CERTIFICATE OF ACCREDITATION

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1 CERTIFICATE OF ACCREDITATION In terms of section 22(2) (b) of the Accreditation for Conformity Assessment, Calibration and Good Laboratory Practice Act, 2006 (Act 19 of 2006), read with sections 23(1), (2) and (3) of the said Act, I hereby certify that:- BOTSWANA NATIONAL VETERINARY LABORATORY BOTSWANA Facility Accreditation Number: V0012 is a South African National Accreditation System accredited Testing laboratory provided that all SANAS conditions and requirements are complied with This certificate is valid as per the scope as stated in the accompanying schedule of accreditation Annexure A, bearing the above accreditation number for MICROBIOLOGY, SEROLOGY, VIROLOGY, CHEMISTRY HISTOPATHOLOGY, BACTERIOLOGY AND MOLECULAR BIOLOGY The facility is accredited in accordance with the recognised International Standard ISO/IEC 17025:2005 The accreditation demonstrates technical competency for a defined scope and the operation of a laboratory quality management system While this certificate remains valid, the Accredited Facility named above is authorised to use the relevant SANAS accreditation symbol to issue facility reports and/or certificates Dr EJ Steyn Acting Chief Executive Officer Effective Date: 10 November 2012 Certificate Expires: 09 November 2017

2 ANNEXURE A SCHEDULE OF ACCREDITATION Facility Number: V0012 Permanent Address of Laboratory: Technical Signatories: Mrs L Khohliwe Micro All (Except: 283 DH and Botswana National Veterinary Laboratory 0550 BACT) Content Farm Mr E Dikgang Micro (Water, Food, Feeding Stuff) Off Gaborone Francistown Road (A1) Mr I Saudu Micro (Food, Water and Feed Stuff) Mrs D Tladi Micro (Food, Water and Feed Stuff) Sebele Ms H Mahloane Micro 0035, 0283, 0315 and 0424 Mr T Tswiio Micro (0035, 0283, 0315 and 0424 Mrs B Gaobewe Micro (0035, 0283, 0315 and 0424 Mr M Moeng Micro 0035, 0283, 0315 and 0424 Mr P Tikologo Micro 0035, 0283, 0315 and 0424 Botswana Vaccine Institute (BVI) Plot 6385/90 Mr T Kootshegetse Micro 0035, 0283, 0315 and 0424 Lejara Road Mr M Palai Serology (0530 and 0781 SERO) Broadhurst Industrial Mr S Sediadie Serology (0510, 0512 and 0165 Gaborone 0530 SERO) Mr K Monyame Serology (0210, 0282 (HI), 0097 VIRO & ELISA) Dr K Moagabo Serology (0210, 0282 (HI) & ELISA) Dr M Kawonga Serology (0395 PARA) Ms L Dinakanyane Serology (0395 PARA) Ms K Mangate Serology (0395 PARA) Mr K Dipuo Serology (0510, 0512, and 0165 SERO) Mr E Dihoro Serology (0530 SERO) Ms K Magama Histopathology (0003 BIO) Mr KS Thitoyamore Histopathology (0003 BIO) Ms G Modise- Histopathology (0003 BIO) Mr P Kgothi Chemistry (0090 RES) Mr S.O Mookantsa Chemistry (0090, 0065, 0030 and 0029 RES) Ms O Kemokgatla Chemistry (0785, 0753 and 0019 RES) Mrs K Gaolape Chemistry (0066 and 0020 RES) Mr R Tshepho Chemistry (0060, 0066 and 0017 RES) Mr P Kolanyane - Chemistry (0026 RES) Ms B Modise Molecular Biology (0640 MB) Mrs K Masoba - Molecular Biology (0640 MB) Ms T Kgotlele - Molecular Biology (0640 MB) Ms OS Sekaba Bacteriology (0550 BACT) Ms TM Bagali Bacteriology (0550 BACT) Mr EM Fana Virology (0097, 0121 and 0100 VIR) Dr S Letsholo Virology (0210 and 0282 VIR) Original Date of Accreditation: 10 November 2007 Page 1 of 5

3 Field Manager ANNEXURE A Management Signatories: Dr C Marobela-Raborokgwe Mrs TC Losho Dr. M Kawonga Dr K T Moagabo Mr AG Ramocha Postal Address: Nominated Representative: Mr R Mackenzie Private Bag 0035 Gaborone Botswana Tel: (000267) Issue No.: 10 Fax: (000267) Date of Issue: 28 January [email protected] Expiry Date: 09 November 2017 Sample Type / Materials / Products Tested Type of Tests / Properties Measured Standard Specifications, Equipment / Technique Used MICROBIOLOGY Food and Feeding Stuffs Total viable bacterial count (Standard Plate Count) Doc: BNVL/MS. WI 0006 FHS Enumeration of Staphylococcus aureus Doc: BNVL/MS. WI 0005 FHS Enumeration of total coliforms and Escherichia coli Doc: BNVL/MS. WI 0008 FHS Detection of Escherichia coli 0157 Doc: BNVL/MS. WI 0015 FHS Detection of Salmonella spp Doc: BNVL/MS. WI 0016 FHS MICROBIOLOGY Water Detection and enumeration of coliform / Escherichia coli by Doc: BNVL/MS. WI 0219 FHS membrane filtration method Detection and enumeration of intestinal enterococcus by filtration method Doc: BNVL/MS.WI 0218 FHS Food and feeding stuffs Detection and enumeration of Lactic acid bacteria By pour Doc: BNVL/MS. WI 0472 FHS plate method Detection and enumeration of Enterobacteriaceae By colony count technique Doc: BNVL/MS. WI 0450 FHS Detection and enumeration of intestinal enterococcus (faecal streptococcus) colony count technique Doc: BNVL/MS. WI 0471 FHS Detection and enumeration of sulphite reducing Clostridia (SRC) and Clostridium perfringens colony count technique Doc: BNVL/MS. WI 0292 FHS Original Date of Accreditation: 10 November 2007 Page 2 of 4

4 Field Manager ANNEXURE A Materials / Products Tested Type of Tests / Properties Measured, Range of Measurement Facility No.: V0012 Date of Issue: 11 November 2015 Expiry Date: 09 November 2017 Standard Specifications, Equipment / Technique Used Milk and milk products Enumeration of total viable microorganisms by pour plate method Doc: : BNVL/MS. WI 0035 DH Enumeration of Yeast by colony count technique Enumeration of Presumptive E coli by MPN technique Doc: BNVL/MS. WI 0424 DH Doc: BNVL/MS. WI 0315 DH Enumeration of total coliform organisms Doc: BNVL/MS. WI 0283 DH BACTERIOLOGY Tissue and Faeces Cultural isolation and identification of Doc: BNVL/MS. WI 0550 BACT Salmonella Spp. HISTOPATHOLOGY Livestock Feed Qualitative detection of processed animal Doc: BNVL/MS. WI 0003 BIO Protein by Microscopy Method. SEROLOGY (BNVL) Bovine serum Detection of contagious Bovine pleuro- Doc: BNVL/MS.WI 0165 SERO pneumonia (CBPP) antibodies serum by in Bovine complement fixation test (CFT) Equine serum Compliment Fixation Test - Dourine Doc: BNVL/MS. WI 0395 PARA Bovine serum Compliment Fixation Test - Brucellosis Doc: BNVL/MS. WI 0512 SERO Rose Bengal Plate Test - Brucellosis Doc: BNVL/MS. WI 0510 SERO Bovine Serum Liquid Phase Blocking ELISA (LPBE) Foot Doc: BNVL/MS. WI 0530 SERO and Mouth Disease (FMD) Castle, Sheep, goat and pigs sera Detection of antibodies against Non- Doc: BNVL/MS. WI 0781 SERO structural protein of Food and Mouth (NSP ELISA KIT) disease virus (NVDv) using Prio Check kits Bird and Ostrich serum Screening and titration of antibodies to Doc: BNVL/MS. WI 0210 VIR Newcastle disease virus (NVD) by HI test Screening and titration of antibodies to Avian Doc: BNVL/MS. WI 0282 VIR Original Date of Accreditation: 10 November 2007 Page 3 of 4 Field Manager

5 ANNEXURE A Materials / Products Tested Type of Tests / Properties Measured, Range of Measurement Facility No.: V0012 Date of Issue: 11 November 2015 Expiry Date: 09 November 2017 Standard Specifications, Equipment / Technique Used SEROLOGY/VIROLOGY (BVI) Lamb kidney primary cells FMD Virus isolation and identification Doc: BNVL/MS. WI 0097 VIR (serotyping) by antigen ELISA FMD Virus isolation and identification by nucleotide Doc: BNVL/MS. WI 0121 VIR sequencing FMD genome detection by RT-PCR and nucleotide Doc: BNVL/MS. WI 0100 VIR sequencing CHEMISTRY Liver Multi-residue analysis of Avermectins HPLC - FLD Doc: BNVL/MS. WI 0060 RES Liver, water Analysis of Cadmium FAAS Doc: BNVL/MS. WI 0065 RES Liver Analysis of Mercury HGCVAAS Doc: BNVL/MS. WI 0066 RES Muscle/milk Analysis of Chloramphenicol 4000 Q trap Doc: BNVL/MS. WI 0785 RES LCMS/MS Liver, water Analysis of Lead FAAS Doc: BNVL/MS. WI 0090 RES Water Analysis of Arsenic GF AAS Doc: BNVL/MS. WI 0753 RES Kidney Analysis of Sulphonamides 4000 Q trap LCMS/MS Doc: BNVL/MS.WI 0026 RES Kidney Analysis of Penicillins 4000 Q trap LCMS/MS Doc: BNVL/MS.WI 0017 RES Kidney Analysis of Quinolones 4000 Q trap LCMS/MS Doc: BNVL/MS. WI 0020 RES Kidney Analysis of Tetracyclines 4000 Q trap LCMS/MS Doc: BNVL/MS. WI 0030 RES Kidney Analysis of Lincomycin 4000 Q trap LCMS/MS Doc: BNVL/MS. WI 0019 RES Kidney Multi residue analysis of Sedative - LCMS/MS Doc: BNVL/MS. WI 0029 RES (acepromazibe, propionyl promazine, azaperol, carazolol, chlorpromazine and xylazine) MOLECULAR BIOLOGY Broth culture Detection and identification of Mycoplasma mycoides sub-species mycoides and Mycoplasma mycoides sub-species mycoides LC/Capri (PCR- RFLP) Doc: BNVL/MS. WI 0640 MB Original Date of Accreditation: 10 November 2007 Page 4 of 4 ISSUED BY THE SOUTH AFRICAN NATIONAL ACCREDITATION SYSTEM

6 Field Manager

CERTIFICATE OF ACCREDITATION

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