evol XR World s First Digital Analytical Syringe

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evol XR World s First Digital Analytical Syringe ArabLab 2016 Andy Hall - Trajan Scientific and Medical Akram Hussain - Kinesis Ltd 21 March 2016

Trajan Scientific and Medical Support Services and Territory Operations Your Partner with a Global Support Network Trajan has a global workforce of over 300 people, with customer service and logistics hubs in Australia, Europe the US and Asia.

Manufacturing - Melbourne, Australia

Manufacturing - Melbourne, Australia Probe and Needle Manufacturing - Clean, modern working environment to ensure highest quality. Part of the team that manufactures them.

Trajan Manufacturing / R&D Expertise

evol XR Digital Analytical Syringe

Existing Liquid Handling Problems Air-cushion pipettes are convenient for dispensing but cannot be used for dispensing non-aqueous, viscous or hazardous liquids. Only positive displacement devices such as syringes are suitable for dispensing these types of liquids. Pipettes can only be calibrated for aqueous liquids. Difficult to calibrate manual syringes - gravimetric method - user dependant

Existing Liquid Handling Problems Piston seal Piston Cushion of air Liquid is aspirated into the tip by a cushion of air moved by the piston. This air-cushion behaves like a spring that effectively holds the aspirated volume of liquid in the tip. This is the same as aspirating liquid with a syringe when there is an air-bubble between the plunger and the liquid Pipette tip Elastic properties of the air cushion are effected by variations in: - air pressure, - air humidity, - sample density, - sample viscosity.

Solution?

evol XR Digital Analytical Syringe evol is the union of two precision devices a digitally controlled electronic drive and XCHANGE Analytical Syringes

evol XR Digital Analytical Syringe The integrated XCHANGE coupling allows evol Syringes to be quickly and easily changed. A magnetic connection is made between the plunger drive and the syringe plunger. When the XCHANGE coupling is pulled back the syringe can be removed and replaced.

evol XR Digital Analytical Syringe Five (5) XCHANGE evol Syringes cover a volume range from 200 nl to 1000 ul with a precision and accuracy better than 0.5 % (full stroke) when calibrated. A similar volume range using a pipette would require between 5 to 12 individual pipettes and would not deliver the same precision and accuracy.

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Precision results are close together

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Precision results are close together Accuracy average result is accurate

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Precision results are close together Accuracy average result is accurate evol Accurate and Precise

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Manual dispensing with Analytical Syringe User A User B User C Not Precise Not Accurate Precise Not Accurate Precise Not Accurate User B and C are both able to manually dispense liquid precisely but since they are not accurate they will get different results User D Not Precise Not Accurate

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Manual dispensing with un-calibrated evol User A User B Precise Precise An un-calibrated evol removes The operator-error from any dispensing procedure, for any liquid with an accuracy better than ±1.0 % at full scale User C User D Precise Precise Even with an uncalibrated evol User A, B, C and D are all able to manually dispense any liquid precisely

evol - Accuracy and Precision evol is both precise and accurate independent of the user s skills Manual dispensing with a calibrated evol User A User B User C User D Precise & Accurate Precise & Accurate Precise & Accurate Precise & Accurate A calibrated evol removes the mechanical-error from any dispensing procedure, for any liquid with an accuracy better than ±0.5 % at full scale With a calibrated evol User A, B, C and D are all able to manually dispense any liquid precisely and accurately

evol - User Calibration evol is the world s first truly calibrated analytical syringe To comply with your stringent laboratory standards (e.g. GLP, GMP, FDA) evol can be easily calibrated to ensure accurate dispensing at all times. Through a simple gravimetric calibration procedure a calibration factor is calculated that automatically adjusts the plunger drive to vastly improve syringe accuracy. Calibration factors can be stored for up to ten evol syringes and can be quickly loaded when the syringe is changed. 7

evol - User Calibration Calibration Worksheet Available at www.sge.com/evol Select syringe Enter calibration dispense volume Enter density for calibration liquid 7

evol - User Calibration Dispense x 10 500ml calibration liquid on analytical balance. Enter weight (mg) Worksheet converts dispensed weight to volume dispensed Average dispensed volume calculated. User enters this value in handle Calculated calibration factor 7

evol - Accuracy and Precision Syringe Accuracy Precision Calibrated Syringe at Full Scale Calibrated Syringe at 10 % Scale Uncalibrated Syringe at Full Scale RSD at Full Scale RSD at 10 % Scale 1 ml ± 0.2 % ± 0.5 % ± 0.5 % ± 0.3 % ± 0.6 % 500 µl ± 0.2 % ± 0.5 % ± 0.5 % ± 0.3 % ± 0.6 % 100 µl ± 0.2 % ± 0.5 % ± 0.7 % ± 0.4 % ± 0.7 % 50 µl ± 0.2 % ± 0.5 % ± 1.0 % ± 0.4 % ± 0.8 % 5 µl ± 0.2 % ± 1.0 % ± 1.0 % ± 0.5 % ± 1.0 %

evol - Applications The perfect solution for routine repeat dispensing of all liquids: especially Volatile solvents (e.g. organic solvents) Hazardous chemicals Corrosive chemicals Viscous samples These are liquids that cannot confidently be dispensed accurately or precisely with a pipette #1 A separate aspirate and dispense speed can be set with evol for volatile and viscous liquids #1 References to the incompatibility of pipettors for dispensing these liquids are commonly found on manufacturers web sites

evol - Applications evol will be valued for all liquid handling applications that require accurate dispensing. Accuracy is always important for quantitative tests that rely on absolute readings or comparisons to external standards. Examples: - Preparation of calibration standards (internal and external) - Dispensing of internal standards - Preparation of control samples - Serial dilutions - Sample spiking (surrogate samples) - Antibody addition in immunoassays - Impurities and so on.

evol - Target Applications The perfect solution for routine liquid handling applications in: Clinical and Forensic Toxicology Laboratories (preparation of calibration standards) (addition of internal standards to samples) Environmental Laboratories (preparation of calibration standards) (sample spiking) Food Testing Laboratories (preparation of calibration standards) (addition of internal standards to samples) These are example laboratories and applications evol applications are wide-spread Virtually every laboratory will see value in using evol

evol XR Kit evol XR Handle 5 ul evol Syringe 100 ul evol Syringe 1000 ul evol Syringe evol Stand Printed Quick Start Guide (English) Printed User Manual (English) Charger Information CD/USB Optional charging stand

evol XR Digital Analytical Syringe FEATURE Ergonomic, light, can be used with right or left hand BENEFIT Easy to use, operate all day Touch wheel, large colored screen Rapid menu selection, easy to use Digitally controlled aspirate and dispense Accuracy, reliability and precision

evol XR Digital Analytical Syringe FEATURE Colored syringes with unique batch number BENEFIT Easy use, easy identification, no risk of contamination, traceability Syringes with gas tight plunger (PTFE) as a positive displacement system Applicable for liquid/gas/viscous /aqueous/volatile samples with high accuracy and precision. Syringes with metal needles Can pierce vial septa reducing handling time and avoiding risk of contamination. Ensures minimal evaporation for volatile samples.

Support Services - Our Website Product Data Sheets and Part Number Lists

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Summary evol is the union of two precision devices; a digitally controlled electronic drive and XCHANGE analytical syringes All users are able to manually dispense any liquid precisely and accurately To comply with stringent laboratory standards (e.g. GLP, GMP, FDA) evol can be easily calibrated to ensure accurate dispensing at all times. evol is the perfect solution for routine repeat dispensing of all liquids, especially volatile solvents, hazardous chemicals, corrosive chemicals and viscous samples for a wide range of applications

Kinesis Ltd based in UK Appointed Middle East Distributors for Trajan s SGE range of products including evol. Please come to Stand 346 for a demonstration of the evol with our team and be eligible for Show Special price on evol XR Starter Kit!

Thank you for your attention. Questions?