BLOOD PHYSIOLOGY Lecture 3 BLOOD TYPES AND BLOOD TRANSFUSION. 2nd year Dentistry
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1 BLOOD PHYSIOLOGY Lecture 3 BLOOD TYPES AND BLOOD TRANSFUSION 2nd year Dentistry
2 Objectives 1. Describe the basis of blood typing 2. Know the ABO and Rh systems for blood typing and their clinical significance. 3. Describe incompatibilities in ABO and Rh systems. 4. Know the different types of blood transfusions and their complications (transfusion reactions). 5. Describe the basis of blood typing and crossmatching tests for a safe blood transfusion. 2nd year Dentistry
3 Basis of Blood grouping or Typing (Multiplicity of Antigens in the blood cells) At least 30 commonly occurring antigens have been found on the cell membrane of RBCs. These can cause Ag Ab (antibody) reaction if mixed with plasma that contain Ab against these Ag. According to presence or absence of these antigens blood is classified into several groups or types. Two groups of Ag can cause transfusion reactions more than others: ABO and Rh systems of Ag. 2nd year Dentistry
4 ABO System for Blood Typing A and B Antigens Agglutinogens 2nd year Dentistry
5 ABO blood types Relative frequency of different blood types: O 47% A 41% B 9% AB 3% 2nd year Dentistry
6 Agglutinins (antibodies) They are naturally occurring antibodies Blood type Agglutinogen on RBC Agglutinin in plasma A A Anti B B B Anti A AB A and B None O None Anti A and Anti B 2nd year Dentistry
7 2nd year Dentistry
8 Rh System for Blood Typing If type D antigen is present on RBC Rh +ve Differences between ABO and Rh Ab? Anti Rh antibodies are not naturally occurring Ab. Previous exposure to Rh antigen is required. Rh +ve blood transfusion. Rh ve women pregnant with Rh +ve baby. Anti Rh Ab can cross the placenta. 2nd year Dentistry
9 Haemolytic Disease of the Newborn (HDN) Rh incompatibility Erythroblastosis Fetalis (HDN). Rh ve lady marrying Rh+ve man. If baby is Rh+ve, fetal RBC leaks to maternal circulation during placental separation (delivery or abortion). Mother starts to make anti Rh Ab. Next pregnancy with Rh+ve baby anti Rh Ab pass to baby and cause agglutination and hemolysis of his RBC. 9
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11 Prevention Anti D antibodies (RhoGam) injection given to Rh ve mothers after delivery of Rh +ve baby. 2nd year Dentistry
12 1 minute Quiz How does a RhoGam injection (anti D antibodies) prevent HDN in a future pregnancy with an Rh +ve baby? 2nd year Dentistry
13 2nd year Dentistry
14 BLOOD TRANSFUSIONS 2nd year Dentistry
15 Blood Transfusion Indications 1. Haemorrhage 2. Loss of one element of blood as in anemia, leucopenia and purpura Types: Heterologus from other person of the same species Autologous from the same person 2nd year Dentistry
16 Transfusion Reactions resulting from mismatched blood types Agglutination and delayed hemolysis of donor s RBC (or immediate intravascular hemolysis) Jaundice Renal failure: Renal tubular blockage by hemoglobin 2nd year Dentistry
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18 Pre transfusion Tests For a safe blood transfusion, the following tests are done: Blood typing Cross matching 2nd year Dentistry
19 Blood Typing 2nd year Dentistry
20 Blood typing, showing agglutination of cells of the different blood types with anti A or anti B agglutinins in the sera Red blood cell types Anti A serum O A + B + Anti B serum AB + + 2nd year Dentistry
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25 X matching Once patient s blood type is known, donor blood of the same ABO and Rh type is selected. Possible donor RBC s are mixed with the recipient s serum. If no agglutination, no Ab in recipient blood will attack donor s RBCs. 2nd year Dentistry
26 Finally Write down the muddiest point. Summarize the lecture in your own words. 2nd year Dentistry
27 Summary What are the basis of blood type classification into O A B and Rh blood types? What are the differences in the agglutinins (antibodies) against the O A B and Rh system agglutinogens (antigens)? What is the cause of HDN? What are the consequences of an incompatible blood transfusions (transfusion reactions)? What laboratory tests are done to insure a safe blood transfusion? 2nd year Dentistry
28 Thank You for Not Listening 2nd year Dentistry
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