Avian Influenza: the moving target



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Avian Influenza: the moving target Ilaria Capua 1 & Dennis J Alexander 2 OIE/FAO Reference Laboratories 1 Istituto Zooprofilattico Sperimentale delle Venezie Legnaro, Padova IT 2 VLA Weybridge, UK

H5N1 epidemic-a disease of global H5N1 has become endemic in poultry in several parts of the world It is capable of infecting a variety of birds (@50 species) and 10 species of mammals For every human that is infected, at least 1 million animals are infected relevance

Challenge of the moving target Controlling AI is an enormous challenge for the veterinary community and local administrations First time in history that such a panzootic occurs, the ecology and epidemiology of AI are in constant evolution as it spreads to new ecosystems and hosts Significant funds and resources must be allocated and used in an organised manner heading in the same direction Ongoing evaluation must feed back into adjustment strategies

Atypical characteristics of the H5N1 epidemic Involvement of waterfowl Spill-over to wild birds Billions of susceptible birds in certain affected areas Peculiarity of husbandry/social practices Infection of mammals Human health implications

Understanding AI epidemiology in birds 1. Birds raised for food (industrial, freerange, rural, scavenging) 2. Pet/hobby birds 3. Wild birds

H5N1: unprecedented eco/epidemiological situation Other species Wild bird reservoir Industrial poultry Rural poultry Live bird markets Poultry reared in the open

Photo credit: Fabio Frisca (FAO Vietnam)

Outbreak of Exotic Newcastle Disease in Game Fowl in Southern California - 2002 Fighting cocks have also been responsible for the spread of H5N1 virus

Chicken carcases placed over pond on catfish farm in Indonesia

Catfish feeding on chicken carcass

Ducks on the same catfish farm

TWO EARLY INCURSIONS INTO EUROPE (2004-2005) TO BELGIUM AND GREAT BRITAIN both were contained without spread

Two mountain hawk eagles smuggled by a man travelling from Bangkok to Brussels in November 2004 were found to be infected with HPAI H5N1 virus genetically close to Thailand strains.

HPAI H5N1 in Europe Isolation of HPAI H5N1 from captive cage birds in quarantine in England Virus closest genetically to a 2005 Chinese duck isolate

Spread from East Asia -2006

Wild birds

It is the first time in recorded history that HPAI spills over in a significant manner to the wild bird population

Significant isolations of H5N1 from wild birds in E. Asia Hong Kong 2002 numerous species of wild birds - Ellis et al 2004 Japan 2004 - crows, Mase et al 2005 Mongolia 2005 - migratory waterfowl China 2005 - migratory waterfowl Lake Qinghai - Chen et al., 2005; Liu et al., 2005 Open debate on the carrier state for HPAI

Mute swans seem to have been responsible for spread of H5N1 in Europe in early 2006, most probably due to an unusually cold winter European countries HPAI H5N1 reported in swans January April 2006 Croatia, Germany, Italy, Austria, Bosnia, Greece, Bulgaria, Slovenia, Hungary, Russia, Poland, Serbia, Sweden, UK [Whooper swan] and Azerbaijan, Kazakhstan & Iran

At the moment it is unknown how HPAI will behave in the wild bird population Does it kill all the birds it infects? If so, what are the incubation and shedding periods? If not, for how long can it persist? Is there a true reservoir species?

The primary means of spread is through trade of infected poultry

UK and Hungarian 2007 viruses : 99.6% homology

Spanish influenza 1918-1919 20-40 million dead

Pandemic potential Direct infection of humans with AI Generation of a new pandemic virus through genetic reassortment between an avian and mammalian virus

Human pandemic viruses All the viruses of the 20th century have an avian component It is 28 years [or some would argue 37 years] since the last pandemic, so time is ripe for a new human pandemic All human pandemic viruses known to date are H1, H2 or H3 subtype

Most certainly The next human pandemic virus will have an avian progenitor or component The more AI viruses circulate in birds, the greater the risk of igniting the new pandemic H5N1 HPAI will be extremely difficult to eradicate from developing countries, in which it also represents a serious threat for food security

Avian Influenza - conclusions Is - first of all a disease of animals Control in the animal reservoir is a prerequisite to the management of the pandemic potential The comparison between strains is essential to increase knowledge on AI epidemiology and human health implications

Veterinary virologists lead by example GISAID- Global Initiative on Sharing Avian Influenza Data www.gisaid.org Nature Correspondence 24 Aug, 2006 Signatories: 70 leading scientists, 6 Nobel Laureates Open access, free database which will contain human and animal influenza virus sequences and protects intellectual property

Veterinary initiatives promoting genetic data sharing to support the global H5N1 crisis OIE/FAO: support and promote data sharing OFFLU network collects strains and sequences and promotes deposit in open access databases EPIZONE: EU Network of Excellence, WP 6.2 aims at understanding AI epidemiology through the generation and analysis of sequence data