XPure. Simulated Moving Bed. www.xendo.com. System for Chromatography and Ion exchange applications. Better solutions for your purification needs

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XPure Simulated Moving Bed System for Chromatography and Ion exchange applications Better solutions for your purification needs www.xendo.com

... is an independent engineering and consulting company, was founded in 1990 and employs around 90 highly skilled professionals... is leading in expert consultancy offering integrated services in life sciences, pharmaceutical, healthcare and food industry for: project management process development engineering and design, qualification, validation compliance, quality assurance regulatory affairs Xendo Bio Science Park

Xendo pilot static SMB XPure-S with zero dead leg valve modules XENDO ENGINEERING Xendo s engineering and technical services support the full life cycle of process equipment and (Bio) pharmaceutical facilities. From project initiation, through design and construction, our engineers offer you a pragmatic approach to build specific process equipment and (GMP compliant) facilities. Our Maintenance support and Asset Management services assist you in keeping your equipment and facilities fit for purpose in a cost effective way. Depending on the needs of your project we operate from multidisciplinary teams at our Leiden office or provide you with professional support at site. Xendo SMB XPure engineering services Range from lab, pilot to industrial scale SMB systems (up to 150m 3 resin inventory) Covers complete design cycle - characterization - sorbent screening - pilot testing (on site) Covers sugars, food, food and non-food ingredients, (bio)pharmaceuticals, API s and fine chemicals hydrometallurgy Rely on state-of-the-art (good) engineering and automation practices and are automated to a steadystate operating process producing constant high quality product streams Xendo supports you to evaluate different techniques for: Purification Recovery Isolation of products As SMB system supplier Xendo delivers two state-of-theart technology concepts of SMB: XPure-S and XPure-C.

XPURE The static SMB system XPure-S that represents the by Xendo patented valve system with a zero dead leg concept. The carrousel SMB system XPure-C featuring a turn table that carries the sorption containers going through the distinct process steps of a typical IXchromatography cycle. Xendo offers SMB technology for product recovery and purification, comprising of Feasibility studies Developed simulation modelling software Full scale design Start-up, validation, maintenance and (process) service and Is not related to any manufacturer of resins Applies originally manufactured components or any fabricates that are consistent with clients material standards Optional elements - vessels and liquid distributors - (inter)connecting piping and auxiliary equipment Xendo Static SMB XPure-S during construction work Chromatography principles The chromatography principles can be divided in two phenomena: elution and fractionating chromatography. The chromatographic mode to be used is based on the binding characteristics of the ingredient. Fractionating Elution Affinity Ion exchange Size exclusion Reverse phase

V Fixed bed MTZ ф L ф L ф L MTZ exploited MTZ with concentration profile Wash Elution Wash Fixed bed configuration with Mass Transfer Zone (MTZ) evoluting into SMB concept Fixed bed versus SMB in Ion Exchange and Chromatography Two basic configuration concepts, fixed bed and continuous concept: Stationary or fixed bed configuration - one or multiple beds - (redundant) parallel or serial configuration. Continuous concept where multiple beds are processed simultaneously in different process modes. Process comparison Fixed Bed and SMB chromatography: Aspect Fixed bed Continuous Treatment ratio High (large BedVolume number) Medium/low (typically <25 BV s) Value product Low/medium High Resin unit cost Low/medium Medium/high Economical comparison Fixed Bed and SMB chromatography: Aspect Fixed bed SMB Resin inventory Large Medium/low Effective use of resin Medium/low High Process Batch Continuous Cycle time Long Short to medium Redundancy High No CAPEX Low/medium Medium/high Resin investment High Substantially lower Chemical and water consumption High Substantially lower Process inand outlets Columns CTRL Modular Fluid Distribution System Columns Schematic representation of an 8 column static SMB system with its valve blocks.

Columns of Xendo pilot carrousel XPure-C SUMMARY Xendo can cut your chromatography process costs by 20-50% Less stationary phase (sorbent) media (resins) by higher utilization, that result in significantly - reduction in buffer consumption (countercurrent contact) - lower water consumption - less energy Due to the modular characteristics of the valve blocks, Xendo can assemble these in a fast and efficient way All this makes Xendo SMB technology attractive in terms of Design Cost Delivery time Smooth (after sales) services. Xendo has experience Over 200 SMB studies Over 30 of industrial projects realised If you are interested in any of the above services, please contact: Xendo B.V. Bio Science park Archimedesweg 17 2333 CM Leiden tel: +31 (0)71 524 4000 P.O. Box 255 2300 AG Leiden The Netherlands Menne Zaalberg email: menne.zaalberg@xendo.com Pieter de Wit email: pieter.de.wit@xendo.com