purify DNA samples from any enzymatic reactions. This technology is based on binding DNA to silica-based membrane, followed by subsequent wash steps and then elute pure ready-to-use DNA. The very high quality purified DNA can be used directly for any critical downstream application. Specification: Sampling Recover Volume of Eluate Handling Time Up to 00 μl of PCR / Enzymatic reaction 80 ~ 95 % 5 ~ 50 μl Within 5 min Transportation: At ambient temperature. PCR Dilution Solution* PCR Washing Solution** PCR 3 Elution Solution GEL Column A830003 (9K-006-0003s) 0 Preps 4ml ml ml 0 A830003(9K-006-0003) 00 Preps Collection Tube 0 00 00 40ml 0ml 0ml * Add.7 ml/7 ml /34 ml of ethanol (96 ~ 00%) to PCR Dilution Solution when first open. ** Add 8 ml/80 ml/60 ml of ethanol (96 ~ 00%) to PCR Washing Solution when first open. Plasmid DNA Miniprep Kit 00 A830003(9K-006-0004) 00 Preps Bio Basic Inc s Plasmid DNA Extraction Miniprep Kit is an excellent tool offering a speed and economic method to purify plasmid DNA from bacteria cultures. This technology is based on binding DNA to silica-based membranes in chaotropic salts and washing DNA with specially formulated solutions. Compared with other harmful and time-consuming procedures, such as phenol / chloroform extraction and ethanol precipitation, Feldan Plasmid DNA Extraction Miniprep Kit shortens the handling time to about 5 min. The high quality plasmid DNA can be used directly for any downstream applications. 80ml 40ml 0ml 00 $ 00.00 $ 300.00 Specification: Sampling Yield Handling Time ~5 ml overnight culture up to 0 µg for high-copy plasmids ~5 min Transportation: At ambient temperature. A80009(9K-006-0009s) 0 Preps A80009(9K-006-0009) 00 Preps A80009(9K-006-000) 00 Preps MINI Resuspension Solution MINI Cell Lysis Solution MINI 3 Neutralization Solution MINI 4 Washing Solution (concentrated)* MINI 5 Washing Solution (concentrated)** MINI 6 Elution Solution 3ml 3ml 4ml 3.5ml ml RNase A (50mg/ml) 6μ μl 0 μl MINI Column 0 Collection Tube 0 00 00 30ml 30ml 40ml 35ml 0ml * Add,5 ml/5 ml/30ml ethanol (96 ~ 00%) to MINI 4 Washing Solution before first use. ** Add 8 ml/80 ml/60ml ethanol (96 ~ 00%) to MINI 5 Washing Solution before first use. 00 60ml 60ml 80ml 70ml 40ml 00 $ 79.00 $ 57.00 96 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

Genomic DNA Prep Kit Blood BBI 05-07 Catalogue A80003(9K-006-003) (400 preps) / A80003(9K-006-008) (3300 preps) Note: If you are using mammalian cells from cell culture, please start with pelleted cells at step. It is possible to use up to 6x0 6 cells per prep.. Add 300 μl of whole blood (or bone marrow) to a.5 ml microcentrifuge tube containing 900 μl of RBC Lysis Solution. Incubate for 3min. at room temperature. (Note: If blood collection occured > hour ago, increase the incubation time to 0 min to complete cell lysis.. Centrifuge for 30 sec. at 3,000-6,000 xg. Remove the supernatant with a pipet for leaving behind the visible white cell pellet and about 0-0 μl of the residual liquid. 3. Vortex the tube vigorously for 0 sec. to resuspend the white cells in the residual liquid. (The white cells should be completely 4. Add 300 μl Cell Lysis Solution to the resuspend cells and pipet up and down to release the cells. 5. Add 00 μl of Protein Precipitation Solution to the cell lysate. 6. Vortex vigorously for 0 sec. to mix well. 7. Centrifuge at 3,000 ~6,000xg for min. The precipitated proteins should form a tight, dark brown pellet. (If the protein pellet is not tight, repeat Step 6, followed by incubation on ice for 5 min then repeat Step 7.) 8. Transfer the supernatant to a clean.5ml microcentrifuge tube containing 300μl of 00% Isopropanol (-propanol ). 9. Mix the sample thoroughly by gentle inversions. 0. Centrifuge at 3,000-6,000 xg for min. (White DNA pellet will be formed.). Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300 μl of 80% Ethanol and invert the tube several times to wash the DNA pellet.. Centrifuge at 3,000-6,000 xg for min. Carefully discard the ethanol and dry at room temperature for about 0~5 min. 3. Add 50 μl ~00 μl of DNA Hydration Solution. 4. Vortex 5 sec. at medium speed to mix. 5. Incubate the sample at 65 C for 0~30 min. 6. Store the DNA at 4 C. (For long time storage, store sample at -0 C or -80 C). Genomic DNA Prep Kit Bacteria A8004(9K-006-004) (400 preps) / A8004(9K-006-009) (3300 preps) Prepare 00% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the provided vials to obtain recommended enzyme concentration: Lysozyme (00 mg/ml) RNaseA (4 mg/ml ) and store those solutions at -0 C. Preheat a bath at 37 C and 65 C for incubations.. Transfer ml of cultured cell to a.5 ml microcentrifuge tube. In case cell density is low, ml of Cultured cells can be used.. Harvest the cells by centrifugation (3,000 ~6,000 xg, min.) and discard the supernatant. 3. Resuspend the cell pellet in 300 μl of Cell Resuspension Solution. 4. Add μl of Lysozyme (00 mg/ml ) and mix well by inverting. 5. Incubate the tube at 37 C for hour. 6. Centrifuge (3,000 ~ 6,000 xg for min.) and discard the supernatant. 7. Resuspend the pellet in 300 μl of Cell Lysis Solution. RNase Treatment 8. Add.5 μl of RNase A (4 mg/ml) and mix by inverting. 9. Incubate at 37 C for 5 ~6 min. and cool on ice for min. Protein Precipitation 0. Add 50~00 μl of Protein Precipitation Solution and vortex vigorously for 0 ~ 30 sec.. Centrifuge at 3,000 ~6,000 xg for 5 min.. Transfer the supernatant to a clean.5 ml micro tube containing 300 μl of 00% Isopropanol. 3. Mix the sample thoroughly by gentle inversions. 4. Centrifuge at 3,000 ~6,000 xg for min. (White DNA pellet will be formed.) 5. Discard the supernatant and drain tube briefly on a clean absorbent paper. Add 500 μl of (80% Ethanol) and invert the tube several times to wash the DNA pellet. 6. Centrifuge at 3,000 ~ 6,000 xg for min. Carefully discard the Ethanol. 7. Air dry at room temperature for 0 ~5 min. DNA Hydration 8. Add 0 ~00 μl of DNA Hydration Solution to the dried DNA pellet. 9. Hydrate the DNA by incubating at 65 C for hour. 0. Store the DNA at 4 C. (For long time storage, store at -0 C or -80 C). Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 97

Genomic DNA Prep Kit Plant A80005(9K-006-005) (400 preps) / A80005(9K-006-000) (3300 preps) Prepare Isopropanol and 80% Ethanol before using the Kit. Please follow instructions on the vial to prepare the provided RNase A solution (4mg/ml) and store at -0. Prepare a bath at 65 for incubation. Sample collection & Handling Fresh or frozen tissue must be finely ground with a mortar and pestle in liquid nitrogen before DNA isolation. Work quickly and keep tissue cold to minimize DNAase activity. Cell Lysis. Transfer the finely ground tissue (0~30 mg) to a.5 ml microcentrifuge tube.. Add 300 μl of Cell Lysis Solution to the tissue. 3. Incubate at 65 C for 60 min. mix invert the tube every 5~0 min. during the incubation. RNase Treatment 4. Add.5 μl of RNase A (4 mg/ml) to the cell lysate. 5. Mix the sample by inverting the tube 5 times and incubate at 37 C for 5~60 min. Protein Precipitation 6. Cool the sample to room temperature and add 00 μl of Protein Precipitation Solution to the cell lysate. 7. Mix the solution by vortex. 8. Centrifuge at 3,000-6,000 xg for 3 min. (The precipitant should form a tight, green pellet. If the pellet is not tight, repeat step 7, incubate on ice for 0 min. and then repeat Step 8.) DNA Precipitation 9. Transfer supernatant to a clean.5 ml microcentrifuge tube containing 300μl of 00% Isopropanol (-propanol). 0. Mix the sample thoroughly by gentle inversions.. Centrifuge at 3,000~6,000 xg for min (white DNA pellet will be formed).. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300 μl of 80% Ethanol and invert tube several times to wash the DNA Pellet. 3. Centrifuge at 3,000~6,000 xg for min. Carefully discard the ethanol. 4. Air dry at room temperature for 0~5 min. DNA Hydration 5. Add 50μl ~00μl of DNA Hydration Solution to the dried DNA pellet. 6. Hydrate the DNA by incubating sample at 65 C for hour. 7. Store DNA at 4 C (For long time storage, store at -0 C or -80 C). Genomic DNA Prep Kit Fungi A8006 (9K-006-006) (400 preps) / A8006 (9K-006-00) (3300 preps) Prepare 00% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the provided vials to prepare the RNase A solution (4mg/ml) and the proteinase K solution (0mg/ml) and store those vials at -0 C. Preheat a bath at 55 C for Proteinase K reaction incubation then at 65 C for RNase reaction incubation. Cell Lysis. Transfer ml of the cultured cell into a.5 ml microcentrifuge tube. Alternatively, use 50-00 mg of fungal tissue or -3 pieces of 0.5x cm of Cultured plate.. Harvest the cell by centrifuge (3,000~6,000 xg, min.) and discard supernatant. 3. Resuspend the cell pellet in 300 μl of Cell Resuspension Solution. 4. Add.5 μl of Proteinase K (0 mg/ml) and mix by inverting gently but thoroughly. 5. Incubate the tube at 55 C for hour. 6. Centrifuge (3,000~6,000 xg, min.), and discard the supernatant. 7. Resuspend the pellet in 300 μl of Cell Lysis Solution. Protein Precipitation 8. Add 00 μl of Protein Precipitation Solution and vortex vigorously for over 0 sec. 9. Centrifuge at 3,000~6,000 xg for 5 min. DNA Precipitation 0. Transfer the supernatant to a clean.5 ml microcentrifuge tube containing 300 μl 00% Isopropanol (-propanol).. Mix the sample by inverting gently but thoroughly.. Centrifuge at 3,000-6,000 xg for min. (white DNA pellet will be formed.) 3. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300 μl of 80% Ethanol and invert the tube several times to wash the DNA pellet. 4. Centrifuge at 3,000-6,000 xg for min. Carefully discard the Ethanol. 5. Air dry at room temperature for 0~5 min. DNA Hydration 6. Add 50 μl ~00 μl of DNA Hydration Solution to the dried DNA pellet. 7. Hydrate the DNA by incubating at 65 C for hour. 8. Add.5μl of RNase A (4 mg/ml) and incubate at 37 C for 60 min. 9. Store the DNA at 4 C. (For long time storage, store at -0 C or -80 C). 98 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Genomic DNA Prep Kit Animal Tissue A8006 (9K-006-006) (400 preps) / A8006 (9K-006-00) (3300 preps) Prepare 00% Isopropanol and 80% Ethanol before using kit. Please follow instruction on the vials to prepare 4mg/ml RNaseA solution and 0mg/ml Proteinase K solution, then store those solutions at -0 C. Preheat a bath at 55 C for Proteinase K reaction incubation then at 65 C for RNase reaction incubation. Cell Lysis. Transfer 5~0 mg of fresh tissue or frozen tissue to.5 ml microcentrifuge tube.. Add 300 μl of Cell Lysis Solution to the tissue 3. Add.5 μl of Proteinase K (0 mg/ml) to lysate and mix by inverting. Incubate at 55 C overnight or until the tissue has dissolved. Protein Precipitation 4. Cool the sample at room temperature and add 00 μl of Protein Precipitation Solution to the cell lysate. 5. Mix the solution well by vortex over 0 sec. 6. Centrifuge at 3,000~6,000 xg for 3 min. (The precipitated protein should form a tight pellet. If the pellet is not tight, repeat step 5, incubate on ice for 0 min. and then repeat Step 6.) DNA Precipitation 7. Transfer the supernatant to a clean.5 ml microcentrifuge tube containing 300 μl of 00% Isopropanol (-propanol). 8. Mix the sample thoroughly by gentle inversions. 9. Centrifuge at 3,000~6,000 xg for min. The DNA will be visible as a pellet. 0. Discard the supernatant and drain tube briefly on clean absorbent paper. Add 300 μl of 80% Ethanol and invert tube several times to wash the DNA Pellet.. Centrifuge at 3,000~6,000 xg for min. Carefully discard the ethanol.. Air dry at room temperature for 0~5 min. DNA Hydration 3. Add 50 μl ~00 μl of DNA Hydration Solution to the dried DNA pellet. 4. Hydrate the DNA by incubating sample at 65 C for hour. 5. Add.5 μl of RNase A (4 mg/ml) and incubate at 37 C for 30~60 min. 6. Store the DNA at 4 C (For long time storage, store at -0 C or -80 C). Genomic DNA Prep Kit Yeast A80007(9K-006-007) (400 preps) / A80007(9K-006-00) (3300 preps) Prepare 00% Isopropanol and 80% Ethanol before using kit. Follow instruction on the provided vials of RNase A and Lyticase vial to obtain RNase A (4 mg/ml) and Lyticase (.5 U/μl ) and store those vials at -0 C. Preheat a source at 65 C for RNase reaction incubation. Cell Lysis. Transfer ml of the cultured cell into a.5 ml microcentrifuge tube.. Harvest the cell by centrifuging (3,000~6,000 xg, min.) and discard supernatant. 3. Resuspend the cell pellet in 300 μl of Cell Resuspension Solution. 4. Add μl of Lyticase (.5 U/μl ) and mix by gentle inversions. 5. Incubate the tube at 37 C for 60 min. 6. Centrifuge (3,000~6,000g, min), and discard the supernatant. 7. Resuspend the pellet in 300 μl of Cell Lysis Solution. Protein Precipitation 8. Add 00 μl of Protein Precipitation Solution and vortex vigorously for over 0 sec. 9. Centrifuge at 3,000~6,000 xg for 5 min. DNA Precipitation 0. Transfer the supernatant to a clean.5 ml microcentrifuge tube containing 300 μl of 00% Isopropanol (-propanol).. Mix the sample thoroughly by gentle inversions.. Centrifuge at 3,000~6,000 xg for min. (White DNA pellet will be formed.) 3. Discard the supernatant and drain the tube briefly on clean absorbent paper. Add 300 μl of 80% Ethanol and invert the tube several times to wash the DNA pellet. 4. Centrifuge at 3,000~6,000 xg for min. Carefully discard the ethanol. 5. Air dry at room temperature for 0~5 min. DNA Hydration 6. Add 50 μl ~00 μl of DNA Hydration Solution to the dried DNA pellet. 7. Hydrate the DNA by incubating at 65 C for hour. 8. Add.5 μl of RNase A (4 mg/ml) and incubate at 37 C for 30 min. 9. Store the DNA at 4 C. (For long time storage, store at -0 C or -80 C). Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 99

Plasmid DNA Maxi Kit Bio Basic Inc s Plasmid DNA Extraction Maxiprep Kit is an excellent tool offering a rapid and economic method to purify plasmid DNA from bacteria cultures. This technology is based on alkaline lysis and purification by Anion-exchange chromatography. Compared with other harmful and timeconsuming procedures such as phenol / chloroform extraction and ethanol precipitation, Bio Basic s Plasmid DNA Extraction Kit shortens the handling time to about hours. The high quality plasmid DNA can be used directly for any downstream applications. Specification: Sampling Yield Handling Time 00~50 ml of bacteria culture for high copy plasmids up to 500 µg About hours 00~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. A8003 (9K-006-003s) Prep A8003(9K-006-003) 0 Preps A8003(9K-006-007) 5 Preps MAXI Resuspension Solution ml 0ml 3X0m l MAXI Cell Lysis Solution MAXI 3 Neutralization Solution MAXI 4 Equilibration Solution MAXI 5 Washing Solution ml ml 3.5ml 55ml 0ml 0ml 35ml X75ml 3X0ml 3X0ml 3X35ml 6X75ml MAXI 6 Elution Solution 3.5ml 35 μl 3X3 5μl RNase A (50 mg / ml) μl 0 μl 3X 0μl MAXI Column Collection Tube $ 89.00 $ 567.00. Solutions provided in this kit contain irritants, wear gloves and lab coat when handling.. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI Resuspension Solution bottle. Add 50 μl of MAXI Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI Resuspension Solution bottle. Store at 4 C. 3. Check MAXI Cell Lysis Solution before use. Warm MAXI Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI Cell Lysis Solution. 4. To avoid acidification of MAXI Cell Lysis Solution from CO in the air, close the bottle immediately after use. 0 00 30 30 Columns & 96 Well Plates Code Description Size B65005(SD5005) EZ-0 Column & collection tube (blue tube, clear ring, clear collection) 00 $ 4.5 B65005(SD5005-EMPTY) EZ-0 Empty Column without filter, without collection tube & O-ring 000 $ 5.50 B65008(SD5008) EZ0 RNA Column & collection tube(clear tube, clear ring, clear collection) 00 $ 6.5 B65006(SD5006) 96 well filter plate (960 μl each well) $ 37.80 B65006(SD5006-EMPTY) 96 well filter plate (no filter) $ 5.0 B65007(SD5007) 96 well DNA plate with membrane (960 μl each well) $ 89.00 F80896(BR58-96N) 96 WELL ML DEEP PLATE, NATURAL,EDGE FILLED, 0 PLATES/BAG PK $ 7.00 00 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA

BBI 05-07 Catalogue Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) 474 4493, (800) 33 74 Fax:(905) 474 5794 460 Bailey Avenue, Amherst, New York, 46 USA 0