INSTRUCTIONS PROCEDURE A: 1) Examine the diagram of perch chromosomes supplied. They have been removed from the nucleus of the white blood cell after replication. 2) Cut out each chromosome map of these 48 replicated chromosomes and arrange them on the worksheet with their other pair. Place the centromere of the replicated chromosomes on the lines of the worksheet. 3) Tape or glue down the arranged pairs in rows of 6. (Notice that a numbered chromosome will be paired with a lettered chromosome. 4) Answer the following questions: a) How many chromosomes are present in the spread or karyotype? b) Which pair of the chromosomes are sex chromosomes? c) What is the sex of this perch? d) What is the diploid or 2n number of a yellow perch? Yellow Perch Chromatid Centromere Telomere 1 of 12
karyotype yellow perch CUT OUT SHEET 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 A B C D E F G H I J K L M N O P Q R S T U V W X 2 of 12
KARYOTYPE FOR YELLOW PERCH 3 of 12
ANSWER KEY PROCEDURE A: 1) Examine the diagram of perch chromosomes supplied. They have been removed from the nucleus of the white blood cell after replication. 2) Cut out each chromosome map of these 48 replicated chromosomes and arrange them on the worksheet with their other pair. Place the centromere of the replicated chromosomes on the lines of the worksheet. 3) Tape or glue down the arranged pairs in rows of 6. (Notice that a numbered chromosome will be paired with a lettered chromosome. 4) Answer the following questions: a) How many chromosomes are present in the spread or karyotype? 48 b) Which pair of the chromosomes are sex chromosomes? 16 and I (the last pair) c) What is the sex of this perch? Female d) What is the diploid or 2n number of a yellow perch? 48 Yellow Perch Chromatid Centromere Telomere 4 of 12
ANSWER KEY KARYOTYPE FOR YELLOW PERCH 5 M 9 J 15 R 8 F 21 V 13 O 24 T 1 B 17 X 2 H 18 U 10 D 20 W 14 K 3 E 6 G 4 S 22 L 11 Q 7 A 12 19 N 23 P 16 I C 5 of 12
instructions PROCEDURE B: 1) Examine the diagram of perch chromosomes supplied. They have been removed from the nucleus of the white blood cell after replication. 2) Cut out each chromosome map of these 60 replicated chromosomes and arrange them on the worksheet with their other pair. Place the centromere of the replicated chromosomes on the lines of the worksheet. 3) Tape or glue down the arranged pairs in rows of 6. (Notice that a numbered chromosome will be paired with a lettered chromosome. 4) Answer the following questions: a) How many chromosomes does this fish posses in the nuclei of its cells? b) How many chromosomes would this fish have in its gametes or sex cells? c) What is the sex of this fish? d) Identify this fish by referring to the chart below which gives the chromosome numbers of five Manitoba fishes. Manitoba Fish Diploid Number Yellow Perch 48 Small Mouth Bass 46 Brown Bullhead 60 Walleye 50 Lake Trout 84 6 of 12
karyotype FISH B CUT OUT SHEET 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z AA BB CC DD 7 of 12
KARYOTYPE FOR FISH B 8 of 12
ANSWER KEY PROCEDURE B: 1) Examine the diagram of perch chromosomes supplied. They have been removed from the nucleus of the white blood cell after replication. 2) Cut out each chromosome map of these 60 replicated chromosomes and arrange them on the worksheet with their other pair. Place the centromere of the replicated chromosomes on the lines of the worksheet. 3) Tape or glue down the arranged pairs in rows of 6. (Notice that a numbered chromosome will be paired with a lettered chromosome. 4) Answer the following questions: a) How many chromosomes does this fish posses in the nuclei of its cells? 60 b) How many chromosomes would this fish have in its gametes or sex cells? 30 c) What is the sex of this fish? Male d) Identify this fish by referring to the chart below which gives the chromosome numbers of five Manitoba fishes. Manitoba Fish Diploid Number Yellow Perch 48 Small Mouth Bass 46 Brown Bullhead 60 Walleye 50 Lake Trout 84 9 of 12
ANSWER KEY KARYOTYPE FOR FISH B 18 K 6 C 20 Y 12 M 3 F 16 Q 11 I 1V 25 O 17 T 30 DD 23 B 29 G 24 A 8 N 14 BB 27 J 5 W 10 CC 4 S 28 Z 21 E 13 AA 26 L 19P 7U 15 D 2 X 9 R 22 H 10 of 12
INSTRUCTIONS PROCEDURE C: For further consideration. Compare the karyotypes of the two fishes studied in the above procedures (A and B) with a human karyotype. How are they similar, how are they different? HUMAN KARYOTYPE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 11 of 12
answer key PROCEDURE C: For further consideration. Compare the karyotypes of the two fishes studied in the above procedures (A and B) with a human karyotype. How are they similar, how are they different? The karyotypes of the fish studied are similar to a human karyotype since they both have chromosomes that are in pairs and they undergo meiosis. Also, the chromosomes are connected by centromeres and both humans and fish have sex chromosomes. The karyotypes of the fish studied are different than a human karyotype since a they have different diploid numbers. Also, the centromeres are in different positions. HUMAN KARYOTYPE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 12 of 12