2008 BD Diagnostics - Diagnostic Systems

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1 2008 BD Diagnostics - Diagnostic Systems Catalog for Clinical and Industrial Microbiology Eastern Europe Middle East Africa

2 Catalog BD Diagnostics - Diagnostic Systems Eastern Europe Middle East Africa Edward J. Ludwig, Chairman, President and Chief Executive Officer Yes, we can... improve the lives of people the world over. On a daily basis, 27,000 BD associates around the globe are helping battle some of the most entrenched diseases and challenging healthcare problems. At BD, we believe the people of one company can make a difference. We are confident that our unwavering commitment - together with the innovative solutions, knowledge and expertise that are the foundation of our Company - will enable us to prevail as we strive for a healthier world. BD Diagnostics - Diagnostic Systems continues to be a leader in microbiology and infectious disease diagnostics. Its focus on growth media - for both the clinical and industrial market segments - is the foundation of strong customer relationships and an entry point for instrument platforms. For example, BD BACTEC TM Blood Culturing Systems are a critical tool for microbiologists seeking clinically relevant answers for patients with life-threatening infections. The 2006 acquisition of GeneOhm Sciences provides BD with additional molecular testing capability for the detection of bacterial organisms, especially those causing HAIs. This newly acquired capability, coupled with the BD ProbeTec TM ET System / BD Viper TM System, gives BD a growing position in molecular diagnostics. Looking forward, Diagnostic Systems will leverage its unique instrument product portfolio and engineering capabilities to provide a range of systems to rapidly diagnose infectious disease. Our European Mission Our guiding mission is to continuously support and lead success in our customers daily practices. Our key goal is to provide solutions which help our clinical and industrial clients to improve their work and information processes. We are committed to enhancing and facilitating the positive impact of our customers in the health value chain, thereby helping all people live healthy lives.

3 For over 100 years, BD has helped create stronger communities all over the world. BD continues to establish partnerships with U.S. and international nonprofit organizations to create stronger and healthier communities worldwide, fulfilling our corporate purpose of Helping all people live healthy lives. 0ARTNERS IN YEARS OF PROGRESS The March of Dimes and BD are celebrating the 50th anniversary of the Salk polio vaccine field trials. %LIMINATING MEASLES TOGETHER The American Red Cross and BD are working together in Africa to vaccinate children and educate providers about measles. 0ARTNERS PREVENTING TETANUS UNICEF and BD are working together to deliver the tetanus vaccine in developing nations. 0ARTNERS SEEKING!)$3 VACCINES IAVI and BD are working together to accelerate the development of AIDS vaccines. 0ARTNERS IN BUSINESS COMBATING ()6!)$3 GBC and BD are working together to unite the business sector in the fight against HIV/AIDS. 0ARTNERS FOR CAREGIVERS The International Healthcare Worker Safety Center is working with BD to protect healthcare workers globally. 0ARTNERS IN THE BATTLE AGAINST ()6!)$3 Save the Children, IAVI, the Clinton Foundation, and the GBC are working with BD in the fight against HIV/AIDS. 0ARTNERING AGAINST 4" FIND and BD are working together to fight TB in the developing world.

4 BD BACTEC MGIT 960 Mycobacterial Detection System The new Gold Standard in Mycobacteria Testing The BD BACTEC MGIT 960 System is the latest generation in a proven line of instruments for mycobacteria testing. It builds on the legacy of the BD BACTEC 460TB System, the world s first instrumented system for mycobacteria testing, and still a benchmark of quality and reliability. Over the years, BD BACTEC MGIT 960 has taken over the role of being the gold standard in instrumented mycobacteria testing, with the added advantage of being fully automated and nonradiometric. BD BACTEC MGIT 960 Instrument Installations: As of October 2007, 824 BD BACTEC MGIT 960 were placed in Western/Eastern Europe, Middle East, and Africa. BD is particularly proud that the following National TB Reference Laboratories are relying on BD BACTEC MGIT 960 in the fight against TB: Western Europe: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Italy, Netherlands, Norway, Portugal, Spain, Switzerland, United Kingdom Eastern Europe: Albania, Belarus, Bosnia Herzegovina, Bulgaria, Croatia, Estonia, Hungary, Latvia, Lithuania, Moldova, Poland, Russia, Serbia, Slovenia Middle East: Turkey, Saudi Arabia, Kuwait, Bahrain, Lybia, Oman, U.A.E. Africa: Angola, Botswana, Ethiopia, Ghana, Kenya, Lesotho, Namibia, Nigeria, South Africa, Swaziland, Uganda, Zambia

5 BD BACTEC MGIT 960 DST Mycobacterial Detection System Leading the fight against MDR and XDR TB WHO Liquid Culture Announcement, October 2007 WHO recommends the use of liquid cultures and rapid species identification to address the needs for culture and drug susceptibility testing (DST), integrated in a country specific comprehensive plan for laboratory capacity strengthening. The WHO liquid culture recommendation is the result of the FIND/BD Demonstration Projects. BD is proud to be a preferred partner of FIND in the fight against TB. BD EpiCenter TB-eXiST BD has developed a new set-up in its EpiCenter Data Management system. This set-up, referred to as TBeXiST (extended individual susceptibility testing), will significantly facilitate the testing and reporting of existing and potentially new 2nd-line drug results with the BD BACTEC MGIT 960. By responding and reacting rapidly to an emerging situation, BD hopes to provide a significant contribution to the fight against MDR- and XDR-TB.

6 BD GeneOhm Real-time PCR Assays... because time makes a difference! Rapid turnaround time Results within 2 hours versus 2 days Culture Testing Typical turn around time for testing can take more than two days Real-time PCR Real-time PCR direct from specimen with results in less than two hours GeneOhm s commitment is to transform patient care by delivering rapid nucleic acid-based diagnostic solutions to identify, prevent and control healthcare-associated infections. BD GeneOhm MRSA First Real-Time PCR test for MRSA screening Aids in the identification, prevention and control of MRSA Facilitates earlier intervention to prevent transmission and infection Can help control healthcare costs BD GeneOhm StaphSR First Real-Time PCR test for the simultaneous detection and differentiation of MRSA and MSSA Used directly on positive blood cultures, nasal and wound swabs (pre-surgical screening) Promotes faster, better antibiotic selection to prevent emergence of vancomycin resistance BD GeneOhm GBS First Real-Time PCR test for the rapid and reliable detection of Group B streptococcus (GBS) DNA in vaginal/rectal specimens from prepartum or intrapartum women Especially beneficial for women in preterm labor for whom culture testing is not useful because of the time it takes to receive test results Available soon: BD GeneOhm VRE and BD GeneOhm Clostridium difficile!

7 BD BACTEC Blood Culturing Systems Experience the difference Look at the major differences between BD BACTEC and other Blood Culturing Systems! n Proven fastest time to detection* n Proven best neutralization of drugs present in the patient s blood** n Special medium for fungi and yeasts with proven faster time to detection and higher recovery rate even in mixed infections* n No interference when reading Gram stain n Compatible safety devices for blood collection n Safe devices for subculturing n Most reliable blood culture instruments that rarely break down*** Choose BD BACTEC Blood Culturing Systems for: n Improved patient outcome n Improved lab efficiency n Improved safety *Carroll et al, JCM 45: , March 2007 * Vigano et al. Dian. Micro Inf. Diseases 44, 2002 ** Flyhart et al, JCM Mar *** C. Van der Linden et al, 17th ECCMID 2007, Poster R2223

8 BD EpiCenter Network Take the Right Decision A unique data management solution for microbiology laboratories to communicate and track all the information needed by every professional involved in the diagnosis and treatment of infectious diseases Real-time data access and information delivery Fully integrated, easy to use and scaleable information management Allows better monitoring of Healthcare Associated Infections (HAIs) and antimicrobial resistance for improved patient treatment Make the right decision for delivering the right results * under development

9 Table of Contents Collection and Transport Systems BD Specimen Collection & Transport Systems 1-2 Other BD Collection & Transport s 1-5 Inoculating Loops & Needles 1-4 BD Viral Transport Systems 1-7 Dehydrated Culture Media BD Culture Media & Ingredients 2-2 BD Media Additives 2-42 Prepared Media BD Fermentation s Prepared Plated Media (PPMs) BD Prepared Slide Media for Urine Diagnostics 3-32 Prepared Tubed Media Prepared Bottled Media BD Prepared Slide Media for Environmental Diagnostics 3-34 Environmental Systems BD GasPak EZ s 4-2 Environmental Chamber 4-6 Direct Testing Systems & Serology Stains & Reagents BD GasPak 100 & 150 Equipment 4-3 BD Rapid Manual Tests 5-2 BD Serology Diagnostics 5-10 & Antigens, Antisera and Adjuvants 6-2 & Stain, Droppers & Indicators 6-19 BD Diagnostic Reagents 6-14 BD Enzymes BD TB Stain Kits & Reagents Identification & Susceptibility Testing Prompt Sytem BD Manual Identification BD Taxo Manual Differentiation BD Sensi-Disc Manual Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Microtrol Quality Control Organisms Blood Culture Systems BD BACTEC 9000 Series: Instruments 8-2 BD Vacutainer Safety Solutions for blood collection & subculturing 8-4 BD BACTEC 9000 Series: Media & Supplement Kit 8-3 BD BACTEC 9000 Series: Accessories 8-5 Mycobacteria Testing Systems BD Mycobacteria Specimen Collection, Processing & Staining 9-2 BD BACTEC MGIT Automated Mycobacteria Testing 9-11 BD Manual Mycobacteria Testing 9-5 Molecular Diagnostics BD ProbeTec ET System BD Probetec ET Consumables BD Affirm VPIII Microbial Identification System BD Diagnostics GeneOhm Data Management Statistics & Epidermiology Indices BD EpiCenter Advance Data Management System BD EpiCenter for BD Phoenix BD EpiCenter for BD BACTEC series BD EpiCenter for BD BACTEC MGIT 960 Alphabetical Index 12-2 Numerical Index

10 Ordering Procedure Trademarks BD, BD Acidicase, BD Affirm, BD BACTEC, BD BACTEC MGIT, BD Bacto (1),, Crystal, BD Biosate, BD BiTek, BD CampyPak, BD CampyPak Plus, BD CampyPouch, BD CDT, BD Cefinase, BD Cellmatics, BD Cell, BD CHROMagar, BD ColorPAC, BD Crystal, BD CrystalSpec, BD CTA Medium, BD CultureSwab (1), BD CultureSwab EZ, BD CultureSwab Plus, BD Culturette, (1), BD Directigen, BD Dispenstirs, BD DispensTube, BD DrySlide (1), BD Enterococcosel, BD Enterotube (2), BD EpiCARE, BD EpiCenter, BD Finger Dab, BD FOS, BD GasPak, BD GasPak EZ, BD GasPak 100, BD GasPak 150, BD GC-Lect, BD Gelysate, BD Hycheck (1), BD IsoVitaleX, BD LINK2, BD LitrePak, BD Luer-Lok, BD Macro-Vue, BD MGIT, BD MicroProbe, BD Mycoflask, BD Mycophil, BD MycoPrep, BD Mycosel, BD Oxi/Ferm (2), BD PANTA, BD Pasco, BD Peds Plus, BD Phoenix, BD Phytone, BD Plastipak, BD Pneumoslide, BD Polypeptone, BD Port-A-Cul, BD ProbeTec, BD Pseudosel, BD Qtest, BD RODAC, BD Select APS, BD Sensi-Disc, BD ssa, BD Stacker, BD Streptocard, BD SWUBE, BD Taxo, BD Thiotone, BD Trichosel, BD Trypticase, BD Vacutainer, BD VZVscan, BD Safety-Lok. Ordering Procedure Specific ordering procedures differ worldwide. Please consult your local office for the appropriate information. Non- Portfolio s Inquiries or requests regarding packaging or product variations not appearing in this product portfolio should be directed to your local BD or Distributor Sales Representative or BD Diagnostic Systems Technical Service. Technical Information and Equipment Repair Specific communication and information procedures for technical information and equipment repair differ worldwide. Please consult your local office for the appropriate information. World Wide Web or or ISO Registration BD Diagnostic Systems products are manufactured in ISO 9000 registered facilities. Specific ISO 9000 registrations per manufacturing facility location include: Madison, Wisconsin, USA ISO 9002 Cayey, Puerto Rico ISO 9002 Sparks, Maryland, USA ISO 9001 Detroit, Michigan, USA ISO 9001 Heidelberg, Germany ISO 9002 (1) Trademark of Difco Laboratories Incorporated, division of Becton, Dickinson and Company (2) Trademark of Becton Dickinson GmbH ATCC is a trademark of the American Type Culture Collection. CHROMagar is a trademark of Dr. A. Rambach. Difco is a trademark of Difco Laboratories, Inc., subsidiary of Becton, Dickinson and Company. GonoGen is a trademark of New HorizonsDiagnostics Corporation. Jembec is a trademark of Miles Scientific. Tween is a trademark of ICI Americas, Inc. Tyvek is a trademark of EI du Pont de Nemours and Company. For specific certificates of ISO registration, please contact your local office. Regulatory Documentation Certificate of Origin and Certificate of Analysis: Use this web site to access documents 24 hours a day, 7 days a week.

11 Collection & Transport Systems BD Collection and Transport products are used for the collection and transport of specimens to the laboratory for recovery of organisms present in the specimens. For over 60 years BD has remained the trusted leader in specimen collection and transport, instilling the care and quality in our products that both patients and clinicians who rely on us have come to expect. As a result, nearly 90 % of all microbiology labs choose BD Collection and Transport products for their needs. BD Specimen Collection & Transport Systems 1-2 BD Difco TM Inoculation Loops & Needles 1-4 Other BD Collection & Transport s 1-5 BD Viral Transport Systems 1-7

12 Collection & Transport Systems BD Specimen Collection & Transport Systems BD CultureSwab, BD CultureSwab EZ & BD CultureSwab PLUS devices BD CultureSwab devices are sterile ready-to-use systems intended for the collection, transport and preservation of clinical specimens for bacteriological examination swabs swabs swabs swabs swabs swabs swabs swabs swabs swabs BD CultureSwab - Cary-Blair Agar (Single Swab) For transport of fecal specimens that possibly contain fastidious and enteric pathogens. Single Swab with Plastic Shaft. BD CultureSwab - Liquid Amies (Single Swab) For throat, vaginal, skin, and wound specimens. Contains a sterile polyurethane foam single swab with Liquid Amies media. Single Swab with Plastic Shaft. BD CultureSwab - Liquid Stuart (Single Swab) For throat, vaginal, skin, and wound specimens. Contains a sterile polyurethane foam single swab with Liquid Stuart media. Single Swab with Plastic Shaft. BD CultureSwab - Liquid Stuart (Double Swab) For throat, vaginal, skin, and wound specimens. Contains a sterile polyurethane foam double swab with Liquid Stuart media. Double Swab with Plastic Shaft. BD CultureSwab - Liquid Stuart (Mini Tip Swab) For male urethral sampling, as well as ear, nose, throat, and eye specimens. Contains a sterile soft aluminum wire swab with Liquid Stuart media. With Soft Aluminum Mini-Tip Swab. BD CultureSwab - Sterile (Single Swab) For general specimen laboratory use. Rayon Swab without Medium in labelled Tube with Plastic Shaft. BD CultureSwab EZ - Sterile (Single Swab) Single Polyurethane Foam Swab without Medium. BD CultureSwab EZ - Sterile (Double Swab) Dual Polyurethane Foam Swab without Medium. BD CultureSwab PLUS - Amies Gel with Charcoal (Single Swab) For throat, urogenital, and wound specimens. Single Swab with Plastic Shaft. BD CultureSwab PLUS - Amies Gel without Charcoal (Single Swab) For throat, vaginal, skin, and wound specimens. Contains a sterile polyurethane foam single swab with Amies gel but no charcoal. Single Swab with Plastic Shaft. 1-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

13 Collection & Transport Systems BD Specimen Collection & Transport Systems BD Port-A-Cul Transport Systems x 20 ml BD Port-A-Cul - Transport Jar (Sterile Pack) 3" sterile pack screw cap jar with 1" wide mouth. Each envelope contains one transport jar (20 ml/jar) for transportation of aerobic and anaerobic samples in a medium containing reducing agents. Best suited for tissue and biopsy specimens. The wide-mouth jar (diameter 2.54 cm) and screw cap allow for easy insertion of a specimen x 11 ml x 11 ml x 5 ml x 5 ml BD Port-A-Cul - Tube BD Port-A-Cul tubes (size D, 11 ml) for Aerobic and Anaerobic Cultures contain a reduced transport medium and are intended for use in maintaining the viability of a wide variety of anaerobic, facultative, and aerobic organisms during transport from the patient to the laboratory. Viability is maintained for up to 72 hours at 20 to 25 C. BD Port-A-Cul - Tube & Swabs (Sterile Pack) Contains a package of 10 envelopes, each containing one size D tube (11 ml/tube) and two swabs for transport of specimens in a reduced solidified holding medium in contrast to other methods in which swabs are placed into fluid media where organisms may be diluted or into an empty tube where organisms may dry out. BD Port-A-Cul - Vial Each envelope contains 10 vials (5 ml/vial) for transportation of aerobic and anaerobic samples in a medium containing reducing agents. BD Port-A-Cul - Vial (Sterile Pack) Package of 10 envelopes, each containing one sterile vial (5 ml/vial) for transportation of aerobic and anaerobic samples in a medium containing reducing agents. BD Vacutainer Anaerobic Specimen Collector BD Vacutainer - Anaerobic Specimen Collector Contains a built in oxygen elimination system for transport of anaerobic specimens. Jembec Modified Thayer Martin Agar Jembec - Modified Thayer-Martin (MTM II) Agar For the isolation and cell culture of pathogenous neisseria (gonococci and meningococci). Includes 10 plates, 10 CO2 tablets, 10 plastic pouches that can be sealed for transport. BD Diagnostics Diagnostic Systems Catalog 2008/

14 Collection & Transport Systems Inoculation Loops & Needles Inoculation Loops & Needles Sterile, disposable inoculating loops & needles do not require flaming. Made from a unique plastic resin for controlled flexibility. They resist cutting the agar surface yet provide sufficient firmness to pick stubborn colonies. Color coded Sterile disposable Inoculating Needle (Violet) Sterile disposable Inoculating Loops - 1 μl (Light Green) high-quality Inoculating Loops are the only disposables with the flexible feel of wire. For maximum control, minimum agar damage, and maximum savings. Sterile disposable Inoculating Loops - 10 μl (Light Blue) 1-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

15 Collection & Transport Systems Other BD Collection & Transport s BD Falcon Sputum Collection System BD Falcon - Sputum Collection Kit Engineered for safer sputum collection, transport and handling the patented BD Falcon Sputum Collection System is designed to help to protect clinicians, laboratorians and uninfected patients from potential sputum pathogens such as mycobacterium. The BD Falcon Sputum Collection System allows for the specimen to be collected directly into a shatter-resistant conical BD Falcon tube, a protective flap then seals off the collection end. The unique hinged design allows for a screw cap to be tightened over the specimen tube without the clinician touching the cap or the collection end of the tube. Ultimately the sputum specimen is delivered to the lab secure and already in the processing tube eliminating the risk inherent when a generic container is used for collection which requires the lab to pour sputum into a separate processing tube. The Kit contains 72 collection systems, including 50 ml conical BD Falcon Tubes. BD Falcon SWUBE Collection & Transport Systems For collection, transport and storage of specimens without added media BD Falcon SWUBE - 15 cm, Single Cotton Swab (Friction Cap) BD Falcon brand cotton-tipped applicators are for specimen collection and transport, and for sanitary equipment and environmental control studies in facilities such as hospitals, food processing plants, restaurants, etc. The BD Falcon SWUBE Applicator is a swab in a tube which facilitates collection, storage, and transport to the laboratory and protects the specimen from contamination. Each BD SWUBE Applicator is individually wrapped. This Friction-Fit Single BD SWUBE Applicator is a single cotton-tipped applicator, approximately 15.2 cm (6") in length, in a 17 x 100 mm plastic tube. Applicator is inserted though the specially designed friction fit cap BD Falcon SWUBE - 15 cm, Single Cotton Swab (Screw Cap) This Screw Cap Single BD SWUBE Applicator is a cotton-tipped applicator, approximately 15.2 cm (6") in length, attached to the screw cap of a 16 x 150 mm clear plastic tube. BD Falcon SWUBE - 15 cm, Double Cotton Swab (Screw Cap) This Double BD SWUBE Applicator contains two cotton-tipped applicators, approximately 15.2 cm (6") in length, attached to the screw cap of a 16 x 150 mm clear plastic tube. BD Falcon SWUBE - 15 cm, Single Polyester Swab (Friction Cap) Reports indicate the recovery of certain organisms, especially beta-hemolytic streptococci, may be improved by collection of specimens with the polyester fiber-tipped applicator. The BD Falcon Single Polyester Fiber-Tipped SWUBE Applicator is approximately 15.2 cm (6") in length. Applicators are sterile and individually packaged in Peel-Apart packages BD Falcon SWUBE - 15 cm, Single Polyester Swab (Screw Cap) The BD Falcon Polyester Fiber-Tipped SWUBE Applicator is a polyester fiber-tipped applicator attached to a screw cap of a 16 x 150 mm clear plastic tube. The screw cap with applicator is interchangeable with the screw cap of the "A" tube in which certain BBL tubed media are supplied. BD SWUBE Applicators are individually wrapped. BD Diagnostics Diagnostic Systems Catalog 2008/

16 Collection & Transport Systems Other BD Collection & Transport s BD Transport Swab for Environmental Monitoring BD Sterile Pack Swabs Maximum sterility with double wrapped packaging, gamma-irradiation, and performancevalidation. Features: Packaged 50 to a box, with 10 single wrapped swabs in each double wrapped pouch Polypropylene tube is pre-filled with a 10 ml rinse solution Simple sampling and transport easy-to-use swab is fixed to the screw cap Complies with the new ISO guidelines Each lot comes with a Certificate of Analysis ATP-free, Dacron swab Saves time, no need to create your own media and swab/solution set in house Convenient and cost-effective solution One year shelf-life, at room temperature storage 1-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

17 Collection & Transport Systems BD Viral Transport Systems BD Viral Transport Systems BD UVT provides on formulation for storage and transprot at 2-25 C, intended for the collection and transport of clinical specimens containing viruses, chlamydiae, mycoplasmas or ureaplasmas from the collection site to the testing laboratory x 3 ml BD Universal Viral Transport Vial BD Universal Viral Transport 3 ml Vial, carton of 50. BD UVT contains: - antimicrobics to suppress bacterial and fungal contaminants - ph indicator to indicate presence of contamination - sucrose, which is a cryoprotectant, enabling storage of viruses and chlamydia when frozen BD Universal Viral Transport Standard Polyester Swabs BD Universal Viral Transport Regular Swabs, sterile polyester-tip with scored plastic shaft, 2 per pouch. BD Universal Viral Transport Polyester Swabs BD Universal Viral Transport Combo Swabs, 1 sterile polyester mini-tip swab with wire/plastic scored shaft and 1 sterile regular swab with scored plastic shaft, 2 per pouch. BD Universal Viral Transport Standard Kit BD Universal Viral Transport Standard Kit, each contains: - 3 ml vial - package of 2 sterile polyester-tip regular swabs with scored plastic shaft BD Universal Viral Transport Combo Kit BD Universal Viral Transport Combo Kit, each contains: - 3 ml vial - package of 1 sterile polyester-tip regular swab with scored plastic shaft and 1 sterile mini-tip swab with wire/plastic scored shaft BD Diagnostics Diagnostic Systems Catalog 2008/

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19 Dehydrated Culture Media BD offers media with a proven record of performance backed by over 180 years of combined BD Difco and expertise and is the only worldwide DCM, Hydrosylates, and prepared media manufacturer with ISO 9000 Certification. BD Tissue Culture, as well as part of the Fermentation product range, which used to be listed in this chapter, are now listed in the BD Bioscience, Advance Bioprocessing Portfolio. BD Culture Media & Ingredients 2-2 BD Fermentation s 2-41 BD Media Addivitives 2-42

20 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Agar-Agar & Agarose: BD Bacto - BBL - Difco Quality kg kg g g g kg kg kg kg kg Agar (Grade A) Agar, Grade A is a high-grade agar, specially processed for microbiological purposes. It is routinely used as a solidifying agent in microbiological media. Agar (Granulated) Used as a solidifying agent for culture media. Carefully monitored for cultural response, solubility and gelation temperature. High quality agar for use in clinics and biotechnology, equivalent to BD BiTek. Suitable for culturing recombinant strains of Escherichia coli (HB 101) and Saccharomyces cerevisiae. May be used for general bacteriological purposes where clarity is not a strict requirement. Agar (Noble) Noble Agar is extensively washed and bleached. This agar should be used for immunodiffusion, some electrophoretic applications, and as a substrate for mammalian and plant tissue culture. Agar, Noble is a solidifying agent that is essentially free of impurities. It is used in electrophoretic and nutritional procedures and in preparing microbiological culture media when increased purity is required. Agar (Purified) Purified Agar in which extraneous matter, pigmented portions and salts are reduced to a minimum. Used for the determination of motility and the growth of anaerobes and microaerophiles. Agar (Technical) Agar, Technical is a solidifying agent used in preparing microbiological culture media. Although Agar, Technical has wider quality control parameters than other bacteriological agars, solubility, gelation temperature and solidity are carefully monitored to permit its use. Agarose Agarose is a complex galactose polysaccharide of near neutral charge. It is specially prepared and is intended mainly for use in gel electrophoresis. European Agar High gel intensity. Gelatin Gelatin is a protein of uniform molecular constitution derived chiefly from the hydrolysis of collagen. Collagens are a class of albuminoids found abundantly in bones, skin, tendon, cartilage and similar tissues of animals. Gelatin is used in culture media to detect gelatin liquefaction by bacteria and as a nitrogen and amino acid source. BD Bacto BD Bacto BD Bacto BD Bacto 2-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

21 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg A-1 Medium Determination of fecal coliforms in water and foods. Acetate Differential Agar Differentiation of members of Shigella genus from Escherichia genus. Actinomycete Isolation Agar Use with added glycerol for isolating and cultivating actinomyces from soil and water. AK Agar No. 2 (Sporulating Agar) Culture medium for the preparation of spore suspensions for use in procedures for the detection of antibiotic residues in milk and dairy products. Anaerobe Broth MIC MIC Susceptibility testing by the broth dilution technique. Anaerobic Agar General purpose medium for anaerobic bacteria. Antibiotic Medium 1 Penassay Seed Agar. Microbiological assay of antibiotics. Antibiotic Medium 2 Penassay Seed Agar. Microbiological assay of antibiotics. Antibiotic Medium 3 Penassay Broth. Antibiotic Medium 4 Yeast Beef Agar Microbiological assay of antibiotics. Antibiotic Medium 5 Streptomycin Assay Agar Microbiological assay of antibiotics. Antibiotic Medium 8 Microbiological assay of antibiotics. Antibiotic Medium 9 Polymyxin Base Agar Microbiological assay of antibiotics. Antibiotic Medium 10 Polymyxin Base Agar Microbiological assay of antibiotics. Antibiotic Medium 11 Erythromycin/Neomycin Agar Microbiological assay of antibiotics. Antibiotic Medium 12 Microbiological assay of antibiotics. BD Diagnostics Diagnostic Systems Catalog 2008/

22 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g g g kg Antibiotic Medium 19 Microbiological assay of antibiotics. APT Agar APT Agar is used for cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content. It is also used for maintaining stock cultures of Weissella (Lactobacillus) viridescens ATCC used in the assay of thiamine. APT Broth Used for culturing Weissella viridescens ATCC used in the assay of thiamine. It is also used for cultivating heterofermentative lactobacilli and other organisms requiring high thiamine content. Azide Blood Agar Base Used for isolating streptococci and staphylococci and, supplemented with blood, for determining hemolytic reactions. Azide Dextrose Broth Used for cultivating streptococci in water and wastewater. B12 Assay Medium Used for determining vitamin B12 concentration by the microbiological assay technique. B12 Culture Agar Used for cultivating Lactobacillus delbrueckii subsp. lactis ATCC 7830 used in the Vitamin B12 Activity Assay. B12 Inoculum Broth Used for preparing the inoculum of L. delbrueckii subsp. lactis ATCC 7830 used in the Vitamin B12 Activity Assay. Baird-Parker Agar Base Used for the preparation of Egg Tellurite Glycine Pyruvate Agar (ETGPA). May also be used for the identification of staphylococci on the basis of their ability to clear egg yolk. Use with EY Tellurite Enrichment (Cat. Nos and ) for the detection and enumeration of coagulase-positive staphylococci from food, skin, soil, air and other materials BCYE Agar Base Buffered Charcoal Yeast Extract (BCYE) Agar Base is used in qualitative procedures for isolation of Legionella species from clinical specimens and nonclinical (environmental) samples. Dehydrated Appearance: Fine to coarse, homogeneous, free of extraneous material. Beef Heart for Infusion 100 g of this Desiccated Powder is equivalent to of fresh beef heart. Used in preparing microbiological culture media. BiGGY Agar BiGGY (Bismuth Sulfite Glucose Glycine Yeast) is a selective and differential medium used in the detection, isolation and presumptive identification of Candida species. Bile Esculin Agar Used to differentiate enterococci and the Streptococcus bovis group from other streptococci. 2-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

23 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g kg g kg kg g kg Biotin Assay Medium For determining biotin concentration by the microbiological assay technique using Lactobacillus plantarum ATCC 8014 as a test organism. Bismuth Sulfite Agar Bismuth Sulfite Agar is a highly selective medium used for isolating Salmonella spp., particularly Salmonella typhi, from food and clinical specimens. Bismuth Sulfite Agar is a modification of the Wilson and Blair formula. Blood Agar Base Infusion medium for isolation and cultivation of a wide variety of microorganisms. Can be used with added blood for cultivation of fastidious microorganisms. Bordet Gengou Agar Base Used with the addition of sterile blood and glycerol for the detection and isolation of B. pertussis and other Bordetella species. The medium is rendered selective by the addition of methicillin. Brain Heart CC Agar With Chloramphenicol and Cycloheximide. Selective medium used for the isolation of pathogenic fungi from specimens heavily contaminated with bacteria and sacrophytic fungi. Brain Heart Infusion BHI is a general-purpose medium used in the cultivation of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. It serves as a base for supplemented media containing 0.1 % agar, Fildes enrichment or 6.5 % sodium chloride. A supplemented pre-reduced formulation in tubes is especially recommended for the cultivation of anaerobes. Brain Heart Infusion Agar For the cultivation of fastidious microorganisms especially fungi and yeasts. For use with added antibiotics in isolating fungi. Brain Heart Infusion Broth, Modified For the cultivation of fastidious organisms; contains modified quantities of the ingredients and contains pancreatic digest of casein instead of pancreatic digest of gelatin. Brain Heart Infusion, Porcine For the cultivation of fastidious microorganisms using porcine as an alternate peptone source. Brain Heart Infusion with PAB and Agar Brain Heart Infusion (BHI) with para-aminobenzoic acid (PAB or PABA) is a medium used for the examination of blood from patients who have received sulfonamide therapy. The addition of agar has been found to improve growth of anaerobes. Brain Heart Infusion with PABA Brain Heart Infusion (BHI) with para-aminobenzoic acid (PAB or PABA) is a medium used for the examination of blood from patients who have received sulfonamide therapy. Brewer Anaerobic Agar Used for cultivating anaerobic and microaerophilic bacteria. BD Bacto BD Bacto BD Bacto BD Bacto BD Diagnostics Diagnostic Systems Catalog 2008/

24 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg Brilliant Green Agar Highly selective medium for the isolation of Salmonella other than Salmonella typhi from feces and other materials. Can be used with Novobiocin Antimicrobic Supplement ( ). Brilliant Green Agar, Modified (Edel-Kampelmacher) Selective Medium for the isolation of Salmonella from water, sewage and foodstuffs. Brilliant Green Bile Agar Used for isolating, differentiating and enumerating coliform bacteria. Brilliant Green Lactose Bile Broth, 2 % Brilliant Green Bile Broth 2 % (Brilliant Green Lactose Bile Broth) is used for the detection of coliform organisms in foods, dairy products, water and wastewater, as well as in other materials of sanitary importance. Brilliant Green Sulfa Agar BG Sulfa Agar. Selective isolation of Salmonella (not S. typhi) from stool and other media after pre-enrichment. Can be used with SBG Sulfa Enrichment ( ). Brucella Agar A culture medium for the cultivation of Brucella organisms. Brucella Broth Used for the cultivation of Brucella species and for the isolation and cultivation of a wide variety of fastidious and nonfastidious microorganisms. Buffered Peptone Water Preenrichment for injured Salmonella species from food specimens to increase recovery. Bushnell-Haas Broth Used for studying microbial utilization of hydrocarbons. Campylobacter Agar Base For use with blood and antibiotics (Campylobacter Antimicrobic Supplement B, Campylobacter Antimicrobic Supplement S or other antibiotics) in isolating and cultivating Campylobacter. Candida BCG Agar Base Candida Bromcresol Green (BCG) Agar is a differential and selective medium used for primary isolation and detection of Candida species from clinical specimens. Cary and Blair Transport Medium Used for collecting, transporting and preserving microbiological specimens, particularly those containing Vibrio cholerae. Casman Agar Base Used for the cultivation of fastidious pathogenic organisms, such as Haemophilus influenzae and Neisseria gonorrhoeae, from clinical specimens. Cetrimide Agar Base - BD Pseudosel Agar Used for the selective isolation and identification of Pseudomonas aeruginosa. 2-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

25 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g kg kg kg kg kg kg kg kg Chapman Stone Medium Used for isolating and differentiating staphylococci based on mannitol fermentation and gelatinase activity. Charcoal Agar Cultivation of fastidious organisms, particularly Bordetella pertussis, for vaccine production and stock culture maintenance. Choline Assay Medium Used for determining choline concentration by the microbiological assay technique. CLED Agar Cystine Lactose-Electrolyte-Deficient Agar. Used for the cultivation of bacteria from urine and for the differentiation of lactose-positive and lactose-negative microorganisms. Coagulase Mannitol Agar Used for the differentiation of Staphylococcus aureus from other species based on coagulase production and mannitol fermentation. Columbia Agar Base A highly nutritious, general-purpose medium for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. Columbia Blood Agar Base Infusion-free basal medium to use with or without blood for the cultivation of fastidious microorganisms. Columbia Blood Agar Base EH Infusion-free basal medium to use with blood for enhanced beta-hemolytic reactions after overnight incubation and for cultivation of fastidious microorganisms, particularly Helicobacter pylori. Columbia Broth Cultivation of fastidious microorganisms. Particularly recommended for blood culture because of its ability to grow a wide range of microorganisms. Columbia CNA Agar Used with blood for the selective isolation of gram positive cocci; contains Colistine and Nalidixin- Acid. Columbia II Agar This is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical material. Cooke Rose Bengal Agar Use with or without Antimicrobic Vial A ( ) for the selective isolation of fungi from environmental and food specimens. Cooked Meat Medium For the cultivation of anaerobic bacteria and maintenance of stock cultures, especially pathogenic clostridia. BBL BD Diagnostics Diagnostic Systems Catalog 2008/

26 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g kg Corn Meal Agar General-purpose medium for the cultivation of fungi. CTA Agar Cystin Trypticase Agar. Primarily used for carbohydrate fermentation tests with corynebacteria and especially for differentiation of C. diphteriae from related species. BD CTA Medium Cystine Trypticase Agar Medium. Culture medium for the maintenance of microorganisms. Also used for the detection of bacterial motility and, with added carbohydrate, for fermentation reactions of fastidious microorganisms, i. e. Neisseria, pneumococci, streptococci and nonsporeforming anaerobes. Cystine Assay Medium Used for determining L-cystine concentration by the microbiological assay technique. Cystine Heart Agar Cystine Tryptic Agar Used for the maintenance of microorganisms, as well as for the detection of bacterial motility and, with added carbohydrate, for fermentation reactions of fastidious microorganisms; i.e., Neisseria, pneumococci, streptococci and nonsporeforming anaerobes. Czapek-Dox Broth Used for cultivating fungi and bacteria capable of using inorganic nitrogen. Czapek Solution Agar Used for cultivating fungi and bacteria capable of using inorganic nitrogen. D/E Neutralizing Agar D/E (Dey/Engley) Neutralizing Agar has the ability to neutralize antimicrobial chemicals and is used for environmental sampling for the detection and enumeration of microorganisms present on surfaces of sanitary importance. D/E Neutralizing Broth Used for the neutralization and testing of antiseptics and disinfectants according to the procedure of Engley and Dey. DCLS Agar DCLS Agar (Desoxycholate Citrate Lactose Sucrose Agar) is a moderately selective culture medium for the isolation of Salmonella and Shigella from fecal specimens. Decarboxylase Base Moeller Use to differentiate bacteria based on ability to decarboxylate amino acids. Decarboxylase Broth Base Moeller Use with added lysine, arginine or ornithine for the differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase and lysine and ornithine decarboxylase. Decarboxylase Medium Base Use with added amino acid in differentiating bacteria based on amino acid decarboxylation. 2-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

27 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg Demi-Fraser Broth Base For use with Fraser Broth Supplement ( ) in selectively and differentially enriching Listeria from foods. Dermatophyte Test Medium Base Dermatophyte Test Medium (DTM) is a selective and differential medium used for the detection and presumptive identification of dermatophytes from clinical and veterinary specimens. Desoxycholate Agar A slightly selective and differential plating medium used for isolating and differentiating gramnegative enteric bacilli. Desoxycholate Citrate Agar A moderately selective and differential plating medium used for isolating enteric bacilli, particularly Salmonella and many Shigella species. Desoxycholate Lactose Agar A slightly selective and differential plating medium used for isolating and differentiating gram-negative enteric bacilli and for enumerating coliforms from water, wastewater, milk and dairy products. Dextrose Agar Used for cultivating a wide variety of microorganisms with or without added blood. Dextrose Broth Used for cultivating fastidious microorganisms and for detecting gas from enteric bacilli. Dextrose Starch Agar Used for cultivating pure cultures of Neisseria gonorrhoeae and other fastidious microorganisms. Dextrose Tryptone Agar Used for cultivating thermophilic flat-sour microorganisms associated with food spoilage. Differential Reinforced Clostridial Agar (DRCA) For enumeration and cultivation of sulfate-reducing clostridia in foods. DNase Test Agar Differential medium used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens. DNase Test Agar with Methyl Green Deoxyribonuclease test for differentiation of S. aureus and S. marcescens from organisms with similar characteristics. DRBC Agar Dichloran Rose Bengal Chloramphenicol Agar supports good growth of yeasts and molds while inhibiting bacteria and the spreading of rapidly growing molds. Dubos Broth Base Dubos Broth Base is used with Dubos Medium Albumin ( ) for rapidly cultivating pure cultures of Mycobacterium tuberculosis. BD Diagnostics Diagnostic Systems Catalog 2008/

28 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg g Dubos Oleic Agar Base Dubos Oleic Agar Base is used with Dubos Oleic Albumin Complex ( ) and penicillin for isolating and determining the susceptibility of M. tuberculosis. EC Medium Differentiation and enumeration of fecal and nonfecal coliforms in water, wastewater, shellfish and foods. EC Medium, Modified Use with Novobiocin Antimicrobic Supplement ( ) for the preenrichment of food samples (meat and poultry products) prior to the detection of E. coli O157:H7. EC Medium with MUG Detection of MUG positive E. coli using a fluorogenic assay in water, food and milk. EE Broth For the isolation of Enterobacteriaceae particularly from foods. EE Broth Mossel Use for selectively enriching and detecting Enterobacteriaceae, particularly from foods. Elliker Broth Elliker Broth, also known as Lactobacilli Broth, is used for cultivating streptococci and lactobacilli, particularly in dairy procedures. Endo Agar A differential and slightly selective culture medium for the detection of coliform and other enteric microorganisms. BD Enterococcosel Agar BD Enterococcosel Agar, a Bile Esculin Agar with Azide, is used for the rapid, selective detection and enumeration of enterococci. BD Enterococcosel Broth BD Enterococcosel Broth, a Bile Esculin Broth with Azide, is recommended for use in the differentiation of enterococci and group D streptococci. Eosin Methylene Blue Agar (Levine) Without Lactose Eosin Methylene Blue Agar, Levine, without Lactose is provided for convenience in genetic studies of enteric bacilli. Eosin Methylene Blue Agar, Modified (Holt-Harris & Teague) A slightly selective and differential medium for the isolation, cultivation and differentiation of gramnegative enteric bacilli from both clinical and nonclinical specimens. Esculin Iron Agar Esculin Iron Agar (EIA substrate) is used for enumerating enterococci from water by membrane filtration based on esculin hydrolysis BD Diagnostics Diagnostic Systems Catalog 2008/2009

29 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality x 10 g g Eugon Agar A general-purpose medium used for cultivating a wide variety of microorganisms. Eugon Agar can be used with or without enrichment. Enriched with blood, Eugon Agar supports the growth of pathogenic fungi including Nocardia, Histoplasma and Blastomyces. With the addition of BD Difco Supplement B ( ), excellent growth of Neisseria, Francisella and Brucella is achieved. The unenriched medium supports rapid growth of lactobacilli associated with cured meat products, dairy products and other foods. Eugon Broth A general-purpose medium used for the cultivation of fastidious and nonfastidious bacteria from a variety of clinical and nonclinical specimens. EVA Broth EVA (Ethyl Violet Azide) Broth is used for detecting and confirming enterococci in water and other specimens as an indication of fecal contamination. FA Buffer (Dried) A phosphate-buffered saline (PBS) which, upon rehydration, yields a 0.85 % NaCI solution buffered to ph 7.2. FA Buffer, Dried is used in preparing: Reactive Control Serum (4+) - Unabsorbed Minimally Reactive Control Serum (1+) Nonreactive Control Serum (N) Nonspecific Staining Control - Unabsorbed BD Bacto g g g kg Fletcher Medium Base Use with sterile normal rabbit serum for isolation, cultivation and maintenance of Leptospira. Folic Acid Assay Medium Used for determining folic acid concentration by the microbiological assay technique. Folic Acid Casei Medium Used for determining folic acid concentration by the microbiological assay technique. Folic AOAC Medium Used for determining folic acid concentration by the microbiological assay technique. Fraser Broth Base Use with Fraser Broth Supplement ( ) for the selective enrichment and detection of Listeria. FTA Hemagglutination Buffer FTA Hemagglutination Buffer (Phosphate Buffered Saline, ph 7.2) is used in the FTA-ABS test and other serological procedures as a diluent and for washing slide preparations. GC Medium Base Used with various additives in isolating and cultivating Neisseria gonorrhoeae and other fastidious microorganisms. Giolitti-Cantoni Broth Base Used for enriching Staphylococcus aureus from foods during isolation procedures. GN Broth Gram Negative Broth; For the selective cultivation of Salmonella and Shigella. BD Diagnostics Diagnostic Systems Catalog 2008/

30 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg g g GN Broth - Hajna Gram Negative Broth, Hajna; Isolation and cultivation of gram-negative organisms. HC Agar Base HC Agar Base, when supplemented with Polysorbate 80, is used for enumerating molds in cosmetic products. Heart Infusion Agar A general-purpose medium used in the cultivation of a wide range of microorganisms from a variety of clinical and nonclinical specimens. Heart Infusion Broth Used for cultivating fastidious microorganisms. Hektoen Enteric Agar A modrately selective medium used in qualitative procedures for the isolation and cultivation of gram-negative enteric microorganisms, especially Shigella, from a variety of clinical and nonclinical specimens. Hemoglobin, Bovine (Freeze-Dried) Hemoglobin products are used in preparing microbiological culture media. Indole Nitrite Medium (BD Trypticase Nitrate Broth) Used for the identification of microorganisms by means of the nitrate reduction and indole tests. Inositol Assay Medium Use for determining inositol concentration by the microbiological assay technique. ISP Medium 1 International Streptomyces Project Tryptone Yeast Extract Broth. ISP Medium 2 International Streptomyces Project Yeast Malt Extract Agar. ISP Medium 4 International Streptomyces Project Inorganic Salts Starch Agar. KF Streptococcus Agar Used with TTC Solution 1 % ( or ) in isolating and enumerating fecal streptococci. KF Streptococcus Broth Used for isolating fecal streptococci. Kligler Iron Agar Used for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to liberate sulfides. Lactobacilli Agar AOAC Used for maintaining stock cultures for microbiological assays of vitamins and amino acids. BD Bacto BD Bacto 2-12 BD Diagnostics Diagnostic Systems Catalog 2008/2009

31 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g kg kg g kg kg kg kg g Lactobacilli Broth AOAC Used for preparing inocula for microbiological assays of vitamins and amino acids. Lactobacilli MRS Agar For use in the isolation, enumeration and cultivation of Lactobacilli species. Lactobacilli MRS Broth For use in the isolation, enumeration and cultivation of Lactobacillus species. Lactose Broth Used for detection of the presence of coliform organisms, as a pre-enrichment broth for salmonellae and in the study of lactose fermentation of bacteria in general. Lactose Monohydrate Used as a filler or diluent in tablets, capsules and lyophilized products. Lactose Peptone Broth Detection of coliform organisms in water. Lauryl Sulfate Broth Known also as Lauryl Sulfate Tryptose (LST) Broth. Used for the detection of coliform organisms in materials of sanitary importance. Lauryl Sulfate Broth with MUG Known also as Lauryl Sulfate Tryptose Broth with MUG (LST-MUG). Used for the detection of Escherichia coli in water, food and dairy samples by a fluorogenic procedure. Lauryl Tryptose Broth Lauryl Sulfate Broth Medium. For the detection of coliform organisms in water and wastewater. Lauryl Tryptose Broth with MUG With 4-Mehtylumbelliferyl-ß-D-glucuronid for fluorogenic testing. Use for the fluorogenic detection of E. coli in water, food and milk. LBS Agar Lactobacillus Selection Agar is used for the selective isolation and enumeration of lactobacilli. LBS Broth Lactobacillus Selection Broth. Use for the isolation and cultivation of Lactobacilli species. Leptospira Medium Base EMJH Use with Leptospira Enrichment EMJH ( ) to cultivate and maintain Leptospira. Letheen Agar Used to inactivate quarternary ammonium compounds and other preservatives when determining the number of bacteria present in cosmetics and other materials. BD Diagnostics Diagnostic Systems Catalog 2008/

32 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg Letheen Agar, Modified Used for the microbiological testing of cosmetics. Letheen Broth Use for determining the phenol coefficient of cationic surface-active materials. Letheen Broth, Modified Microbiological evaluation of cosmetics: inactivates preservative agents. Levine EMB Agar Levine Eosin Methylene Blue Agar is a slightly selective and differential plating medium for the isolation of gram-negative enteric bacteria. Listeria Enrichment Broth Selective enrichment for L. monocytogenes from nondairy and processed food products. Listeria Enrichment Broth, Modified Used for selectively enriching Listeria from raw and pasteurized milk according to the International Dairy Federation (IDF). Litmus Milk Litmus Milk is used for the maintenance of lactic acid bacteria and as a differential medium for determining the action of bacteria on milk. Liver (Desiccated Powder) Desiccated powder of beef liver. Liver is prepared from large quantities of carefully trimmed fresh beef liver. Liver is a desiccated powder of beef liver. The nutritive factors of fresh liver tissue are retained in infusion prepared from Liver. Liver is used as a source of nitrogen, amino acids and vitamins in microbiological culture media. The reducing substances contained in liver create an anaerobic environment, necessary to support the growth of anaerobes. One hundred thirty-five (135) grams of desiccated Liver are equivalent to rams of fresh liver. Liver Infusion Agar Cultivation of Brucella, anaerobes, and other pathogens. Liver Infusion Broth Use for cultivating a variety of organisms, particularly Brucella and anaerobes. Liver Veal Agar For the cultivation of anaerobic bacteria. Loeffler Blood Serum Use for cultivating Corynebacterium diphteriae from clinical specimens and in pure culture. Can also be used for demonstrating pigment production and proteolysis. Loewenstein Medium Base Media for the growth and recovery of Mycobacteria. Can be used with glycerol to prepare a variety of coagulated egg media. LPM Agar Base Used with Listeria Selective Supplement ( ) for isolating and cultivating Listeria monocytogenes BD Diagnostics Diagnostic Systems Catalog 2008/2009

33 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g g g g g Lysine Decarboxylase Broth Used for differentiating microorganisms based on lysine decarboxylation. Lysine Iron Agar Used for the differentiation of enteric organisms based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulfide. M Broth BD Bacto M Broth is used for cultivating Salmonella in foods and feeds by the accelerated enrichment serology (ES) procedure. M17 Agar Use for the cultivation and isolation of mesophilic lactic streptococci and for the selection of Streptococcus thermophilus from yogurt, cheese starters and other dairy products. M17 Broth Used for isolating lactic streptococci from yogurt, cheese starters and other dairy products. m Endo Agar LES LES = Lawrence Experimental Station. Usee for the enumeration of coliforms in water by the membrane filter technique. m Endo Broth MF Used for enumerating coliform organisms in water by membrane filtration. m Enterococcus Agar m Enterococcus Agar, also referred to as m Azide Agar, is used for isolating and enumerating enterococci in water and other materials by membrane filtration or pour plate technique. m FC Agar Use with Rosolic Acid. For detection and enumeration of fecal coliforms by the membrane filter technique at elevated temperatures. m FC Broth Base Use with Rosolic Acid. For detection and enumeration of fecal coliforms by the membrane filter technique at elevated temperatures. m HPC Agar Used for enumerating heterotrophic organisms in treated potable water and other water samples with low counts by membrane filtration. m Plate Count Broth Used for enumerating microorganisms by membrane filtration. m Staphylococcus Broth Used for isolating staphylococci by the membrane filtration technique. m TEC Agar m TEC Agar is used for isolating, differentiating and rapidly enumerating thermotolerant Escherichia coli from water by membrane filtration and an in situ urease test. BD Bacto BD Diagnostics Diagnostic Systems Catalog 2008/

34 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg kg kg m TGE Broth m TGE Broth, also known as membrane Tryptone Glucose Extract Broth, is used for enumerating microorganisms by membrane filtration. MacConkey Agar For differential isolation of enteric bacilli based on lactose fermentation. MacConkey Agar Base Prepared without carbohydrates for coliform fermentation studies. MacConkey Agar without Crystal Violet Less selective than MacConkey Agar, to permit growth of staphylococci and enterococci. MacConkey Agar without Crystal Violet or Salt For the isolation and differentiation of enteric organisms. MacConkey Agar without Salt Restricts the swarming of most Proteus sp., facilitating gram-negative bacilli isolation. MacConkey Broth Selective medium for detection of gram-negative, lactose fermenting coliforms in water and foods. MacConkey II Agar Isolation of gram-negative enteric bacilli from specimens that may contain swarming strains of Proteus. MacConkey Sorbitol Agar Isolation and differentiation of enteropathogenic E. coli serotypes. Malonate Broth Differentiation of Enterobacter from Escherichia on the basis of malonate utilization. Malonate Broth, Ewing Modified Use for the differentiation of coliforms and other enteric organisms. Malonate Broth, Modified Differentiation of Enterobacteriaceae on the basis of malonate utilization. Malt Agar Use for isolating and cultivating yeasts and molds from food, and for cultivating yeast and mold stock cultures. Malt Extract Agar Isolation, detection and enumeration of yeasts and molds BD Diagnostics Diagnostic Systems Catalog 2008/2009

35 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg g kg Malt Extract Broth Used for cultivating yeasts and molds. Mannitol Salt Agar Used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials. Marine Agar 2216 Isolation, cultivation and enumeration of heterotrophic marine bacteria. Marine Broth 2216 For the cultivation of heterotrophic marine bacteria. Maximum Recovery Diluent Isotonic diluent containing a low level of peptone used for maintaining the viability of organisms during dilution procedures. McClung Toabe Agar Base Use with Egg Yolk Enrichment 50 % (Cat. Nos and ) for the detection and isolation of Clostridium perfringens from foods based on the lecithinase reaction. M-Green Yeast and Mold Broth Used for the detection of fungi in the routine analysis of beverages. MI Agar MI Agar* is a chromogenic/fluorogenic medium used to detect and enumerate Escherichia coli and total coliforms in drinking water by the membrane filtration technique. It conforms with U.S. Environmental Protection Agency (USEPA) Approved Method 1604: Total oliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium). Micro Assay Culture Agar For the cultivation of lactobacilli and other organisms used in the microbiological analysis of vitamins and amino acids. Micro Inoculum Broth Used for preparing the inoculum of lactobacilli and other microorganisms used in microbiological assays of vitamins and amino acids. Microbial Content Test Agar Microbial Content Test Agar = Tryptic Soy Agar with Polysorbate 80, is recommended for the detection and enumeration of microorganisms present on surfaces of sanitary importance. Middlebrook 7H10 Agar Used for the isolation, cultivation and susceptibility testing of mycobacteria. Middlebrook OADC Enrichment (Cat. Nos or or ) and Glycerol must be added; the complete Prepared Plated Medium (with OADC) is available under , Middlebrook and Cohn 7H10 Agar (see chapter 4-2 of this catalogue). BD Diagnostics Diagnostic Systems Catalog 2008/

36 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality Middlebrook 7H11 Agar Base Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Middlebrook OADC Enrichment (Cat. Nos or or ) and Glycerol must be added to the Agar Base before use Middlebrook 7H9 Broth For cultivation of mycobacteria and preparation of tubercle emulsion for susceptibility testing. Middlebrook ADC Enrichment (Cat. Nos or ) and Glycerol or Tween 80 must be added to the Broth before use MIL Medium Used for differentiating Enterobacteriaceae based on motility, lysine decarboxylation, lysine deamination and indole production. Milk Agar Recommended by the British Standards Institute and the International Dairy Federation for the enumeration of microorganisms in liquid milk, ice cream, dried milk and whey. Minerals Modified Glutamate Broth Defined glutamate medium for the enumeration of coliform organisms in water. Minimal Agar Davis Used for isolating and characterizing nutritional mutants of Escherichia coli. Minimal Broth Davis without Dextrose Use with added dextrose to isolate and characterize nutritional mutants of Escherichia coli and Bacillus subtilis. MIO Medium Motility Indole Ornithine (MIO) Medium is used to demonstrate motility, indole production and ornithine decarboxylase activity for the differentiation of Enterobacteriaceae. Mitis Salivarius Agar Mitis Salivarius Agar is used with Tellurite Solution 1 % ( ) in isolating Streptococcus mitis, S. salivarius and enterococci, particularly from grossly contaminated specimens. Motility GI Medium Semisolid gelatin heart infusion medium for detecting motility of microorganisms and for separating organisms in their motile phase. Motility Test Medium For the determination of motility of gram-negative enteric bacilli. M-PA-C Agar For the selective recovery and enumeration of Pseudomonas aeruginosa from water. MR-VP Broth MR-VP Broth (Methyl Red Voges-Proskauer Broth, also known as Buffered Peptone- Glucose Broth) are used for the differentiation of bacteria by means of the methyl red and Voges- Proskauer reactions BD Diagnostics Diagnostic Systems Catalog 2008/2009

37 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg kg kg MR-VP Medium Methyl Red Voges-Proskauer Medium. Use for differentiating coliform organisms based on the methyl red and Voges-Proskauer tests. Mueller Hinton Broth Mueller Hinton Broth (Not cation-adjusted). A general purpose medium that may be used in the cultivation of a wide variety of fastidious and nonfastidious microorganisms. This medium is not supplemented with calcium or magnesium ions. Mueller Hinton II Agar Recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method comes in a BD LitrePak (20 x 1 l). Mueller Hinton II Broth Mueller Hinton II Broth (Cation-Adjusted). For use in quantitative procedures for susceptibility testing of rapidly-growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It is formulated to have a low thymine and thymidine content and is adjusted to the calcium and magnesium ion concentrations recommended in CLSI (formerly NCCLS) standard M7. Mueller Hinton Medium Mueller Hinton Agar is recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method,1-3 as standardized by the National Committee for Clinical Laboratory Standards (NCCLS, now CLSI). Muller Kauffmann Tetrathionate Broth Base Use to enrich Salmonella from water, foodstuffs and fecal samples prior to selective isolation. Mycobacteria 7H11 Agar Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Used with Middlebrook OADC Enrichment (Cat. Nos or or ) and Glycerol Mycological Agar Mycological media are used for the cultivation and maintenance of fungi, for the demonstration of chromogenesis and for obtaining yeast and mold counts. BD Mycophil Agar BD Mycophil Agar is a nonselective medium of value in general work with yeasts and molds rather than for isolation from materials possessing mixed flora. It is often desirable to use these media in parallel with selective media as some of the selective agents are inhibitory for certain fungi. BD Mycophil Agar with Low ph BD Mycophil Agar with Low ph has had its base adjusted to approximately ph 4.7, which obviates the need for ph adjustment with lactic or tartaric acids in the laboratory. It also differs from BD Mycophil Agar in that an additional 2 g/l of agar has been incorporated so that the medium may be sterilized and remelted without losing its ability to solidify. BD Diagnostics Diagnostic Systems Catalog 2008/

38 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality Mycoplasma Agar Base (PPLO Agar) PPLO (Pleuropneumonia-like organism)(mycoplasma) agars, when supplemented with nutritive enrichments (e.g. Mycoplasma Supplement, or Mycoplasma Enrichment without Penicillin, ), are used for isolating and cultivating Mycoplasma. Mycoplasma Broth Base (Frey) Used for the cultivation of avian mycoplasmas. Use with Mycoplasma Supplement ( ) or Mycoplasma Enrichment without Penicillin ( ) for isolating and cultivating Mycoplasma Mycoplasma Broth Base (PPLO Broth Base) Also known as PPLO (Pleuropneumonia-like organism) Broth Base. Basal medium that contains no Crystal Violet and is used in the preparation of media for cultivation of Mycoplasma. Use with Mycoplasma Supplement ( ) or Mycoplasma Enrichment without Penicillin ( ) for isolating and cultivating Mycoplasma g g g kg BD Mycosel Agar A highly selective medium containing cycloheximide and chloramphenicol. It is recommended for the isolation of pathogenic fungi from materials having a large amount of flora of other fungi and bacteria. MYP Agar MYP Agar is used with Egg Yolk Enrichment 50% (Cat. Nos and ) and Antimicrobic Vial P ( ) for enumerating Bacillus cereus from foods. Neutralizing Buffer Recommended for detection of microorganisms found on dairy and food equipment disinfected with chlorine or quaternary ammonium compounds. Niacin Assay Medium Microbiological assay of niacin. Use to determine niacine concentration by the microbiological assay technique. NIH Thioglycollate Broth (USP Alternative Thioglycollate Medium) NIH Thioglycollate Broth and Sterility Test Broth, which are the USP Alternative Thioglycollate Medium, are Fluid Thioglycollate Medium without the agar or indicator components. They are used for the same sterility test procedures except that anaerobic incubation is recommended rather than aerobic incubation. They also meet the requirements of the USP growth promotion test. Nitrate Broth Recommended as an aid in the identification of aerobic and facultative anaerobic gram-negative microorganisms by means of the nitrate reduction test. Nutrient Agar General purpose medium for the cultivation and enumeration of a wide variety of microorganisms in water, sewage, feces and other materials. Nutrient Agar (1.5 %) Use to cultivate a variety of microorganisms. Can be used with the addition of blood or other enrichment for the cultivation of fastidious microorganisms BD Diagnostics Diagnostic Systems Catalog 2008/2009

39 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g g kg kg g g Nutrient Agar (ph 6.0) General purpose medium for cultivation of microorganisms requiring a slightly acidic ph. Nutrient Agar with MUG Fluorogenic assay for the detection and enumeration of E. Coli in water. Nutrient Broth Use for the cultivation of many species of nonfastidious microorganisms. Nutrient Gelatin Used for the detection of gelatin liquefaction by microbial species. Oatmeal Agar Use for cultivating fungi, particularly for macrospore formation. OF Basal Medium OF (Oxidation Fermentation) media are used for the determination of oxidative and fermentative metabolism of carbohydrates by gram-negative rods on the basis of acid reaction in either the open or closed system. OGYE Agar Base Oxytetracycline Glucose Yeast Extract Agar Base. Orange Serum Agar Cultivation of aciduric microorganisms, particularly those associated with spoilage of citrus products. Oxford Medium Base Oxford Medium Base is used with Modified Oxford Antimicrobic Supplement ( ) for isolating and differentiating Listeria monocytogenes. PALCAM Medium Base PALCAM Medium Base is used with PALCAM Antimicrobic Supplement ( ) in isolating and cultivating Listeria, particularly from foods and milk products. Pantothenate Assay Medium Microbiological assay of pantothenate. Use to determine the concentration of pantothenic acid and its salts by the microbiological assay technique. Pantothenate Medium AOAC Microbiological assay of pantothenate. Use for determining the concentration of pantothenic acid and pantothenate by the microbiological assay technique. Peptone Iron Agar Use as an indicator of hydrogen sulfide production by microorganisms. Peptone Water Minimal medium for cultivation of non-fastidious organisms, for studying carbohydrate fermentation patterns, and for performing the indole test. BD Diagnostics Diagnostic Systems Catalog 2008/

40 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g kg g kg Phenol Red Agar Base Use with added carbohydrates in differentiating pure cultures of microorganisms based on fermentation reactions. Phenol Red Broth Base Use with added carbohydrates for the accurate determination of fermentation reactions in the differentiation of microorganisms. Phenol Red Dextrose Broth Determination of the ability of microorganisms to ferment dextrose. Phenol Red Lactose Broth Determination of the ability of microorganisms to ferment lactose. Phenol Red Mannitol Agar Used for differentiating pure cultures of bacteria based on mannitol fermentation reactions. Phenol Red Mannitol Broth Used to measure the ability of an organism to ferment mannitol. Phenol Red Sucrose Broth Used for determining the ability of microorganisms to ferment sucrose. Phenylalanine Agar Used for the differentiation of enteric bacilli on the basis of their ability to produce phenylpyruvic acid by oxidative deamination. Phenylethyl Alcohol Agar Selective medium for the isolation of gram-positive organisms, particularly gram-positive cocci, from specimens of mixed gram-positive and gram-negative flora. BD Phytone Yeast Extract Agar Use for the selective isolation of dermatophytes, particularly Trichophyton verrucosum, and other pathogenic fungi from routine clinical specimens. Plate Count Agar Used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance. Potato Dextrose Agar Use for culturing yeasts and molds from food and dairy products. Potato Dextrose Broth Fot the cultivation of yeasts and molds. Potato Infusion Agar Use to cultivate Brucella, especially in mass cultivation procedures BD Diagnostics Diagnostic Systems Catalog 2008/2009

41 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality PPLO Agar (Mycoplasma Agar) PM Indicator Agar. Penicillin in Milk Assay. Use with Mycoplasma Supplement ( ) or with Mycoplasma Enrichment without Penicillin ( ) for isolating and cultivating Mycoplasma g g kg PPLO Broth (Mycoplasma Broth) Use with Mycoplasma Supplement ( ) or Mycoplasma Enrichment without Penicillin ( ) for isolating and cultivating Mycoplasma. Presence-Absence Broth One step method for presumptive identification of coliforms in treated water from water treatment plants or distribution systems. Proteose No. 3 Agar Use with added enrichment for the isolation and cultivation of Neisseria and Haemophilus. When enriched with Hemoglobin and Supplement B ( ) Proteose No. 3 Agar recovers gonococci in a manner comparable to more complex media, ranking only slightly lower than GC media at 24 hours. The growth rate of Neisseria and Haemophilus spp. may be improved with the addition of 1% Supplement B or VX (Cat. Nos and ), which provide the growth factors glutamine and cocarboxylase. Pseudomonas Agar F Pseudomonas Agar F, also known as Flo Agar, is used for the enhancement of fluorescin production. Pseudomonas Agar P Pseudomonas Agar P, also known as Tech Agar, is used for the enhancement of pyocyanin production by Pseudomonas. Pseudomonas Isolation Agar Use with added Glycerol in isolating Pseudomonas and in differentiating Pseudomonas aeruginosa from other pseudomonas based on pigment formation. Purple Agar Base Use with added carbohydrate in differentiating pure cultures of bacteria, particularly of enteric organisms, based on fermentation reactions. Purple Broth Base Use with added carbohydrate in differentiating pure cultures of bacteria, particularly of enteric organisms, based on fermentation reactions. Pyridoxine Y Medium Used for determining pyridoxine concentration by the microbiological assay technique. R2A Agar Used for enumerating heterotrophic organisms in treated potable water. Raka-Ray No. 3 Medium Recommended for the isolation of lactic acid bacteria encountered in the beer-brewing process. BD Diagnostics Diagnostic Systems Catalog 2008/

42 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality g g kg g kg kg kg kg Rappaport-Vassiliadis Medium, Modified (Semisolid) Use with Novobiocin Antimicrobic Supplement ( ) for the rapid detection of motile Salmonella in feces and food products. Rappaport-Vassiliadis R10 Broth Selective enrichment of Salmonella from meat and dairy products, feces and sewage polluted water and other materials. Regan-Lowe Charcoal Agar Base A selective medium used for isolation of Bordetella pertussis from clinical specimens. Reinforced Clostridial Medium (RCM) Use for the cultivation and enumeration of anaerobes, particularly Clostridia, and other species of bacteria from foods and clinical specimens. Riboflavin Assay Medium Use for determining riboflavin concentration by the microbiological assay technique. Rice Extract Agar Use for the promotion of chlamydospore formation by Candida albicans and C. stellatoidea as a means of differentiating them from other Candida species. Rogosa SL Agar Use for the selective cultivation of oral, vaginal and fecal lactobacilli. Rogosa SL Broth Use for the selective cultivation of oral, vaginal and fecal lactobacilli. Rose Bengal Agar Base Use with Rose Bengal Antimicrobic Supplement ( ) for selective isolation and enumeration of yeasts and molds from foods, dairy products and the environment. Sabouraud Agar, Modified Used in qualitative procedures for cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. Sabouraud Brain Heart Infusion Agar Base Use with chloromycetin and blood (optional) in cultivation and isolation of pathogenic fungi. Sabouraud Dextrose Agar Sabouraud Dextrose Agar is used in qualitative procedures for cultivation of pathogenic and nonpathogenic fungi, particularly dermatophytes. The medium is rendered more selective for fungi by the addition of antimicrobics. Sabouraud Dextrose Broth Used for cultivation of yeasts, molds and aciduric microorganisms BD Diagnostics Diagnostic Systems Catalog 2008/2009

43 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg g Sabouraud Maltose Agar Modification of Sabouraud Dextrose Agar (maltose substituted for dextrose) used for the cultivation of yeasts, molds and aciduric microorganisms. With 4 % Maltose, ph 5,6. Sabouraud Maltose Broth Modification of Sabouraud Dextrose Broth (maltose substituted for dextrose) used for the cultivation of yeasts, molds and aciduric microorganisms. With 4 % Maltose, ph 5,6. Sabouraud Medium, Fluid Use for cultivating yeasts, molds and aciduric microorganisms and for detecting yeasts and molds in normally sterile materials. Salmonella Shigella Agar SS Agar. Differentially selective medium for the isolation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. Not recommended for the primary isolation of Shigella. Schaedler Agar Use with or without blood for the cultivation and enumeration of anaerobic and aerobic microorganisms. Schaedler Broth Use for cultivating anaerobic and aerobic microorganisms with or without added blood or enrichment. Selenite Broth Used as an enrichment medium for the isolation of Salmonella from feces, urine, water, foods and other materials of sanitary importance. Selenite Cystine Broth Used as a selective enrichment medium for the isolation of Salmonella from feces, foods, pharmaceutical articles, water and other materials of sanitary importance. SF Medium SF (Streptococcus Faecalis) Medium is used for the differentiation of Enterococcus species from the Streptococcus bovis group and other streptococci. SFP Agar Base Shahidi Ferguson Perfringens Agar Base. TSC Agar Base. For detection and enumeration of C. perfringens in foods use with: Egg Yolk Enrichment 50 % (Cat. Nos and ) and Antimicrobic Vial P ( ) and Antimicrobic Vial K ( ) SIM Medium Sulfide Indole Motility Medium. Use for differentiating Salmonella and Shigella species based on hydrogen sulfide production, indole fermentation and motility. Simmons Citrate Agar Differentiation and identification of gram-negative bacteria based on citrate utilization. BD Diagnostics Diagnostic Systems Catalog 2008/

44 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg Skim Milk Soluble, spray-dried skim milk. When prepared in a 10 % solution, it is equivalent to fresh skim milk. Use for preparing microbiological culture media and for differentiating organisms based on coagulation and proteolysis of casein. Soluble Starch Soluble starch improves growth response. It provides starch for hydrolysis, detoxification of metabolic byproducts and as a carbon source. Special Yeast and Mold Medium Used for isolating and cultivating yeasts and molds. Spirit Blue Agar Use with Lipase Reagent ( ) or other lipid source for the enumeration and detection of lipolytic microorganisms. SPS Agar Sulfite Polymyxin Sulfadiazine Agar is used for the detection and enumeration of Clostridium perfringens in foods and other materials. Standard Methods Agar Also known as Plate Count Agar. Use for the enumeration of bacteria in water, wastewater, food and dairy products. Also recommended as a general plating medium for determining bacterial populations. Standard Methods Agar with Lecithin and Polysorbate 80 Used for the detection and enumeration of microorganisms present on surfaces that are of sanitary importance. Lecithin and Polysorbate 80 neutralize residual disinfectants from collection sites. Staphylococcus Medium 110 Isolation and differentiation of pathogenic strains of Staphylococcus based on mannitol fermentation, pigment formation and gelatinase activity. Starch Agar Use for cultivating microorganisms being tested for starch hydrolysis. Stock Culture Agar Semisolid medium for maintaining stock cultures of bacteria, particularly streptococci. Sugar Free Agar For the detection and enumeration of organisms in butter and other processed dairy products. Sulfite Agar Sulfite Agar is used for detecting thermophilic, H2S-producing anaerobes, particularly in foods. Synthetic Broth AOAC Use to maintain disinfectant test cultures. Contains all the nutrients essential for growth of the test cultures used to determine the phenol coefficients of disinfectants BD Diagnostics Diagnostic Systems Catalog 2008/2009

45 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg g g TAT Broth Base TAT (Tryptone-Azolectin-Tween ) Broth Base with the addition of polysorbate 20 is recommended for testing for the presence of microorganisms in viscous materials, such as salves or ointments. It is especially adapted to the testing of cosmetics. TCBS Agar Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS Agar) is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens. Tellurite Glycine Agar Use with Chapman Tellurite Solution 1 % ( ) for the selective isolation of coagulase positive staphylococci. Tetrathionate Broth Base Use with Iodine Solution ( ) as a selective enrichment medium for the isolation of Salmonella from feces, urine, foods and other materials of sanitary importance. Tetrathionate Broth Base - Hajna (TT Broth Base, Hajna) Selective enrichment for Salmonella from food and dairy products prior to isolation procedures. Thermoacidurans Agar Isolation and cultivation of Bacillus coagulans (Bacillus thermoacidurans) from foods. Thiamine Assay Medium Used for determining thiamine concentration by the microbiological assay technique using Lactobacillus fermentum ATCC Thiamine Assay Medium LV Used for determining thiamine concentration by the microbiological assay technique using Weissella (Lactobacillus) viridescens ATCC Thioglycollate Medium without Dextrose Detection of a variety of microorganisms in normally sterile materials, especially those containing mercurial preservatives. May be used with added carbohydrates for fermentation studies. Thioglycollate Medium without Dextrose or Indicator Detection of a variety of microorganisms in normally sterile materials, especially those containing mercurial preservatives. May be used with added carbohydrates for fermentation studies. Thioglycollate Medium without Indicator Detection of a variety of microorganisms in normally sterile materials, especially those containing mercurial preservatives. Suitable for fermentation studies when no oxidation-reduction indicator is required. Thioglycollate Medium without Indicator - 135C Enriched general-purpose medium for the recovery of a wide variety of microorganisms, particularly obligate anaerobes, from clinical specimens and other materials. Thioglycollate Medium, Brewer Modified Use for the cultivation of obligate anaerobes, microaerophiles and facultative organisms. BD Diagnostics Diagnostic Systems Catalog 2008/

46 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg g kg kg kg kg kg kg Thioglycollate Medium, Fluid FTM is used to detect microorganisms in normally sterile materials. Thioglycollate Medium, Fluid with Beef Extract Use to cultivate microorganisms from normally sterile biological products. Thioglycollate Medium, Fluid without Dextrose or Eh Indicator Use for fermentation studies, especially with anaerobic organisms. Thiol Broth Use for cultivating organisms from body fluids and other materials containing penicillin, streptomycin or sulfonamides. Tinsdale Agar Base Tinsdale Agar Base is used with Tinsdale Enrichment Desiccated ( ) in isolating and differentiating Corynebacterium diphtheriae. Todd Hewitt Broth General-purpose medium used for the cultivation of group A streptococci, pneumococci and other fastidious organisms or as a blood culture medium. Primarily used for the cultivation of group A streptococci prior to serological typing. Tomato Juice Agar Cultivation and enumeration of Lactobacillus species, especially Lactobacillus acidophilus. Tomato Juice Agar (Special) Tomato Juice Agar Special is used for cultivating and enumerating lactobacilli and other acidophilic microorganisms from saliva and other specimens. Tomato Juice Broth Used in the cultivation of yeasts and other aciduric microorganisms. Transport Medium (Stuart, Toshach and Patsula) Use for the transportation of swab specimens for the recovery of a wide variety of microorganisms, including gonococci. Trichophyton Agar 1 Trichophyton Agars are differential media used in the presumptive identification of Trichophyton species based on nutritional requirements. Contains Dextrose. Trichophyton Agar 2 Contains Inositol and Dextrose. Trichophyton Agar 3 Contains Inositol, Thiamine and Dextrose. BD Bacto BD Bacto BD Bacto 2-28 BD Diagnostics Diagnostic Systems Catalog 2008/2009

47 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg g kg kg kg kg kg kg Trichophyton Agar 4 Contains Thiamine and Dextrose. Trichophyton Agar 6 Contains Ammonium Nitrate and Dextrose. Trichophyton Agar 7 Contains Histidine. BD Trichosel Broth, Modified For the isolation and cultivation of Trichomonas species. Triple Sugar Iron Agar Triple Sugar Iron Agar (TSI Agar) is used for the differentiation of gram-negative enteric bacilli based on carbohydrate fermentation and the production of hydrogen sulfide. Tryptic Soy Agar TSA. Soybean-Casein Digest Agar Medium, USP. Cultivation and isolation of a variety of fastidious and nonfastidious organisms. Use with blood in determining hemolytic reactions. Tryptic Soy Blood Agar Base EH Use with blood in isolating and cultivating fastidious microorganisms from specimens where clear and distinct hemolytic reactions are of prime importance. Tryptic Soy Blood Agar Base No. 2 Used with blood for improved beta-hemolytic reactions and for the cultivation of fastidious microorganisms. Tryptic Soy Broth TSB. Soybean-Casein Digest Medium, USP. Fluid Soybean-Casein Digest Medium. General purpose medium for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and non clinical specimens. BD Select APS - Tryptic Soy Broth Alternative Protein Source obtained from animal-free components. BD Select APS - Tryptic Soy Broth (Sterile) Alternative Protein Source obtained from animal-free components. Serile, Gamma-Irradiated. Tryptic Soy Broth without Dextrose Tryptic Soy Broth without Dextrose, a low carbohydrate formulation of Tryptic Soy Broth, is used for cultivating fastidious and nonfastidious microorganisms. BD Trypticase Soy Agar Used for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes. BD Trypticase Soy Agar, Modified (TSA II) Use with added blood for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. BD Bacto BD Bacto BD Bacto BD Bacto BD Bacto BD Diagnostics Diagnostic Systems Catalog 2008/

48 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg kg kg kg kg BD Trypticase Soy Agar with Lecithin and Polysorbate 80 Recommended for the detection and enumeration of microorganisms present on surfaces of sanitary importance. BD Trypticase Soy Broth General purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens. BD Trypticase Soy Broth (Sterile) Tryptone Glucose Extract Agar Used for cultivating and enumerating microorganisms in water and dairy products. Tryptone Water For detecting Escherichia coli in food and water samples on the basis of indole production. Tryptose Agar Cultivation of Brucella and a large variety of pathogenic organisms. Tryptose Blood Agar Base Infusion free medium used with blood for the isolation and cultivation of fastidious microorganisms as well as the determination of hemolytic reactions. Tryptose Broth Cultivation of Brucella and a variety of pathogenic microorganisms. Tryptose Phosphate Broth Infusion-free buffered medium for the cultivation of fastidious, pathogenic microorganisms. TSN Agar TSN (BD Trypticase Sulfite Neomycin) Agar is used for the selective isolation of Clostridium perfringens. Universal Beer Agar Universal Beer Agar (UBA Medium) is used for cultivating microorganisms of significance in the brewing industry. Universal Preenrichment Broth Used for recovering sublethally injured Salmonella and Listeria from food products. Traditional methods for recovering Salmonella and Listeria from food products require separate preenrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. Urea Agar Base Used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Use with BD Bacto Agar (Cat. Nos , , , ) for differentiating microorganisms based on urease activity. Urea Broth Use for differentiating microorganisms, particularly Proteus species, based on urease production. BD Bacto BD Bacto 2-30 BD Diagnostics Diagnostic Systems Catalog 2008/2009

49 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg g UVM Modified Listeria Enrichment Broth Use as a selective enrichment for the rapid isolation of Listeria monocytogenes. Veal Infusion Agar Cultivation of fastidious organisms with or without added enrichment. Veal Infusion Broth Cultivation of fastidious organisms. Violet Red Bile Agar Used for enumerating coliform organisms in dairy products. Violet Red Bile Agar with MUG Selective isolation of coliform bacteria and fluorogenic detection of Escherichia coli from food and dairy products. Violet Red Bile Glucose Agar (VRBG Agar) Selective medium containing glucose for the detection and enumeration of Enterobacteriaceae from food and dairy products. Vitamin B12 Assay Medium Microbiological assay of Vitamin B12. Use for determining vitamin B12 concentration by the microbiological assay technique. VJ Agar VJ Agar, also known as Vogel and Johnson Agar, is used for the early detection of coagulasepositive, mannitol-fermenting staphylococci. Use with Chapman Tellurite Solution 1 % ( ). Use for isolating coagulase-positive mannitol-fermenting staphylococci from clinical or food specimens Wilkins Chalgren Agar Susceptibility testing of anaerobic bacteria by the agar diffusion method. WL Differential Medium WL Differential Medium = Wallerstein Laboratory Differential Medium. Green and Gray developed WL Differential Medium that inhibits the growth of yeasts without inhibiting the growth of bacteria present in beers. Used for isolating bacteria encountered in brewing and industrial fermentation processes. WL Nutrient Broth Wallerstein Laboratory Nutrient Broth. Cultivation of yeasts, molds and bacteria encountered in brewing and industrial fermentation processes. WL Nutrient Medium Wallerstein Laboratory Medium. Cultivation of yeasts, molds and bacteria encountered in brewing and industrial fermentation processes. Wort Agar For Cultivation and Enumeration of Yeasts. BD Diagnostics Diagnostic Systems Catalog 2008/

50 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Culture & Analysis Media: BD Bacto - BBL - Difco Quality kg kg kg g g g kg kg g kg XL Agar Base XL (Xylose Lysine) Agar Base is used for the isolation and differentiation of enteric pathogens and, when supplemented with appropriate additives, as a base for selective enteric media. It was developed by Taylor for the nonselective isolation and differentiation of gram-negative enteric bacilli. Dehydrated Appearance: Fine, homogeneous, free of extraneous material. XLD Agar Xylose Lysine Desoxycholate Agar. Selective differential medium for the isolation of gramnegative enteric bacilli, especially Shigella and Providencia. XLT4 Agar Base XLT4 Agar Base is used with XLT4 Agar Supplement ( ) in isolating non-typhi Salmonella. Contains peptone as a source of complex nitrogen compounds. Dehydrated Appearance: Pink, free flowing, homogeneous. Yeast Carbon Base Wickerham formula. Use for the classification of yeasts based on nitrogen assimilation. Yeast Extract Glucose Chloramphenicol Agar Selective agar recommended by the International Dairy Federation for the enumeration of yeasts and molds in milk and milk products. Yeast Morphology Agar Wickerham formula. Use for the classification of yeasts based on colonial characteristics and cell morphology. Yeast Nitrogen Base Wickerham formula. Use for the classification of yeasts based on carbon assimilation. Yeast Nitrogen Base without Amino Acids Wickerham formula. Use for the classification of yeasts based on amino acid and carbohydrate requirements. Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate Wickerham formula. Used for the classification of yeasts based on nitrogen and carbon requirements. Yersinia Selective Agar Base (CIN Agar Base) CIN (Cefsulodin-Irgasan-Novobiocin) Agar Base, when supplemented with cefsulodin and novobiocin (Yersinia Antimicrobic Supplement CN, ), is a differential and selective medium used in qualitative procedures for the isolation of Yersinia enterocolitica from a variety of clinical and nonclinical specimens. YM Agar Yeast Mold Agar is used for cultivating yeasts, molds and other aciduric microorganisms. Dehydrated Appearance: Beige, free-flowing, homogeneous. YM Broth Yeast Mold Broth is for Cultivation of yeasts, molds and other aciduric microorganisms BD Diagnostics Diagnostic Systems Catalog 2008/2009

51 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 10 ml x 10 ml x 10 ml g x 20 ml x 20 ml Antimicrobic Vial A Antimicrobic Vial A, containing chlortetracycline, is used in preparing selective microbiological culture media. Chlortetracycline selectively inhibits bacterial growth by inhibiting protein synthesis and restricts the size and height of colonies of more rapidly growing molds. Contains 25 mg desiccated chlortetracycline per 10 ml vial. The resulting concentration of the rehydrated solution is 2.5 mg chlortetracycline per ml. Can be used with: Cooke Rose Bengal Agar ( ). 6 x 10 ml Vials. Antimicrobic Vial K Antimicrobic Vial K, containing kanamycin, is used to supplement SFP Agar Base containing Egg Yolk Enrichment 50 % and Antimicrobic Vial P for the detection and enumeration of Clostridium perfringens in foods. Clostridia are not inhibited by kanamycin, which inhibits protein synthesis in susceptible organisms. 25 mg Kanamycin per 10 ml vial. Use with: SFP Agar Base ( ). 6 x 10 ml Vials. Antimicrobic Vial P Contains Polymyxin B. For enumerating Bacillus cereus from foods use with: MYP Agar ( ) and Egg Yolk Enrichment 50 % (Cat. Nos and ) Also for use with: SFP Agar Base ( ). 6 x 10 ml Vials. Bile Salts No. 3 Selective agent, inhibits gram-positive organisms. Dubos Medium Albumin A 5 % solution of albumin fraction V from bovine plasma and 7.5 % dextrose in normal saline. For the cultivation of M. tuberculosis use with: Dubos Broth Base ( ). 12 x 20 ml Prepared Tubes. Dubos Oleic Albumin Complex Dubos Oleic Albumin Complex and penicillin are used to supplement Dubos Oleic Agar Base for the isolation and susceptibility testing of Mycobacterium tuberculosis. Dubos and Middlebrook described Dubos Oleic Medium Albumin as suitable for primary isolation and cultivation of the tubercle bacillus and for studying colony morphology. In comparative studies, Dubos Oleic Albumin Agar Medium was superior to other media studied for primary isolation. Oleic acid is a carbon source. Albumin Fraction V is a growth factor. Dubos Oleic Albumin Complex is a 0.05 % solution of alkalinized oleic acid in a 5 % solution of albumin fraction V in normal saline (0.85 %). Use with: Dubos Oleic Agar Base ( ). 12 x 20 ml Prepared Tubes. BD Diagnostics Diagnostic Systems Catalog 2008/

52 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 10 ml x 100 ml ml x 100 ml x 100 ml ml x 5 ml x 10 ml ml x 2 ml x 10 ml x 5 ml Egg Yolk Enrichment (50 %) BD Bacto Egg Yolk Enrichment 50 % is a concentrated egg yolk emulsion recommended for use in a variety of media such as SFP Agar Base and McClung Toabe Agar Base for the isolation and identification of Clostridium species on the basis of their lecithinase activity. BD Bacto Egg Yolk Enrichment 50 %, which has been warmed to 45 50º C, is aseptically added to prepared and sterilized culture media which has been cooled to 45 50º C. In SFP Agar Base and Difco McClung Toabe Agar Base, 100 ml Egg Yolk Enrichment 50 % is added to 900 ml or 10 ml to 90 ml of prepared and sterilized base. Use with: McClung Toabe Agar Base ( ) MYP Agar ( ) SFP Agar Base ( ). 12 x 10 ml Prepared Tubes resp. 6 x 100 ml Bottles. Egg Yolk Enrichment (Gamma Irradiated) Used as a supplement in the preparation of various culture media for the detection of lecithinase and/or lipase, such as MYP Agar. Ready-to-use supplement: 100 ml Medium in 100 ml Plastic Bottle. Egg Yolk Tellurite Enrichment The enrichment consists of 30 % egg yolk suspension with 0.15 % potassium tellurite. For the isolation of S. aureus use with: Baird-Parker Agar Base (Cat. Nos and ). 6 x 100 ml Bottles. Egg Yolk Tellurite Enrichment (Gamma Irradiated) Used as a supplement in the preparation of culture media for the detection of tellurite reduction and lecithinase and/or lipase reaction, such as Baird Parker Agar. Ready-to-use supplement: 100 ml Medium in 100 ml Plastic Bottle. Fildes Enrichment May be used to enrich a variety of media for the cultivation of various microorganisms. 10 x 5 ml Tubes (K size). Fraser Broth Supplement Contains 0.05 g Ferric Ammonium Citrate. The medium is used in the rapid detection of Listeria from food and environmental samples. Use with: Fraser Broth Base ( and ) and Demi-Fraser Broth Base (Cat. Nos and ). 6 x 10 ml Vials. Iodine Solution Additive to be used with: Tetrathionat Broth Base ( and ). 40 ml Medium in 50 ml Screw Cap Bottle. BD IsoVitaleX Enrichment Chemically defined supplement used as an additive to media for the isolation and cultivation of nutritionally fastidious microorganisms. BD IsoVitaleX Enrichment with Rehydrating Fluid is used for supplementing media to culture fastidious microorganisms, particularly Neisseria gonorrhoeae and Haemophilus influenzae : 5 lyophilized vials; (each reconstitutes to 2 ml) and 5 vials diluent : 5 lyophilized vials; (each reconstitutes to 10 ml) and 5 vials diluent. Legionella Agar Enrichment L-cysteine and ferric pyrophosphate. For the isolation of Legionella. BD Bacto BD Bacto 2-34 BD Diagnostics Diagnostic Systems Catalog 2008/2009

53 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 10 ml x 100 ml x 20 ml kg kg x 2 ml x 20 ml x 100 ml Leptospira Enrichment (Lyophilized) For use in the enrichment of media for the cultivation of Leptospira species. Media such as Fletcher Medium Base and Stuart Broth Base are used with rabbit serum enrichment for the detection of leptospires in blood, spinal fluid, urine, waters and other minerals. They are useful therefore, in diagnostic and epidemiological studies of leptospirosis in humans and animals. Leptospira Enrichment provides the necessary enrichment for these media. Box of 10 vials, lyophilized, each vial restores to 10 ml. Leptospira Enrichment EMJH To cultivate and maintain Leptospira use with: Leptospira Medium Base EMJH ( ). 6 x 100 ml Bottle. Lipase Reagent For detecting and enumerating lipolytic microorganisms use with: Spirit Blue Agar ( ). 6 x 20 ml Bottles. Listeria Enrichment Broth Selective enrichment for L. monocytogenes from nondairy and processed food products. Listeria Enrichment Broth, Modified Used for selectively enriching Listeria from raw and pasteurized milk according to the International Dairy Federation (IDF). Listeria Selective Supplement For isolation and cultivation of Listeria monocytogenes use with: LPM Agar Base ( ). Pkg. of 10 vials, lyophilized; each reconstitutes to 2 ml. Middlebrook ADC Enrichment Used to supplement culture media for the cultivation of Mycobacteria (for Middlebrook 7H9 Broth, ) : Prepared Tubed Media: 10 x 20 ml per tube, "A" size tube : Prepared Bottled Media: 6 x 100 ml per bottle x 20 ml x 100 ml ml Middlebrook OADC Enrichment Used to supplement culture media for the isolation and cultivation of Mycobacteria for Middlebrook 7H10 Agar, Cat. Nos and and Mycobacteria 7H11 Agar, : Prepared Tubed Media: 10 x 20 ml per Tube, "A" size Tube : Prepared Bottled Media: 6 x 100 ml per Bottle : Prepared Bottled Media: 1 x 500 ml per Bottle. BD Diagnostics Diagnostic Systems Catalog 2008/

54 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 30 ml x 30 ml x 10 ml x 10 ml x 10 ml x 3 ml x 1 g Mycoplasma Enrichment without Penicillin Mycoplasma Enrichment without Penicillin is a sterile desiccated enrichment for use in PPLO media as described by Hayflick. The supplements are prepared according to the formulations of Chanock, Hayflick and Barile and Hayflick. 10 x 30 ml Vials for use with: PPLO Agar (Mycoplasma Agar) ( ) Mycoplasma Agar Base (PPLO Agar Base) ( ) PPLO Broth (Mycoplasma Broth) ( ) Mycoplasma Broth Base (PPLO Broth Base) ( ) Mycoplasma Broth Base (Frey) (Cat. Nos and ) Lyophilized - 10 x 30 ml Vials. Mycoplasma Supplement For the isolation and cultivation of Mycoplasma spp.; e.g., PPLO media, Heart Infusion Agar and Heart Infusion Broth. 6 x 30 ml Vials for use with: PPLO Agar (Mycoplasma Agar) ( ) Mycoplasma Agar Base (PPLO Agar Base) ( ) PPLO Broth (Mycoplasma Broth) ( ) Mycoplasma Broth Base (PPLO Broth Base) ( ) Mycoplasma Broth Base (Frey) (Cat. Nos and ) 6 x 30 ml Vials. Novobiocin Antimicrobic Supplement Contains 20 mg of Novobiocin per liter of final medium. May be used with: EC Medium - Modified ( ) or Rappaport-Vassiliadis (MRSV) Medium Semisolid Modification ( ) or Brilliant Green Agar ( ). 6 x 10 ml Vials. Oxford Antimicrobic Supplement, Modified With Moxalactam and Colistin Sulfate. Use with: Oxford Medium Base ( ). 6 x 10 ml Vials. PALCAM Antimicrobic Supplement For use with: PALCAM Medium Base ( ). 3 x 10 ml Vials. Rose Bengal Antimicrobic Supplement For selective isolation and enumeration of yeasts and molds from foods, dairy products and the environment use with: Rose Bengal Agar Base ( ) 10 x 3 ml Vials. Rosolic Acid Use with: m FC Agar (Cat. Nos and ) and m FC Broth Base (Cat. Nos and ). 6 x 1 g Vials. SBG Sulfa Enrichment Selenite Brilliant Green Sulfa Enrichment is a selective enrichment for the isolation of Salmonella. With 0.1 % Sulfapyridin. SBG Sulfa Enrichment is used for enriching Salmonella prior to isolation procedures. Use with: BG Sulfa Agar ( ) BD Diagnostics Diagnostic Systems Catalog 2008/2009

55 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 10 ml x 5 ml ml x 10 ml ml x 15 ml g ml Supplement B Supplement B with Reconstituting Fluid B is used for supplementing media to culture fastidious microorganisms, particularly Neisseria gonorrhoeae and Haemophilus influenzae. May be used with: Eugon Agar ( ) Proteose No. 3 Agar ( ). Lyophilized 6 10 ml with Reconstituting Fluid. Supplement C This is a desiccated yeast concentrate used to supplement media for cultivating fastidious organisms with exacting growth requirements. Supplement C contains the thermolabile and thermostable growth accessory factors of fresh yeast, Including glutamine, coenzyme (V factor), hematin (X factor), cocarboxylase and other growth factors required for the growth of fastidious organisms. Supplement VX Sterile concentrate of essential growth factors V and X. For cultivation of fastidious microorganisms like N. gonorrhoeae and H. influenzae. For use with: Proteose No. 3 Agar ( ) : Lyophilized 1 x 100 ml with Reconstituting Fluid : Lyophilized 6 10 ml with Reconstituting Fluid. Tellurite Solution (1 %) For use with: Mitis Salivarius Agar ( ) and Tellurite Glycine Agar ( ) and VJ Agar ( ) Prepared Vial 20 ml. Tinsdale Enrichment Tinsdale Enrichment Desiccated is used with Tinsdale Agar Base (Cat. No ) for primary isolation and differentiation of Corynebacterium diphtheriae. The Enrichment contains bovine serum and horse serum which provide essential growth factors. L- cystine and sodium thiosulfate provide sulfur for H2S production. Potassium tellurite is a selective agent. The formation of black to brown halos surrounding the colony results from the reduction of potassium tellurite by H2S to metallic tellurite. Stabbing the complete Tinsdale Agar with an inoculating needle accentuates darkening of the medium by C. diphtheriae. Use with: Tinsdale Agar Base ( ). 6 x 15 ml Tubes. TTC Solution (1 %, Sterile) TTC Solution 1% (Triphenyltetrazolium Chloride) is ready for use in the preparation of culture media. For use with KF Streptococcus Agar ( ) : Bottle 25 g : prepared Tube 30 ml. Universal Preenrichment Broth Used for recovering sublethally injured Salmonella and Listeria from food products. Traditional methods for recovering Salmonella and Listeria from food products require separate preenrichment media for each microorganism. Some broth media recommended for preenrichment contain antibiotic inhibitors or have insufficient buffering capacity which hinder recovery of sublethally injured cells. BD Diagnostics Diagnostic Systems Catalog 2008/

56 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Inhibitors, Enrichments & Supplements: - Quality x 10 ml x 10 ml x 2 ml x 10 ml x 10 ml x 10 ml ml x 10 ml V-C-A Inhibitor Antibiotic mixture of vancomycin, colistin and anisomycin which is incorporated into culture media to permit the isolation of pathogenic Neisseria by inhibiting contaminating flora. Lyophilized ml. VCAT Inhibitor Antibiotic mixture of vancomycin, colistin, anisomycin and trimethoprim. VCA inhibitor plus trimethoprim to improve the recovery of pathogenic Neisseria by increasing the selectivity of isolation media. Lyophilized ml. V-C-N Inhibitor Antibiotic mixture of vancomycin, colistin and nystatin that permits the selective isolation of Neisseria gonorrhoeae and N. meningitidis from culture media : 10 vials, lyophilized; each reconstitutes to 2 ml : 10 vials, lyophilized; each reconstitutes to 10 ml. V-C-N-T Inhibitor Antibiotic mixture of vancomycin, colistin, nystatin and trimethoprim that improves the recovery of pathogenic Neisseria by increasing the selectivity of isolation media. 10 vials, lyophilized; each reconstitutes to 10 ml. Vitamin K1-Hemin Solution Vitamin K1 - Hemin Solution is used as a culture medium enrichment for anaerobic microorganisms. 10 x 10 ml Tubes. XLT4 Supplement Added to inhibit growth of non-salmonella organisms. To be used with: XLT4 Agar Base ( and ). 1 x 100 ml Bottle. Yersinia Antimicrobic Supplement CN Yersinia Antimicrobic Supplement CN is used in the preparation of Yersinia Selective Agar (CIN Agar). The complete medium, based on the Cefsulodin-Irgasan-Novobiocin (CIN) Agar formulation of Schiemann, is recommended for use in the selective isolation and cultivation of Yersinia enterocolitica from clinical and nonclinical sources. Use with: Yersinia Selective Agar Base ( ). 6 x 10 ml Vials BD Diagnostics Diagnostic Systems Catalog 2008/2009

57 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Molecular Genetics Media: Quality kg kg kg kg kg 2 YT Medium (Yeast Extract Tryptone) Formulation designed for growth and propagation of E. coli infected with the single strand filamentous bacteriophage M13. LB Agar (Lennox) A nutritionally rich media developed by Lennox for the growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12, which are deficient in B vitamin production. This strain of E. coli has been further modified through specific mutation to create an auxotrophic strain that is not capable of growth on nutritionally deficient media. LB Agar, Lennox provides all the nutritional requirements of these organisms. LB Agar, Lennox contains half the sodium chloride level of the Miller formulation of LB Agar. This allows the researcher to select the optimal salt concentration for a specific strain. LB Agar (Miller) LB (Luria-Bertani) Agar, Miller is used for maintaining and propagating Escherichia coli in molecular microbiology procedures. LB Agar, Miller is based on LB Medium as described by Miller for the growth and maintenance of E. coli strains used in molecular microbiology procedures. These are nutritionally rich media designed for growth of pure cultures of recombinant strains. E. coli grows more rapidly because they provide the cells with amino acids, nucleotide precursors, vitamins and other metabolites that the microorganism would otherwise have to synthesize. BD Select APS - LB Broth Base LB Broth Base (BD Select APS - Alternative Protein Source). For the propagation and maintenance of E. coli for molecular biology. Non-animal origin formulation,. LB Broth (Lennox) LB Broth, Lennox contains ten times the sodium chloride level of Luria Broth Base, Miller and one half of that found in LB Broth, Miller. This allows the researcher to select the optimal salt concentration for a specific strain. If desired, the medium may be aseptically supplemented with glucose to prepare the complete medium described by Lennox. LB Broth (Miller) LB (Luria-Bertani) Broth, Miller is used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Luria Agar Base (Miller) Used for maintaining and propagating Escherichia coli in molecular microbiology procedures with or without added glucose. Nutritionally rich medium designed for growth of pure cultures of recombinant strains, based on the Luria agar and broth formulae described by Miller. Contains one-tenth and one-twentieth, respectively, the sodium chloride level of the LB Agar, Lennox and LB Agar, Miller formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose, if desired Luria Broth Base (Miller) Luria Broth Base, Miller is used for maintaining and propagating Escherichia coli in molecular microbiology procedures with or without added glucose. BD Diagnostics Diagnostic Systems Catalog 2008/

58 Dehydrated Culture Media (DCM) BD Culture Media & Ingredients Molecular Genetics Media: Quality kg kg kg M9 Minimal Salts (5 ) Used in preparing M9 Minimal Medium which is used for cultivating recombinant strains of Escherichia coli. M9 Minimal Salts, 5 is a 5 concentrate that is diluted to a 1 concentration and supplemented with an appropriate carbon and energy source, such as dextrose, to provide a minimal, chemically defined medium. The medium will support the growth of wild-type strains of E. coli. M9 Minimal Salts is useful for maintaining positive selection pressure on plasmids coding for the ability to produce essential substances such as amino acids or vitamins. M9 Minimal Medium is also used to maintain stocks of F -containing bacteria for use with M13. The medium can be supplemented with specific amino acids or other metabolites, allowing for selection of specific auxotrophs. NZCYM Broth For cultivation of molecular genetic strains of E. coli for replication of recombinant l bacteriophage. NZYM Broth Similar to NZCYM without Casamino Acids. BD Select APS - Super Broth Base BD Select APS Super Broth is a molecular genetic medium that will grow E. coli to a high cell density. There is no glucose in the formulation thus preventing acetate build-up in the fermentation of the organism. Its Physical Characteristics: a tan, free-flowing powder. SOB Medium For cultivation of molecular genetic strains of E. coli. Terrific Broth For cultivation of molecular genetic strains of E. coli. Formulation developed to increase plasmid yield. YPD Agar For maintaining and propagating yeasts in molecular microbiology procedures. Dehydrated Appearance: Beige, free-flowing, homogeneous. YPD Broth Propagation and maintenance of yeasts for use in molecular biology BD Diagnostics Diagnostic Systems Catalog 2008/2009

59 Dehydrated Culture Media (DCM) BD Fermentation s Meat Hydrolysates/Blends: BD Bacto - BBL - BiTek - Difco Quality g kg kg kg Beef Heart for Infusion 100 g of this Desiccated Powder is equivalent to of fresh beef heart. Used in preparing microbiological culture media. Brain Heart Infusion BHI is a general-purpose medium used in the cultivation of fastidious and non-fastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. It serves as a base for supplemented media containing 0.1 % agar, Fildes enrichment or 6.5 % sodium chloride. A supplemented pre-reduced formulation in tubes is especially recommended for the cultivation of anaerobes. Brain Heart Infusion, Porcine For the cultivation of fastidious microorganisms using porcine as an alternate peptone source. Gelatin Gelatin is a protein of uniform molecular constitution derived chiefly from the hydrolysis of collagen. Collagens are a class of albuminoids found abundantly in bones, skin, tendon, cartilage and similar tissues of animals. Gelatin is used in culture media to detect gelatin liquefaction by bacteria and as a nitrogen and amino acid source. BD Bacto BD Bacto BD Bacto BD Bacto kg kg kg Non-Animal Origin s: BD Bacto - BBL - BiTek - Difco Quality Alternate Protein Source (APS). BD Select APS - Super Broth Base BD Select APS Super Broth is a molecular genetic medium that will grow E. coli to a high cell density. There is no glucose in the formulation thus preventing acetate build-up in the fermentation of the organism. Its Physical Characteristics: a tan, free-flowing powder. BD Select APS - Tryptic Soy Broth Alternative Protein Source obtained from animal-free components. BD Select APS - Tryptic Soy Broth (Sterile) Alternative Protein Source obtained from animal-free components. Serile, Gamma-Irradiated. BD Diagnostics Diagnostic Systems Catalog 2008/

60 Dehydrated Culture Media (DCM) BD Media Additives Chemicals: - Quality g x 10 ml x 20 ml g g kg g g g Adonitol Carbohydrate. For use in microbiological culture media in the study of fermentation reactions of bacteria. Is usually employed at a concentration of 0.5 to 1.0 % in fermentation media. Asparagine Amino acid for chemical and microbiological usage. Bovine Albumin (Fraction V) 0.2 % in 0.85 % Saline. Supplied in liquid form for use in specimen digestion procedures for the isolation and detection of Mycobacterium species. Bovine albumin is also known as bovine serum albumin or BSA. Used to enrich media for cultivating a large variety of microorganisms and tissue cells. 10 x 10 ml Tube. Bovine Albumin (5 %) Used to enrich media for cultivation a large variety of microorganisms and tissue cells. It is also known as bovine serum albumin or BSA. Bovine albumin can be added to normally sterile specimens, tissues and body fluids for direct inocultaion onto culture media used for isolating mycobacteria. BSA is also used as an enrichment when contaminated specimens are digested. Prepared Tubes, 12 x 20 ml. Cellobiose Carbohydrate; Cellobiose (+), anhydrous, neither D nor L. D-Galactose Carbohydrate; D-Galactose (+), anhydrous. D-Mannitol D-Sorbitol Sorbite D-Xylose Use with XLT4 Agar Base ( ). Dextrose Glucose Glucose, D (+), anhydrous. For incorporation into microbiological culture media as a source of energy for bacteria and for the determination of fermentation reactions. It is usually employed at a concentration of 0.5 to 1.0 % in fermentation media. Dulcitol Galactitol Esculin A water-soluble glycoside for the preparation of culture media used for the identification of various organisms, including enterobacteriaceae, enterococci and anaerobes. The test is used to differentiate group D streptococci, e.g. a S. faecalis that hydrolyzes esculin, from non-group D streptococci, e.g. a S. agalactiae that does not hydrolyze esculin. Hydrolysis of esculin yields esculetin, which forms a brown-black complex in the presence of a ferric salt. Glycerol Highly purified alcohol used as a fixative in bacterial preservation media and in the isolation and cultivation of many organisms BD Diagnostics Diagnostic Systems Catalog 2008/2009

61 Dehydrated Culture Media (DCM) BD Media Additives Chemicals: - Quality x 100 ml g g kg g ml g g g g g ml Hemoglobin Solution (2 %) Ready for use in the preparation of media for the cultivation of fastidious organisms. 10 x 100 ml Bottles. Inositol Carbohydrate; Inosite, Mesoinositol, neither D nor L. L-Arabinose Carbohydrate; For use in microbiological culture media in the study of fermentation reactions of bacteria. It is usually employed at a concentration of 0.5 to 1.0 % in fermentation media. Maltose Maltose (+), monohydrate. For use in microbiological culture media in the study of fermentation reactions of bacteria. It is usually employed at a 0.5 to 1.0 % concentration in fermentation media. Melibiose Melibiose (+), monohydrate, neither D nor L. Oxgall Oxgall is dehydrated bile used for preparing microbiological culture media, especially for selective media used to differentiate groups of bile-tolerant bacteria. Oxgall is used as a selective agent for the isolation of gram-negative microorganisms, inhibiting gram-positive bacteria. The major components of Oxgall are taurocholic and glycocholic acids. Polysorbate 80 Surface-activating ingredient. Use in Fluorescent Treponemal Antibody (FTA-ABS) diluents, for incorporation into microbiological culture media and other reagents to enhance their productivity and reactivity. Serves as a neutralizer of preservatives, allowing microorganisms to replicate. 100 ml Bottle. Raffinose D-Raffinose, pentahydrate. Rhamnose Saccharose D-Saccharose, Sucrose. Salicin Sodium Desoxycholate Sodium Desoxycholate is the sodium salt of desoxycholic acid (a highly purified bile acid) and can be used in culture media in lower concentrations than in naturally occurring bile. As with other bile salts can be used as selective agents for the isolation of gram-negative microorganisms, inhibiting gram-positive organisms and spore forming bacteria. Tellurite Solution (1 %) For use with: Mitis Salivarius Agar ( ) and Tellurite Glycine Agar ( ) and VJ Agar ( ) Prepared Vial 20 ml. BD Diagnostics Diagnostic Systems Catalog 2008/

62 Dehydrated Culture Media (DCM) BD Media Additives Chemicals: - Quality g g Trehalose Tween 80 - Polysorbate 80 Polysorbate 80, U.S.P. It is used to prepare 2 % Tween 80, which acts as a dispersing agent BD Diagnostics Diagnostic Systems Catalog 2008/2009

63 Prepared Media Since 1935, prepared media products have brought to the microbiology laboratory the highest levels of quality and performance. BD BBL TM Prepared Plated Media 3-2 BD BBL TM Prepared Tubed Media 3-18 BD Prepared Bottled Media 3-28 BD Prepared Slide Media for Urine Diagnostic 3-32 BD Prepared Media for Environmental Monitoring 3-34

64 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Aeromonas Yersinia Agar Aeromonas Yersinia Agar is a selective differential medium for the isolation of both Yersinia enterocolitica and Aeromonas spp. from a variety of clinical and nonclinical specimens. Aeromonas Yersinia Agar is a modification of CIN Agar that supports growth of Aeromonas species due to a reduced cefsulodin concentration and also supports growth of Yersinia enterocolitica. Bacteroides Bile Esculin Agar with Amikacin (BBE Agar) Selective medium for the isolation and presumptive identification of the Bacteroides fragilis group. Baird-Parker Agar Baird-Parker Agar is a moderately selective and differential medium for the isolation and enumeration of Staphylococcus aureus in foods, environmental, and clinical specimens. BCYE Agar Buffered Coal Yeast Agar is used for the isolation and cultivation of Legionella species. BCYE Agar with Vancomycin and Colistin Selective medium for the isolation of Legionella species from water and clinical specimens. BCYE Agar without Cysteine with Pimaricin BCYE Agar without Cysteine with Pimaricin (= BCYE Cys ) is a control medium to detect the presence of bacteria other than Legionella which may also appear on the other two media (Cat. No Legionella Agar (NEN) and Legionella Agar (NEN) without Antibiotics with Pimaricin). Legionella BCYE Agar without Cysteine with Pimaricin contains all the ingredients except cysteine, one of the two factors needed for recovery of Legionella. This medium does not allow growth of Legionella. Bifidobacterium Agar, Modified (Beerens) This Agar is a partially selective medium for the isolation of bifidobacteria from human stool specimens. BiGGY Agar Bismuth Sulfite Glucose Glycine Yeast Agar is a partially selective and differential medium for the isolation of Candida species form clinical specimens. Bordet Gengou Agar with 15 % Sheep Blood Bordet Gengou Agar with 15 % Sheep Blood is a selective medium for the isolation of Bordetella pertussis and B. parapertussis. Bordetella Agar with Charcoal and 7 % Horse Blood Bordetella Agar with Charcoal and 7 % Horse Blood is a selective medium for the isolation of Bordetella pertussis and B. parapertussis. Brain Heart Infusion Agar Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. BHI Agar is one formulation in which meat infusion is used, although, unlike in the earlier days, the infusion components are solids resulting from the drying of the liquid infusion material rather than the liquid components themselves. Peptones are also included as sources of nutrients. BHI Agar has proven to be effective in the cultivation of a wide variety of microorganisms, including many types of pathogens. BHI Agar can be used as a general medium for aerobic bacteriology and for the primary recovery of fungi from clinical specimens. 3-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

65 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Brain Heart Infusion Agar with 5 % Horse Blood and Bacitracin BHI Agar with 5 % Horse Blood and Bacitracin is a selective medium for the isolation of Haemophilus species, especially H. influenzae from clinical specimens. Used in the isolation of Haemophilus influenzae from clinical specimens and the screening of the normal oral flora of individuals for the presence of H. influenzae. Brain Heart Infusion Agar with 10 % Sheep Blood (Deep Fill) BHI Agar with 10 % Sheep Blood is used for the isolation and cultivation of fastidious and other organisms, especially those requiring blood for growth. It may also be used for the cultivation of pathogenic fungi and aerobic Actinomycetales. Brilliant Green Agar, Modified Brilliant Green Agar Modified is used for isolating Salmonella from water, sewage and foodstuffs. It may also be used for clinical specimens. Brilliant Green Agar Modified is more selective than Desoxycholate Citrate Agar and other brilliant green media, and inhibits the growth of Pseudomonas aeruginosa and partially inhibits the growth of Proteus spp. which may resemble Salmonella. Brilliant Green High Selectivity Agar Brilliant Green Agar is a highly selective medium for the isolation of Salmonella other than S. typhi from feces and other materials. Brilliant Green Agar was first described by Kristensen et al. in Their formulation was modified slightly by Kauffmann in The medium is included in procedures for the examination of water and wastewater. The medium is also included in the USP for use in the performance of Microbial Limit Tests. It is classified as a highly selective medium for the recovery of salmonellae except for the typhoid and paratyphoid bacilli. Bromcresol Purple Lactose Agar Bromcresol Purple Lactose Agar is a differential, non-selective medium for the isolation and enumeration of Enterobacteriaceae from clinical specimens, mainly from urine. As the medium is not inhibitory to many bacterial groups, also non-enterobacteriaceae will grow on this medium. The medium allows the differentiation of the bacteria recovered into lactose fermenters and nonfermenters. Brucella Agar with 5 % Horse Blood Brucella Agar with 5 % Horse Blood is a non-selective Medium which is used for the isolation and growth of both fastidious and nonfastidious bacterial species, including Brucella spp., Haemophilus spp, Strptococcus pneumoniae. Brucella Agar with 5 % Sheep Blood, Hemin and Vitamin K1 Brucella Agar with 5 % Sheep Blood, Hemin and Vitamin K1 is used for the isolation and cultivation of fastidious, obligately anaerobic microorganisms. The isolation of obligately anaerobic bacteria from clinical and nonclinical materials requires the use of selective, nonselective and enrichment media. Brucella Agar with 5 % Sheep Blood, Hemin and Vitamin K1 is an enriched, nonselective medium for the isolation and cultivation of a wide variety of obligately anaerobic microorganisms. Campylobacter Agar (Butzler) with 7 % Horse Blood Campylobacter Agar (Butzler) with 7 % Horse Blood is a selective medium for the isolation of Campylobacter species from clinical and other specimens. Campylobacter Agar Skirrow Campylobacter Agar (Skirrow) is a selective medium for the isolation of Campylobacter species from clinical and other specimens. BD Diagnostics Diagnostic Systems Catalog 2008/

66 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Campylobacter Agar with 5 Antimicrobics and 10 % Sheep Blood (CampyBAP) Campylobacter Agar with 5 Antimicrobics and 10 % Sheep Blood (Campy-BAP) is a selective medium for the primary isolation of Campylobacter jejuni and other cephalothin-resistant Campylobacter species from stool specimens. Campylobacter Selective Medium (Preston, Bloodfree) Campylobacter Selective Medium, Bloodfree is a selective medium for the isolation of Campylobacter species from intestinal and other specimens. CDC Anaerobe 5 % Sheep Blood Agar CDC Anaerobe 5 % Sheep Blood Agar is used for the isolation and cultivation of fastidious and slow growing, obligate anaerobic bacteria from a variety of clinical and nonclinical materials. It also supports good growth of most aerobic, facultatively anaerobic and microaerophilic bacteria if incubated appropriately. CDC Anaerobe 5 % Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe 5 % Sheep Blood Agar with Phenylethyl Alcohol (PEA) is used for the selective isolation of fastidious and slow-growing, obligately anaerobic bacteria from a variety of clinical and nonclinical materials. CDC Anaerobe Agar with Laked Sheep Blood, Kanamycin and Vancomycin Used for the selective isolation of fastidous, slow growing, anaerobic bacteria from a variety of different clinical and nonclinical materials. Cepacia Medium Cepacia Medium is a plated selective differential medium for the isolation of Burkholderia cepacia from clinical specimens (in particular mucoviscidosis patients). Chocolate Agar (Blood Agar No. 2 Base) Chocolate Agar (Blood Agar No. 2 Base) is a medium for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species. It is an alternative medium for the isolation of fastidious organisms from clinical specimens. Chocolate Agar (GC Agar with BD IsoVitaleX ) Chocolate Agar (GC Agar with BD IsoVitaleX ) is an improved medium for use in qualitative procedures for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from a variety of clinical specimens within 24 hours. Chocolate Agar with BD IsoVitaleX and Bacitracin Chocolate Agar with BD IsoVitaleX and Bacitracin and Brain Heart Infusion Agar with 5 % Horse Blood and Bacitracin are selective media for the isolation of Haemophilus species, especially H. influenzae from clinical specimens. CLED Agar CLED Agar (Cystine-Lactose-Electrolyte-Deficient Agar) is a differential culture medium for use in enumerating bacteria in urine. It supports the growth of urinary pathogens and contaminants but prevents undue swarming of Proteus species due to its lack of electrolytes. CLED Agar (Bevis) CLED Agar (Bevis) is a modified CLED Agar used for the isolation and determination of the viable count from urine specimens. Clostridium Difficile Agar with 7 % Sheep Blood This is a selective medium for the primary isolation of Clostridium difficile from fecal specimens. 3-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

67 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Clostridium Difficile Selective Agar CDSA Agar is recommended as a aslective and differential medium for the primary isolation of Clostridum difficile from fecal specimens. Columbia Agar with 5 % Horse Blood This agar is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from clinical and nonclinical materials. On this medium, colonies tend to be larger and growth is more luxuriant than on media containing on other blood agar bases. Columbia Blood Agar is recommended as a primary isolation medium in the MiQ standards. In many European countries, this medium has become the most frequently used primary isolation medium for clinical specimens. Columbia Agar with 5 % Sheep Blood This is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. It derives its superior growth-supporting properties from the combination of two peptones, and yeast extract as a supplier of the B complex vitamins. On this medium, colonies tend to be larger and growth is more luxuriant than on media containing other blood agar bases. Columbia Blood Agar is recommended as a primary isolation medium in the MiQ standards and in other diagnostic manuals. In many European countries, this medium has become the most frequently used primary isolation medium for clinical specimens Columbia CNA Agar, Modified, with 5 % Sheep Blood Selective and differential media used for the isolation and differentiation of gram-positive microorganisms from clinical and nonclinical materials. Columbia CNA Agar, Modified, with Crystal Violet and 5 % Sheep Blood Modified CNA Agar with Crystal Violet and 5 % Sheep Blood is a selective medium for the isolation of Gram positive bacteria from clinical specimens, especially streptococci and enterococci. Columbia CNA Agar with 5 % Sheep Blood This is a selective and differential medium used for the isolation of gram-positive microorganisms from clinical and nonclinical materials. Columbia CNA Plus Agar with 5 % Sheep Blood BD Columbia CNA Agar with 5% Sheep Blood, Improved II is a selective medium used for the isolation of gram-positive micro-organisms, especially staphylococci and streptococci, from clinical specimens. Columbia III Agar with 5 % Sheep Blood This is a highly nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical material. D/E Neutralizing Agar D/E Neutralizing Agar media are used for neutralizing antiseptics and disinfectants and for the detection of microorganisms that survive treatment of surfaces and materials with such antiseptics. DCLS Agar Desoxycholate Citrate Lactose Sucrose Agar is a moderately selective differential medium for isolation of Salmonella, Shigella, and cholera vibrios from clinical and other specimens. BD Diagnostics Diagnostic Systems Catalog 2008/

68 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Dermatophyte Test Medium, Modified with Chloramphenicol This is a selective medium for the isolation of pathogenic fungi from cutaneous sources such as skin, hair, and nails. Because of the unavailability of one of the inhibitory agents, chlortetracycline, Dermatophyte Test Medium (DTM), Modified with Chloramphenicol is recommended as a substitute for the original DTM formation. Desoxycholate Agar Desoxycholate Agar is a slightly selective and differential plating medium used for isolating and differentiating gramnegative enteric bacilli. DNase Test Agar Differential medium used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens. Drigalski Lactose Agar This is a selective and differential medium for the detection of Enterobacteriaceae and certain Nonfermenters from clinical specimens. Drigalski Lactose Agar with CAZ (Ceftazidime) This Agar is used for detection of Enterobacteriaceae that produce extended spectrum betalactamases (ESBL) in clinical specimens. EMB Agar, Modified (Holt-Harris and Teague) Eosin Methylene Blue (EMB) Agar, Modified, Holt-Harris and Teague is a slightly selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric bacilli (Enterobacteriaceae and several other gram-negative rods) from both clinical and nonclinical specimens. Endo Agar Endo Agar is a slightly selective and differential medium for the isolation, cultivation and differentiation of gram-negative microorganisms from both clinical and non-clinical specimens. BD Enterococcosel Agar BD Enterococcosel Agar is a selective medium for the detection and enumeration of fecal enterococci from clinical and nonclinical specimens. In addition, staphylococci, other enterococci and Listeria monocytogenes grow on this medium. BD Enterococcosel Agar with Vancomycin (8μg/ml) Used for primary screening of asymptomatic gastrointestinal carriage of vancomycin-resistant enterococci (VRE). Enterococcus Screen Agar Enterococcus Screen Agar QUAD Plate with Streptomycin/Gentamicin/Vancomycin. Gardnerella Selective Agar with 5 % Human Blood Gardnerella Selective Agar with 5 % Human Blood is a partially selective and differential medium for the isolation of Gardnerella vaginalis from clinical specimens. A new formulation results in better stability of the medium (= no autohemolysis) and slightly stronger beta hemolysis of Gardnerella. BD GC-Lect Agar BD GC-Lect Agar is a selective plated medium providing enhanced growth and recovery of Neisseria gonorrhoeae and better inhibition of contaminating bacteria and fungi, including Capnocytophaga species in oropharyngeal specimens. 3-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

69 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Group A Selective Strep Agar with 5 % Sheep Blood (BD ssa ) A selective medium for use in the isolation and presumptive identification of group A streptococci from throat cultures and other specimens. Group B Streptococcus Differential Agar This Agar is used for the isolation and identification of Streptococcus agalactiae (Group B Streptococcus) from clinical specimens. GVPC Medium GVPC Medium (Glycine-Vancomycin-Polymyxin-Cycloheximide Medium) is a selective medium for the isolation of Legionella species from water and clinical specimens. BD GVPC Medium, as described by Dennis et al., contains glycine, vancomycin, polymyxin B, and cycloheximide. This supplement has been found to be superior to all other inhibitory supplements described for Legionella, especially in water testing. Due to the addition of cycloheximide, it is also inhibitory to fungi which occur frequently in water. GVPC Medium is recommended by BSI and ISO for the detection and counting of Legionella in water. Haemophilus Test Medium Agar HTM Agar is used in the antimicrobial disc diffusion susceptibility procedure for Haemophilus influenzae and related species as described in the Approved Standard M2-A7, published by the National Committee for Clinical Laboratory Standards (CLSI, formerly NCCLS). Heart Infusion Agar with 5 % Sheep Blood Heart Infusion Agar (= HIA) with 5 % Sheep Blood is a non-selective general purpose medium used for the isolation of nutritionally fastidious microorganisms. Hektoen Enteric Agar Hektoen Enteric Agar is a moderately selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric microorganisms from both clinical and nonclinical specimens. It is of particular importance as a medium for the isolation of Shigella and Salmonella species. Helicobacter Agar Helicobacter Agar is a selective medium for the isolation of Helicobacter pylori from gastric specimens. Iso Res Agar A semi-defined medium for the susceptibility testing of non-fastidious organisms with the agar diffusion (Kirby-Bauer) method. Kimmig Fungal Agar Used for the isolation, cultivation, and maintenance of fungi from clinical and other sources. LB Agar (Lennox) LB (Luria-Bertani) Agar (Lennox) is used for maintaining and cultivating recombinant strains of Escherichia coli. LBS Agar LBS Agar is a semi-defined, partially selective medium for the isolation and enumeration of lactobacilli from clinical and other specimens. A new formulatipn results in larger colonies and better recovery of Lactobacillus species as well as unsurpassed good inhibition of Enterobacteriaceae and several other accompanying organisms. Selectivity is not achieved by antimicrobials (therefore drug-resistant strains from the accompanying flora have no chance to grow). BD Diagnostics Diagnostic Systems Catalog 2008/

70 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Legionella Agar (NEN) with Antibiotics A medium for the detection and presumptive identification of Legionella species from water. Legionella Agar (NEN) without Antibiotics with Pimaricin A medium for the detection and presumptive identification of Legionella species from water. Listeria Agar Listeria Agar is a selective differential medium used for the isolation of Listeria species from clinical, food, and dairy samples. MacConkey Agar without Crystal Violet This is a differential, partially selective medium for the isolation of Enterobacteriaceae, staphylococci, and enterococci from specimens of sanitary importance and from clinical specimens such as urines. MacConkey Agar without Salt This is a differential, partially selective medium for the isolation of Enterobacteriaceae, staphylococci, and enterococci from specimens of sanitary importance and from clinical specimens such as urines. Improved inhibition of swarming Proteus spp. is obtained, making it suitable for urine analysis. MacConkey II Agar MacConkey II Agar is a slightly selective and differential medium for the detection of coliform organisms and enteric pathogens. MacConkey II Agar is a selective and differential medium. It is only slightly selective since the concentration of bile salts, which inhibit gram-positive microorganisms, is low in comparison with other enteric plating media. Crystal violet also is included in the medium to inhibit the growth of gram-positive bacteria, especially enterococci and staphylococci. MacConkey II Agar with MUG This Agar is used for the presumptive identification of Escherichia coli. MacConkey II Agar with Sorbitol This is a partially selective medium for the isolation of E. coli O157:H7 from clinical, veterinary, food, and environmental sources. Mannitol Salt Agar Mannitol Salt Agar is a selective and differential medium for the detection and enumeration of staphylococci from clinical and nonclinical specimens and their differentiation according to mannitol fermentation. Mannitol Salt Agar with Oxacillin Mannitol Salt Agar with Oxacillin is used as a screening medium for the detection and isolation of methicillin resistant Staphylococcus aureus (MRSA) in clinical specimens. Martin-Lewis Agar, Modified Martin-Lewis Agar, Modified is an enriched medium for the selective isolation of Neisseria gonorrhoeae and N. meningitidis from clinical specimens containing mixed flora of bacteria and fungi. 3-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

71 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Middlebrook and Cohn 7H10 Agar Middlebrook 7H10 Agar contains a variety of inorganic salts which provide substances essential for the growth of mycobacteria. The sodium citrate, when converted to citric acid, holds certain inorganic cations in solution. Glycerol is an abundant source of carbon and energy. Oleic acid, as well as other long chain fatty acids, can be utilized by tubercle bacilli and plays an important role in the metabolism of mycobacteria. Catalase destroys toxic peroxides that may be present in the medium. The primary effect of albumin is that of protection of the tubercle bacilli against toxic agents, therefore enhancing their recovery by primary isolation. Partial inhibition of bacteria is achieved by the presence of the malachite green dye. BD Mueller Hinton Agar with 5 % Sheep Blood Mueller Hinton Agar with 5 % Sheep Blood, available is several plate formats and package sizes, is recommended for disc diffusion susceptibility testing of Streptococcus pneumoniae and other streptococci as standardized by CLSI (formerly the National Committee for Clinical Laboratory Standards NCCLS). BD Mueller Hinton Chocolate Agar with BD IsoVitaleX Chocolate Agar with BD IsoVitaleX (Mueller Hinton Agar Base) may be used for the susceptibility testing of fastidious bacteria such as Neisseria gonorrhoeae and for the isolation and cultivation of fastidious bacteria from clinical specimens. BD Mueller Hinton II Agar Mueller Hinton II Agar, available in several plate formats and package sizes, is used in the standardized disc diffusion procedure for determining the susceptibility of rapidly-growing aerobic organisms to antimicrobial agents. Mycoplate MS Agar Mycoplate MS Agar is a non-selective medium for the cultivation of fungi, the differentiation of yeasts of the genus Candida using the germ tube test. It may also be used for the presumptive susceptibility testing of yeasts against 5-Flucytosin (= 5-FC) using the agar diffusion technique. It is not recommended as an isolation medium for fungi. BD Mycosel Agar BD Mycosel Agar is a highly selective medium containing cycloheximide and chloramphenicol. It is recommended for the isolation of pathogenic fungi from materials having a large flora of other fungi and bacteria. It is not a general purpose medium for the isolation of all fungi (including molds and saprophytic yeasts). MYP Agar Mannitol Egg Yolk Polymyxin Agar is a selective differential medium for the detection and enumeration of Bacillus cereus from foods. Neomycin Blood Agar Neomycin Blood Agar is used for the isolation of group A streptococci (S. pyogenes) and group B streptococci (S. agalactiae) from clinical specimens in which the presence of these organisms is suspected. Nutrient Agar Used for the cultivation and isolation of a wide variety of non-fastidious and moderately fastidious organisms. OFPBL Agar Used in the selective isolation and detection of Burkholderia (formerly Pseudomonas) cepacia from clinical and nonclinical specimens. BD Diagnostics Diagnostic Systems Catalog 2008/

72 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Oxacillin Screen Agar Oxacillin Screen Agar (originally named MRSA Screen Agar) was developed for the detection of methicillin-resistant Staphylococcus aureus (MRSA). These strains are resistant to penicillinaseresistant penicillins (PRPs), such as methicillin, oxacillin and nafcillin. Since the method to detect MRSA uses the same inoculum as the Bauer-Kirby antimicrobial disc susceptibility test procedure, the oxacillin screen test may be conveniently performed on isolates at the same time as routine susceptibility testing. PALCAM Listeria Agar PALCAM Agar is a selective differential medium for the isolation and detection of Listeria monocytogenes and other Listeria species from foods and clinical specimens. PALCAM Medium Base is used with PALCAM Antimicrobic Supplement ( ) in isolating and cultivating Listeria, particularly from foods and milk products. Plate Count Agar Plate Count Agar - also known as Standard Methods Agar - is a Standard Methods medium used for enumerating aerobic bacteria in water, wastewater, foods and dairy products. This medium is also recommended as a general plating medium for determining aerobic bacterial populations. Standard Methods Agar With Glucose is a modification which contains more glucose for improved growth of saccharolytic organisms. Potato Glucose Agar Used for the isolation and cultivation of yeasts and molds from foods and dairy products, other foods, cosmetics, and from environmental materials. Pseudomonas Agar P Pseudomonas Agar P, also known as Tech Agar, is used for the enhancement of pyocyanin production by Pseudomonas. Pseudomonas Agar P (Tech Agar) contains enzymatic digest of gelatin to provide amino acids and other essential nitrogenous substances. The gelatin peptone is low in phosphorous to minimize the inhibitory action on pyocyanin production. Magnesium, potassium and sulfate ions promote pyocyanin production. Contains glycerol, which acts as a source of energy and enhances pigment production. Pseudomonas CFC Agar A ready-to-use medium for the isolation of Pseudomonas species and related organisms (e.g. Burkholderia cepacia) from food, water, pharmaceutical materials and environmental samples. Pseudomonas Isolation Agar Pseudomonas Isolation Agar is used with added glycerol in isolating Pseudomonas and differentiating Pseudomonas aeruginosa from other pseudomonads based on pigment formation. As well as being selective, Pseudomonas Isolation Agar is formulated to enhance the formation of the blue or blue-green pyocyanin pigment by Pseudomonas aeruginosa. The pigment diffuses into the medium surrounding growth. BD Pseudosel Agar BD Pseudosel Agar is used for the selective isolation of Pseudomonas aeruginosa from a variety of specimens. Allows detection of the pyocyanin generation. R2A Agar A ready-to-use medium for the performance of heterotrophic plate counts and for subcultures of bacteria isolated from potable water samples. It is recommended for use in standard methods for pour plate, spread plate and membrane filter analyses : plate of 60 mm diameter BD Diagnostics Diagnostic Systems Catalog 2008/2009

73 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Rice Extract Agar Rice Extract Agar is used for promotion of chlamydospore formation by Candida albicans and C. stellatoidea as a means of differentiating them from other Candida species. Sabouraud Agar with Chloramphenicol Used for the isolation of fungi that exhibit inhibition of bacteria. It is a selective medium for the isolation of pathogenic fungi that exhibit inhibition of bacteria and saprophytic fungi. Sabouraud Agar with Chloramphenicol and Cycloheximid A selective medium for the isolation of pathogenic fungi that exhibit inhibition of bacteria and saprophytic fungi. Sabouraud Agar with Gentamycin and Chloramphenicol Used for the isolation of fungi that exhibit inhibition of bacteria. It is a selective medium for the isolation of pathogenic fungi that exhibit inhibition of bacteria and saprophytic fungi. Sabouraud Agar with Penicillin and Streptomycin A selective media for the isolation of fungi that exhibit inhibition of bacteria. Sabouraud Dextrose Agar Sabouraud Dextrose Agar is used in qualitative procedures for cultivation of pathogenic and nonpathogenic fungi, particularly dermatophytes. The medium is rendered more selective for fungi by the addition of antimicrobics. Salmonella Shigella Agar Salmonella Shigella Agar (SS Agar) is a differential selective medium for the isolation of pathogenic enteric bacilli, especially those belonging to the genus Salmonella. This medium is not recommended for the primary isolation of Shigella. Schaedler Agar with Vitamin K1 and 5 % Sheep Blood Schaedler Agar with Vitamin K1 and 5 % Sheep Blood is a highly nutritious medium for the isolation and cultivation of fastidious anaerobic microorganisms. It is especially useful for the recovery of the fastidious anaerobic bacteria such as Bacteroides, Prevotella and Porphyromonas species. Schaedler CNA Agar with 5 % Sheep Blood A partially selective medium for the Isolation of strictly anaerobic Gram positive cocci and other anaerobic Gram positive bacteria from clinical specimens. Schaedler K-V Agar with 5 % Sheep Blood Schaedler Kanamycin-Vancomycin Agar with 5 % Sheep Blood is a highly nutritious, selective medium for the isolation and cultivation of fastidious gram-negative anaerobic microorganisms, especially Bacteroides and Prevotella species and a variety of other gram-negative anaerobes. Serum Tellurite Agar Serum Tellurite Agar is a selective and differential medium used for isolation of members of the genus Corynebacterium, particularly in the laboratory diagnosis of diphtheria. Seven H11 Agar (Deep Fill) Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Prepared Plates, Deep Fill. BD Diagnostics Diagnostic Systems Catalog 2008/

74 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) TCBS Agar Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS) is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens. Tellurite Agar (Hoyle) with 7 % Lysed Horse Blood A partially selective and differential medium for the isolation of Corynebacterium diphtheriae from clinical specimens and for determination of C. diphtheriae biovars. Triple Sugar Iron Agar Triple Sugar Iron Agar, when used as a plated medium, is a differential, non-selective medium for the differentiation of Enterobacteriaceae, especially the detection of hydrogen sulfide producing Salmonella. BD Trypticase Soy Agar General purpose media which support the growth of nonfastidious as well as moderately fastidious microorganisms. Not the medium of choice for fastidious anaerobes. BD Trypticase Soy Agar with 5 % Horse Blood (TSA II) BD Trypticase Soy Agar with 5 % Horse Blood (TSA II) is a nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials and the detection of hemolytic reactions. BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) is a nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials and the detection of hemolytic reactions. A new formulation results in much clearer beta hemolysis of beta hemolytic streptococci, improved beta hemolysis of Staphylococcus aureus and other beta hemolytic staphylococci, and enhaced growth of other bacteria. Vancomycin Screen Agar Containing Brain Heart Infusion Agar (BHIA) as its basal medium, the Vancomycin Screen Agar is suitable for microorganism cultivation and susceptibility screening as recommended by CLSI, formerly NCCLS. Vancomycin Screen Agar supports dependable testing for enterococcal isolates exhibiting vancomycin resistance. The yellow medium of Vancomycin Screen Agar helps identify the antimicrobial being tested. Violet Red Bile Glucose Agar (VRBG Agar) VRBG Agar is used for the enumeration and isolation of Enterobacteriaceae from foods and dairy products. Wilkins Chalgren Agar Wilkins Chalgren media are used for the isolation and cultivation of strictly anaerobic bacteria and may be used for the susceptibility testing of strict anaerobes. Wilkins Chalgren Agar with 5 % Horse Blood Wilkins Chalgren media are used for the isolation and cultivation of strictly anaerobic bacteria and may be used for the susceptibility testing of strict anaerobes. The addition of horse blood in Wilkins Chalgren Agar with 5 % Horse Blood supplies additional nutrients sufficient for the growth of fastidious anaerobes such as Porphyromonas and Peptostreptococcus BD Diagnostics Diagnostic Systems Catalog 2008/2009

75 Prepared Media Prepared Plated Media (PPM) PPMs (90 mm BD Stacker Plates) Wilkins Chalgren Agar with Amikacin and 7 % Sheep Blood Wilkins Chalgren Agar with Amikacin and 7 % Sheep Blood is a selective medium for the isolation of strictly anaerobic bacteria from clinical specimens. Due to the amikacin, most facultative organisms will be inhibited. XLD Agar Xylose Lysine Desoxycholate Agar is a moderately selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric pathogens from both clinical and non-clinical specimens (Salmonella and Shigella). It is especially suitable for the isolation of Shigella species. Yersinia Selective Agar (CIN Agar) Yersinia Selective Agar (= CIN = Cefsulodin-Irgasan-Novobiocin Agar) is a selective differential medium for the isolation of Yersinia enterocolitica and Aeromonas Yersinia Agar is a selective differential medium for the isolation of both Yersinia enterocolitica and Aeromonas spp. from a variety of clinical and nonclinical specimens. PPMs (120 mm Square Plates) Haemophilus Identification Plate (Hemo ID Plate) Contains one quadrant with X factor enriched medium, one with V factor-enriched medium (Quadrant II), one with both X and V factors-enriched medium, and one with and horse blood to determine growth and hemolysis. BD Mueller Hinton Agar with 5 % Sheep Blood Mueller Hinton Agar with 5 % Sheep Blood, available is several plate formats and package sizes, is recommended for disc diffusion susceptibility testing of Streptococcus pneumoniae and other streptococci as standardized by CLSI (formerly the National Committee for Clinical Laboratory Standards NCCLS). BD Mueller Hinton II Agar Mueller Hinton II Agar, available in several plate formats and package sizes, is used in the standardized disc diffusion procedure for determining the susceptibility of rapidly-growing aerobic organisms to antimicrobial agents. BD Diagnostics Diagnostic Systems Catalog 2008/

76 Prepared Media Prepared Plated Media (PPM) PPMs (150 mm Plates) GC II Agar with BD IsoVitaleX Enrichment GC II Agar with BD IsoVitaleX Enrichment is used for antimicrobial disc diffusion susceptibility testing of Neisseria gonorrhoeae. Haemophilus Test Medium Agar HTM Agar is used in the antimicrobial disc diffusion susceptibility procedure for Haemophilus influenzae and related species as described in the Approved Standard M2-A7, published by the National Committee for Clinical Laboratory Standards (CLSI, formerly NCCLS). Mueller Hinton II Agar Mueller Hinton II Agar, available in several plate formats and package sizes, is used in the standardized disc diffusion procedure for determining the susceptibility of rapidly-growing aerobic organisms to antimicrobial agents. Mueller Hinton Agar with 5 % Sheep Blood Mueller Hinton Agar with 5 % Sheep Blood, available is several plate formats and package sizes, is recommended for disc diffusion susceptibility testing of Streptococcus pneumoniae and other streptococci as standardized by CLSI (formerly the National Committee for Clinical Laboratory Standards NCCLS). PALCAM Listeria Agar PALCAM Agar is a selective differential medium for the isolation and detection of Listeria monocytogenes and other Listeria species from foods and clinical specimens. PALCAM Medium Base is used with PALCAM Antimicrobic Supplement ( ) in isolating and cultivating Listeria, particularly from foods and milk products. BD Trypticase Soy Agar General purpose media which support the growth of nonfastidious as well as moderately fastidious microorganisms. Not the medium of choice for fastidious anaerobes BD Diagnostics Diagnostic Systems Catalog 2008/2009

77 Prepared Media Prepared Plated Media (PPM) PPMs (Bi-Plates) BD CHROMagar Orientation // Columbia CNA Agar BD CHROMagar Orientation combined with Columbia CNA Agar is used for the isolation and pre-identification of bacteria commonly involved in urinary tract infections. While BD CHROMagar Orientation is a non-selective medium for the isolation, differentiation and enumeration of urinary tract pathogens, Columbia CNA Agar provides a further differentiation of Gram positive bacteria. BD CHROMagar Salmonella // XLD Agar BBL CHROMagar Salmonella is a selective differential medium for the isolation and presumptive identification of Salmonella, and XLD Agar (Xylose Lysine Desoxycholate Agar) is a moderately selective and differential medium for the isolation of Salmonella and Shigella. The combination of both media in a biplate allows simultaneous detection of Shigella and Salmonella. Columbia CNA Agar with 5 % Sheep Blood // MacConkey II Agar Columbia CNA Agar with 5 % Sheep Blood is a selective and differential medium used for the isolation of gram-positive microorganisms from clinical and nonclinical materials. MacConkey II Agar is a selective and differential medium for the detection of Enterobacteriaceae and certain Nonfermenters from clinical and non-clinical specimens. DCLS Agar, modified // Hektoen Enteric Agar DCLS Agar is a moderately selective differential medium for isolation of Salmonella, Shigella, and cholera vibrios from clinical and other specimens. Hektoen Enteric Agar is a moderately selective and differential medium for the isolation, cultivation and differentiation of gram-negative enteric microorganisms from both clinical and nonclinical specimens. It is of particular importance as a medium for the isolation of Shigella and Salmonella species. Group A Selective Strep Agar with 5 % Sheep Blood (BD ssa ) // BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) Group A Selective Strep Agar with 5 % Sheep Blood (ssa ) is recommended as a primary selective plating medium for the isolation of group A streptococci (S. pyogenes) from throat cultures and other specimens in which the presence of S. pyogenes is suspected. Group B streptococci will also grow on this medium; most other streptococci, neisseriae, staphylococci and gram-negative bacteria are inhibited. BD Trypticase TSA II is used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. MacConkey Agar // Columbia CNA Agar with 5 % Sheep Blood Mac Conkey Agar in combination with Columbia CNA Agar with 5 % Sheep Blood (Bi-Plate) is used for the isolation of Gram negative and Gram positive bacteria from clinical specimens. Middlebrook 7H10 // 7H11 Selective Agar Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. These bi-plates provide two media, one moderately selective and one highly selective, for the recovery of mycobacteria from specimens containing mixed flora. The Middlebrook 7H11 Agar is contained in the sector differentiated by the Roman numeral "I" molded into the bottom of the sector. The 7H11 Selective Agar is contained in the sector marked "II." Sabouraud Glucose Agar with Gentamycin and Chloramphenicol // BD CHROMagar Candida Sabouraud Glucose Agar in combination with BD CHROMagar Candida (Bi-Plate) is used for the isolation of fungi and for the isolation and identification of Candida species from clinical specimens. BD Diagnostics Diagnostic Systems Catalog 2008/

78 Prepared Media Prepared Plated Media (PPM) PPMs (Bi-Plates) Schaedler Agar // Schaedler Agar KV (Vitamin K1, Kanamycin, Vancomycin) With 5 % Sheep Blood. Schaedler Agar in combination with Schaedler KV Agar with 5 % Sheep blood (Bi-Plate) is used for the non-selective isolation of anaerobes and for the selective isolation of fastidious gram-negative anaerobic microorganisms, especially Bacteroides and Prevotella species and a variety of other gram-negative anaerobes. BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) // MacConkey II Agar BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) is a nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials and the detection of hemolytic reactions. MacConkey II Agar is a selective and differential medium for the detection of Enterobacteriaceae and certain Nonfermenters from clinical and non-clinical specimens. BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) // MacConkey II Agar with MUG BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) is a nutritious general purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials and the detection of hemolytic reactions. MacConkey II Agar with MUG is used for the presumptive identification of Escherichia coli. XLD Agar // Brilliant Green Agar, Modified Brilliant Green Agar Modified and XLD Agar in biplate format are used for isolating Salmonella and Shigella from water, sewage and foodstuffs or clinical specimens BD Diagnostics Diagnostic Systems Catalog 2008/2009

79 Prepared Media Prepared Plated Media (PPM) BD CHROMagar Plates BD CHROMagar Candida BD CHROMagar Candida medium is for the isolation and differentiation of Candida albicans, C. tropicalis and C. krusei. Due to the differences in morphology and colors of the yeast colonies, this medium facilitates the detection of mixed yeast cultures in specimens. It may also be used as a selective isolation medium for other yeasts and for filamentous fungi instead of Sabouraud Dextrose Agar or similar media. BD CHROMagar Listeria (100 mm) A selective medium for the isolation, differentiation, and presumptive identification of Listeria monocytogenes from food and environmental samples. BD CHROMagar MRSA A selective and differential medium primarily used for the qualitative and direct detection of colonization by methicillin resistant Staphylococcus aureus (MRSA) to aid in the prevention and control of MRSA infections. The test is performed on anterior nares, skin and throat specimens. The medium may also be used for the detection of MRSA from clinical specimens. Rapid results in as little as 24 hours without confirmatory testing. BD CHROMagar O157 BD CHROMagar O157 is a chromogenic medium for the selective isolation and presumptive identification of E. coli O157:H7 strains from clinical, veterinary, food, and environmental sources. BD CHROMagar Orientation BD CHROMagar Orientation is a non-selective medium for the direct identification, differentiation and enumeration of urinary tract pathogens. BD CHROMagar Salmonella BD CHROMagar Salmonella is a selective differential medium for the isolation and presumptive identification of Salmonella directly from stool specimens and from enrichments such as Selenite broth. It may also be used for the isolation of Salmonella from specimens other than feces such as food and water. BD CHROMagar Staph aureus BD CHROMagar Staph aureus Medium is a selective differential medium for the isolation and identification of Staphylococcus aureus without the use of confirmatory testing from clinical sources. Plates - Specials BBL mei Agar (60x15mm) BBL mei Agar is a selective culture medium used for the chromogenic detection and enumeration of enterococci in water by the single-step membrane filtration technique. It conforms with the U.S. Environmental Protection Agency (USEPA) Approved Method 1600: Enterococci in Water by Membrane Filtration Using membrane-enterococcus Indoxyl-ß-D-Glucoside Agar (mei). BD Diagnostics Diagnostic Systems Catalog 2008/

80 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm tubes x 8 ml tubes tubes tubes x 5 ml x 5 ml x 8 ml slants tubes tubes x 9 ml x 5 ml Acetamide Agar Acetamide Agar is used in the differentiation of nonfermentative gram-negative bacteria, particularly Pseudomonas aeruginosa. Prepared Slants, Tubes Size K. Alkaline Peptone Water Alkaline Peptone Water is an enrichment medium used for the cultivation of Vibrio species from feces and other infected materials. Clinical materials containing small numbers of Vibrio species should be inoculated into an enrichment medium prior to plating onto a selective medium, such as TCBS Agar. Alkaline Peptone Water is a suitable enrichment broth for this purpose. The relatively high ph of the medium (approximately 8.4) provides a favorable environment for the growth of Vibrio species. Prepared Tubes, 8 ml, Size D. Bile Esculin Agar Bile Esculin Agar is used to differentiate enterococci and the Streptococcus bovis group from other streptococci. Prepared Slants. Tubes Size K. Brain Heart Infusion Agar with 10 % Sheep Blood Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. With the addition of 5 % or 10 % sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species, including systemic fungi from clinical and nonclinical sources. Prepared Slants, Tubes Size K. Brain Heart Infusion Broth For the cultivation of aerobic and anaerobic microorganisms. Prepared Tubes, 5 ml and 8 ml, Size K. Brain Heart Infusion CC Agar with 10 % Sheep Blood and Gentamicin Brain Heart Infusion (BHI) Agar is recommended as a general-purpose medium for aerobic bacteriology and for the primary recovery of fungi from clinical specimens. With 10 % sheep blood, it is used to isolate systemic fungi that may grow poorly on the nonenriched medium. The presence of the antimicrobial agents, cycloheximide and/or chloramphenicol and, in modified formulations, gentamicin, penicillin and streptomycin, inhibits the growth of a wide variety of bacteria and fungi and enhances the isolation of pathogenic fungal species. Prepared Slants, Tubes Size C. Brain Heart Infusion with 0.1 % Agar A general-purpose media used in the cultivation of fastidious and nonfastidious microorganisms, especially anaerobic bacteria. BHI with 0.1 % Agar is for the cultivation of anaerobes because of the reduced oxygen tension of the medium. Prepared Tubes, Size D. Brain Heart Infusion with 6,5 % Sodium Chloride Prepared Tubes, Size K. Brain Heart Infusion with Fildes Enrichment A general purpose medium used in the cultivation of nonfastidious and fastidious microorganisms, particularly Haemophilus species, from a variety of clinical and nonclinical materials. Prepared Tubes, 9 ml, Size K. Campylobacter Thioglycollate Medium with 5 Antimicrobics Campylobacter Thioglycollate Medium with 5 Antimicrobics is recommended as a holding medium for samples suspected to contain Campylobacter jejuni subsp. jejuni when immediate inoculation of Campylobacter Agar with 5 Antimicrobics and 10 % Sheep Blood cannot be performed. Prepared Tubes, 5 ml, Size K BD Diagnostics Diagnostic Systems Catalog 2008/2009

81 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm slants tubes x 8 ml x 8 ml x 9 ml x 8 ml tubes tubes tubes x 9 ml Slants tubes slants tubes Chocolate II Agar (GC II Agar with Hemoglobin and BD IsoVitaleX ) Chocolate II Agar is an improved medium for use in qualitative procedures for the isolation and cultivation of fastidious microorganisms, especially Neisseria and Haemophilus species, from a variety of clinical specimens. Media provided in divided (two-sectored) plates offer the ability to utilize the properties of two media in one plate. Prepared Slants, Tubes Size K. Chopped Meat Carbohydrate Broth (PR II) Pre-reduced medium used in the enrichment, cultivation and maintenance of anaerobic microorganisms, particularly obligate anaerobes. Prepared Tubes (Hungate cap), Size K. Cooked Meat Medium For the cultivation of anaerobic bacteria and maintenance of stock cultures, especially pathogenic clostridia. Prepared Tubes, 8 ml, Size K. Cooked Meat Medium with Glucose, Hemin and Vitamin K1 Cooked Meat Medium and the enriched medium are used for the cultivation of anaerobes, especially pathogenic clostridia. Prepared Tubes, 9 ml, Size K. BD CTA Medium BD CTA Medium (Cystine Tryptic Agar) supports the growth of fastidious microorganisms and detection of bacterial motility. Differentiation of fastidious organisms by means of fermentation reactions can be achieved through the addition of carbohydrates : Prepared Tubes, 8 ml, Size K : Prepared Tubes with DEXTROSE, Tubes Size K : Prepared Tubes with LACTOSE, Tubes Size K : Prepared Tubes with MALTOSE, Tubes Size K D/E Neutralizing Broth For hygiene control testing; inactivates disinfecting agents. Prepared Tubes, 9 ml, Size A. Dermatophyte Test Medium, Modified with Chloramphenicol Dermatophyte Test Medium (DTM) is a selective and differential medium used for the detection and presumptive identification of dermatophytes from clinical and veterinary specimens. Because of the unavailability of one of the inhibitory agents, chlortetracycline, Dermatophyte Test Medium (DTM), Modified with Chloramphenicol is recommended as a substitute for the original DTM formation. Prepared Slants, Tubes Size C. Dubos Broth, Enriched Used for the cultivation of pure cultures of M. tuberculosis. Prepared Tubes, Size K. BD Enterococcosel Agar BD Enterococcosel Agar, a Bile Esculin Agar with Azide, is used for the rapid, selective detection and enumeration of enterococci. Prepared Slants, Tubes Size K. BD Enterococcosel Broth BD Enterococcosel Broth is a selective medium for the cultivation and differentiation of enterococci. Prepared tubes of BD Enterococcosel Broth are manufactured from pretested BD BBL dehydrated base in prepared Tubes, Size K. BD Diagnostics Diagnostic Systems Catalog 2008/

82 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm x 8 ml x 8 ml tubes tubes tubes tubes tubes x 5 ml slants slants x 10 ml x 10 ml x 5 ml slants GN Broth Gram-Negative (GN) Broth is a selective enrichment medium for the cultivation of gram-negative enteric organisms. Prepared tubes of GN Broth are manufactured from pretested brand dehydrated base in K tubes. Prepared Tubes, 8 ml, Size K. Gottsacker TB Medium Solid egg-based media for the recovery and culture of Mycobacteria. Prepared Tubes, Size A. Herrold's Egg Yolk Agar with Mycobactin J and ANV Used for the selective isolation and differentiation of Mycobacterium paratuberculosis. ANV (amphotericin, nalidixic acid and vancomycin) inhibits contamination and Mycobactin J is a growth factor for M. paratuberculosis. Prepared Tubes, Size C. Herrold's Egg Yolk Agar without Mycobactin J with ANV Used for the selective isolation and differentiation of mycobacteria other than Mycobacterium paratuberculosis. ANV (amphotericin, nalidixic acid and vancomycin) inhibits contamination. M. paratuberculosis is unable to grow on media lacking Mycobactin J. (For use with Herrold's Egg Yolk Agar with Mycobactin J and ANV, Cat. Nos or ). Prepared Tubes, Size C. Jordan's Tartrate Agar Used as an aid in the Identification of members of the Enterobacteriaceae on the basis of tartrate utilization. Agar Deeps, 5 ml, Tubes Size K. Kligler Iron Agar Kligler Iron Agar is used for the differentiation of members of the Enterobacteriaceae family based on of their ability to ferment dextrose and lactose and to liberate sulfides. Specimen Type: Fecal Specimens. Prepared Slants, Size K. Lactose Broth with Durham Tube (Double Strength) Lactose Broth is used for detection of the presence of coliform organisms, as a pre-enrichment broth for salmonellae and in the study of lactose fermentation of bacteria in general. Lactose Broth with Durham Tube, 10 ml, Tubes Size A (Double Strength). Lauryl Sulfate Broth with Durham Tube (Double Strength) Lauryl Tryptose Broth and Lauryl Sulfate Broth, which are also known as Lauryl Sulfate Tryptose (LST) Broth, are used for the detection of coliform organisms in materials of sanitary importance. With Durham Tube, 10 ml, Tubes Size A (Double Strength). LIM Broth (Todd Hewitt with CNA) Lim Broth us used for the selective enrichment of group B streptococci (Streptococcus agalactiae), especially from genital specimens. Prepared Tubes, 5 ml, Size K. Loeffler Medium Used for the cultivation and morphological characterization of corynebacteria. This media, which includes horse serum, meat infusion and dextrose, has a gray-white surface that provides an excellent background for the detection of colonial pigmentation (by using the incorporated methylene blue dye). Specimen Type: Throat. Prepared Slants, Size K BD Diagnostics Diagnostic Systems Catalog 2008/2009

83 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm deeps slants slants slants slants bottles slants tubes tubes slants slants tubes tubes x 5 ml slants slants slants Loewenstein-Jensen Medium For Semi-Qualitative Catalase Test. Loewenstein-Jensen Medium Deeps contain an enriched eggbased medium and are used for the cultivation of mycobacteria, including Mycobacterium tuberculosis. Deeps are tubes of hardened agar medium which are inoculated by "stabbing" the inoculum into the agar. Used for for Semi-Qualitative Catalase Test : Prepared Deeps, Tubes Size A & : Prepared Slants, Tubes Size A & : Prepared Slants, Tubes Size C Loewenstein-Jensen Medium (BD Mycoflask ) An enriched egg-based medium used for the cultivation of Mycobacterium tuberculosis (causative agent of tuberculosis) and other mycobacterial species. The long shelf life of this product is ideal for laboratories that process only a few specimens. This medium comes in a BD Mycoflask Bottle. Loewenstein-Jensen Medium with 5 % Sodium Chloride L-J Medium Slants with 5 % NaCl are intended for the isolation and cultivation of mycobacteria that can tolerate 5 % Sodium chloride (e.g. M. fortuitum and M. chelonae subsp. absessus). Prepared Slants, Tubes Size C. Loewenstein-Jensen Medium with Glycerol and PACT Loewenstein-Jensen Medium Slants with Glycerol and PACT are used for the cultivation of mycobacteria, especially M. tuberculosis. It is a selective medium containing polymyxin, amphotericin, carbenicillin, and trimethoprim (PACT). Prepared Tubes, Size A. Lysine Iron Agar Used for the differentiation of enteric organisms based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulfide. Prepared slants, Tubes Size K. MacConkey II Agar with Sorbitol Used as a selective and differential medium for the detection of Escherichia coli serotype O157:H7 associated with hemorrhagic colitis. Prepared Tubes (Pour Tubes), Size A. Malonate Broth, Ewing Modified Malonate Broth, as modified by Ewing, is used for the differentiation of coliforms and other enteric organisms. Prepared Tubes, Size K. Middlebrook 7H9 Broth with Glycerol Middlebrook 7H9 Broth with Glycerol is a nonselective liquid culture medium for the cultivation of mycobacteria, including M. tuberculosis. It is used primarily for growth of pure cultures of mycobacteria for use in laboratory studies. Prepared Tubes, 5 ml, Size K. Middlebrook and Cohn 7H10 Agar Middlebrook and Cohn 7H10 Agar is a culture medium for the cultivation of mycobacteria, especially mycobacteria other than tuberculosis (MOTT) : Prepared Slants, Tubes Size A : Prepared Slants, Tubes Size A : Prepared Slants, Tubes Size C BD Diagnostics Diagnostic Systems Catalog 2008/

84 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm x 5 ml x 5 ml x 5 ml x 5 ml x 5 ml tubes x 5 ml x 5 ml slants x 20 ml slants Moeller Decarboxylase Broth Base with Lysine Decarboxylase media are used in the biochemical differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase and lysine and ornithine decarboxylase. Decarboxylase Medium Base, with added arginine, lysine or ornithine is used for the same purpose. LYSINE Decarboxylase Broth is used for differentiating microorganisms based on lysine decarboxylation. Prepared Tubes with LYSINE, 5 ml, Size K. Moeller Decarboxylase Broth with Ornithine Decarboxylase media are used in the biochemical differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase and lysine and ornithine decarboxylase. Decarboxylase Medium Base, with added arginine, lysine or ORNITHINE is used for the same purpose. Prepared Tubes with ORNITHINE, 5 ml, Size K. Motility Indole Ornithine Medium (MIO Medium) Motility Indole Ornithine (MIO) Medium is used to demonstrate motility, indole production and ornithine decarboxylase activity for the differentiation of Enterobacteriaceae. Prepared Deeps, 5 ml, Tubes Size K. Motility Test Medium Motility Test Medium is used for the detection of motility of gram-negative enteric bacilli. Prepared Tubes, Size K. Mueller Hinton Broth Mueller Hinton Broth is a general purpose medium that may be used in the cultivation of a wide variety of fastidious and nonfastidious microorganisms. This medium is not supplemented with calcium or magnesium ions. Prepared Tubes, 5 ml, Size C. Mueller Hinton II Broth Mueller Hinton II Broth (Cation-Adjusted) is intended for use in quantitative procedures for susceptibility testing of rapidly-growing aerobic and facultatively anaerobic bacteria isolated from clinical specimens. It is formulated to have a low thymine and thymidine content and is adjusted to the calcium and magnesium ion concentrations recommended in CLSI Standard, formerly NCCLS, M7. Prepared Tubes, 5 ml, Size K. BD Mycosel Agar BD Mycosel Agar is a highliy selective medium containing cycloheximide and chloramphenicol. It is recommended for the isolation of pathogenic fungi from materials having a large amount of flora of other fungi and bacteria. Prepared Slants, Tubes Size A. Nutrient Agar Nutrient Agar is used for the cultivation of bacteria and for the enumeration of organisms in water, sewage, feces and other materials : Prepared Deeps (Pour Tubes), 20 ml, Tubes Size A : Prepared Slants, Tubes Size K x 5 ml x 5 ml Nutrient Broth Nutrient Broth is used for the cultivation of many species of nonfastidious microorganisms. Prepared Tubes, 5 ml, Size K. OF Basal Medium OF (Oxidation Fermentation) media are used for the determination of oxidative and fermentative metabolism of carbohydrates by gra-negative rods on the basis of acid reaction in either the open or closed system. Prepared Tubes, 5 ml, Size K BD Diagnostics Diagnostic Systems Catalog 2008/2009

85 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm tubes tubes x 100 ml slants tubes tubes slants tubes tubes tubes tubes x 0.5 ml tubes slants x 20 ml OF Basal Medium with Dextrose OF media are used for the determination of oxidative and fermentative metabolism of carbohydrates by gram-negative rods based on acid reactions. Prepared Tubes with DEXTROSE, Size K. Orange Serum Broth Concentrate (10 x) Used for cultivating aciduric microorganisms, particularly those associated with spoilage of citrus products. Each of the 6 ampules contains 100 ml of 10 x concentrate. Petragnani Medium Used in qualitative procedures for the isolation and cultivation of mycobacteria from clinical specimens. Prepared Slants, Tubes Size C. Phenol Red Broth with Dextrose and Durham Tube Phenol Red Broth Base, when supplemented with an appropriate carbohydrate, is used to determine the fermentation activities of microorganisms. Prepared tubes of the basal medium and the medium supplemented with one of the eighteen carbohydrates listed above are manufactured from pretested dehydrated base in K tubes containing Durham tubes. Prepared Tubes with DEXTROSE, Size K. Phenol Red Broth with Xylose and Durham Tube Phenol Red Broth Base and with Phenol Red Broth carbohydrates are used for the determination of fermentation reactions in the differentiation of microorganisms. Prepared Tubes with XYLOSE, Size K. Phenylalanine Agar Phenylalanine Agar is used for the differentiation of enteric bacilli on the basis of their ability to produce phenylpyruvic acid by oxidative deamination. Ferric Chloride Reagent is used to visualize the phenylalanine deamination reaction. Prepared Slants, Tubes Size K. Rapid Fermentation Medium Rapid Fermentation Medium and Rapid Fermentation Medium with Carbohydrates provide a rapid method for the differentiation of Neisseria species isolated from clinical specimens. Prepared Tubes, Size K : with DEXTROSE; : with LACTOSE; : with MALTOSE; : with SUCROSE; Rapid Urea Broth Used for the presumptive identification of Helicobacter pylori in gastric antral biopsy specimens. Prepared Vial, 0.5 ml. Rappaport-Vassiliadis Broth (RV Broth) Selective enrichment broth. For Salmonella from food and water. Prepared Tubes. Sabouraud Brain Heart Infusion Agar with Chloramphenicol and Gentamicin Sabouraud Brain Heart Infusion Agar is used in qualitative procedures for cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. The medium is rendered selective by the addition of antimicrobial agents. Prepared Slants, Tubes Size C. Sabouraud Dextrose Agar Sabouraud Dextrose Agar is a general purpose medium devised by Sabouraud fro the cultivation of dermatophytes. Prepared Agar Deeps (Pour Tube), 20 ml, Tubes Size A. BD Diagnostics Diagnostic Systems Catalog 2008/

86 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm slants slants slants slants x 8 ml x 5 ml x 5 ml x 10 ml Sabouraud Dextrose Agar with Chloramphenicol The addition of Chloramphenicol is a modification designed to increase bacterial inhibition and enable the isolation from contaminated specimens of opportunistic fungi that cause clinical infections resembling dermatophytosis but are sensitive to the cycloheximide included in some selective fungal media. Prepared Slants, Tubes Size C. Sabouraud Dextrose Agar (ph 5.6) The low ph of approx. 5.6 is faborable for the growth of fungi, especially dermatophytes, and slightly inhibitory to contaminating bacteria in clinical specimens. Prepared Slants, Size A. Sabouraud Dextrose CC Agar Sabouraud Dextrose Agar with Chloramphenicol and Cycloheximide; Used in qualitative procedures for the cultivation of dermatophytes and other pathogenic and nonpathogenic fungi from clinical and non-clinical specimens. Prepared Slants, Tubes Size A. Sabouraud Liquid Broth, Modified (Antibiotic Assay Medium 13) Sabouraud Liquid Broth is used in qualitative procedures for cultivation of dematophytes and other pathogenic and nonpathogenic fungi from clinical and nonclinical specimens. Contains antimicrobial agents that render the media selective. Prepared Tubes, 8 ml, Size K. Saline, Normal Saline, Normal (physiological) is used in procedures that require the use of an isotonic diluent. It is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability : Prepared Tubes, 5 ml, Size K : Prepared Tubes, 5 ml, Size K : Prepared Tubes, 10 ml, Size D tubes tubes x 8 ml x 8 ml x 8 ml slants slants Schaedler Broth with Vitamin K1 Used for the cultivation of fastidious aerobic and anaerobic microorganisms. Prepared Tubes, Size K. Selenite Cystine Broth Used as a selective enrichment medium for the isolation of Salmonella from feces, foods, pharmaceutical articles, water and other materials of sanitary importance. Prepared Tubes, 8 ml, Size K. Selenite-F Broth Used as an enrichment medium for the isolation of Salmonella and some species of Shigella from fecal, urine, water, and food samples. Prepared Tubes, 8 ml, Size K. Serum Tellurite Agar Serum Tellurite Agar is a selective and differential medium used for isolation of members of the genus Corynebacterium, particularly in the laboratory diagnosis of diphtheria. Prepared Slants, Tubes Size K. Seven H11 Agar (Middlebrook 7H11 Agar) Seven H11 Agar (also referred to as Middlebrook 7H11 Agar). The media is used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Prepared Slants, Tubes Size A BD Diagnostics Diagnostic Systems Catalog 2008/2009

87 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm slants tubes tubes tubes slants x 20 ml x 5 ml tubes tubes x 10 ml x 10 ml x 10 ml Seven H11 Agar with Aspartic Acid and Sodium Pyruvate Used for isolation and identification of nonchromogenic mycobacteria, especially M. tuberculosis. The aspartic acid is included to increase the rate of niacin production. Prepared Slants, Tubes Size A. SF Broth SF (Streptococcus Faecalis) Medium (Broth) is used for the differentiation of Enterococcus species from the Steptococcus bovis group and other streptococci. Prepared Tubes, Size K. SIM Medium SIM (Sulfide, Indole, Motility) Medium is used to differentiate enteric bacilli on the basis of sulfide production, indole formation and motility. Sulfide production is detected by a black precipitate. Indole formation is detected by the addition of Kovacs' reagent resulting in a red color. Motility detection is noted by growth radiating out from the central stab line that indicates that the test organism is motile. Specimen Type: All Specimens. Prepared Tubes, Size K. Simmons Citrate Agar Simmons Citrate Agar is used for the differentiation of gram-negative bacteria on the basis of citrate utilization. A positive reaction is indicated by growth with an intense blue color in the slant. A negative reaction is evidenced by no growth with no change in color (remains dark green). Specimen Type: All Specimens. Prepared Slants, Size K. Standard Methods Agar Plate Count Agar and Standard Methods Agar (Plate Count Agar; Tryptone Glucose Yeast Agar) are used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance. Prepared Agar Deeps (Pour Tube), 20 ml (TGY, for Plate Count), Tubes Size A. Sterile Water Sterile Water may be used in laboratory procedures; e.g., preparation of dilutions of reagents and suspensions of microorganisms. Prepared Tubes, 5 ml, Size K. Stonebrink TB Medium with PACT Stonebrink TB Medium with PACT Selective Medium is intended for the isolation of mycobacteria, especially Mycobacterium tuberculosis. It is a selective medium containing Polymyxin, Amphotericin, Carbenicillin, and Trimethoprim (PACT). Prepared Tubes, Size A. Tetrathionate Broth Base Tetrathionate Broth Base, with added iodine-iodide solution, is used as a selective enrichment medium for the isolation of Salmonella from feces, urine, foods and other materials of sanitary importance. Prepared Tubes, 10 ml, Size D. Thioglycollate Medium without Indicator - 135C Thioglycollate Medium without Indicator (135C) is an enriched general-purpose medium for the recovery of a wide variety of microorganisms, particularly obligate anaerobes, from clinical specimens and other materials. Prepared Tubes, 10 ml, Size D. Thioglycollate Medium, Enriched with Calcium Carbonate A general-purpose Medium used in qualitative procedures for the cultivation of fastidious as well as nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical materials. It is also recommended for the maintenance of stock cultures. Prepared Tubes, 10 ml, Size D. BD Diagnostics Diagnostic Systems Catalog 2008/

88 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm tubes x 5 ml x 5 ml x 8 ml x 8 ml tubes x 8 ml x 8 ml x 18 ml x 5 ml x 5 ml tubes tubes tubes tubes slants BBL Fluid Thioglycollate Medium Fluid Thioglycollate Medium (FTM) is used for the sterility testing of biologics and for the cultivation of anaerobes, aerobes and microaerophiles. Prepared tubes, 20 ml, size A. Thioglycollate Medium, Enriched with Vitamin K1 and Hemin Enriched Thioglycollate Medium is a general-purpose media used in qualitative procedures for the cultivation of fastidious, as well as nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical specimens. Prepared Tubes, 5 and 8 ml, Size K. Thioglycollate Medium, Enriched with Vitamin K1 and Hemin Enriched Thioglycollate Medium is a general-purpose media used in qualitative procedures for the cultivation of fastidious, as well as nonfastidious microorganisms, including aerobic and anaerobic bacteria, from a variety of clinical and nonclinical specimens. Prepared Tubes, Size K. Thioglycollate Medium, Fluid Fluid Thioglycollate Medium (FTM) is used for the sterility testing of biologics and for the cultivation of anaerobes, aerobes and microaerophiles : Prepared Tubes, 10 x 8 ml, Size K : Prepared Tubes, 100 x 8 ml, Size K : Prepared Tubes, 100 x 18 ml, Size A Todd Hewitt Broth Todd Hewitt Broth is a general-purpose medium, which primarily is used for the cultivation of betahemolytic streptococci, especially for serological studies. Prepared Tubes, 5 ml, Size K. Todd Hewitt Broth with Gentamicin and Nalidixic Acid Todd Hewitt Broth with Gentamicin and Nalidixic Acid is used for the selective enrichment of group B streptococci (Streptococcus agalactiae), especially from genital specimens. Prepared Tubes, Size K. BD Trichosel Broth, Modified with 5 % Horse Serum BD Trichosel Broth, Modified is used for the isolation and cultivation of Trichomonas species. It is a modification of the simplified Trypticase Serum (STS) Medium of Kupferberg et al. for the cultivation of Trichomonas spp. The classical formula has been modified by the addition of beef extract and horse serum and an increased amount of yeast extract to improve performance. Chloramphenicol, a relatively stable antibiotic, replaces the penicillin and streptomycin recommended for addition to the STS base. Prepared Tubes, Size E. BD Trypticase Soy Agar Tryptic (BD Trypticase ) Soy Agar (TSA) is used for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes. Prepared Tubes, Size K. BD Trypticase Soy Agar with Defibrinated Sheep Blood (Blood Agar Slants) Tryptic Soy Blood Agar Base No. 2, Tryptic Soy Blood Agar Base EH and BD Trypticase Soy Agar, Modified (TSA II) supplemented with blood are used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. Prepared Slants, Tubes Size K BD Diagnostics Diagnostic Systems Catalog 2008/2009

89 Prepared Media Prepared Tubed Media (PTM) Liquid & Solid Media in Prepared Tubes Tube sizes including caps: A = 19.5 x 145 mm; C = 19.5 x 113 mm; K = 16.5 x 102 mm x 20 ml x 20 ml x 18 ml x 21 ml x 2 ml x 5 ml x 5 ml x 8 ml x 8 ml x 10 ml x 1.5 ml tubes slants slants slants slants tubes tubes x 3 ml BD Trypticase Soy Agar, Modified (TSA II) Tryptic Soy Blood Agar Base No. 2, Tryptic Soy Blood Agar Base EH and BD Trypticase Soy Agar, Modified (TSA II) supplemented with blood are used for cultivating fastidious microorganisms and for the visualization of hemolytic reactions produced by many bacterial species. Agar Deeps (Pour Tubes), 20 ml, Size A. BD Trypticase Soy Broth : Prepared Tubes, 100 x 18 ml, Size A : Prepared Tubes, 100 x 21 ml, Size A : Prepared Tubes, 10 x 8 ml, Size K : Prepared Tubes, 100 x 8 ml, Size K : Prepared Tubes, 10 x 5 ml, Size K : Prepared Tubes, 100 x 5 ml, Size K : Prepared Tubes, 100 x 2 ml, Size K : Prepared Tubes, 100 x 10 ml, Size D BD Trypticase Soy Broth with 20 % Glycerol For Stock Culture Preservation. Prepared Tubes, 1.5 ml, Size K. BD Trypticase Soy Broth with 6.5 % Sodium Chloride BD Trypticase Soy Broth with 6.5 % Sodium Chloride is used to differentiate Enterococcus spp. from the Streptococcus bovis group of streptococci. Prepared Tubes, Size K. TSI Agar Triple Sugar Iron Agar is used for the differentiation of gram-negative enteric bacilli based on carbohydrate fermentation and production of hydrogen sulfide. Specimen Type: Stool. Prepared Slants, Size K. Urea Agar Urea Agar is used for the differentiation of organisms, especially Enterobacteriaceae, on the basis of urease production. The production urease is indicated by an intense pink-red (fuchsia) color on the slant. A negative reaction is no color change; the agar medium remains pale yellow to buff colored. Specimen Type: All Specimens. Prepared Slants, Size K. Urea Agar Base Concentrate (10 ) Urea Agar Broth is used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Prepared Tubes, Size K. Urease Broth Concentrate (10 ) Prepared Tubes, Size K. Urease Test Broth For the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Prepared Tubes, 3 ml, Size K. BD Diagnostics Diagnostic Systems Catalog 2008/

90 Prepared Media BD Prepared Bottled Media (PBM) Liquid & Solid Media in Prepared Bottles x 200 ml x 200 ml x 250 ml x 900 ml x 700 ml x 90 ml x 100 ml x 500 ml x 100 ml x 15 ml x 4.5 ml x 10 ml x 100 ml x 100 ml Antibiotic Medium 10 Antibiotic Medium 11 (ph 8) Antibiotic Medium 19 Antibiotic Medium 19 is a partially completed medium used in the cylinder plate method assay for the determination of the activity of antimycotics (amphotericin B, natamycin, and nystatin). 250 ml Medium in 300 ml Sirup Bottle with Screw Cap. Brain Heart Infusion Broth General-purpose liqued medium used in the cultivation of fastidious and nonfastidious microorganisms. Buffered Peptone Water Buffered Peptone Water is used for preenriching injured Salmonella species from food specimens to increase recovery. 700 ml Medium in 1000 ml Sirup Bottle with Screw Cap. Buffered Peptone Water with 0.1 % Tween 90 ml solution in a 250 ml Infusion Bottle with blue flip/tear-off seal. Buffered Sodium Chloride-Peptone Solution (ph 7.0) Used for dissolving, suspending, and diluting test samples according to the European Pharmacopoeia (EP) : 25 x 100 ml Medium in 100 ml Infusion Bottle with injection stopper and pull ring; : 10 x 500 ml Medium in 500 ml Infusion Bottle with injection stopper and pull ring; D/E Neutralizing Broth D/E Neutralizing Broth is for the neutralization and testing of antiseptics and disinfectants according to the procedure of Engley and Dey. 100 ml Medium in 125 ml Sirup Bottle with Screw Cap. Dermatophyte Test Medium Agar Dermatophyte Test Medium Agar is a selective medium for the isolation of pathogenic fungi from superficial infections such as skin, hair, and nails. It is made available as slants in screw-cap vials. Prepared Slants (15 ml) in 30 ml screw-cap vials. Dorset Egg Medium, Modified Dorset Egg Medium, Modified is a prepared tubed, slanted, coagulated egg medium used for cultivation, maintenance and transport of pure cultures of mycobacteria and other fastidious and nonfastidious organisms. Prepared Slants (4.5 ml) in 15 ml screw-cap vials. Enterobacteriaceae Enrichment Broth (EE Broth Mossel) For the selective enrichment and detection of Enterobacteriaceae, especially in food. 10 ml Medium in 30 ml Screw Cap Vial. EE Broth Used for selectively enriching and detecting Enterobacteriaceae, particularly from foods : 100 ml Medium in 125 ml Sirup Bottle with 28 mm Aluminium Screw cap : 100 ml Medium in 210 ml Wide Mouth Bottle with Twist-Off Cap BD Diagnostics Diagnostic Systems Catalog 2008/2009

91 Prepared Media BD Prepared Bottled Media (PBM) Liquid & Solid Media in Prepared Bottles x 100 ml x 400 ml x 700 ml x 300 ml x 300 ml x 10 ml x 90 ml x 100 ml Fluid A Fluid A is a diluting and rinsing fluid used for sterility tests of pharmaceutical products according to the membrane filtration method. Inactivating Solution For Enrichment and Inactivation of Desinfection Media and Preservatives. 300 ml Medium in 500 ml Infusion Bottle with Septum. Inactivating Solution For Enrichment and Inactivation of Desinfection Media and Preservatives. 300 ml Medium in 500 ml Infusion Bottle with Septum (double wrapped). Kirchner Medium For Isolation and Culture of Mycobacteria. 10 ml Medium in 28 ml Screw Cap Vial. Lactose Broth Used for detection of the presence of coliform organisms, as a pre-enrichment broth for salmonellae and in the study of lactose fermentation of bacteria in general. Selective medium for detection of gram-negative, lactose fermenting coliforms and salmonella in water and foods : 90 ml Medium in 210 ml Wide Mouth Jar with Screw Cap : 100 ml Medium in 380 Wide Mouth Jar with 66 mm Metal Twist-Off Cap x 100 ml x 90 ml x 850 ml x 450 ml x 50 ml x 50 ml x 100 ml x 90 ml x 100 ml x 100 ml Lactose Broth (Double Strength) 100 ml Medium in 380 ml Wide Mouth Jar with Screw Cap. Lactose Broth with Stopper 90 ml Medium in 250 ml Infusion Bottle with with flip/tear-off seal and injection stopper. LB Broth (Miller) LB (Luria-Bertani) Broth, Miller is used for maintaining and propagating Escherichia coli in molecular microbiology procedures : 850 ml Medium in 1000 ml Sirup Bottle with Screw Cap : 450 ml Medium in 500 ml Sirup Bottle with Screw Cap. Levinthal Agar Base - double sterile Filling volume 50 ml; in 125 ml sirup bottle with 28 mm aluminium screw cap. Levinthal Stock Broth Filling volume 50 ml; in 125 ml sirup bottles with 28 mm aluminium screw cap. Letheen Broth, Modified For the microbiological testing of cosmetics. 90 ml resp. 100 ml Medium in a wide mouth jar with twist-off cap; MacConkey Broth For cultivating gram-negative, lactose-fermenting bacilli in water and foods. 100 ml Medium in 210 ml Wide Mouth Jar with Durham tube and Screw Cap. MacConkey Broth with Durham Tube 100 ml Medium in 125 ml Sirup Bottle with Durham tube and Screw Cap. BD Diagnostics Diagnostic Systems Catalog 2008/

92 Prepared Media BD Prepared Bottled Media (PBM) Liquid & Solid Media in Prepared Bottles x 5 ml x 500 ml x 4 ml x 10 ml x 500 ml x 450 ml x 10 ml x 100 ml x 250 ml x 400 ml Nutrient Broth Nutrient Broth is used for the cultivation of many species of nonfastidious microorganisms. 5 ml Medium in 8 ml Vial with Aluminium Screw Cap. Peptone Water (0.1 %) : 300 ml in Prepared Bottle with Septum Screw Cap : 500 ml Medium in 500 ml Infusion Bottle : 500 ml Medium in 500 ml Sirup Bottle with Aluminium Screw Cap : 500 ml Medium in 500 ml Sirup Bottle Peptone Water with Glucose and Durham Tube 4 ml Medium in 8 ml Vial with Screw Cap and Durham Tube. Phosphate Buffered Saline (PBS) Phosphate Buffered Saline (PBS) is used in cold enrichment procedures to enhance the recovery of Yersinia enterocolitica. 10 ml Medium in 15 ml Screw Cap Vial. Bottle Phosphate Buffer Phosphate buffer, PH 7.2 USP 100ml Phosphate Buffered Saline (ph 7.0) For the preparation of dilution blanks for use in the examination of waters, dairy products, foods and other materials. 500 ml Medium in 1000 ml Sirup Bottle with Aluminium Screw Cap. Rappaport-Vassiliadis Medium, Modified (Semisolid) Rappaport-Vassiliadis (MSRV) Medium, Semisolid Modification is used with Novobiocin Antimicrobic Supplement in rapidly detecting motile Salmonella in feces and food products. 450 ml Medium in 500 ml Sirup Bottle with Aluminium Screw Cap. Rappaport-Vassiliadis Soy Peptone Broth Rappaport Vassiliadis Broth (=R10 Broth) is a liquid medium for selectively enriching Salmonella from meat and dairy products, feces and polluted water. 10 ml Medium in 30 ml Screw Cap Vial. Sabouraud Glucose Agar Sabouraud Glucose Agar, supplied in bottles, is a partially completed medium used for the isolation and cultivation of fungi (yeasts, molds, and dermatophytes) from clinical and nonclinical materials : 100 ml Medium in 150 ml Sirup Bottle : 250 ml Medium in 300 ml Falt Bottle with Screw Cap : 400 ml Medium in 500 ml graduated Laboratory Bottle x 10 ml x 12 ml Saline Solution (0.9 %) Used in microbiological procedures that require an isotonic or buffered diluent. They are mainly used for the suspension and dilution of non-fastidious microorganisms. 10 ml Medium in 15 ml Screw Cap Vial. Tetrathionate Broth For the isolation of Salmonella in fecal, urine, and food specimens. 12 ml Medium in 30 ml Screw Cap Vial BD Diagnostics Diagnostic Systems Catalog 2008/2009

93 Prepared Media BD Prepared Bottled Media (PBM) Liquid & Solid Media in Prepared Bottles x 100 ml x 150 ml x 800 ml ml x 500 ml x 250 ml x 500 ml Tetrathionate Broth Base Tetrathionate Broth Base, with added iodine-iodide solution, is used as a selective enrichment medium for the isolation of Salmonella from feces, urine, foods and other materials of sanitary importance. 100 ml Medium in 125 ml Sirup Bottle with 28 mm Aluminium Screw Cap. Thioglycollate Medium, Fluid 100 ml Medium in 150 ml Wide Mouth Jar with twist-off cap. Todd Hewitt Broth Todd Hewitt Broth is a general-purpose medium, which primarily is used for the cultivation of betahemolytic streptococci, especially for serological studies. 800 ml Medium in 1000 ml Sirup Bottle with 28 mm Aluminium Screw Cap. BD Tryptic Soy Agar Tryptic Soy Agar, provided in bottles, is a partially completed general purpose medium which, after pouring into Petri dishes or tubes, supports the growth of nonfastidious as well as moderately fastidious microorganisms. In clinical microbiology, it is not used for the isolation of pathogens from clinical specimens but may be used for cultivating bacterial strains. After supplementation with blood, it may be used as a primary isolation medium in clinical microbiology : 100 ml Medium in 250 ml Sirup Bottle : 400 ml Medium in 500 ml Laboratory Bottle with Graduation : 250 ml Medium in 500 ml Flat Bottle with Aluminium Screw Cap : 500 ml Medium in 500 ml Sirup Bottle with Screw Cap x 100 ml x 100 ml x 100 ml x 25 ml x 400 ml BD Trypticase Soy Broth Prepared Bottles, 100 ml Medium in Serum Bottle. Tryptic Soy Broth (Sterile ETO) Used for sterility testing in sterile filling rooms. After autoclaving the medium in the container, the whole package unit is sealed in a Stericlin bag and is sterilized by ethylene oxide (=ETO) treatment, providing a sterile outer surface of the containers and package. The multiple bags in these products allow the introduction of the containers into clean rooms from a non-sterile to a sterile area. 100 ml in 125 ml Infusion bottle with Flip Tear-Off Seal and Stopper Tryptic Soy Broth 100 ml Medium in 150 ml Wide Mouth Jar. Tween 80 (20 %) Emulsifying Agent for Lipids and Oils. 25 ml Medium in 30 ml Vial with Screw Cap. Water (Sterile, Double Wrapped) Not for Injection. 400 ml Medium in 500 ml Infusion Bottle with Septum. BD Diagnostics Diagnostic Systems Catalog 2008/

94 Prepared Media BD Prepared Slide Media for Urine Diagnostics Dip Slides for Urine Diagnostics slides slides slides slides slides slides slides Go Slide Go Slide is a set of dip slides and carbon dioxide tablets for the cultural detection of Neisseria gonorrhoeae with chocolate agar and Thayer Martin agar. Urotube Urotube is a set of dip slides for the cultivation and determination of the total bacterial count of gram-positive and gram-negative bacteria in urine. Suitable as a transport medium. Contains 3 slides, each with different agar media: CLED Agar for determining the total bacterial count, MacConkey Agar for selective growth of gram-negative bacteria, and Cetrimide agar for the selective growth of Pseudomonadaceae, especially Pseudomonas aeruginosa. Urotube - Bactrim SXT BBL Urotube Bactrim SXT is a dip slide for the determination of the total bacterial count in the urine and resistance control towards SXT. with 3 different agar media. Slide 1: CLED Agar, designed for the determination of the total bacterial count. Slide 2: MacConkey Agar, medium for the selective growth of gram-negative bacteria. Slide 3: Antagonism-free agar with SXT. Urotube - E BBL Urotube E is a set of dip slides for primary cultivation and determination of the colony count of gram-positive and gram-negative bacteria as well as for the screening and specific detection of enterococci in urine. Suitable as a transport medium. Contains 3 slides, each with different agar media: CLED Agar for determining the total bacterial count, MacConkey Agar for the selective growth of gram-negative bacteria, and Enterococci agar (with the substrate esculin) for the specific detection of enterococci. Urotube - E. coli BBL Urotube E. coli is a set of dip slides for primary cultivation and determination of the colony count of gram-positive and gram-negative bacteria as well as for the screening and specific detection of E. coli and Proteus species. Suitable as a transport medium. Contains 3 slides, each with different agar media: CLED Agar for determining the total bacterial count, MacConkey Agar for the selective growth of gram-negative bacteria, and beta-glucuronidase agar, which is an indicator medium for the growth and specific detection of E. coli and Proteus species. Urotube - M BBL Urotube M is a a set of dip slides for primary cultivation and determination of the colony count of gram-positive and gram-negative bacteria as well as for the screening and specific detection of yeasts and molds. Contains 3 slides, each with different agar media: CLED Agar for determining the total bacterial count, MacConkey Agar for the selective growth of gram-negative bacteria, and Malt agar to facilitate growth of yeasts and molds BD Diagnostics Diagnostic Systems Catalog 2008/2009

95 Prepared Media BD Prepared Slide Media for Urine Diagnostics Dip Slides for Yeast Detection slides slides slides BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Histidin (Contact Slide) General purpose media that supports the growth of nonfastidious as well as moderately fastidious microorganisms. Square contact slide, in sterile container 7.8 x 2.14 cm. 1 x Cellophane bagged. BD Dermatoslide BD Dermatoslide is a two-sided slide system for the detection and isolation of pathogenic fungi from superficial infections of skin, hair, and nails. It is used for primary isolation of fungi, especially dermatophytes, from a variety of superficial infections. It can also be used as a transport medium from the physician s office to analytical laboratories. BD Mycoslide Mycoslide is a set of dip slides for the detection of yeasts and fungi using 3 different agar media in urine specimens. Mycoslide is suitable for the primary cultivation of fungi and for pathogen count determinations of fungi. Suitable as a transport medium. Contains 3 slides, each with different agar media: Malt agar for determining the total pathogen count, Malt agar with cycloheximide for inhibition of fungi and some yeasts, and CLED agar for detection of bacterial infection or concomitant bacterial flora. BD Diagnostics Diagnostic Systems Catalog 2008/

96 Prepared Media BD Prepared Media for Environmental Monitoring BD Hycheck Contact Slides for Hygiene & Sterility Control Used to assess the microbiological contamination of surfaces or fluids. Double sided slides slides slides slides slides slides slides BD Hycheck - for Detection of Enterobacteriaceae Caseinpeptone soypeptone agar and crystal violet-neutralred-bile glucose agar. BD Hycheck - for Disinfection Control D/E Neutralizing agar on both sides of the slide. BD Hycheck - for Disinfection Control Caseinpeptone soypeptone agar and D/E Neutralizing agar. BD Hycheck - for Yeasts and Molds Tryptic soy agar and rose bengal chloramphenicol agar. BD Hycheck - for Yeasts and Molds with TTC Tryptic soy agar with 0.01 % TTC and rose bengal chloramphenicol agar. BD Hycheck - Plate Count Agar with TTC Plate count agar with TTC on both sides. BD Hycheck - Total Count Plate Count Agar and Plate Count Agar with TTC primarily for control of cutting oils, fuel oils and process water. Non-Gamma Irradiated Plates for Hygiene Monitoring BD RODAC and Contact plates are 60 mm-style plates used for surface sampling for microbial contamination. The base of the BD RODAC plate has inward-sloping walls and a convex bottom designed to keep the agar bed in place during transit and use plates plates plates plates plates plates plates plates plates plates BD RODAC - Contact Plate (Non-Sterile) D/E Neutralizing Agar - Contact Plates (65 mm) Nutrient Agar - Special Contact Plates Rose Bengal Agar with Chloramphenicol - Contact Plates (65 mm) Sabouraud Dextrose Agar with 2 % Dextrose - Contact Plates Sabouraud Glucose Agar with Chloramphenicol - Contact Plates BD Trypticase Soy Agar - Contact Plates 3 x 11 Contact plates sealed in cellophane bag. BD Trypticase Soy Agar with Lecithin and Polysorbate 80 - Contact Plates Packed in white cardboard boxes. Each sleeve of ten plates is wrapped into two cellophane bags and BD Flexopeel bag An indicator dot for gamma-irradiation is placed on the inner bag (orange to red= irradiated; yellow= not irradiated). Violet Red Bile Glucose Agar (VRBG Agar) - Contact Plates (65 mm) 3-34 BD Diagnostics Diagnostic Systems Catalog 2008/2009

97 Prepared Media BD Prepared Media for Environmental Monitoring Gamma Irradiated Plates for Hygiene Monitoring Available in many formulations and plate types including BD RODAC (Replicate Organism Detection and Counting) for personnel and surface sampling, Settling for active and passive air sampling, BD Finger Dab for sampling of gloved hands, and Contact plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates BD RODAC Unfilled Petri Dishes (Sterile, 65 mm) BD RODAC (Replicate Organism Detection and Counting) plates may be used for the detection and enumeration of microorganisms present on surfaces of sanitary importance. BD RODAC plates are recommended for use in a wide variety of surface sampling programs and may be employed to establish and monitor cleaning techniques and schedules. The 65 x 15 mm style dish is specially designed to allow a raised convex surface of culture medium. The 10 mm grid on the bottom of the plate facilitates counting and colony location. CDC Anaerobe Blood Agar Base Sterile Pack, BD RODAC Plates. CDC Anaerobe with Penicillinase Sterile Plates (100 mm). D/E Neutralizing Agar : 10 x Sterile Pack, BD RODAC Plates : 100 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack, Contact Plates : 100 x Sterile Plates (100 mm) : 10 x Sterile Plates (100 mm, 25 ml) D/E Neutralizing Agar with Penicillinase : 10 x Sterile Pack, BD RODAC Plates : 100 x Sterile Pack, BD RODAC Plates : 10 x Sterile Plates (100 mm) : 100 x Sterile Plates (100 mm) BD Microbial Content Test Agar (Gamma-Irradiated) Contact Dishes (65 mm). Rose Bengal Agar with Penicillinase : 10 x Sterile Plates (100 mm) : 10 x Sterile Plates, BD RODAC (100 mm) Sabouraud Dextrose Agar : 10 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack, Contact Plates : 10 x Sterile Plates (100 mm) : 5 x Sterile Plates (150 mm) : 10 x Sterile Plates, Triple Bagged (100 mm) Sabouraud Glucose Agar (Gamma-Irradiated) Sabouraud Dextrose Agar with Chloramphenicol Sterile Pack, BD RODAC Plates. Sabouraud Dextrose Agar with Lecithin and Polysorbate : 10 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack, Contact Plates BD Diagnostics Diagnostic Systems Catalog 2008/

98 Prepared Media BD Prepared Media for Environmental Monitoring Gamma Irradiated Plates for Hygiene Monitoring Available in many formulations and plate types including BD RODAC (Replicate Organism Detection and Counting) for personnel and surface sampling, Settling for active and passive air sampling, BD Finger Dab for sampling of gloved hands, and Contact plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates plates blue orange green BD Tryptic Soy Agar : 5 x Gamma-Irradiated 150 mm plates : 10 x Gamma-Irradiated plates, Triple Wrapped (Contact): 2 inner cellophane bags (inner one: with clip, label and irradiation indicator dot or stripe; outer one: closed with clip) Outer bag: Flexopeel bag, heat-sealed. BD Trypticase Soy Agar : 10 x Contact Plates : 100 x Gamma-Irradiated (90 mm) : 5 x Sterile Pack (150 mm) : 30 x Sterile Pack (150 mm) : 10 x Sterile Pack (90 mm) : 100 x Sterile Pack (90 mm) : 10 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack (Heavy Fill) : 100 x Sterile Pack (Heavy Fill) : 45 x Sterile Pack (Plates, 150 mm) : 5 x Sterile Pack, Settling Plates (150 mm) BD Trypticase Soy Agar with Lecithin and Polysorbate : 20 x Prepared Plates, BD RODAC : 100 x Prepared Plates, BD RODAC : 10 x Sterile Pack, BD Finger Dab Agar : 100 x Sterile Pack, BD Finger Dab Agar : 5 x Sterile Pack, BD Finger Dab Plates (150 mm) : 10 x Sterile Pack, BD RODAC Plates : 100 x Sterile Pack, BD RODAC Plates : 100 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack, Contact Plates : 100 x Sterile Pack, Contact Plates : 100 x Sterile Plates (100 mm) BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicillinase : 10 x Sterile Pack, BD RODAC Plates : 10 x Sterile Pack, Contact Plates BD Trypticase Soy Agar with Penicillinase : 10 x Sterile Pack, Settling Plates (100 mm) x Sterile Pack, Settling Plates (100 mm) : 5 x Sterile Pack, Settling Plates (150 mm) BD RODAC Plate-Rack 3-36 BD Diagnostics Diagnostic Systems Catalog 2008/2009

99 Prepared Media BD Prepared Media for Environmental Monitoring Gamma Irradiated Plates for Environmental Monitoring plates plates plates plates plates plates BD Microbial Content Test Agar Sterile Plates (90 mm). Packed in white cardboard boxes. Each sleeve of ten plates is wrapped into two cellophane bags and BD Flexopeel bag. An indicator dot for gamma-irradiation is placed on the inner bag (orange to red= irradiated; yellow= not irradiated). Sabouraud Glucose Agar with Chloramphenicol Sterile Pack, Prepared Plates (90 mm). BD Tryptic Soy Agar Gamma-Irradiated, Triple wrapped: 2 inner cellophane bags (inner one: welded and with irradiation indicator dot or stripe; outer one:closed with clip). Outer bag: BD Flexopeel bag, heat-sealed. BD Trypticase Soy Agar Sterile Pack, Triple bagged: one polyamide bag (containing one Ziplock bag), two Tyvek bags. BD Trypticase Soy Agar with Lecithin and Polysorbate 80 Prepared Plates, Double Bag. BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicinillase Sterile Pack. Packed in two Tyvek bags (with a Ziplock bag included in the inner Tyvek bag), followed by one white carton. BD Diagnostics Diagnostic Systems Catalog 2008/

100 Prepared Media BD Prepared Media for Environmental Monitoring Isolator Plates for Hygiene Monitoring Plated media in gamma-irradiated packs for isolator systems multi-wrap packaging protects media from vaporized hydrogen peroxide exposure. Available plate types for personnel and surface sampling and BD Finger Dab for sampling of gloved hands plates plates plates plates plates plates plates Brewer Anaerobic Agar with Lecithin and Polysorbate : Isolator Pack, Contact : Isolator Pack, Prepated Plates (100 mm) D/E Neutralizing Agar : 10 x Isolator Pack, BD Finger Dab Plates (100 mm) : 10 x Isolator Pack, BD RODAC Plates : 100 x Isolator Pack, BD RODAC Plates BD Microbial Content Test Agar : 10 x Isolator Pack; packed in one polyamide bag (containing one Ziplock bag), two Tyvek bags, followed by one white carton : 10 x Isolator Pack, Contact; packed in one polyamide bag (containing one Ziplock bag), two Tyvek bags, followed by one white carton plates plates plates plates plates Sabouraud Dextrose Agar with Lecithin, Polysorbate 80 and Chloramphenicol : 100 x Isolator Pack, Contact : 100 x Isolator Pack, Prepared Plates (100 mm) BD Trypticase Soy Agar : 10 x Isolator Pack, Triple Bagged : 100 x Isolator Pack, Triple Bagged: one polyamide bag (containing one Ziplock bag), two Tyvek bags : 5 x Isolator Pack, Prepared Plates (150 mm) plates plates plates plates plates plates plates plates BD Trypticase Soy Agar with Lecithin and Polysorbate : 100 x Isolator Pack, Contact Plates (100 mm) : 10 x Isolator Pack, BD Finger Dab Plates (100 mm) : 100 x Isolator Pack, BD Finger Dab Plates (100 mm) : 5 x Isolator Pack, BD Finger Dab Plates (150 mm) : 10 x Isolator Pack, BD RODAC Plates : 100 x Isolator Pack, BD RODAC Plates BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicillinase : 10 x Isolator Pack, Prepared Plates (100 mm) : 10 x Isolator Pack, BD RODAC Plates 3-38 BD Diagnostics Diagnostic Systems Catalog 2008/2009

101 Prepared Media BD Prepared Media for Environmental Monitoring BD Bottled Media for Sterility Testing Sterility Testing According to EP and USP x 100 ml x 100 ml x 100 ml x 300 ml x 400 ml x 650 ml x 300 ml x 650 ml x 1000 ml x 300 ml x 100 ml x 300 ml x 500 ml x 650 ml x 300 ml x 500 ml x 100 ml x 500 ml x 300 ml x 100 ml x 100 ml x 100 ml x 20 ml x 500 ml x 100 ml x 100 ml x 100 ml Fluid A : 25 x 100 ml Medium in 150 ml Laboratory Bottle with Metal Twist-Off Cap : 10 x 100 ml Medium in Prepared Bottle : 10 x 100 ml Medium in Prepared Septum Bottle : 10 x 300 ml Medium in 500 ml Infusion Bottle with Flip Tear-Off Seal and Stopper : 4 x 400 ml Medium in 500 ml Laboratory Bottle with Metal Twist-Off Cap : 4 x 650 ml Medium in Prepared Bottle Fluid A (Sterile Pack Bottle) : 10 x 300 ml Medium in Sterile Bottle with Septum Screw Cap : 4 x 650 ml Medium in 1000 ml Sterile Infusion Bottle with Septum : 4 x 1000 ml Medium in Sterile Prepared Bottle : 10 x 300 ml Medium in 500 ml Sterile Infusion Bottle with Septum Fluid D : 10 x 100 ml Medium in Prepared Serum Bottle : 10 x 300 ml Medium in Prepared Bottle with Septum Screw Cap : 10 x 500 ml Medium in Prepared Bottle Fluid D (Sterile Pack Bottle) : 4 x 650 ml Medium in 1000 ml Sterile Infusion Bottle with Septum : 10 x 300 ml Medium in 500 ml Sterile Infusion Bottle with Flip Tear-Off Seal and Stopper. Isopropylmyristate 500 ml Medium in Prepared Bottle. Peptone Water (0.1 %, DEP) 100 ml resp. 500 ml Medium in Prepared Bottle. Rinsing Fluid D 300 ml Medium in 500 ml Infusion Bottle with Flip Tear-Off Seal and Stopper Thioglycollate Medium, Fluid : 25 x 100 ml Medium in 150 ml Sirup Bottle with Screw Cap : 25 x 100 ml Medium in 100 ml Infusion Bottle, infusion stooper with tear ring x 100 ml Medium in 125 ml Sirup Bottle with Screw Cap : 50 x 20 ml Medium in 30 ml Screw Cap Vial : 10 x 500 ml Medium in 500 ml Infusion Bottle with Injection Stopper Thioglycollate Medium, Fluid (Double Strength) 100 ml Medium in 250 ml Infusion Bottle with Injection Stopper. Thioglycollate Medium, Fluid, Modified 100 ml Medium in 150 ml Sirup Bottle with Screw Cap and injection hole. Thioglycollate Medium, Fluid with 1 % Tween 100 ml Medium in 150 ml Sirup Bottle with Screw Cap. BD Diagnostics Diagnostic Systems Catalog 2008/

102 Prepared Media BD Prepared Media for Environmental Monitoring BD Bottled Media for Sterility Testing Sterility Testing According to EP and USP x 100 ml x 100 ml x 125 ml x 100 ml x 100 ml x 100 ml x 500 ml x 100 ml x 100 ml x 800 ml x 100 ml x 20 ml x 9 ml x 500 ml x 100 ml x 100 ml x 100 ml x 100 ml x 100 ml x 100 ml x 100 ml x 20 ml x 50 ml x 50 ml x 500 ml x 90 ml x 3 l x 5 l l Thioglycollate Medium, Fluid (FTM Special) Special Quality = improved medium with a higher clarity : 25 x 100 ml Medium in 125 ml Sirup Bottle with Screw Cap : 25 x 100 ml Medium in 150 ml Sirup Bottle with Screw Cap : 25 x 125 ml Medium in 125 ml Infusion Bottle with stopper and flip-tear-off cap. Thioglycollate Medium, Fluid (FTM Special, ETO sterile) : 48 x 100 ml Medium in 100 ml Veral Glass Bottle with Screw Cap and integrated septum : 44 x 100 ml Medium in 125 ml Infusion bottle with infusion stopper, with tear ring Thioglycollate Medium, Fluid (FTM Special, Sterile Pack Bottle) : 10 x 100 ml Medium in 125 ml Sterile Infusion Bottle with stopper & flip-tear-off cap : 10 x 500 ml Medium in 500 ml Sterile Laboratory Bottle with Screw Cap : 10 x 100 ml Medium in 125 ml Sterile Infusion Bottle with injection stopper : 10 x 100 ml Medium in 8 oz Sterile Wide Mouth Jar with Twist-off cap : 4 x 800 ml Medium in 1000 ml Sterile Infusion Bottle with infusion stopper BD Tryptic Soy Broth : 10 x 100 ml Medium in Prepared Wide Mouth Bottle with Screw Cap : 50 x 20 ml Medium in 30 ml Screw Cap Vial : 50 x 9 ml Medium in 30 ml Screw Cap Vial BD Trypticase Soy Agar 500 ml Medium in Prepared Bottle with Screw Cap. BD Trypticase Soy Broth : 25 x 100 ml Medium in 125 ml Infusion Bottle with Septum : 25 x 100 ml Medium in 125 ml Sirup Bottle with Screw Cap : 25 x 100 ml Medium in 150 ml Sirup Bottle with Screw Cap and integrated Septum : 25 x 100 ml Medium in 150 ml Sirup Bottle with Screw Cap : 10 x 100 ml Medium in 210 ml Wide Mouth Jar with Twist-Off Cap : 10 x 100 ml Medium in 380 ml Jar with Metall Twist-Off Cap : 10 x 100 ml Medium in Prepared Bottle with Septum Screw Cap : 10 x 20 ml Medium in 500 ml Infusion Bottle with crimp cap and injection stopper : 25 x 50 ml Medium in 125 ml Sirup Bottle with Screw Cap : 25 x 50 ml Medium in 90 ml Sirup Bottle with Screw Cap and integrated Septum : 10 x 500 ml Medium in Prepared Bottle with Septum Screw Cap : 10 x 90 ml Medium in 250 ml Infusion Bottle with Septum BD Trypticase Soy Broth (Serum Bags) Serum Bags: 3, 5 and 10 l BD Diagnostics Diagnostic Systems Catalog 2008/2009

103 Prepared Media BD Prepared Media for Environmental Monitoring BD Bottled Media for Sterility Testing Sterility Testing According to EP and USP x 100 ml x 100 ml x 50 ml x 50 ml x 500 ml x 50 ml x 100 ml x 100 ml x 250 ml x 800 ml x 100 ml x 500 ml x 800 ml x 100 ml BD Trypticase Soy Broth (Double Strength) : 25 x 100 ml Medium in 100 ml Infusion Bottle with Septum : 10 x 100 ml Medium in 250 ml Infusion Bottle with Setpum : 25 x 50 ml Medium in 100 ml Infusion Bottle with Septum : 25 x 50 ml Medium in 150 ml Jar with Metall Twist-Off Cap : 10 x 500 ml Medium in 500 ml Infusion Bottle with Septum BD Trypticase Soy Broth (ETO sterile) 50 ml Medium in 100 ml Glass Bottle with Screw Cap and integrated Septum, Sterile. BD Trypticase Soy Broth, Modified 100 ml Medium in 150 ml Sirup Bottle with Screw Cap and injection hole. BD Trypticase Soy Broth (Sterile Pack Bottle) : 10 x 100 ml Medium in 125 ml Sterile Infusion Bottle with Infusion Stopper & pull ring : 10 x 150 ml Medium in 380 ml Sterile Wide Mouth Bottle with Twist-Off Cap : 4 x 800 ml Medium in 1000 ml Sterile Sirup Bottle with Aluminium Screw Cap : 10 x 100 ml Medium in 125 ml Sterile Infusion Bottle with Septum : 10 x 500 ml Medium in 500 ml Sterile Infusion Bottle with Septum : 4 x 800 ml Medium in Sterile Prepared Bottle BD Trypticase Soy Broth with 1 % Tween 100 ml Medium in 150 ml Sirup Bottle with Screw Cap and integrated Septum. BD Diagnostics Diagnostic Systems Catalog 2008/

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105 Environmental Monitoring Systems In the late 1960s, BD launched the first ever commercial system for environmental generation, eliminating the need for vacuum pumps, gas tanks, nanometers and release valves. Over the next 40 plus years, BD has introduced a series of continuallyinnovative and high performance products into this market. For the generation of anaerobic, micro-aerophilic or C0 2 -enriched atmospheres in containers or jars, BD offers the BD GasPak EZ Container System. For the generation of anaerobic, microaerophilic or C0 2 -enriched atmospheres in pouches BD offers the BD GasPak EZ Pouch. Indicators may be placed in the vesicle to indicate when anaerobiasis or C0 2 generation is complete. BD GasPak TM EZ s 4-2 BD GasPak TM 100 & 150 System 4-3 Environmental Chamber 4-6

106 Environmental Systems BD GasPak EZ s BD GasPak EZ Container Systems BD GasPak EZ Container Systems offer waterless, catalyst-free convenience for use in producing anaerobic, microaerophilic, or CO2-enriched environments sachets sachets sachets plates plates BD GasPak EZ - Anaerobe Container System Sachets BD GasPak EZ Container System Sachets are sold in packages of 20 sachets and are available for the generation of anaerobic, microaerophilic, or CO2 enriched environments. The sachets are activated immediately upon opening of the outer foil packaging and do not require the addition of water or catalyst. Contains 20 anaerobe container sachets. BD GasPak EZ - Campy Container System Sachets For the generation of microaerophilic environments. BD GasPak EZ - CO2 Container System Sachets For the generation of CO2 enriched environments. BD GasPak EZ - Incubation Container BD GasPak EZ Container Systems offer waterless, catalyst-free convenience for use in producing anaerobic, microaerophilic, or CO2-enriched environments. Available in two convenient sizes, BD GasPak Incubation Containers are constructed of nonbreakable, chemical resistant material designed to maintain a desired environment throughout incubation : Standard Incubation Container; holds up to 15/18 Petri dishes : Large Incubation Container; holds up to 30/33 dishes rack rack BD GasPak EZ - Container Rack Removable BD GasPak EZ Container Racks are available to secure Petri dishes and ease workflow : The STANDARD Rack is designed to hold up to 18 Petri dishes : The LARGE Rack is designed to hold up to 33 dishes sachets BD GasPak EZ - Sachet Anaerobe with Indicator GasPak EZ Anaerobe be Container System Sachets with Indicator 20 Sachets Per Box 4-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

107 Environmental Systems BD GasPak EZ s BD GasPak EZ Pouch Systems pouches BD GasPak EZ - Anaerobe Pouch System BD GasPak EZ Pouch Systems offer the convenience of pouches integrated into a complete kit with everything you need to generate a pouch based anaerobic, microaerophilic, or CO2-enriched environment. Anaerobic indicators are provided with the BD GasPak EZ Anaerobic Pouch System. The BD GasPak EZ Pouch Systems feature one-step resealable pouches, which have been specially designed to maximize the preservation of the desired environment throughout incubation. The System is also waterless and catalyst free, it contains: 20 sachets, 20 resealable pouches & 20 Dry Anaerobic Indicators) pouches pouches BD GasPak EZ - BD CampyPouch System For the generation of microaerophilic environments. Contains 20 sachets and 20 resealable pouches. BD GasPak EZ - CO2 Pouch System For the generation of CO2 enriched environments. Containing 20 sachets and 20 resealable pouches. BD Diagnostics Diagnostic Systems Catalog 2008/

108 Environmental Systems BD GasPak and BD GasPak 100 & 150 System BD GasPak Disposable Indicators strips strips BD GasPak - CO2 Indicator Strips CO2 Indicator Reagent Droppers: Indicates when an ideal carbon dioxide-enriched environment has been achieved. BD GasPak - Dry Anaerobic Indicator Strips Contains a dry indicator pad that changes from blue to colorless in the absence of oxygen. Gas Generator Envelopes for BD GasPak 100 & BD CampyPak Plus - Disposable Hydrogen + Carbon Dioxide Generator Envelope BD CampyPak Plus is a system for generating a reduced oxygen, enriched carbon dioxide environment within BD GasPak jars. When properly activated and incubated, the envelope will rapidly produce conditions conducive to the primary isolation and cultivation of microaerophilic organisms, such as Campylobacter jejuni, C. coli and C. fetus subsp. fetus. The actual atmosphere in the BD GasPak system will vary according to the number of inoculated plates and on the elevation of the laboratory above sea level. Contains: BD CampyPak Plus Microaerophilic System Envelopes with Palladium Catalyst, Disposable H2 + CO2 Generator Envelopes, Pkg. of 10 envelopes. Each BD CampyPak Plus with Palladium Catalyst disposable hydrogen + carbon dioxide generator envelope consists of: One Sodium Borohydride Tablet, One Sodium Bicarbonate Plus Citric Acid Tablet, One Filter Paper (piece), Palladium catalyst Sachet. BD GasPak Plus - Bulk Kit BD GasPak Plus is an improved disposable hydrogen and carbon dioxide generating envelope with catalyst sachet that produces an atmosphere in BD GasPak jars suitable to support the primary isolation and cultivation of anaerobic bacteria. Contains: BD GasPak Plus Anaerobic System Envelopes with Palladium Catalyst, Disposable H2 + CO2 Generator Envelopes, Pkg. of 100 envelopes (100 BD GasPak Dry Anaerobic Indicators also provided). Each BD GasPak disposable hydrogen + carbon dioxide generator envelope consists of: One Sodium Borohydride Tablet, One Sodium Bicarbonate Plus Citric Acid Tablet, One Filter Paper (piece), Palladium Catalyst Sachet. BD GasPak Plus - Disposable Hydrogen + Carbon Dioxide Generator Envelope BD GasPak Plus is an improved disposable hydrogen and carbon dioxide generating envelope with catalyst sachet that produces an atmosphere in BD GasPak jars suitable to support the primary isolation and cultivation of anaerobic bacteria. Contains: BD GasPak Plus Anaerobic System Envelopes with Palladium Catalyst, Disposable H2 + CO2 Generator Envelopes, Pkg. of 10 envelopes. Each BD GasPak disposable hydrogen + carbon dioxide generator envelope consists of: One Sodium Borohydride Tablet, One Sodium Bicarbonate Plus Citric Acid Tablet, One Filter Paper (piece), Palladium Catalyst Sachet. 4-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

109 Environmental Systems BD GasPak and BD GasPak 100 & 150 System BD GasPak 100 & 150 Equipment BD GasPak Holding Jar Complete with one jar and one holding jar lid. BD GasPak Holding Jar Lid Complete with "O" ring, improved clamp/thumbscrew assembly, and one piece of tubing. BD GasPak Lid With improved clamp/thumbscrew assembly, "0" ring gasket, and catalyst reaction chamber, with two catalyst charges. BD GasPak Lid without O-Ring Without clamp screw, catalyst reaction chamber or catalyst charges. BD GasPak Polycarbonate Jar without Lid BD GasPak System Complete Consists of polycarbonate jar, lid with ''O'' ring gasket, improved clamp/thumbscrew assembly and catalyst reaction chamber, two catalyst charges, one rack and one tube holder. BD GasPak System Complete (Vented) Similar to , except that a vented lid is substituted for the nonvented lid. BD GasPak Vented Lid (Complete) Complete with improved clamp/thumbscrew assembly, "O'' ring gasket, catalyst reaction chamber, two catalyst charges, rubber tube and tubing clamp. BD GasPak Inner Lid Without outer lid and thumbscrew, catalyst reaction chambers or catalyst charges. BD GasPak Anaerobic Jar without Lid (Large) BD GasPak Anaerobic System Complete (Large) Consists of polycarbonate jar (nonvented), lid assembly with "O" ring gasket and catalyst reaction chambers (3 each), ten catalyst charges, one rack and one tube holder. BD GasPak Anaerobic System Complete (Vented, Large) Similar to except that the jar is supplied with the vent installed, rubber tube and tubing clamp. BD GasPak Lid Assembly Consists of outer lid and thumb screw, inner lid, large "O" ring gasket, three catalyst reaction chambers and six catalyst charges. BD Diagnostics Diagnostic Systems Catalog 2008/

110 Environmental Systems BD GasPak and BD GasPak 100 & 150 System BD GasPak 100 & 150 Replacement Parts BD GasPak Improved Clamp/Thumbscrew Assembly BD GasPak "O" Ring Gasket For BD GasPak 100 Lid. BD GasPak Rack Holds 36 Petri dishes (100 x 15 mm). BD GasPak 100 and Tube Holder Holds up to 13 tubes (16,5 x 128 mm). 4-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

111 Environmental Systems Environmental Chamber BD Bio-Bag BD Bio-Bag Type C BD Bio-Bag Type C is a disposable environmental system designed to rapidly generate and maintain a carbon dioxide environment for 48 h. Each system contains a sealable translucent BD Bio-Bag and a CO2 Gas Generator. A CO2-enriched atmosphere is conducive to improved recovery of certain microorganisms; i.e., Neisseria gonorrhoeae,1,2 Neisseria meningitidis and Haemophilus species. Growth and hemolysis on blood agar of some strains of streptococci are enhanced by 5 to 10% CO2. BD Bio- Bag Type C is designed to generate and maintain a CO2-fortified atmosphere for at least 48 h. This disposable system is intended to be used with appropriate media provided by the user. After incubation, bacterial growth may be macroscopically observed through the Bio-Bag at any interval without disrupting the CO2-enriched atmosphere. Each BD Bio-Bag Type C system consists of: 1 Gas Impermeable Environmental Chamber with a zipper closure. 1 Gas Generator consisting of one tablet of 130 mg sodium bicarbonate and one ampule of 0.5 ml of 1.56 N hydrochloric acid. 50 sets per carton / 50 generators. BD Diagnostics Diagnostic Systems Catalog 2008/

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113 Direct Testing Systems & Serology With more than two decades of extensive research and production experience, BD Diagnostic Systems has exhibited strong leadership in rapid detection of a wide range of pathogens including RSV and influenza virus, C. difficile, Giardia Cryptosporidium and Group A Strep. When it comes to serology testing, BD latex agglutination tests and RPR card tests have come to be the industry standards by which other tests are measured. BD serology testing products are stand out performers for accuracy, sensitivity and convenience. The broad product offerings and high quality are well known and trusted by laboratories everywhere. BD Rapid Manual Tests 5-2 BD Serology Diagnostics 5-10

114 Direct Testing Systems & Serology BD Rapid Manual Tests BD LINK2 Helicobacter pylori Tests tests tests BD LINK2 - H. Pylori Rapid Test Kit A qualitative immunochemical membrane assay for the detection of lgg antibodies to H. pylori in whole blood by visual interpretation. The test is for professional use as an aid in the diagnosis of H. pylori infection in adult symptomatic patients. BD LINK2 - H. Pylori Rapid Test Quality Control Kit The BD LINK2 H. pylori Rapid Test Quality Control Kit, available separately, is designed to be used in conjunction with the BD LINK2 H. pylori Rapid Test to verify test performance. 5-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

115 Direct Testing Systems & Serology BD Rapid Manual Tests BD Directigen Test Systems tests BD Directigen - H. influenzae type B Test Kit BD Directigen H. influenzae type B test kit is a presumptive latex agglutination test for the direct qualitative detection of antigens to H. influenza type B. 30 patient tests, 60 controls - 90 determinations containing: one 1 ml bottle H. influenzae type b antibody, Reagent 1 one 1.5 ml bottle Positive Control one 1.5 ml bottle Negative Control one 0.5 ml bottle control latex Reagent A stirrers, plastic tests BD Directigen - Meningitis Combo Test Kit BD Directigen Meningitis Combo Test is a presumptive latex agglutination test for the direct qualitative detection of antigens to H. influenzae type b, S. pneumoniae, Group B Streptococcus, N. meningitidis Groups A, B, C, Y or W135, and Escherichia coli K1 in cerebrospinal fluid (CSF), serum, urine or blood culture media. In addition, the test kit provides confirmation and serogrouping capabilities from suspected colonies of H. influenzae type b, S. pneumoniae, Group B Streptococcus, and N. meningitidis Groups A/Y, B or C/ W135. Visible agglutination occurs when a sample containing any of these bacterial antigens reacts with the respective antibodycoated latex beads. 30 patient tests, 60 controls - 90 determinations containing: one 9 ml bottle Positive Control one 9 ml bottle Negative Control one 1 ml bottle H. influenzae type b antibody, Reagent 1 one 1 ml bottle S. pneumoniae antibody, Reagent 2 one 1 ml bottle Group B Streptococcus antibody, Reagent 3 one 1 ml bottle N. meningitidis Groups C and W135 antibody, Reagent 4 one 1 ml bottle N. meningitidis Groups A and Y antibody, Reagent 5 one 1 ml bottle N. meningitidis Group B/E. coli K1 antibody, Reagent 6 one 8 ml bottle Specimen B one 0.5 ml bottle control latex Reagent A (rabbit) one 0.5 ml bottle control latex Reagent B (mouse) 30 single use test cards plastic stirrers, workstation and procedure chart cards ml tests BD Directigen - Meningitis Combo Test Cards Contains 30 single use test cards for use with the BD Directigen Meningitis Combo Test Kit ( ). BD Directigen - Meningitis Specimen Buffer 8 ml bottle solution containing EDTA for use with Cat. Nos , , , , and Included in each Combo Test Kit; also available separately. BD Directigen - N. meningitidis Group B/E. coli K1 Test Kit BD Directigen N. meningitidis Group B/E. coli K1 Test Kit is a presumptive latex agglutination test for the direct qualitative detection of antigens to N. meningitidis Group B and E. coli K1. 30 patient tests, 60 controls - 90 determinations containing: one 1 ml bottle N. meningitidis Group B/E. coli K1 antibody, Reagent 1 one 1.5 ml bottle Positive Control one 1.5 ml bottle Negative Control one 0.5 ml bottle control latex Reagent A stirrers, plastic BD Diagnostics Diagnostic Systems Catalog 2008/

116 Direct Testing Systems & Serology BD Rapid Manual Tests BD Directigen Test Systems tests BD Directigen - N. meningitidis Groups A, C, Y and W135 Test Kit This test kit Test is a presumptive latex slide agglutination test for the qualitative detection of antigens to Neisseria meningitidis Groups A, C, Y and W135 directly in cerebrospinal fluid (CSF), serum or urine. In addition, the test kit provides confirmation and serogrouping capabilities from suspected colonies of N. meningitidis Groups A/Y or C/W135. Visible agglutination occurs when a sample containing any of these bacterial antigens is reacted with its respective antibody-coated latex beads. 30 patient tests, 60 controls - 90 determinations containing: one 1 ml bottle N. meningitidis Groups C and W135 antibody, Reagent 1 one 1 ml bottle N. meningitidis Groups A and Y antibody, Reagent 2 one 3 ml bottle Positive Control one 3 ml bottle Negative Control one 0.5 ml bottle control latex Reagent A stirrers, plastic tests BD Directigen - S. pneumoniae Test Kit BD Directigen S. pneumoniae test kit is a presumptive latex agglutination test for the direct qualitative detection of S. pneumoniae. 30 patient tests, 60 controls - 90 determinations containing: one 1 ml bottle S. pneumoniae antibody, Reagent 1 one 1.5 ml bottle Positive Control one 1.5 ml bottle Negative Control one 0.5 ml bottle control latex Reagent A stirrers, plastic BD GonoGen II for the Confirmation of Neisseria gonorrhoeae tests BD GonoGen II Monoclonal antibody-based colorimetric test intended for the confirmatory identification of Neisseria gonorrhoeae from culture. The GonoGen II test for N. gonorrhoeae contains a specific anti-gonococcal reagent composed of a pool of murine monoclonal antibodies (IgG) that have been prepared against a purified outer membrane protein, Protein I of N. gonorrhoeae. Contains reagents for 24 tests. BD Staphyloslide Staphylococcus aureus Tests tests BD Staphyloslide - Latex Test Kit The BD Staphyloslide Latex Test is a latex slide agglutination test for the differentiation of staphylococci which possess clumping factor and/or Protein A, usually present with Staphylococcus aureus, from staphylococci that do not possess these properties. 5-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

117 Direct Testing Systems & Serology BD Rapid Manual Tests BD Streptococcus Tests determ. BD Directigen - Group B Strep Test Kit BD Directigen Group B Strep Test Kit is a presumptive latex agglutination test for the direct qualitative detection of Group B streptococci. 30 patient tests, 60 controls - 90 determinations containing: one 1 ml bottle Group B Strep antibody, Reagent 1 one 1.5 ml bottle Positive Control one 1.5 ml bottle Negative Control one 0.5 ml bottle control latex Reagent A stirrers, plastic tests tests tests ml ml ml ml g ml cards BD Pneumoslide - Test for Streptococcus pneumoniae Consists of one 2.5 ml vial with dropper of BD Pneumoslide S. pneumoniae Antibody-Coated Latex Bead Suspension, one vial, Lyophilized (restores to 0.5 ml), BD Pneumoslide Polyvalent Positive Control, one 2.5 ml vial with dropper, BD Pneumoslide Latex Negative Control, 50 Stirring Sticks and one Glass Slide. This product is not CE marked. BD Streptocard - Enzyme Latex Test Kit Used for identification of Lancefield streptococcal groups A, B, C, F and G from streptococcus colonies that are ß-hemolytic and non-hemolytic. The BD Streptocard Enzyme Latex Test latex particles are sensitized with group specific antibody and will agglutinate in the presence of homologous antigen. In the absence of such antigen, the latex particles will remain in a smooth suspension. The use of a patented enzymatic extraction in the BD Streptocard Enzyme Latex Test procedure considerably shortens the time required for antigen extraction and improves the antigen yield. Test Latex A, B, C, D, F and G consist of blue latex particles sensitized with rabbit antibody to appropriate group specific antigen, suspended in buffer containing 0.1 % sodium azide (preservative). Contains reagents for 50 tests. BD Streptocard - Acid Latex Test Kit Used for identification of Lancefield streptococcal groups A, B, C, F and G from streptococcus colonies that are ß-hemolytic. Contains reagents for 50 tests. BD Streptocard - Group A Latex Reagent BD Streptocard - Group B Latex Reagent BD Streptocard - Group D Latex Reagent BD Streptocard - Acid Extraction Reagent BD Streptocard - Enzyme Extraction Reagent 1 x 2.1 g Bottle. BD Streptocard - Positive Control BD Streptocard - Reaction Cards BD Diagnostics Diagnostic Systems Catalog 2008/

118 Direct Testing Systems & Serology BD Rapid Manual Tests BD Stool Diagnostics tests BD ColorPAC - Toxin A Test Kit BD ColorPAC Toxin A Test is a rapid chromatographic assay for the qualitative detection of Clostridium difficile toxin A (enterotoxin) in stool specimens from patients suspected of having C. difficile-associated disease. The test can also be used for confirmation of suspect colonies of toxigenic C. difficile from agar plates or BHI broth. This assay is intended for use as an aid in the diagnosis of C. difficile-associated disease. (30 tests) C. difficile Toxin A Test containing: one bottle Sample Buffer, Reagent 1 one bottle Detector A, Reagent 2 one bottle Detector B, Reagent 3 one bottle Wash Reagent, Reagent W one bottle Positive Control Control+ one bottle Negative Control Control- 30 Test Devices 30 SQ-EASY tubes and fiber tips each 30 applicator sticks and transfer pipets 1 dropper rod 5-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

119 Direct Testing Systems & Serology BD Rapid Manual Tests BD Directigen Viral Diagnsotic Testing Systems tests BD Directigen - Flu A+B Test Kit BD Directigen Flu A+B test is a rapid in vitro immunoassay membrane test for the direct and qualitative detection of influenza A and B viral antigens from nasopharyngeal wash, nasopharyngeal aspirate, nasopharyngeal swab, lower nasal swab, throat swab and bronchoalveolar lavage specimens of symptomatic patients. The BD Directigen Flu A+B test is a differentiated test, and therefore influenza A viral antigens can be distinguished from influenza B viral antigens in a single test. The test is to be used as an aid in the diagnosis of influenza A and B viral infections. Negative test results should be confirmed by cell culture. The BD Directigen Flu A+B test is not intended for detection of influenza C. (20 tests) containing: one 9.9 ml bottle Extraction Reagent E one 5.1 ml bottle Wash Reagent 1 one 2.3 ml Detection Reagent 2 one 2.3 ml bottle Detection Reagent 3 one 6.5 ml bottle Wash Reagent 4 one 10.5 ml bottle Wash Reagent 5 one 5.8 Substrate Reagent 6 one 4.1 ml bottle Stop Reagent 7 one 2.0 ml bottle Control A+/B one 2.0 ml bottle Control B+/A each one bag of 20 BD DispensTube Tubes and 20 BD DispensTube Tips one 20 pack BD ColorPac Devices Tests BD Directigen EZ - Flu A+B For the Differentiated, Direct Detection of Influenza A and B Viral Antigens. The BD Directigen EZ - Flu A+B test is a rapid chromatographic immunoassay for the direct and qualitative detection of influenza A and B viral antigens from nasopharyngeal washes/aspirates and throat swabs of symptomatic patients. The BD Directigen EZ - Flu A+B test is a differentiated test, such that influenza A viral antigens can be distinguished from influenza B viral antigens from a single processed sample using a single device. The test is to be used as an aid in the diagnosis of influenza A and B viral infections. All negative test results should be confirmed by cell culture. The BD Directigen EZ - Flu A+B test is a chromatographic immunoassay to detect influenza A or B antigens from respiratory specimens of symptomatic patients with time to results of 15 min. The following components are included in the BD Directigen EZ Flu A+B kit: 30 x Directigen EZ - Flu A+B Devices one 4.7 ml bottle Reagent E one 2.8 ml bottle Control A+/B- one 2.8 ml bottle Control B+/A- 30 x BD DispensTube Tubes 30 x BD DispensTube Tips BD Diagnostics Diagnostic Systems Catalog 2008/

120 Direct Testing Systems & Serology BD Rapid Manual Tests BD Directigen Viral Diagnsotic Testing Systems tests BD Directigen - Flu A Test Kit The BD Directigen Flu A Test is an in vitro immunoassay membrane test for the direct rapid and qualitative detection of influenza A viral antigen from suitable specimens of symptomatic patients. Nasopharyngeal wash and aspirate specimens have been shown to be superior to nasopharyngeal and throat swab specimens and are the specimens of choice with the BD Directigen Flu A Test. (20 tests) containing: one 5.0 ml bottle Extraction Reagent A one 7.5 ml bottle Wash Reagent 1 one 4.7 ml bottle Wash Reagent 2 one 4.3 ml bottle Detector Reagent 3 one 7.5 ml bottle Wash Reagent 4 one 7.3 ml bottle Wash Reagent 5 one 4.5 ml bottle Substrate A, Reagent 6 one 3.9 ml bottle Substrate B, Reagent 7 one 4.1 ml bottle Stop Reagent 7 each one 2.0 ml bottle Positive Control and Negative Control 20 BD ColorPAC devices with flow control units BD DispensTube devices tests BD Directigen - RSV Test Kit In vitro enzyme immunoassay (EIA) membrane test for the rapid and qualitative detection of RSV (Respiratory Syncytial Virus) antigen directly from nasopharyngeal specimens. (20 tests) containing: one 4.5 ml bottle Extraction Reagent A one 7 ml bottle Wash Reagent 1 one 3.5 ml bottle Detector Reagent 2 one 13.5 ml bottle Wash Reagent 3 one 7 ml bottle Substrate A, Reagent 4 one 7 ml bottle Substrate B, Reagent 5 one 7 ml bottle Stop Reagent 6 one 1 ml bottle Positive Control one 1 ml bottle Negative Control 20 BD ColorPAC devices with flow control units BD DispensTube devices tests BD Directigen EZ - RSV Test Kit The BD Directigen EZ RSV test is a rapid chromatographic immunoassay for the direct and qualitative detection of Respiratory Syncytial Virus (RSV) antigen in nasopharyngeal washes, nasopharyngeal aspirates, nasopharyngeal swabs and nasopharyngeal swab/washes from patients suspected of having a viral respiratory infection. This test is intended for in vitro diagnostic use to aid in the diagnosis of Respiratory Syncytial Virus (RSV) infections in neonatal and pediatric patients under the age of 20. It is recommended that negative test results be confirmed by cell culture. (30 tests ) containing: one 4.5 ml bottle Extraction Reagent E one 2.0 ml bottle Positive Control one 2.0 ml bottle Negative Control 30 BD Directigen EZ RSV Devices each 30 BD DispensTube Tubes and BD DispensTube Tips 5-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

121 Direct Testing Systems & Serology BD Rapid Manual Tests BD Directigen Viral Diagnsotic Testing Systems tests BD Directigen - RSV Test Kit In vitro enzyme immunoassay (EIA) membrane test for the rapid and qualitative detection of RSV (Respiratory Syncytial Virus) antigen directly from nasopharyngeal specimens. (40 tests) containing: one 4.5 ml bottle Extraction Reagent A one 7 ml bottle Wash Reagent 1 one 7 ml bottle Detector Reagent 2 one 13.5 ml bottle Wash Reagent 3 one 7 ml bottle Substrate A, Reagent 4 one 7 ml bottle Substrate B, Reagent 5 one 7 ml bottle Stop Reagent 6 one 1 ml bottle Positive Control one 1 ml bottle Negative Control 40 BD ColorPAC devices with flow control units BD DispensTube devices BD Diagnostics Diagnostic Systems Catalog 2008/

122 Direct Testing Systems & Serology BD Serology Diagnostics BD Macro-Vue RPR Kits tests BD Macro-Vue RPR - Card Test Kit No. 104 Nontreponemal testing procedure for the serologic detection of syphilis. (300 qualitative tests) containing: 2 x 3 ml ampules antigen 20 G dispensing needle antigen dispensing bottle 350 stirrers 30 cards with 10 x 18 mm circle spots each 300 x 0.05 ml capillaries tests BD Macro-Vue RPR - Card Test Kit No. 110 Nontreponemal testing procedure for the serologic detection of syphilis. (500 qualitative tests) containing: 3 x 3 ml ampules antigen 20 G dispensing needle antigen dispensing bottle 50 cards with 10 x 18 mm circle spots each 500 BD Dispenstirs devices, 0.05 ml delivery tests BD Macro-Vue RPR - Card Test Kit No. 112 Nontreponemal testing procedure for the serologic detection of syphilis. (150 quantitative tests) containing: 5 x 3 ml ampules antigen 20 G dispensing needle antigen dispensing bottle 200 stirrers 50 cards (quantitative) with 15 x 18 mm circle spots each 150 x 0.05 ml capillaries tests BD Macro-Vue RPR - Card Test Kit No. 115 Nontreponemal testing procedure for the serologic detection of syphilis. (150 qualitative tests) containing: 1 x 3 ml ampules antigen 20 G dispensing needle antigen dispensing bottle 15 cards with 10 x 18 mm circle spots each 150 BD Dispenstirs Devices, 0.05 ml delivery 5-10 BD Diagnostics Diagnostic Systems Catalog 2008/2009

123 Direct Testing Systems & Serology BD Serology Diagnostics BD Macro-Vue RPR Controls cards x 1.5 ml BD Macro-Vue RPR - Control Cards BD Macro-Vue RPR Card Test Control Cards are dehydrated control specimens of predetermined reactivity for quality-control testing of antigen before use in performing the BD Macro-Vue RPR Card Tests for the serologic detection of syphilis. The cards were designed for routine, daily use as check-test specimens to determine the acceptable reactivity of RPR Card antigen suspension. RPR Card antigen is a cardiolipin type antigen, which detects "reagin", an antibody-like substance present in serums from syphilitic persons, and occasionally in serums of persons with other acute and chronic conditions. The RPR 18 mm Circle Card Test Control Card consists of three circles designated as: Reactive, Reactive minimal-to-moderate and Nonreactive. The labelled areas on this card contain graded reactivity specimens. The specimen material is human plasma purchased from licensed blood banks as Reactive or Nonreactive for syphilis. BD Macro-Vue RPR - Liquid Controls BD Macro-Vue RPR (Rapid Plasma Reagin) Card Test Liquid Controls are designed as an unassayed control material to monitor, at three reaction levels, the precision of BD Macro-Vue RPR 18 mm Circle Card Test. RPR Test Reagents should be routinely tested for patterns of graded reactivity against controls with established patterns of reactivity. Contains pooled human serum with 0.1 % sodium azide as a preservative. Control ++, 1.5 ml BD Macro-Vue RPR Card Test Reactive Control Serum Positive. Control +, 1.5 ml BD Macro-Vue RPR Card Test Moderately Reactive Control Serum Weakly Positive. Control -, 1.5 ml BD Macro-Vue RPR Card Test Negative Control Serum Negative Control. BD Diagnostics Diagnostic Systems Catalog 2008/

124 Direct Testing Systems & Serology BD Serology Diagnostics BD Macro-Vue RPR Components x 3 ml x 3 ml BD Macro-Vue RPR - Antigen (Plastic) Dispensing Bottle BD Macro-Vue RPR Card - Antigen Suspension These cards are a component of the BD Macro-Vue RPR Card Tests (Qualitative and Quantitative) which is for the serologic detection of syphilis. RPR Card Antigen Suspension is a reagent component part used in performing the BD Macro-Vue RPR (Rapid Plasma Reagin) 18 mm Circle and Teardrop Card Tests which are nontreponemal testing procedures for the serologic detection of syphilis and are described in "A Manual of Tests for Syphilis". The test techniques described in this manual are the most widely used in the United States and have been thoroughly evaluated and standardized. Suspension (dated and refrigerated product): : 3 ml ampule, for use with the 18 mm Circle Card Test, Box of three : 3 ml ampule, for use with the 18 mm Circle Card Test, Box of ten x x x BD Macro-Vue RPR Card - Brewer Diagnostic For qualitative determination with 10, 15 and resp. 30 spot cards of 18 mm circle. BD Macro-Vue RPR Card - Test Rotator Accessories Package Package contains: One 7 x 15'' rotator extension top, two humidifying covers. BD Macro-Vue RPR Card - Test Rotator Model 51 Constant 100 rpm, 2 cm diameter, automatically controlled timer, one humidifying cover. BD Macro-Vue RPR Needle (Dispensing) 20 G Needles, Dispensing (square cut), yellow hub. BD Dispenstirs Device Single use, plastic pipettes, 0.05 ml volume (for use with 18 mm circle RPR card tests). BD Syphilis Reagents ml x 0.5 ml kit VDRL Cardiolpin Antigen With 60 ml VDRL buffered saline. This reagent is used in the qualitative and quantitative determination in the following nontreponemal serologic tests for syphilis: VDRL Slide Qualitative Test with Serum, VDRL Slide Quantitative Test with Serum, VDRL Slide Qualitative Test with Cerebrospinal Fluid, VDRL Slide Quantitative Test with Cerebrospinal Fluid. The VDRL antigen has been standardized by comparison with an antigen of known reactivity to produce standard reactivity in the tests listed above. The VDRL Buffered Saline is for the preparation of antigen emulsion for the VDRL and the tube flocculation tests. VDRL Test Control Serum Set Venereal Disease Research Laboratory. Rehydrate to 3 ml per vial; Contains: Nontreponemal Antigen Reactive Serum - 1 vial VDRL Weakly Reactive Serum - 1 vial Nontreponemal Antigen Nonreactive Serum - 1vial Aliquant Vials - 3 vials 5-12 BD Diagnostics Diagnostic Systems Catalog 2008/2009

125 Direct Testing Systems & Serology BD Serology Diagnostics BD VZVscan Viral Diagnostic Testing Systems determ. BD VZVscan - Card Test Kit This is a qualitative and quantitative latex agglutination assay which utilizes polystyrene particles sensitized with partially purified, attenuated varicella-zoster virus (VZV) antigens for the detection of total VZV antibodies in human serum. (30 determinations) containing: one 0.5 ml bottle Latex Antigen, Reagent A one 5.0 ml bottle Card Dilution Buffer, Reagent B one 0.5 ml bottle High Reactive Control one 0.5 ml bottle Low Reactive Control one 0.5 ml bottle Nonreactive Control 5 test cards, 12 spot plastic stirrers determ. BD VZVscan - Card Test Kit Varicella-Zoster Virus Antibody Card Test. (100 determinations) containing: one 1.6 ml bottle Latex Antigen, Reagent A one 20 ml bottle Card Dilution Buffer, Reagent B one 0.5 ml bottle High Reactive Control one 0.5 ml bottle Low Reactive Control one 0.5 ml bottle Nonreactive Control 8 test cards, 35 spot plastic stirrers BD Diagnostics Diagnostic Systems Catalog 2008/

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127 Stains & Reagents BD offers a broad line of reagent droppers, diagnostic kits, antigens and antisera, and strep grouping products are conveniently available. These items aid in the identification of Streptococci, Staphylococci, Neisseria gonorrhoeae, Campylobacter, Salmonella, Shigella and a variety of other organisms. Reagents are available for the detection of febrile diseases, phenylketonuria (PKU) and syphilis. The visualization of bacteria by means of staining procedures is an important tool for the microbiologist as an aid in the identification and classification of isolates. BD Diagnostic Systems provides the microbiologist with carefully standardized and reproducible stain reagents in order to ensure accurate and interpretable results. Stains are also available to provide rapid visualization of mycobacteria and provide early detection of positive blood cultures by fluorescence procedures. BD Difco TM & BD BBL TM Antigens, Antisera & Adjuvants 6-2 BD Diagnostic Reagents 6-14 BD Difco TM & BD BBL TM Stains, Droppers & Indicators 6-19 BD Enzymes 6-21 BD TB Stain Kits & Reagents 6-22

128 Stains & Reagents & Antigens, Antisera & Adjuvants Antigens - Harmonized / ml set ml ml ml ml ml ml ml ml ml ml ml Brucella Abortus - Antigen Glycerinated and ready to use. Part of the Febrile Antigen Kit. Febrile Antigen Kit Glycerinated and ready to use. No more mercury based preservatives; eliminates environmental and handling hazards. No dilution or re-hydration required. The kit contains: Brucella abortus Antigen, Brucella Positive Control AMS, Febrile Antigen Negative Control, Proteus OX19 Antigen, Proteus Polyvalent Antiserum, Salmonella Flagellar Poly Antiserum, Salmonella H Antigen a, Salmonella H Antigen b, Salmonella H Antigen d, Salmonella O Antigen Group D, Salmonella Somatic Poly Antiserum. Set of 5 ml vials. Febrile Antigen Negative Control (optional) Febrile Control Serum, Glycerinated, Ready to Use (no dilution or re-hydration required). Francisella Tularensis - Antigen Glycerinated and ready to use. Part of the Febrile Antigen Kit. Proteus OX19 - Antigen Glycerinated and ready to use. Part of the Febrile Antigen Kit. Proteus OX2 - Antigen Glycerinated and ready to use. Part of the Febrile Antigen Kit. Proteus OXK - Antigen Glycerinated and ready to use. Part of the Febrile Antigen Kit. Salmonella Flagellar - Antigen a Part of the Febrile Antigen Kit. Salmonella Flagellar - Antigen b Part of the Febrile Antigen Kit. Salmonella Flagellar - Antigen d Typhoid H. Part of the Febrile Antigen Kit. Salmonella O - Antigen Group A Somatic Part of the Febrile Antigen Kit. Salmonella O - Antigen Group B Somatic Part of the Febrile Antigen Kit. Salmonella O - Antigen Group D Somatic 9-12, Typhoid O. Part of the Febrile Antigen Kit. BBL BBL BBL BBL BBL BBL BBL BBL BBL BBL BBL BBL BBL 6-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

129 Stains & Reagents & Antigens, Antisera & Adjuvants Antigens ml ml ml ml Bordetella Pertussis Antigen Used to demonstrate a positive quality control test in the slide agglutination test. This Antigen is a suspension of a selected strain of B. pertussis containing approximately 0.04 % thimerosal as a preservative. Listeria O Antigen Type 1 (Slide) Quality control of rapid slide test using Listeria O - Antiserum, Type 1, Listeria O Antigen Type 4 (Slide) Quality control of rapid slide test using Listeria O - Antiserum, Type 4, QC Antigen Salmonella O - Group A QC Antigens Salmonella O are used in the quality control testing of Salmonella O Antisera by slide agglutination tests. The use of Salmonella O Antisera in the serologic identification of Salmonella requires the use of quality control test suspensions to verify that the antisera are performing as expected. Most laboratories are required to test antisera with positive and negative controls prior to use. BD Difco QC Antigens Salmonella O are chemically stabilized and inactivated suspensions of known strains of the genus Salmonella designed for use as homologous controls for testing the efficacy of the Salmonella typing antisera employed in routine laboratory procedures. The QC Antigens Salmonella O may also be used to demonstrate a heterologous (negative) control reaction. For use as a negative control, use a QC Antigen Salmonella that contains antigens unrelated to those in the homologous control. Used in the Quality Control of Salmonella O Antisera Poly A (BD / formerly ), Factor 2 (BD / formerly ), Group A Factors 1, 2, 12 (BD / formerly ) & Poly A-I & Vi (BD / formerly ) ml ml ml QC Antigen Salmonella O - Group B Used in the quality control of Salmonella O Antisera Poly A (BD / formerly ), Factor 4 (BD / formerly ), Factors 4, 5 (BD / formerly ), Factor 5 (BD / formerly ), Group B Factors 1, 4,12,27 (BD / formerly ) & Poly A-I & Vi ( / formerly ). QC Antigen Salmonella O - Group C1 Used in the quality control of Salmonella O Antisera Poly B (BD / formerly ), Group C1 Factors 6, 7 (BD / formerly ), Factor 7 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group C2 Used in the quality control of Salmonella O Antisera Poly B (BD / formerly ), Group C2 Factor 6, 8 (BD / formerly ), Factor 8 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). BD Diagnostics Diagnostic Systems Catalog 2008/

130 Stains & Reagents & Antigens, Antisera & Adjuvants Antigens ml ml ml ml ml ml ml ml QC Antigen Salmonella O - Group D Used in the quality control of Salmonella O Antisera Poly A (BD / formerly ), Factor 9 (BD Cat No / formerly ), Group D1 Factors 1, 9, 12 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group E1 Used in the quality control of Salmonella O Antisera Poly A (BD / formerly ), Factor 10 (BD / formerly ), Group E Factors 1, 3, 10, 15, 19, 34 (BD / formerly ), Group E1 Factors 3, 10 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group E2 Used in the quality control of Salmonella O Antisera Poly A (BD / formerly ), Factor 15 (BD / formerly ), Group E Factors 1, 3, 10, 15, 19, 34 (BD / formerly ), Group E2 Factor 3, 15 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group E4 Used in the quality control of Salmonella O Antisera Poly A (BD / formerly ), Factor 19 (BD / formerly ), Group E Factors 1, 3, 10, 15, 19, 34 (BD / formerly ), Group E4 Factor 1, 3, 19 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group F Used in the quality control of Salmonella O Antisera Poly B (BD / formerly ), Group F Factor 11 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group G1 Used in the quality control of Salmonella O Antisera Poly B (BD / formerly ), Factor 22 (BD / formerly ), Group G Factors 13, 22, 23 (36), (37) (BD / formerly ), Group G1 Factors 13, 22, (36) (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group H Used in the quality control of Salmonella O Antisera Poly B (BD / formerly ), Group H Factors 1, 6, 14, 24, 25 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). QC Antigen Salmonella O - Group I Used in the quality control of Salmonella O Antisera Poly C (BD / formerly ), Group I Factor 16 (BD / formerly ) & Poly A-I & Vi (BD / formerly ). 6-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

131 Stains & Reagents & Antigens, Antisera & Adjuvants Antigens ml ml QC Antigen Salmonella O - Group Vi Used in the quality control of Salmonella O Antisera Poly A-I & Vi (BD / formerly ) & Vi (BD / formerly ). QC Antigen Shigella - Group A QC Antigens Shigella are used in the quality control testing of Shigella Antisera Poly by slide agglutination tests. The use of Shigella Antisera Poly in the serologic identification of Shigella requires the use of quality control test suspensions to verify that the antisera are performing as expected. Most laboratories are required to test antisera with positive and negative controls prior to use. BD Difco QC Antigens Shigella are chemically stabilized and inactivated suspensions of known strains of the genus Shigella designed for use as positive controls for testing the efficacy of the Shigella grouping antisera employed in routine laboratory procedures. QC Antigens Shigella may also be used as negative controls by using a heterologous antigen (possessing no common antigen) with a given test antiserum. However, cross-reactivity may occur. Consult appropriate references for further details on cross-reactivity. Used in the quality control of Shigella Antiserum Poly Group A (BD / formerly ) ml ml ml ml ml ml QC Antigen Shigella - Group A1 Used in the quality control of Shigella Antiserum Poly Group A1 (BD / formerly ). QC Antigen Shigella - Group B Used in the quality control of Shigella Antiserum Poly Group B (BD / formerly ). QC Antigen Shigella - Group C Used in the quality control of Shigella Antiserum Poly Group C (BD / formerly ). QC Antigen Shigella - Group C1 Used in the quality control of Shigella Antiserum Poly Group C1 (BD / formerly ). QC Antigen Shigella - Group C2 Used in the quality control of Shigella Antiserum Poly Group C2 (BD / formerly ). QC Antigen Shigella - Group D Used in the quality control of Shigella Antiserum Poly Group D (BD / formerly ). BD Diagnostics Diagnostic Systems Catalog 2008/

132 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera - Harmonized / ml ml ml ml ml ml ml ml ml Brucella Positive Control - Antiserum (AMS) Febrile Control Serum, Glycerinated, Ready to Use (No dilution or re-hydration required). Francisella Tularensis - Antiserum Febrile Control Serum, Glycerinated, Ready to Use (no dilution or re-hydration required). Proteus Polyvalent - Antiserum (OXK, OX2, OX19) Febrile Control Serum, Glycerinated, Ready to Use (no dilution or re-hydration required). Salmonella Flagellar Poly - Antiserum (a, b, d) Febrile Control Serum, Glycerinated, Ready to Use (no dilution or re-hydration required). Salmonella O - Antiserum, Group A Glycerinated, Ready to Use. Salmonella O - Antiserum, Group D Glycerinated and ready to use. Part of the Salmonella O Grouping Kit. Salmonella O - Antiserum, Group H Glycerinated and ready to use. Part of the Salmonella O Grouping Kit. Salmonella Somatic Poly - Antiserum (A, B, D) Febrile Control Serum, Glycerinated, Ready to Use (no dilution or re-hydration required). Shigella Antiserum Poly Group A Glycerinated and ready to use. Part of the Shigella Grouping Kit. BBL BBL BBL BBL BBL BBL BBL BBL BBL 6-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

133 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml Alkalescens-Dispar Antiserum Poly Used for the identification of Alkalescens-Dispar Group of microorganisms by the slide agglutination test. A lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. Bordetella Parapertussis Antiserum Recommended for use in slide agglutination tests for the identification of Bordetella parapertussis. These Antisera are lyophilized, polyclonal rabbit antisera containing approx % thimerosal as a preservative. Bordetella Pertussis Antiserum Recommended for use in slide agglutination tests for the identification of Bordetella pertussis. These Antisera are lyophilized, polyclonal rabbit antisera containing approx % thimerosal as a preservative. E. Coli H Antiserum H7 Tube test for serological confirmation of E. coli O157:H7. A lyophilized, polyclonal rabbit antisera containing approximately 0.04 % thimerosal as a preservative. E. Coli O Antiserum O157 Tube test for serological confirmation of E. coli O157:H7. A lyophilized, polyclonal rabbit antisera containing approximately 0.04 % thimerosal as a preservative. Haemophilus Influenzae Antiserum Poly Recommended for use in slide agglutination tests for serotyping Haemophilus influenzae. These Antisera are stable, lyophilized, polyclonal rabbit antisera containing approximately 0.02 % thimerosal as a preservative. Haemophilus Influenzae Antiserum Type a Haemophilus Influenzae Antiserum Type b Haemophilus Influenzae Antiserum Type c Haemophilus Influenzae Antiserum Type d Haemophilus Influenzae Antiserum Type e Haemophilus Influenzae Antiserum Type f Listeria O Antiserum Type 1 Listeria O Antisera Types 1, 4 and Poly are used for identifying Listeria monocytogenes in the slide and tube agglutination tests. Listeria O Antigens Types 1 and 4 (Slide and Tube) are used as positive controls in the slide and tube agglutination tests, respectively. These are lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. Listeria O Antisera Types 1 and 4 are specific for the respective serotypes of L. monocytogenes while Listeria O Antiserum Poly contains agglutinins for L. monocytogenes serotypes 1 and ml Listeria O Antiserum Type 4 BD Diagnostics Diagnostic Systems Catalog 2008/

134 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml Listeria O Antiserum Poly Neisseria Meningitidis Antiserum Poly Neisseria Meningitidis Antisera are recommended for use in slide agglutination tests for serotyping Neisseria meningitidis. These Antisera are stable, lyophilized, polyclonal rabbit antisera containing approximately 0.02 % thimerosal as a preservative. Neisseria Meningitidis Antiserum Poly 2 Neisseria Meningitidis Antiserum Group A Neisseria Meningitidis Antiserum Group B Neisseria Meningitidis Antiserum Group C Neisseria Meningitidis Antiserum Group D Neisseria Meningitidis Antiserum Group X Neisseria Meningitidis Antiserum Group Y Neisseria Meningitidis Antiserum Group Z Neisseria Meningitidis Antiserum Group Z' Neisseria Meningitidis Antiserum Group W135 Salmonella H - Antiserum a Used in tube agglutination tests for the identification of Salmonella by flagellar (H) antigens. Salmonella H Antisera are lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. They are polyvalent antisera specific for certain flagellar antigens. Each vial of Salmonella H Antisera contains sufficient reagent to perform between tests, depending on the antiserum used. Salmonella H Antisera are either absorbed or unabsorbed specifically for either phase 1 or phase 2 antigens ml ml ml ml ml ml ml Salmonella H - Antiserum b Salmonella H - Antiserum c Salmonella H - Antiserum d Salmonella H - Antiserum eh Salmonella H - Antiserum f Salmonella H - Antiserum h Salmonella H - Antiserum i 6-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

135 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml Salmonella H - Antiserum k Salmonella H - Antiserum m Salmonella H - Antiserum p Salmonella H - Antiserum r Salmonella H - Antiserum s Salmonella H - Antiserum t Salmonella H - Antiserum w Salmonella H - Antiserum x Salmonella H - Antiserum y Salmonella H - Antiserum z Salmonella H - Antiserum z6 Salmonella H - Antiserum z10 Salmonella H - Antiserum z13 Salmonella H - Antiserum z15 Salmonella H - Antiserum z23 Salmonella H - Antiserum z28 Salmonella H - Antiserum z29 Salmonella H - Antiserum z32 Salmonella H - Antiserum EN Complex Salmonella H - Antiserum G Complex Salmonella H - Antiserum L Complex Salmonella H - Antiserum Z4 Complex Salmonella H - Antiserum Poly a-z Salmonella H - Antiserum Poly A (a,b,c,d,i,z10,z29) BD Diagnostics Diagnostic Systems Catalog 2008/

136 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml ml Salmonella H - Antiserum Poly B (eh,en,enx,enz15, and G Complex) Salmonella H - Antiserum Poly C (k,l,r,y,z,z4) Salmonella H - Antiserum Poly D (z35,z36,z37,z38,z39,z41,z42) Salmonella H - Antiserum Poly E (1 Complex, z6) Salmonella H - Antiserum Single Factor 2 Salmonella H - Antiserum Single Factor 5 Salmonella H - Antiserum Single Factor 6 Salmonella H - Antiserum Single Factor 7 Salmonella H - Antiserum Spicer-Edwards 1 Salmonella H Antisera Spicer-Edwards are pooled, polyvalent antisera and additional adjunctive antisera to identify the more commonly occurring H antigens. Salmonella H - Antiserum Spicer-Edwards 2 Salmonella H - Antiserum Spicer-Edwards 3 Salmonella H - Antiserum Spicer-Edwards 4 Salmonella H - Antiserum 1 Complex Salmonella O - Antiserum Factor 2 Used in slide agglutination tests for the identification of Salmonella by somatic (O) antigens. Salmonella O Antisera are lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. They are polyvalent antisera. Each antiserum is specific for certain serogroup antigens. When properly rehydrated and used as recommended, each vial of Salmonella O Antisera contains sufficient reagent for 60 tests. They are prepared with representative strains of these serogroups and are not absorbed. They may crossreact because of shared common O antigens ml ml ml ml ml ml Salmonella O - Antiserum Factor 4 Salmonella O - Antiserum, Factors 4,5 Salmonella O - Antiserum Factor 5 Salmonella O - Antiserum Factor 7 Salmonella O - Antiserum Factor 8 Salmonella O - Antiserum Factor BD Diagnostics Diagnostic Systems Catalog 2008/2009

137 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml Salmonella O - Antiserum Factor 10 Salmonella O - Antiserum Factor 12 Salmonella O - Antiserum Factor 14 Salmonella O - Antiserum Factor 15 Salmonella O - Antiserum Factor 19 Salmonella O - Antiserum Factor 20 Salmonella O - Antiserum Factor 22 Salmonella O - Antiserum Factor 23 Salmonella O - Antiserum Factor 25 Salmonella O - Antiserum Factor 27 Salmonella O - Antiserum Factor 34 Salmonella O - Antiserum Group A Factors 1, 2, 12 Salmonella O - Antiserum Group B Factors 1, 4, 5, 12 Salmonella O - Antiserum Group B Factors 1, 4, 12, 27 Salmonella O - Antiserum Group C1 Factors 6, 7 Salmonella O - Antiserum Group C2 Factors 6, 8 Salmonella O - Antiserum Group C3 Factors (8), 20 Salmonella O - Antiserum Group D1 Factors 1, 9, 12 Salmonella O - Antiserum Group D2 Factors (9), 46 Salmonella O - Antiserum Group E Factors 1, 3, 10, 15, 19, 34 Salmonella O - Antiserum Group E1 Factors 3, 10 Salmonella O - Antiserum Group E2 Factors 3, 15 Salmonella O - Antiserum Group E3 Factors (3), (15), 34 Salmonella O - Antiserum Group E4 Factors 1, 3, 19 BD Diagnostics Diagnostic Systems Catalog 2008/

138 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml ml Salmonella O - Antiserum Group F Factor 11 Salmonella O - Antiserum Group G Factors 13, 22, 23, (36), (37) Salmonella O - Antiserum Group G1 Factors 13, 22, (36) Salmonella O - Antiserum Group G2 Factors 1, 13, 23, (37) Salmonella O - Antiserum Group H Factors 1, 6, 14, 24, 25 Salmonella O - Antiserum Group I Factor 16 Salmonella O - Antiserum Group J Factor 17 Salmonella O - Antiserum Group K Factor 18 Salmonella O - Antiserum Group L Factor 21 Salmonella O - Antiserum Group M Factor 28 Salmonella O - Antiserum Group N Factor 30 Salmonella O - Antiserum Group O Factor 35 Salmonella O - Antiserum Poly A-I & Vi Salmonella O - Antiserum Poly A (A, B, D, E1, E2, E3, E4, & L) Salmonella O - Antiserum Poly B (C1, C2, F, G, & H) Salmonella O - Antiserum Poly C (I, J, K, M, N, & O) Salmonella O - Antiserum Poly D (P, Q, R, S, T, & U) Salmonella O - Antiserum Poly E (V, W, X, Y, & Z) Salmonella O - Antiserum Poly F (Groups 51-55) Salmonella O - Antiserum Poly G (Groups 56-61) Salmonella Antiserum Vi Used in slide agglutination tests for the identification of Salmonella Vi. Salmonella Vi Antisera are lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. When properly rehydrated and used as recommended, each vial of Salmonella Vi Antisera contains sufficient reagent for 60 tests. Salmonella Vi Antisera are prepared with representative strains of these serogroups and are not absorbed BD Diagnostics Diagnostic Systems Catalog 2008/2009

139 Stains & Reagents & Antigens, Antisera & Adjuvants Antisera ml ml ml ml ml ml ml ml ml ml Shigella Antiserum Poly Group A Used for the identification of Shigella species by the slide agglutination test. A lyophilized, polyclonal rabbit antisera containing approximately 0.04 % Thimerosal as a preservative. Shigella Antiserum Poly Group A1 Shigella Antiserum Poly Group B Shigella Antiserum Poly Group C Shigella Antiserum Poly Group C1 Shigella Antiserum Poly Group C2 Shigella Antiserum Poly Group D Vibrio Cholerae Antiserum Inaba Vibrio Cholerae Antiserum Inaba is a monospecific absorbed antiserum. Vibrio Cholerae Antiserum Ogawa Vibrio Cholerae Antiserum Ogawa is a monospecific absorbed antiserum. Vibrio Cholerae Antiserum Poly Recommended for use in slide agglutination tests for the identification and serotyping of Vibrio cholerae. Rabbit serum for slide agglutination test. Contains agglutinins for V. cholerae, inaba and ogawa. Adjuvants x 10 ml x 10 ml x 10 ml x 100 mg x 100 mg g Adjuvant, Complete (Freund) Suspension of 5 mg M. butyricum in a mixture of paraffin oil and an emulsifying agent. Adjuvant, Complete H37 Ra Suspension of 10 mg M. tuberculosis in a mixture of paraffin oil and an emulsifying agent. Adjuvant, Incomplete (Freund) M. butyricum (Desiccated) Killed M. butyricum for use in adjuvants. M. Tuberculosis H37 Ra (Desiccated) Killed M. tuberculosis H37 Ra for use in adjuvants. Mucin Bacteriological Derived from hog gastric mucosa. Used to increase bacterial virulence upon injection. BD Diagnostics Diagnostic Systems Catalog 2008/

140 Stains & Reagents BD Diagnostic Reagents Diagnostic Reagent & Stain Droppers Each ampulle contains 0.5 ml ampules ampules ampules ampules ampules ampules Acridine Orange Acridine Orange Reagent Droppers are used for fluorescent microscopic detection of microorganisms in direct smears. Acridine orange, a fluorochrome stain buffered at a low ph, differentiates between bright orange-stained bacteria and pale green to yellow-stained human cells and tissue. Each dropper contains sufficient reagent to stain one slide. Contains 0.5 ml of a 0.01 % solution of [3,6-bis (dimethylamino) acridine hydrochloride] in a 0.5 M acetate buffer. Calcofluor White Calcofluor White Reagent Droppers are used in the rapid fluorescent microscopic detection of fungi in direct smears. It is a nonspecific fluorochrome stain that binds to fungi and, depending upon the filter system employed, fluoresces either an apple green or blue white color when exposed to light of the appropriate wavelength. It may be used on fresh, frozen, fixed, paraffinimbedded, and clinical specimens. It can be used in the detection of Pneumocystis carinii and other opportunistic fungal parasites in bronchoalveolar lavage (BAL) specimens and aspirates from immunosuppressed individuals such as AIDS patients. Contains 0.5 ml of a 0.05 % solution of calcofluor white in distilled water. Catalase Catalase Reagent Droppers are used in a qualitative procedure for determining catalase activity by bacteria. The enzyme catalase is present in most aerobic and facultatively anaerobic bacteria, with the major exception of streptococci and enterococci. The enzyme catalase decomposes hydrogen peroxide into water and oxygen. Catalase Reagent Droppers contain a hydrogen peroxide solution of approximately 3 % (2.5 % to 3.5 %). This reagent has been frequently cited as a standard method for performing the catalase test. Contains (approximately) 3 % Hydrogen Peroxide and 0.05 % or less of stabilizer. Desoxycholate Desoxycholate Reagent Droppers are used for the presumptive differentiation of pneumococci from other gram-positive cocci by the bile solubility test. Sodium desoxycholate is a salt of purified bile acid and can be used in lower concentrations than other salts of bile acids. It is soluble in water and lyses pneumococcal cells treated with a 10 % solution. The bile solubility test may be performed by adding the sodium desoxycholate solution to a cell suspension or directly to colonies on a blood agar plate. Each dropper is good for one day's use after ampule has been broken. Contains 0.5 ml of 10 % aqueous solution of sodium desoxycholate. DMACA Indole DMACA Indole Reagent Droppers are for the detection of indole production as an aid in the identification of aerobic, anaerobic, or facultatively anaerobic organisms. They are used in a rapid spot test for determining the ability of bacteria to produce indole by the deamination of tryptophan. The production of indole has been widely used as an aid in differentiating certain genera and species within the same genus. p- Dimethylaminocinnamaldehyde (DMACA) has been shown to be sensitive to the production of indole. In addition, the color change with DMACA is both rapid and long lasting, making results easier to interpret than with more traditional reagents. Each dropper is good for one day's use after the ampule has been broken. Contains 0.5 ml of aqueous 1 % p-dimethylaminocinnamaldehyde in 10 % hydrochloric acid. Dobell & O'Connor Iodine Dobell & O'Connor Iodine Stain Droppers have a variety of uses as a microbiological stain. Among them are staining the trophozoite, cyst, and egg stages of intestinal parasites. Use of Dobell & O'Connor Iodine Stain Droppers aid the visualization of the components of specimens observed microscopically. The selection of the appropriate technique depends upon the specimen. Contains 0.5 ml of solution consisting of iodine 1 %, potassium iodide 2 %, and water BD Diagnostics Diagnostic Systems Catalog 2008/2009

141 Stains & Reagents BD Diagnostic Reagents Diagnostic Reagent & Stain Droppers Each ampulle contains 0.5 ml ampules ampules ampules ampules ampules Ferric Chloride Ferric Chloride Reagent Droppers are intended for use in the differentiation of microorganisms capable of phenylalanine deamination. They are used to determine if a specific microorganism is capable of producing phenylpyruvic acid from phenylalanine deamination. Most members of the genera Proteus and Providencia are positive whereas most Escherichia and Citrobacter species are negative. Ferric Chloride Reagent Droppers contain 0.5 ml of 10 % ferric chloride in aqueous solution. Flagella Flagella Stain Droppers are used for demonstrating bacterial flagella and their rrangement on the cell. Due to their narrow diameter, bacterial flagella cannot be seen with the light microscope without the use of special stains. The presence of bacterial flagella, flagellum length, shape, curvature, number per cell and arrangement on the cell are useful in the identification of motile bacteria. The Flagella Stain is based on the tannic acid-crystal violet formula first described by Ryu and later by Kodaka, Armfield, Lombard and Dowell. The presence of flagella is especially useful in the identification of nonfermentative, gram-negative bacilli and anaerobic bacteria. Contains 0.6 % Crystal Violet in Ethanol, 2.0 % Tannic Acid, 2.5 % Phenol and 5.7 % Aluminum Potassium Sulfate. India Ink India Ink Reagent Droppers are used to enhance the microscopic detection of Cryptococcus spp. in wet preparations. India ink facilitates the visualization of cryptococcal polysaccharide capsules. The capsular material of cryptococci displaces the colloidal carbon particles of the ink so that the capsule appears as a clear halo around the microorganisms against a black background. India Ink Reagent Droppers may be used to stain pus, exudate, tissue, sputum and sediment of centrifuged urine and cerebral spinal fluid (CSF) specimens. The India ink smear is particularly useful for CSF specimens because Cryptococcus neoformans is the most common cause of fungal central nervous system (CNS) infections throughout the world. The appearance of encapsulated yeast forms in an India ink smear from CSF is diagnostic for cryptococcal meningitis. Contains 0.5 ml of an aqueous suspension of India ink with suspending agent and preservative. Indole Indole Reagent Droppers (modified Kovacs' reagent) are used in determining the ability of bacteria to produce indole by the deamination of tryptophan. The production of indole has been widely used as an aid in differentiating certain genera and species within the same genus. Indole Reagent may be used for tube or extraction procedures or with a variety of biochemical kits requiring Kovacs' or Ehrlich's reagents. Each dropper is good for one day's use after the ampule has been broken. Contains 0.5 ml of 5 % p-dimethylaminobenzaldehyde dissolved in a solution of 25 % hydrochloric acid and 75 % isobutyl alcohol. Lactophenol Cotton Blue Lactophenol Cotton Blue Stain Droppers are intended for use as a stain in the examination of fungi. The solution can be used in wet mounts in the examination of yeasts and molds and serves as both a mounting fluid and a stain. Contains 0.5 ml of lactophenol cotton blue solution consisting of glycerol 40 %, phenol 20 %, lactic acid 20 %, cotton blue dye (Poirrier's blue) 0.05 %, and water. BD Diagnostics Diagnostic Systems Catalog 2008/

142 Stains & Reagents BD Diagnostic Reagents Diagnostic Reagent & Stain Droppers Each ampulle contains 0.5 ml ampules Methylene Blue Loeffler Methylene Blue Loeffler Stain Droppers are used for presumptive identification of Corynebacterium diphtheriae and as an adjunct to the Gram stain. It is used as an adjunct to the Gram stain, especially in the staining of gram-negative bacteria such as Haemophilus influenzae and Neisseria species. The stain can reveal the morphology of fusiform bacteria and spirochetes (from oral infections such as Vincent's angina) that may be difficult to see with the Gram stain. The stain is also used to detect the presence of fecal white blood cells that may show the presence of invasive bowel disease. Methylene Blue Loeffler Stain Droppers can be used as a counterstain with Flagella Stain Droppers, which are recommended for staining the flagella of bacteria. Methylene Blue Loeffler Stain Droppers are supplied in a hermetically sealed ampule for improved stability of the reagent prior to use. Contains 0.3 g Methylene Blue, 30 ml Alcohol, 95 %, 0.1 ml Potassium Hydroxide, 10 %, and 100 ml Distilled Water ampules ampules ampules ampules ampules Ninhydrin Ninhydrin Reagent Droppers are used in the determination of the hippurate reaction to aid in the identification of certain bacteria. The hydrolysis of sodium hippurate is a qualitative test useful in the identification of Streptococcus agalactiae (group B ß-hemolytic streptococci), Campylobacter jejuni, Gardnerella vaginalis, Listeria sp. and other aerobic bacteria. The presence of the enzyme hippurate hydrolase is visualized by the purple color formed when ninhydrin reagent oxidizes the amino acid produced during hippurate hydrolysis. Contains 0.5 ml of 3.5 % ninhydrin in 50 % aqueous ethanol. Nitrate Reagent A For use in determining the ability of bacteria to reduce nitrate to nitrite or free nitrogen gas. The Enterobacteriaceae, many other gram-negative bacteria, mycobacteria and fungi reduce nitrate to nitrite. The nitrate-reducing characteristic of a species is constant for certain genera and species. The Nitrate A and B Reagents, when added in equal parts, indicate the presence of a catabolic end product or the absence of nitrate in the medium. Nitrate A Reagent Droppers contain 0.5 ml of 0.8 % Sulfanilic Acid in 5N Acetic Acid. Nitrate Reagent B For use in determining the ability of bacteria to reduce nitrate to nitrite or free nitrogen gas. The Enterobacteriaceae, many other gram-negative bacteria, mycobacteria and fungi reduce nitrate to nitrite. The nitrate-reducing characteristic of a species is constant for certain genera and species. The Nitrate A and B Reagents, when added in equal parts, indicate the presence of a catabolic end product or the absence of nitrate in the medium. Nitrate B Reagent Droppers contain 0.5 ml of 0.6 % N,N-Dimethyl-alpha-naphthylamine in 5N Acetic Acid. Oxidase Oxidase Reagent Droppers are used in the Kovacs oxidase test as a qualitative reaction in the identification of nonfermenters and miscellaneous gram-negative bacteria. The oxidase test is based on the production of an enzyme called indophenol oxidase. This enzyme oxidizes a redox dye (present in the reagent) which results in a color change of yellow to dark purple. Contains 0.5 ml of a 1 % aqueous solution of N,N,N',N'-tetramethylp-phenylenediamine dihydrochloride which has been formulated with agents to ensure maximum stability. 10 % Potassium Hydroxide 10 % Potassium Hydroxide Reagent Droppers are intended for use in the examination of direct smears for fungal elements. 10 % potassium hydroxide (KOH) facilitates the clearing of specimens for enhanced microscopic observation of fungi without altering the fungal elements present. It is most useful with skin, hair, nail and sputum specimens. Each dropper is good for one day s use after the ampule has been broken. Contains 0.5 ml of 10 % KOH with 1 % dimethyl sulfoxide BD Diagnostics Diagnostic Systems Catalog 2008/2009

143 Stains & Reagents BD Diagnostic Reagents Diagnostic Reagent & Stain Droppers Each ampulle contains 0.5 ml ampules ampules ampules PYR PYR Reagent Droppers are used in the rapid presumptive identification of group A ß-hemolytic streptococci and group D enterococci. They are used in a rapid test for determining the ability of bacteria to produce pyrrolidonyl peptidase. The production of pyrrolidonyl peptidase (PYRase) has been widely used as an aid in differentiating certain streptococci species. Following incubation of organisms with a PYR substrate, the reagent (cinnamaldehyde) is added. Color development indicates a positive reaction. The color change is both rapid and long lasting, making results easy to interpret. Contains 0.5 ml of % p-dimethylaminocinnamaldehyde in acetic acid. Voges-Proskauer A Voges-Proskauer Reagent Droppers are intended for use in the Voges-Proskauer test, one of several qualitative tests used to distinguish between members of the Enterobacteriaceae. The Voges-Proskauer test is one of the series known as the IMViC tests. The letters stand for Indole, Methyl Red, Voges-Proskauer, and Citrate. (The "i" is inserted for euphony.) They are used primarily to distinguish between the coliforms, but may be used for other organisms in the Enterobacteriaceae family. Specifically, the Voges-Proskauer test detects the presence of acetylmethylcarbinol. This compound is produced by certain microorganisms during growth in a buffered peptone-glucose broth, specifically MR-VP broth. Contains 0.5 ml of 5 % wt/vol alphanaphthol in absolute alcohol. Voges-Proskauer B Voges-Proskauer Reagent B Droppers contain 0.5 ml of 40 % wt/vol potassium hydroxide in distilled water. BD Coagulase Plasma Coagulase Plasmas are used in qualitative procedures to determine the pathogenicity of staphylococci in various tests x 3 ml x 15 ml x 25 ml Coagulase Plasma Used to qualitatively determine the pathogenicity of staphylococci using the direct tube method. Coagulase Plasma, Rabbit is lyophilized rabbit plasma with 0.85 % sodium citrate and 0.85 % sodium chloride, approximately. Reconstitutes to 3 ml / 15 ml / 25 ml for Direct Tube Method x 3 ml x 15 ml x 25 ml Coagulase Plasma with EDTA Used to qualitatively determine the pathogenicity of staphylococci using the direct tube method. Coagulase Plasma, Rabbit with EDTA is lyophilized rabbit plasma with 0.15 % EDTA (ethylenediaminetetraacetic acid) and 0.85 % sodium chloride, approximately. Reconstitutes to 3 ml / 15 ml / 25 ml for Direct Tube Method. BD Diagnostics Diagnostic Systems Catalog 2008/

144 Stains & Reagents BD Diagnostic Reagents & Fluorescent Antibodies & Reagents ml ml x 10 g g FA Bordetella Parapertussis For use in the direct fluorescene identification of B. parapertussis. FA Bordetella Pertussis For use in the direct fluorescence identification of B. pertussis. FA Buffer (Dried) A phosphate-buffered saline (PBS) which, upon rehydration, yields a 0.85 % NaCI solution buffered to ph 7.2. FA Buffer, Dried is used in preparing: Reactive Control Serum (4+) - Unabsorbed Minimally Reactive Control Serum (1+) Nonreactive Control Serum (N) Nonspecific Staining Control - Unabsorbed FTA Hemagglutination Buffer FTA Hemagglutination Buffer (Phosphate Buffered Saline, ph 7.2) is used in the FTA-ABS test and other serological procedures as a diluent and for washing slide preparations BD Diagnostics Diagnostic Systems Catalog 2008/2009

145 Stains & Reagents - - Stains, Droppers & Indicators BD Gram Stain Kits & Reagents Gram Stain Kits and Reagents are used to stain microorganisms from cultures or specimens by the differential Gram method kit Gram Stain Kit (Stabilized) Used to differentiate intact, morphologically similar bacteria into two groups based on cell color after staining. In addition, cell form, size and structural details are evident. Such preliminary information provides important clues to the type of organism(s) present and the further techniques required to characterize them. Because inorganic iodine is rapidly oxidized and loses its effectiveness as a mordant, the Gram Stain Kit ( ) differs from Gram's original formulation by offering a more stable organic iodine complex, L-polyvinylpyrrolidone-iodine. Each kit contains 1 bottle (250 ml) each of: Gram Crystal Violet, Gram Iodine (Stabilized), Gram Decolorizer & Gram Safranin kit Gram Stain Kit (Unstabilized) For the differential staining of bacteria. Each kit contains 1 bottle (250 ml) each of: Gram Crystal Violet, Gram Iodine (Unstabilized), Gram Decolorizer & Gram Safranin x 250 ml l x 250 ml l x 250 ml l x 250 ml l x 250 ml l x 50 tests x 250 ml l Gram Basic Fuchsin (COUNTERSTAIN). For staining microorganisms by the differential Gram method. Gram Crystal Violet (PRIMARY STAIN). For staining microorganisms by the differential Gram method. Gram Decolorizer For staining microorganisms by the differential Gram method. Gram Iodine (Stabilized) (MORDANT). For staining microorganisms by the differential Gram method. Gram Iodine (Unstabilized) (MORDANT) Working solution prepared from Gram Diluent and Gram Iodine 100X. For staining microorganisms by the differential Gram method. Gram Quality Control Slide Gram Stain QC Slides. Conventional 1 x 3 microscope slides imprinted with 10 squares. One square contains control organisms Staphylococcus aureus and Escherichia coli. Nine squares are available for staining test isolates. Gram Slides, individually wrapped, sufficient for 50 tests. Gram Safranin (COUNTERSTAIN) For staining microorganisms by the differential Gram method. BD Diagnostics Diagnostic Systems Catalog 2008/

146 Stains & Reagents - - Stains, Droppers & Indicators Indicators & Liquids for Various Stains g g g g g ml Bromcresol Purple Brom Thymol Blue Crystal Violet - Gentian Violet Phenol Red For use in preparing microbiological culture media. TTC Solution (1 %, Sterile) TTC Solution 1% (Triphenyltetrazolium Chloride) is ready for use in the preparation of culture media. For use with KF Streptococcus Agar ( ) : Bottle 25 g : prepared Tube 30 ml BD Diagnostics Diagnostic Systems Catalog 2008/2009

147 Stains & Reagents BD Enzymes & Enzymes g x 20 ml x 20 ml ml x 100 ml x 20 ml ml x 20 ml Chicken Pancrease Chicken Pancrease - Desiccated, used in enzymatic liberation of folic acid from ist conjugated state. Bottle 10 g. Penase Penase is a beta-lactamase which hydrolyze the beta-lactam ring in penicillins, thereby, inactivating the antimicrobial properties of penicillin. Used in media for sterility testing of penicillins and for determining microbial counts of materials containing penicillin. Penase Concentrate Penase Concentrate is a beta-lactamase which hydrolyze the beta-lactam ring in penicillins, thereby, inactivating the antimicrobial properties of penicillin. Used in media for sterility testing of penicillins and for determining microbial counts of materials containing penicillin. Penicillinase Penicillinase: units/ml. Enzyme preparation used to neutralize penicillin and to permit growth of organisms ordinarily inhibited by the antibiotic. 20 ml per Tube. Penicillinase Concentrate Penicillinase Concentrate: units/ml. Enzyme preparation used to neutralize penicillin and to permit growth of organisms ordinarily inhibited by the antibiotic. BD Diagnostics Diagnostic Systems Catalog 2008/

148 Stains & Reagents BD TB Stain Kits & Reagents BD Rapid & Cold Acid-Fast Procedure (TB Quick Stain) kit TB Quick Stain - Kit For a more rapid version of staining mycobacteria by the cold acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Quick Stain Carbolfuchsin & TB Quick Stain Methylene Blue x 250 ml x 250 ml TB Quick Stain - Carbolfuchsin Individual component for TB Quick-Stain-Kit ( ) for a more rapid version of staining mycobacteria by the cold acid-fast procedure. TB Quick Stain - Methylene Blue Individual component for TB Quick Stain Kit ( ) fro a more rapid version of staining mycobacteria by the cold acid-fast procedure. BD Ziehl-Neelsen (Hot) Acid-Fast Procedure kit TB Stain Kit ZN For staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Carbolfuchsin ZN, TB Decolorizer & TB Methylene Blue x 250 ml x 250 ml x 250 ml TB Carbolfuchsin ZN Individual component of the TB Stain Kit ZN ( ) for staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. TB Decolorizer Individual component of the TB Stain Kit ZN ( ) and TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) and Ziehl-Neelsen (hot) acid-fast procedures. TB Methylene Blue Individual component of the TB Stain Kit ZN ( ) for staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. May also be used for staining mycobacteria by the Kinyoun (cold) acid-fast procedure BD Diagnostics Diagnostic Systems Catalog 2008/2009

149 Stains & Reagents BD TB Stain Kits & Reagents BD Kinyoun (Cold) Acid-Fast Procedure kit TB Stain Kit K For staining mycobacteria by the Kinyoun (cold) acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Carbolfuchsin KF, TB Decolorizer & TB Brilliant Green K x 250 ml x 250 ml x 250 ml TB Brilliant Green K Individual component of the TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) acid-fast procedures. May be used also for staining mycobacterial by the Ziehl- Neelsen (hot) acid-fast procedures. TB Carbolfuchsin KF Individual component of the TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) acid-fast procedure. TB Decolorizer Individual component of the TB Stain Kit ZN ( ) and TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) and Ziehl-Neelsen (hot) acid-fast procedures. BD Diagnostics Diagnostic Systems Catalog 2008/

150 Stains & Reagents BD TB Stain Kits & Reagents BD TB Fluorescent Stain Kits kit TB Fluorescent Stain Kit M For staining mycobacteria by the Morse, Blair, Weiser and Sproat fluorescent procedure. Each kit contains 1 bottle (250 ml) each of TB Auramine M, TB Decolorizer TM & TB Potassium Permanganate kit TB Fluorescent Stain Kit T For staining mycobacteria by the Truant, Brett and Thomas fluorescent procedure. Each kit contains 1 bottle (250 ml) each of TB Auramine-Rhodamine T, TB Decolorizer TM & TB Potassium Permanganate ml x 250 ml x 250 ml x 250 ml x 250 ml x 250 ml Acridine Orange Stain For detecting microorganisms in direct smears by the fluorescent staining technique. Recommended for use in the fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. It is particularly useful in the rapid screening of normally sterile specimens, such as cerebrospinal fluid, where few organisms may be present and in the rapid examination of blood smears or smears containing proteinaceous material where differentiation of organisms from background material may be more difficult. TB Auramine M Individual component of the TB Fluorescent Stain Kit M ( ) for staining mycobacteria by the Morse, Blair, Weiser and Sproat fluorescent procedure. TB Auramine-Rhodamine T Individual component of the TB Fluorescent Stain Kit T ( ) for staining mycobacteria by the Truant, Brett and Thomas fluorescent procedure TB Decolorizer TM Individual component of the TB Fluorescent Stain Kits M and T (Cat. Nos and ) for staining mycobacteria by the Truant, Brett and Thomas and the Morse, Blair, Weiser and Sproat fluorescent procedures. TB Potassium Permanganate Individual component of the TB Fluorescent Stain Kits M and T (Cat. Nos and ) for staining mycobacteria by the Truant, Brett and Thomas and the Morse, Blair, Weiser and Sproat fluorescent procedures. Acid-Fast Procedures Accessories & Supplies pcs. Acid Fast Bacilli (AFB) Quality Control Slide The Acid fast Bacilli (AFB) QC Slides are conventional 1 x 3 microscope slides imprinted with two circles. One circle is an acid-fast positive control with Staphylococcus aureus and Bacillus subtilis plus inactivated Mycobacterium tuberculosis H37Ra. The other circle is a negative control with an unstained mixture of non-acid-fast cocci (Staphylococcus aureus) and bacilli (Bacillus subtilis). AFB Slides, individually wrapped, sufficient for 50 tests BD Diagnostics Diagnostic Systems Catalog 2008/2009

151 Identification & Susceptibility Testing Identification systems are utilized to obtain the identification (genus and species) of an organism. These systems contain fluorogenic and chromogenic substrates. When the organism comes into contact with the substrates, the organism either reacts with the substrate (positive reaction) or there is no reaction (negative reaction). When the positive and negative reactions are combined, the identification of the organism is determined. Susceptibility systems are utilized to determine what antimicrobics will be most effective in treating an organism. The organism is tested against various concentrations of antimicrobics, determining the organism s resistance (ineffective) or susceptibility (effective) to the antimicrobics. The identification and susceptibility of an organism is information that the physician requires from the laboratory, which is used when determining patient treatment. BD TM BBL TM Prompt System BD Manual Identification BD Taxo TM Manual Differentiation 7-7 BD Sensi-Disc TM Manual Susceptibility Testing 7-12 BD Phoenix TM Automated Identification & Susceptibility Testing 7-24 BD Microtrol TM Qualtiy Control Organisms 7-34

152 Identification & Susceptibility Testing Prompt System Prompt System Prompt Inoculation System The BBL Prompt Inoculation System is used to prepare standardized suspensions of bacteria for the Bauer-Kirby disc diffusion antimicrobial susceptibility test procedure. The system may be used for rapidly growing bacteria such as Enterobacteriaceae, Staphylococcus spp., Pseudomonas aeruginosa, Acinetobacter spp., enterococci and some nonenterococcal. It also may be used for Haemophilus influenzae. The BBL Prompt Inoculation System is a device that allows direct standardization of inoculum without adjustment of turbidity or preincubation.9-11 This method is considered acceptable for routine testing purposes.4 It has been shown that the Prompt system is also satisfactory for preparing inoculum for H. influenzae in the Bauer-Kirby procedure.12 The Prompt Inoculation System consists of an inoculation wand and a tube of saline. The wand is a polypropylene rod attached to a stopper. At the tip of the wand are cross-hatched grooves designed to hold a specific number of bacteria. The cell suspending solution, consisting of 1 ml of sterile saline, is provided in a plastic tube with a snap off cap. Contains 60 tubes and 62 inoculations wands. This product is not CE marked. 7-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

153 Identification & Susceptibility Testing BD Manual Identification BD Crystal Test Kits tests/kit BD Crystal - Anaerobe ID Kit Intended for the identification of clinically significant anaerobic bacteria in 4 hours. Separate databases for Schaedler Blood Agar, CDC Anaerobe Blood Agar and Alternate Blood Agars are included for improved specificity. The kit contains: 20 panel lids, 20 panel bases, 20 inoculum tubes, 2 incubation trays as well as 1 result pad tests/kit BD Crystal - Enteric/Nonfermenter ID Kit For the identification of aerobic gramnegative bacteria that belong to the family Enterobacteriaceae as well as some of the more frequently isolated glucose fermenting and nonfermenting gram-negative bacilli. This kit is a miniaturized identification method. Many of the tests used are modifications of classical methods. These include tests for fermentation, oxidation, degradation and hydrolysis of various substrates. In addition, there are chromogen linked substrates to detect enzymes that microbes use to metabolize various substrates. The kit contains: 20 panel lids, 20 panel bases, 20 inoculum tubes, 2 incubation trays as well as 1 result pad tests/kit BD Crystal - Gram-Positive ID Kit This system is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated aerobic gram-positive bacteria. The kit contains: 20 panel lids, 20 panel bases, 20 inoculum tubes, 2 incubation trays as well as 1 result pad tests/kit BD Crystal - Neisseria/Haemophilus ID Kit This Kit is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated Neisseria and Haemophilus as well as several other fastidious bacteria. The kit identifies 35 organisms from multiple media. The kit contains: 20 panel lids, 20 panel bases, 20 inoculum tubes, 2 incubation trays as well as 1 result pad. BD Diagnostics Diagnostic Systems Catalog 2008/

154 Identification & Susceptibility Testing BD Manual Identification BD Crystal Test Kits tests/kit BD Crystal - Rapid Gram-Positive ID Kit This System is a miniaturized identification method employing modified conventional, fluorogenic and chromogenic substrates. It is intended for the identification of frequently isolated aerobic gram-positive bacteria. The kit identifies 88 gram-positive organisms from multiple media. The kit congains: 20 panel lids, 20 panel bases, 20 inoculum tubes, 2 incubation trays as well as 1 result pad tubes tubes BD Crystal - Enteric/Stool ID Inoculum Fluid Additional ID inoculum broth tubes for use with BD Crystal E/NF ( ). BD Crystal - Inoculum Fluid Additional ID inoculum broth tubes for use with: BD Crystal ANR ( ), Gram-Positive ( ), Rapid Gram-Positive ( ) & Neisseria/Haemophilus ID kits ( ). 7-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

155 Identification & Susceptibility Testing BD Manual Identification BD Crystal & BD CrystalSpec AutoReader & Accessories BD Crystal - AutoReader The BD Crystal AutoReader along with the BD Crystal Mind software is used for the automated, consistent, and reproducible reading of all BD Crystal panels without subjective interpretation. One panel reading takes approx. 10 sec. The compact BD Crystal AutoReader plus the reagent-free BD Crystal ID kits provide identification of over 500 bacteria, now with automated interpretation. BD Crystal - MIND Software The BD Crystal MIND (Microbiology Interactive Database) software is designed to identify clinically relevant bacteria. BD Crystal MIND provides identification of the unknown organism run in a panel by mathematically interpreting the BD Crystal profile number and off-line test results and evaluating the results against the organisms contained in the appropriate CrystalMind database. For use with BD Crystal AutoReader or alone for manual reading. BD PhoenixSpec - Nephelometer A battery-powered, portable device designed for measuring inoculum density in McFarland units. The device is accurate from McFarland units in increments of 0.1 units. The instrument can be used for any laboratory procedure that requires inoculum density adjustments within this range. Used with BD PhoenixSpec Calibrator Kit, BD PhoenixSpec - Calibration Standards One set contains a BD Phoenix calibration standard (0.25 / 0.5 / 1.0 / 4.0 McFarland). Used for the proper calibration of a BD Phoenix Nephelometer. BD CrystalSpec - Standards One set contains a BD CrystalSpec calibration blank (0 McFarland) and a BD CrystalSpec calibration standard (2.0 McFarland). Used for the proper calibration of a BD CrystalSpec Nephelometer. BD Crystal - Color Reaction Charts Used for the manual interpretation of the color reactions of the various BD Crystal identification kits. BD Crystal - Panel Viewer The BD Crystal Panel Viewer can be used for the manual interpretation of all BD Crystal kits. With integrated UV lamp for the evaluation of chromogenic and fluorogenic reactions. European Model, 220 V, 50 Hz. BD Crystal - Panel Viewer Longwave UV Tube UV Lamp for BD Crystal Panel Viewer ( ). BD Crystal - Panel Viewer White Light Tube BD Crystal - Reference Panel For quality control of the BD Crystal AutoReader. BD Crystal - Anaerobe Manual Codebook BD Diagnostics Diagnostic Systems Catalog 2008/

156 Identification & Susceptibility Testing BD Manual Identification BD Enterotube Testing Systems tubes tubes BD Enterotube II Prepared multimedia tube for the rapid identification of Enterobacteriaceae. Features a self contained compartmented tube containing 12 different conventional media. Allows identification of Enterobacteriaceae (29 taxa). Incubation time is hrs at C. BD Oxi/Ferm Tube II Prepared multimedia tube for the rapid differential identification of gram-negative oxidativefermentative bacteria. Features a self contained compartmented tube containing 12 different conventional media with only one off-line test (Oxidase). Allows identification of Gram-negative non- fermenting bacteria (22 taxa). Incubation time is 48 hrs at C. Enterotube II - Codebook For analysis either with or without VP (Voges - Proskauer - Reaction). BD DrySlide A dry alternative for rapid testing with microbiological biochemical reagents slides BD DrySlide - Indole BD DrySlide Indole is used for determining the indole reaction of bacteria. The indole test is a qualitative procedure for determining the ability of bacteria to produce indole by reductive deamination of tryptophan. Vracko and Sherris showed that the indole test can be performed using filter paper moistened with p-dimethyl-aminobenzaldehyde (DMABA), an effective indicator of indole production. BD DrySlide Indole is a disposable slide with four filter paper reaction areas containing 5 % p- dimethylaminobenzaldehyde (DMABA); 25 x 3 slides slides slides BD DrySlide - Nitrocefin Used for detecting ß-lactamase production by bacteria. BD DrySlide Nitrocefin employs nitrocefin, a cephalosporin compound first described by Glaxo Research (Middlesex, England), in the chromogenic cephalosporin test methodology. BD DrySlide - Oxidase Used for determining the oxidase reaction of bacteria; 25 x 3 slides. The oxidase test is a qualitative procedure for determining the presence or absence of cytochrome c oxidase activity in bacteria. This activity is dependent on the presence of an intracellular cytochrome oxidase system that catalyzes the oxidation of cytochrome c by molecular oxygen, which then serves as the terminal electron acceptor in the organism's electron transport system. Organisms containing cytochrome c as part of their respiratory chain are oxidase positive and turn the reagent purple; organisms lacking cytochrome c as part of their respiratory chain do not oxidize the reagent, leaving it colorless within the time limits of the test, and are oxidase negative slides BD DrySlide - PYR Kit BD DrySlide PYR is a disposable slide format for the presumptive identification of group A streptococci and enterococci. Contains the reagent L-pyrrolidonyl-ß-naphthylamide (PYR) which is hydrolyzed by bacteria possessing the enzyme pyrrolidonyl amino peptidase. The addition of the BD DrySlide PYR Color Developer, which is provided in convenient crush ampules, results in a vivid color reaction in a 3 minute test. 60 tests consisting of: 15 BD DrySlide PYR Slides 15 x 0.5 ml BD DrySlide PYR Color Developer 7-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

157 Identification & Susceptibility Testing BD Taxo Manual Differentiation BD Taxo Antibiotic Detection Discs x 50 discs x 50 discs BD Taxo - Blank Discs : Diameter ½". APHA, Standard Methods for the Examination of Dairy s : Diameter ¼", S&S-470. BD Taxo Anaerobe Differentiation Kit for Gram Negative Anaerobic Bacilli kit BD Taxo - Anaerobe Disc Set For Presumptive Identification of Gram-Negative Anaerobic Bacilli. These discs are recommended for use in the presumptive identification of gram-negative anaerobic bacilli based on differences in susceptibility to antimicrobial agents. This procedure is not for use for therapeutic purposes. These ¼" discs are made from high quality absorbent paper impregnated with accurately determined amounts of antimicrobial agents. They are furnished in cartridges containing 50 discs each. The last disc in each cartridge is marked "X" and contains the drug as coded. Cartridges are for use in single disc dispensers. One set of 5 cartridges Set components - one cartridge of 50 discs of each of the following antimicrobial agents: BD Taxo Kanamycin 1 mg K, BD Taxo Rifampin 15 μg RA, BD Sensi-Disc Penicillin 2 units P-2, BD Sensi-Disc Vancomycin 5 μg Va-5, BD Sensi-Disc Colistin 10 μg CL-10. BD Diagnostics Diagnostic Systems Catalog 2008/

158 Identification & Susceptibility Testing BD Taxo Manual Differentiation BD Taxo Carbohydrate Discs 50 discs per cartridge discs discs x 50 discs discs x 50 discs discs x 50 discs discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs discs x 50 discs BD Taxo - Adonitol Discs BD Taxo - Arabinose Discs BD Taxo - Dextrose Discs BD Taxo - Dulcitol Discs BD Taxo - Galactose Discs BD Taxo - Inositol Discs BD Taxo - Lactose Discs BD Taxo - Maltose Discs BD Taxo - Mannitol Discs BD Taxo - Raffinose Discs BD Taxo - Rhamnose Discs BD Taxo - Sorbitol Discs BD Taxo - Sucrose Discs BD Taxo - Trehalose Discs 7-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

159 Identification & Susceptibility Testing BD Taxo Manual Differentiation BD Taxo Differentiation Discs & Strips discs g mg discs x 50 discs x 50 discs discs discs x 50 discs discs discs x 50 discs BD Cefinase Paper Disc (¼") Paper Discs for the Detection of Beta-Lactamase Enzymes. Impregnated with Nitrocefin (see below). Used in rapid testing of isolated colonies of Neisseria gonorrhoeae, Staphylococcus spp., Haemophilus influenzae and anaerobic bacteria for the production of beta-lactamase. Nitrocefin Powder BD Taxo - A Discs BD Taxo A discs are for the presumptive identification of group A beta-hemolytic streptococci based on susceptibility to a low level of bacitracin. Discs are intended for use with pure cultures : Single vial 50 discs : Pkg. of 6 vials : Pkg. of 10 cartridges BD Taxo - ALA Discs BD Taxo Differentiation Discs ALA (delta-aminolevulinic acid) are used for rapidly detecting porphyrin as a means of speciating Haemophilus species. These are paper discs that contain buffered delta-aminolevulinic acid. BD Taxo - Hippurate Discs BD Taxo Differentiation Discs (¼") Hippurate are used for detecting the hydrolysis of sodium hippurate by betahemolytic group B streptococci, as well as by other organisms. The paper discs are containing sufficient sodium hippurate to yield a positive reaction with organisms producing sufficient hippuricase to hydrolyze the substrate. BD Taxo - Kanamycin Discs For differentiating gram-negative anaerobic bacilli. BD Taxo 1 mg Discs, Single cartridge of 50 discs. BD Taxo - N Discs BD Taxo N discs are used in qualitative procedures to distinguish those organisms which produce oxidase. These discs may be used for the presumptive differentiation of the genus Neisseria from other gram-negative cocci and members of the genus Pseudomonas from enteric bacilli : Single vial of 50 discs : Pkg. of 6 vials discs discs BD Taxo - Nitrate Discs BD Taxo Differentiation Discs Nitrate are used for detecting nitrate reductase activity by anaerobes in the nitrate reduction test. These are paper discs imprinted with the letters NIT and impregnated with a solution containing 40 % potassium nitrate and 0.1 % sodium molybdate. Round, off-white (ivory) 1/4" paper discs with "NIT" printed on both sides. BD Taxo - Novobiocin Discs BD Taxo Differentiation Discs Novobiocin are recommended for the differentiation of coagulasenegative staphylococci (e.g., Staphylococcus saprophyticus) based on novobiocin resistance. These are paper discs containing 5 μg novobiocin. BD Diagnostics Diagnostic Systems Catalog 2008/

160 Identification & Susceptibility Testing BD Taxo Manual Differentiation BD Taxo Differentiation Discs & Strips discs x 50 discs BD Taxo - ONPG Discs Used for the detection of lactose fermenters, especially those that do not promptly ferment lactose in some routine identification media such as Triple Sugar Iron Agar (TSI Agar) or Kligler Iron Agar : Single vial of 50 discs : Pkg. of 6 vials discs x 50 discs discs x 50 discs BD Taxo - P Discs BD Taxo P Discs are impregnated with ethylhydrocupreine hydrochloride (optochin), a drug used for pneumonia therapy before sulfonamides became available. The growth of pneumococci, but not of other streptococci, is markedly inhibited by this chemical. Pneumococci may, therefore, be differentiated from other alpha-hemolytic streptococci by the formation of a zone of inhibition around a BD Taxo P disc placed on a blood agar plate heavily inoculated with a pure culture suspected to be Streptococcus pneumoniae : Single vial of 50 discs : Pkg. of 6 vials : Single cartridge of 50 discs : Pkg. of 10 cartridges discs x 50 discs x 50 discs x 50 discs x 50 discs strips x 50 strips BD Taxo - SPS Discs BD Taxo Differentiation Discs SPS (sodium polyanethol sulfonate) are used for presumptively identifying Peptostreptococcus anaerobius and Gardnerella vaginalis. These are paper discs that contain sodium polyanethol sulfonate. BD Taxo - Urea Discs BD Taxo Differentiation Discs Urea are paper discs containing urea for differentiating microorganisms based on urease production. These are ¼" yellowish to slightly orange paper discs. The discs contain a sufficient amount of Urea R Broth to yield a positive reaction with organisms producing sufficient urease to hydrolyze the substrate. BD Taxo - V Factor Discs Diameter ¼". For differentiating Haemophilus species. Round, white ¼" paper discs with "V" printed on both sides. BD Taxo - X and V Factor Discs Diamter ¼". For differentiating Haemophilus species. Round, light tan ¼" paper discs with "VX" printed on both sides. BD Taxo - X Factor Discs Diamter ¼". For differentiating Haemophilus species. Round, brown ¼" paper discs with "X" printed on both sides. BD Taxo - V Factor Strips BD Taxo Strips impregnated with X, V and X and V factors are used in a qualitative procedure for the isolation and differentiation of Haemophilus species. The growth of these organisms is partially dependent upon the presence of the X or V factor or both. These strips provide a simple method for determining the growth requirements of organisms in this group. BD Taxo X and V Factor Strips may be substituted for the Staphylococcus streak used in some laboratories for isolation of Haemophilus when the base medium is BD Trypticase Soy Agar with or without sheep blood : 1 vial of 50 strips : Pkg. of 6 Vials BD Diagnostics Diagnostic Systems Catalog 2008/2009

161 Identification & Susceptibility Testing BD Taxo Manual Differentiation BD Taxo Differentiation Discs & Strips strips x 50 strips strips x 50 strips BD Taxo - X and V Factor Strips For differentiating Haemophilus species : 1 vial of 50 strips : Pkg. of 6 Vials. BD Taxo - X Factor Strips For differentiating Haemophilus species : 1 vial of 50 strips : Pkg. of 6 Vials. BD Diagnostics Diagnostic Systems Catalog 2008/

162 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc Dispensers BD Sensi-Disc - 6-Place Dispenser These BD Sensi-Disc self-tamping dispensers accommodate 6 cartridges of BD Sensi-Disc susceptibility test discs or BD Taxo anaerobe differentiation discs. They are used to deliver discs onto 100 mm-style Petri dishes when performing disc susceptibility testing or for the presumptive identification of gram-negative anaerobic bacilli. BD Sensi-Disc - 8-Place Dispenser These BD Sensi-Disc self-tamping dispensers accommodate 8 cartridges of BD Sensi-Disc susceptibility test discs or BD Taxo anaerobe differentiation discs. They are used to deliver discs onto 100 mm-style Petri dishes when performing disc susceptibility testing or for the presumptive identification of gram-negative anaerobic bacilli. BD Sensi-Disc - Designer Dispenser System The BD Sensi-Disc Designer Dispenser accommodates up to twelve cartridges of BD Sensi- Disc susceptibility test discs. It is used to deliver discs onto 150 mm-style Petri dishes when performing disc susceptibility testing, especially by the standardized method. BD Sensi-Disc - Single Disc Dispenser The BD Sensi-Disc Single Disc Dispenser is recommended for the individual application of cartridged brand antimicrobial susceptibility test and biochemical differentiation discs onto the surface of inoculated plated media. The Single Disc Dispenser replaces the manual placement of discs using sterile forceps BD Diagnostics Diagnostic Systems Catalog 2008/2009

163 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs BD Sensi-Disc - Amdinocillin, 10 μg (AMD-10) BD Sensi-Disc - Amikacin, 30 μg (AN-30) BD Sensi-Disc - Amoxicillin, 10 μg (AMX-10) BD Sensi-Disc - Amoxicillin, 25 μg (AMX-25) BD Sensi-Disc - Ampicillin, 2μg (AM-2) BD Sensi-Disc - Ampicillin, 10 μg (AM-10) BD Sensi-Disc - Ampicillin, 25 μg (AM-25) BD Sensi-Disc - Ampicillin/Sulbactam (SAM-20) Ampicillin 10 μg with Sulbactam 20 μg. BD Sensi-Disc - Augmentin (AMC-3) Amoxicillin 2 μg with Clavulanic Acid 1 μg. BD Sensi-Disc - Augmentin (AMC-30) Amoxicillin 20 μg with Clavulanic Acid 10 μg. BD Sensi-Disc - Azithromycin, 15 μg (AZM-15) BD Sensi-Disc - Azlocillin, 30 μg (AZ-30) Minimum order quantity: 1500 cartridges BD Sensi-Disc - Azlocillin, 75 μg (AZ-75) BD Sensi-Disc - Aztreonam, 30 μg (ATM-30) BD Sensi-Disc - Bacitracin, 2 μg (B-2) BD Sensi-Disc - Bacitracin, 10 μg (B-10) BD Sensi-Disc - Carbenicillin, 100 μg (CB-100) BD Sensi-Disc - Cefaclor, 30 μg (CEC-30) BD Sensi-Disc - Cefamandole, 30 μg (MA-30) BD Sensi-Disc - Cefazolin, 30 μg (CZ-30) BD Sensi-Disc - Cefdinir, 5 μg (CDR-5) BD Sensi-Disc - Cefepime, 30 μg (FEP-30) BD Diagnostics Diagnostic Systems Catalog 2008/

164 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs BD Sensi-Disc - Cefixime, 5 μg (CFM-5) BD Sensi-Disc - Cefixime, 10 μg (CFM-10) BD Sensi-Disc - Cefonicid, 30 μg (CID-30) BD Sensi-Disc - Cefoperazone, 75 μg (CFP-75) BD Sensi-Disc - Cefotaxime 30 μg with Clavulanic Acid 10 μg (CTX-30/CLA-10) ESBL Disc. BD Sensi-Disc - Cefotaxime, 5 μg (CTX-5) BD Sensi-Disc - Cefotaxime, 30 μg (CTX-30) ESBL Disc. BD Sensi-Disc - Cefotetan, 30 μg (CTT-30) BD Sensi-Disc - Cefoxitin, 30 μg (FOX-30) BD Sensi-Disc - Cefpirome, 30 μg (CPO-30) BD Sensi-Disc - Cefpodoxime, 10 μg (CPD-10) BD Sensi-Disc - Cefprozil, 30 μg (CPR-30) BD Sensi-Disc - Ceftazidime 30 μg with Clavulanic Acid 20 μg (CAZ-30/CLA-10) ESBL Disc. BD Sensi-Disc - Ceftazidime, 30 μg (CAZ-30) ESBL Disc. BD Sensi-Disc - Ceftizoxime, 30 μg (ZOX-30) BD Sensi-Disc - Ceftriaxone, 30 μg (CRO-30) BD Sensi-Disc - Cefuroxime, 5 μg (CXM-5) BD Sensi-Disc - Cefuroxime, 30 μg (CXM-30) BD Sensi-Disc - Cephalexin, 30 μg (CN-30) BD Sensi-Disc - Cephalothin, 30 μg (CF-30) BD Sensi-Disc - Cephradine, 30 μg (CH-30) BD Sensi-Disc - Chloramphenicol, 10 μg (C-10) 7-14 BD Diagnostics Diagnostic Systems Catalog 2008/2009

165 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs Discs x 50 discs discs x 50 discs discs BD Sensi-Disc - Chloramphenicol, 30 μg (C-30) BD Sensi-Disc - Cinoxacin, 100 μg (CIN-100) BD Sensi-Disc - Ciprofloxacin, 1 μg (CIP-1) BD Sensi-Disc - Ciprofloxacin, 5 μg (CIP-5) BD Sensi-Disc - Clarithromycin, 2 μg (CLR-2) BD Sensi-Disc - Clarithromycin, 15 μg (CLR-15) BD Sensi-Disc - Clindamycin, 2 μg (CC-2) BD Sensi-Disc - Cloxacillin, 1 μg (CX-1) BD Sensi-Disc - Colistin, 10 μg (CL-10) BD Sensi-Disc - Colistin, 25 μg (CL-25) BD Sensi-Disc - Dalfopristin/Quinupristin (Synercid), 15 μg (SYN-15) BD Sensi-Disc - Doxycycline, 30 μg (D-30) BD Sensi-Disc - Enoxacin, 10 μg (ENX-10) BD Sensi-Disc - Ertapenem, 10 μg (ETP-10) BD Sensi-Disc - Erythromycin, 2 μg (E-2) BD Sensi-Disc - Erythromycin, 5 μg (E-5) BD Sensi-Disc - Erythromycin, 10 μg (E-10) BD Sensi-Disc - Erythromycin, 15 μg (E-15) BD Sensi-Disc - Fosfomycin, 200 μg (FOS-200) BD Sensi-Disc - Fosfomycin (FF-50) W/GL. BD Sensi-Disc - Furazolidone, 100 μg (FX-100) BD Sensi-Disc - Fusidic Acid, 10 μg (FA-10) BD Sensi-Disc - Gatifloxacin, 5 μg (GAT-5) BD Diagnostics Diagnostic Systems Catalog 2008/

166 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs x 50 discs discs x 50 discs x 50 discs discs discs x 50 discs x 50 discs discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs BD Sensi-Disc - Gentamicin, 10 μg (GM-10) BD Sensi-Disc - Gentamicin, 120 μg (GM-120) BD Sensi-Disc - Imipenem, 10 μg (IPM-10) BD Sensi-Disc - Kanamycin, 30 μg (K-30) BD Sensi-Disc - Levofloxacin, 1 μg (LVL-1) BD Sensi-Disc - Levofloxacin, 5 μg (LVL-5) BD Sensi-Disc - Lincomycin, 2 μg (L-2) BD Sensi-Disc - Linezolid, 10 μg (LZD-10) BD Sensi-Disc - Linezolid, 30 μg (LZD-30) Minimum order quantity: 1500 cartridges BD Sensi-Disc - Lomefloxacin, 10 μg (LOM-10) BD Sensi-Disc - Loracarbef, 30 μg (LOR-30) BD Sensi-Disc - Mecillinam, 10 μg (MEC-10) BD Sensi-Disc - Meropenem, 10 μg (MEM-10) BD Sensi-Disc - Metronidazole, 5 μg (MET-5) BD Sensi-Disc - Metronidazole Elution Discs BD Sensi-Disc Metronidazole Elution Discs, 80 μg, are to be used for determining the in vitro susceptibility of anaerobic bacteria to metronidazole by the broth-disc method. BD Sensi-Disc brand discs are prepared by impregnating high quality absorbent paper with accurately determined amounts of antimicrobial agents. Discs are clearly marked on both sides with letters and numbers designating the agent and the drug content. BD Sensi-Disc agents are furnished in cartridges containing 50 discs each. The last disc in each cartridge is marked "X" and contains the drug as coded. The cartridges are for use in Single Disc Dispensers. Single cartridge of 50 discs. BD Sensi-Disc - Mezlocillin, 30 μg (MZ-30) BD Sensi-Disc - Mezlocillin, 75 μg (MZ-75) BD Sensi-Disc - Minocycline, 30 μg (MI-30) BD Sensi-Disc - Moxalactam, 30 μg (MOX-30) 7-16 BD Diagnostics Diagnostic Systems Catalog 2008/2009

167 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs BD Sensi-Disc - Moxifloxacin, 5 μg (MXF-5) BD Sensi-Disc - Mupirocin, 5 μg (MUP-5) BD Sensi-Disc - Mupirocin, 200 μg (MUP-200) BD Sensi-Disc - Nafcillin, 1 μg (NF-1) BD Sensi-Disc - Nalidixic Acid, 30 μg (NA-30) BD Sensi-Disc - Neomycin, 5 μg (N-5) BD Sensi-Disc - Neomycin, 30 μg (N-30) BD Sensi-Disc - Netilmicin, 10 μg (NET-10) BD Sensi-Disc - Netilmicin, 30 μg (NET-30) BD Sensi-Disc - Nitrofurantoin, 50 μg (FM-50) BD Sensi-Disc - Nitrofurantoin, 100 μg (FM-100) BD Sensi-Disc - Nitrofurantoin, 200 μg (FM-200) BD Sensi-Disc - Nitrofurantoin, 300 μg (FM-300) BD Sensi-Disc - Norfloxacin, 2 μg (NOR-2) BD Sensi-Disc - Norfloxacin, 5 μg (NOR-5) Minimum order quantity: 1500 cartridges BD Sensi-Disc - Norfloxacin, 10 μg (NOR-10) BD Sensi-Disc - Novobiocin, 5 μg (NB-5) BD Sensi-Disc - Novobiocin, 30 μg (NB-30) BD Sensi-Disc - Ofloxacin, 5 μg (OFX-5) BD Sensi-Disc - Oleandomycin, 15 μg (OL-15) BD Sensi-Disc - Oxacillin, 1 μg (OX-1) BD Sensi-Disc - Oxolinic Acid, 2 μg (OA-2) BD Sensi-Disc - Oxytetracycline, 30 μg (T-30) BD Diagnostics Diagnostic Systems Catalog 2008/

168 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs x 50 discs x 50 discs discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs discs discs x 50 discs x 50 discs BD Sensi-Disc - Pefloxacin, 5 μg (PEF-5) BD Sensi-Disc - Penicillin, 1 μg (P-1) BD Sensi-Disc - Penicillin, 1.5 μg (P-1.5) BD Sensi-Disc - Penicillin, 2 μg (P-2) BD Sensi-Disc - Penicillin, 10 μg (P-10) BD Sensi-Disc - Piperacillin, 75 μg (PIP-75) BD Sensi-Disc - Piperacillin, 100 μg (PIP-100) BD Sensi-Disc - Piperacillin 75 μg + Tazobactam10 μg (PIP-75/TAZ-10) BD Sensi-Disc - Piperacillin 100 μg + Tazobactam 10 μg (PIP-100/TAZ-10) BD Sensi-Disc - Polymyxin B, 300 μg (PB-300) BD Sensi-Disc - Quinupristin/Dalfopristin (Synercid) 15 μg (SYN-15) BD Sensi-Disc - Rifampicin, 2 μg (RA-2) BD Sensi-Disc - Rifampicin, 30 μg (RA-30) BD Sensi-Disc - Rokitamycin, 30 μg (ROK-30) BD Sensi-Disc - Spectinomycin, 100 μg (SPT-100) BD Sensi-Disc - Spiramicin, 100 μg (SP-100) BD Sensi-Disc - Sulfadiazine, 0.25 μg (SD-0.25) BD Sensi-Disc - Sulfathiazole, 0.25 μg (ST-0.25) BD Sensi-Disc - Sulfisoxazole, 25 μg (G-25) BD Sensi-Disc - Teicoplanin, 30 μg (TEC-30) BD Sensi-Disc - Telithromycin, 15 μg (TEL-15) BD Sensi-Disc - Temocillin, 30 μg (TEM-30) BD Sensi-Disc - Tetracycline, 5 μg (TE-5) BD Sensi-Disc - Tetracycline, 10 μg (TE-10) 7-18 BD Diagnostics Diagnostic Systems Catalog 2008/2009

169 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in Blister Packs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs BD Sensi-Disc - Tetracycline, 30 μg (TE-30) BD Sensi-Disc - Ticarcillin, 75 μg (TIC-75) BD Sensi-Disc - Ticarcillin with Clavulanic Acid (Timentin), 85 μg (TIM-85) BD Sensi-Disc - Tobramycin, 10 μg (NN-10) BD Sensi-Disc - Trimethoprim, 5 μg (TMP-5) BD Sensi-Disc - Trimethoprim 1.25 μg + Sulfamethoxazol μg (Cotrimoxazol) (SXT) x 50 discs x 50 discs x 50 discs x 50 discs x 50 discs BD Sensi-Disc - Trimethoprim, 1.25 μg (TR-1.25) BD Sensi-Disc - Trimethoprim, 2.5 μg (TR-2.5) BD Sensi-Disc - Triple Sulfa, 25 μg (SSS-25) Each 1/3 of Sulfamethazin, Sulfamerazin and Sulfadiazin. BD Sensi-Disc - Vancomycin, 5 μg (VA-5) BD Sensi-Disc - Vancomycin, 30 μg (VA-30) BD Diagnostics Diagnostic Systems Catalog 2008/

170 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in a Glass Vial discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs BD Sensi-Disc - Amikacin, 10 μg (AN-10) BD Sensi-Disc - Amikacin, 30 μg (AN-30) BD Sensi-Disc - Amoxicillin, 25 μg (AMX-25) BD Sensi-Disc - Ampicillin, 10 μg (AM-10) BD Sensi-Disc - Ampicillin, 25 μg (AM-25) BD Sensi-Disc - Augmentin (AMC-30) Amoxicillin 20 μg with Clavulanic Acid 10 μg. BD Sensi-Disc - Azlocillin, 30 μg (AZ-30) BD Sensi-Disc - Bacitracin, 10 μg (B-10) BD Sensi-Disc - Blank Disc Nonsterile BD Sensi-Disc - Cefaclor, 30 μg (CEC-30) BD Sensi-Disc - Cefadroxyl, 30 μg (CFR-30) BD Sensi-Disc - Cefazolin, 30 μg (CZ-30) BD Sensi-Disc - Cefepime, 30 μg (FEP-30) BD Sensi-Disc - Cefoperazone, 30 μg (CFP-30) BD Sensi-Disc - Cefotaxime, 30 μg (CTX-30) BD Sensi-Disc - Cefotiam, 30 μg (CFT-30) BD Sensi-Disc - Cefoxitin, 30 μg (FOX-30) BD Sensi-Disc - Cefsulodin, 30 μg (CFS-30) BD Sensi-Disc - Ceftazidime, 30 μg (CAZ-30) BD Sensi-Disc - Ceftriaxone, 30 μg (CRO-30) BD Sensi-Disc - Cefuroxime, 30 μg (CXM-30) BD Sensi-Disc - Cephalexin, 30 μg (CN-30) BD Sensi-Disc - Cephalotin, 30 μg (CF-30) BD Sensi-Disc - Ciprofloxacin, 5 μg (CIP-5) 7-20 BD Diagnostics Diagnostic Systems Catalog 2008/2009

171 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in a Glass Vial discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs BD Sensi-Disc - Clindamycin, 2 μg (CC-2) BD Sensi-Disc - Clindamycin, 10 μg (CC-10) BD Sensi-Disc - Colistin, 10 μg (CL-10) BD Sensi-Disc - Doxycycline, 30 μg (D-30) BD Sensi-Disc - Erythromycin, 15 μg (E-15) BD Sensi-Disc - Fosfomycin, 50 μg (FF-50) BD Sensi-Disc - Fusidic Acid, 10 μg (FA-10) BD Sensi-Disc - Gentamicin, 10 μg (GM-10) BD Sensi-Disc - Imipenem, 10 μg (IPM-10) BD Sensi-Disc - Kanamycin, 30 μg (K-30) BD Sensi-Disc - Lincomycin, 15 μg (L-15) BD Sensi-Disc - Meropenem, 10 μg (MEM-10) BD Sensi-Disc - Metronidazole, 80 μg (MET-80) BD Sensi-Disc - Mezlocillin, 30 μg (MZ-30) BD Sensi-Disc - Nalidixic Acid, 30 μg (NA-30) BD Sensi-Disc - Neomycin, 30 μg (N-30) BD Sensi-Disc - Netilmicin, 30 μg (NET-30) BD Sensi-Disc - Nitrofurantoin, 300 μg (FM-300) BD Sensi-Disc - Nitrofurantoin 300 μg + Sulfafiazine 100 μg (FM-300/SD-100) BD Sensi-Disc - Norfloxacin, 10 μg (NOR-10) BD Sensi-Disc - Novobiocin, 5 μg (NB-5) BD Sensi-Disc - Ofloxacin, 5 μg (OFX-5) BD Sensi-Disc - Ofloxacin, 10 μg (OFX-10) BD Sensi-Disc - Oleandomycin, 15 μg (OL-15) BD Diagnostics Diagnostic Systems Catalog 2008/

172 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc in a Glass Vial discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs BD Sensi-Disc - Oxacillin, 1 μg (OX-1) BD Sensi-Disc - Oxacillin, 5 μg (OX-5) BD Sensi-Disc - Penicillin, 10 μg (P-10) BD Sensi-Disc - Phenicol, 30 μg (C-30) BD Sensi-Disc - Pipemidic Acid, 20 μg (PI-20) BD Sensi-Disc - Piperacillin, 30 μg (PIP-30) BD Sensi-Disc - Piperacillin, 100 μg (PIP-100) BD Sensi-Disc - Polymyxin B, 300 μg (PB-300) BD Sensi-Disc - Sulbactam 10 μg with Ampicillin 20 μg (SAM-30) BD Sensi-Disc - Sulbactam 30 μg with Cefoperazone 75 μg (SCP 105) For Pharma Customers only - minimum order quantity cartridges BD Sensi-Disc - Sulfamethoxazole, 23,75 μg (SMZ-23,75) BD Sensi-Disc - Tetracycline, 30 μg (TE-30) BD Sensi-Disc - Tobramycin, 10 μg (NN-10) BD Sensi-Disc - Tobramycin, 30 μg (NN-30) BD Sensi-Disc - Trimethoprim 1.25 μg + Sulfamethoxazol μg (Cotrimoxazol) (SXT) discs discs BD Sensi-Disc - Trimethoprim, 5 μg (TMP-5) BD Sensi-Disc - Vancomycin, 30 μg (VA-30) 7-22 BD Diagnostics Diagnostic Systems Catalog 2008/2009

173 Identification & Susceptibility Testing BD Sensi-Disc Manual Susceptibility Testing BD Sensi-Disc Antimycobacterial Discs for Use in Culture Media discs discs discs discs discs discs x 50 discs x 50 discs discs discs BD Sensi-Disc - Ethambutol, 25 μg (EM-25) BD Sensi-Disc - Ethambutol, 50 μg (EM-50) BD Sensi-Disc - Ethionamide, 25 μg (EA-25) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 1 μg (INH-1) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 5 μg (INH-5) BD Sensi-Disc - Rifampin, 25 μg (RA-25) BD Sensi-Disc - Rifampin, 5 μg (RA-5) BD Sensi-Disc - Streptomycin, 10 μg (S-10) BD Sensi-Disc - Streptomycin, 50 μg (S-50) BD Sensi-Disc - Streptomycin, 300 μg (S-300) BD Sensi-Disc for Veterinary Laboratory Use discs discs BD Sensi-Disc - Ceftiofur, 30 μg (XNL-30) BD Sensi-Disc - Enrofloxacin, 5 μg (ENO-5) Accessories for Manual Susceptibility Testing tubes McFarland Turbidity McFarland standards are used as turbidity standards in the preparation of suspensions of microorganisms. The McFarland 0.5 standard has particular application in the preparation of bacterial inocula for performing antimicrobial susceptibility testing. The performance of susceptibility testing requires the use of standard inocula. The McFarland 0.5 standard is used for the preparation of inocula in standardized agar dilution, broth macro- and microdilution, disc diffusion and anaerobic organism susceptibility test procedures. Contains 10 Size K Tubes. BD Diagnostics Diagnostic Systems Catalog 2008/

174 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Instrument & Accessories BD Phoenix Instrument The BD Phoenix Automated Microbiology System is designed for the rapid Identification (ID) and Antimicrobial Susceptibility Testing (AST) of clinically significant human bacterial pathogens. The instrument holds up to 100 panels (ID only, AST only, combined ID/AST, or a mixture), thus being capable of performing 200 simultaneous tests with the combined ID/AST panels. Identification is based on colorimetric and fluorescence readings without the need for supplemental tests or reagent addition. The AST is based on a dual indicator system (redox and turbidity readings) for optimal performance. Panel readings are taken by the BD Phoenix instrument every 20 minutes and on average, provide the final interpretation of ID results in less than 3 hours and of AST results in approximately 8 hours. Over 160 Gram-negative organisms and over 145 Gram-positive organisms are in the BD Phoenix database. The BD Phoenix supports CLSI (formerly NCCLS), SFM, DIN, and BSAC based rules as well as customizable breakpoints. An integrated customizable expert system (BDXpert System) is used for result validation. The BD Phoenix System features only one moving part, easy-to-use icon driven software, rapid reporting of preliminary results, specimen barcoding, direct LIS connection and minimal user maintenance. The System can be connected to the BD EpiCenter Data Management System which provides the laboratory with workflow management, patient demographic storage, advanced query and custom reporting, and infection control and epidemiology analysis BD Phoenix - Starter Kit The BD Phoenix Starter Kit includes: 5 inoculation stations, 2 panel caddies, 1 fixed volume pipette, 960 pipette tips, 1 temperature panel, 4 spare air filters, 1 external bar code scanner, 3 power cords and 1 quick reference guide BD Phoenix Table BD Phoenix - 25 μl Hamilton-Pipette The BD Phoenix 25 μl Hamilton-Pipette is used for the transfer of bacterial suspensions from the inoculated ID Broth tubes to the AST broth tubes in the 0.5 McFarland Mode. BD Phoenix - 50 l Hamilton-Pipette The BD Phoenix 25 μl Hamilton-Pipette is used for the transfer of bacterial suspensions from the inoculated ID Broth tubes to the AST broth tubes in the 0.25 McFarland Mode. BD Phoenix - Inoculation Station The BD Phoenix Inoculation Station is designed to hold six tubes of broth (3 ID, 3 AST) and three panels at an angle of 24 in order to provide the proper gravity-driven inoculum flow of broth through panels during panel inoculation. BD Phoenix - Panel Caddy The BD Phoenix Panel Caddy is a molded plastic tray used to transport filled and sealed panels from the preparation bench to the BD Phoenix instrument. Each caddy can carry up to 20 panels BD Diagnostics Diagnostic Systems Catalog 2008/2009

175 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Instrument & Accessories BD Phoenix - Panel Closure 100 panel closures, used for sealing of BD Phoenix panels after inoculation. BD Phoenix - Pipette Stand BD Phoenix - Pipette Tips Extra long, aerosol-resistant pipette tips for use with the BD Phoenix 25 μl Hamilton-Pipette ( ). Packaged as 204 tips per rack; 8 racks per shelf pack (in total 1632 pipette tips). Cat.No replaces former Cat.No BD PhoenixSpec - Nephelometer A battery-powered, portable device designed for measuring inoculum density in McFarland units. The device is accurate from McFarland units in increments of 0.1 units. The instrument can be used for any laboratory procedure that requires inoculum density adjustments within this range. Used with BD PhoenixSpec Calibrator Kit, x 4.5 ml BD PhoenixSpec - Calibration Standards One set contains a BD Phoenix calibration standard (0.25 / 0.5 / 1.0 / 4.0 McFarland). Used for the proper calibration of a BD Phoenix Nephelometer. BD Phoenix - ID Broth The BD Phoenix ID broth is a modified saline solution that, after addition of bacteria, is used for identification of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 4.5 ml. 1 tube of ID Broth is sufficient to inoculate 1 BD Phoenix panel x 2.2 ml BD Phoenix - Low Volume Broth The BD Phoenix low volume broth is used in case of sparse colony growth in order to reach either a 0.25 or 0.5 McFarland, respectively. In case of an 0.25 McFarland adjustment, 50 μl of ID broth must be transferred to an AST broth instead of 25 μl (this procedure can only be done by using the BD PhoenixSpec Cat.No ). The low volume broth is a modified saline solution that, after addition of bacteria, is used only for susceptibility testing of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 2.2 ml. 1 tube of low volume broth is sufficient to inoculate 1 BD Phoenix panel x 8 ml BD Phoenix - AST Broth The BD Phoenix AST broth is modified Mueller Hinton Broth that, after the addition of AST indicator and bacterial suspension, is used for susceptibility testing of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 8.0 ml. 1 tube of AST Broth is sufficient to inoculate 1 BD Phoenix panel. BD Diagnostics Diagnostic Systems Catalog 2008/

176 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Instrument & Accessories x 6 ml BD Phoenix - AST Indicator The BD Phoenix AST indicator is a redox indicator that, after addition to AST broth, is used for susceptibility testing of microorganisms by the BD Phoenix system. Each 6 ml bottle of AST Indicator is sufficient for the inoculation of 100 AST Broth tubes, i.e. 1 package (10 bottles) is sufficient for 1000 tubes BD Diagnostics Diagnostic Systems Catalog 2008/2009

177 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Gram Positive Combo Panels The BD Phoenix Gram Positive Combo Panels are used for the identification and susceptibility testing of Gram-positive bacteria with selected antimicrobial agents. Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic BD Phoenix - Gram Positive Combo Panel (PMIC/ID-51) Special Gram Positive Combo Panel for the Turkish market. List of Antimicrobials (μg/ml): AXC 2-8, AM 2-8, CZ 2-8, FOX 2-8, CIP 0.5-2, CC 0.5-4, E , FF 16-64, FA 2-8, GM 1-8, GMS 500 -, LVX 0.5-2, LZD 1-4, MEM 2-8, MXF 0.5-2, MUH -256, OFX 0.5-2, OX , P , RA 1-4, STS , TEC 1-16, TE 0.5-4, STG , SXT 0.5-2, VA BD Phoenix - Gram Positive Combo Panel (PMIC/ID-53) List of Antimicrobials (μg/ml): AMC 1-4, AM , CZ 2-16, FOX 2-8, C 1-16, CIP 0.5-2, CC 0.5-2, E , FF 16-64, FA 2-8, GM 2-8, GMS 500 -, LVX 1-4, LZD 1-4, MUH - 256, FM 16-64, OX , P , SYN 0.5-2, RA 0.5-2, STS , TEC 1-16, SXT 0.5-2, VA BD Phoenix - Gram Positive Combo Panel (PMIC/ID-55) List of Antimicrobials (μg/ml): AN 8-32, AMC 1-4, AM 2-8, FOX 2-8, CIP , CC 0.5-2, E , FA 1-8, GM 1-8, GMS 500 -, LZD 0.5-4, MUP 1-4, MUH - 256, FM 16-64, OX , P , SYN 0.5-2, RA 0.5-2, TEC 1-16, TE 0.5-8, NN 2-8, TMP 0.5-2, SXT 1-4, VA 1-16 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-57) Special Gram Positive Combo Panel for the Middle East market. List of Antimicrobials (μg/ml): MXF 1-4, AMC 1-4, MEM 2-8, GM 2-8, CRO 8-32, FOX 1-8, LZD 1-4, P 0,06-0,25, OX 0,25-2, GMS 500, FA 4-16, E 0,25-4, CC 0,5-2, CLR 0,25-4, CIP 0,5-2, CTX 8-32, AM 2-8, SYN 0,5-2, RA 0,5-2, TEC 1-16, TE 0,5-8, STG 1-4, VA BD Phoenix - Gram Positive Combo Panel (PMIC/ID-58) List of Antimicrobials (μg/ml): AMC 1-4 / 0.5-2, AM 1-8, FOX 2-8, CIP 0.5-2, CC 0.5-2, E , FF , FA 2-8, GM 2-8, GMS 500, LVX 1-4, LZD 1-4, MXF 0.5-2, MUP 1-4, MUH 256, FM 16-64, OX , P , SYN 0.5-2, RA 0.5-2, TEC 1-16, TE 0.5-8, TMP 2-8, SXT / , VA 1-16 BD Phoenix Staphylococci Panel (PMIC/ID-60) List of Antimicrobials(μg/ml): FOX 2-8, DAP 1-4, E , FF 16-64, FA 2-16GM 0.5-8, K 4-16, LVX 0.5-4, L 1-8, LZD 0.5-4, MOX 2-16, OX , P , PR , RA , TEC 1-32, TE 0.5-8, NN 1-4, SXT 2-8, VA 1-32 BD Phoenix - Enterococci Panel (PMIC/ID-61) List of Antimicrobials (μg/ml): AM , C 1-16, CIP 0.5-4, CC , DAP 1-4, E , GMS 500, LVX , L 1-8, LED 0.5-4, MXF 0.5-4, FM , PR , SYN 0.5-2, STS 1000, TEC , TE 0.5-8, SXT 2/38-8/152, VA BD Phoenix - Gram Positive Combo Panel (PMIC/ID-63) List of Antimicrobials (μg/ml): AM 2-16, AMC 1/0.5-16/8, C 1-16, CC , CIP 0.5-2, DAP 1-16, E , FA 1-8, FM 16-64, FOX 2-8, GM 1-4, GMS 500, LVX 0.5-2, LZD 2-8, MI 1-4, MUH 256, MUP 1-4, OX 1-4, P , RA , STS 1000, TE 0.5-2, TEC 1-16, VA 1-16 BD Diagnostics Diagnostic Systems Catalog 2008/

178 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Gram Positive Combo Panels The BD Phoenix Gram Positive Combo Panels are used for the identification and susceptibility testing of Gram-positive bacteria with selected antimicrobial agents. Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic BD Phoenix - Gram Positive Combo Panel (PMIC/ID-64) List of Antimicrobials (μg/ml): AM 2-8, FOX 2-16, CIP 0.5-2, CC 0.5-4, ETP 0.5-4, E , FF , FA 2-16, GM 1-8, GMS 500, IPM 2-8, LVX 0.5-4, LZD 1-4, MEM 2-8, MXF 0.5-2, MUH 256, OX , P , RA 0.5-2, STS 1000, TEC 1-16, TE 0.5-8, SXT 0.5-2, VA 1-16 BD Phoenix Gram Positive Combo Panel (PMIC/ID-65) List of Antimicrobials (μg/ml): AM , SAM 4/2-16/8, FOX 2-16, CIP 0.5-2, CC 0.5-2, E , FF , FA 4-16, GM 2-8, GMS 500, IPM 2-8, LVX 1-4, LZD 1-4, MEM 2-8, MXF 0.5-2, OX , P , RA 0.5-2, STS 1000, TEC 1-16, TE 0.5-8, NN 2-8, SXT 0.5/9.5-2/38, VA BD Diagnostics Diagnostic Systems Catalog 2008/2009

179 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Gram Negative Combo Panels The BD Phoenix Gram Negative Combo Panels are used for the identification and susceptibility testing of Gram-negative bacteria with selected antimicrobial agents. Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic BD Phoenix - Gram Negative Combo Panel (NMIC/ID-50) List of Antimicrobials (μg/ml): AN 4-32, AMC 4-16, AXC 2-8, AM 2-16, ATM 2-16, CZ 4-16, FEP 2-16, CTX 1-32, CAZ 4-16, CXM 4-16, CIP 0.5-2, ESR, GM 1-8, IPM 1-4, LVX 1-4, MEM 1-8, PIP 4-64, TZP 4-64, TE 1-8, NN 1-4, STG , SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-51) List of Antimicrobials (μg/ml): AN 2-32, AMC 4-16, AM 4-16, ATM 1-16, CZ 4-16, FEP 1-16, CAZ 1-16, CRO 2-32, CXM 2-16, CIP , CO 0.5-4, ESR, GM 2-8, LVX 1-4, MEM 1-8, FM 16-64, PIP 4-64, TZP 4-64, TEM 2-16, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-52) List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-16, ATM 2-16, CZ 4-16, CAZ 2-16, CRO 1-32, CXM 4-16, CIP , CO 0.5-2, ESR, GM 2-8, IPM 1-8, LVX 1-4, MEM 1-8, FM 16-64, NOR 2-8, PIP 4-64, TZP 4-64, NN 2-8, TMP 1-8, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-55) TURKEY Special Gram Negative Combo Panel for the Turkish market. List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, SCP , CTX 4-32, FOX 4-16, CAZ 1-16, C 4-16, CIP 0.5-2, CO 0.5-2, ESR, GM 2-8, IPM 1-8, LVX 1-4, MEM 1-8, PIP 4-64, TZP 4-64, TE 2-8, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-57) List of Antimicrobials (μg/ml): AN 4-32, AMC 2-16, AM 2-32, ATM 2-16, CZ 4-16, FEP 1-16, CTX 1-32, CAZ 1-16, CXM 4-16, CIP 0.5-4, CO 1-4, ESR, GM 1-8, MEM 1-8, NET 1-16, FM 16-64, NOR 2-8, TZP 4-64, TMP 0.5-4, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-63) MIDDLE EAST Special Gram Negative Combo Panel for the Middle East market. List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-32, Aztreonam 2-16, FEP 2-16, CTX 1-32, FOX 4-16, CAZ 1-16, CXM 4-16, CF 4-16, CIP 0,5-2, Colistin 0,5-2, GM 2-8, IPM 1-8, MEM 1-8, FM 16-64, NOR 2-8, PIP 4-64, TZP 4-64, TE 2-8, STG BD Phoenix - Gram Negative Combo Panel (NMIC/ID-64) List of Antimicrobials (μg/ml): AN 16-64, AM 4-16, SAM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CTX 4-32, CAZ 4-16, CXM 4-16, CIP 0.5-2, FF , GM 2-8, IPM 1-8, LVX 1-4, MEM 1-8, MXF 1-4, PIP 4-64, TZP 4-64, TE 2-8, NN 2-8, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-65) List of Antimicrobials (μg/ml): AN 4-32, AMC 2-32, AM 4-32, ATM 1-16, CTX 1-4, FOX 1-4, CAZ 2-16, CXM 4-16, CN 4-16, CIP , CO 1-4, ETP 0.5-4, GM 1-8, IPM 1-8, LVX 0.5-2, MEM 1-8, FM 16-64, TZP 4-32, NN 2-8, TMP 0.5-4, STG BD Diagnostics Diagnostic Systems Catalog 2008/

180 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Gram Negative Combo Panels The BD Phoenix Gram Negative Combo Panels are used for the identification and susceptibility testing of Gram-negative bacteria with selected antimicrobial agents. Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic BD Phoenix - Pseudomonas Panel (NMIC/ID-68) List of Antimicrobials (μg/ml): AN 8-32, ATM 1-32, FEP , CTX , CFS 1-32, CAZ 1-32, CIP 0.5-2, CO 0.5-2, FF 16-64, GM 2-8, IPM 0.5-8, MEM 1-8, PIP 4-64, TZP 4-64, TIC 1-64, TIM 8-64, NN 2-8, SXT 2-8, BD Phoenix - Gram Negative Combo Panel (NMIC/ID-69) List of Antimicrobials (μg/ml): AN 8-32, AXC 2-16, AM 4-16, ATM 1-32, FEP 1-32, CTX 1-32, FOX 4-32, CAZ 1-32, CF 1-32, CIP 0.5-2, GM 2-8, IPM 1-8, NOR 0.5-2, PIP 4-64, TZP 4-64, TIC 8-64, NN 2-8, SXT 2-8 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-71) Pseudomonas Panel. List of Antimicrobials (μg/ml): AN 4-32, FEP , CTX , CFS 4-32, CAZ , CIP , CO 0.5-2, FF 16-64, GM 1-8, IPM 1-8, LVX , MEM 1-8, MXF 0.5-4, PIP 4-64, TZP 4-64, TIC 8-64, NN 1-8, SXT BD Phoenix - Gram Negative Combo Panel (NMIC/ID-70) List of Antimicrobials (μg/ml): AMC 4-16, AM 4-16, SAM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CTX 2-32, FOX 1-16, CAZ 2-16, CXM 4-16, CIP 0.5-2, ETP 0.5-4, GM 2-8, LVX 1-4, MEM 1-8, MXF 1-4, PIP 4-64, TZP 4-64, TE 1-8, NN 2-8, SXT 0,5-2 BD Phoenix - Gram Negative Combo Panel (UNMIC/ID-53) Urine Panel List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CAZ 1-16, CRO 2-32, CXM 4-16, CIP 0.5-2, ESR, FF , GM 2-8, MEM 1-8, FM 16-64, NOR 2-8, PIP 4-64, TZP 4-64, TEM 4-16, TMP 1-8, SXT BD Phoenix - Gram Negative Combo Panel (UNMIC/ID-61) Urine Panel List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-16, ATM 2-16, CZ 4-16, CAZ 1-16, CRO 4-32, CXM 4-16, CIP , ESR, FF , GM 2-8, IPM 1-8, LVX 1-4, MEM 1-8, NA 4-16, FM 16-64, NOR 2-8, PIP 4-64, TZP 4-64, SXT BD Phoenix - Gram Negative Combo Panel (UNMIC/ID-62) Urine Panel List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, FOX 4-16, CAZ 1-16, CRO 2-32, CXM 4-16, CIP , ESR, FF , GM 2-8, IPM 1-8, MEM 1-8, FM 16-64, NOR 2-8, TZP 4-64, NN 2-8, SXT BD Diagnostics Diagnostic Systems Catalog 2008/2009

181 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Gram Negative Combo Panels The BD Phoenix Gram Negative Combo Panels are used for the identification and susceptibility testing of Gram-negative bacteria with selected antimicrobial agents. Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic BD Phoenix - Gram Negative Combo Panel (UNMIC/ID-67) Urine Panel List of Antimicrobials (μg/ml): AN 8-32, AMC 4-16, AXC 2-16, AM 4-16, ATM 1-32, CFM 0.5-2, FOX 2-32, CAZ 1-32, CRO 1-32, CF 1-32, CIP 0.5-2, FF , GM 2-8, IPM 1-8, NA 4-16, FM , NOR 0.5-8, SXT 2-8 BD Diagnostics Diagnostic Systems Catalog 2008/

182 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix Streptococci Panel Full doubling antimicrobic concentrations with minimum three concentrations per antibiotic to allow real MIC determinations BD Phoenix Streptococci Panel (SMIC/ID-9) List of Antimicrobials(μg/ml): AMX , FEP 0.5-2, CTX 0.5-4, C 2-8, CC , E , GMS , LVX 0.5-4, LZD 1-4, MEM , MXF , , PR , TEC 1-8, TEL , TE 0.5-8, STG 0.5-2, VA 0.5-8, BD Phoenix - Streptococci Panel (SMIC-10 PLUS) The new Phoenix Streptococci panel provides the following benefits to fulfil your needs: The AST only panel comprises 136 wells fully dedicated to susceptibility testing. More drugs which are fully dedicated to Streptococci. Full range of antibiotic concentrations for the majority of drugs covering wild-type concentrations (Eucast epidemiological cut-offs) and all European guidelines. Full range of antibiotic concentrations, providing you with a real MIC with no additonal tests. By preparing a 0.25 MCF inoculum in the low volume ID tube, only a quarter of the biomass normally used will be necessary. List of Antimicrobials (μg/ml): AMX , FEP , CTX , C 1-16, CC , E , DAP , GMS , KS , , LZD , MEM , MXF , P , PR , STS , TEC 1-32, TEL , TE , STG 0.5-8, VA x 8 ml x 5 ml x 4.5 ml BD Phoenix - AST-S Broth The BD Phoenix AST-S broth is modified Mueller Hinton Broth that, after the addition of AST-S indicator and bacterial suspension, is used for susceptibility testing of streptococci by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 8.0 ml. 1 tube of ASTS Broth is sufficient to inoculate 1 BD Phoenix panel. BD Phoenix - AST-S Indicator The BD Phoenix AST-S indicator is a redox indicator that, after addition to AST-S broth, is used for susceptibility testing of streptococci by the BD Phoenix system. Each 6 ml bottle of AST-S Indicator is sufficient for the inoculation of 100 AST-S Broth tubes, i.e. 1 package (10 bottles) is sufficient for 1000 tubes. BD Phoenix - ID Broth The BD Phoenix ID broth is a modified saline solution that, after addition of bacteria, is used for identification of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 4.5 ml. 1 tube of ID Broth is sufficient to inoculate 1 BD Phoenix panel x 2.2 ml BD Phoenix - Low Volume Broth The BD Phoenix low volume broth is used in case of sparse colony growth in order to reach either a 0.25 or 0.5 McFarland, respectively. In case of an 0.25 McFarland adjustment, 50 μl of ID broth must be transferred to an AST broth instead of 25 μl (this procedure can only be done by using the BD PhoenixSpec Cat.No ). The low volume broth is a modified saline solution that, after addition of bacteria, is used only for susceptibility testing of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 2.2 ml. 1 tube of low volume broth is sufficient to inoculate 1 BD Phoenix panel BD Diagnostics Diagnostic Systems Catalog 2008/2009

183 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix ID Only Panels x 4.5 ml BD Phoenix - Gram Negative ID Panels The BD Phoenix Gram Negative ID panel is used for the identification of Gram-negative bacteria. The panel consists of 51 wells, 46 of which are filled with dried biochemical substrates and 2 wells with reaction controls. No off-line tests or reagent additions are required for the identification. The BD Phoenix system is able to identify approximately 160 Gram-negative strains of clinically relevant bacteria. 25 panels and panel closures are included in each package. BD Phoenix - Gram Positive ID Panels The BD Phoenix Gram Positive ID panel is used for the identification of Gram-positive bacteria. The panel consists of 51 wells, 46 of which are filled with dried biochemical substrates and 2 wells with reaction controls. No off-line tests or reagent additions are required for the identification. The BD Phoenix system is able to identify approximately 145 Gram-positive strains of clinically relevant bacteria. 25 panels and panel closures are included in each package. BD Phoenix - ID Broth The BD Phoenix ID broth is a modified saline solution that, after addition of bacteria, is used for identification of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 4.5 ml. 1 tube of ID Broth is sufficient to inoculate 1 BD Phoenix panel. BD Diagnostics Diagnostic Systems Catalog 2008/

184 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix AST Only Panels BD Phoenix AST Only Panel (UNMIC-53) Urine AST Only Panel List of Antimicrobials(μg/ml): AN 8-32, AMC 4/2-16/8, AM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CAZ 1-16, CRO 2-32, CXM 4-16, CP 0.5-2, FF , GM 2-8, MEM 1-8, FM 16-64, NOR 2-8, PIP 4-64, TZP 4/4-64/4 - TEM 4-16, TMP 1-8, SXT 0.5/9.5-2/ BD Phoenix AST Only Panel (UNMIC-67) Urine AST Only Panel List of Antimicrobials(μg/ml): AN 8-32, AMC 4-16, AXC 2-16, AM 4-16, ATM 1-32, CFM 0.5-2, FOX 2-32, CAZ 1-32, CRO 1-32, CF 1-32, CIP 0.5-2, FF , GM 2-8, IPM 1-8, NA 4-16, FM , NOR 0.5-8, SXT BD Phoenix AST Only Panel (NMIC-64) List of Antimicrobials(μg/ml): AN 16-64, AM 4-16, SAM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CTX 4-32, CAZ 4-16, CXM 4-16, CIP 0.5-2, FF , GM 2-8, IPM 1-8, LVX 1-4, MEM 1-8, MXF 1-4, PIP 4-64, TZP 4-64, TE 2-8, NN 2-8, SXT BD Phoenix AST Only Panel (NMIC-70) List of Antimicrobials(μg/ml): AMC 4-16, AM 4-16, SAM 4-16, ATM 2-16, CZ 4-16, FEP 2-16, CTX 2-32, FOX 1-16, CAZ 2-16, CXM 4-16, CIP 0.5-2, ETP 0.5-4, GM 2-8, LVX 1-4, MEM 1-8, MXF 1-4, PIP 4-64, TZP 4-64, TE 1-8, NN 2-8, SXT 0,5-2 BD Phoenix AST Only Panel (NMIC-71) List of Antimicrobials(μg/ml): AN 4-32, FEP , CTX , CFS 4-32, CAZ , CIP , CO 0.5-2, FF 16-64, GM 1-8, IPM 1-8, LVX , MEM 1-8, MXF 0.5-4, PIP 4-64, TZP 4-64, TIC 8-64, NN 1-8, SXT 1-4 BD Phoenix AST Only Panel (PMIC-55) List of Antimicrobials(μg/ml): AN 8-32, AMC 1-4, AM 2-8, FOX 2-8, CIP , CC 0.5-2, E , FA 1-8, GM 1-8, GMS 500 -, LZD 0.5-4, MUP 1-4, MUH - 256, FM 16-64, OX , P , SYN 0.5-2, RA 0.5-2, TEC 1-16, TE 0.5-8, NN 2-8, TMP 0.5-2, SXT 1-4, VA 1-16 BD Phoenix AST Only Panel (PMIC 58) List of Antimicrobials(μg/ml): AMC 1-4 / 0.5-2, AM 1-8, FOX 2-8, CIP 0.5-2, CC 0.5-2, E , FF , FA 2-8, GM 2-8, GMS 500, LVX 1-4, LZD 1-4, MXF 0.5-2, MUP 1-4, MUH 256, FM 16-64, OX , P , SYN 0.5-2, RA 0.5-2, TEC 1-16, TE 0.5-8, TMP 2-8, SXT / , VA 1-16 BD Phoenix AST Only Panel (PMIC-60) List of Antimicrobials(μg/ml): FOX 2-8, DAP 1-4, E , FF 16-64, FA 2-16GM 0.5-8, K 4-16, LVX 0.5-4, L 1-8, LZD 0.5-4, MOX 2-16, OX , P , PR , RA , TEC 1-32, TE 0.5-8, NN 1-4, SXT 2-8, VA BD Diagnostics Diagnostic Systems Catalog 2008/2009

185 Identification & Susceptibility Testing BD Phoenix Automated Identification & Susceptibility Testing BD Phoenix AST Only Panels x 4.5 ml BD Phoenix AST Only Panel (PMIC-64) List of Antimicrobials(μg/ml): AM 2-8, FOX 2-16, CIP 0.5-2, CC 0.5-4, ETP 0.5-4, E , FF , FA 2-16, GM 1-8, GMS 500, IPM 2-8, LVX 0.5-4, LZD 1-4, MEM 2-8, MXF 0.5-2, MUH 256, OX , P , RA 0.5-2, STS 1000, TEC 1-16, TE 0.5-8, SXT 0.5-2, VA 1-16 BD Phoenix AST Only Panel (PMIC-65) List of Antimicrobials (μg/ml): AM , SAM 4/2-16/8, FOX 2-16, CIP 0.5-2, CC 0.5-2, E , FF , FA 4-16, GM 2-8, GMS 500, IPM 2-8, LVX 1-4, LZD 1-4, MEM 2-8, MXF 0.5-2, OX , P , RA 0.5-2, STS 1000, TEC 1-16, TE 0.5-8, NN 2-8, SXT 0.5/9.5-2/38, VA 1-16 BD Phoenix - ID Broth The BD Phoenix ID broth is a modified saline solution that, after addition of bacteria, is used for identification of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 4.5 ml. 1 tube of ID Broth is sufficient to inoculate 1 BD Phoenix panel x 2.2 ml BD Phoenix - Low Volume Broth The BD Phoenix low volume broth is used in case of sparse colony growth in order to reach either a 0.25 or 0.5 McFarland, respectively. In case of an 0.25 McFarland adjustment, 50 μl of ID broth must be transferred to an AST broth instead of 25 μl (this procedure can only be done by using the BD PhoenixSpec Cat.No ). The low volume broth is a modified saline solution that, after addition of bacteria, is used only for susceptibility testing of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 2.2 ml. 1 tube of low volume broth is sufficient to inoculate 1 BD Phoenix panel x 8 ml BD Phoenix - AST Broth The BD Phoenix AST broth is modified Mueller Hinton Broth that, after the addition of AST indicator and bacterial suspension, is used for susceptibility testing of microorganisms by the BD Phoenix system. The broth is supplied in screw-cap tubes with a volume of 8.0 ml. 1 tube of AST Broth is sufficient to inoculate 1 BD Phoenix panel x 6 ml BD Phoenix - AST Indicator The BD Phoenix AST indicator is a redox indicator that, after addition to AST broth, is used for susceptibility testing of microorganisms by the BD Phoenix system. Each 6 ml bottle of AST Indicator is sufficient for the inoculation of 100 AST Broth tubes, i.e. 1 package (10 bottles) is sufficient for 1000 tubes. BD Diagnostics Diagnostic Systems Catalog 2008/

186 Identification & Susceptibility Testing BD Microtrol Quality Control Organisms BD Microtrol Single Strains discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs BD Microtrol - Aeromonas hydrophila, ATCC 7966 BD Microtrol - Aspergillus niger, ATCC BD Microtrol - Bacillus cereus, ATCC 7464 BD Microtrol - Bacillus subtilis, ATCC 6633 BD Microtrol - Bacteroides fragilis, ATCC BD Microtrol - Campylobacter jejuni, NCTC BD Microtrol - Candida albicans, ATCC BD Microtrol - Candida albicans, ATCC 2091 BD Microtrol - Citrobacter freundii, ATCC 8090 BD Microtrol - Clostridium perfringens, ATCC BD Microtrol - Clostridium sporogenes, ATCC BD Microtrol - Enterobacter aerogenes, ATCC BD Microtrol - Enterobacter cloacae, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus hirae, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC 8739 BD Microtrol - Haemophilus influenza, ATCC BD Microtrol - Klebsiella aerogenes, NCTC 9528 BD Microtrol - Klebsiella pneumonia, ATCC BD Diagnostics Diagnostic Systems Catalog 2008/2009

187 Identification & Susceptibility Testing BD Microtrol Quality Control Organisms BD Microtrol Single Strains discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs discs BD Microtrol - Klebsiella pneumoniae, NCTC BD Microtrol - Listeria monocytogenes, NCTC BD Microtrol - Listeria monocytogenes, NCTC 7973 BD Microtrol - Neisseria Gonorrhoeae, NCTC 8375 BD Microtrol - Proteus mirabilis, ATCC BD Microtrol - Proteus vulgaris, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC 9027 BD Microtrol - Salmonella poona, NCTC 4840 BD Microtrol - Salmonella typhimurium, ATCC BD Microtrol - Serratia marcescens, ATCC 8100 BD Microtrol - Serratia marcescens, ATCC 8100 BD Microtrol - Staphylococcus aureus, ATCC BD Microtrol - Staphylococcus aureus, ATCC BD Microtrol - Staphylococcus aureus, ATCC 6538 BD Microtrol - Staphylococcus aureus, ATCC 6538 P BD Microtrol - Staphylococcus aureus, NCTC 6571 BD Microtrol - Staphylococcus aureus (MRSA), NCTC BD Microtrol - Staphylococcus epidermidis, ATCC BD Microtrol - Streptococcus agalactiae, ATCC BD Microtrol - Streptococcus agalactiae, ATCC BD Microtrol - Salmonella Nottingham, ATCC 7832 BD Diagnostics Diagnostic Systems Catalog 2008/

188 Identification & Susceptibility Testing BD Microtrol Quality Control Organisms BD Microtrol Single Strains discs discs discs discs BD Microtrol - Streptococcus pneumoniae, ATCC 6303 BD Microtrol - Streptococcus pneumoniae, NCTC BD Microtrol - Streptococcus pyogenes, ATCC BD Microtrol - Yersinia enterocolitica, ATCC BD Diagnostics Diagnostic Systems Catalog 2008/2009

189 Blood Culture Systems BD Diagnostics offers a variety of BD BACTEC Blood Culturing instruments and media to meet the wide range of blood culture demands that are put on hospital and reference laboratories. The combination of BD BACTEC instruments and BD BACTEC blood culture media help to improve patient diagnosis and treatment and reduce health care costs by optimizing septicemia diagnosis and maxi mizing laboratory efficiency, safety and produc tivity. The BD BACTEC instruments support the complete line of BD BACTEC /F media for the recovery of bacteria, yeasts and fungi present in blood. The following section describes the BD BACTEC instrument options and media choices available. The choice of instruments and media will provide your facility with the proper capacity of instrumentation and the best possible recovery characteristics for your specific needs. BD BACTEC TM 9000 Series: Instruments 8-2 BD BACTEC TM 9000 Series: Media & Supplement Kit BD Vacutainer TM Safety Solutions for Blood Collection & Subculturing BD BACTEC TM 9000 Series: Accessories 8-5

190 Blood Culture Systems BD BACTEC 9000 Series BD BACTEC Instruments BD BACTEC 9000 blood culture system is designed for the rapid, cost-efficient recovery and detection of bacteria, yeasts, fungi and mycobacteria in clinical cultures of blood BD BACTEC - Blood Culture System BD BACTEC Features include continuous monitoring of culture specimens, noninvasive design, unique fluorescent sensor technology, reliability and robustness, and simple operation. The BD BACTEC 9050 blood culture system has a capacity of 50 BD BACTEC blood culture vials and is best suited for laboratories processing 5 blood culture bottle pairs per day BD BACTEC - Blood Culture System BD BACTEC Features include continuous monitoring of culture specimens, noninvasive design, unique fluorescent sensor technology, reliability and robustness, and simple operation. Data management of patient information is possible through a bi-directional LIS-interface or through the BD EpiCenter Data Management System (see chapter 11 of this catalog) connected to the LIS. The BD BACTEC 9120 blood culture system has a capacity of 120 BD BACTEC blood culture vials and is best suited for laboratories processing blood culture bottle pairs per day BD BACTEC - Blood Culture System BD BACTEC Features include continuous monitoring of culture specimens, noninvasive design, unique fluorescent sensor technology, reliability and robustness, and simple operation. Data management of patient information is possible through a bi-directional LIS-interface or through the BD EpiCenter (see chapter 11 of this catalog) Data Management System connected to the LIS. The BD BACTEC 9240 blood culture system has a capacity of 240 BD BACTEC blood culture vials and is best suited for laboratories processing blood culture bottle pairs per day. 8-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

191 Blood Culture Systems BD BACTEC 9000 Series BD BACTEC Media & BD FOS Supplement Kit vials BD BACTEC PLUS - Aerobic/F Medium BD BACTEC PLUS - Aerobic/F Medium (enriched soybean-casein digest broth with CO2) is used for the culture and recovery of aerobic microorganisms from blood specimens with enhanced recovery from antibiotic patients. Optimal results are obtained when 10 ml of blood is used. The Vials contain resin for neutralization of antimicrobials, thus increasing the recovery of microorganisms from patients under antibiotic treatment vials BD BACTEC PLUS - Anaerobic/F Medium BD BACTEC PLUS - Anaerobic/F Medium Culture Vials (prereduced enriched Soybean-Casein Digest broth with CO2) are used for the culture and recovery of anaerobic microorganisms (bacteria and yeasts) from blood specimen. This medium has been designed to allow the addition of 10 ml of blood. The addition of these larger volumes results in overall higher detection rates and earlier times to detection. The Vials contain resin for neutralization of antimicrobials, thus increasing the recovery of microorganisms from patients under antibiotic treatment vials BD BACTEC - Peds Plus Medium BD BACTEC - Peds Plus Medium Culture Vials (enriched Soybean-Casein Digest broth with CO2) are used for the culture and recovery of aerobic microorganisms (mainly bacteria and fungi) from pediatric and other blood specimen which are generally less than 3 ml in volume. The Vials contain resin for neutralization of antimicrobials, thus increasing the recovery of microorganisms from patients under antibiotic treatment vials BD BACTEC - Lytic/10 Anaerobic/F Medium BD BACTEC - Lytic/10 Anaerobic/F Medium Culture Vials (prereduced enriched Soybean- Casein Digest broth with CO2) are used for the culture and recovery of anaerobic microorganisms from blood specimen. BD BACTEC Lytic/10 Anaerobic/F vials contain saponin in order to release phagocytized microorganisms from leukocytes thus increasing the recovery rate and reducing false-positives. This medium has been designed to allow the addition of 3-10 ml of blood. The addition of larger volumes results in overall higher detection rates and earlier times to detection. This medium does not contain resins intended for the neutralization of antibiotics. The Vials provide faster time to detection for facultative and anaerobic organisms compared to standard and PLUS anaerobic media vials vials BD BACTEC - Mycosis IC/F Medium Culture Vials BD BACTEC - Mycosis IC/F Medium Culture Vials are used for the selective culture and recovery of fungi and yeasts from blood. The medium contains tobramycin and chloramphenicol in order to suppress bacterial growth and saponin in order to release phagocytized fungi and yeasts from leukocytes. Time to detection of fungi and yeasts is greatly reduced compared to nonselective blood culture media. Acceptable blood volumes are ranging from 8-10 ml. The Vials are specially designed for immunosupressed, neutropenic patients vials BD BACTEC - Myco/F Lytic Medium BD BACTEC - Myco/F Lytic (a modified Middlebrook 7H9 broth) when used with the BD BACTEC 9000 Blood Culture series of instruments is a non-selective culture medium to be used as an adjunct to aerobic blood culture media for the recovery of mycobacteria, yeast and fungi. This media may also be used for the culture of sterile body fluids when yeast or fungi are suspected. One advantage of this medium is direct inoculation of blood into the blood culture bottle without the need for supplements. Inoculation of blood volumes ranging between 1-5 ml is acceptable, but optimum recovery is obtained with 3-5 ml. This medium is very useful for the characterization of bloodstream infections of AIDS patients. BD Diagnostics Diagnostic Systems Catalog 2008/

192 Blood Culture Systems BD BACTEC 9000 Series BD BACTEC Media & BD FOS Supplement Kit vials BD BACTEC - Standard Anaerobic/F Medium BD BACTEC - Standard Anaerobic/F Medium (enriched soybean-casein digest broth with CO2) is used for the culture and recovery of anaerobic microorganisms from blood specimens. Optimal results are obtained when 10 ml of blood is used. This medium does not contain resins intended for antibiotic neutralization vials BD BACTEC - Standard/10 Aerobic/F Medium BD BACTEC - Standard/10 Aerobic/F Medium (enriched soybean-casein digest broth with CO2) is used for the culture and recovery of aerobic microorganisms from blood specimens. Optimal results are obtained when 10 ml of blood is used. This medium does not contain resins intended for antibiotic neutralization kit BD BACTEC - BD FOS Culture Supplement Kit BD BACTEC FOS (Fastidious Organism Supplement) Culture Supplement Kit is a fastidious organisms supplement (containing NAD and hemin) for BD BACTEC blood culture media which supports the growth of fastidious and/or SPS sensitive bacteria in the absence of blood. Its use is recommended for sterile body fluids other than blood, particularly for CFS specimen. BD FOS is provided in a lyophilized form along with a special BD BACTEC FOS Reconstituting Fluid (BD FOS RF) for use with BD BACTEC culture media to enhance the growth of fastidious organisms, such as Haemophilus spp. and Neisseria spp. The kit consists of four small amber vials of lyophilized FOS Culture Supplement and one larger clear vial of BD FOS Reconstituting Fluid. 1 set is sufficient to inoculate blood culture vials. BD Vacutainer Safety solutions for blood collection Advantages: minimized risk of needle stick injuries; easy handling sets sets pcs pcs. BD Vacutainer Safety-Lok Blood Collection Set Blood Collection Set with safety re-shielding cover and Luer adapter, 21 G ¾", 18 cm tubing, green. Recommended for adults. One case consists fo 4 x 50 Blood Collection Sets. One Set is necessary for a pair of blood culture bottles. BD Vacutainer Safety-Lok Blood Collection Set (Pediatric Use) BD Vacutainer Safety-Lok Blood Collection Set with safety re-shielding cover and Luer adapter, 23 G ¾", 18 cm tubing, light blue. Recommended for pediatric use. One case consists of 4 x 50 Blood Collection Sets. One blood collection system is necessary for one blood culture bottle. BD Vacutainer One Use Holder The combination of the BD Safety-Lok Blood Collection Set (Cat. Nos and ) and the BD Vacutainer One Use Holder allows the closed and safe sampling of blood for inoculation into BD BACTEC 9000 blood culture bottles. BD Vacutainer holder One use holder out of plastic for tubes of 13 mm and 16 mm diameter; white. 8-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

193 Blood Culture Systems BD BACTEC 9000 Series BD Vacutainer Safety solutions for blood culture subculturing Advantages: minimized risk of needle stick injuries; easy handling pcs pcs pcs pcs. BD Vacutainer Luer Adapter Used together with the BD Vacutainer One Use Holder ( ) for safe and easy subculturing. BD Vacutainer Direct Draw Adapter Ready to Use Safe-Device for subculturing of positive BD BACTEC 9000 vials. BD Vacutainer One Use Holder The combination of the BD Safety-Lok Blood Collection Set (Cat. Nos and ) and the BD Vacutainer One Use Holder allows the closed and safe sampling of blood for inoculation into BD BACTEC 9000 blood culture bottles. BD Vacutainer holder One use holder out of plastic for tubes of 13 mm and 16 mm diameter; white. BD Vacutainer Safety solutions for direct ID/AST from positive blood cultures Advantages: faster time to result, no subculturing, safe & easy handling pcs pcs tubes BD Plastipak 10 ml Luer-Lok Syringe 3-Piece single use syringe. Used for the direct ID/AST of Gram negative bacteria with the BD Phoenix from instrument positive blood culture vials. BD Vacutainer Blood Transfer device Used for the direct ID/AST of Gram negative bacteria with the BD Phoenix from instrument positive blood culture vials. BD Vacutainer SST II Advance Used for the direct ID/AST of Gram negative bacteria with the BD Phoenix from instrument positive blood culture vials. BD Diagnostics Diagnostic Systems Catalog 2008/

194 Blood Culture Systems BD BACTEC 9000 Series BD BACTEC Accessories pcs pcs pcs pcs pcs pcs pcs BD BACTEC - Barcode Labels Extra Vial Sequence barcode labels can be used to replace damaged or unreadable labels on culture vials. The barcode contain sequence numbers which uniquely identify each vial : For BD Peds Plus Medium : Universal labels for BD BACTEC Media : For BD BACTEC Lytic/10 Anaerobe F Medium : For BD BACTEC Mycosis IC/F Medium : For BD BACTEC PLUS Aerobe Medium : For BD BACTEC PLUS Anaerobe Medium. BD BACTEC - Lamps Back up lamps for display on facefront of BD Bactec Instruments 9120 and BD BACTEC - Bottle Holder Plastic vial holder to protect BD BACTEC 9000 bottles during transport in pneumatic tube systems. BD BACTEC - Plug Plug to lock defective/bad station in the BD BACTEC Instrument. BD BACTEC - Transport box for Blood Culture Bottles Transport box out of styrofoam for 4 BD BACTEC blood culture bottles. BD BACTEC - Temperature Test Vials For BD BACTEC 9210 and BD BACTEC - Vial Tray Stand out of stainless steel for BD BACTEC vials; for 12 bottles of BD BACTEC 9000 instrument series. BD BACTEC Stands BD BACTEC - Stands : Single-Stand; for one BD BACTEC 9240 or one BD BACTEC : Combo-Stand; for one BD BACTEC 9240 and one BD BACTEC : Computer-Stand (single); for one BD BACTEC computer and one printer : Computer-Stand (double); for two BD BACTEC BD Diagnostics Diagnostic Systems Catalog 2008/2009

195 Mycobacteria Testing BD Diagnostic Systems offers an extensive line of products for your mycobacteriology laboratory. These include specimen collection kits and specimen digestion/decontamination kits, prepared plates and tubes in a variety of formulations and formats, stains, susceptibility test discs and various brothbased systems. In this section you will find the innovative BD BACTEC MGIT 960 System, now featuring plastic tubes for enhanced safety and antimicrobial susceptibility testing. BD Mycobacteria Specimen Collection, Processing & Staining 9-2 BD Manual Mycobacteria Testing 9-5 BD BACTEC TM MGIT TM Automated Mycobacteria Testing 9-11

196 Mycobacteria Testing Systems BD Mycobacteria Specimen Collection, Processing & Staining BD Falcon Mycobacteria Specimen Collection BD Falcon - Sputum Collection Kit Engineered for safer sputum collection, transport and handling the patented BD Falcon Sputum Collection System is designed to help to protect clinicians, laboratorians and uninfected patients from potential sputum pathogens such as mycobacterium. The BD Falcon Sputum Collection System allows for the specimen to be collected directly into a shatter-resistant conical BD Falcon tube, a protective flap then seals off the collection end. The unique hinged design allows for a screw cap to be tightened over the specimen tube without the clinician touching the cap or the collection end of the tube. Ultimately the sputum specimen is delivered to the lab secure and already in the processing tube eliminating the risk inherent when a generic container is used for collection which requires the lab to pour sputum into a separate processing tube. The Kit contains 72 collection systems, including 50 ml conical BD Falcon Tubes. BD MycoPrep Mycobacteria Specimen Digestion & Decontamination x 75 ml x 150 ml BD MycoPrep - Specimen Digestion/Decontamination Kit This kit contains reagents necessary for the preparation of respiratory and non-respiratory (blood excluded) specimens for identification of mycobacteria. The kit includes bottles of NaOH-citrate solution (containing a plastic ampule with NALC) and powdered phosphate buffer. After activation of the NALC ampule, the NALC-NaOH mixture is stable for 24 hours. This kit should be stored at room temperature contains 5 packages of powdered phosphate buffer (ph 6.8) contains 10 packages of powdered phosphate buffer (ph 6.8). BD Rapid and Cold Acid-Fast Procedure (TB Quick Stain) kit TB Quick Stain - Kit For a more rapid version of staining mycobacteria by the cold acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Quick Stain Carbolfuchsin & TB Quick Stain Methylene Blue x 250 ml x 250 ml TB Quick Stain - Carbolfuchsin Individual component for TB Quick-Stain-Kit ( ) for a more rapid version of staining mycobacteria by the cold acid-fast procedure. TB Quick Stain - Methylene Blue Individual component for TB Quick Stain Kit ( ) fro a more rapid version of staining mycobacteria by the cold acid-fast procedure. 9-2 BD Diagnostics Diagnostic Systems Catalog 2008/2009

197 Mycobacteria Testing Systems BD Mycobacteria Specimen Collection, Processing & Staining BD Ziehl-Neelsen (hot) Acid-Fast Procedure kit TB Stain Kit ZN For staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Carbolfuchsin ZN, TB Decolorizer & TB Methylene Blue x 250 ml x 250 ml x 250 ml TB Carbolfuchsin ZN Individual component of the TB Stain Kit ZN ( ) for staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. TB Decolorizer Individual component of the TB Stain Kit ZN ( ) and TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) and Ziehl-Neelsen (hot) acid-fast procedures. TB Methylene Blue Individual component of the TB Stain Kit ZN ( ) for staining mycobacteria by the Ziehl-Neelsen (hot) acid-fast procedure. May also be used for staining mycobacteria by the Kinyoun (cold) acid-fast procedure. BD Kinyoun (cold) Acid-Fast Procedure kit TB Stain Kit K For staining mycobacteria by the Kinyoun (cold) acid-fast procedure. Each kit contains 1 bottle (250 ml) each of TB Carbolfuchsin KF, TB Decolorizer & TB Brilliant Green K x 250 ml x 250 ml x 250 ml TB Brilliant Green K Individual component of the TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) acid-fast procedures. May be used also for staining mycobacterial by the Ziehl- Neelsen (hot) acid-fast procedures. TB Carbolfuchsin KF Individual component of the TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) acid-fast procedure. TB Decolorizer Individual component of the TB Stain Kit ZN ( ) and TB Stain Kit K ( ) for staining mycobacteria by the Kinyoun (cold) and Ziehl-Neelsen (hot) acid-fast procedures. BD Diagnostics Diagnostic Systems Catalog 2008/

198 Mycobacteria Testing Systems BD Mycobacteria Specimen Collection, Processing & Staining BD TB Fluorescent Stain Kits kit TB Fluorescent Stain Kit M For staining mycobacteria by the Morse, Blair, Weiser and Sproat fluorescent procedure. Each kit contains 1 bottle (250 ml) each of TB Auramine M, TB Decolorizer TM & TB Potassium Permanganate kit TB Fluorescent Stain Kit T For staining mycobacteria by the Truant, Brett and Thomas fluorescent procedure. Each kit contains 1 bottle (250 ml) each of TB Auramine-Rhodamine T, TB Decolorizer TM & TB Potassium Permanganate ml x 250 ml x 250 ml x 250 ml x 250 ml x 250 ml Acridine Orange Stain For detecting microorganisms in direct smears by the fluorescent staining technique. Recommended for use in the fluorescent microscopic detection of microorganisms in direct smears prepared from clinical and non-clinical materials. It is particularly useful in the rapid screening of normally sterile specimens, such as cerebrospinal fluid, where few organisms may be present and in the rapid examination of blood smears or smears containing proteinaceous material where differentiation of organisms from background material may be more difficult. TB Auramine M Individual component of the TB Fluorescent Stain Kit M ( ) for staining mycobacteria by the Morse, Blair, Weiser and Sproat fluorescent procedure. TB Auramine-Rhodamine T Individual component of the TB Fluorescent Stain Kit T ( ) for staining mycobacteria by the Truant, Brett and Thomas fluorescent procedure TB Decolorizer TM Individual component of the TB Fluorescent Stain Kits M and T (Cat. Nos and ) for staining mycobacteria by the Truant, Brett and Thomas and the Morse, Blair, Weiser and Sproat fluorescent procedures. TB Potassium Permanganate Individual component of the TB Fluorescent Stain Kits M and T (Cat. Nos and ) for staining mycobacteria by the Truant, Brett and Thomas and the Morse, Blair, Weiser and Sproat fluorescent procedures. Accessories & Supplies for Acid-Fast Procedures pcs. Acid Fast Bacilli (AFB) Quality Control Slide The Acid fast Bacilli (AFB) QC Slides are conventional 1 x 3 microscope slides imprinted with two circles. One circle is an acid-fast positive control with Staphylococcus aureus and Bacillus subtilis plus inactivated Mycobacterium tuberculosis H37Ra. The other circle is a negative control with an unstained mixture of non-acid-fast cocci (Staphylococcus aureus) and bacilli (Bacillus subtilis). AFB Slides, individually wrapped, sufficient for 50 tests. 9-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

199 Mycobacteria Testing Systems BD Manual Mycobacteria Testing & DCM & Enrichments for Mycobacteria Testing Loewenstein Medium Base Media for the growth and recovery of Mycobacteria. Can be used with glycerol to prepare a variety of coagulated egg media. Middlebrook 7H9 Broth For cultivation of mycobacteria and preparation of tubercle emulsion for susceptibility testing. Middlebrook ADC Enrichment (Cat. Nos or ) and Glycerol or Tween 80 must be added to the Broth before use Middlebrook 7H10 Agar Used for the isolation, cultivation and susceptibility testing of mycobacteria. Middlebrook OADC Enrichment (Cat. Nos or or ) and Glycerol must be added; the complete Prepared Plated Medium (with OADC) is available under , Middlebrook and Cohn 7H10 Agar (see chapter 4-2 of this catalogue) Middlebrook 7H11 Agar Base Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Middlebrook OADC Enrichment (Cat. Nos or or ) and Glycerol must be added to the Agar Base before use x 100 ml x 20 ml Middlebrook ADC Enrichment Used to supplement culture media for the cultivation of Mycobacteria (for Middlebrook 7H9 Broth, ) : Prepared Tubed Media: 10 x 20 ml per tube, "A" size tube : Prepared Bottled Media: 6 x 100 ml per bottle ml x 20 ml x 100 ml Middlebrook OADC Enrichment Used to supplement culture media for the isolation and cultivation of Mycobacteria for Middlebrook 7H10 Agar, Cat. Nos and and Mycobacteria 7H11 Agar, : Prepared Tubed Media: 10 x 20 ml per Tube, "A" size Tube : Prepared Bottled Media: 6 x 100 ml per Bottle : Prepared Bottled Media: 1 x 500 ml per Bottle. BD Diagnostics Diagnostic Systems Catalog 2008/

200 Mycobacteria Testing Systems BD Manual Mycobacteria Testing Prepared Media (PPMs & Tubes) for Mycobacteria Testing PPMs on BD Stacker Format Plates and Prepared Tubed Media (A = 20 mm x 148 mm, C = 20 x 112 mm, K = 16.5 mm x 102 mm) tubes tubes tubes tubes tubes deeps slants slants slants slants bottles slants tubes tubes Gottsacker TB Medium Solid egg-based media for the recovery and culture of Mycobacteria. Prepared Tubes, Size A. Herrold's Egg Yolk Agar with Mycobactin J and ANV Used for the selective isolation and differentiation of Mycobacterium paratuberculosis. ANV (amphotericin, nalidixic acid and vancomycin) inhibits contamination and Mycobactin J is a growth factor for M. paratuberculosis. Prepared Tubes, Size C. Herrold's Egg Yolk Agar without Mycobactin J with ANV Used for the selective isolation and differentiation of mycobacteria other than Mycobacterium paratuberculosis. ANV (amphotericin, nalidixic acid and vancomycin) inhibits contamination. M. paratuberculosis is unable to grow on media lacking Mycobactin J. (For use with Herrold's Egg Yolk Agar with Mycobactin J and ANV, Cat. Nos or ). Prepared Tubes, Size C. Loewenstein-Jensen Medium For Semi-Qualitative Catalase Test. Loewenstein-Jensen Medium Deeps contain an enriched eggbased medium and are used for the cultivation of mycobacteria, including Mycobacterium tuberculosis. Deeps are tubes of hardened agar medium which are inoculated by "stabbing" the inoculum into the agar. Used for for Semi-Qualitative Catalase Test : Prepared Deeps, Tubes Size A & : Prepared Slants, Tubes Size A & : Prepared Slants, Tubes Size C Loewenstein-Jensen Medium (BD Mycoflask ) An enriched egg-based medium used for the cultivation of Mycobacterium tuberculosis (causative agent of tuberculosis) and other mycobacterial species. The long shelf life of this product is ideal for laboratories that process only a few specimens. This medium comes in a BD Mycoflask Bottle. Loewenstein-Jensen Medium with 5 % Sodium Chloride L-J Medium Slants with 5 % NaCl are intended for the isolation and cultivation of mycobacteria that can tolerate 5 % Sodium chloride (e.g. M. fortuitum and M. chelonae subsp. absessus). Prepared Slants, Tubes Size C. Loewenstein-Jensen Medium with Glycerol and PACT Loewenstein-Jensen Medium Slants with Glycerol and PACT are used for the cultivation of mycobacteria, especially M. tuberculosis. It is a selective medium containing polymyxin, amphotericin, carbenicillin, and trimethoprim (PACT). Prepared Tubes, Size A. Middlebrook 7H10 // 7H11 Selective Agar Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. These bi-plates provide two media, one moderately selective and one highly selective, for the recovery of mycobacteria from specimens containing mixed flora. The Middlebrook 7H11 Agar is contained in the sector differentiated by the Roman numeral "I" molded into the bottom of the sector. The 7H11 Selective Agar is contained in the sector marked "II." x 5 ml Middlebrook 7H9 Broth with Glycerol Middlebrook 7H9 Broth with Glycerol is a nonselective liquid culture medium for the cultivation of mycobacteria, including M. tuberculosis. It is used primarily for growth of pure cultures of mycobacteria for use in laboratory studies. Prepared Tubes, 5 ml, Size K. 9-6 BD Diagnostics Diagnostic Systems Catalog 2008/2009

201 Mycobacteria Testing Systems BD Manual Mycobacteria Testing Prepared Media (PPMs & Tubes) for Mycobacteria Testing PPMs on BD Stacker Format Plates and Prepared Tubed Media (A = 20 mm x 148 mm, C = 20 x 112 mm, K = 16.5 mm x 102 mm) slants slants slants slants slants tubes tubes Middlebrook and Cohn 7H10 Agar Middlebrook 7H10 Agar contains a variety of inorganic salts which provide substances essential for the growth of mycobacteria. The sodium citrate, when converted to citric acid, holds certain inorganic cations in solution. Glycerol is an abundant source of carbon and energy. Oleic acid, as well as other long chain fatty acids, can be utilized by tubercle bacilli and plays an important role in the metabolism of mycobacteria. Catalase destroys toxic peroxides that may be present in the medium. The primary effect of albumin is that of protection of the tubercle bacilli against toxic agents, therefore enhancing their recovery by primary isolation. Partial inhibition of bacteria is achieved by the presence of the malachite green dye. Middlebrook and Cohn 7H10 Agar Middlebrook and Cohn 7H10 Agar is a culture medium for the cultivation of mycobacteria, especially mycobacteria other than tuberculosis (MOTT) : Prepared Slants, Tubes Size A : Prepared Slants, Tubes Size A : Prepared Slants, Tubes Size C Seven H11 Agar (Deep Fill) Used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Prepared Plates, Deep Fill. Seven H11 Agar (Middlebrook 7H11 Agar) Seven H11 Agar (also referred to as Middlebrook 7H11 Agar). The media is used in qualitative procedures for isolation and cultivation of mycobacteria, especially Mycobacterium tuberculosis, from clinical and nonclinical specimens. Prepared Slants, Tubes Size A. Seven H11 Agar with Aspartic Acid and Sodium Pyruvate Used for isolation and identification of nonchromogenic mycobacteria, especially M. tuberculosis. The aspartic acid is included to increase the rate of niacin production. Prepared Slants, Tubes Size A. Stonebrink TB Medium with PACT Stonebrink TB Medium with PACT Selective Medium is intended for the isolation of mycobacteria, especially Mycobacterium tuberculosis. It is a selective medium containing Polymyxin, Amphotericin, Carbenicillin, and Trimethoprim (PACT). Prepared Tubes, Size A. BD Diagnostics Diagnostic Systems Catalog 2008/

202 Mycobacteria Testing Systems BD Manual Mycobacteria Testing BD Sensi-Disc for Mycobacteria Susceptibility Testing discs discs discs discs discs discs x 50 discs x 50 discs discs BD Sensi-Disc - Ethambutol, 25 μg (EM-25) BD Sensi-Disc - Ethambutol, 50 μg (EM-50) BD Sensi-Disc - Ethionamide, 25 μg (EA-25) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 1 μg (INH-1) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 5 μg (INH-5) BD Sensi-Disc - Rifampin, 25 μg (RA-25) BD Sensi-Disc - Rifampin, 5 μg (RA-5) BD Sensi-Disc - Streptomycin, 10 μg (S-10) BD Sensi-Disc - Streptomycin, 50 μg (S-50) 9-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

203 Mycobacteria Testing Systems BD Manual Mycobacteria Testing BD Taxo Identification & Differentiation of Mycobacteria strips BD Taxo - INH Test Strips BD Taxo INH Test Strips are used for the detection of isoniazid (INH) and its metabolites in urine. When INH or its metabolites are present in a urine sample, contact with the BD Taxo INH strip will change the strip color to green, blue or purple. This strip is a modification of Kilburn and Kubica s procedure for detecting niacin produced by M. tuberculosis. White paper strips encased in a plastic tube. Arrows are imprinted on both sides. Sufficient for 25 tests; storage should be at 2-8 C discs strips BD Taxo - INH Test Control The BD Taxo INH Test Control is an impregnated disc that will yield a positive result in the test procedure. For use with White, ¼" paper disks with "I"over "C", sufficient for 50 tests. BD Taxo - Nitrite Test Strips BD Taxo Nitrite Test Strips are used for differentiation of microorganisms (particularly mycobacteria) based on the reduction of nitrates to nitrites. The nitrate reduction test separates slow growing mycobacteria that are nitrate-positive (e.g., M. tuberculosis, M. kansasii and M. fortuitum) from rapid growers that are negative or only weakly positive (e.g., M. bovis, M. avium complex and M. intracellulare). The presence of the enzyme nitrate reductase is detected by appearance of a blue-colored end product on the strip, indicating a positive reaction. Sufficient for 25 tests strips discs BD Taxo - TB Niacin Test Strips BD Taxo TB Niacin Test Strips assist in the detection of mycobacteria by detecting niacin in culture medium. All mycobacteria produce nicotinic acid, or niacin, which accumulates in culture medium and can be detected using these strips. When BD Taxo TB Niacin Test Strips turn yellow, the culture is mycobacteria positive and when no color appears, the culture is negative. Contains 1 vial with 25 strips, sufficient for 25 tests. BD Taxo - TB Niacin Test Control BD Taxo TB Niacin Test Controls are impregnated with nicotinamide and give a positive reaction when tested with a TB Niacin Test Strip. For use with BD Taxo TB Niacin Test Trips ( ). Contains 50 discs per hand-dispensing cartridge, sufficient for 50 tests. BD Diagnostics Diagnostic Systems Catalog 2008/

204 Mycobacteria Testing Systems BD Manual Mycobacteria Testing BD MGIT Mycobacteria Growth Indicator Tube s tubes tubes BD MGIT - Tubes (manual, 4 ml) The MGIT Mycobacteria Growth Indicator Tubes contain 4 ml of Middlebrook 7H9 Broth base with a fluorescent indicator and is intended for the manual detection and recovery of mycobacteria. The broth is contained in a plastic tube with screw-top cap and supplemented with BD MGIT OADC enrichment ( ) and BD MGIT PANTA antibiotic mixture ( ). Acceptable specimen types are digested and decontaminated clinical specimens (except urine) and sterile body fluids (except blood). A culture-positive sample is identified by the observation of non-homogenous turbidity or fluorescence, the latter of which is observed using a 365 mm UV transilluminator, a longwave UV light (Wood s lamp) or the BD MicroMGIT Reader vials BD MGIT - OADC Enrichment The BD MGIT OADC enrichment provides substances essential for the rapid growth of mycobacteria in BD MGIT 4 ml Tubes. OADC stands for oleic acid, albumin, dextrose and catalase. 6 ready to use vials containing 15 ml each, sufficient for 25 BD MGIT tubes vials BD MGIT - PANTA Antibiotic Mixture The BD MGIT PANTA Antibiotic Mixture suppresses growth of contaminating or normal flora, enhancing growth and detection of mycobacteria in BD MGIT Tubes. Each vial contains a lyophilized antibiotic mixture of polymyxin B, amphotericin B, nalidixic acid, trimethoprim and azlocillin. Carton of 6 vials, each vial sufficient for 25 BD MGIT tubes. BD MGIT Antimicrobial Susceptibility Testing kit BD MGIT - AST SIRE Test Kit (manual) The BD MGIT AST SIRE Testing Kit is used for susceptibility testing of Mycobacterium tuberculosis in culture to streptomycin, isoniazid, rifampin and ethambutol (SIRE) in a qualitative test lasting 4-13 days. The kit contains 2 vials of each lyophilized antimicrobic (S, I, R, and E) and must be reconstituted with 4 ml of sterile/deionized water. Carton of 8 lyophilized vials, sufficient for 80 tests. BD MGIT System Accessories & Supplies BD BACTEC - MicroMGIT Fluorescence Reader The BD BACTEC MicroMGIT Fluorescence Reader is used for the qualitative reading of fluorescence in BD MGIT Tubes (4 ml). The Reader has a standard 9 volt battery, has a low voltage indicator, and has dimensions of 9.2 cm x 14.5 cm x 12.0 cm. (WxDxH). A calibration tube is included. BD BACTEC - MicroMGIT Calibrator Used for calibration of the BD BACTEC - MicroMGIT Fluorescence Reader ( ) BD Diagnostics Diagnostic Systems Catalog 2008/2009

205 Mycobacteria Testing Systems BD BACTEC MGIT Automated Mycobacteria Testing BD BACTEC MGIT 960 Mycobacteria Testing System BD BACTEC MGIT Mycobacterial Detection Instrument The BD BACTEC MGIT 960 Instrument is a nonradiometric and fully automated system for the rapid detection of mycobacteria in clinical specimens other than blood. The BD BACTEC MGIT 960 is also used for the antimicrobial susceptibility testing of mycobacteria, including SIRE and PZA susceptibility testing. The instrument has a maximum capacity of 960 BBL MGIT tubes (7 ml), or with a 42-day detection protocol, approximately 8000 specimens per year. The BD BACTEC MGIT 960 System, in conjunction with the BD EpiCenter Data Management System, allows for long-term data storage and historical trending as well as a bidirectional interface with a LIS. This enables long-term tracking of individual TB patients, infection control and epidemiology analysis vials vials BD BACTEC MGIT Calibrators Kit 16 vials are sufficient for 1 drawer. BD BACTEC MGIT Positive Tubes Tubes to simultate positivity. BD BACTEC MGIT Temperature Control Tube 3 tubes are needed in each instrument. BD BACTEC MGIT 960 Media for Growth & Detection tubes BD BACTEC MGIT Tubes 7 ml The BACTEC MGIT Tube is intended for the detection and recovery of mycobacteria using the BD BACTEC MGIT 960 system. Each plastic tube has a screw-top cap and contains 7 ml of modified Middlebrook 7H9 Broth base, which is supplemented with BD BACTEC MGIT Growth Supplement ( ). Acceptable specimen types are digested and decontaminated clinical specimens (except urine) and sterile body fluids (except blood). Additionally, these tubes can also be used for antimicrobial susceptibility testing of mycobacteria, including SIRE and PZA testing. MGIT tubes should be stored between 2-25 C kit BD BACTEC MGIT Supplement Kit The kit includes 6 vials of lyophilized BBL MGIT PANTA Antibiotic Mixture and 6 vials of 15 ml BD BACTEC MGIT 960 Growth Supplement. The BD BACTEC MGIT Growth Supplement contains Middlebrook OADC enrichment (Oleic acid, Bovine albumin, Dextrose, Catalase and Polyoxyethylene stearate [POES]). The BBL MGIT PANTA Antibiotic Mixture contains Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim and Azlocillin. The Supplement Kit should be stored between 2-8 C and is sufficient for approximately 100 tests. BD Diagnostics Diagnostic Systems Catalog 2008/

206 Mycobacteria Testing Systems BD BACTEC MGIT Automated Mycobacteria Testing BD BACTEC MGIT 960 Media & Kits for Susceptibility Testing kit BD BACTEC MGIT SIRE Kit The BD BACTEC MGIT 960 SIRE Kit is a rapid qualitative procedure for susceptibility testing of Mycobacterium tuberculosis, from culture, to streptomycin (STR), isoniazid (INH), rifampin (RIF) and ethambutol (EMB). Carton of 4 lyophilized drug vials, and 8 SIRE Supplements. Sufficient for 40 tests kit BD BACTEC MGIT IR Kit The BD BACTEC MGIT 960 IR Kit is used for susceptibility testing of Mycobacterium tuberculosis in culture to the critical concentrations of isoniazid and rifampin (IR) in a qualitative test lasting 4-13 days. The IR Kit contains 1 vial of each lyophilized antimicrobic (I, R) and 4 vials of SIRE Supplement. The antimicrobics must be reconstituted with 4 ml of sterile/deionized water before addition to BBL MGIT tubes. The kit must be stored at 2-8 C and is sufficient for 40 tests kit BD BACTEC MGIT PZA Kit The BD BACTEC MGIT 960 PZA Kit is used for susceptibility testing of Mycobacterium tuberculosis in culture to pyrazinamide (PZA) in a qualitative test lasting 4-17 days. The Kit contains 2 vials of lyophilized antimicrobic and 6 vials of SIRE Supplement.. PZA must be reconstituted with 2.5 ml of sterile/deionized water before addition to BBL MGIT tubes. Carton of 2 lyophilized vials and 6 PZA Supplements, sufficient for 50 tests tubes kit BD BACTEC MGIT PZA Medium The BD BACTEC MGIT 960 PZA Medium is a tube containing modified Middlebrook 7H9 Broth with a reduced ph of 5.9 and is to be used for susceptibility testing of Mycobacteria tuberculosis. BD BACTEC MGIT STR 4.0 Kit The STR (Streptomycin) 4.0 Test Kit is intended for the susceptibility testing of M. tuberculosis to MOP (Method of Proportion) high concentration of streptomycin (4.0 μg/ml). Carton of 1 lyophilized drug vial and 2 SIRE Supplements. Sufficient for 20 tests kit BD BACTEC MGIT INH 0.4 Kit The INH (Isoniazid) 0.4 Test Kit is intended for the susceptibility testing of M. tuberculosis to MOP (Method of Proportion) high concentration of isoniazid (0.4 μg/ml). Carton of 1 lyophilized drug vial and 2 SIRE Supplements. Sufficient for 20 tests kit BD BACTEC MGIT EMB 7.5 Kit The EMB (Ethambutol) 7.5 Test Kit is intended for the susceptibility testing of M. tuberculosis to MOP (Method of Proportion) high concentration of ethambutol (7.5 μg/ml). Carton of 1 lyophilized drug vial and 2 SIRE Supplements. Sufficient for 20 tests BD Diagnostics Diagnostic Systems Catalog 2008/2009

207 Mycobacteria Testing Systems BD BACTEC MGIT Automated Mycobacteria Testing BD BACTEC MGIT 960 Accessories & Supplies kit set set set set set plugs BD BACTEC MGIT AST Starter Kit Includes: User Manual, Quick reference guide, 2 x Transport rack, 16 x 5-tube AST Set carrier, 16 x 2-tube AST Set carrier, 3 x 3-tube AST Set carrier, 3 x 4-tube AST Set carrier and 3 x 8-tube AST Set carrier. BD BACTEC MGIT AST Carrier Set (Spare Barcodes) 5 spare barcodes for each of the AST carrier sets (2-, 3-, 4-, 5-, and 8-tube carrier sets). BD BACTEC MGIT AST Carrier Sets (2-, 3-, 4-, 5-, and 8-tube) One set includes each 3 carriers : AST Carrier Set (2-tube) : AST Carrier Set (3-tube) : AST Carrier Set (4-tube) : AST Carrier Set (5-tube) : AST Carrier Set (8-tube) BD BACTEC MGIT AST Transport Rack BD BACTEC MGIT ml tube Spare Barcode Labels BD BACTEC MGIT Plug, Bad Station 10 Plugs for temporary blocking of an individual station. BD Diagnostics Diagnostic Systems Catalog 2008/

208

209 Molecular Diagnostic The BD ProbeTec ET Systems offer DNA amplification assays for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) for use in public health, physician office, reference and hospital clinical laboratories. These systems are state-of-the-art molecular technologies that are easy to use, have minimal hands-on time, and provide rapid results increasing laboratory efficiency at the most labor-intensive steps for molecular methods, sample handling, amplification and detection. The BD Affirm VPIII tests for the detection of Candida sp., Gardnerella vaginalis, and Trichomonas vaginalis translate the power and precision of RNA probe technology to aid better laboratory practice and patient care in an easy to use format. BD ProbeTec TM ET System 10-2 BD ProbeTec TM ET Consumables 10-4 BD Affirm TM VPIII Microbial Identification Systems BD Diagnostics GeneOhm TM 10-12

210 Molecular Diagnostics BD ProbeTec ET System BD ProbeTec ET Instrument & Equipment for CT, CT/GC, TB and AP Assays BD ProbeTec ET - Instrument Available since 1999, the BD ProbeTec ET System is the first real-time DNA amplification assay for the detection of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (GC), Mycobacteria (TB), and Atypical Pneumonia (AP). Based on the proprietary BD technology, Strand Displacement Amplification (SDA) and real-time homogeneous detection with Energy Transfer (ET), the system design combines short time to results, minimal labor, high throughput and ease-of-use in a compact, one-room setup. The BD ProbeTec instrument performs simultaneous amplification and detection in a real time, one-hour assay format that enhances efficiency by enabling multiple instrument runs per shift. SDA takes place in sealed amplification microwells to prevent amplicon contamination. The system is capable of performing up to 96 reactions simultaneously (including positive and negative controls). Results are reported as positive, negative, or indeterminate. Daily maintenance is less than five minutes for the complete system. LIS connectivity and barcode accession support save time and eliminate errors BD ProbeTec ET - Priming and Warming Heater (220V) The BD ProbeTec ET Priming and Warming Heater is a double warming block with separately controlled temperature settings for the Priming Wells and the Amplification Wells. BD ProbeTec ET - Priming and Warming Heater Thermometer Contains one thermometer for the priming heater and one for the warming heater. BD ProbeTec ET - Pipettor Programmable and automated 8-channel pipettor for the transfer of samples among sample tubes, priming wells, and amplification wells during sample processing. BD ProbeTec ET - Pipettor Stand Metal holder for the pipettor. BD ProbeTec ET - Pipettor Power Supply (220V, 50 Hz) Power supply (200 V, 50 Hz) for the BD ProbeTec ET Pipettor. BD ProbeTec ET - Pipettor Rechargable Batteries Rechargeable batteries for the BD ProbeTec ET Pipettor. BD ProbeTec ET - Pipettor Keypad Cover Protective cover for the BD ProbeTec ET Pipettor keypad. BD ProbeTec ET - Special Oven (all voltage) BD ProbeTec ET special oven (fits all voltage) for sample pretreatment of TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Sample Tube Rack BD ProbeTec ET tube rack out of metall for 2 ml tubes for TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Ultrasonic bath voltage, 50 Hz. Ultrasonic bath for sample pretreatment for TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Pipetting Rack BD ProbeTec ET Pipetting Rack (clear); special rack for sample tubes for for TB diagnostic with the BD ProbeTec ET System BD Diagnostics Diagnostic Systems Catalog 2008/2009

211 Molecular Diagnostics BD ProbeTec ET System BD ProbeTec ET Instrument & Equipment for CT, CT/GC and AP Assays BD ProbeTec ET - Lysing Rack for 96 sample tubes Heat-safe metal rack for securely holding the sample tubes during sample preparation and processing, including lysing samples on the Lysing Heater and allowing tubes to cool. BD ProbeTec ET - Cover for Lysis Block Protective cover for the lysis block in order to avoid accidental contact with the heated lysis block. BD ProbeTec ET - Lysing Heater (220V) Warming block for lysing of probes during sample processing. BD ProbeTec ET - Lysing Thermometer Thermometer for the Lysing Block. BD ProbeTec ET Instrument & Equipment for TB Assays only BD ProbeTec ET - Oven Filter The BD ProbeTec ET Oven Filter filters air that comes out of the Oven. BD ProbeTec ET - Special Oven (all voltage) BD ProbeTec ET special oven (fits all voltage) for sample pretreatment of TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Oven Thermometer The BD ProbeTec ET Oven Thermometer is used to measure the temperature in the BD ProbeTec ET Oven. BD ProbeTec ET - Sample Tube Rack BD ProbeTec ET tube rack out of metall for 2 ml tubes for TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Ultrasonic bath voltage, 50 Hz. Ultrasonic bath for sample pretreatment for TB diagnostic with the BD ProbeTec ET System. BD ProbeTec ET - Pipetting Rack BD ProbeTec ET Pipetting Rack (clear); special rack for sample tubes for for TB diagnostic with the BD ProbeTec ET System. BD Diagnostics Diagnostic Systems Catalog 2008/

212 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for CT Assays tests BD ProbeTec ET - CT reagent pack with AC The BD ProbeTec ET Chlamydia trachomatis (CT) Amplified DNA Assay with Amplification Control (AC), when tested with the BD ProbeTec ET Sysem, uses Strand Displacement Amplification (SDA) technology for the direct and qualitative detection of Chlamydia trachomatis DNA in endocervial swabs, male urethral swabs, and female and male urine specimens as evidence of infection with C. trachomatis. Specimens may be from symptomatic or asymptomatic female and male patients. Contains material for 384 tests tests BD ProbeTec ET - CT reagent pack without AC The BD ProbeTec ET Chlamydia trachomatis (CT) Amplified DNA Assay without Amplification Control (AC), when tested with the BD ProbeTec ET Sysem, uses Strand Displacement Amplification (SDA) technology for the direct and qualitative detection of Chlamydia trachomatis DNA in endocervial swabs, male urethral swabs, and female and male urine specimens as evidence of infection with C. trachomatis. Specimens may be from symptomatic or asymptomatic female and male patients. A separate Amplification Control is an option for inhibition testing (BD ProbeTec ET CT with AC). Contains material for 384 tests tests BD ProbeTec ET - CT/GC Control Set Chlamydia trachomatis (CT) / Neisseria gonorrhoeae (GC) Control Set. Contains a positive and negative control to be included in each test run. Each kit contains reagents for 20 test runs, 20 positive and 20 negative pcs. BD ProbeTec Culturette Direct - Collection and Transport System Used for the sample collection and transport of endocervical specimens. Each BD Culturette Direct Cleaning-Collection and Transport System contains cleaning swabs, collection swabs, and collection tubes (100 of each). Endocervical Specimen Collection and Dry Transport System Pink Cap (female) pcs. BD ProbeTec Mini-Tip BD Culturette Direct Swabs used for the sample collection and transport of urethral specimens from male patients. Urethral Specimen Collection and Dry Transport System Blue Cap (male) pcs. BD ProbeTec ET - Collection Kit for Endocervical Specimens "wet swab" The BD ProbeTec ET Chlamydia trachomatis/neisseria gonorrhoeae (CT/GC) Amplified DNA Assay Collection Kit for Endocervical Specimens is used to collect and transport patient endocervical specimens to the laboratory for testing with the BD ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays. The kit consists of a sterile cleaning swab, a sterile collection swab and a CT/GC Diluent tube for specimen transport. The large rayon-tipped cleaning swab is provided to clean the cervical os of blood and mucus prior to collection. The smaller polyurethane-tipped swab on a plastic shaft is used to collect the specimen for testing. After the specimen is collected, the swab is placed into the CT/GC Diluent tube and the shaft is broken at the score mark. The tube is recapped and transported to the testing facility BD Diagnostics Diagnostic Systems Catalog 2008/2009

213 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for CT Assays pcs. BD ProbeTec ET - Collection Kit for Male Urethral Specimens "wet swab" The BD ProbeTec ET Chlamydia trachomatis/neisseria gonorrhoeae (CT/GC) Amplified DANN Assay Collection Kit for Male Urethral Specimens is used to collect and transport male patient urethral specimens to the laboratory for testing with the BD ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DANN Assays. The Kit consists of a sterile swab and a CT/GC Diluent tube for specimen transport. The rayontipped collection swab consists of a flexible aluminium wire in a plastic shaft. After the specimen is collected, the swab is placed into the CT/GC Diluent tube an the shaft is broken at the score mark. The tube is recapped and tansported to the testing facility pcs pcs x 2 ml bottles tips kits BD ProbeTec ET - Sample Tubes and Caps (4 ml) 4 ml Sample Tubes and Caps that are used for the preparation of urine specimens for an BD ProbeTec ET Assay. BD ProbeTec ET - Sample Tube Caps (4 ml) Replacement caps for the BD ProbeTec ET Sample Tubes. BD ProbeTec ET - Swab Sample Diluent (CT/GC) Transport tubes for collected specimens, either by the Mini-tip BD Culturette Direct or the BD Culturette Direct Cleaning-Collection and Transport System, that are prefilled with 2 ml Sample Diluent. These tubes are intended for use with the CT or CT/GC Assays. BD ProbeTec ET - Diluent (CT/GC) Used for resuspension of the pelleted specimen before lysis and for resuspension of positive and negative controls. Contains 4 bottles of each 225 ml. BD ProbeTec ET - Pipette Tips Sterile aerosol resistant pipette tips for the BD ProbeTec ET Pipettor. Total of 720 tips (6 racks of 120). BD ProbeTec - Urine Preservatie Transport Kit The BD ProbeTec Urine Preservative Transport Kit (UPT), the next generation urine specimen processing system that improves laboratory workflow and sample transport flexibility when testing urine specimens on the BD ProbeTec ET System, is now available. The kit with NAP Guard technology allows for an extended range of time and temperature conditions for specimen storage and transport when testing for the presence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) in urine specimens using the BD ProbeTec ET CT and GC Amplified DNA Assays. In addition, the BD ProbeTec UPT reduces the laboratory processing steps associated with repetitive motion injuries in lab technicians while speeding up the handling of urine samples. Benefits of the BD ProbeTec UPT Kit include: 30-day transport at ambient temperatures (2-30 C) Temperature tolerant during transport (-20 to 60 C) 2-month storage stability at -20 C > 40 % labor reduction compared to the Urine Processing Pouch (UPP) protocol Elimination of 2-hour UPP incubation Low inhibition rates (< 1 %) kit BD ProbeTec ET - Accessories Kit The CT/GC Accessories Kit contains priming plate covers, amplification plate sealers and disposal bags (20 of each per kit). BD Diagnostics Diagnostic Systems Catalog 2008/

214 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for CT/GC Assays tests BD ProbeTec ET - CT/GC Reagent Pack with AC The BD ProbeTec ET Chlamydia trachomatis (CT) / Neisseria gonorrhoeae (GC) Amplified DNA Assays with Amplification Control (AC), when tested with the BD ProbeTec ET system, use Strand Displacement Amplification (SDA) technology for the direct, qualitative detection of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in endocervial swabs, male urethral swabs, and female and male urine specimens as evidence of infection with C. trachomatis, Neisseria gonorrhoeae, or co-infection with both pathogens. Specimens may be from symptomatic and asymptomatic females for the CT assay and GC assay, from symptomatic or asymptomatic males for the CT assay, and from symptomatic males for the GC assays. Contains material for 384 tests tests BD ProbeTec ET - CT/GC Reagent Pack without AC The BD ProbeTec ET Chlamydia trachomatis (CT)/ Neisseria gonorrhoeae (GC) Amplified DNA Assays without Amplification Control(AC), when tested with the BD ProbeTec ET system, use Strand Displacement Amplification (SDA) technology for the direct, qualitative detection of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in endocervial swabs, male urethral swabs, and female and male urine specimens as evidence of infection with C. trachomatis, Neisseria gonorrhoeae, or co-infection with both pathogens. Specimens may be from symptomatic and asymptomatic females for the CT assay and GC assay, from symptomatic or asymptomatic males for the CT assay, and from symptomatic males for the GC assays. A separate Amplification Control is an option for inhibition testing (BD ProbeTec ET CT/GC with AC). Contains material for 384 tests tests BD ProbeTec ET - CT/GC Control Set Chlamydia trachomatis (CT) / Neisseria gonorrhoeae (GC) Control Set. Contains a positive and negative control to be included in each test run. Each kit contains reagents for 20 test runs, 20 positive and 20 negative pcs. BD ProbeTec Culturette Direct - Collection and Transport System Used for the sample collection and transport of endocervical specimens. Each BD Culturette Direct Cleaning-Collection and Transport System contains cleaning swabs, collection swabs, and collection tubes (100 of each). Endocervical Specimen Collection and Dry Transport System Pink Cap (female) pcs. BD ProbeTec Mini-Tip BD Culturette Direct Swabs used for the sample collection and transport of urethral specimens from male patients. Urethral Specimen Collection and Dry Transport System Blue Cap (male) pcs. BD ProbeTec ET - Collection Kit for Endocervical Specimens "wet swab" The BD ProbeTec ET Chlamydia trachomatis/neisseria gonorrhoeae (CT/GC) Amplified DNA Assay Collection Kit for Endocervical Specimens is used to collect and transport patient endocervical specimens to the laboratory for testing with the BD ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assays. The kit consists of a sterile cleaning swab, a sterile collection swab and a CT/GC Diluent tube for specimen transport. The large rayon-tipped cleaning swab is provided to clean the cervical os of blood and mucus prior to collection. The smaller polyurethane-tipped swab on a plastic shaft is used to collect the specimen for testing. After the specimen is collected, the swab is placed into the CT/GC Diluent tube and the shaft is broken at the score mark. The tube is recapped and transported to the testing facility BD Diagnostics Diagnostic Systems Catalog 2008/2009

215 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for CT/GC Assays pcs. BD ProbeTec ET - Collection Kit for Male Urethral Specimens "wet swab" The BD ProbeTec ET Chlamydia trachomatis/neisseria gonorrhoeae (CT/GC) Amplified DANN Assay Collection Kit for Male Urethral Specimens is used to collect and transport male patient urethral specimens to the laboratory for testing with the BD ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DANN Assays. The Kit consists of a sterile swab and a CT/GC Diluent tube for specimen transport. The rayontipped collection swab consists of a flexible aluminium wire in a plastic shaft. After the specimen is collected, the swab is placed into the CT/GC Diluent tube an the shaft is broken at the score mark. The tube is recapped and tansported to the testing facility pcs pcs x 2 ml bottles tips kits BD ProbeTec ET - Sample Tubes and Caps (4 ml) 4 ml Sample Tubes and Caps that are used for the preparation of urine specimens for an BD ProbeTec ET Assay. BD ProbeTec ET - Sample Tube Caps (4 ml) Replacement caps for the BD ProbeTec ET Sample Tubes. BD ProbeTec ET - Swab Sample Diluent (CT/GC) Transport tubes for collected specimens, either by the Mini-tip BD Culturette Direct or the BD Culturette Direct Cleaning-Collection and Transport System, that are prefilled with 2 ml Sample Diluent. These tubes are intended for use with the CT or CT/GC Assays. BD ProbeTec ET - Diluent (CT/GC) Used for resuspension of the pelleted specimen before lysis and for resuspension of positive and negative controls. Contains 4 bottles of each 225 ml. BD ProbeTec ET - Pipette Tips Sterile aerosol resistant pipette tips for the BD ProbeTec ET Pipettor. Total of 720 tips (6 racks of 120). BD ProbeTec - Urine Preservatie Transport Kit The BD ProbeTec Urine Preservative Transport Kit (UPT), the next generation urine specimen processing system that improves laboratory workflow and sample transport flexibility when testing urine specimens on the BD ProbeTec ET System, is now available. The kit with NAP Guard technology allows for an extended range of time and temperature conditions for specimen storage and transport when testing for the presence of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (GC) in urine specimens using the BD ProbeTec ET CT and GC Amplified DNA Assays. In addition, the BD ProbeTec UPT reduces the laboratory processing steps associated with repetitive motion injuries in lab technicians while speeding up the handling of urine samples. Benefits of the BD ProbeTec UPT Kit include: 30-day transport at ambient temperatures (2-30 C) Temperature tolerant during transport (-20 to 60 C) 2-month storage stability at -20 C > 40 % labor reduction compared to the Urine Processing Pouch (UPP) protocol Elimination of 2-hour UPP incubation Low inhibition rates (< 1 %) kit BD ProbeTec ET - Accessories Kit The CT/GC Accessories Kit contains priming plate covers, amplification plate sealers and disposal bags (20 of each per kit). BD Diagnostics Diagnostic Systems Catalog 2008/

216 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for AP Assays tests BD ProbeTec - CF Assay Reagent Pack The Chlamydiaceae Family (CF) Assay, for use with the BD ProbeTec ET System, employs Strand Displacement Amplification (SDA) technology for the direct and qualitative detection of DNA from organisms belonging to the Chlamydiaceae family (including Chlamydia trachomatis, Chlamydophila pneumoniae and Chlamydophila psittaci) from lower respiratory specimens (e.g., sputum, bronchoalveolar lavage, bronchial washing) and throat swabs (with and without transport medium) from patients with a clinical suspicion of pneumonia. The assay is indicated in the presumptive diagnosis of Chlamydiaceae Family associated pneumonia. Pack also includes priming plate covers, amplification plate sealers and disposal bags. Sufficient for 24 tests tests BD ProbeTec - LP Assay Reagent Pack The Legionella pneumophila (LP) Assay, for use with the BD ProbeTec ET System, employs Strand Displacement Amplification (SDA) technology for the direct and qualitative detection of Legionella pneumophila DNA (serogroups 1-14) in sputum specimens from patients with a clinical suspicion of pneumonia. The assay is intended to aid in the presumptive diagnosis of Legionnaires disease in conjunction with culture and other methods. Pack also includes priming plate covers, amplification plate sealers and disposal bags. Sufficient for 24 tests tests BD ProbeTec - MP Assay Reagent Pack The Mycoplasma pneumoniae (MP) Assay, for use with the BD ProbeTec ET System, employs Strand Displacement Amplification (SDA) technology for the direct and qualitative detection of Mycoplasma pneumoniae DNA from lower respiratory specimens (e.g., sputum, bronchoalveolar lavage, bronchial washing) and throat swabs (with and without transport medium) from patients with a clinical suspicion of pneumonia. The assay is indicated as an aid in the presumptive diagnosis of Mycoplasma pneumoniae associated pneumonia. Pack also includes priming plate covers, amplification plate sealers and disposal bags. Sufficient for 24 tests tips controls pcs pcs controls BD ProbeTec ET - Pipette Tips Sterile aerosol resistant pipette tips for the BD ProbeTec ET Pipettor. Total of 720 tips (6 racks of 120). BD ProbeTec ET - Respiratory Diluent and Control Kit Contains 10 positive and 10 negative controls. 50 ml diluent - sufficient for 100 tests BD ProbeTec ET - Sample Tube Caps (4 ml) Replacement caps for the BD ProbeTec ET Sample Tubes. BD ProbeTec ET - Sample Tubes and Caps (4 ml) 4 ml Sample Tubes and Caps that are used for the preparation of urine specimens for an BD ProbeTec ET Assay. BD ProbeTec ET - Swab Diluent and Control Kit Contains 10 positive and 10 negative controls. 50 ml diluent - sufficient for 100 tests 10-8 BD Diagnostics Diagnostic Systems Catalog 2008/2009

217 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for TB Assays tests BD ProbeTec - (DTB) Direct Detection Reagent Pack The BD ProbeTec ET Mycobacterium tuberculosis Complex (DTB) Direct Detection Reagent Pack, for use with the BD ProbeTec ET system, utilizes Strand Displacement Amplification (SDA) technology for the direct qualitative detection of Mycobacterium tuberculosis complex DNA from decontaminated, digested clinical respiratory samples such as sputa, induced sputa, bronchial washings and other respiratory specimens. It is to be used as a direct test for evaluating specimens from untreated patients that are suspected of having tuberculosis. Untreated patients are those who have not received anti-tuberculosis therapy or received such therapy in the last 12 months or had less than 7 days of therapy. The Pack contains 96 tests and also includes priming plate covers, amplification plate sealers and disposal bags controls kit tests BD ProbeTec - (DTB) Direct Detection Control Set Includes 20 positive and 20 negative controls used with the BD ProbeTec ET Mycobacterium tuberculosis Complex (DTB) Direct Detection Reagent Test. One positive and one negative control must be included in each run. BD ProbeTec - (DTB) Direct Detection Specimen Processing Kit Contains buffer solutions for the preparation of 200 specimens. 1 bottle each of DTB wash buffer, DTB lysis buffer and DTB neutralization buffer is included. BD ProbeTec - (KAN) Culture Confirmation Reagent Pack The Mycobacterium kansasii (KAN) Culture Identification Reagent Pack, for use with the BD ProbeTec ET System, utilizes Strand Displacement Amplification (SDA) technology for the identification of Mycobacterium kansasii isolated from culture. Pack also includes priming plate covers, amplification plate sealers and disposal bags tests BD ProbeTec - (CTB) Culture Confirmation Reagent Pack The BD ProbeTec ET Mycobacterium tuberculosis Complex (ctb) culture Identification Test, for use with the BD ProbeTec ET system, utilizes Strand Displacement Amplification (SDA) technology for the identification of Mycobacterium tuberculosis complex DNA from solid or liquid cultures. Pack also includes priming plate covers, amplification plate sealers and disposal bags controls kit units tips units BD ProbeTec - (CTB, KAN) Control Set Includes 20 positive and 20 negative controls used with the BD ProbeTec ET (ctb) and (kan) Culture Identification Tests. One positive and one negative control must be included in each run. BD ProbeTec - (CTB, KAN) Specimen Processing Kit Contains buffer solutions for the preparation of 100 solid or 200 liquid specimens. 1 bottle each of MCI wash buffer, MCI lysis buffer and MCI neutralization buffer is included. BD ProbeTec ET - Accessories II Kit The kit contains 8 each of the priming plate covers, amplification plate sealers and disposal bags. BD ProbeTec ET - Pipette Tips Sterile aerosol resistant pipette tips for the BD ProbeTec ET Pipettor. Total of 720 tips (6 racks of 120). BD ProbeTec ET - Sample Tube Caps (2 ml) Additional caps with a special rubber sealing for the 2 ml Tubes (cat. no ) used for Mycobacteria testing. BD Diagnostics Diagnostic Systems Catalog 2008/

218 Molecular Diagnostics BD ProbeTec ET Consumables BD ProbeTec ET Consumables for TB Assays units BD ProbeTec ET - Sample Tubes and Caps (2 ml) Aerosol resistant tubes for mycobacteria testing with the (DTB), (ctb), (kan) and (mac) Reagent Packs for the BD ProbeTec ET system. Contains 200 tubes and 200 caps with a special rubber sealing BD Diagnostics Diagnostic Systems Catalog 2008/2009

219 Molecular Diagnostics BD Affirm VP III Microbial Identification System BD Affirm VP III Instrument BD MicroProbe Processor (BD Affirm VP III Instrument 220/240 Volt) The BD MicroProbe Processor (220/240 Volt) along with the BD Affirm VP III Microbial Identification Kit is a hybridization based DNA probe test system that identifies Candida spp., Gardnerella vaginalis and Trichomonas vaginalis, which are the major causes of vaginitis/bacterial vaginosis (BV) from a single vaginal sample. Total time-to-result is less than one hour and the instrument can simultaneously run up to 6 specimens. The instrument includes a lysis block and program card BD Affirm VP III - Workstation The BD Affirm VP III Workstation is a molded plastic tray used to ease the set-up and handling of the different reagents of the BD Affirm VPIII Microbial Identification Kit. BD Affirm VP III Consumables BD Affirm VP III - Ambient Temperature Transport System The BD Affirm VP III Ambient Temperature Transport System (ATTS) is a sterile, ready to use system intended for the collection, transport and preservation of vaginal specimens and is only for use with the BD Affirm VP III Microbial Identification Test. This ATTS should be used with those specimens where transport times are expected to exceed 1 h at ambient temperature or 4 h at refrigerated temperatures. The BD Affirm VP III Ambient Temperature Transport System is designed to stabilize the nucleic acid of Candida species, Gardnerella vaginalis, and Trichomonas vaginalis during specimen transport at ambient transport (15-30 C) for up to 72 h units tests BD Affirm VP III - Amies Transport with Swabs Tubes with transport medium and swab. Contains 100 tubes and swabs. BD Affirm VP III - Microbial Identification Kit The BD Affirm VP III Microbial Identification Test is a DNA probe-based test for the simultaneous, differential detection and identification of Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis. The kit includes 24 tests. Material provided: 24 Probe Analysis Cards (PAC), 24 reagent cassettes, 1 bottle of substrate solution, 1 bottle of lysis solution, 1 bottle of buffer solution & 24 filter tips sets BD Affirm VP III - Sample Collection Kit BD Diagnostics Diagnostic Systems Catalog 2008/

220 Molecular Diagnostics BD Diagnostics GeneOhm MRSA (Methicillin-resistant Staphylococcus aureus), GBS (Group B Streptokoccus), VRE (Vancomycin-resistant Enterokocci), MRSA / MSSA (Methicillin-sensitive Staphylococcus aureus) Kits tests tests tests tests tests tests tests tests BD GeneOhm - MRSA Assay BD GeneOhm MRSA assay is a qualitative in vitro diagnostic test for the direct detection of nasal colonization by Methicillin resistant Staphylococcus aureus (MRSA). The real time PCR test is performed on the SmartCycler instrument with a nasal swab specimen from patients at risk for colonization. BD GeneOhm - StaphSR Kit - MRSA / MSSA BD GeneOhm StaphSR is a qualitative in vitro test for quick detection of Methicillin resistant Staphylococcus aureus (MRSA) and Methicillin sensitive Staphylococcus aureus (MSSA). The real time PCR test is performed on the SmartCycler instrument. BD GeneOhm - StrepB Assay BD GeneOhm StrepB assay is a qualitative in vitro diagnostic test for the rapid detection of Group B Streptococcus (GBS) DNA in vaginal/rectal specimens. The real time PCR test is performed on the SmartCycler. BD GeneOhm - IDI-Lysis Kit BD GeneOhm Lysis kit is for the rapid lysis of cells and spores from different sample types. BD GeneOhm - VanR Kit - Vancomycin-resistant Enterokocci (VRE) BD GeneOhm VanR is a qualitative in vitro test for rapid detection and identification of Vancomycin resistant Enterococci (VRE) directly with rectal specimens (rectal swabs, stool samples). The real time PCR test is performed on the SmartCycler. Accessories sets sets BD GeneOhm - MRSA DNA RUO MRSA DNA samples for training purposes (negative sample, weak positive sample, positive sample) BD GeneOhm - Strep B DNA RUO Strep B DNA samples for training purposes (negative sample, weak positive sample, positive sample) BD Diagnostics Diagnostic Systems Catalog 2008/2009

221 Data Management, Statistics & Epidemiology BD EpiCenter Data Management System (an optional component) is a unique solution for microbiology laboratories to communicate and track all the information needed by every professional involved in the diagnosis of infectious diseases, the monitoring of bacterial epidemiology, resistance, and patient treatment. BD EpiCenter TM Advance Data Management System 11-2 BD EpiCenter TM for BD Phoenix TM 11-4 BD EpiCenter TM for BD BACTEC 9000 TM Series 11-5 BD EpiCenter TM for BD BACTEC TM MGIT TM

222 Data Management, Statistics & Epidemiology BD EpiCenter Advanced Data Management System Available as BD EpiCenter Ready To Use (RTU) and BD EpiCenter PLUS. BD EpiCenter Advanced Data Management System For the BD Phoenix System, for the BD BACTEC MGIT 960 System and for the BD BACTEC 9000 Series. 1 The Flexible Solution for Biological Validation, Epidemiology & Nosocomial Infection Control The BD EpiCenter Data Management System integrates modern data visualization components and data integrity rules into an effective data mining tool for examining and analyzing data from BD Phoenix, BD BACTEC MGIT 960, and the BD BACTEC 9120/9240 Blood Culture Systems as well as manual test results. The BD EpiCenter System is a companion workstation that provides microbiology laboratories with workflow management, patient demographic storage, advanced query and statistical support, and custom reporting. The RTU (ready to use) version meets the major needs of a clinical microbiology laboratory by providing basic customization and configuration options. The PLUS version provides expanded flexibility in terms of configuration, customization, data mining, and results interpretation. As of Version 5.5 the BD EpiCenter System optionally features the barcode printing, BD Sentinel, and TB-eXiST * modules. For a detailed description please refer to the separate descriptions of the modules. * trademark pending BD EpiCenter - Epidemiology Package Part of the BD EpiCenter PLUS System Configuration providing a large set of filters and reports on trendings of organism incidence, resistance markers, phenotypes, susceptibility pattern, and MIC distribution. BD EpiCenter - Surveillance Package Part of the BD EpiCenter PLUS System Configuration which offers surveillance filters and reports to monitor phenotypes on the levels of isolates, patients and services, and to track nosocomial infections. BD EpiCenter PLUS - Activation Module Enhancing module for the EpiCenter RTU Data Management System. Includes the Epidemiology and Surveillance packages as well as additional filter and reports. Users can configure their own manual tests, new filters, and new reports. A bidirectional LIS interface is also included. BD EpiCenter - Multi-User Module Client-server application (multiuser functionality) which allows users to connect several BD EpiCenter workstations to one central BD EpiCenter server, thus making it absolutely flexible in terms of accessing data. For instance, it enables microbiologists to remotely control instrument results, review patient results and statistics from the office. It can also be configured to provide direct, real-time data access for infection control specialists, clinicians or other associates. BD EpiCenter EpiCare (Clinical Application Rules Editor) - Open Expert System Module BD EpiCARE is an open expert system which allows you to develop your own rules to ensure more reliable test results according to your laboratory practices, specific requirements and local guidelines and to track and alert in real time when specific isolates detected require focus. It provides microbiologists a tool to author their own set of expert rules with an intuitive user interface. For example, one can suppress a drug, change a susceptibility interpretation, detect a specific phenotype and generate an alert. Furthermore, it features the possibility to test/debug newly created rules before using them on clinical results BD Diagnostics Diagnostic Systems Catalog 2008/2009

223 Data Management, Statistics & Epidemiology BD EpiCenter Advanced Data Management System Available as BD EpiCenter Ready To Use (RTU) and BD EpiCenter PLUS BD EpiCenter Advanced Data Management System For the BD Phoenix System, for the BD BACTEC MGIT 960 System and for the BD BACTEC 9000 Series. BD EpiCenter Sentinel The BD Sentinel module features advanced message distribution functionality within the lab (i.e. microbiologist, lab manager) and between lab and hospital institutions (i.e. wards, clinicians, epidemiologist, infectiologist) including optional network report printing, messaging and forwarding system alerts. Cat.No BD EpiCenter Barcode Printing Module Barcode printing module (cat. no ) allows to print customized barcodes on a barcode printer. Barcodes can also be printed on the Specimen Lab Report. The Barcode Printer Kit (cat.no ) prints barcodes with the patient name, accession number, and drug name, and concentration. BD EpiCenter for BD Phoenix 1 BD EpiCenter Data Management for BD Phoenix The field of clinical microbiology is becoming increasingly complex due to the volume of information that is handled, the emergence of new practices and of a growing number of drugresistant organisms, and the importance of examining and tracking the epidemiology of infectious diseases within your hospital, health-care system, or community. Within this context, BD EpiCenter is the ideal clinical data management system designed for use with BD Phoenix instrument systems. It provides your microbiology laboratory with workflow management, patient demographic storage, advanced query and reporting for data generated by BD Phoenix instrument systems and completed by other microbiology tests. BD EpiCenter is an effective data-mining tool designed to assist you in examining the epidemiology of infectious diseases within your microbiology laboratory and in improving patient care. BD EpiCenter incorporates the BDXpert system which enforces integrity of the result data. Modern reporting tools make the BD EpiCenter system an easy and effective way to evaluate and share complex data generated in the clinical microbiology laboratory BD EpiCenter - Workstation BD EpiCenter - 17 inch Monitor BD EpiCenter - Starter Kit Includes: Network cables, ties, power cord, micro hub, bar code gun, security key, DVD discs, DVD rack, Software, card NIC 3 Com BD EpiCenter - Data Management System, Software Ready to use version. BD Diagnostics Diagnostic Systems Catalog 2008/

224 Data Management, Statistics & Epidemiology BD EpiCenter Advanced Data Management System Available as BD EpiCenter Ready To Use (RTU) and BD EpiCenter PLUS. BD EpiCenter for BD BACTEC BD EpiCenter Data Management for BD BACTEC 9000 series The BD EpiCenter is the ideal clinical data management solution for use with BD BACTEC 9000 Blood Culture System. It is a companion workstation that, through modular design, is easily configured to meet the every day operational needs of your microbiology laboratory and improve your workflow. As the number of tests performed by your microbiology laboratory can increase, your needs for data management can change. Therefore, the BD EpiCenter is designed to connect additional BD BACTEC instruments in a plug-and-play manner to cope constantly with your needs. BD EpiCenter is designed to store additional test results when a positive vial is detected. These results can either be entered manually (e.g. Gram Stain), come from other BD instruments (e.g. Identification) or come from your Laboratory Information System (LIS). BD EpiCenter easily connects to your LIS to exchange and combine patient data results in real time, thereby enhancing safety, patient management and optimizing your work processes BD EpiCenter - Workstation BD EpiCenter - 17 inch Monitor BD EpiCenter - Starter Kit Includes: Network cables, ties, power cord, micro hub, bar code gun, security key, DVD discs, DVD rack, Software, card NIC 3 Com BD EpiCenter - Data Management System, Software Ready to use version. BD EpiCenter - UTP Cable (20 m) UTP Cable 20 m. BD EpiCenter - 3Com Etherlink III Card BD EpiCenter - Lantastic Software 11-4 BD Diagnostics Diagnostic Systems Catalog 2008/2009

225 Data Management, Statistics & Epidemiology BD EpiCenter Advanced Data Management System Available as BD EpiCenter Ready To Use (RTU) and BD EpiCenter PLUS. BD EpiCenter for BD BACTEC MGIT BD EpiCenter Data Management for BD BACTEC MGIT 960 BD EpiCenter system is the ideal clinical data management system designed for use with BD BACTEC MGIT 960 System instruments and your Laboratory Information System (LIS). It is a companion workstation that is easily configured to meet the every day operational needs of your mycobacteriology laboratory. It provides you with bar code scanning and real time LIS communication capabilities, thereby enhancing safety and optimizing your work processes. Its long-term database consolidates data generated either by your BD instruments (Growth and Detection MGIT tubes, Antimicrobial Susceptibility Test sets), your LIS or manually entered data (e.g. Acid Fast Smear result when a Growth and Detection MGIT tube is detected positive). It includes a modern graphical user interface tool to examine and validate your mycobacteria data, track susceptibility patterns and ensure better follow up and improved patient care BD EpiCenter - Workstation BD EpiCenter - 17 inch Monitor BD EpiCenter - Starter Kit Includes: Network cables, ties, power cord, micro hub, bar code gun, security key, DVD discs, DVD rack, Software, card NIC 3 Com BD EpiCenter - Data Management System, Software Ready to use version. BD Diagnostics Diagnostic Systems Catalog 2008/

226

227 Indices Alphabetical Indes 12-2 Numerical Index 12-28

228 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media 1 10 % Potassium Hydroxide YT Medium (Yeast Extract Tryptone) 2-39 A A-1 Medium 2-3 Acetamide Agar 3-18 Acetate Differential Agar 2-3 Acid Fast Bacilli (AFB) Quality Control Slide 6-24 Acid Fast Bacilli (AFB) Quality Control Slide 9-4 Acridine Orange 6-14 Acridine Orange Stain 6-24 Acridine Orange Stain 9-4 Actinomycete Isolation Agar 2-3 Adjuvant, Complete (Freund) 6-13 Adjuvant, Complete H37 Ra 6-13 Adjuvant, Incomplete (Freund) 6-13 Adonitol 2-42 Aeromonas Yersinia Agar 3-2 Agar (Grade A) 2-2 Agar (Granulated) 2-2 Agar (Noble) 2-2 Agar (Purified) 2-2 Agar (Technical) 2-2 Agarose 2-2 AK Agar No. 2 (Sporulating Agar) 2-3 Alkalescens-Dispar Antiserum Poly 6-7 Alkaline Peptone Water 3-18 Anaerobe Broth MIC 2-3 Anaerobic Agar 2-3 Antibiotic Medium Antibiotic Medium Antibiotic Medium Antibiotic Medium Antibiotic Medium 11 (ph 8) 3-28 Antibiotic Medium Antibiotic Medium Antibiotic Medium A Antibiotic Medium Antibiotic Medium Antibiotic Medium Antibiotic Medium Antibiotic Medium Antibiotic Medium Antimicrobic Vial A 2-33 Antimicrobic Vial K 2-33 Antimicrobic Vial P 2-33 APT Agar 2-4 APT Broth 2-4 Asparagine 2-42 Azide Blood Agar Base 2-4 Azide Dextrose Broth 2-4 B B12 Assay Medium 2-4 B12 Culture Agar 2-4 B12 Inoculum Broth 2-4 Bacteroides Bile Esculin Agar with Amikacin (BBE Agar) 3-2 Baird-Parker Agar 3-2 Baird-Parker Agar Base 2-4 BBL Fluid Thioglycollate Medium 3-25 BBL mei Agar (60x15mm) 3-17 BCYE Agar 3-2 BCYE Agar Base 2-4 BCYE Agar with Vancomycin and Colistin 3-2 BCYE Agar without Cysteine with Pimaricin 3-2 BD Affirm VP III - Ambient Temperature Transport System BD Affirm VP III - Amies Transport with Swabs BD Affirm VP III - Microbial Identification Kit BD Affirm VP III - Sample Collection Kit BD Affirm VP III - Workstation BD BACTEC - Barcode Labels 8-6 BD BACTEC - BD FOS Culture Supplement Kit 8-4 BD BACTEC - Blood Culture System 8-2 BD BACTEC - Bottle Holder 8-6 BD BACTEC - Lamps BD Diagnostics Diagnostic Systems Catalog 2008/2009

229 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD BACTEC - Lytic/10 Anaerobic/F Medium 8-3 BD BACTEC - MicroMGIT Calibrator 9-10 BD BACTEC - MicroMGIT Fluorescence Reader 9-10 BD BACTEC - Myco/F Lytic Medium 8-3 BD BACTEC - Mycosis IC/F Medium Culture Vials 8-3 BD BACTEC - Peds Plus Medium 8-3 BD BACTEC - Plug 8-6 BD BACTEC - Standard Anaerobic/F Medium 8-4 BD BACTEC - Standard/10 Aerobic/F Medium 8-4 BD BACTEC - Stands 8-6 BD BACTEC - Temperature Test Vials 8-6 BD BACTEC - Transport box for Blood Culture Bottles 8-6 BD BACTEC - Vial Tray 8-6 BD BACTEC MGIT ml tube Spare Barcode Labels 9-13 BD BACTEC MGIT AST Carrier Set (Spare Barcodes) BD BACTEC MGIT AST Carrier Sets (2-, 3-, 4-, 5-, and 8-tube) BD BACTEC MGIT AST Starter Kit 9-13 BD BACTEC MGIT AST Transport Rack 9-13 BD BACTEC MGIT Calibrators Kit 9-11 BD BACTEC MGIT EMB 7.5 Kit 9-12 BD BACTEC MGIT INH 0.4 Kit 9-12 BD BACTEC MGIT IR Kit 9-12 BD BACTEC MGIT Mycobacterial Detection Instrument 9-11 BD BACTEC MGIT Plug, Bad Station 9-13 BD BACTEC MGIT Positive Tubes 9-11 BD BACTEC MGIT PZA Kit 9-12 BD BACTEC MGIT PZA Medium 9-12 BD BACTEC MGIT SIRE Kit 9-12 BD BACTEC MGIT STR 4.0 Kit 9-12 BD BACTEC MGIT Supplement Kit 9-11 BD BACTEC MGIT Temperature Control Tube 9-11 BD BACTEC MGIT Tubes 7 ml 9-11 BD BACTEC PLUS - Aerobic/F Medium 8-3 BD BACTEC PLUS - Anaerobic/F Medium 8-3 Enterotube II - Codebook 7-6 Go Slide 3-32 Prompt Inoculation System 7-2 B Urotube 3-32 Urotube - Bactrim SXT 3-32 Urotube - E 3-32 Urotube - E. coli 3-32 Urotube - M 3-32 BD Bio-Bag Type C 4-6 BD CampyPak Plus - Disposable Hydrogen + Carbon Dioxide Generator Envelope 4-3 BD Cefinase Paper Disc (¼") 7-9 BD CHROMagar Candida 3-17 BD CHROMagar Listeria (100 mm) 3-17 BD CHROMagar MRSA 3-17 BD CHROMagar O BD CHROMagar Orientation 3-17 BD CHROMagar Orientation // Columbia CNA Agar 3-15 BD CHROMagar Salmonella 3-17 BD CHROMagar Salmonella // XLD Agar 3-15 BD CHROMagar Staph aureus 3-17 BD ColorPAC - Toxin A Test Kit 5-6 BD CrystalSpec - Standards 7-5 BD Crystal - Anaerobe ID Kit 7-3 BD Crystal - Anaerobe Manual Codebook 7-5 BD Crystal - AutoReader 7-5 BD Crystal - Color Reaction Charts 7-5 BD Crystal - Enteric/Nonfermenter ID Kit 7-3 BD Crystal - Enteric/Stool ID Inoculum Fluid 7-4 BD Crystal - Gram-Positive ID Kit 7-3 BD Crystal - Inoculum Fluid 7-4 BD Crystal - MIND Software 7-5 BD Crystal - Neisseria/Haemophilus ID Kit 7-3 BD Crystal - Panel Viewer 7-5 BD Crystal - Panel Viewer Longwave UV Tube 7-5 BD Crystal - Panel Viewer White Light Tube 7-5 BD Crystal - Rapid Gram-Positive ID Kit 7-4 BD Crystal - Reference Panel 7-5 BD CTA Medium 2-8 BD CTA Medium 3-19 BD CultureSwab - Cary-Blair Agar (Single Swab) 1-2 BD Diagnostics Diagnostic Systems Catalog 2008/

230 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD CultureSwab - Liquid Amies (Single Swab) 1-2 BD CultureSwab - Liquid Stuart (Double Swab) 1-2 BD CultureSwab - Liquid Stuart (Mini Tip Swab) 1-2 BD CultureSwab - Liquid Stuart (Single Swab) 1-2 BD CultureSwab - Sterile (Single Swab) 1-2 BD CultureSwab EZ - Sterile (Double Swab) 1-2 BD CultureSwab EZ - Sterile (Single Swab) 1-2 BD CultureSwab PLUS - Amies Gel with Charcoal (Single Swab) BD CultureSwab PLUS - Amies Gel without Charcoal (Single Swab) BD Directigen - Flu A Test Kit 5-8 BD Directigen - Flu A+B Test Kit 5-7 BD Directigen - Group B Strep Test Kit 5-5 BD Directigen - H. influenzae type B Test Kit 5-3 BD Directigen - Meningitis Combo Test Cards 5-3 BD Directigen - Meningitis Combo Test Kit 5-3 BD Directigen - Meningitis Specimen Buffer 5-3 BD Directigen - N. meningitidis Group B/E. coli K1 Test Kit 5-3 BD Directigen - N. meningitidis Groups A, C, Y and W135 Test Kit 5-4 BD Directigen - RSV Test Kit 5-8 BD Directigen - RSV Test Kit 5-9 BD Directigen - S. pneumoniae Test Kit 5-4 BD Directigen EZ - Flu A+B 5-7 BD Directigen EZ - RSV Test Kit 5-8 BD Dispenstirs Device 5-12 BD DrySlide - Indole 7-6 BD DrySlide - Nitrocefin 7-6 BD DrySlide - Oxidase 7-6 B BD EpiCenter - 17 inch Monitor 11-6 BD EpiCenter - 3Com Etherlink III Card 11-5 BD EpiCenter - Data Management System, Software 11-5 BD EpiCenter - Data Management System, Software 11-6 BD EpiCenter - Data Management System, Software 11-4 BD EpiCenter - Epidemiology Package 11-2 BD EpiCenter - Lantastic Software 11-5 BD EpiCenter - Multi-User Module 11-2 BD EpiCenter - Starter Kit 11-4 BD EpiCenter - Starter Kit 11-5 BD EpiCenter - Starter Kit 11-6 BD EpiCenter - Surveillance Package 11-2 BD EpiCenter - UTP Cable (20 m) 11-5 BD EpiCenter - Workstation 11-4 BD EpiCenter - Workstation 11-5 BD EpiCenter - Workstation 11-6 BD EpiCenter Barcode Printing Module 11-3 BD EpiCenter Data Management for BD BACTEC 9000 series BD EpiCenter Data Management for BD BACTEC MGIT BD EpiCenter Data Management for BD Phoenix 11-4 BD EpiCenter EpiCare (Clinical Application Rules Editor) - Open Expert System Module 11-2 BD EpiCenter PLUS - Activation Module 11-2 BD EpiCenter Sentinel 11-3 BD EpiCenter TB-eXiST 11-3 BD EpiCenter TB-eXiST 11-6 BD Falcon - Sputum Collection Kit 1-5 BD Falcon - Sputum Collection Kit 9-2 BD DrySlide - PYR Kit 7-6 BD Enterococcosel Agar 2-10 BD Enterococcosel Agar 3-19 BD Enterococcosel Agar 3-6 BD Enterococcosel Agar with Vancomycin (8μg/ml) 3-6 BD Enterococcosel Broth 2-10 BD Enterococcosel Broth 3-19 BD Falcon SWUBE - 15 cm, Double Cotton Swab (Screw Cap) BD Falcon SWUBE - 15 cm, Single Cotton Swab (Friction Cap) BD Falcon SWUBE - 15 cm, Single Cotton Swab (Screw Cap) BD Falcon SWUBE - 15 cm, Single Polyester Swab (Friction Cap) BD Falcon SWUBE - 15 cm, Single Polyester Swab (Screw Cap) BD Enterotube II 7-6 BD EpiCenter - 17 inch Monitor 11-4 BD EpiCenter - 17 inch Monitor 11-5 BD GasPak - CO2 Indicator Strips 4-3 BD GasPak - Dry Anaerobic Indicator Strips 4-3 BD GasPak "O" Ring Gasket BD Diagnostics Diagnostic Systems Catalog 2008/2009

231 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD GasPak Holding Jar 4-4 BD GasPak Holding Jar Lid 4-4 BD GasPak Improved Clamp/Thumbscrew Assembly 4-5 BD GasPak Lid 4-4 BD GasPak Lid without O-Ring 4-4 BD GasPak Polycarbonate Jar without Lid 4-4 BD GasPak System Complete 4-4 BD GasPak System Complete (Vented) 4-4 BD GasPak Vented Lid (Complete) 4-4 BD GasPak 100 and Tube Holder 4-5 BD GasPak Anaerobic Jar without Lid (Large) 4-4 BD GasPak Anaerobic System Complete (Large) 4-4 BD GasPak Anaerobic System Complete (Vented, Large) 4-4 BD GasPak Inner Lid 4-4 BD GasPak Lid Assembly 4-4 BD GasPak Rack 4-5 BD GasPak EZ - Anaerobe Container System Sachets 4-2 BD GasPak EZ - Anaerobe Pouch System 4-2 BD GasPak EZ - BD CampyPouch System 4-2 BD GasPak EZ - Campy Container System Sachets 4-2 BD GasPak EZ - CO2 Container System Sachets 4-2 BD GasPak EZ - CO2 Pouch System 4-2 BD GasPak EZ - Container Rack 4-2 BD GasPak EZ - Incubation Container 4-2 BD GasPak EZ - Sachet Anaerobe with Indicator 4-2 BD GasPak Plus - Bulk Kit 4-3 BD GasPak Plus - Disposable Hydrogen + Carbon Dioxide Generator Envelope 4-3 BD GC-Lect Agar 3-6 BD GeneOhm - IDI-Lysis Kit BD GeneOhm - MRSA Assay BD GeneOhm - MRSA DNA RUO BD GeneOhm - StaphSR Kit - MRSA / MSSA BD GeneOhm - Strep B DNA RUO BD GeneOhm - StrepB Assay BD GeneOhm - VanR Kit - Vancomycin-resistant Enterokocci (VRE) BD GonoGen II 5-4 BD Hycheck - for Detection of Enterobacteriaceae 3-34 B BD Hycheck - for Disinfection Control 3-34 BD Hycheck - for Yeasts and Molds 3-34 BD Hycheck - for Yeasts and Molds with TTC 3-34 BD Hycheck - Plate Count Agar with TTC 3-34 BD Hycheck - Total Count 3-34 BD IsoVitaleX Enrichment 2-34 BD LINK2 - H. Pylori Rapid Test Kit 5-2 BD LINK2 - H. Pylori Rapid Test Quality Control Kit 5-2 BD Macro-Vue RPR - Antigen (Plastic) Dispensing Bottle 5-12 BD Macro-Vue RPR - Card Test Kit No BD Macro-Vue RPR - Card Test Kit No BD Macro-Vue RPR - Card Test Kit No BD Macro-Vue RPR - Card Test Kit No BD Macro-Vue RPR - Control Cards 5-11 BD Macro-Vue RPR - Liquid Controls 5-11 BD Macro-Vue RPR Card - Antigen Suspension 5-12 BD Macro-Vue RPR Card - Brewer Diagnostic 5-12 BD Macro-Vue RPR Card - Test Rotator Accessories Package 5-12 BD Macro-Vue RPR Card - Test Rotator Model BD Macro-Vue RPR Needle (Dispensing) 5-12 BD MGIT - AST SIRE Test Kit (manual) 9-10 BD MGIT - OADC Enrichment 9-10 BD MGIT - PANTA Antibiotic Mixture 9-10 BD MGIT - Tubes (manual, 4 ml) 9-10 BD Microbial Content Test Agar 3-37 BD Microbial Content Test Agar 3-38 BD Microbial Content Test Agar (Gamma-Irradiated) 3-35 BD MicroProbe Processor (BD Affirm VP III Instrument 220/240 Volt) BD Microtrol - Aeromonas hydrophila, ATCC BD Microtrol - Aspergillus niger, ATCC BD Microtrol - Bacillus cereus, ATCC BD Microtrol - Bacillus subtilis, ATCC BD Microtrol - Bacteroides fragilis, ATCC BD Microtrol - Campylobacter jejuni, NCTC BD Microtrol - Candida albicans, ATCC BD Microtrol - Candida albicans, ATCC BD Microtrol - Citrobacter freundii, ATCC BD Diagnostics Diagnostic Systems Catalog 2008/

232 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Microtrol - Clostridium perfringens, ATCC BD Microtrol - Clostridium sporogenes, ATCC BD Microtrol - Enterobacter aerogenes, ATCC BD Microtrol - Enterobacter cloacae, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus faecalis, ATCC BD Microtrol - Enterococcus hirae, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Escherichia coli, ATCC BD Microtrol - Haemophilus influenza, ATCC BD Microtrol - Klebsiella aerogenes, NCTC BD Microtrol - Klebsiella pneumonia, ATCC BD Microtrol - Klebsiella pneumoniae, NCTC BD Microtrol - Listeria monocytogenes, NCTC BD Microtrol - Listeria monocytogenes, NCTC BD Microtrol - Neisseria Gonorrhoeae, NCTC BD Microtrol - Proteus mirabilis, ATCC BD Microtrol - Proteus vulgaris, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Pseudomonas aeruginosa, ATCC BD Microtrol - Salmonella Nottingham, ATCC BD Microtrol - Salmonella poona, NCTC BD Microtrol - Salmonella typhimurium, ATCC BD Microtrol - Serratia marcescens, ATCC BD Microtrol - Staphylococcus aureus (MRSA), NCTC BD Microtrol - Staphylococcus aureus, ATCC BD Microtrol - Staphylococcus aureus, ATCC BD Microtrol - Staphylococcus aureus, ATCC BD Microtrol - Staphylococcus aureus, ATCC 6538 P 7-35 BD Microtrol - Staphylococcus aureus, NCTC BD Microtrol - Staphylococcus epidermidis, ATCC BD Microtrol - Streptococcus agalactiae, ATCC BD Microtrol - Streptococcus pneumoniae, ATCC BD Microtrol - Streptococcus pneumoniae, NCTC B BD Microtrol - Streptococcus pyogenes, ATCC BD Microtrol - Yersinia enterocolitica, ATCC BD Mycophil Agar 2-19 BD Mycophil Agar with Low ph 2-19 BD MycoPrep - Specimen Digestion/Decontamination Kit 9-2 BD Mycosel Agar 2-20 BD Mycosel Agar 3-22 BD Mycosel Agar 3-9 BD Mycoslide 3-33 BD Oxi/Ferm Tube II 7-6 BD PhoenixSpec - Calibration Standards 7-25 BD PhoenixSpec - Calibration Standards 7-5 BD PhoenixSpec - Nephelometer 7-25 BD PhoenixSpec - Nephelometer 7-5 BD Phoenix - 25 μl Hamilton-Pipette 7-24 BD Phoenix - 50 l Hamilton-Pipette 7-24 BD Phoenix - AST Broth 7-33 BD Phoenix - AST Broth 7-25 BD Phoenix - AST Indicator 7-25 BD Phoenix - AST Indicator 7-33 BD Phoenix - AST-S Broth 7-30 BD Phoenix - AST-S Indicator 7-30 BD Phoenix - Enterococci Panel (PMIC/ID-61) 7-26 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-50) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-51) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-52) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-53) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-55) TURKEY 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-57) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-62) 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-63) MIDDLE EAST 7-28 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-64) 7-29 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-65) 7-29 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-69) 7-29 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-70) 7-29 BD Phoenix - Gram Negative Combo Panel (NMIC/ID-71) 7-29 BD Phoenix - Gram Negative ID Panels BD Diagnostics Diagnostic Systems Catalog 2008/2009

233 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Phoenix - Gram Positive Combo Panel (PMIC/ID-51) 7-26 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-53) 7-26 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-55) 7-26 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-57) 7-26 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-58) 7-26 BD Phoenix - Gram Positive Combo Panel (PMIC/ID-64) 7-26 BD Phoenix - Gram Positive ID Panels 7-31 BD Phoenix - ID Broth 7-33 BD Phoenix - ID Broth 7-31 BD Phoenix - ID Broth 7-30 BD Phoenix - ID Broth 7-25 BD Phoenix - Inoculation Station 7-24 BD Phoenix - Low Volume Broth 7-25 BD Phoenix - Low Volume Broth 7-30 BD Phoenix - Low Volume Broth 7-33 BD Phoenix - Panel Caddy 7-24 BD Phoenix - Panel Closure 7-25 BD Phoenix - Pipette Stand 7-25 BD Phoenix - Pipette Tips 7-25 BD Phoenix - Pseudomonas Panel (NMIC/ID-68) 7-29 BD Phoenix - Starter Kit 7-24 BD Phoenix - Streptococci Panel (SMIC-10 PLUS) 7-30 BD Phoenix - Urine Panel (UNMIC/ID-61) 7-29 BD Phoenix - Urine Panel (UNMIC/ID-67) 7-29 BD Phoenix Instrument 7-24 BD Phoenix Table 7-24 BD Phoenix AST Only Panel (NMIC-64) 7-32 BD Phoenix AST Only Panel (NMIC-70) 7-32 BD Phoenix AST Only Panel (NMIC-71) 7-32 BD Phoenix AST Only Panel (PMIC 58) 7-32 BD Phoenix AST Only Panel (PMIC-55) 7-32 BD Phoenix AST Only Panel (PMIC-60) 7-32 BD Phoenix AST Only Panel (PMIC-64) 7-32 BD Phoenix AST Only Panel (PMIC-65) 7-32 BD Phoenix AST Only Panel (UNMIC-53) 7-32 BD Phoenix AST Only Panel (UNMIC-67) 7-33 BD Phoenix Gram Positive Combo Panel (PMIC/ID-65) 7-27 BD Phoenix Staphylococci Panel (PMIC/ID-60) 7-26 B BD Phoenix Streptococci Panel (SMIC/ID-9) 7-30 BD Phytone Yeast Extract Agar 2-22 BD Plastipak 10 ml Luer-Lok Syringe 8-5 BD Pneumoslide - Test for Streptococcus pneumoniae 5-5 BD Port-A-Cul - Transport Jar (Sterile Pack) 1-3 BD Port-A-Cul - Tube 1-3 BD Port-A-Cul - Tube & Swabs (Sterile Pack) 1-3 BD Port-A-Cul - Vial 1-3 BD Port-A-Cul - Vial (Sterile Pack) 1-3 BD ProbeTec - (CTB) Culture Confirmation Reagent Pack 10-9 BD ProbeTec - (CTB, KAN) Control Set 10-9 BD ProbeTec - (CTB, KAN) Specimen Processing Kit 10-9 BD ProbeTec - (DTB) Direct Detection Control Set 10-9 BD ProbeTec - (DTB) Direct Detection Reagent Pack 10-9 BD ProbeTec - (DTB) Direct Detection Specimen Processing Kit 10-9 BD ProbeTec - (KAN) Culture Confirmation Reagent Pack 10-9 BD ProbeTec - CF Assay Reagent Pack 10-8 BD ProbeTec - LP Assay Reagent Pack 10-8 BD ProbeTec - MP Assay Reagent Pack 10-8 BD ProbeTec - Urine Preservatie Transport Kit 10-5 BD ProbeTec - Urine Preservatie Transport Kit 10-7 BD ProbeTec Culturette Direct - Collection and Transport System BD ProbeTec Culturette Direct - Collection and Transport System BD ProbeTec ET - Accessories II Kit 10-9 BD ProbeTec ET - Accessories Kit 10-5 BD ProbeTec ET - Accessories Kit 10-7 BD ProbeTec ET - Collection Kit for Endocervical Specimens "wet swab" BD ProbeTec ET - Collection Kit for Endocervical Specimens "wet swab" BD ProbeTec ET - Collection Kit for Male Urethral Specimens "wet swab" BD ProbeTec ET - Collection Kit for Male Urethral Specimens "wet swab" BD ProbeTec ET - Cover for Lysis Block 10-3 BD ProbeTec ET - CT reagent pack with AC 10-4 BD ProbeTec ET - CT reagent pack without AC 10-4 BD ProbeTec ET - CT/GC Control Set 10-4 BD ProbeTec ET - CT/GC Control Set 10-6 BD Diagnostics Diagnostic Systems Catalog 2008/

234 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD ProbeTec ET - CT/GC Reagent Pack with AC 10-6 BD ProbeTec ET - CT/GC Reagent Pack without AC 10-6 BD ProbeTec ET - Diluent (CT/GC) 10-5 BD ProbeTec ET - Diluent (CT/GC) 10-7 BD ProbeTec ET - Instrument 10-2 BD ProbeTec ET - Lysing Heater (220V) 10-3 BD ProbeTec ET - Lysing Rack for 96 sample tubes 10-3 BD ProbeTec ET - Lysing Thermometer 10-3 BD ProbeTec ET - Oven Filter 10-3 BD ProbeTec ET - Oven Thermometer 10-3 BD ProbeTec ET - Pipette Tips 10-5 BD ProbeTec ET - Pipette Tips 10-7 BD ProbeTec ET - Pipette Tips 10-8 BD ProbeTec ET - Pipette Tips 10-9 BD ProbeTec ET - Pipetting Rack 10-2 BD ProbeTec ET - Pipetting Rack 10-3 BD ProbeTec ET - Pipettor 10-2 BD ProbeTec ET - Pipettor Keypad Cover 10-2 BD ProbeTec ET - Pipettor Power Supply (220V, 50 Hz) 10-2 BD ProbeTec ET - Pipettor Rechargable Batteries 10-2 BD ProbeTec ET - Pipettor Stand 10-2 BD ProbeTec ET - Priming and Warming Heater (220V) 10-2 BD ProbeTec ET - Priming and Warming Heater Thermometer 10-2 BD ProbeTec ET - Respiratory Diluent and Control Kit 10-8 BD ProbeTec ET - Sample Tube Caps (2 ml) 10-9 BD ProbeTec ET - Sample Tube Caps (4 ml) 10-5 BD ProbeTec ET - Sample Tube Caps (4 ml) 10-7 BD ProbeTec ET - Sample Tube Caps (4 ml) 10-8 BD ProbeTec ET - Sample Tube Rack 10-2 BD ProbeTec ET - Sample Tube Rack 10-3 BD ProbeTec ET - Sample Tubes and Caps (2 ml) BD ProbeTec ET - Sample Tubes and Caps (4 ml) 10-5 BD ProbeTec ET - Sample Tubes and Caps (4 ml) 10-7 BD ProbeTec ET - Sample Tubes and Caps (4 ml) 10-8 BD ProbeTec ET - Special Oven (all voltage) 10-2 BD ProbeTec ET - Special Oven (all voltage) 10-3 BD ProbeTec ET - Swab Diluent and Control Kit 10-8 B BD ProbeTec ET - Swab Sample Diluent (CT/GC) 10-5 BD ProbeTec ET - Swab Sample Diluent (CT/GC) 10-7 BD ProbeTec ET - Ultrasonic bath 10-2 BD ProbeTec ET - Ultrasonic bath 10-3 BD ProbeTec Mini-Tip BD Culturette Direct 10-4 BD ProbeTec Mini-Tip BD Culturette Direct 10-6 BD Pseudosel Agar 3-10 BD RODAC - Contact Plate (Non-Sterile) 3-34 BD RODAC Plate-Rack 3-36 BD RODAC Unfilled Petri Dishes (Sterile, 65 mm) 3-35 BD Select APS - LB Broth Base 2-39 BD Select APS - Super Broth Base 2-40 BD Select APS - Super Broth Base 2-41 BD Select APS - Tryptic Soy Broth 2-29 BD Select APS - Tryptic Soy Broth 2-41 BD Select APS - Tryptic Soy Broth (Sterile) 2-29 BD Select APS - Tryptic Soy Broth (Sterile) 2-41 BD Sensi-Disc - 6-Place Dispenser 7-12 BD Sensi-Disc - 8-Place Dispenser 7-12 BD Sensi-Disc - Amdinocillin, 10 μg (AMD-10) 7-13 BD Sensi-Disc - Amikacin, 10 μg (AN-10) 7-20 BD Sensi-Disc - Amikacin, 30 μg (AN-30) 7-13 BD Sensi-Disc - Amikacin, 30 μg (AN-30) 7-20 BD Sensi-Disc - Amoxicillin, 10 μg (AMX-10) 7-13 BD Sensi-Disc - Amoxicillin, 25 μg (AMX-25) 7-20 BD Sensi-Disc - Amoxicillin, 25 μg (AMX-25) 7-13 BD Sensi-Disc - Ampicillin, 10 μg (AM-10) 7-13 BD Sensi-Disc - Ampicillin, 10 μg (AM-10) 7-20 BD Sensi-Disc - Ampicillin, 2μg (AM-2) 7-13 BD Sensi-Disc - Ampicillin, 25 μg (AM-25) 7-13 BD Sensi-Disc - Ampicillin, 25 μg (AM-25) 7-20 BD Sensi-Disc - Ampicillin/Sulbactam (SAM-20) 7-13 BD Sensi-Disc - Augmentin (AMC-3) 7-13 BD Sensi-Disc - Augmentin (AMC-30) 7-13 BD Sensi-Disc - Augmentin (AMC-30) 7-20 BD Sensi-Disc - Azithromycin, 15 μg (AZM-15) 7-13 BD Sensi-Disc - Azlocillin, 30 μg (AZ-30) 7-13 BD Sensi-Disc - Azlocillin, 30 μg (AZ-30) BD Diagnostics Diagnostic Systems Catalog 2008/2009

235 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Sensi-Disc - Azlocillin, 75 μg (AZ-75) 7-13 BD Sensi-Disc - Aztreonam, 30 μg (ATM-30) 7-13 BD Sensi-Disc - Bacitracin, 10 μg (B-10) 7-13 BD Sensi-Disc - Bacitracin, 10 μg (B-10) 7-20 BD Sensi-Disc - Bacitracin, 2 μg (B-2) 7-13 BD Sensi-Disc - Blank Disc Nonsterile 7-20 BD Sensi-Disc - Carbenicillin, 100 μg (CB-100) 7-13 BD Sensi-Disc - Cefaclor, 30 μg (CEC-30) 7-13 BD Sensi-Disc - Cefaclor, 30 μg (CEC-30) 7-20 BD Sensi-Disc - Cefadroxyl, 30 μg (CFR-30) 7-20 BD Sensi-Disc - Cefamandole, 30 μg (MA-30) 7-13 BD Sensi-Disc - Cefazolin, 30 μg (CZ-30) 7-13 BD Sensi-Disc - Cefazolin, 30 μg (CZ-30) 7-20 BD Sensi-Disc - Cefdinir, 5 μg (CDR-5) 7-13 BD Sensi-Disc - Cefepime, 30 μg (FEP-30) 7-13 BD Sensi-Disc - Cefepime, 30 μg (FEP-30) 7-20 BD Sensi-Disc - Cefixime, 10 μg (CFM-10) 7-14 BD Sensi-Disc - Cefixime, 5 μg (CFM-5) 7-14 BD Sensi-Disc - Cefonicid, 30 μg (CID-30) 7-14 BD Sensi-Disc - Cefoperazone, 30 μg (CFP-30) 7-20 BD Sensi-Disc - Cefoperazone, 75 μg (CFP-75) 7-14 BD Sensi-Disc - Cefotaxime 30 μg with Clavulanic Acid 10 μg (CTX-30/CLA-10) 7-14 BD Sensi-Disc - Cefotaxime, 30 μg (CTX-30) 7-14 BD Sensi-Disc - Cefotaxime, 30 μg (CTX-30) 7-20 BD Sensi-Disc - Cefotaxime, 5 μg (CTX-5) 7-14 BD Sensi-Disc - Cefotetan, 30 μg (CTT-30) 7-14 BD Sensi-Disc - Cefotiam, 30 μg (CFT-30) 7-20 BD Sensi-Disc - Cefoxitin, 30 μg (FOX-30) 7-14 BD Sensi-Disc - Cefoxitin, 30 μg (FOX-30) 7-20 BD Sensi-Disc - Cefpirome, 30 μg (CPO-30) 7-14 BD Sensi-Disc - Cefpodoxime, 10 μg (CPD-10) 7-14 BD Sensi-Disc - Cefprozil, 30 μg (CPR-30) 7-14 BD Sensi-Disc - Cefsulodin, 30 μg (CFS-30) 7-20 BD Sensi-Disc - Ceftazidime 30 μg with Clavulanic Acid 20 μg (CAZ-30/CLA-10) 7-14 BD Sensi-Disc - Ceftazidime, 30 μg (CAZ-30) 7-14 BD Sensi-Disc - Ceftazidime, 30 μg (CAZ-30) 7-20 BD Sensi-Disc - Ceftiofur, 30 μg (XNL-30) 7-23 B BD Sensi-Disc - Ceftizoxime, 30 μg (ZOX-30) 7-14 BD Sensi-Disc - Ceftriaxone, 30 μg (CRO-30) 7-14 BD Sensi-Disc - Ceftriaxone, 30 μg (CRO-30) 7-20 BD Sensi-Disc - Cefuroxime, 30 μg (CXM-30) 7-14 BD Sensi-Disc - Cefuroxime, 30 μg (CXM-30) 7-20 BD Sensi-Disc - Cefuroxime, 5 μg (CXM-5) 7-14 BD Sensi-Disc - Cephalexin, 30 μg (CN-30) 7-14 BD Sensi-Disc - Cephalexin, 30 μg (CN-30) 7-20 BD Sensi-Disc - Cephalothin, 30 μg (CF-30) 7-14 BD Sensi-Disc - Cephalotin, 30 μg (CF-30) 7-20 BD Sensi-Disc - Cephradine, 30 μg (CH-30) 7-14 BD Sensi-Disc - Chloramphenicol, 10 μg (C-10) 7-14 BD Sensi-Disc - Chloramphenicol, 30 μg (C-30) 7-15 BD Sensi-Disc - Cinoxacin, 100 μg (CIN-100) 7-15 BD Sensi-Disc - Ciprofloxacin, 1 μg (CIP-1) 7-15 BD Sensi-Disc - Ciprofloxacin, 5 μg (CIP-5) 7-15 BD Sensi-Disc - Ciprofloxacin, 5 μg (CIP-5) 7-20 BD Sensi-Disc - Clarithromycin, 15 μg (CLR-15) 7-15 BD Sensi-Disc - Clarithromycin, 2 μg (CLR-2) 7-15 BD Sensi-Disc - Clindamycin, 10 μg (CC-10) 7-21 BD Sensi-Disc - Clindamycin, 2 μg (CC-2) 7-15 BD Sensi-Disc - Clindamycin, 2 μg (CC-2) 7-21 BD Sensi-Disc - Cloxacillin, 1 μg (CX-1) 7-15 BD Sensi-Disc - Colistin, 10 μg (CL-10) 7-15 BD Sensi-Disc - Colistin, 10 μg (CL-10) 7-21 BD Sensi-Disc - Colistin, 25 μg (CL-25) 7-15 BD Sensi-Disc - Dalfopristin/Quinupristin (Synercid), 15 μg (SYN-15) 7-15 BD Sensi-Disc - Designer Dispenser System 7-12 BD Sensi-Disc - Doxycycline, 30 μg (D-30) 7-15 BD Sensi-Disc - Doxycycline, 30 μg (D-30) 7-21 BD Sensi-Disc - Enoxacin, 10 μg (ENX-10) 7-15 BD Sensi-Disc - Enrofloxacin, 5 μg (ENO-5) 7-23 BD Sensi-Disc - Ertapenem, 10 μg (ETP-10) 7-15 BD Sensi-Disc - Erythromycin, 10 μg (E-10) 7-15 BD Sensi-Disc - Erythromycin, 15 μg (E-15) 7-15 BD Sensi-Disc - Erythromycin, 15 μg (E-15) 7-21 BD Sensi-Disc - Erythromycin, 2 μg (E-2) 7-15 BD Diagnostics Diagnostic Systems Catalog 2008/

236 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Sensi-Disc - Erythromycin, 5 μg (E-5) 7-15 BD Sensi-Disc - Ethambutol, 25 μg (EM-25) 7-23 BD Sensi-Disc - Ethambutol, 25 μg (EM-25) 9-8 BD Sensi-Disc - Ethambutol, 50 μg (EM-50) 7-23 BD Sensi-Disc - Ethambutol, 50 μg (EM-50) 9-8 BD Sensi-Disc - Ethionamide, 25 μg (EA-25) 7-23 BD Sensi-Disc - Ethionamide, 25 μg (EA-25) 9-8 BD Sensi-Disc - Fosfomycin (FF-50) W/GL BD Sensi-Disc - Fosfomycin, 200 μg (FOS-200) 7-15 BD Sensi-Disc - Fosfomycin, 50 μg (FF-50) 7-21 BD Sensi-Disc - Furazolidone, 100 μg (FX-100) 7-15 BD Sensi-Disc - Fusidic Acid, 10 μg (FA-10) 7-15 BD Sensi-Disc - Fusidic Acid, 10 μg (FA-10) 7-21 BD Sensi-Disc - Gatifloxacin, 5 μg (GAT-5) 7-15 BD Sensi-Disc - Gentamicin, 10 μg (GM-10) 7-15 BD Sensi-Disc - Gentamicin, 10 μg (GM-10) 7-21 BD Sensi-Disc - Gentamicin, 120 μg (GM-120) 7-16 BD Sensi-Disc - Imipenem, 10 μg (IPM-10) 7-16 BD Sensi-Disc - Imipenem, 10 μg (IPM-10) 7-21 BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 1 μg (INH- 1) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 1 μg (INH- 1) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 5 μg (INH- 5) BD Sensi-Disc - Isoniazid (Isonicotinyl Hydrazine), 5 μg (INH- 5) BD Sensi-Disc - Kanamycin, 30 μg (K-30) 7-16 BD Sensi-Disc - Kanamycin, 30 μg (K-30) 7-21 BD Sensi-Disc - Levofloxacin, 1 μg (LVL-1) 7-16 BD Sensi-Disc - Levofloxacin, 5 μg (LVL-5) 7-16 BD Sensi-Disc - Lincomycin, 15 μg (L-15) 7-21 BD Sensi-Disc - Lincomycin, 2 μg (L-2) 7-16 BD Sensi-Disc - Linezolid, 10 μg (LZD-10) 7-16 BD Sensi-Disc - Linezolid, 30 μg (LZD-30) 7-16 BD Sensi-Disc - Lomefloxacin, 10 μg (LOM-10) 7-16 BD Sensi-Disc - Loracarbef, 30 μg (LOR-30) 7-16 BD Sensi-Disc - Mecillinam, 10 μg (MEC-10) 7-16 BD Sensi-Disc - Meropenem, 10 μg (MEM-10) 7-16 BD Sensi-Disc - Meropenem, 10 μg (MEM-10) 7-21 B BD Sensi-Disc - Metronidazole Elution Discs 7-16 BD Sensi-Disc - Metronidazole, 5 μg (MET-5) 7-16 BD Sensi-Disc - Metronidazole, 80 μg (MET-80) 7-21 BD Sensi-Disc - Mezlocillin, 30 μg (MZ-30) 7-16 BD Sensi-Disc - Mezlocillin, 30 μg (MZ-30) 7-21 BD Sensi-Disc - Mezlocillin, 75 μg (MZ-75) 7-16 BD Sensi-Disc - Minocycline, 30 μg (MI-30) 7-16 BD Sensi-Disc - Moxalactam, 30 μg (MOX-30) 7-16 BD Sensi-Disc - Moxifloxacin, 5 μg (MXF-5) 7-16 BD Sensi-Disc - Mupirocin, 200 μg (MUP-200) 7-17 BD Sensi-Disc - Mupirocin, 5 μg (MUP-5) 7-17 BD Sensi-Disc - Nafcillin, 1 μg (NF-1) 7-17 BD Sensi-Disc - Nalidixic Acid, 30 μg (NA-30) 7-17 BD Sensi-Disc - Nalidixic Acid, 30 μg (NA-30) 7-21 BD Sensi-Disc - Neomycin, 30 μg (N-30) 7-17 BD Sensi-Disc - Neomycin, 30 μg (N-30) 7-21 BD Sensi-Disc - Neomycin, 5 μg (N-5) 7-17 BD Sensi-Disc - Netilmicin, 10 μg (NET-10) 7-17 BD Sensi-Disc - Netilmicin, 30 μg (NET-30) 7-17 BD Sensi-Disc - Netilmicin, 30 μg (NET-30) 7-21 BD Sensi-Disc - Nitrofurantoin 300 μg + Sulfafiazine 100 μg (FM-300/SD-100) 7-21 BD Sensi-Disc - Nitrofurantoin, 100 μg (FM-100) 7-17 BD Sensi-Disc - Nitrofurantoin, 200 μg (FM-200) 7-17 BD Sensi-Disc - Nitrofurantoin, 300 μg (FM-300) 7-21 BD Sensi-Disc - Nitrofurantoin, 300 μg (FM-300) 7-17 BD Sensi-Disc - Nitrofurantoin, 50 μg (FM-50) 7-17 BD Sensi-Disc - Norfloxacin, 10 μg (NOR-10) 7-17 BD Sensi-Disc - Norfloxacin, 10 μg (NOR-10) 7-21 BD Sensi-Disc - Norfloxacin, 2 μg (NOR-2) 7-17 BD Sensi-Disc - Norfloxacin, 5 μg (NOR-5) 7-17 BD Sensi-Disc - Novobiocin, 30 μg (NB-30) 7-17 BD Sensi-Disc - Novobiocin, 5 μg (NB-5) 7-17 BD Sensi-Disc - Novobiocin, 5 μg (NB-5) 7-21 BD Sensi-Disc - Ofloxacin, 10 μg (OFX-10) 7-21 BD Sensi-Disc - Ofloxacin, 5 μg (OFX-5) 7-17 BD Sensi-Disc - Ofloxacin, 5 μg (OFX-5) 7-21 BD Sensi-Disc - Oleandomycin, 15 μg (OL-15) BD Diagnostics Diagnostic Systems Catalog 2008/2009

237 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Sensi-Disc - Oleandomycin, 15 μg (OL-15) 7-21 BD Sensi-Disc - Oxacillin, 1 μg (OX-1) 7-17 BD Sensi-Disc - Oxacillin, 1 μg (OX-1) 7-22 BD Sensi-Disc - Oxacillin, 5 μg (OX-5) 7-22 BD Sensi-Disc - Oxolinic Acid, 2 μg (OA-2) 7-17 BD Sensi-Disc - Oxytetracycline, 30 μg (T-30) 7-17 BD Sensi-Disc - Pefloxacin, 5 μg (PEF-5) 7-17 BD Sensi-Disc - Penicillin, 1 μg (P-1) 7-17 BD Sensi-Disc - Penicillin, 1.5 μg (P-1.5) 7-18 BD Sensi-Disc - Penicillin, 10 μg (P-10) 7-18 BD Sensi-Disc - Penicillin, 10 μg (P-10) 7-22 BD Sensi-Disc - Penicillin, 2 μg (P-2) 7-18 BD Sensi-Disc - Phenicol, 30 μg (C-30) 7-22 BD Sensi-Disc - Pipemidic Acid, 20 μg (PI-20) 7-22 BD Sensi-Disc - Piperacillin 100 μg + Tazobactam 10 μg (PIP-100/TAZ-10) BD Sensi-Disc - Piperacillin 75 μg + Tazobactam10 μg (PIP- 75/TAZ-10) BD Sensi-Disc - Piperacillin, 100 μg (PIP-100) 7-18 BD Sensi-Disc - Piperacillin, 100 μg (PIP-100) 7-22 BD Sensi-Disc - Piperacillin, 30 μg (PIP-30) 7-22 BD Sensi-Disc - Piperacillin, 75 μg (PIP-75) 7-18 BD Sensi-Disc - Polymyxin B, 300 μg (PB-300) 7-18 BD Sensi-Disc - Polymyxin B, 300 μg (PB-300) 7-22 BD Sensi-Disc - Quinupristin/Dalfopristin (Synercid) 15 μg (SYN-15) 7-18 BD Sensi-Disc - Rifampicin, 2 μg (RA-2) 7-18 BD Sensi-Disc - Rifampicin, 30 μg (RA-30) 7-18 BD Sensi-Disc - Rifampin, 25 μg (RA-25) 7-23 BD Sensi-Disc - Rifampin, 25 μg (RA-25) 9-8 BD Sensi-Disc - Rifampin, 5 μg (RA-5) 9-8 BD Sensi-Disc - Rifampin, 5 μg (RA-5) 7-23 BD Sensi-Disc - Rokitamycin, 30 μg (ROK-30) 7-18 BD Sensi-Disc - Single Disc Dispenser 7-12 BD Sensi-Disc - Spectinomycin, 100 μg (SPT-100) 7-18 BD Sensi-Disc - Spiramicin, 100 μg (SP-100) 7-18 BD Sensi-Disc - Streptomycin, 10 μg (S-10) 7-23 BD Sensi-Disc - Streptomycin, 10 μg (S-10) 9-8 BD Sensi-Disc - Streptomycin, 300 μg (S-300) 7-23 BD Sensi-Disc - Streptomycin, 50 μg (S-50) 7-23 B BD Sensi-Disc - Streptomycin, 50 μg (S-50) 9-8 BD Sensi-Disc - Sulbactam 10 μg with Ampicillin 20 μg (SAM-30) BD Sensi-Disc - Sulbactam 30 μg with Cefoperazone 75 μg (SCP 105) BD Sensi-Disc - Sulfadiazine, 0.25 μg (SD-0.25) 7-18 BD Sensi-Disc - Sulfamethoxazole, 23,75 μg (SMZ-23,75) 7-22 BD Sensi-Disc - Sulfathiazole, 0.25 μg (ST-0.25) 7-18 BD Sensi-Disc - Sulfisoxazole, 25 μg (G-25) 7-18 BD Sensi-Disc - Teicoplanin, 30 μg (TEC-30) 7-18 BD Sensi-Disc - Telithromycin, 15 μg (TEL-15) 7-18 BD Sensi-Disc - Temocillin, 30 μg (TEM-30) 7-18 BD Sensi-Disc - Tetracycline, 10 μg (TE-10) 7-18 BD Sensi-Disc - Tetracycline, 30 μg (TE-30) 7-18 BD Sensi-Disc - Tetracycline, 30 μg (TE-30) 7-22 BD Sensi-Disc - Tetracycline, 5 μg (TE-5) 7-18 BD Sensi-Disc - Ticarcillin with Clavulanic Acid (Timentin), 85 μg (TIM-85) 7-19 BD Sensi-Disc - Ticarcillin, 75 μg (TIC-75) 7-18 BD Sensi-Disc - Tobramycin, 10 μg (NN-10) 7-19 BD Sensi-Disc - Tobramycin, 10 μg (NN-10) 7-22 BD Sensi-Disc - Tobramycin, 30 μg (NN-30) 7-22 BD Sensi-Disc - Trimethoprim 1.25 μg + Sulfamethoxazol μg (Cotrimoxazol) (SXT) BD Sensi-Disc - Trimethoprim 1.25 μg + Sulfamethoxazol μg (Cotrimoxazol) (SXT) BD Sensi-Disc - Trimethoprim, 1.25 μg (TR-1.25) 7-19 BD Sensi-Disc - Trimethoprim, 2.5 μg (TR-2.5) 7-19 BD Sensi-Disc - Trimethoprim, 5 μg (TMP-5) 7-19 BD Sensi-Disc - Trimethoprim, 5 μg (TMP-5) 7-22 BD Sensi-Disc - Triple Sulfa, 25 μg (SSS-25) 7-19 BD Sensi-Disc - Vancomycin, 30 μg (VA-30) 7-19 BD Sensi-Disc - Vancomycin, 30 μg (VA-30) 7-22 BD Sensi-Disc - Vancomycin, 5 μg (VA-5) 7-19 BD Staphyloslide - Latex Test Kit 5-4 BD Sterile Pack Swabs 1-6 BD Streptocard - Acid Extraction Reagent 5-5 BD Streptocard - Acid Latex Test Kit 5-5 BD Streptocard - Enzyme Extraction Reagent 5-5 BD Streptocard - Enzyme Latex Test Kit 5-5 BD Streptocard - Group A Latex Reagent 5-5 BD Diagnostics Diagnostic Systems Catalog 2008/

238 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Streptocard - Group B Latex Reagent 5-5 BD Streptocard - Group D Latex Reagent 5-5 BD Streptocard - Positive Control 5-5 BD Streptocard - Reaction Cards 5-5 BD Taxo - A Discs 7-9 BD Taxo - Adonitol Discs 7-8 BD Taxo - ALA Discs 7-9 BD Taxo - Anaerobe Disc Set 7-7 BD Taxo - Arabinose Discs 7-8 BD Taxo - Blank Discs 7-7 BD Taxo - Dextrose Discs 7-8 BD Taxo - Dulcitol Discs 7-8 BD Taxo - Galactose Discs 7-8 BD Taxo - Hippurate Discs 7-9 BD Taxo - INH Test Control 9-9 BD Taxo - INH Test Strips 9-9 BD Taxo - Inositol Discs 7-8 BD Taxo - Kanamycin Discs 7-9 BD Taxo - Lactose Discs 7-8 BD Taxo - Maltose Discs 7-8 BD Taxo - Mannitol Discs 7-8 BD Taxo - N Discs 7-9 BD Taxo - Nitrate Discs 7-9 BD Taxo - Nitrite Test Strips 9-9 BD Taxo - Novobiocin Discs 7-9 BD Taxo - ONPG Discs 7-10 BD Taxo - P Discs 7-10 BD Taxo - Raffinose Discs 7-8 BD Taxo - Rhamnose Discs 7-8 BD Taxo - Sorbitol Discs 7-8 BD Taxo - SPS Discs 7-10 BD Taxo - Sucrose Discs 7-8 BD Taxo - TB Niacin Test Control 9-9 BD Taxo - TB Niacin Test Strips 9-9 BD Taxo - Trehalose Discs 7-8 BD Taxo - Urea Discs 7-10 BD Taxo - V Factor Discs 7-10 BD Taxo - V Factor Strips 7-10 B BD Taxo - X and V Factor Discs 7-10 BD Taxo - X and V Factor Strips 7-11 BD Taxo - X Factor Discs 7-10 BD Taxo - X Factor Strips 7-11 BD Trichosel Broth, Modified 2-28 BD Trichosel Broth, Modified with 5 % Horse Serum 3-26 BD Tryptic Soy Agar 3-36 BD Tryptic Soy Agar 3-37 BD Tryptic Soy Broth 3-40 BD Trypticase Soy Agar 2-29 BD Trypticase Soy Agar 3-12 BD Trypticase Soy Agar 3-14 BD Trypticase Soy Agar 3-26 BD Trypticase Soy Agar 3-36 BD Trypticase Soy Agar 3-37 BD Trypticase Soy Agar 3-38 BD Trypticase Soy Agar 3-40 BD Trypticase Soy Agar - Contact Plates 3-34 BD Trypticase Soy Agar with 5 % Horse Blood (TSA II) 3-12 BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) 3-12 BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) // MacConkey II Agar BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) // MacConkey II Agar with MUG BD Trypticase Soy Agar with Defibrinated Sheep Blood (Blood Agar Slants) BD Trypticase Soy Agar with Lecithin and Polysorbate BD Trypticase Soy Agar with Lecithin and Polysorbate BD Trypticase Soy Agar with Lecithin and Polysorbate BD Trypticase Soy Agar with Lecithin and Polysorbate BD Trypticase Soy Agar with Lecithin and Polysorbate 80 - Contact Plates BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Histidin (Contact Slide) BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicillinase BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicillinase BD Trypticase Soy Agar with Lecithin, Polysorbate 80 and Penicinillase BD Trypticase Soy Agar with Penicillinase 3-36 BD Trypticase Soy Agar, Modified (TSA II) 3-26 BD Trypticase Soy Agar, Modified (TSA II) BD Diagnostics Diagnostic Systems Catalog 2008/2009

239 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B BD Trypticase Soy Broth 2-29 BD Trypticase Soy Broth 3-27 BD Trypticase Soy Broth 3-31 BD Trypticase Soy Broth 3-40 BD Trypticase Soy Broth (Double Strength) 3-41 BD Trypticase Soy Broth (ETO sterile) 3-41 BD Trypticase Soy Broth (Serum Bags) 3-40 BD Trypticase Soy Broth (Sterile Pack Bottle) 3-41 BD Trypticase Soy Broth (Sterile) 2-29 BD Trypticase Soy Broth with 1 % Tween 3-41 BD Trypticase Soy Broth with 20 % Glycerol 3-27 BD Trypticase Soy Broth with 6.5 % Sodium Chloride 3-27 BD Trypticase Soy Broth, Modified 3-41 BD Vacutainer - Anaerobic Specimen Collector 1-3 BD Vacutainer Blood Transfer device 8-5 BD Vacutainer Direct Draw Adapter 8-5 BD Vacutainer holder 8-4 BD Vacutainer holder 8-5 BD Vacutainer Luer Adapter 8-5 BD Vacutainer One Use Holder 8-4 BD Vacutainer One Use Holder 8-5 BD Vacutainer Safety-Lok Blood Collection Set 8-4 BD Vacutainer Safety-Lok Blood Collection Set (Pediatric Use) 8-4 BD Vacutainer SST II Advance 8-5 BD VZVscan - Card Test Kit 5-13 BD Dermatoslide 3-33 BD Mueller Hinton Agar with 5 % Sheep Blood 3-13 BD Mueller Hinton Agar with 5 % Sheep Blood 3-9 BD Mueller Hinton Chocolate Agar with BD IsoVitaleX 3-9 BD Mueller Hinton II Agar 3-13 BD Mueller Hinton II Agar 3-9 BD Tryptic Soy Agar 3-31 BD Universal Viral Transport Combo Kit 1-7 BD Universal Viral Transport Polyester Swabs 1-7 BD Universal Viral Transport Standard Kit 1-7 BD Universal Viral Transport Standard Polyester Swabs 1-7 BD Universal Viral Transport Vial 1-7 B Beef Heart for Infusion 2-4 Beef Heart for Infusion 2-41 Bifidobacterium Agar, Modified (Beerens) 3-2 BiGGY Agar 2-4 BiGGY Agar 3-2 Bile Esculin Agar 3-18 Bile Esculin Agar 2-4 Bile Salts No Biotin Assay Medium 2-4 Bismuth Sulfite Agar 2-5 Blood Agar Base 2-5 Bordet Gengou Agar Base 2-5 Bordet Gengou Agar with 15 % Sheep Blood 3-2 Bordetella Agar with Charcoal and 7 % Horse Blood 3-2 Bordetella Parapertussis Antiserum 6-7 Bordetella Pertussis Antigen 6-3 Bordetella Pertussis Antiserum 6-7 Bottle Phosphate Buffer 3-30 Bovine Albumin (5 %) 2-42 Bovine Albumin (Fraction V) 2-42 Brain Heart CC Agar 2-5 Brain Heart Infusion 2-41 Brain Heart Infusion 2-5 Brain Heart Infusion Agar 2-5 Brain Heart Infusion Agar 3-2 Brain Heart Infusion Agar with 10 % Sheep Blood 3-18 Brain Heart Infusion Agar with 10 % Sheep Blood (Deep Fill) 3-3 Brain Heart Infusion Agar with 5 % Horse Blood and Bacitracin 3-3 Brain Heart Infusion Broth 3-18 Brain Heart Infusion Broth 3-28 Brain Heart Infusion Broth, Modified 2-5 Brain Heart Infusion CC Agar with 10 % Sheep Blood and Gentamicin 3-18 Brain Heart Infusion with 0.1 % Agar 3-18 Brain Heart Infusion with 6,5 % Sodium Chloride 3-18 Brain Heart Infusion with Fildes Enrichment 3-18 Brain Heart Infusion with PAB and Agar 2-5 Brain Heart Infusion with PABA 2-5 BD Diagnostics Diagnostic Systems Catalog 2008/

240 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media B Brain Heart Infusion, Porcine 2-41 Brain Heart Infusion, Porcine 2-5 Brewer Anaerobic Agar 2-5 Brewer Anaerobic Agar with Lecithin and Polysorbate Brilliant Green Agar 2-5 Brilliant Green Agar, Modified 3-3 Brilliant Green Agar, Modified (Edel-Kampelmacher) 2-6 Brilliant Green Bile Agar 2-6 Brilliant Green High Selectivity Agar 3-3 Brilliant Green Lactose Bile Broth, 2 % 2-6 Brilliant Green Sulfa Agar 2-6 Brom Thymol Blue 6-20 Bromcresol Purple 6-20 Bromcresol Purple Lactose Agar 3-3 Brucella Abortus - Antigen 6-2 Brucella Agar 2-6 Brucella Agar with 5 % Horse Blood 3-3 Brucella Agar with 5 % Sheep Blood, Hemin and Vitamin K1 3-3 Brucella Broth 2-6 Brucella Positive Control - Antiserum (AMS) 6-6 Buffered Peptone Water 2-6 Buffered Peptone Water 3-28 Buffered Peptone Water with 0.1 % Tween 3-28 Buffered Sodium Chloride-Peptone Solution (ph 7.0) 3-28 Bushnell-Haas Broth 2-6 C Calcofluor White 6-14 Campylobacter Agar (Butzler) with 7 % Horse Blood 3-3 Campylobacter Agar Base 2-6 Campylobacter Agar Skirrow 3-3 Campylobacter Agar with 5 Antimicrobics and 10 % Sheep Blood (CampyBAP) 3-4 Campylobacter Selective Medium (Preston, Bloodfree) 3-4 Campylobacter Thioglycollate Medium with 5 Antimicrobics 3-18 Candida BCG Agar Base 2-6 Cary and Blair Transport Medium 2-6 Casman Agar Base 2-6 C CDC Anaerobe 5 % Sheep Blood Agar 3-4 CDC Anaerobe 5 % Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Agar with Laked Sheep Blood, Kanamycin and Vancomycin CDC Anaerobe Blood Agar Base 3-35 CDC Anaerobe with Penicillinase 3-35 Cellobiose 2-42 Cepacia Medium 3-4 Cetrimide Agar Base - BD Pseudosel Agar 2-6 Chapman Stone Medium 2-6 Charcoal Agar 2-7 Chicken Pancrease 6-21 Chocolate Agar (Blood Agar No. 2 Base) 3-4 Chocolate Agar (GC Agar with BD IsoVitaleX ) 3-4 Chocolate Agar with BD IsoVitaleX and Bacitracin 3-4 Chocolate II Agar (GC II Agar with Hemoglobin and BD IsoVitaleX ) 3-19 Choline Assay Medium 2-7 Chopped Meat Carbohydrate Broth (PR II) 3-19 CLED Agar 2-7 CLED Agar 3-4 CLED Agar (Bevis) 3-4 Clostridium Difficile Agar with 7 % Sheep Blood 3-4 Clostridium Difficile Selective Agar 3-5 Coagulase Mannitol Agar 2-7 Coagulase Plasma 6-17 Coagulase Plasma with EDTA 6-17 Columbia Agar Base 2-7 Columbia Agar with 5 % Horse Blood 3-5 Columbia Agar with 5 % Sheep Blood 3-5 Columbia Blood Agar Base 2-7 Columbia Blood Agar Base EH 2-7 Columbia Broth 2-7 Columbia CNA Agar 2-7 Columbia CNA Agar with 5 % Sheep Blood 3-5 Columbia CNA Agar with 5 % Sheep Blood // MacConkey II Agar 3-15 Columbia CNA Agar, Modified, with 5 % Sheep Blood 3-5 Catalase 6-14 Columbia CNA Agar, Modified, with Crystal Violet and 5 % Sheep Blood BD Diagnostics Diagnostic Systems Catalog 2008/2009

241 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media C Columbia CNA Plus Agar with 5 % Sheep Blood 3-5 Columbia II Agar 2-7 Columbia III Agar with 5 % Sheep Blood 3-5 Cooke Rose Bengal Agar 2-7 Cooked Meat Medium 2-7 Cooked Meat Medium 3-19 Cooked Meat Medium with Glucose, Hemin and Vitamin K Corn Meal Agar 2-7 Crystal Violet - Gentian Violet 6-20 CTA Agar 2-8 Cystine Assay Medium 2-8 Cystine Heart Agar 2-8 Cystine Tryptic Agar 2-8 Czapek Solution Agar 2-8 Czapek-Dox Broth 2-8 D D/E Neutralizing Agar 2-8 D/E Neutralizing Agar 3-35 D/E Neutralizing Agar 3-38 D/E Neutralizing Agar 3-5 D/E Neutralizing Agar - Contact Plates (65 mm) 3-34 D/E Neutralizing Agar with Penicillinase 3-35 D/E Neutralizing Broth 2-8 D/E Neutralizing Broth 3-19 D/E Neutralizing Broth 3-28 DCLS Agar 2-8 DCLS Agar 3-5 DCLS Agar, modified // Hektoen Enteric Agar 3-15 Decarboxylase Base Moeller 2-8 Decarboxylase Broth Base Moeller 2-8 Decarboxylase Medium Base 2-8 Demi-Fraser Broth Base 2-8 Dermatophyte Test Medium Agar 3-28 Dermatophyte Test Medium Base 2-9 Dermatophyte Test Medium, Modified with Chloramphenicol 3-19 Dermatophyte Test Medium, Modified with Chloramphenicol 3-6 Desoxycholate 6-14 D Desoxycholate Agar 2-9 Desoxycholate Agar 3-6 Desoxycholate Citrate Agar 2-9 Desoxycholate Lactose Agar 2-9 Dextrose Agar 2-9 Dextrose Broth 2-9 Dextrose Glucose 2-42 Dextrose Starch Agar 2-9 Dextrose Tryptone Agar 2-9 D-Galactose 2-42 Differential Reinforced Clostridial Agar (DRCA) 2-9 DMACA Indole 6-14 D-Mannitol 2-42 DNase Test Agar 2-9 DNase Test Agar 3-6 DNase Test Agar with Methyl Green 2-9 Dobell & O'Connor Iodine 6-14 Dorset Egg Medium, Modified 3-28 DRBC Agar 2-9 Drigalski Lactose Agar 3-6 Drigalski Lactose Agar with CAZ (Ceftazidime) 3-6 D-Sorbitol Sorbite 2-42 Dubos Broth Base 2-9 Dubos Broth, Enriched 3-19 Dubos Medium Albumin 2-33 Dubos Oleic Agar Base 2-9 Dubos Oleic Albumin Complex 2-33 Dulcitol Galactitol 2-42 D-Xylose 2-42 E E. Coli H Antiserum H7 6-7 E. Coli O Antiserum O EC Medium 2-10 EC Medium with MUG 2-10 EC Medium, Modified 2-10 EE Broth 2-10 EE Broth 3-28 BD Diagnostics Diagnostic Systems Catalog 2008/

242 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media E EE Broth Mossel 2-10 Egg Yolk Enrichment (50 %) 2-34 Egg Yolk Enrichment (Gamma Irradiated) 2-34 Egg Yolk Tellurite Enrichment 2-34 Egg Yolk Tellurite Enrichment (Gamma Irradiated) 2-34 Elliker Broth 2-10 EMB Agar, Modified (Holt-Harris and Teague) 3-6 Endo Agar 2-10 Endo Agar 3-6 Enterobacteriaceae Enrichment Broth (EE Broth Mossel) 3-28 Enterococcus Screen Agar 3-6 Eosin Methylene Blue Agar (Levine) Without Lactose 2-10 Eosin Methylene Blue Agar, Modified (Holt-Harris & Teague) 2-10 Esculin 2-42 Esculin Iron Agar 2-10 Eugon Agar 2-10 Eugon Broth 2-11 European Agar 2-2 EVA Broth 2-11 F FA Bordetella Parapertussis 6-18 FA Bordetella Pertussis 6-18 FA Buffer (Dried) 2-11 FA Buffer (Dried) 6-18 Febrile Antigen Kit 6-2 Febrile Antigen Negative Control (optional) 6-2 Ferric Chloride 6-15 Fildes Enrichment 2-34 Flagella 6-15 Fletcher Medium Base 2-11 Fluid A 3-29 Fluid A 3-39 Fluid A (Sterile Pack Bottle) 3-39 Fluid D 3-39 Fluid D (Sterile Pack Bottle) 3-39 Folic Acid Assay Medium 2-11 Folic Acid Casei Medium 2-11 F Folic AOAC Medium 2-11 Francisella Tularensis - Antigen 6-2 Francisella Tularensis - Antiserum 6-6 Fraser Broth Base 2-11 Fraser Broth Supplement 2-34 FTA Hemagglutination Buffer 2-11 FTA Hemagglutination Buffer 6-18 G Gardnerella Selective Agar with 5 % Human Blood 3-6 GC II Agar with BD IsoVitaleX Enrichment 3-14 GC Medium Base 2-11 Gelatin 2-2 Gelatin 2-41 Giolitti-Cantoni Broth Base 2-11 Glycerol 2-42 GN Broth 2-11 GN Broth 3-19 GN Broth - Hajna 2-11 Gottsacker TB Medium 3-20 Gottsacker TB Medium 9-6 Gram Basic Fuchsin 6-19 Gram Crystal Violet 6-19 Gram Decolorizer 6-19 Gram Iodine (Stabilized) 6-19 Gram Iodine (Unstabilized) 6-19 Gram Quality Control Slide 6-19 Gram Safranin 6-19 Gram Stain Kit (Stabilized) 6-19 Gram Stain Kit (Unstabilized) 6-19 Group A Selective Strep Agar with 5 % Sheep Blood (BD ssa ) Group A Selective Strep Agar with 5 % Sheep Blood (BD ssa ) // BD Trypticase Soy Agar with 5 % Sheep Blood (TSA II) Group B Streptococcus Differential Agar 3-7 GVPC Medium 3-7 H Haemophilus Identification Plate (Hemo ID Plate) 3-13 Haemophilus Influenzae Antiserum Poly BD Diagnostics Diagnostic Systems Catalog 2008/2009

243 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media H Haemophilus Influenzae Antiserum Type a 6-7 Haemophilus Influenzae Antiserum Type b 6-7 Haemophilus Influenzae Antiserum Type c 6-7 Haemophilus Influenzae Antiserum Type d 6-7 Haemophilus Influenzae Antiserum Type e 6-7 Haemophilus Influenzae Antiserum Type f 6-7 Haemophilus Test Medium Agar 3-14 Haemophilus Test Medium Agar 3-7 HC Agar Base 2-11 Heart Infusion Agar 2-12 Heart Infusion Agar with 5 % Sheep Blood 3-7 Heart Infusion Broth 2-12 Hektoen Enteric Agar 2-12 Hektoen Enteric Agar 3-7 Helicobacter Agar 3-7 Hemoglobin Solution (2 %) 2-42 Hemoglobin, Bovine (Freeze-Dried) 2-12 Herrold's Egg Yolk Agar with Mycobactin J and ANV 3-20 Herrold's Egg Yolk Agar with Mycobactin J and ANV 9-6 Herrold's Egg Yolk Agar without Mycobactin J with ANV 3-20 Herrold's Egg Yolk Agar without Mycobactin J with ANV 9-6 I Inactivating Solution 3-29 India Ink 6-15 Indole 6-15 Indole Nitrite Medium (BD Trypticase Nitrate Broth) 2-12 Inositol 2-43 Inositol Assay Medium 2-12 Iodine Solution 2-34 Iso Res Agar 3-7 Isopropylmyristate 3-39 ISP Medium ISP Medium ISP Medium J Jembec - Modified Thayer-Martin (MTM II) Agar 1-3 Jordan's Tartrate Agar 3-20 K KF Streptococcus Agar 2-12 KF Streptococcus Broth 2-12 Kimmig Fungal Agar 3-7 Kirchner Medium 3-29 Kligler Iron Agar 3-20 Kligler Iron Agar 2-12 L Lactobacilli Agar AOAC 2-12 Lactobacilli Broth AOAC 2-12 Lactobacilli MRS Agar 2-12 Lactobacilli MRS Broth 2-13 Lactophenol Cotton Blue 6-15 Lactose Broth 2-13 Lactose Broth 3-29 Lactose Broth (Double Strength) 3-29 Lactose Broth with Durham Tube (Double Strength) 3-20 Lactose Broth with Stopper 3-29 Lactose Monohydrate 2-13 Lactose Peptone Broth 2-13 L-Arabinose 2-43 Lauryl Sulfate Broth 2-13 Lauryl Sulfate Broth with Durham Tube (Double Strength) 3-20 Lauryl Sulfate Broth with MUG 2-13 Lauryl Tryptose Broth 2-13 Lauryl Tryptose Broth with MUG 2-13 LB Agar (Lennox) 2-39 LB Agar (Lennox) 3-7 LB Agar (Miller) 2-39 LB Broth (Lennox) 2-39 LB Broth (Miller) 2-39 LB Broth (Miller) 3-29 LBS Agar 2-13 LBS Agar 3-7 LBS Broth 2-13 Legionella Agar (NEN) with Antibiotics 3-8 Legionella Agar (NEN) without Antibiotics with Pimaricin 3-8 Legionella Agar Enrichment 2-34 BD Diagnostics Diagnostic Systems Catalog 2008/

244 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media L Leptospira Enrichment (Lyophilized) 2-35 Leptospira Enrichment EMJH 2-35 Leptospira Medium Base EMJH 2-13 Letheen Agar 2-13 Letheen Agar, Modified 2-13 Letheen Broth 2-13 Letheen Broth, Modified 2-14 Letheen Broth, Modified 3-29 Levine EMB Agar 2-14 Levinthal Agar Base - double sterile 3-29 Levinthal Stock Broth 3-29 LIM Broth (Todd Hewitt with CNA) 3-20 Lipase Reagent 2-35 Listeria Agar 3-8 Listeria Enrichment Broth 2-14 Listeria Enrichment Broth 2-35 Listeria Enrichment Broth, Modified 2-14 Listeria Enrichment Broth, Modified 2-35 Listeria O Antigen Type 1 (Slide) 6-3 Listeria O Antigen Type 4 (Slide) 6-3 Listeria O Antiserum Poly 6-8 Listeria O Antiserum Type Listeria O Antiserum Type Listeria Selective Supplement 2-35 Litmus Milk 2-14 Liver (Desiccated Powder) 2-14 Liver Infusion Agar 2-14 Liver Infusion Broth 2-14 Liver Veal Agar 2-14 Loeffler Blood Serum 2-14 Loeffler Medium 3-20 Loewenstein Medium Base 2-14 Loewenstein Medium Base 9-5 Loewenstein-Jensen Medium 3-20 Loewenstein-Jensen Medium 9-6 Loewenstein-Jensen Medium (BD Mycoflask ) 3-21 Loewenstein-Jensen Medium (BD Mycoflask ) 9-6 Loewenstein-Jensen Medium with 5 % Sodium Chloride 3-21 L Loewenstein-Jensen Medium with 5 % Sodium Chloride 9-6 Loewenstein-Jensen Medium with Glycerol and PACT 3-21 Loewenstein-Jensen Medium with Glycerol and PACT 9-6 LPM Agar Base 2-14 Luria Agar Base (Miller) 2-39 Luria Broth Base (Miller) 2-39 Lysine Decarboxylase Broth 2-14 Lysine Iron Agar 2-14 Lysine Iron Agar 3-21 M M Broth 2-15 m Endo Agar LES 2-15 m Endo Broth MF 2-15 m Enterococcus Agar 2-15 m FC Agar 2-15 m FC Broth Base 2-15 m HPC Agar 2-15 m Plate Count Broth 2-15 m Staphylococcus Broth 2-15 m TEC Agar 2-15 m TGE Broth 2-15 M. butyricum (Desiccated) 6-13 M. Tuberculosis H37 Ra (Desiccated) 6-13 M17 Agar 2-15 M17 Broth 2-15 M9 Minimal Salts (5 ) 2-40 MacConkey Agar 2-16 MacConkey Agar // Columbia CNA Agar with 5 % Sheep Blood 3-15 MacConkey Agar Base 2-16 MacConkey Agar without Crystal Violet 2-16 MacConkey Agar without Crystal Violet 3-8 MacConkey Agar without Crystal Violet or Salt 2-16 MacConkey Agar without Salt 3-8 MacConkey Agar without Salt 2-16 MacConkey Broth 3-29 MacConkey Broth 2-16 MacConkey Broth with Durham Tube BD Diagnostics Diagnostic Systems Catalog 2008/2009

245 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media M MacConkey II Agar 2-16 MacConkey II Agar 3-8 MacConkey II Agar with MUG 3-8 MacConkey II Agar with Sorbitol 3-21 MacConkey II Agar with Sorbitol 3-8 MacConkey Sorbitol Agar 2-16 Malonate Broth 2-16 Malonate Broth, Ewing Modified 2-16 Malonate Broth, Ewing Modified 3-21 Malonate Broth, Modified 2-16 Malt Agar 2-16 Malt Extract Agar 2-16 Malt Extract Broth 2-16 Maltose 2-43 Mannitol Salt Agar 2-17 Mannitol Salt Agar 3-8 Mannitol Salt Agar with Oxacillin 3-8 Marine Agar Marine Broth Martin-Lewis Agar, Modified 3-8 Maximum Recovery Diluent 2-17 McClung Toabe Agar Base 2-17 McFarland Turbidity 7-23 Melibiose 2-43 Methylene Blue Loeffler 6-16 M-Green Yeast and Mold Broth 2-17 MI Agar 2-17 Micro Assay Culture Agar 2-17 Micro Inoculum Broth 2-17 Microbial Content Test Agar 2-17 Middlebrook 7H10 // 7H11 Selective Agar 3-15 Middlebrook 7H10 // 7H11 Selective Agar 9-6 Middlebrook 7H10 Agar 2-17 Middlebrook 7H10 Agar 9-5 Middlebrook 7H11 Agar Base 2-17 Middlebrook 7H11 Agar Base 9-5 Middlebrook 7H9 Broth 2-18 Middlebrook 7H9 Broth 9-5 M Middlebrook 7H9 Broth with Glycerol 9-6 Middlebrook 7H9 Broth with Glycerol 3-21 Middlebrook ADC Enrichment 2-35 Middlebrook ADC Enrichment 9-5 Middlebrook and Cohn 7H10 Agar 3-21 Middlebrook and Cohn 7H10 Agar 3-9 Middlebrook and Cohn 7H10 Agar 9-7 Middlebrook OADC Enrichment 2-35 Middlebrook OADC Enrichment 9-5 MIL Medium 2-18 Milk Agar 2-18 Minerals Modified Glutamate Broth 2-18 Minimal Agar Davis 2-18 Minimal Broth Davis without Dextrose 2-18 MIO Medium 2-18 Mitis Salivarius Agar 2-18 Moeller Decarboxylase Broth Base with Lysine 3-21 Moeller Decarboxylase Broth with Ornithine 3-21 Motility GI Medium 2-18 Motility Indole Ornithine Medium (MIO Medium) 3-22 Motility Test Medium 2-18 Motility Test Medium 3-22 M-PA-C Agar 2-18 MR-VP Broth 2-18 MR-VP Medium 2-18 Mucin Bacteriological 6-13 Mueller Hinton Agar with 5 % Sheep Blood 3-14 Mueller Hinton Broth 2-19 Mueller Hinton Broth 3-22 Mueller Hinton II Agar 3-14 Mueller Hinton II Agar 2-19 Mueller Hinton II Broth 2-19 Mueller Hinton II Broth 3-22 Mueller Hinton Medium 2-19 Muller Kauffmann Tetrathionate Broth Base 2-19 Mycobacteria 7H11 Agar 2-19 Mycological Agar 2-19 Mycoplasma Agar Base (PPLO Agar) 2-19 BD Diagnostics Diagnostic Systems Catalog 2008/

246 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media M Mycoplasma Broth Base (Frey) 2-20 Mycoplasma Broth Base (PPLO Broth Base) 2-20 Mycoplasma Enrichment without Penicillin 2-36 Mycoplasma Supplement 2-36 Mycoplate MS Agar 3-9 MYP Agar 2-20 MYP Agar 3-9 N Neisseria Meningitidis Antiserum Group A 6-8 Neisseria Meningitidis Antiserum Group B 6-8 Neisseria Meningitidis Antiserum Group C 6-8 Neisseria Meningitidis Antiserum Group D 6-8 Neisseria Meningitidis Antiserum Group W Neisseria Meningitidis Antiserum Group X 6-8 Neisseria Meningitidis Antiserum Group Y 6-8 Neisseria Meningitidis Antiserum Group Z 6-8 Neisseria Meningitidis Antiserum Group Z' 6-8 Neisseria Meningitidis Antiserum Poly 6-8 Neisseria Meningitidis Antiserum Poly Neomycin Blood Agar 3-9 Neutralizing Buffer 2-20 Niacin Assay Medium 2-20 NIH Thioglycollate Broth (USP Alternative Thioglycollate Medium) 2-20 Ninhydrin 6-16 Nitrate Broth 2-20 Nitrate Reagent A 6-16 Nitrate Reagent B 6-16 Nitrocefin Powder 7-9 Novobiocin Antimicrobic Supplement 2-36 Nutrient Agar 2-20 Nutrient Agar 3-22 Nutrient Agar 3-9 Nutrient Agar - Special Contact Plates 3-34 Nutrient Agar (1.5 %) 2-20 Nutrient Agar (ph 6.0) 2-20 Nutrient Agar with MUG 2-20 Nutrient Broth 3-22 N Nutrient Broth 3-30 Nutrient Broth 2-21 Nutrient Gelatin 2-21 NZCYM Broth 2-40 NZYM Broth 2-40 O Oatmeal Agar 2-21 OF Basal Medium 2-21 OF Basal Medium 3-22 OF Basal Medium with Dextrose 3-22 OFPBL Agar 3-9 OGYE Agar Base 2-21 Orange Serum Agar 2-21 Orange Serum Broth Concentrate (10 x) 3-22 Oxacillin Screen Agar 3-10 Oxford Antimicrobic Supplement, Modified 2-36 Oxford Medium Base 2-21 Oxgall 2-43 Oxidase 6-16 P PALCAM Antimicrobic Supplement 2-36 PALCAM Listeria Agar 3-10 PALCAM Listeria Agar 3-14 PALCAM Medium Base 2-21 Pantothenate Assay Medium 2-21 Pantothenate Medium AOAC 2-21 Penase 6-21 Penase Concentrate 6-21 Penicillinase 6-21 Penicillinase Concentrate 6-21 Peptone Iron Agar 2-21 Peptone Water 2-21 Peptone Water (0.1 %) 3-30 Peptone Water (0.1 %, DEP) 3-39 Peptone Water with Glucose and Durham Tube 3-30 Petragnani Medium 3-22 Phenol Red BD Diagnostics Diagnostic Systems Catalog 2008/2009

247 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media P Phenol Red Agar Base 2-21 Phenol Red Broth Base 2-21 Phenol Red Broth with Dextrose and Durham Tube 3-23 Phenol Red Broth with Xylose and Durham Tube 3-23 Phenol Red Dextrose Broth 2-22 Phenol Red Lactose Broth 2-22 Phenol Red Mannitol Agar 2-22 Phenol Red Mannitol Broth 2-22 Phenol Red Sucrose Broth 2-22 Phenylalanine Agar 2-22 Phenylalanine Agar 3-23 Phenylethyl Alcohol Agar 2-22 Phosphate Buffered Saline (PBS) 3-30 Phosphate Buffered Saline (ph 7.0) 3-30 Plate Count Agar 2-22 Plate Count Agar 3-10 Polysorbate Potato Dextrose Agar 2-22 Potato Dextrose Broth 2-22 Potato Glucose Agar 3-10 Potato Infusion Agar 2-22 PPLO Agar (Mycoplasma Agar) 2-22 PPLO Broth (Mycoplasma Broth) 2-22 Presence-Absence Broth 2-23 Proteose No. 3 Agar 2-23 Proteus OX19 - Antigen 6-2 Proteus OX2 - Antigen 6-2 Proteus OXK - Antigen 6-2 Proteus Polyvalent - Antiserum (OXK, OX2, OX19) 6-6 Pseudomonas Agar F 2-23 Pseudomonas Agar P 2-23 Pseudomonas Agar P 3-10 Pseudomonas CFC Agar 3-10 Pseudomonas Isolation Agar 2-23 Pseudomonas Isolation Agar 3-10 Purple Agar Base 2-23 Purple Broth Base 2-23 PYR 6-17 P Pyridoxine Y Medium 2-23 Q QC Antigen Salmonella O - Group A 6-3 QC Antigen Salmonella O - Group B 6-3 QC Antigen Salmonella O - Group C1 6-3 QC Antigen Salmonella O - Group C2 6-3 QC Antigen Salmonella O - Group D 6-4 QC Antigen Salmonella O - Group E1 6-4 QC Antigen Salmonella O - Group E2 6-4 QC Antigen Salmonella O - Group E4 6-4 QC Antigen Salmonella O - Group F 6-4 QC Antigen Salmonella O - Group G1 6-4 QC Antigen Salmonella O - Group H 6-4 QC Antigen Salmonella O - Group I 6-4 QC Antigen Salmonella O - Group Vi 6-5 QC Antigen Shigella - Group A 6-5 QC Antigen Shigella - Group A1 6-5 QC Antigen Shigella - Group B 6-5 QC Antigen Shigella - Group C 6-5 QC Antigen Shigella - Group C1 6-5 QC Antigen Shigella - Group C2 6-5 QC Antigen Shigella - Group D 6-5 R R2A Agar 2-23 R2A Agar 3-10 Raffinose 2-43 Raka-Ray No. 3 Medium 2-23 Rapid Fermentation Medium 3-23 Rapid Urea Broth 3-23 Rappaport-Vassiliadis Broth (RV Broth) 3-23 Rappaport-Vassiliadis Medium, Modified (Semisolid) 2-23 Rappaport-Vassiliadis Medium, Modified (Semisolid) 3-30 Rappaport-Vassiliadis R10 Broth 2-23 Rappaport-Vassiliadis Soy Peptone Broth 3-30 Regan-Lowe Charcoal Agar Base 2-23 Reinforced Clostridial Medium (RCM) 2-24 Rhamnose 2-43 BD Diagnostics Diagnostic Systems Catalog 2008/

248 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media R Riboflavin Assay Medium 2-24 Rice Extract Agar 2-24 Rice Extract Agar 3-11 Rinsing Fluid D 3-39 Rogosa SL Agar 2-24 Rogosa SL Broth 2-24 Rose Bengal Agar Base 2-24 Rose Bengal Agar with Chloramphenicol - Contact Plates (65 mm) 3-34 Rose Bengal Agar with Penicillinase 3-35 Rose Bengal Antimicrobic Supplement 2-36 Rosolic Acid 2-36 S Sabouraud Agar with Chloramphenicol 3-11 Sabouraud Agar with Chloramphenicol and Cycloheximid 3-11 Sabouraud Agar with Gentamycin and Chloramphenicol 3-11 Sabouraud Agar with Penicillin and Streptomycin 3-11 Sabouraud Agar, Modified 2-24 Sabouraud Brain Heart Infusion Agar Base 2-24 Sabouraud Brain Heart Infusion Agar with Chloramphenicol and Gentamicin 3-23 Sabouraud Dextrose Agar 3-35 Sabouraud Dextrose Agar 2-24 Sabouraud Dextrose Agar 3-11 Sabouraud Dextrose Agar 3-23 Sabouraud Dextrose Agar (ph 5.6) 3-23 Sabouraud Dextrose Agar with 2 % Dextrose - Contact Plates 3-34 Sabouraud Dextrose Agar with Chloramphenicol 3-23 Sabouraud Dextrose Agar with Chloramphenicol 3-35 Sabouraud Dextrose Agar with Lecithin and Polysorbate Sabouraud Dextrose Agar with Lecithin, Polysorbate 80 and Chloramphenicol 3-38 Sabouraud Dextrose Broth 2-24 Sabouraud Dextrose CC Agar 3-24 Sabouraud Glucose Agar 3-30 Sabouraud Glucose Agar (Gamma-Irradiated) 3-35 Sabouraud Glucose Agar with Chloramphenicol 3-37 Sabouraud Glucose Agar with Chloramphenicol - Contact Plates 3-34 S Sabouraud Glucose Agar with Gentamycin and Chloramphenicol // BD CHROMagar Candida 3-15 Sabouraud Liquid Broth, Modified (Antibiotic Assay Medium 13) 3-24 Sabouraud Maltose Agar 2-24 Sabouraud Maltose Broth 2-24 Sabouraud Medium, Fluid 2-24 Saccharose 2-43 Salicin 2-43 Saline Solution (0.9 %) 3-30 Saline, Normal 3-24 Salmonella Antiserum Vi 6-12 Salmonella Flagellar - Antigen a 6-2 Salmonella Flagellar - Antigen b 6-2 Salmonella Flagellar - Antigen d 6-2 Salmonella Flagellar Poly - Antiserum (a, b, d) 6-6 Salmonella H - Antiserum 1 Complex 6-10 Salmonella H - Antiserum a 6-8 Salmonella H - Antiserum b 6-8 Salmonella H - Antiserum c 6-8 Salmonella H - Antiserum d 6-8 Salmonella H - Antiserum eh 6-8 Salmonella H - Antiserum EN Complex 6-9 Salmonella H - Antiserum f 6-8 Salmonella H - Antiserum G Complex 6-9 Salmonella H - Antiserum h 6-8 Salmonella H - Antiserum i 6-8 Salmonella H - Antiserum k 6-9 Salmonella H - Antiserum L Complex 6-9 Salmonella H - Antiserum m 6-9 Salmonella H - Antiserum p 6-9 Salmonella H - Antiserum Poly A (a,b,c,d,i,z10,z29) 6-9 Salmonella H - Antiserum Poly a-z 6-9 Salmonella H - Antiserum Poly B (eh,en,enx,enz15, and G Complex) 6-10 Salmonella H - Antiserum Poly C (k,l,r,y,z,z4) 6-10 Salmonella H - Antiserum Poly D (z35,z36,z37,z38,z39,z41,z Salmonella H - Antiserum Poly E (1 Complex, z6) 6-10 Salmonella H - Antiserum r 6-9 Salmonella H - Antiserum s BD Diagnostics Diagnostic Systems Catalog 2008/2009

249 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media S Salmonella H - Antiserum Single Factor Salmonella H - Antiserum Single Factor Salmonella H - Antiserum Single Factor Salmonella H - Antiserum Single Factor Salmonella H - Antiserum Spicer-Edwards Salmonella H - Antiserum Spicer-Edwards Salmonella H - Antiserum Spicer-Edwards Salmonella H - Antiserum Spicer-Edwards Salmonella H - Antiserum t 6-9 Salmonella H - Antiserum w 6-9 Salmonella H - Antiserum x 6-9 Salmonella H - Antiserum y 6-9 Salmonella H - Antiserum z 6-9 Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum z Salmonella H - Antiserum Z4 Complex 6-9 Salmonella H - Antiserum z6 6-9 Salmonella O - Antigen Group A 6-2 Salmonella O - Antigen Group B 6-2 Salmonella O - Antigen Group D 6-2 Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor S Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Factor Salmonella O - Antiserum Group A Factors 1, 2, Salmonella O - Antiserum Group B Factors 1, 4, 12, Salmonella O - Antiserum Group B Factors 1, 4, 5, Salmonella O - Antiserum Group C1 Factors 6, Salmonella O - Antiserum Group C2 Factors 6, Salmonella O - Antiserum Group C3 Factors (8), Salmonella O - Antiserum Group D1 Factors 1, 9, Salmonella O - Antiserum Group D2 Factors (9), Salmonella O - Antiserum Group E Factors 1, 3, 10, 15, 19, Salmonella O - Antiserum Group E1 Factors 3, Salmonella O - Antiserum Group E2 Factors 3, Salmonella O - Antiserum Group E3 Factors (3), (15), Salmonella O - Antiserum Group E4 Factors 1, 3, Salmonella O - Antiserum Group F Factor Salmonella O - Antiserum Group G Factors 13, 22, 23, (36), (37) 6-12 Salmonella O - Antiserum Group G1 Factors 13, 22, (36) 6-12 Salmonella O - Antiserum Group G2 Factors 1, 13, 23, (37) 6-12 Salmonella O - Antiserum Group H Factors 1, 6, 14, 24, Salmonella O - Antiserum Group I Factor Salmonella O - Antiserum Group J Factor Salmonella O - Antiserum Group K Factor Salmonella O - Antiserum Group L Factor Salmonella O - Antiserum Group M Factor Salmonella O - Antiserum Group N Factor Salmonella O - Antiserum Group O Factor Salmonella O - Antiserum Poly A (A, B, D, E1, E2, E3, E4, & L) 6-12 Salmonella O - Antiserum Poly A-I & Vi 6-12 Salmonella O - Antiserum Poly B (C1, C2, F, G, & H) 6-12 Salmonella O - Antiserum Poly C (I, J, K, M, N, & O) 6-12 Salmonella O - Antiserum Poly D (P, Q, R, S, T, & U) 6-12 Salmonella O - Antiserum Poly E (V, W, X, Y, & Z) 6-12 Salmonella O - Antiserum Poly F (Groups 51-55) 6-12 Salmonella O - Antiserum Poly G (Groups 56-61) 6-12 BD Diagnostics Diagnostic Systems Catalog 2008/

250 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media S Salmonella O - Antiserum, Factors 4, Salmonella O - Antiserum, Group A 6-6 Salmonella O - Antiserum, Group D 6-6 Salmonella O - Antiserum, Group H 6-6 Salmonella Shigella Agar 3-11 Salmonella Shigella Agar 2-25 Salmonella Somatic Poly - Antiserum (A, B, D) 6-6 SBG Sulfa Enrichment 2-36 Schaedler Agar 2-25 Schaedler Agar // Schaedler Agar KV (Vitamin K1, Kanamycin, Vancomycin) 3-16 Schaedler Agar with Vitamin K1 and 5 % Sheep Blood 3-11 Schaedler Broth 2-25 Schaedler Broth with Vitamin K Schaedler CNA Agar with 5 % Sheep Blood 3-11 Schaedler K-V Agar with 5 % Sheep Blood 3-11 Selenite Broth 2-25 Selenite Cystine Broth 2-25 Selenite Cystine Broth 3-24 Selenite-F Broth 3-24 Serum Tellurite Agar 3-11 Serum Tellurite Agar 3-24 Seven H11 Agar (Deep Fill) 9-7 Seven H11 Agar (Deep Fill) 3-11 Seven H11 Agar (Middlebrook 7H11 Agar) 3-24 Seven H11 Agar (Middlebrook 7H11 Agar) 9-7 Seven H11 Agar with Aspartic Acid and Sodium Pyruvate 3-24 Seven H11 Agar with Aspartic Acid and Sodium Pyruvate 9-7 SF Broth 3-24 SF Medium 2-25 SFP Agar Base 2-25 Shigella Antiserum Poly Group A 6-13 Shigella Antiserum Poly Group A 6-6 Shigella Antiserum Poly Group A Shigella Antiserum Poly Group B 6-13 Shigella Antiserum Poly Group C 6-13 Shigella Antiserum Poly Group C Shigella Antiserum Poly Group C S Shigella Antiserum Poly Group D 6-13 SIM Medium 2-25 SIM Medium 3-25 Simmons Citrate Agar 2-25 Simmons Citrate Agar 3-25 Skim Milk 2-25 SOB Medium 2-40 Sodium Desoxycholate 2-43 Soluble Starch 2-25 Special Yeast and Mold Medium 2-25 Spirit Blue Agar 2-26 SPS Agar 2-26 Standard Methods Agar 3-25 Standard Methods Agar 2-26 Standard Methods Agar with Lecithin and Polysorbate Staphylococcus Medium Starch Agar 2-26 Sterile disposable Inoculating Loops - 1 μl (Light Green) 1-4 Sterile disposable Inoculating Loops - 10 μl (Light Blue) 1-4 Sterile disposable Inoculating Needle (Violet) 1-4 Sterile Water 3-25 Stock Culture Agar 2-26 Stonebrink TB Medium with PACT 3-25 Stonebrink TB Medium with PACT 9-7 Sugar Free Agar 2-26 Sulfite Agar 2-26 Supplement B 2-37 Supplement C 2-37 Supplement VX 2-37 Synthetic Broth AOAC 2-26 T TAT Broth Base 2-26 TB Auramine M 6-24 TB Auramine M 9-4 TB Auramine-Rhodamine T 6-24 TB Auramine-Rhodamine T 9-4 TB Brilliant Green K BD Diagnostics Diagnostic Systems Catalog 2008/2009

251 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media T TB Brilliant Green K 9-3 TB Carbolfuchsin KF 6-23 TB Carbolfuchsin KF 9-3 TB Carbolfuchsin ZN 6-22 TB Carbolfuchsin ZN 9-3 TB Decolorizer 6-23 TB Decolorizer 9-3 TB Decolorizer 6-22 TB Decolorizer TM 6-24 TB Decolorizer TM 9-4 TB Fluorescent Stain Kit M 6-24 TB Fluorescent Stain Kit M 9-4 TB Fluorescent Stain Kit T 6-24 TB Fluorescent Stain Kit T 9-4 TB Methylene Blue 6-22 TB Methylene Blue 9-3 TB Potassium Permanganate 6-24 TB Potassium Permanganate 9-4 TB Quick Stain - Carbolfuchsin 6-22 TB Quick Stain - Carbolfuchsin 9-2 TB Quick Stain - Kit 6-22 TB Quick Stain - Kit 9-2 TB Quick Stain - Methylene Blue 6-22 TB Quick Stain - Methylene Blue 9-2 TB Stain Kit K 6-23 TB Stain Kit K 9-3 TB Stain Kit ZN 6-22 TB Stain Kit ZN 9-3 TCBS Agar 2-26 TCBS Agar 3-11 Tellurite Agar (Hoyle) with 7 % Lysed Horse Blood 3-12 Tellurite Glycine Agar 2-26 Tellurite Solution (1 %) 2-37 Tellurite Solution (1 %) 2-43 Terrific Broth 2-40 Tetrathionate Broth 3-30 Tetrathionate Broth Base 2-26 Tetrathionate Broth Base 3-25 T Tetrathionate Broth Base 3-31 Tetrathionate Broth Base - Hajna (TT Broth Base, Hajna) 2-27 The Flexible Solution for Biological Validation, Epidemiology & Nosocomial Infection Control 11-2 Thermoacidurans Agar 2-27 Thiamine Assay Medium 2-27 Thiamine Assay Medium LV 2-27 Thioglycollate Medium without Dextrose 2-27 Thioglycollate Medium without Dextrose or Indicator 2-27 Thioglycollate Medium without Indicator 2-27 Thioglycollate Medium without Indicator - 135C 2-27 Thioglycollate Medium without Indicator - 135C 3-25 Thioglycollate Medium, Brewer Modified 2-27 Thioglycollate Medium, Enriched with Calcium Carbonate 3-25 Thioglycollate Medium, Enriched with Vitamin K1 and Hemin 3-25 Thioglycollate Medium, Enriched with Vitamin K1 and Hemin 3-26 Thioglycollate Medium, Fluid 2-27 Thioglycollate Medium, Fluid 3-26 Thioglycollate Medium, Fluid 3-31 Thioglycollate Medium, Fluid 3-39 Thioglycollate Medium, Fluid (Double Strength) 3-39 Thioglycollate Medium, Fluid (FTM Special) 3-40 Thioglycollate Medium, Fluid (FTM Special, ETO sterile) 3-40 Thioglycollate Medium, Fluid (FTM Special, Sterile Pack Bottle) 3-40 Thioglycollate Medium, Fluid with 1 % Tween 3-39 Thioglycollate Medium, Fluid with Beef Extract 2-27 Thioglycollate Medium, Fluid without Dextrose or Eh Indicator 2-27 Thioglycollate Medium, Fluid, Modified 3-39 Thiol Broth 2-27 Tinsdale Agar Base 2-28 Tinsdale Enrichment 2-37 Todd Hewitt Broth 2-28 Todd Hewitt Broth 3-26 Todd Hewitt Broth 3-31 Todd Hewitt Broth with Gentamicin and Nalidixic Acid 3-26 Tomato Juice Agar 2-28 Tomato Juice Agar (Special) 2-28 Tomato Juice Broth 2-28 BD Diagnostics Diagnostic Systems Catalog 2008/

252 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media T Transport Medium (Stuart, Toshach and Patsula) 2-28 Trehalose 2-43 Trichophyton Agar Trichophyton Agar Trichophyton Agar Trichophyton Agar Trichophyton Agar Trichophyton Agar Triple Sugar Iron Agar 2-28 Triple Sugar Iron Agar 3-12 Tryptic Soy Agar 2-28 Tryptic Soy Blood Agar Base EH 2-29 Tryptic Soy Blood Agar Base No Tryptic Soy Broth 2-29 Tryptic Soy Broth 3-31 Tryptic Soy Broth (Sterile ETO) 3-31 Tryptic Soy Broth without Dextrose 2-29 Tryptone Glucose Extract Agar 2-29 Tryptone Water 2-29 Tryptose Agar 2-29 Tryptose Blood Agar Base 2-30 Tryptose Broth 2-30 Tryptose Phosphate Broth 2-30 TSI Agar 3-27 TSN Agar 2-30 TTC Solution (1 %, Sterile) 2-37 TTC Solution (1 %, Sterile) 6-20 Tween 80 - Polysorbate Tween 80 (20 %) 3-31 U Universal Beer Agar 2-30 Universal Preenrichment Broth 2-30 Universal Preenrichment Broth 2-37 Urea Agar 3-27 Urea Agar Base 2-30 Urea Agar Base Concentrate (10 ) 3-27 Urea Broth 2-30 U Urease Broth Concentrate (10 ) 3-27 Urease Test Broth 3-27 UVM Modified Listeria Enrichment Broth 2-30 V Vancomycin Screen Agar 3-12 V-C-A Inhibitor 2-38 VCAT Inhibitor 2-38 V-C-N Inhibitor 2-38 V-C-N-T Inhibitor 2-38 VDRL Cardiolpin Antigen 5-12 VDRL Test Control Serum Set 5-12 Veal Infusion Agar 2-30 Veal Infusion Broth 2-30 Vibrio Cholerae Antiserum Inaba 6-13 Vibrio Cholerae Antiserum Ogawa 6-13 Vibrio Cholerae Antiserum Poly 6-13 Violet Red Bile Agar 2-30 Violet Red Bile Agar with MUG 2-30 Violet Red Bile Glucose Agar (VRBG Agar) - Contact Plates (65 mm) 3-34 Violet Red Bile Glucose Agar (VRBG Agar) 2-30 Violet Red Bile Glucose Agar (VRBG Agar) 3-12 Vitamin B12 Assay Medium 2-31 Vitamin K1-Hemin Solution 2-38 VJ Agar 2-31 Voges-Proskauer A 6-17 Voges-Proskauer B 6-17 W Water (Sterile, Double Wrapped) 3-31 Wilkins Chalgren Agar 2-31 Wilkins Chalgren Agar 3-12 Wilkins Chalgren Agar with 5 % Horse Blood 3-12 Wilkins Chalgren Agar with Amikacin and 7 % Sheep Blood 3-12 WL Differential Medium 2-31 WL Nutrient Broth 2-31 WL Nutrient Medium 2-31 Wort Agar BD Diagnostics Diagnostic Systems Catalog 2008/2009

253 Alphabetical Index Name Page Name Page DCM = Dehydrated Culture Media // PPM = Prepared Plated Media // PTM = Prepared Tubed Media // PBM = Prepared Bottled Media X XL Agar Base 2-31 XLD Agar 2-31 XLD Agar 3-13 XLD Agar // Brilliant Green Agar, Modified 3-16 XLT4 Agar Base 2-31 XLT4 Supplement 2-38 Y Yeast Carbon Base 2-31 Yeast Extract Glucose Chloramphenicol Agar 2-31 Yeast Morphology Agar 2-32 Yeast Nitrogen Base 2-32 Yeast Nitrogen Base without Amino Acids 2-32 Yeast Nitrogen Base without Amino Acids and Ammonium Sulfate 2-32 Yersinia Antimicrobic Supplement CN 2-38 Yersinia Selective Agar (CIN Agar) 3-13 Yersinia Selective Agar Base (CIN Agar Base) 2-32 YM Agar 2-32 YM Broth 2-32 YPD Agar 2-40 YPD Broth 2-40 BD Diagnostics Diagnostic Systems Catalog 2008/

254 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/2009

255 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/

256 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/2009

257 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/

258 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/2009

259 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/

260 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/2009

261 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/

262 Numerical Index Page Page Page Page Page Page BD Diagnostics Diagnostic Systems Catalog 2008/2009

263 Numerical Index Page Page Page Page Page Page X XEUR BD Diagnostics Diagnostic Systems Catalog 2008/

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Katalog No Ürün Adı Amb. 274210 Acetate Differential Agar 500 g 212168 Actinomycete Isolation Agar 500 g 214010 Agar 500 g 281230 Agar,

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