I. Genes found on the same chromosome = linked genes
|
|
- Alison Parker
- 8 years ago
- Views:
Transcription
1 Genetic recombination in Eukaryotes: crossing over, part 1 I. Genes found on the same chromosome = linked genes II. III. Linkage and crossing over Crossing over & chromosome mapping I. Genes found on the same chromosome = linked genes Conflicting cytological evidence, only a few dozen chromosomes/individual so must be several genes per chromosome Testcross experiments revealed Conclusion: Genes assort independently if they are on different chromosomes but show linkage if they are on the same chromosome. 1
2 If a testcross is done and the genes are on separate chromosomes: Aa/Bb x aa/bb 2 genes, located on different chromosomes, will segregate independently. 2
3 Chromosome is the unit of transmission, not the gene Linkage = two or more genes located on the same chromosome Linked genes are not free to undergo independent assortment Instead, the alleles at all loci of one chromosome, should in theory, be transmitted as a unit during gamete formation. When two genes are compeletely linked, no crossing over occurs therefore, 3
4 II. Linkage and crossing over A. Crossing over breakage and rejoining process between Crossing over produces The % of recombinant gametes varies, dependent upon location of the loci. The closer the genes are, C.O. Breakage and rejoining process between two homologous non-sister chromatids, keep in mind: 4
5 Recombination Frequency (RF) = the # of recombinants/total progeny B. Recombination Frequency (RF), unlinked genes v. linked genes 1). In the case of unlinked genes, independent assortment holds true: Testcross: Heterozygous x homozygous mutant AaBb x aabb Offspring: So from a cross resulting in 100 progeny we would see 25 individuals from each genotype. the # of recombinants 5
6 2). In the case of linked genes, there is no independent assortment Testcross: Heterozygous x homozygous mutant AaBb x aabb Offspring: AaBb, aabb, Aabb, aabb So from a cross resulting in 100 progeny we would see a lot more of these two genotypes when compared to the recombinants: Crossing between adjacent non sister chromatids generates recombinants The two chromatids not involved in the exchange result in non-parental gametes 6
7 We can compare the RF to what one would expect with independent assortment RF Range Recombination by Crossing Over points to keep in mind: 1. CO s can occur between any two nonsister chromatids 2. If there is NO crossing over, only parental types will be observed 3. If there IS crossing over, RF will increase up to 50% 4. when the loci of two linked genes are very far apart, the RF approaches 50%, 1:1:1:1 ratio observed, thus transmission of the linked genes is indistinguishable from that of two unlinked genes 7
8 Morgan noted the proportion of recombinant progeny varied depending on which linked genes were being examined Testcross F1 results: pr + pr vg + vg x pr pr vg vg pr + vg pr vg 1195 pr + vg 151 pr vg y + y w + w x yy ww y w 43 y + w 2146 y w y + w + 22 As Morgan studied more linked genes, he saw that the proportion of recombinant progeny varied considerably. III. Chromosome mapping determined by analyzing Drosophila crosses Morgan hypothesized that variations in RF might indicate the actual distances separating genes on the chromosomes. Sturtevant (Morgan s student) compiled data on recombination between genes in Drosophila test crosses He found that the closer the two linked genes, the lower the recombination frequency- thus RF may be correlated with the map distance between two loci on a chromosome Alfred Sturtevant 8
9 A. Linkage Maps derived by Sturtevant Linkage of genes can be represented in the form of a genetic map, which shows the linear order of genes along a chromosome. The % recombinant offspring is correlated w/the distance between the two genes, thus the degree of crossing over between any two loci on a single chromosome is proportional to the distance between them, known as the interlocus distance Variations in recombination frequency indicate B. Map Units Map Unit (m.u.) = the distance between genes for which one product of meiosis out of 100 is recombinant [RF of 1% = 1 m.u. or 1 cm] e.g. if RF 12% between A & B, and 28% between B & C: A B C 9
10 Linkage map of Drosophila 4 linkage groups identified 10
11 F1: F2 males females F1 F2 males females 11
12 A plant of genotype: A B a b Is test-crossed to a b a b If the two loci are 10 m.u. apart, what proportion of progeny will be A B / a b? In the garden pea, orange pods (orp) are recessive to normal pods (Orp), and sensitivity to pea mosaic virus (mo) is recessive to resistance to the virus (Mo). A plant with orange pods and sensitivity is crossed to a true-breeding plant with normal pods and resistance. The F1 plants were then test-crossed to plants with orange pods and sensitivity. The following results were obtained: 160 orange pods/sensitive 165 normal pods/resistant 36 orange pods/resistant 39 normal pods/sensitive calculate the map distance between the two genes 12
13 C. Mapping multiple genes Threepoint mapping & Alfred s research Hypothesis = when multiple genes are located on the same chromosome, the distance between the genes can be estimated from the proportion of recombinant offspring. A. Sturtevant s First Genetic Map The linear order of these genes can be determined using testcross data Examined 5 different genes: y, w, v, m, r All alleles were found to be recessive and X linked. Crossed the double heterozygote female with hemizygous male recessive for the same alleles. Example: y+y w+w x yw y+w+ yw y+w yw+ RF = 214/21,736 = w+w r+r x wr w+r+ wr w+r wr+ RF = 2,062/6116 =
14 genes are arranged on the chromosome in a linear order- which can be determined The Complete Data: Alleles y and w y and v y and r y and m w and v w and r w and m v and r w and m # R./total# 214/21,736 1,464/4, / / /1,584 2,062/ /898 17/ /405 RF 1% 32.2% 35.5% 37.5% 29.7% 33.7% 45.2% 3% 26.9% 14
15 y-w = 1 m.u. v-r = 3 m.u. y-m = 37.5 m.u. w-r = 33.7 m.u. w-v = 29.7 m.u. Eukaryotic linkage, part 2 I. Three-point mapping to determine genetic maps A. Multiple cross-overs B. How to: analyzing the 3 pt testcross C. Mapping the results D. The accuracy of mapping E. Mitotic recombination and Sister Chromatid Exhanges II. Genetic mapping in haploid eukaryotes A. Ordered tetrad analysis B. Unordered tetrad analysis 15
16 I. Three-point mapping in Drosophila to determine genetic maps We can map 3 or more linked genes in a single cross, provided the following are true: The genotype of the organism producing crossover gametes must be heterozygous for all loci under consideration Offspring sample size must be high enough to recover a representative sample of all crossover classes DCOs (double crossovers) double exchanges of genetic material in two regions (RI & RII) RI RII Probability of a single crossover occurring between two loci is directly related to the distance separating the loci in the case of a DCO, two separate and independent crossovers must occur simultaneously. 16
17 three-point testcross for mapping Cross two true-breeding strains that differ with regard to three alleles to obtain F1 individuals that are heterozygous for all three alleles: y + y, w + w, ec + ec Perform a testcross by mating the F1 female to males that are homozygous recessive for all three alleles Results in 8 phenotypic classes (2 3 ) FI female: (heterozygous for 3 genes) her possible gametes: y w ec y+ w+ ec+ y w ec+ y w+ ec y w+ ec+ y+ w ec y+ w+ ec y+ w ec+ Because the F 2 phenotypes complement each other (i.e., one is wild type and the other is mutant for all three genes), they are called reciprocal classes of phenotypes. The distance between two genes in a three-point cross is equal to the percentage of all detectable exchanges occurring between them and includes all single and double crossovers. 17
18 How to: analyzing the 3 pt testcross Collect data from the F2 generation. Parental types (usually the two highest # s) Non-Parentals (recombinants) Double Crossovers (two lowest # s) Single Crossovers (two are RI, two are RII) Determine the gene order based upon the DCOs Calculate the RF for each region to determine the map distance between genes (#recombinants/total x 100) P: v + v +, cvcv, ctct x vv, cv + cv +, ct + ct + Testcross: v + v, cv + cv, ct + ct x vv, cvcv, ctct Phenotype v cv + ct + v + cv ct 592 v cv ct + 45 v + cv + ct 40 v cv ct 89 v + cv + ct + 94 v cv + ct 3 v + cv ct + 5 # offspring
19 Parental input Possible output t Only the first possibility is compatible with the data. Example: bb, prpr, vgvg x b + b, + pr + pr, + vg + vg + F1: b + b pr + pr vg + vg testcross: b + b pr + pr vg + vg x bb prpr vgvg Phenotype b + pr + vg + b pr vg b + pr vg 30 b pr + vg + 28 b + pr + vg 61 b pr vg + 60 b + pr vg + 2 b pr + vg # observed Distance between b & pr = Distance between pr & vg = 19
20 Mapping the results: The eye color gene must be in the middle. This order of genes is confirmed by the pattern of traits found in the double crossovers. Double crossover data b + pr vg + 2 b pr + vg 1 D. The accuracy of mapping: Interference Interference = When a crossover in one region affects the likelihood of there being a crossover in an adjacent region Expected frequency of DCOs DCOs rare between segments that are very short If crossovers in the 2 regions are independent, then: frequency of double recombinants = product of the recombinant frequencies in the adjacent regions Expected DCOs = x = x 1,005 = 7.5 Coefficient of coincidence (c.o.c) Observed/expected double recombinants 1 3/7.5 = 0.4 I = 1-c.o.c 20
21 Step by step summary: 1. Calculate the RF for each pair of genes 2. Draw the linkage map 3. Determine the double recombinants 4. Calculate the Frequency & # of double recombinants expected if there is no interference 5. Calculate Interference Problem: Vermilion eyes are recessive to normal, miniature wings are recessive to long wings, and sable body is recessive to gray body. A cross was made between a heterozygous female for all three genes and a homozygous recessive male. Data: 1,320 vermilion eyes, miniature wings, sable body 1,346 red eyes, long wings, gray body 102 vermilion eyes, miniature wings, gray body 90 red eyes, long wings, sable body 42 vermilion eyes, long wings, gray body 48 red eyes, miniature wings, sable body 2 vermilion eyes, long wings, sable body 1 red eyes, miniature wings, gray body A. Determine the gene order & calculate the map distance between the three genes B. Calculate interference 21
22 E. Mitotic recombination and Sister Chromatid Exhanges Mitotic recombination = crossing over that occurs during mitosis (it does happen, in often in Drosophila & fungi also in humans & mice) It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences Those between non-sister chromatids producemutant patches in female flies = twin spot SCE s don t produce new allelic combinations, but may be involved in repairing DNA lesions II. Genetic mapping in haploid eukaryotes Fungi haploid (n) multicellular organisms that can reproduce asexually to create a diploid zygote Diploid zygote proceeds through meiosis to produce four haploid spores = ascospores 22
23 23
24 Group of four spores is known as a tetrad In some species meiosis is followed by mitosis to produce eight cells known as an octad. Ascus = sac that contains the tetrad/octad Can do an ordered tetrad analysis because the 8 cells reflect the sequence of formation what s the fun in fungi? They are haploid They produce large # s of progeny They have short life cycles Can make direct observations on the behavior of genes during meiosis, can examine cross-overs, can map centromeres Chlamydomonas Neurospora 24
25 A. Ordered tetrad analysis: Linear tetrad analysis can be used to map distance between a gene and the centromere. 1). FDS PATTERN: 2) SDS PATTERN: a) 2:2:2:2 b) 2:4:2 segregate until the 2 nd meitotic division is complete! 25
26 Calculating map distance w/ ordered tetrads mapping the centromere - % of SDS or M2 asci can be used to calculate the map distance between the centromere & the gene of interest Map distance = B. Unordered tetrad analysis can be used to map genes in dihybrid crosses, Spores are randomly arranged Haploid cell AB x ab Haploid cell Aa Bb Diploid zygote meiosis AB AB AB Ab Ab Ab ab ab ab ab ab ab Parental ditype Tetratype Nonparental ditype 26
27 When two genes are located on different chromosomes: 27
28 When two genes are located on the same chromosome: Map distance = (1/2) ([TT] + 3[NPD]) + 4 [NPD] / total x 100 If the # of parentals = nonparentals, the two are unlinked If there are TT s, CO s have occurred, if there are NPDs then DCO s occurred. The following spore arrangements were obtained from tetrads in a cross between Neurospora strain com val (c v) and a wild type strain (+ +). Only 1 member of each pair of spores is shown. Spore pair Ascus composition 1-2 cv c+ cv +v cv 3-4 cv c+ c+ c+ +v v +v cv c v Number: What can you conclude about linkage? 35 PD 36 NPD 30 TT PD = NPD, genes not linked 28
5 GENETIC LINKAGE AND MAPPING
5 GENETIC LINKAGE AND MAPPING 5.1 Genetic Linkage So far, we have considered traits that are affected by one or two genes, and if there are two genes, we have assumed that they assort independently. However,
More informationChapter 9 Patterns of Inheritance
Bio 100 Patterns of Inheritance 1 Chapter 9 Patterns of Inheritance Modern genetics began with Gregor Mendel s quantitative experiments with pea plants History of Heredity Blending theory of heredity -
More informationName: Class: Date: ID: A
Name: Class: _ Date: _ Meiosis Quiz 1. (1 point) A kidney cell is an example of which type of cell? a. sex cell b. germ cell c. somatic cell d. haploid cell 2. (1 point) How many chromosomes are in a human
More informationChapter 13: Meiosis and Sexual Life Cycles
Name Period Chapter 13: Meiosis and Sexual Life Cycles Concept 13.1 Offspring acquire genes from parents by inheriting chromosomes 1. Let s begin with a review of several terms that you may already know.
More information5. The cells of a multicellular organism, other than gametes and the germ cells from which it develops, are known as
1. True or false? The chi square statistical test is used to determine how well the observed genetic data agree with the expectations derived from a hypothesis. True 2. True or false? Chromosomes in prokaryotic
More informationChromosomes, Mapping, and the Meiosis Inheritance Connection
Chromosomes, Mapping, and the Meiosis Inheritance Connection Carl Correns 1900 Chapter 13 First suggests central role for chromosomes Rediscovery of Mendel s work Walter Sutton 1902 Chromosomal theory
More informationLecture 2: Mitosis and meiosis
Lecture 2: Mitosis and meiosis 1. Chromosomes 2. Diploid life cycle 3. Cell cycle 4. Mitosis 5. Meiosis 6. Parallel behavior of genes and chromosomes Basic morphology of chromosomes telomere short arm
More informationThe correct answer is c A. Answer a is incorrect. The white-eye gene must be recessive since heterozygous females have red eyes.
1. Why is the white-eye phenotype always observed in males carrying the white-eye allele? a. Because the trait is dominant b. Because the trait is recessive c. Because the allele is located on the X chromosome
More informationChapter 13: Meiosis and Sexual Life Cycles
Name Period Concept 13.1 Offspring acquire genes from parents by inheriting chromosomes 1. Let s begin with a review of several terms that you may already know. Define: gene locus gamete male gamete female
More informationBio EOC Topics for Cell Reproduction: Bio EOC Questions for Cell Reproduction:
Bio EOC Topics for Cell Reproduction: Asexual vs. sexual reproduction Mitosis steps, diagrams, purpose o Interphase, Prophase, Metaphase, Anaphase, Telophase, Cytokinesis Meiosis steps, diagrams, purpose
More informationPractice Problems 4. (a) 19. (b) 36. (c) 17
Chapter 10 Practice Problems Practice Problems 4 1. The diploid chromosome number in a variety of chrysanthemum is 18. What would you call varieties with the following chromosome numbers? (a) 19 (b) 36
More informationList, describe, diagram, and identify the stages of meiosis.
Meiosis and Sexual Life Cycles In this topic we will examine a second type of cell division used by eukaryotic cells: meiosis. In addition, we will see how the 2 types of eukaryotic cell division, mitosis
More informationA and B are not absolutely linked. They could be far enough apart on the chromosome that they assort independently.
Name Section 7.014 Problem Set 5 Please print out this problem set and record your answers on the printed copy. Answers to this problem set are to be turned in to the box outside 68-120 by 5:00pm on Friday
More information1. Why is mitosis alone insufficient for the life cycle of sexually reproducing eukaryotes?
Chapter 13: Meiosis and Sexual Life Cycles 1. Why is mitosis alone insufficient for the life cycle of sexually reproducing eukaryotes? 2. Define: gamete zygote meiosis homologous chromosomes diploid haploid
More informationHeredity - Patterns of Inheritance
Heredity - Patterns of Inheritance Genes and Alleles A. Genes 1. A sequence of nucleotides that codes for a special functional product a. Transfer RNA b. Enzyme c. Structural protein d. Pigments 2. Genes
More informationBiology 1406 Exam 4 Notes Cell Division and Genetics Ch. 8, 9
Biology 1406 Exam 4 Notes Cell Division and Genetics Ch. 8, 9 Ch. 8 Cell Division Cells divide to produce new cells must pass genetic information to new cells - What process of DNA allows this? Two types
More informationCCR Biology - Chapter 7 Practice Test - Summer 2012
Name: Class: Date: CCR Biology - Chapter 7 Practice Test - Summer 2012 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. A person who has a disorder caused
More informationName: 4. A typical phenotypic ratio for a dihybrid cross is a) 9:1 b) 3:4 c) 9:3:3:1 d) 1:2:1:2:1 e) 6:3:3:6
Name: Multiple-choice section Choose the answer which best completes each of the following statements or answers the following questions and so make your tutor happy! 1. Which of the following conclusions
More informationHeredity. Sarah crosses a homozygous white flower and a homozygous purple flower. The cross results in all purple flowers.
Heredity 1. Sarah is doing an experiment on pea plants. She is studying the color of the pea plants. Sarah has noticed that many pea plants have purple flowers and many have white flowers. Sarah crosses
More information(1-p) 2. p(1-p) From the table, frequency of DpyUnc = ¼ (p^2) = #DpyUnc = p^2 = 0.0004 ¼(1-p)^2 + ½(1-p)p + ¼(p^2) #Dpy + #DpyUnc
Advanced genetics Kornfeld problem set_key 1A (5 points) Brenner employed 2-factor and 3-factor crosses with the mutants isolated from his screen, and visually assayed for recombination events between
More informationLAB 8 EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS
LAB 8 EUKARYOTIC CELL DIVISION: MITOSIS AND MEIOSIS Los Angeles Mission College Biology 3 Name: Date: INTRODUCTION BINARY FISSION: Prokaryotic cells (bacteria) reproduce asexually by binary fission. Bacterial
More informationThe cell cycle, mitosis and meiosis
The cell cycle, mitosis and meiosis Learning objective This learning material is about the life cycle of a cell and the series of stages by which genetic materials are duplicated and partitioned to produce
More informationGenetics Lecture Notes 7.03 2005. Lectures 1 2
Genetics Lecture Notes 7.03 2005 Lectures 1 2 Lecture 1 We will begin this course with the question: What is a gene? This question will take us four lectures to answer because there are actually several
More informationGENETIC CROSSES. Monohybrid Crosses
GENETIC CROSSES Monohybrid Crosses Objectives Explain the difference between genotype and phenotype Explain the difference between homozygous and heterozygous Explain how probability is used to predict
More informationWorkshop: Cellular Reproduction via Mitosis & Meiosis
Workshop: Cellular Reproduction via Mitosis & Meiosis Introduction In this workshop you will examine how cells divide, including how they partition their genetic material (DNA) between the two resulting
More informationCHROMOSOMES AND INHERITANCE
SECTION 12-1 REVIEW CHROMOSOMES AND INHERITANCE VOCABULARY REVIEW Distinguish between the terms in each of the following pairs of terms. 1. sex chromosome, autosome 2. germ-cell mutation, somatic-cell
More informationCell Growth and Reproduction Module B, Anchor 1
Cell Growth and Reproduction Module B, Anchor 1 Key Concepts: - The larger a cell becomes, the more demands the cell places on its DNA. In addition, a larger cell is less efficient in moving nutrients
More informationCHROMOSOME STRUCTURE CHROMOSOME NUMBERS
CHROMOSOME STRUCTURE 1. During nuclear division, the DNA (as chromatin) in a Eukaryotic cell's nucleus is coiled into very tight compact structures called chromosomes. These are rod-shaped structures made
More information2 GENETIC DATA ANALYSIS
2.1 Strategies for learning genetics 2 GENETIC DATA ANALYSIS We will begin this lecture by discussing some strategies for learning genetics. Genetics is different from most other biology courses you have
More information1. You are studying three autosomal recessive mutations in the fruit fly Drosophila
7.03 Exams Archives 1 of 126 Exam Questions from Exam 1 Basic Genetic Tests, Setting up and Analyzing Crosses, and Genetic Mapping 1. You are studying three autosomal recessive mutations in the fruit fly
More informationBioSci 2200 General Genetics Problem Set 1 Answer Key Introduction and Mitosis/ Meiosis
BioSci 2200 General Genetics Problem Set 1 Answer Key Introduction and Mitosis/ Meiosis Introduction - Fields of Genetics To answer the following question, review the three traditional subdivisions of
More informationBiology Final Exam Study Guide: Semester 2
Biology Final Exam Study Guide: Semester 2 Questions 1. Scientific method: What does each of these entail? Investigation and Experimentation Problem Hypothesis Methods Results/Data Discussion/Conclusion
More information1. When new cells are formed through the process of mitosis, the number of chromosomes in the new cells
Cell Growth and Reproduction 1. When new cells are formed through the process of mitosis, the number of chromosomes in the new cells A. is half of that of the parent cell. B. remains the same as in the
More informationBio 102 Practice Problems Mendelian Genetics and Extensions
Bio 102 Practice Problems Mendelian Genetics and Extensions Short answer (show your work or thinking to get partial credit): 1. In peas, tall is dominant over dwarf. If a plant homozygous for tall is crossed
More informationSexual Reproduction. The specialized cells that are required for sexual reproduction are known as. And come from the process of: GAMETES
Sexual Reproduction Sexual Reproduction We know all about asexual reproduction 1. Only one parent required. 2. Offspring are identical to parents. 3. The cells that produce the offspring are not usually
More informationAP: LAB 8: THE CHI-SQUARE TEST. Probability, Random Chance, and Genetics
Ms. Foglia Date AP: LAB 8: THE CHI-SQUARE TEST Probability, Random Chance, and Genetics Why do we study random chance and probability at the beginning of a unit on genetics? Genetics is the study of inheritance,
More informationBioBoot Camp Genetics
BioBoot Camp Genetics BIO.B.1.2.1 Describe how the process of DNA replication results in the transmission and/or conservation of genetic information DNA Replication is the process of DNA being copied before
More informationA trait is a variation of a particular character (e.g. color, height). Traits are passed from parents to offspring through genes.
1 Biology Chapter 10 Study Guide Trait A trait is a variation of a particular character (e.g. color, height). Traits are passed from parents to offspring through genes. Genes Genes are located on chromosomes
More informationChromosomal Basis of Inheritance. Ch. 3
Chromosomal Basis of Inheritance Ch. 3 THE CHROMOSOME THEORY OF INHERITANCE AND SEX CHROMOSOMES! The chromosome theory of inheritance describes how the transmission of chromosomes account for the Mendelian
More informationMeiosis is a special form of cell division.
Page 1 of 6 KEY CONCEPT Meiosis is a special form of cell division. BEFORE, you learned Mitosis produces two genetically identical cells In sexual reproduction, offspring inherit traits from both parents
More informationBiology 1406 - Notes for exam 5 - Population genetics Ch 13, 14, 15
Biology 1406 - Notes for exam 5 - Population genetics Ch 13, 14, 15 Species - group of individuals that are capable of interbreeding and producing fertile offspring; genetically similar 13.7, 14.2 Population
More informationScience 10-Biology Activity 14 Worksheet on Sexual Reproduction
Science 10-Biology Activity 14 Worksheet on Sexual Reproduction 10 Name Due Date Show Me NOTE: This worksheet is based on material from pages 367-372 in Science Probe. 1. Sexual reproduction requires parents,
More informationLecture 7 Mitosis & Meiosis
Lecture 7 Mitosis & Meiosis Cell Division Essential for body growth and tissue repair Interphase G 1 phase Primary cell growth phase S phase DNA replication G 2 phase Microtubule synthesis Mitosis Nuclear
More informationBasic Principles of Forensic Molecular Biology and Genetics. Population Genetics
Basic Principles of Forensic Molecular Biology and Genetics Population Genetics Significance of a Match What is the significance of: a fiber match? a hair match? a glass match? a DNA match? Meaning of
More informationBiology Behind the Crime Scene Week 4: Lab #4 Genetics Exercise (Meiosis) and RFLP Analysis of DNA
Page 1 of 5 Biology Behind the Crime Scene Week 4: Lab #4 Genetics Exercise (Meiosis) and RFLP Analysis of DNA Genetics Exercise: Understanding how meiosis affects genetic inheritance and DNA patterns
More informationThe Genetics of Drosophila melanogaster
The Genetics of Drosophila melanogaster Thomas Hunt Morgan, a geneticist who worked in the early part of the twentieth century, pioneered the use of the common fruit fly as a model organism for genetic
More informationMendelian and Non-Mendelian Heredity Grade Ten
Ohio Standards Connection: Life Sciences Benchmark C Explain the genetic mechanisms and molecular basis of inheritance. Indicator 6 Explain that a unit of hereditary information is called a gene, and genes
More informationChapter 8: Variation in Chromosome Structure and Number
Chapter 8: Variation in Chromosome Structure and Number Student Learning Objectives Upon completion of this chapter you should be able to: 1. Know the principles and terminology associated with variations
More informationBIO 184 Page 1 Spring 2013 NAME VERSION 1 EXAM 3: KEY. Instructions: PRINT your Name and Exam version Number on your Scantron
BIO 184 Page 1 Spring 2013 EXAM 3: KEY Instructions: PRINT your Name and Exam version Number on your Scantron Example: PAULA SMITH, EXAM 2 VERSION 1 Write your name CLEARLY at the top of every page of
More information4.2 Meiosis. Meiosis is a reduction division. Assessment statements. The process of meiosis
4.2 Meiosis Assessment statements State that meiosis is a reduction division of a diploid nucleus to form haploid nuclei. Define homologous chromosomes. Outline the process of meiosis, including pairing
More informationGenetics Module B, Anchor 3
Genetics Module B, Anchor 3 Key Concepts: - An individual s characteristics are determines by factors that are passed from one parental generation to the next. - During gamete formation, the alleles for
More informationLAB : THE CHI-SQUARE TEST. Probability, Random Chance, and Genetics
Period Date LAB : THE CHI-SQUARE TEST Probability, Random Chance, and Genetics Why do we study random chance and probability at the beginning of a unit on genetics? Genetics is the study of inheritance,
More informationTwo copies of each autosomal gene affect phenotype.
SECTION 7.1 CHROMOSOMES AND PHENOTYPE Study Guide KEY CONCEPT The chromosomes on which genes are located can affect the expression of traits. VOCABULARY carrier sex-linked gene X chromosome inactivation
More informationGene Mapping Techniques
Gene Mapping Techniques OBJECTIVES By the end of this session the student should be able to: Define genetic linkage and recombinant frequency State how genetic distance may be estimated State how restriction
More informationB2 5 Inheritrance Genetic Crosses
B2 5 Inheritrance Genetic Crosses 65 minutes 65 marks Page of 55 Q. A woman gives birth to triplets. Two of the triplets are boys and the third is a girl. The triplets developed from two egg cells released
More informationDeterministic computer simulations were performed to evaluate the effect of maternallytransmitted
Supporting Information 3. Host-parasite simulations Deterministic computer simulations were performed to evaluate the effect of maternallytransmitted parasites on the evolution of sex. Briefly, the simulations
More informationCHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE. Section B: Sex Chromosomes
CHAPTER 15 THE CHROMOSOMAL BASIS OF INHERITANCE Section B: Sex Chromosomes 1. The chromosomal basis of sex varies with the organism 2. Sex-linked genes have unique patterns of inheritance 1. The chromosomal
More informationBio 101 Section 001: Practice Questions for First Exam
Do the Practice Exam under exam conditions. Time yourself! MULTIPLE CHOICE: 1. The substrate fits in the of an enzyme: (A) allosteric site (B) active site (C) reaction groove (D) Golgi body (E) inhibitor
More informationwww.njctl.org PSI Biology Mitosis & Meiosis
Mitosis and Meiosis Mitosis Classwork 1. Identify two differences between meiosis and mitosis. 2. Provide an example of a type of cell in the human body that would undergo mitosis. 3. Does cell division
More informationCHAPTER 10 CELL CYCLE AND CELL DIVISION
CHAPTER 10 CELL CYCLE AND CELL DIVISION Cell division is an inherent property of living organisms. It is a process in which cells reproduce their own kind. The growth, differentiation, reproduction and
More informationP1 Gold X Black. 100% Black X. 99 Black and 77 Gold. Critical Values 3.84 5.99 7.82 9.49 11.07 12.59 14.07 15.51
Questions for Exam I Fall 2005 1. Wild-type humbugs have no spots, have red eyes and brown bodies. You have isolated mutations in three new autosomal humbug genes. The mutation Sp gives a dominant phenotype
More informationGenetics 1. Defective enzyme that does not make melanin. Very pale skin and hair color (albino)
Genetics 1 We all know that children tend to resemble their parents. Parents and their children tend to have similar appearance because children inherit genes from their parents and these genes influence
More informationEx) A tall green pea plant (TTGG) is crossed with a short white pea plant (ttgg). TT or Tt = tall tt = short GG or Gg = green gg = white
Worksheet: Dihybrid Crosses U N I T 3 : G E N E T I C S STEP 1: Determine what kind of problem you are trying to solve. STEP 2: Determine letters you will use to specify traits. STEP 3: Determine parent
More informationTwo-locus population genetics
Two-locus population genetics Introduction So far in this course we ve dealt only with variation at a single locus. There are obviously many traits that are governed by more than a single locus in whose
More informationLAB : PAPER PET GENETICS. male (hat) female (hair bow) Skin color green or orange Eyes round or square Nose triangle or oval Teeth pointed or square
Period Date LAB : PAPER PET GENETICS 1. Given the list of characteristics below, you will create an imaginary pet and then breed it to review the concepts of genetics. Your pet will have the following
More information17. A testcross A.is used to determine if an organism that is displaying a recessive trait is heterozygous or homozygous for that trait. B.
ch04 Student: 1. Which of the following does not inactivate an X chromosome? A. Mammals B. Drosophila C. C. elegans D. Humans 2. Who originally identified a highly condensed structure in the interphase
More informationSexual Reproduction and Meiosis
12 Sexual Reproduction and Meiosis Concept Outline 12.1 Meiosis produces haploid cells from diploid cells. Discovery of Reduction Division. Sexual reproduction does not increase chromosome number because
More informationProblems 1-6: In tomato fruit, red flesh color is dominant over yellow flesh color, Use R for the Red allele and r for the yellow allele.
Genetics Problems Name ANSWER KEY Problems 1-6: In tomato fruit, red flesh color is dominant over yellow flesh color, Use R for the Red allele and r for the yellow allele. 1. What would be the genotype
More informationDNA Determines Your Appearance!
DNA Determines Your Appearance! Summary DNA contains all the information needed to build your body. Did you know that your DNA determines things such as your eye color, hair color, height, and even the
More informationSimulation Model of Mating Behavior in Flies
Simulation Model of Mating Behavior in Flies MEHMET KAYIM & AYKUT Ecological and Evolutionary Genetics Lab. Department of Biology, Middle East Technical University International Workshop on Hybrid Systems
More informationTerms: The following terms are presented in this lesson (shown in bold italics and on PowerPoint Slides 2 and 3):
Unit B: Understanding Animal Reproduction Lesson 4: Understanding Genetics Student Learning Objectives: Instruction in this lesson should result in students achieving the following objectives: 1. Explain
More informationMendelian Genetics in Drosophila
Mendelian Genetics in Drosophila Lab objectives: 1) To familiarize you with an important research model organism,! Drosophila melanogaster. 2) Introduce you to normal "wild type" and various mutant phenotypes.
More informationInfluence of Sex on Genetics. Chapter Six
Influence of Sex on Genetics Chapter Six Humans 23 Autosomes Chromosomal abnormalities very severe Often fatal All have at least one X Deletion of X chromosome is fatal Males = heterogametic sex XY Females
More informationMCB41: Second Midterm Spring 2009
MCB41: Second Midterm Spring 2009 Before you start, print your name and student identification number (S.I.D) at the top of each page. There are 7 pages including this page. You will have 50 minutes for
More information2 18. If a boy s father has haemophilia and his mother has one gene for haemophilia. What is the chance that the boy will inherit the disease? 1. 0% 2
1 GENETICS 1. Mendel is considered to be lucky to discover the laws of inheritance because 1. He meticulously analyzed his data statistically 2. He maintained pedigree records of various generations he
More informationCCpp X ccpp. CcPp X CcPp. CP Cp cp cp. Purple. White. Purple CcPp. Purple Ccpp White. White. Summary: 9/16 purple, 7/16 white
P F 1 CCpp X ccpp Cp Cp CcPp X CcPp F 2 CP Cp cp cp CP Cp cp cp CCPP CCPp CcPP CcPp CCPp CCpp CcPp Ccpp CcPP CcPp ccpp ccpp Summary: 9/16 purple, 7/16 white CcPp Ccpp ccpp ccpp AABB X aabb P AB ab Gametes
More information7A The Origin of Modern Genetics
Life Science Chapter 7 Genetics of Organisms 7A The Origin of Modern Genetics Genetics the study of inheritance (the study of how traits are inherited through the interactions of alleles) Heredity: the
More informationTest Two Study Guide
Test Two Study Guide 1. Describe what is happening inside a cell during the following phases (pictures may help but try to use words): Interphase: : Consists of G1 / S / G2. Growing stage, cell doubles
More informationGenetics 301 Sample Final Examination Spring 2003
Genetics 301 Sample Final Examination Spring 2003 50 Multiple Choice Questions-(Choose the best answer) 1. A cross between two true breeding lines one with dark blue flowers and one with bright white flowers
More informationChapter 3. Chapter Outline. Chapter Outline 9/11/10. Heredity and Evolu4on
Chapter 3 Heredity and Evolu4on Chapter Outline The Cell DNA Structure and Function Cell Division: Mitosis and Meiosis The Genetic Principles Discovered by Mendel Mendelian Inheritance in Humans Misconceptions
More informationPre-lab homework Lab 2: Reproduction in Protists, Fungi, Moss and Ferns
Pre-lab homework Lab 2: Reproduction in Protists, Fungi, Moss and Ferns Lab Section: Name: 1. Last week in lab you looked at the reproductive cycle of the animals. This week s lab examines the cycles of
More informationHardy-Weinberg Equilibrium Problems
Hardy-Weinberg Equilibrium Problems 1. The frequency of two alleles in a gene pool is 0.19 (A) and 0.81(a). Assume that the population is in Hardy-Weinberg equilibrium. (a) Calculate the percentage of
More informationPLANT EVOLUTION DISPLAY Handout
PLANT EVOLUTION DISPLAY Handout Name: TA and Section time Welcome to UCSC Greenhouses. This sheet explains a few botanical facts about plant reproduction that will help you through the display and handout.
More informationsomatic cell egg genotype gamete polar body phenotype homologous chromosome trait dominant autosome genetics recessive
CHAPTER 6 MEIOSIS AND MENDEL Vocabulary Practice somatic cell egg genotype gamete polar body phenotype homologous chromosome trait dominant autosome genetics recessive CHAPTER 6 Meiosis and Mendel sex
More informationIntroduction. What is Ecological Genetics?
1 Introduction What is Ecological enetics? Ecological genetics is at the interface of ecology, evolution, and genetics, and thus includes important elements from each of these fields. We can use two closely
More informationAnswer Key Problem Set 5
7.03 Fall 2003 1 of 6 1. a) Genetic properties of gln2- and gln 3-: Answer Key Problem Set 5 Both are uninducible, as they give decreased glutamine synthetase (GS) activity. Both are recessive, as mating
More informationChromosome Mapping Assignment INSTRUCTIONS
INSTRUCTIONS PROCEDURE A: 1) Examine the diagram of perch chromosomes supplied. They have been removed from the nucleus of the white blood cell after replication. 2) Cut out each chromosome map of these
More informationChapter 3. Cell Division. Laboratory Activities Activity 3.1: Mock Mitosis Activity 3.2: Mitosis in Onion Cells Activity 3.
Chapter 3 Cell Division Laboratory Activities Activity 3.1: Mock Mitosis Activity 3.2: Mitosis in Onion Cells Activity 3.3: Mock Meiosis Goals Following this exercise students should be able to Recognize
More informationForensic DNA Testing Terminology
Forensic DNA Testing Terminology ABI 310 Genetic Analyzer a capillary electrophoresis instrument used by forensic DNA laboratories to separate short tandem repeat (STR) loci on the basis of their size.
More informationMitosis, Meiosis and Fertilization 1
Mitosis, Meiosis and Fertilization 1 I. Introduction When you fall and scrape the skin off your hands or knees, how does your body make new skin cells to replace the skin cells that were scraped off? How
More informationDrosophila Genetics by Michael Socolich May, 2003
Drosophila Genetics by Michael Socolich May, 2003 I. General Information and Fly Husbandry II. Nomenclature III. Genetic Tools Available to the Fly Geneticists IV. Example Crosses V. P-element Transformation
More informationEvolution (18%) 11 Items Sample Test Prep Questions
Evolution (18%) 11 Items Sample Test Prep Questions Grade 7 (Evolution) 3.a Students know both genetic variation and environmental factors are causes of evolution and diversity of organisms. (pg. 109 Science
More informationGenetic Technology. Name: Class: Date: Multiple Choice Identify the choice that best completes the statement or answers the question.
Name: Class: Date: Genetic Technology Multiple Choice Identify the choice that best completes the statement or answers the question. 1. An application of using DNA technology to help environmental scientists
More informationHuman Blood Types: Codominance and Multiple Alleles. Codominance: both alleles in the heterozygous genotype express themselves fully
Human Blood Types: Codominance and Multiple Alleles Codominance: both alleles in the heterozygous genotype express themselves fully Multiple alleles: three or more alleles for a trait are found in the
More informationIncomplete Dominance and Codominance
Name: Date: Period: Incomplete Dominance and Codominance 1. In Japanese four o'clock plants red (R) color is incompletely dominant over white (r) flowers, and the heterozygous condition (Rr) results in
More informationPRACTICE PROBLEMS - PEDIGREES AND PROBABILITIES
PRACTICE PROBLEMS - PEDIGREES AND PROBABILITIES 1. Margaret has just learned that she has adult polycystic kidney disease. Her mother also has the disease, as did her maternal grandfather and his younger
More informationActual Quiz 1 (closed book) will be given Monday10/4 at 10:00 am
MIT Biology Department 7.012: Introductory Biology Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. laudette Gardel 7.012 Practice Quiz 1 Actual Quiz 1 (closed book) will
More informationGenetics and Evolution: An ios Application to Supplement Introductory Courses in. Transmission and Evolutionary Genetics
G3: Genes Genomes Genetics Early Online, published on April 11, 2014 as doi:10.1534/g3.114.010215 Genetics and Evolution: An ios Application to Supplement Introductory Courses in Transmission and Evolutionary
More informationPopulation Genetics and Multifactorial Inheritance 2002
Population Genetics and Multifactorial Inheritance 2002 Consanguinity Genetic drift Founder effect Selection Mutation rate Polymorphism Balanced polymorphism Hardy-Weinberg Equilibrium Hardy-Weinberg Equilibrium
More informationChapter 4 The role of mutation in evolution
Chapter 4 The role of mutation in evolution Objective Darwin pointed out the importance of variation in evolution. Without variation, there would be nothing for natural selection to act upon. Any change
More information