The Effect of Coniferous Tree Soil on Bacterial Growth. Kirstyn Heino and Aleksandra Mijovic. Abstract
|
|
- Amie Bruce
- 7 years ago
- Views:
Transcription
1 The Effect of Coniferous Tree Soil on Bacterial Growth Kirstyn Heino and Aleksandra Mijovic Abstract We tested whether soil in areas where there are coniferous tree populations will have more acidic soil and less bacterial growth than in areas with no coniferous tree growth. We found that there was more bacterial growth in soil taken from deciduous trees compared to that of soil taken around coniferous trees (P= 0.01). Keywords: Soil ph, Bacterial Growth, Coniferous and Deciduous Trees Introduction Soil is a very rich structure composed of many materials that influence the growth of microorganisms and their activity (Angers & Caron, 1998). Any changes to the soil s ph, water quantity and nutrients can effect plant growth and the microorganisms that live in the soil (Angers & Caron, 1998). In particular, soil bacterial communities are influenced greatly by ph (Chamier, 1987). Soil ph is a measure of how acidic or basic the soil is. The ph is determined by how many hydronium ions (H + ) there are in the soil (Skyllberg et al, 2001). ph can range from 0 to 14, with 7 being neutral. Any ph value below 7 is considered to be acidic and any ph value above 7 is considered to be basic. The ph of soil is very important for plant growth because it controls the chemical forms of nutrients in the soil (Skyllberg et al, 2001). In addition, the diversity of bacteria in soil is determined greatly by the ph of soil. Most bacteria will live in soil that has a ph range between 6.5 and 7.5 (Skyllberg et al, 2001). However, bacteria that are acidophiles will live in acidic soils that can range from 2 to 5. Acidophilic bacteria are able to live in these acidic conditions because they are able to continuously transport hydrogen ions out of their cells (Skyllberg et al, 2001). On the other hand, bacteria that require very basic environments are called
2 alkaliphiles. These bacteria will live in soils that have a ph range from 8 to 11. They are able to live in a basic environment because they transport hydrogen ions into their cells (Skyllberg et al, 2001). However, the most common is neutrophilic bacteria. Neutrophilic bacteria will grow in neutral soil, which is around a ph of 7 (Skyllberg et al, 2001). Soil ph is important because it controls how much nutrients, carbon and essential trace elements are available. In addition, bacteria in soil are essential to plant growth in various ways. Bacteria help with many chemical processes, including nutrient transformations and degradation of organic matter (Skyllberg et al, 2001). For that reason, any sudden changes to a specific soil s ph can affect bacterial growth. If the plant relies on bacteria for nutrient absorption, such as nitrogen fixation in leguminous plants, that will directly affect the plants growth. Soil ph is different depending on the type of surrounding vegetation, such as deciduous and coniferous trees. Pine trees are a type of conifer that has needles instead of the leaves and they grow best in acidic soil (Skyllberg et al, 2001). The acidity of soil surrounding pine trees is said to be caused by the pine tree needles that drop to the ground. However, it is caused by the amounts of nutrients that are taken up by the pine tree itself (Skyllberg et al, 2001). When nutrients are taken out of the soil, the soil can become acidic, making it less suitable for neutrophilic or alkaliphilic bacteria to grow. In addition, pine tree needles are slightly acidic, which can make the soil acidic. Skyllberg et al (2001) studied the effects of ph in acidic soils. The authors discovered that in areas where there was coniferous tree growth, such as a temperate forest environment, pine trees grew best in acidic soil (Skyllberg et al, 2001). As the needles decompose, they returned acid to the soil (Skyllberg et al, 2001). As a result, the ph of the soil surrounding coniferous trees decreased, thus becoming more acidic and creating less bacterial growth. They also studied soil nutrients and found that nutrients were lower in areas with acidic soil, which also contributed to why there was less bacterial growth. Factors including the soil s fertility, moisture, temperature, aeration, light and soil organic matter, also contribute to the activity and abundance of soil microorganisms (Skyllberg et al, 2001).
3 However, deciduous trees grow best in neutral soil (Angers & Caron, 1998). When deciduous trees lose their leaves they lose nutrients. Coniferous trees will usually adapt to low nutrient levels in the soil, while deciduous trees cannot. Since deciduous trees grow best in neutral soil, we can infer that there may be more bacterial growth, just because the majority of soil bacteria grow best in neutral soil. Based on this research, we hypothesized that soil surrounding coniferous trees will be more acidic and have less bacterial growth, while soil surrounding deciduous trees will be more basic and have more bacterial growth. If the hypothesis that bacteria growth in coniferous tree soil is less, one would predict it is due to pine needles adding more acid to the soil and causing a lower ph, which may not be optimal conditions for bacteria to grow. Materials and Methods On October 29, 2012, we collected our soil samples at 20 different locations in the Milwaukee Area (Table 1 and 2). We collected 10 pine tree soil samples and 10 non-pine tree soil samples. Samples were taken from horizon A topsoil, of the various sites using a garden shovel and plastic spoons. Samples were placed into Ziploc bags and labeled according to their location. Samples were left outside overnight and then transported to Alverno College in Milwaukee, WI, for testing. To prepare our samples for plating, we gathered two 250 ml beakers to mix our samples, distilled water, ph strips, sterile cotton swabs, and 20 nutrient agar plates that were prepared in advanced, by Alverno College s Microbiology Lab Department. First, using a balance, we weighed out 10 grams of soil and mixed it with 10 ml of distilled water in a beaker to make a slurry. Next, we took the ph strips and recorded the ph of each sample (Table 1 and 2). After the samples were thoroughly mixed, we labeled 20 nutrient agar plates according to soil location and type. Then, we used a plastic 1 ml pipette to drop four drops of slurry onto the plates. We used a sterile cotton swab to gently streak the plates so that the agar was lightly covered with the soil slurry. We placed the lid of the Petri plate ajar to let the samples dry before inverting them for incubation. Each of the samples were prepared following these steps.
4 After all the plates were completely dried, we inverted them and placed them into a 35 C incubator for 24 hours before results were read and recorded. After 24 hours the plates were placed into a cold room for 24 hours before results were read. This was done to stop the growing process. The bacteria were growing rapidly and we did not want confluent growth on the plates. Bacterial counts were determined by counting the number of colonies present in a quarter of the plate and multiplying that number by four. We eliminated soil clumps on the plates or areas that were not covered by the sample slurry. The counts from each sample group were averaged, and then the bacterial plates were autoclaved and discarded. Data was analyzed using a 1 tailed, type 2 dependent T-Test on Excel for Windows Results Bacterial growth on agar plates with non-pine tree soil was significantly greater than bacterial growth on agar plates with pine tree soil (P=0.01). The mean of the pine tree soil bacterial colonies was with a standard deviation of The mean of the non-pine tree soil bacterial colonies was with a standard deviation of The mean of the pine tree soil ph was 8.5 with a standard deviation of The mean of the non-pine tree soil ph was 5.8 with a standard deviation of
5 Table 1. Record of pine tree soil samples location, ph, and average number of bacterial colonies found on plates Soil Sample Location of Sample ph of soil Number of Bacterial Pine sample Colonies Boerner Drive Hales Corners, WI Boerner Drive Hales Corners, WI Boerner Drive Hales Corners, WI South 92 nd street, Hales Corners, WI South 92 nd street, Hales Corners, WI Seminary Woods, St. Francis WI Seminary Woods, St. Francis, WI Lenox, Milwaukee, WI backyard Lenox, Milwaukee, WI backyard Crawford, St. Francis, WI, backyard (Blue Spruce) Table 2. Record of non-pine tree soil samples location, ph, and average number of bacterial colonies found on plates. Soil Sample Pine Location of Sample ph of soil sample Number of Bacterial Colonies 1 Twelve Bridges Condominiums (backyard), Greenfield WI (Oak) 2 Twelve Bridges Condominiums (backyard), Greenfield WI (Oak) 3 Twelve Bridges Condominiums (backyard), Greenfield, WI (oak) 4 Wood Haven Bridges (backyard), Greenfield, WI 5 Wood Haven Bridges (backyard), Greenfield, WI 6 Seminary Woods, St. Francis WI (Oak) Seminary Woods, St. Francis, WI (Maple) 6 1,025 8 Lenox, Milwaukee, WI backyard (under pear tree) 9 Lenox, Milwaukee, WI backyard (under Maple Tree) 5 1,
6 ph Number of Bacterial Colonies 10 Crawford, St. Francis, WI, backyard (Under Birch Tree) Pine Soil Type Non-Pine Figure 1. Mean (+/- S.D.) of the number of bacterial colonies in pine and non-pine tree soil Pine Soil Type Non-Pine Figure 2. Mean (+/- S.D.) of the ph values in both the pine and non-pine tree soils
7 Discussion The data did support the hypothesis that soil in areas where there is coniferous tree growth will have less bacterial growth than in areas with no coniferous tree growth. The data was similar to the findings of Skyllberg et al (2001), who found that coniferous tree soil can have less bacterial growth because the pine tree needles make the ground more acidic, which is usually not an optimal ph for bacteria to grow. Bacteria will most likely grow at a neutral ph. Although the hypothesis was supported, there were limitations to this experiment. Based on our data, there was more bacterial growth in the soil surrounding deciduous trees, but the ph values for each location for both soil types was very similar. The soil ph of the coniferous trees seemed to be more basic, while the deciduous tree soil was more neutral. This could be because the soil that we sampled for this experiment was exposed to a lot of water because days prior to experimentation, it was very rainy. This could have caused leaching or other disruptions to the soil. During experimentation we checked the ph of each soil sample twice, to be sure that we had an accurate reading. Both of the soil types could have had very similar readings because the ph strips could have been old or there could have been fertilizer in one of our soil samples that we were unable to identify. In addition, we also changed our incubation from 48 to 24 hours because our bacterial growth was high after 24 hours. The increased bacterial growth after 24 hours might be due to contamination, but we performed sterile procedure while inoculating the plates. If we were to repeat this experiment in the future we would have collected soil samples are different locations. We would collect our samples at different sites far away from the city, in some local forest were we knew for sure that the soil was natural, without any fertilizers being added, which could interfere with our ph readings and or bacterial colony count. We would have also tried a different method for plating. Some of the plates were easier to count than others. If we had incubated the plates for longer than 24 hours, there might be confluent growth on our plates, making it very difficult to discern which soil type had the most growth and the least.
8 Through these improvements, we believe we would have been able to find more information about soil ph and bacterial growth. In particular, we would like to learn more about the types of bacteria that live in Wisconsin soils. This further research will help us gain insight into what are optimum conditions for different soil bacterial communities and how soil ph can fluctuate just by the vegetation it surrounds.
9 LITERATURE CITED Angers, D.A. and Caron, J. (1998). Plant induced changes in soil structure: Processes and feedbacks. Biogeochemistry, 42, Retrieved September 10, 2012, from JSTOR Database. Chamier, A. (1987). Effect of ph on Microbial Degradation of Leaf Litter in Seven Streams of the English Lake District. Oecologia, 71(4), Retrieved September 11, 2012 from JSTOR Database. Fierer, N. and Jackson, R.B. (2005). The diversity and biogeography of soil bacterial communities. Proceedings of the National Academy of Sciences of the United States of America, 103, 3, Retrieved September 9, 2012, from JSTOR Database. Skyllberg, U., Raulund-Rasmussen, K. and Borggaard, O.K. (2001). ph buffering in acidic soils developed under Picea abies and Quercus robur-effects of soil organic matter, absorbed cations and soil solution ionic strength. Biogeochemistry, 56, 1, Retrieved September 10, 2012 from JSTOR Database.
Lab Exercise 3: Media, incubation, and aseptic technique
Lab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. 2. Describe the different formats of media, plate, tube etc. 3. Explain how to sterilize it,
More informationTransferring a Broth Culture to Fresh Broth
Sterile Technique It is very important in microbiology to work with pure cultures. Unfortunately this is difficult. The world around us is covered with microorganisms. Microorganisms are even carried on
More informationBACTERIAL ENUMERATION
BACTERIAL ENUMERATION In the study of microbiology, there are numerous occasions when it is necessary to either estimate or determine the number of bacterial cells in a broth culture or liquid medium.
More informationNO-TILL AND NITROGEN FIXING INOCULANTS
NO-TILL AND NITROGEN FIXING INOCULANTS Tom Wacek R & D Director for Urbana Laboratories St. Joseph, Missouri Nitrogen fixation is the utilization of the free gaseous nitrogen in the air by soil bacteria
More informationIn order to be useful, a smear must have the following qualities:
Smear Preparation and Simple Stain Objectives: Make bacterial smear slides (usually called smears) Distinguish cells on these slides using a simple stain procedure Unstained microbial cells are nearly
More informationLab 7 Soil ph and Salinity OBJECTIVE INTRODUCTION Soil ph active
Lab 7 Soil ph and Salinity OBJECTIVE In this lab you will learn the effect of the concentration of hydrogen ions (ph) and various salts on the fertility of a soil. You will perform some tests which are
More informationThe Effects of Acid Rain on the Fast Plant Brassica Rapa Author(s) Redacted
The Effects of Acid Rain on the Fast Plant Brassica Rapa Author(s) Redacted Introduction This experiment was designed to discover whether or not acid rain stunts the growth of the fast plant brassica rappa.
More informationHow Much Does Acid Rain Hinder the Growth Height of Brassica rapa Plants Without Other Environmental Stressors?
How Much Does Acid Rain Hinder the Growth Height of Brassica rapa Plants Without Other Environmental Stressors? Author(s) Redacted Abstract: Brassica rapa plants are one of many plants in the environment
More informationDesigning An Experiment Using Baking Soda and Vinegar
Designing An Experiment Using Baking Soda and Vinegar Introduction: Kinetics is the study of chemical reaction rates. It is the study of how fast different chemicals react with one another to form new
More informationEnzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity Experiment 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
More informationLAB 4. Cultivation of Bacteria INTRODUCTION
LAB 4. Cultivation of Bacteria Protocols for use of cultivation of bacteria, use of general growth, enriched, selective and differential media, plate pouring, determination of temperature range for growth
More informationLab Exercise 2 Media and Culture
Lab Exercise 2 Media and Culture Lab Exercise #2 Bacterial Media & Culture I. OBJECTIVES: Practice microbial collection techniques Describe colony morphology and the relationship to microbial identification.
More informationEffects of Antibiotics on Bacterial Growth and Protein Synthesis: Student Laboratory Manual
Effects of Antibiotics on Bacterial Growth and Protein Synthesis: Student Laboratory Manual I. Purpose...1 II. Introduction...1 III. Inhibition of Bacterial Growth Protocol...2 IV. Inhibition of in vitro
More informationDisc Diffusion Susceptibility Methods
Disc Diffusion Susceptibility Methods Introduction When a filter paper disc impregnated with a chemical is placed on agar the chemical will diffuse from the disc into the agar. This diffusion will place
More informationTransformation of the bacterium E. coli. using a gene for Green Fluorescent Protein
Transformation of the bacterium E. coli using a gene for Green Fluorescent Protein Background In molecular biology, transformation refers to a form of genetic exchange in which the genetic material carried
More informationPLANET EARTH: Seasonal Forests
PLANET EARTH: Seasonal Forests Teacher s Guide Grade Level: 6-8 Running Time: 42 minutes Program Description Investigate temperate forests and find some of the most elusive creatures and welladapted plant
More informationLAB 5 - PLANT NUTRITION. Chemical Ionic forms Approximate dry Element symbol Atomic weight Absorbed by plants tissue concentration
LAB 5 PLANT NUTRITION I. General Introduction All living organisms require certain elements for their survival. Plants are known to require carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus
More informationTransformation Protocol
To make Glycerol Stocks of Plasmids ** To be done in the hood and use RNase/DNase free tips** 1. In a 10 ml sterile tube add 3 ml autoclaved LB broth and 1.5 ul antibiotic (@ 100 ug/ul) or 3 ul antibiotic
More informationFor all turfgrass areas. Now you can test soil reaction at different levels in the soil profile. Check Soil ph on:
Turf-Tec ph Meter Test soil ph in the mat, thatch and above and below the root zone. ph can be checked, 0" to 4" inch level right on the turfgrass area. Simply insert probe to desired depth, allow to stand
More informationScience in the Real World Microbes In Action
Science in the Real World Microbes In Action Edited by: Teresa Thiel, Ph.D. University of Missouri-St. Louis Program Director & Microbiologist Slick Oil Lab is a curriculum unit developed as part of the
More informationLab 10: Bacterial Transformation, part 2, DNA plasmid preps, Determining DNA Concentration and Purity
Lab 10: Bacterial Transformation, part 2, DNA plasmid preps, Determining DNA Concentration and Purity Today you analyze the results of your bacterial transformation from last week and determine the efficiency
More informationQuantifying Bacterial Concentration using a Calibrated Growth Curve
BTEC 4200 Lab 2. Quantifying Bacterial Concentration using a Calibrated Growth Curve Background and References Bacterial concentration can be measured by several methods, all of which you have studied
More informationBUGS" THAT PRODUCE DRUGS TO KILL "BUGS Microbes Produce Antibiotics
BUGS" THAT PRODUCE DRUGS TO KILL "BUGS Microbes Produce Antibiotics Science in the Real World Microbes In Action BUGS" THAT PRODUCE DRUGS TO KILL "BUGS is a curriculum unit developed as part of the Science
More informationEnzymes: Amylase Activity in Starch-degrading Soil Isolates
Enzymes: Amylase Activity in Starch-degrading Soil Isolates Introduction This week you will continue our theme of industrial microbiologist by characterizing the enzyme activity we selected for (starch
More informationMICROSCOPY OF LIVING MICROBES
EXPERIMENT 1 MICROSCOPY OF LIVING MICROBES Many students taking microbiology for the first time feel that they are going to have a hard time with the microscope. This lab as an experiment is intended to
More informationMicrobiology BIOL 275 DILUTIONS
DILUTIONS Occasionally a solution is too concentrated to be used as is. For example, when one is performing manual blood counts, the blood contains too many cells to be counted as such. Or when performing
More informationPlant Growth - Light and Shade
Science Unit: Lesson 5: Plants Plant Growth - Light and Shade School year: 2004/2005 Developed for: Developed by: Grade level: Duration of lesson: Notes: Queen Alexandra Elementary School, Vancouver School
More informationTeacher Demo: Photosynthesis and Respiration: Complementary Processes
SNC1D/1P Sustainable Ecosystems/ Sustainable Ecosystems and Human Activity Teacher Demo: Photosynthesis and Respiration: Complementary Processes Topics photosynthesis and respiration gas tests for oxygen
More informationTransformation Kit BACTERIAL TRANSFORMATION: GREEN FLUORESCENT PROTEIN. Partnership for Biotechnology and Genomics Education
Transformation Kit BACTERIAL TRANSFORMATION: GREEN FLUORESCENT PROTEIN Partnership for Biotechnology and Genomics Education Barbara Soots Linda Curro Education Coordinator University of California Davis
More informationNational Food Safety Standard Food microbiological examination: Aerobic plate count
National Food Safety Standard of the People s Republic of China GB4789.2-2010 National Food Safety Standard Food microbiological examination: Aerobic plate count Issued by 2010-03-26 Implemented by 2010-06-01
More informationShampoo Properties Evaluation General Science
/ 10 Shampoo Properties Evaluation General Science Name It is difficult to obtain exact information on the formulation of commercial shampoos. These facts are held by the manufacturer to protect their
More informationBacterial Transformation with Green Fluorescent Protein. Table of Contents Fall 2012
Bacterial Transformation with Green Fluorescent Protein pglo Version Table of Contents Bacterial Transformation Introduction..1 Laboratory Exercise...3 Important Laboratory Practices 3 Protocol...... 4
More informationThe Relationship between ph and Deionized Water
The Relationship between ph and Deionized Water The basics of ph The topic of ph and water has been well documented over the years; however, there is still much confusion about its significance in high
More informationEnzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity Experiment 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
More informationProperties of Acids and Bases
Lab 22 Properties of Acids and Bases TN Standard 4.2: The student will investigate the characteristics of acids and bases. Have you ever brushed your teeth and then drank a glass of orange juice? What
More informationWays of Making Terra Preta: Biochar Activation
Ways of Making Terra Preta: Biochar Activation by Hans-Peter Schmidt (Delinat-Institute) Biochar is not a fertilizer, but rather a nutrient carrier and a habitat for microorganisms. First of all it has
More informationHiPer Ion Exchange Chromatography Teaching Kit
HiPer Ion Exchange Chromatography Teaching Kit Product Code: HTC001 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 5-6 hours Storage Instructions: The kit is stable for
More informationAerobic Count. Interpretation Guide. 3M Food Safety 3M Petrifilm Aerobic Count Plate
3M Food Safety 3M Petrifilm Aerobic Count Plate Aerobic Count Interpretation Guide The 3M Petrifilm Aerobic Count (AC) Plate is a ready-made culture medium system that contains Standard Methods nutrients,
More informationENUMERATION OF MICROORGANISMS. To learn the different techniques used to count the number of microorganisms in a sample.
ENUMERATION OF MICROORGANISMS I. OBJECTIVES To learn the different techniques used to count the number of microorganisms in a sample. To be able to differentiate between different enumeration techniques
More informationUTILIZATION of PLASMA ACTIVATED WATER in Biotechnology, Pharmacology and Medicine. JSC TECHNOSYSTEM-ECO Moscow, Russia April, 2009
UTILIZATION of PLASMA ACTIVATED WATER in Biotechnology, Pharmacology and Medicine JSC TECHNOSYSTEM-ECO Moscow, Russia April, 2009 METHOD of WATER ACTIVATION with PLASMA of GAS DISCHARGE ANODE VACUUM WATER
More informationSample Liver Enzyme Lab
Sample Liver Enzyme Lab Design Aspect 1: Research Question This lab will be driven by the research question, Do changes in temperature have an effect on the activity of the enzyme catalase? Pearson Baccalaureate:
More informationIntroduction. ph = log [H + ]
Visualizing ph 2010, 1992 by David A. Katz. All rights reserved. Permission granted for classroom use. All reproductions must include original copyright. David A. Katz Chemist, Educator, Science Communicator,
More informationBluelab Soil ph Meter Instruction Manual
Instruction Manual www.getbluelab.com Table of Contents 1.0 Information about measuring the ph of soils/media. 2 2.0 Introduction to Bluelab Soil ph Meter 4 3.0 Preparing the Bluelab Soil ph Meter for
More informationMeasuring ph in Water or CaCl2 Using a ph Meter. Compiled by Darren Murray, June 30, 2011 TABLE OF CONTENTS OVERVIEW 3
Measuring ph in Water or CaCl2 Using a ph Meter Compiled by Darren Murray, June 30, 2011 TABLE OF CONTENTS OVERVIEW 3 PREPARING THE ph METER FOR USE (Calibration and Buffer Solutions) 4 When is Calibration
More informationEffect of moisture on lentil seed germination and growth
Effect of moisture on lentil seed germination and growth By Francisco Javier Hidalgo Zamora, 7º A (This experiment was carried out in collaboration with Robert Henshaw Amado) Objective/Hypothesis The objective
More informationLAB TOPIC 4: ENZYMES. Enzyme catalyzed reactions can be expressed in the following way:
LAB TOPIC 4: ENZYMES Objectives Define enzyme and describe the activity of enzymes in cells. Discuss the effects of varying enzyme concentrations on the rate of enzyme activity. Discuss the effects of
More informationThe purpose of this lab is to investigate the impact of temperature, substrate concentration,
Lee 1 Jessica Lee AP Biology Mrs. Kingston 23 October 2013 Abstract: The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence
More informationBiological Sciences Initiative
Biological Sciences Initiative HHMI Student Activities Measuring Antibiotic Resistance Introduction: You might be aware that antibiotics were once thought of as a magic bullet; a nearly perfect drug for
More informationPlants, like all other living organisms have basic needs: a source of nutrition (food),
LEARNING FROM LEAVES: A LOOK AT LEAF SIZE Grades 3 6 I. Introduction Plants, like all other living organisms have basic needs: a source of nutrition (food), water, space in which to live, air, and optimal
More informationIsolation of Starch degrading bacteria Enzymes in Action
Isolation of Starch degrading bacteria Enzymes in Action Introduction In this laboratory exercise, you will be playing the role of biotechnologists in search of a new amylase. Since most industrially used
More informationResult: COMPLETE Report Date: 08-OCT-2012
Send To: 1X220 Ms. Laura Marshall AcornVac, Inc. 13818 Oaks Avenue Chino CA 91710 United States Facility: 1X221 AcornVac, Inc. 13818 Oaks Avenue Chino CA 91710 United States Result: COMPLETE Report Date:
More informationPractice Questions 1: Scientific Method
Practice Questions 1: Scientific Method 1. A student divided some insect larvae into four equal groups, each having the same amount of food. Each group was kept at a different temperature, and the average
More informationLaboratory 5: Properties of Enzymes
Laboratory 5: Properties of Enzymes Technical Objectives 1. Accurately measure and transfer solutions with pipettes 2. Use a Spectrophotometer to study enzyme action. 3. Properly graph a set of data. Knowledge
More informationMicrobiological Testing of the Sawyer Mini Filter. 16 December 2013. Summary
Microbiological Testing of the Sawyer Mini Filter 16 December 2013 Summary The Sawyer Mini Filter was tested for its ability to remove three microorganisms Raoultella terrigena, Bacillus subtilis, and
More informationPotato Microbiology. Sarah Follenweider, The English High School 2009 Summer Research Internship Program
Potato Microbiology Sarah Follenweider, The English High School 2009 Summer Research Internship Program Introduction: A number of microorganisms thrive on the nutrients that can be found in a potato. My
More informationLAB 11 PLASMID DNA MINIPREP
LAB 11 PLASMID DNA MINIPREP STUDENT GUIDE GOAL The objective of this lab is to perform extraction of plasmid DNA and analyze the results. OBJECTIVES After completion, the student should be able to: 1.
More informationThis laboratory explores the affects ph has on a reaction rate. The reaction
Joy Paul Enzyme Catalyst lab Abstract: This laboratory explores the affects ph has on a reaction rate. The reaction studied was the breakdown of hydrogen peroxide catalyzed by the enzyme peroxidase. Three
More informationUltraClean Soil DNA Isolation Kit
PAGE 1 UltraClean Soil DNA Isolation Kit Catalog # 12800-50 50 preps New improved PCR inhibitor removal solution (IRS) included Instruction Manual (New Alternative Protocol maximizes yields) Introduction
More informationTransformAid Bacterial Transformation Kit
Home Contacts Order Catalog Support Search Alphabetical Index Numerical Index Restriction Endonucleases Modifying Enzymes PCR Kits Markers Nucleic Acids Nucleotides & Oligonucleotides Media Transfection
More informationph and CONDUCTIVITY ph and conductivity in fountain solution are necessary for plate humidification.
ph and CONDUCTIVITY ph and conductivity in fountain solution are necessary for plate humidification. CONTROL OF VARIATIONS OF FOUNTAIN SOLUTION CONCENTRATE FOR A BETTER PRINTING QUALITY Two major control
More informationJennifer Cosgrove & Julie Ertmann Developer of Curriculum Unit
Science in the Real World Microbes In Action Spice Up Your Life! is a curriculum unit developed as part of the Science In The Real World: Microbes In Action Program. The curriculum units were developed
More informationph Value of Common Household Items and the Environmental Effects ph on Water; http://www.epa.gov/acidrain/education/site_students/phscale.
ACID RAIN What is acid rain? Acid rain is a broad term that is often used to describe several forms of acid deposition. Wet deposition is when rain, snow, fog, or mist contains high amounts of sulfuric
More informationThe Determination of an Equilibrium Constant
The Determination of an Equilibrium Constant Computer 10 Chemical reactions occur to reach a state of equilibrium. The equilibrium state can be characterized by quantitatively defining its equilibrium
More informationTEACHER ACTIVITY GUIDE
Page 1/5 TEACHER ACTIVITY GUIDE EFFECT OF HEAT & ph ON COLOR & TEXTURE OF GREEN VEGETABLES Taken from IFT Experiments in Food Science Series Color plays a key role in establishing consumer acceptability
More informationCatalytic Activity of Enzymes
Catalytic Activity of Enzymes Introduction Enzymes are biological molecules that catalyze (speed up) chemical reactions. You could call enzymes the Builders and Do-ers in the cell; without them, life could
More informationSUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL (Student Instructions) Determination of the Formula of a Hydrate A Greener Approach Objectives To experimentally determine the formula of a hydrate salt. To learn to think in terms
More informationPlants, like all other living organisms have basic needs: a source of nutrition (food),
WHAT PLANTS NEED IN ORDER TO SURVIVE AND GROW: WATER Grades 3 6 I. Introduction Plants, like all other living organisms have basic needs: a source of nutrition (food), water, space in which to live, air,
More informationFertility Guidelines for Hops in the Northeast Dr. Heather Darby, University of Vermont Extension Agronomist
Fertility Guidelines for Hops in the Northeast Dr. Heather Darby, University of Vermont Extension Agronomist The increasing acreage of hops in the Northeast has prompted the need for fertility guidelines
More informationEnzyme Action: Testing Catalase Activity
Enzyme Action: Testing Catalase Activity DataQuest 12 Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the chemical activities
More informationMud Power Research Plan
Mud Power Research Plan By Jonathan Rieco 8th grade Flat Rock Middle School Abstract Man kind is always trying to look for a more effective way of producing power because we can never seem to find a way
More informationDetermining Equivalent Weight by Copper Electrolysis
Purpose The purpose of this experiment is to determine the equivalent mass of copper based on change in the mass of a copper electrode and the volume of hydrogen gas generated during an electrolysis reaction.
More informationHuman Physiology Lab (Biol 236L) Digestive Physiology: Amylase hydrolysis of starch
Human Physiology Lab (Biol 236L) Digestive Physiology: Amylase hydrolysis of starch Introduction Enzymes are proteins composed of amino acid building blocks. Enzymes catalyze or increase the rate of metabolic
More informationcatalase 2H 2 O 2 (l) ----> 2H 2 O (l) + O 2 (g)
ENZYME POST LAB QUIZ STUDY GUIDE Below are the answers to the post-lab (Data Analysis) questions. Make sure you UNDERSTAND all of these questions. The post-lab questions will, of course, be different,
More information* Readily available from supermarkets and health food stores. 80-145 minutes (total over 3 to 7 days) SOSE: Investigate Australia s salinity crisis
Lesson Plan 12 Salt and germination Brief description This fascinating, cheap and very reliable experiment clearly demonstrates the damaging effects of salinity (salt) on seed germination. Mung beans are
More informationHazardous Waste Procedures
Hazardous Waste Procedures Hazardous waste is defined as a waste, or combination of wastes, which because of its quantity, concentration, or physical or chemical characteristics may pose a substantial
More informationThe Empirical Formula of a Compound
The Empirical Formula of a Compound Lab #5 Introduction A look at the mass relationships in chemistry reveals little order or sense. The ratio of the masses of the elements in a compound, while constant,
More informationIn this lesson, students will identify a local plant community and make a variety of
MAKING COMMUNITY MEASUREMENTS: ABIOTIC FACTORS Grades 3 6 I. Introduction In this lesson, students will identify a local plant community and make a variety of measurements, preferably during two different
More informationENZYME ACTION: TESTING CATALASE ACTIVITY
ENZYME ACTION: TESTING CATALASE ACTIVITY LAB ENZ 1.CALC From Biology with Calculators, Vernier Software & Technology, 2000 INTRODUCTION Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically.
More informationAcknowledgements. Developing collaborative lab experiments across disciplines through the identification of bacteria
Acknowledgements Developing collaborative lab experiments across disciplines through the identification of bacteria Joanna Huxster, Ph.D. Sarah Moss, MS 15 Emily Bilyk, BS 16 Brian M. Forster, Ph.D. Lab
More informationProcess 3.5. A Pour it down the sink. B Pour it back into its original container. C Dispose of it as directed by his teacher.
Process 3.5 Biology EOI sample test questions Objective numbers correspond to the State Priority Academic Student Skills (PASS) standards and objectives. This number is also referenced with the local objective
More informationWhat is Acid Rain and What Causes It?
What is Acid Rain and What Causes It? Acid rain is a broad term used to describe several ways that acids fall out of the atmosphere. A more precise term is acid deposition, which has two parts: wet and
More informationFat Content in Ground Meat: A statistical analysis
Volume 25: Mini Workshops 385 Fat Content in Ground Meat: A statistical analysis Mary Culp Canisius College Biology Department 2001 Main Street Buffalo, NY 14208-1098 culpm@canisius.edu Mary Culp has been
More informationCalcium Analysis by EDTA Titration
Calcium Analysis by EDTA Titration ne of the factors that establish the quality of a water supply is its degree of hardness. The hardness of water is defined in terms of its content of calcium and magnesium
More informationEnzyme Action: Testing Catalase Activity 50 Points
Names: LabQuest Enzyme Action: Testing Catalase Activity 50 Points 6A Many organisms can decompose hydrogen peroxide (H 2 O 2 ) enzymatically. Enzymes are globular proteins, responsible for most of the
More informationThe Basic Humic Acid Products
The Basic Humic Acid Products Over at least twelve years of actual field experience with an ample variety of humic acid formulations (some of them developed many years before) have given us a singular
More informationBiogas. Biology Teams of 2 or 3. Grade 10-12 LEARNING OUTCOMES DESCRIPTION MATERIALS READINESS ACTIVITIES. Science
Science Grade 10-12 Lab Teams of 2 or 3 DESCRIPTION Biogas is a fuel which is produced from the breakdown of organic matter. Students will have an opportunity to create their own biogas generators. At
More informationNNIN Nanotechnology Education
NNIN Nanotechnology Education How Quickly Do Bacteria Grow? Teacher s Guide Purpose: Students will relate real-world applications to mathematical concepts by monitoring bacterial growth over one week and
More informationKey Idea 2: Ecosystems
Key Idea 2: Ecosystems Ecosystems An ecosystem is a living community of plants and animals sharing an environment with non-living elements such as climate and soil. An example of a small scale ecosystem
More informationEFFECT OF A MICROBIAL INOCULANT ON GROWTH AND CHLOROPHYLL LEVEL OF LETTUCE AND RADISH SEEDLINGS: A PRELIMINARY STUDY
EFFECT OF A MICROBIAL INOCULANT ON GROWTH AND CHLOROPHYLL LEVEL OF LETTUCE AND RADISH SEEDLINGS: A PRELIMINARY STUDY Susan Carrodus Lincoln University 2002 INTRODUCTION Microorganisms are often used in
More informationWhich of the following can be determined based on this model? The atmosphere is the only reservoir on Earth that can store carbon in any form. A.
Earth s Cycles 1. Models are often used to explain scientific knowledge or experimental results. A model of the carbon cycle is shown below. Which of the following can be determined based on this model?
More informationWhat Is Humic Acid? Where Does It Come From?
What Is Humic Acid? Humic and Fulvic acids are the final break-down constituents of the natural decay of plant and animal materials. These organic acids are found in pre-historic deposits. Humic matter
More informationDETECTION OF BACTERIAL MOTILITY. To demonstrate bacterial motility by microscopic and macroscopic techniques.
DETECTION OF BACTERIAL MOTILITY I. OBJECTIVES To demonstrate bacterial motility by microscopic and macroscopic techniques. To observe flagella in prepared slides stained by specific flagellar stains. II.
More informationNitrogen Fixing Bacteria in Agriculture Now a Real Option Guy Webb B.Sc. REM Agricultural Consultant
Nitrogen Fixing Bacteria in Agriculture Now a Real Option Guy Webb B.Sc. REM Agricultural Consultant The Pursuit of Protein and Profit All agricultural enterprises, in essence, are based on the pursuit
More informationTHE ETCHING OF MARBLE BY ROOTS IN THE PRESENCE AND ABSENCE OF BACTERIA.*
THE ETCHING OF MARBLE BY ROOTS IN THE PRESENCE AND ABSENCE OF BACTERIA.* BY E. B. FRED AND A. R. C. HAAS. (From the Department of Agricultural Bacteriology, University of Wisconsin, Madison.) (Received
More informationChapter 3: Water and Life
Name Period Chapter 3: Water and Life Concept 3.1 Polar covalent bonds in water result in hydrogen bonding 1. Study the water molecules at the right. On the central molecule, label oxygen (O) and hydrogen
More informationRecovery of Elemental Copper from Copper (II) Nitrate
Recovery of Elemental Copper from Copper (II) Nitrate Objectives: Challenge: Students should be able to - recognize evidence(s) of a chemical change - convert word equations into formula equations - perform
More informationDetermination of calcium by Standardized EDTA Solution
Determination of calcium by Standardized EDTA Solution Introduction The classic method of determining calcium and other suitable cations is titration with a standardized solution of ethylenediaminetetraacetic
More informationAgrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola and Colletotrichum sublineolum Flowers and Vaillancourt, 2005. Current Genetics 48: 380-388 NOTE added by L. Vaillancourt:
More informationDose/Response Experiments on Lettuce Seeds
Dose/Response Experiments on Lettuce Seeds Name: Date: Lab# Bioassays: Background Information on Dose/Response Experiments A bioassay involves use of a biological organism to test for chemical toxicity.
More informationBiology 3A Laboratory: Enzyme Function
Biology 3A Laboratory: Enzyme Function Objectives To be able to list the general characteristics of enzymes. To study the effects of enzymes on the rate of chemical reactions. To demonstrate the effect
More information