-86 C ULTRALOW TEMPERATURE FREEZERS. NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 U.S.A. 763.553.1270 www.nuaire.com



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-86 C ULTRALOW TEMPERATURE FREEZERS NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 U.S.A. 763.553.1270 www.nuaire.com

NuAire Glacier -86 C Ultra-low Temperature Freezer NuAire Glacier -86 C Ultra-low Temperature Freezers are developed for reliable storage conditions of research samples for laboratories, hospitals, repositories and more. Through research and development, a new focus has been placed on Glacier s refrigeration system, controls, alarms/monitoring, ergonomics, construction and energy efficient operation to make Glacier Freezers the reliable solution for long-term storage of cancer cells, stem cells, cord blood, T-cells, organ/tissue and other samples. 1

Standard Features Temperature Uniformity Reliable Storage Conditions Environmentally Friendly A cascade refrigeration system that utilizes dual compressors offers a quicker, more reliable way to control storage conditions. - Quicker temperature recovery after door openings - Insulated inner doors help maintain interior temperature - Multi-point gasket seal system ensures a tight seal - A voltage booster option and surge protection offers dependable operation over a wider range of voltages. Properly designed compressors located within the refrigeration system offer a dependable freezer. - Dual compressors run high and low stage refrigerants to maintain temperature uniformity. - Voltage boosters and surge protection work to maintain proper operation. - Dependable NuAire construction and service networks provide a NuAire quality product. Our commitment is to reduce energy consumption, reduce environmental impact and minimize both pollution and the depletion of natural resources. - CFC Free Refrigerants - RoHS Compliant - Low noise operation down to 47 dba - Designed to support LEED certification - Minimal heat output 2

Features [1] Easy-In/Easy-Out door latch for one-handed operation, positive seal against gasket. Padlock provision standard. [2] Universal keyed door lock offers added security. [3] Integrated, microprocessor-based control system and LED display includes comprehensive set point, alarm, monitoring, diagnostic and communications functions (details on next page). [4] Circular-chart temperature recorder (optional) mounts easily in pre-engineered mounting space. * [5] Insulated and gasketed inner doors seal inside to offer additional protection and improve uniformity. Inner door latches are standard. Doors can be easily removed for defrosting. [6] Front access to washable, electrostatic condenser filter for routine condenser air filter cleaning. [7] High impact, recessed casters and leveling feet.* [8] New generation ArticGard compressors are specifically designed for low temperature applications.* [9] Multiple access ports permit insertion of independent probes, instrumentation, liquid N2 or liquid CO2 back-up injectors.* [10] Commercially available HFC refrigerants are highly efficient, environmentally safe and non-ozone depleting.* [11] Internal voltage and power management systems assure component protection over wide voltage ranges.* [12] Remote alarm contacts, optional communication port available. [13] Vacuum relief valve. [13] [12] [2] [6] [1] 3 * not visible [4]

TEMPERATURE(C) BATTERY FILTER ALARM ALARM BUZZER TEST SET Construction Cabinet Insulation Minimum 5 (12.7 cm) CFC-free foamed-in-place urethane, closed-cell, for thermal protection and structural strength Aluminum Tape Increases evaporator surface Access Ports One port on back side for CO 2 back-up, One port on bottom for recording (17mm diameter) Door Handle / Latch Rugged, one-handed operation, fastener for padlock Evaporator Tube on sheet type Gasket Multi-point seal Remote Alarm Dry Contacts Power switch, battery back-up alarm Refrigeration System Custom engineered compressors, high performance lubricants Low Profile Upright design Inventory Racks Optional, stainless steel with fiberboard boxes and dividers Heated Door Seals Hot gas minimizes frost build-up around door gasket Control Center Microprocessor control at eye level Outer Door Minimum 3 (7.6 cm) high strength foamed-in-place urethane insulation Insulated Inner Doors High impact plastic with recessed handle Condenser Fan Durable fan with aerodynamically designed fan blades CFC-Free Refrigerants High stage R407D (HFC), Low stage R508 (HFC) Air Cooled Condenser Large surface area, copper tube, aluminum fins Recorder Optional Cobex system with mounting plate Washable Pre-filter Protects condenser fins against dust build-up Heavy Duty Casters For mobility as required Barrier Film Rigid Foamed Polyurethane Vacuum Insulation Panel Interior Exterior Model NU-9728 features a high-efficiency Vacuum Insulation Panel thin-wall composite design that yields more interior storage volume within a conventional freezer footprint. Vacuum Insulation Panels minimize energy transfer to and from the ultra-low temperature interior. The composite construction, complete with reflective barrier film and structural closed-cell foam, is used on all walls and the outer door. This advanced insulation technology offers structural stability to eliminate distortion, and inhibits moisture accumulation that can lead to icing. Aggregate insulation efficiency minimizes compressor cycle run-time to lower energy costs. 4

Simplified Controls [1] [3] [5] [4] [10] [9] [8] [2] [6] [11] [7] [1] Temperature Digital display defaults to actual chamber temperature. Display mode changes when set point, alarm parameters, programming and diagnostic functions are performed. [2] Status Light Status alert function uses predictive intelligence to determine if freezer is operating within specifications under existing environmental conditions. [3] Battery Light Ni-MH battery powers control memory and alarm functions during power failure. [4] Alarm Light Alarm indicator lamp glows when freezer is in alarm condition; alarm ring back is factory set for 30 minutes, adjustable in 10 minute increments from 10 to 60 minutes. [5] Filter Light Filter indicator lamp glows when electrostatic filter requires removal for cleaning. [6] Door Light Door alarm indicator has 2 minute delay until audible alarm activates; delay time is adjustable. [7] Right Arrow Set point entry advances digital display to next position. [8] Up Arrow Set point entry advances digital to next value from 0 to 9. [9] Set Button Press to set temperature; set button is also used for other diagnostic functions. [10] Alarm Test Button Alarm test verifies readiness of alarm function and Ni-MH battery charge. [11] Buzzer Button Buzzer silence temporarily mutes alarm. [12] Compressor start-up delay sequence delays re-start after building power failure; allows facility power to reach equilibrium to permit smooth compressor start-up. 5

Alarm Systems Alarms Alarm Type Event Visual Alarm Audible Alarm Signal to Alarm Contact Status Alert Abnormal ambient Temperature (too high or too low), or abnormal freezer loading (too much warm product at once) Flashing STATUS Indication Light None None High Temperature Interior chamber cools beyond high Periodic Beep. A 15 minute Yes temp set point Flashing ALARM delay after programming Indication Light; Low Temperature Interior chamber warms beyond low the set point avoids a Yes LED Display Flashes temp set point premature event. Actual Chamber Temp. Power Failure Loss of Power Periodic Beep Yes System Monitoring Failure Sensor Abnormality Error Code Solid Beep No Status Alert Freezer Running Under Stress Low Voltage High Ambient Temperature Lamp Illuminated None No Auto Return Touch key is not pressed for 90 seconds None No Door Alarm Door Ajar OPEN lamp on Solid Beep Yes 6

Energy Saver Cascade Cooling System One of the most important concepts in designing a superior energy saving ultra-low freezer is how efficiently heat is exchanged between the high and low stage circuits. By providing optimum heat exchange pathways in the design, it not only increases efficiency of the system, leading to greater energy savings, but it will also have an effect of reducing stress on the compressors, leading to greater overall system reliability. The new cap tube heat exchanger is but the latest step in increasing the available heat exchange areas in the system. This patent pending innovation drastically increases the efficiency of the entire system. The end result is less energy consumption, while improving the overall efficiency of the freezer. 7

Cascade Cooling System A B C D E F G H I Low Stage Capillary Tub Liquid refrigerant under pressure is passed through the capillary tube where it evaporates in the low stage evaporator to absorb heat energy from the product stored in the freezer. Evaporator Coil The evaporator coil is wrapped around the exterior shell to provide proper temperature uniformity within vacuum insulation panels and foamed-in-place urethane insulation. Capillary Tube Low stage capillary tube heat exchanger provides heat transfer between high and low temperature points offering better energy efficiency. Low Stage Refrigerant Commonly available worldwide, R508 Low Stage Heat Exchange Heat is efficiently transferred from the low stage to the high stage. Low Stage Compressor The compressor pumps refrigerant through the low stage circuit. Air Cooled Pre-Condenser Removes heat energy from the high stage refrigerant en route to the low Istage compressor. Low Stage Oil Heat Exchange High stage refrigerant passes through the low-stage oil pump to cool oil resulting in high-stage refrigerant being used to increase the durability of the low stage compressor. Inter Stage Heat Exchanger To increase overall system efficiency heat energy is transferred to the high stage circuit. J K L M N O P High Stage Oil Heat Exchange High stage refrigerant passes through the high stage oil to cool lubricating oil en route to the low stage compressor through the air-cooled pre-condenser. Thereby increasing the reliability of the high stage compressor. High Stage Capillary Tube Liquid refrigerant under pressure is passed through the capillary tube where it evaporates in the inter stage heat exchanger to absorb heat energy from the low stage refrigerant circuit. Main Condenser and Motor/Fan An exclusive triple pass forced air condenser increases overall system efficiency by providing maximum surface area for heat rejection. High Stage Refrigerant Commonly available worldwide. Selected for optimum cooling performance in compliance with international environmental protection laws. Air Cooled Pre-Condenser Removes heat energy from the high stage refrigerant en route to the high stage oil reservoir. High Stage Compressor The compressor pumps refrigerant through the high stage circuit. Instrumentation (Not Shown) Temperature and pressure sensors throughout the high and low stage circuits transmit information to the Status Alert central controller for operation, monitoring, interpretation and component protection. E B D C F H A G I K L N O K M High Stage Circuit Low Stage Circuit 8

Options and Accessories CO 2 Back-Up System Will maintain -70 C at a 45 Kg. / Hr. rate. Surge Suppressor Tough, Safe Metal Housing 2 or 3 Fiberboard Boxes Cryo Safety Gloves Protection for Hands and Arms Temperature Recorder Easy to Install 9

Model NU-9483 NU-9668 NU-9728 Rack Capacity 16 Racks 24 Racks 24 Racks Adjustable Freezer Rack for 2 or 3 Boxes Part # UFMC-45-2-3 UFMC-45-2-3 UFMC-46-2-3 2 Box Capacity 320 (4 deep, 5 high) 480 (4 deep, 5 high) 576 (4 deep, 6 high) 3 Box Capacity 192 (4 deep, 3 high) 288 (4 deep, 3 high) 384 (4 deep, 4 high) Prepackaged Freezer Rack with 2 Boxes Part # SV-HL-20-2-81 SV-HL-20-2-81 SV-HL-20-2-81 2 Box Capacity 320 (4 deep, 5 high) 480 (4 deep, 5 high) 480 (4 deep, 5 high) Maximum Capacity Model NU-9483 NU-9668 NU-9728 Configuration / Rack Positions 4 Columns / 4 Rows 6 Columns / 4 Rows 6 Columns / 4 Rows # of 2 (51 mm) 2 ml Fiberboard Boxes in Racks 384 480 576 # of 2 ml Sample Vials, 2 (51 mm) Box, 100 Cell Dividers 32,000 48,000 57,600 # of 3 (76 mm) 4 ml Fiberboard Boxes in Racks 192 336 384 # of 4 ml Sample Vials, 3 (76 mm) Box, 100 Cell Dividers 19,200 33,600 38,400 # of Standard Microplates, Foil Cover in Racks 2,016 3,024 3,456 # of Standard Microplates, Lid Cover in Racks 1,632 2,448 2,596 10

NU-9000 Series -86 C Ultralow Temperature Freezers Specifications Model Interior Volume Footprint Interior Dimensions (W x D x H) Exterior Dimensions (W x D x H) Weight Temperature Range Electrical* Warranty NU-9483 17.1 ft 3 487 L 8.37 ft 2 24.8 x 23.6 x 50.4 in. 0.78 m 2 (630 x 600 x 1280 mm) 35.0 x 34.4 x 78.3 In. (890 x 870 x 1990 mm) 706 lbs (320 kg) -50 C to -86 C GA: 115V / 60Hz E: 230V / 50Hz 2 Year Parts & Labor (US & CA) 4 Year Parts (Global) NU-9668 23.5 ft 3 668 L 10.63 ft 2 34.2 x 23.6 x 50.4 in 0.99 m 2 (870 x 600 x 1280 mm) 44.5 x 34.4 x 78.3 in. (1130 x 867 x 1990 mm) 794 lbs (360kg) -50 C to -86 C E: 230V / 50Hz 2 Year Parts & Labor (US & CA) 4 Year Parts (Global) NU-9728 25.7 ft 3 728 L 9.51 ft 2 34.2 x 23.6 x 55.1 in. 0.88 m 2 (870 x 600 x 1400 mm) 39.8 x 34.4 x 78.3 in. (1010 x 870 x 1990 mm) 820 lbs (373 kg) -50 C to -86 C GA: 115V / 60Hz 2 Year Parts & Labor (US & CA) 4 Year Parts (Global) *Specify model with appropriate letter suffix for electrical specifications. NU-9728GA for 115 Vac / 60 Hz 11

Operations and Specifications Model NU-9483 NU-9668 NU-9728 High Stage 1100 W 1100 W 750 W 1 HP Low Stage 1100 W 1100 W 1100 W 1 1/2 HP Voltage Booster GC Models Only GC Models Only No Noise Level Standard ( 49 dba) Standard ( 49 dba) Standard ( 49 dba) Shelf Access Port Stainless steel, adjustable 3 shelves (23.9 w x 21 d) Max Load 110 lbs (50kg) / shelf Stainless steel, adjustable 3 shelves (848 mm w x 533 mm d) Max Load 110 lbs (50kg) / shelf 0.066 (17 mm) diameter / 2 locations (back/bottom left) Refrigerants HFC Refrigerants High Stage; R-404A / Low Stage; R-508 HFC Refrigerants High Stage; R-407D / Low Stage; R-508 HFC Refrigerants High Stage; R-404A / Low Stage; R-508B Alarm Remote Alarm Contact High/low temp, Power failure, Filter check, Batter check High temp. alarm, Low temp. alarm, Power failure alarm, Door alarm, Filter alarm Accessories 1 set keys, 1 scraper 1 set of key, 1 scraper, 1 stick for air intake port cleaner Inner Door Configuration Two (2) Seismic Brackets Leveling Feet Insulation Temperature Controller Temperature Display Standard; hard connect to facility wall Standard Rigid polyurethane foamed-in place Microcomputer control system Digital Vacuum insulation panel; Rigid polyurethane foamed-in place Thermal Sensor Platinum resistance (Pt 1000Ω) Battery Nickel-metal-hydride battery, DC 6 V, 1100 mah, Auto-recharge (5HR-AAC) 12

Sales or Service Representative To Locate a NuAire Sales or Service Representative Please visit NuAire s Web site at: www.nuaire.com and select the Sales Team menu item. Information can be found there for both United States and international distributors. 13

For your Laboratory NuAire s Family of Products energy saver CO 2 Incubators Biological Safety Cabinets Laminar Airflow Products High Speed, Ultra, and Micro-Ultracentrifuges 14

Your Local NuAire Partner NuAire, Inc. 2100 Fernbrook Lane Plymouth, MN 55447 U.S.A. For more information please visit www.nuaire.com or call 1.800.328.3352 Copyright. NuAire, Inc. All Rights Reserved. 20-1161-DB-EN-V5-201502 Make your request online