LEADERS: 5-Year Follow-up



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LEADERS: -Year Follow-up from a Prospective, Randomized Trial of Biolimus A9-eluting Stents with a Biodegradable Polymer vs. Sirolimus-eluting Stents with a Durable Polymer : Final Report of the LEADERS study Presented by P. Serruys at TCT 212 On behalf of the LEADERS investigators 11139--EN

Biolimus-A9 Eluting Stent Biolimus is a semi-synthetic sirolimus analogue with 1x higher lipophilicity and similar potency as sirolimus. Biolimus is immersed at a concentration of 1.6 g/mm into a biodegradable polymer, polylactic acid, and applied solely to the abluminal stent surface by a fully automated process. Biolimus is co-released with polylactic acid and completely desolves into carbon dioxide and water after a 6-9 months period. The stainless steel stent platform has a strut thickness of 12 m with a quadrature link design.

LEADERS all-comers Trial Design Stable and ACS Patients Undergoing PCI Assessor-blind 1:1 Randomisation N=17 Patients 1 European centers BioMatrix Flex () N=8 Cypher Select () N=8 1:3 Randomisation Clinical F/U N=64 Angio F/U N=21 Clinical F/U N=64 Angio F/U N=21 1 o endpoint: MACE: Cardiac death, MI, clinically-indicated TVR (9 mo) 2 o endpoints: Death, CV death, MI, TLR, TVR Stent thrombosis according to ARC Angiographic study: In-stent % diameter stenosis (9 mo) Late loss, binary restenosis DAPT recommended for 12 months

Patient Demographics 87 Patients 8 Patients Age in years 6 11 6 11 Male gender 7% 7% Arterial hypertension 74% 73% Diabetes mellitus 26% 23% - insulin-dependent 1% 9% Hypercholesterolemia 6% 68% Family history of CAD 4% 44% Smoking 24% 2% Previous MI 32% 33% Previous PCI 36% 37% - with drug-eluting stent 12% 14% Previous CABG 11% 13%

Patient Characteristics 87 Patients 8 Patients Chronic stable angina 4% 44% Acute coronary syndrome % 6% Unstable angina 22% 21% Non-ST-elevation MI 17% 18% ST-elevation MI 16% 17% Left ventricular ejection fraction 6 11% 12% Number of lesions per patient 1..7 1.4.7 Lesions per patient 1 lesion 63% 69% 2 lesions 29% 22% 3 lesions 7% 8% > 4 lesions 1% 2% De novo lesions 92% 91% Long lesions (>2 mm) 31% 27% Small vessels (RVD 2.7 mm) 68% 67% Off label use 81% 78%

Patient Flow - Clinical (N=87) (N=839) (N=832) (N=819) (N=838) (N=833) Randomized (N=1,77) 1-year follow-up (N=1,669; 97.8%) 2-year follow-up (N=1,67; 97.1%) 3-year follow-up (N=1,636; 9.8%) 4-year follow-up (N=1,663; 97.4%) -year follow-up (N=1,647; 96.%) (N=8) (N=83) (N=82) (N=817) (N=82) (N=814) 13 Lost to FU 2 lost to FU and alive 11 lost to FU and vital status unknown 11 withdrew 24 Lost to FU 8 lost to FU and alive 16 lost to FU and vital status unknown 12 withdrew

MACE (Cardiac Death, MI and ci-tvr) 3 3 2 2 1 1 1-year RR.88 (.66-1.17) P=.38* 12.1% Δ 1.4 1.7% 2-year RR.83 (.64-1.7) P=.1* 1.% Δ 2. 13.% 3-year RR.8 (.63-1.1) P=.6* 19.3% Δ 3.7 1.6% 4-year RR.82 (.66-1.1) P=.9* 23.1% Δ 3.9 19.2% -year RR.83 (.69-1.2) P=.71* 26.1 % Δ 3.8 22.3 % P non-inferiority <.1 6 12 18 24 3 36 42 48 4 6 Number at risk 8 774 738 718 71 676 6 64 616 89 72 87 78 749 733 723 71 697 67 67 63 618 MACE = cardiac death, MI, or clinically-indicated TVR

Cardiac Death 3 3 1-year RR.78 (.42-1.44) P=.42* 2-year RR.79 (.48-1.31) P=.36* 3-year RR.81 (.2-1.26) P=.34* 4-year RR.88 (.6-1.29) P=.2* -year RR.94 (.67-1.32) P=.72* 2 2 1 1 8.4 % 2.7% 4.%.2% 6.9% Δ.8 Δ.4 Δ.8 Δ 1. Δ.6 8. % 2.1% 3.2% 4.2% 6.1% 6 12 18 24 3 36 42 48 4 6 Number at risk 8 829 814 82 793 776 767 73 742 721 74 87 832 817 86 81 794 788 77 76 744 731

MI 3 3 1-year RR 1.27 (.84-1.94) P=.26* 2-year RR 1.12 (.76-1.6) P=.7* 3-year RR 1.1 (.71-1.44) P=.97* 4-year RR.97 (.7-1.3) P=.87* -year RR.96 (.71-1.3) P=.79* 2 2 1 1 1.% 7.3% 9.%.9% 6.4% Δ 1.3 Δ.7 Δ.1 Δ.4 Δ.6 4.6%.7% 7.2% 8.6% 6 12 18 24 3 36 42 48 4 6 9.9 % Number at risk 8 796 781 767 72 733 717 7 684 661 644 87 791 779 768 761 72 744 721 71 687 67

QW-MI 6 1-year RR.6 (.17-1.93) P=.36* 2-year RR.49 (.1-1.64) P=.2* 3-year RR.39 (.12-1.26) P=.12* 4-year RR.3 (.21-1.32) P=.17* -year RR.49 (.21-1.14) P=.98* 4 3 2 1 2.% 1.% 1.2% 1.6%.8% Δ.3 Δ. Δ.7 Δ 1. Δ.7.%.%.%.9% 1.% 6 12 18 24 3 36 42 48 4 6 Number at risk 8 823 88 79 786 768 79 74 733 71 694 87 828 813 82 798 791 786 767 76 739 726

Non QW-MI 2 2 1-year RR 1.38 (.89-2.16) P=.1* 2-year RR 1.2 (.8-1.82) P=.38* 3-year RR 1.4 (.72-1.2) P=.82* 4-year RR 1. (.71-1.42) P=.98* -year RR.99 (.71-1.37) P=.9* 1 1.4% Δ 1. 3.9% 4.9%.9% 6.8% Δ 1. Δ.4 6.4% 9. % 7.8% Δ.1 Δ.1 7.7% 8.9 % 6 12 18 24 3 36 42 48 4 6 Number at risk 8 82 787 774 79 739 724 77 692 67 63 87 79 783 772 764 7 746 724 714 692 68

Clinically-Indicated TVR 3 3 2 2 1 1 1-year RR.82 (.6-1.19) P=.3* 7.2% Δ 1.2 6.% 2-year RR.8 (.61-1.19) P=.34* 3-year RR.8 (.9-1.8) P=.1* 8.9% 11.% Δ 1.2 Δ 2.2 7.7% 4-year RR.81 (.62-1.7) P=.14* 13.6% Δ 2.4 9.3% 11.2% -year RR.81 (.63-1.) P=.12* 1.% Δ 2.7 12.8% 6 12 18 24 3 36 42 48 4 6 Number at risk 8 797 76 741 727 74 68 668 646 62 6 87 89 776 78 748 736 72 76 687 668 6

Patient Oriented Composite Endpoint (All-cause Death, Any MI, All Revascularization) 4 4 3 3 2 2 1 1 Number at risk 1-year RR.84 (.68-1.4) P=.11* 21.6% Δ 3.1 18.% 2-year RR.83 (.68-1.) P=.* 26.4% Δ 4.1 22.3% 3-year RR.81 (.68-.96) P=.18* 31.8% Δ.4 26.4% 4-year RR.83 (.7-.98) P=.24* 36.2% Δ.2 31.% 6 12 18 24 3 36 42 48 4 6 8 729 661 637 618 94 69 1 3 4 491 87 749 689 672 64 639 619 97 79 7 4 POCE = all death, MI, any revascularization (includes adjudicated and non-adjudicated events) -year RR.84 (.71-.98) P=.24* 4.4% Δ.3 3.1%

Definite Stent Thrombosis (ARC) 6 4 3 2 1 1-year RR.99 (.1-1.94) P=.98* 2.% Δ. 2-year RR.9 (.48-1.67) P=.73* 2.% 2.2% 3-year RR.78 (.43-1.43) P=.43* 4-year RR.62 (.3-1.8) P=.9* 4.% 2.% 2.9% Δ.3 Δ.7 Δ 1.6 2.2% 2.4% -year RR.6 (.3-1.2) P=.6* 4. % Δ 1.9 2.6 % 6 12 18 24 3 36 42 48 4 6 Number at risk 8 816 81 787 776 79 749 732 717 696 678 87 819 84 792 787 78 77 77 747 73 717

Effect of DAPT Discontinuation. Overall Population P =.62* Patient who d/c DAPT 4. 4.2 P =.3* P =.2* 4. 3. 3.6 3. 2. 2.6 2. 1.8 1. 1... N=22/87 N=36/8 Y definite ST in LEADERS..3 N=/16 N=6/16 definite ST after DAPT d/c < 12 M N=2/9 N=1/72 definite ST after DAPT d/c > 12 M (Fisher Exact Test)

Definite ST (ARC) Landmark Analysis @ 1 Year 6 to 1 year RR.99 (.1-1.9) P=.98* 1 to year RR.26 (.1-.68) P=.3* 4 % 3 2. % 2 2. % 2. % 1.66 % 6 12 18 24 3 36 42 48 4 6 P for interaction=.22

Cumulative incidence events (%) Cumulative incidence events (%) Cumulative incid 1 1 able) or triangle (possible) d: d: MACE 1 1 2 Days Days since since Procedure Durable/Biodegradable Polymer DES Clinical Events (Corrected KM Estimates) Linked to to ARC Definition ST Definite ST Probable ST Cardiac Death/MI/Ci TVR Cardiac Death/MI Leaders Y: Leaders Y: Event incidence Possible curves, showing events ST related to ST as circle Cardiac (definite), square (probable) Death or triangle (possible) Event incidence curves, showing events related to ST as circle (definite), square (probable) or triangle (possible) 1 2 3 Censoring 4 events related 6 to definite, 7 probable 8 and 9 possible 1 ST 11 12 13 14 1 16 17 18 19 Censoring 2 events related to definite, probable and possible ST Black: Cardiac Death, Green: Composite of Cardiac Death and MI, Red: MACE Black: Cardiac Death, Green: Composite of Cardiac Death and MI, Red: MACE Days since Procedure 3 3 1 2 3 4 6 7 8 9 1 11 12 13 14 1 16 17 18 19 2 3 4 6 7 8 9 1 11 12 13 14 1 16 17 18 19 2 Durable Polymer () 3 6 7 8 9 1 11 12 13 Days 14 since 1 Procedure 16 17 18 19 2 3 Days since Procedure 6 6 7 7 8 8 9 9 1 1 11 11 12 12 13 13 14 14 1 1 16 16 17 17 18 18 19 19 2 2 Days since Procedure 4 6 Days Days since since Procedure 7 8 9 1 11 12 13 14 1 16 17 18 19 2 2 2 Days since Procedure 6 6 7 7 8 8 9 9 1 1 11 11 12 12 13 13 14 14 1 1 16 16 17 17 18 18 19 19 2 2 Δ 8.2% 2 Biodegradable Polymer () Δ 6.4% 1 1 1 Δ 8.7% 1 Δ 7.4% Δ 6.4% Δ 6.% 14 1 16 17 18 19 2 1 2 3 4 6 7 8 9 1 11 12 13 14 1 16 17 18 19 2 Days since Procedure 1 2 3 4 6 7 8 9 1 11 12 13 14 1 16 17 18 19 2 Days since Procedure

Stratified Analysis of MACE @ Years Favors Favors RR (9%CI) P* Overall 186 (21.7) 216 (2.4).83 (.68 to 1.2).69 Diabetes mellitus ACS Yes 66 (29.6) 6 (29.3) 1.2 (.71 to 1.46).91 No 94 (14.8) 136 (2.6).7 (.4 to.91).7 P for interaction Yes 83 (17.7) 16 (22.4).77 (.8 to 1.3).73 No 77 (19.9) 86 (22.8).86 (.63 to 1.18).3 ST elevation MI.4 Yes 1 (11.1) 32 (22.9).4 (.24 to.83).9 No 14 (2.1) 16 (22.).89 (.71 to 1.11).29 Left anterior descending ns Yes 74 (18.2) 8 (2.4).88 (.64 to 1.2).41 No 86 (19.2) 17 (24.8).76 (.7 to 1.1).6 Multivessel disease ns Yes 44 (21.1) 48 (27.3).7 (.49 to 1.13).16 No 116 (17.9) 144 (21.4).83 (.6 to 1.6).13 De-novo lesions ns Yes 139 (17.6) 16 (21.3).81 (.6 to 1.2).76 No 21 (3.9) 27 (36.).82 (.46 to 1.4).49 Small-vessel disease ns Yes 12 (2.) 131 (23.1).88 (.69 to 1.13).33 No 4 (14.8) 61 (21.8).6 (.44 to.97).33 Long lesions ns Yes 4 (2.6) 63 (28.).72 (. to 1.3).71 No 16 (17.8) 129 (2.7).8 (.66 to 1.1).21 ns ns.2. 1 2 4 All p-values are for superiority * Mantel Cox p-value

Conclusions Biodegradable polymer maintained non-inferiority and improved long-term clinical outcomes compared to through years (P sup =.71) Biodegradable polymer demonstrated a 74% relative risk reduction in very late definite stent thrombosis (VLST) The benefit of biodegradable polymer emerged in the very late phase and was mainly driven by a lower risk of MACE associated with definite VLST

Biolimus A9, BA9, and BioMatrix Flex are trademarks or registered trademarks of Biosensors International group, Ltd. All cited trademarks are the property of their respective owners Not available for sale in the United States and certain other countries. 212 Biosensors International Group, Ltd. All rights reserved

All Death 3 3 2 2 1 1 1-year RR.96 (.6-1.62) P=.87* 3.3% Δ.1 3.2% 2-year RR.88 (.8-1.3) P=.7* 3-year RR.8 (.6-1.16) P=.24*.3% 7.6% Δ 1.4 Δ.6 4.7% 6.2% 4-year RR.9 (.66-1.21) P=.48* 1.4% Δ 1. 9.4% -year RR.88 (.67-1.1) P=.34* 13.7 % Δ 1.6 12.1 % 6 12 18 24 3 36 42 48 4 6 Number at risk 8 829 814 82 793 776 767 73 742 721 74 87 832 817 86 81 794 788 77 76 744 731

MACE @ Years Separated by Median SYNTAX Score (12) The SYNTAX score has no significant effect on the primary endpoint between and 3 3 2 2 1 Syntax Score 12 Syntax Score 12 Syntax Score <12 Syntax Score <12 Low Syntax (< 12) vs. -year RR.86 (.6-1.24) P=.43* High Syntax ( 12) vs. -year RR.71 (.3-.9) P=.21* 3.3% 22.7% 18.8% 16.4% 1 Interaction p-value:.4 Log rank (low vs. high) p-value: <.1 6 12 18 24 3 36 42 48 4 6

Definite ST @ Years Separated by Median SYNTAX Score (12) is associated with a significant reduction in definite ST in the higher SYNTAX scores 1 8 6 Syntax Score 12 Syntax Score 12 Syntax Score <12 Syntax Score <12 Low Syntax (< 12) vs -year RR 1.37 (.47-3.93) P=.6* High Syntax ( 12) vs -year RR.38 (.18-.83) P=.1* 6.6% 4 2 Interaction p-value:.6 Log rank (high vs. low) p-value:.18 1 1 2 2 3 3 4 4 6 2.% 2.4% 1.9%