DIRECTORATE of LABORATORY MEDICINE

Transcription

1 Blood Tube Guide April 2012 DLM Client Services /4428 Clinical Biochemistry Lab Specialist Lab Duty Biochemist; Bleep 4375 Paediatric Core Laboratory Paediatric Duty Biochemist; Haematology Coag, Counts, Red cell Molecular Diagnostics Transfusion Immunology Microbiology /8854/3961 Virology /8854 Other Useful Numbers: Molecular Genetics Laboratory Cytogenetics Transplantation Laboratory You can also visit DLM website at:

2 ABBR EV TEST DEPARTMENT TUBE TOP COLOUR F ADULTS ACTH Biochemistry Green IN ICE CONTACT LAB APTT Activated partial thromboplastin time Haematology Light blue TUBE TOP COLOUR F PAEDIATRIC / NEONATAL Orange 4.0 ml top ALT Alanine transaminase Biochemistry Orange 1.3ml or Albumin Biochemistry Orange 1.3ml or Aldosterone Biochemistry Green urgent to lab ALP Alkaline phosphatase Biochemistry Orange 1.3ml or Alkaline phosphatase isoenzymes Biochemistry or red Orange 1.3ml or AFP Alphafetoprotein Biochemistry Orange 1.3ml or Alpha-1 Antitrypsin Biochemistry Amiodarone Biochemistry Ammonia Biochemistry Purple Red top 1.3ml or EDTA IN ICE. CONTACT LAB*. Amitriptyline Biochemistry Amylase Biochemistry Orange 1.3ml or Amylase isoenzymes Biochemistry or red Orange 1.3ml or Androstenedione Biochemistry ACE Angeotensin converting enzyme Biochemistry Anion-Gap Biochemistry Orange 1.3ml or ENA Antibody to ENA Immunology Anticoagulant Control Haematology Light blue ANA Anti-nuclear antibody Immunology Antioxidant profile Biochemistry CONTACT LAB BEFE COLLECTION Antithrombin Haematology Light blue AP100 AP100 Immunology Red URGENT TO LAB APO-E Apolipoprotein-E genotype Biochemistry Purple genoty pe AST Aspartate transaminase Biochemistry Orange 1.3ml or BCR-ABL Molecular Diagnostics 4 x Purple BHCG (Tumour Marker) Biochemistry Orange 1.3ml or Bicarbonate Biochemistry Orange 1.3ml or Bile acid Biochemistry B12 Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml

3 Bilirubin Biochemistry Orange 1.3ml or Blood count Haematology Purple Blood grouping+antibody Haematology Pink Red Top screen Bone Markers CTX,PINP Biochemistry Purple Ca-125,Ca19-9,Ca15-3,CEA Biochemistry (tumour markers) C3 C3 Immunology C4 C4 Immunology CRP C Reactive protein Biochemistry Orange 1.3ml or Caeruloplasmin Biochemistry White Plastic Plain Clotted 5.0ml, always ordered with copper Calcitonin Biochemistry URGENT TO LAB ON ICE Calcium Biochemistry Orange 1.3ml or Carbamazepine Biochemistry Carbohydrate deficient Biochemistry transferrin CD4 CD4 Immunology Purple Cell Markers Immunology Purple CH100 Immunology Red URGENT TO LAB Chloride Biochemistry Orange 1.3ml or Cholesterol Biochemistry Orange 1.3ml or Cholinesterase Biochemistry Chromagranin A and B Biochemistry Purple Chromosome Analysis Cytogenetics CIH inhibitor Immunology Light blue URGENT TO LAB Clomipramine Biochemistry Coagulation disorders Haematology Light Blue Coagulation screen Haematology Light Blue Cold agglutinins screen Haematology Pink + purple at 37 C By arrangement olny Copper Biochemistry Navy blue White Plastic Plain Clotted 5.0ml, usually ordered with caeruloplasmin unless for nutrition monitoring C -peptide Biochemistry URGENT TO LAB ON ICE Orange 1.3ml or Cord blood investigations Haematology 1 pink (maternal) 1 pink (cord) Cortisol Biochemistry Orange 1.3ml or CRP C-reactive protein Biochemistry Orange 1.3ml or Creatinine Biochemistry Orange 1.3ml or CK Creatine kinase Biochemistry Orange 1.3ml or Cryoglobulins Immunology Red CONTACT LAB ext 6440

4 CTX Crosslaps (bone marker) Biochemistry Purple Cyclosporin Biochemistry Purple Purple top D-dimer Haematology Light blue 11-deoxycortisol Biochemistry KEEP ON ICE URGENT DHEA sulphate Biochemistry DIC screen Haematology Light blue Differential leucocyte count Haematology Purple Digoxin Biochemistry Direct Antibody Test (DAT) Haematology Pink Red Top DNA studies Molecular Genetics Purple Laboratory EDTA Dothiepin Biochemistry Down's Syndrome Screen Biochemistry Purple EDTA U&E Electrolytes Biochemistry Orange 1.3ml or EP Electrophoresis Immunology EOP Erythropotin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml ESR Erythrocyte sedimentation rate Haematology Purple Can be performed from blood count sample FDP Purple/ black top. Can be performed from blood count sample Ethanol Biochemistry Grey or yellow Orange 1.3ml or Ethosuximide Biochemistry Orange 1.3ml or Ethylene glycol Biochemistry or Grey Orange 1.3ml or Factor V Leiden (& Molecular Diagnostics Purple prothrombin) genotyping Factor VIII Haematology Light blue Factor assay (pre/post dose) Haematology Light blue Ferritin Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml Fibrinogen estimation Haematology Light blue Fibrin degradation products Haematology Light blue (D-dimers) FK506 - Tacrolimus Biochemistry Purple Purple top Flecainide Biochemistry Folate (serum only) Haematology Red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml Folate ( RBC) Haematology purple purple FSH Follicle Stim. Hormone Biochemistry Orange 1.3ml or free T4 Biochemistry Orange 1.3ml or FBC Full Blood Count (FBC) Haematology Purple G6PD Haematology Purple GGT Gamma-GT Biochemistry Orange 1.3ml or

5 Genetic leuk Molecular Diagnostics 4 x Purple Gentamicin Biochemistry Red top Orange 1.3ml or GHRH Biochemistry Red GIFN Immunology Special 3 bottles sent with patient Glandular fever Haematology Purple Glucose Biochemistry Grey top 1.0ml GTT Glucose Tolerance Test Biochemistry Grey top 1.0ml HbA1c Glycated Haemoglobin Biochemistry Purple Purple top HFE HCG IGF1 Growth Hormone Biochemistry Gut hormone Biochemistry Bottle from lab KEEP ON ICE URGENT* Haematinics Haematology Red + purple Haematocrit Haematology Purple Haemochromatosis Molecular Diagnostics Light blue or purple genotyping Haemoglobin Haematology Purple Haemoglobin F Haematology Purple Haemoglobin A2 Haematology Purple Haemoglobinopathy Haematology 2 x 5ml Purple purple Purple top on ice HbA1c Biochemistry Purple Purple top Haemolytics Haematology purple purple Haemophilia genetics or Molecular Diagnostics 4 x 5 ml Purple mutation screen Hams test Haematology Purple Haptoglobin Haematology Red Human chorionic Biochemistry gonadotropin Heparin Haematology Light blue HLA B27 Transplantation Lab Purple (EDTA tube) Purple (EDTA tube) HLA B57 typing for Abacavir Transplantation Lab Purple (EDTA tube) Purple (EDTA tube) hypersensitivity HLA antibodies renal recipient Transplantation Lab Red Top tube (Plain). No anticoagulant Red/White Top tube (Plain) No anticoagulant. HLA typing HSCT patient Transplantation Lab Purple (EDTA tube) Purple (EDTA tube) Homocysteine Biochemistry Grey NOTE TIME ON BOTTLE and send immediately to lab. for rapid separation. Hydroxypcortisol (17-OH Biochemistry Purple or yellow cortisol) Imipramine Biochemistry Immunoglobulins: Immunology IgA/IgG/IgM Immunology IgE allergen specific Immunology IgE total Immunology IGF1 ( Insulin like growth factor 1) Orange 1.3ml or Biochemistry Red Orange 1.3ml or

6 IgG/IgM antibody to ds-dna Immunology IgG/IgM anti-cardiolipin Immunology IgG1/IgG2/IgG3/IgG4 Immunology subclass ID Immunodeficiency Immunology Purple Immunofixation Immunology Immunophenotyping Immunology Purple Insulin Biochemistry Red- ON ICE + grey Orange 1.3ml or ON ICE Intrinsic factor antibodies Haematology Red INR INR Haematology Light blue Iron Saturation Biochemistry Orange 1.3ml or Iron Biochemistry Orange 1.3ml or Isoenzymes (Alkaline phosphatase/amylase isoenymes) Biochemistry or red Orange 1.3ml or Molecular Genetics Green Laboratory (lithium/heparin) tube Karotyping (chromosome analysis) KCT Haematology Light Blue Kleihauer test Haematology Pink Lactate Biochemistry Glucose tube (grey) top 1.0ml SEND STRAIGHT TO LABATY Lamotrigine Biochemistry Lead levels Biochemistry Purple Red top 1.3ml PLASTIC EDTA LH Luteinising Hormone Biochemistry Light green Orange 1.3ml or Lipids Biochemistry Orange 1.3ml or Lithium Biochemistry Long chain fatty acids Biochemistry Purple NOTE: this is not the same test as free fatty acids Lupus anticoagulant Haematology 2 x Light blue Lymphocyte subset Immunology Purple Magnesium Biochemistry Orange 1.3ml or Malarial parasites Haematology Purple MCT Mast cell tryptase Immunology NOTE TIME ON BOTTLES AND REQUEST CARD MCH Haematology Purple MCHC Mean cell haemoglobin Haematology Purple concentration MCV Mean cell volume (MCV) Haematology Purple

7 Mercury levels Biochemistry Purple Methotrexate Biochemistry Mitochrondial DNA Molecular Genetics Laboratory Monospot (Paul Bunnell) Haematology Purple Neural tube defect screen Biochemistry Oestradiol Biochemistry Orange 1.3ml or Orosomucoid Biochemistry Orange 1.3ml or Osmolality Biochemistry Orange 1.3ml or PINP PIIINP/ P3NP Procollogen type 1 (bone marker) Biochemistry Procollogen type 3 (liver Biochemistry fibrosis marker) Paul Bunnell test (Monospot) Haematology Purple Paracetamol Biochemistry Orange 1.3ml or PTH Parathyroid hormone Biochemistry Purple Purple top PTHrp PTH related peptide Biochemistry Contact lab for special tube PCR/ viral load Virology Purple EDTA Purple top Phenobarbitone Biochemistry Orange 1.3ml or Phenytoin Biochemistry Orange 1.3ml or Phosphate Biochemistry Orange 1.3ml or Plasma Metadrenalines Biochemistry Purple URGENT TO LAB IMMEDIATELY Plasma osmolality Biochemistry Orange 1.3ml or Plasma renin Biochemistry green sent immediately to lab Plasma viscosity Haematology 4 x purple Platelet aggregation / function Haematology 6 x light blue (By appt) Purple top tube Platelet aggregation/function + Haematology 8 x light blue (By appt) nucleotides Porphyrins Biochemistry Purple top + urine. Contact lab. Before Purple top + urine. Contact lab. Before collection and collection and send send straight to lab. straight to lab. Protecting samples from Protecting samples light. from light. Potassium Biochemistry Orange 1.3ml or Pregnancy testing (blood) Biochemistry Orange 1.3ml or

8 PSA, FPSA QHCG DIRECTATE of LABATY MEDICINE Progesterone Biochemistry Prolactin Biochemistry Orange 1.3ml or Propranolol Biochemistry Prostate Specific Antigen ( Biochemistry Free or Total) Protein S (free) Haematology 2 x Light blue Protein C Haematology 2x Light blue Protein - total Biochemistry Orange 1.3ml or Prothrombin time Haematology Light blue Prothrombin (& Factor V Molecular Diagnostics 1 x Purple Leiden) genotyping Quantiferon Immunology Contact LAB Quantitative HCG (For Blood pregnancy test / ectopic pregnancy) Biochemistry Orange 1.3ml or Renin Biochemistry dark green send immediately to lab (NOT on ice) Reticulocytes count Haematology Purple Can be performed from blood count sample RF Rheumatoid factor Immunology Purple Salicylate Biochemistry Orange 1.3ml or Selenium Pancreatic Laboratory Red White Plastic Plain Clotted 5.0ml Serology bacteria / viral Virology Red Serum and red cell folate Haematology Purple + red Serum Z/1.3 plastic bottle/white plain Clotted 2.0ml and purple top. SHBG Biochemistry Sickle test Haematology Purple Sirolimus Biochemistry Purple Purple top Sodium Biochemistry Orange 1.3ml or FK506 Tacrolimus Biochemistry Purple Purple top Testosterone Biochemistry Thalassaemia Haematology Purple Theophylline Biochemistry Orange 1.3ml or Thrombophilia screening Haematology 2 x Light Blue Thyroglobulins Biochemistry TFT, T3, T4, TSH Thyroid Function Test Biochemistry Orange 1.3ml or TPO Thyroid peroxidase Immunology TIBC Total Iron Binding Capacity Biochemistry Not available for adults, see transferrin saturation Transferrin saturation Biochemistry Orange 1.3ml or

9 Transferrin Biochemistry Triglycerides Biochemistry Orange 1.3ml or TropT Troponin T Biochemistry Tumour markers Biochemistry CA 15-3,CA 19 9,CA 125, CEA Urate Biochemistry Orange 1.3ml or Urea Biochemistry Orange 1.3ml or Uric Acid Biochemistry Orange 1.3ml or Valproic Acid/Valproate Biochemistry Virology Serology Virology Red Virology PCR/viral load Virology Purple Vitamin B12 Haematology Red Vitamin C Biochemistry prior 2 x green to collection to alert lab to collect samples from OPD Vitamin D Biochemistry Vitamin E Biochemistry or red Von Willebrand Haematology 2 x light blue Von Willebrand genetics or Molecular Diagnostics 4 x light blue mutation screen Zinc Biochemistry Navy blue White Plastic Plain Clotted 5.0ml

10 Coagulation Specimen Requirement Guide Coagulation Test COAG Profile DIC Screen (includes Fibrinogen and D-Dimer/FDP) D-DIMER (FDP) HEPARIN Control INR Blue Top 4.5ml Number of Specimens Any combination of these tests can be performed on 1 sample DLE (Lupus Screen) 2 THROMBOPHILIA Screen (includes Anti-thrombin 3 AT111, Protein C, Protein S, Factor V Leiden F5L, Prothrombin Gene Variant PGV) 2 FACT Assays (multiple) 1 Von Willebrand Screen (includes Factor 8 assay F8C, Ristocetin Co Factor RCF, Von Willebrand antigen VWAG. 2 Platelet Aggregation/Nucleotides For any queries contact the Coagulation lab. On ext By appointment only, please check with the coagulation laboratory. Ext