Hollow Fiber Cleaning and Storage Procedure
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1 BioSeparations WaterSep BioSeparations Corporation 420 Maple Street, Suite 1 Marlborough, Mass Hollow Fiber Cleaning and Storage Procedure
2 HF Element Cleaning & Storage Procedure 1. Pre-CIP Procedure Pre-Flush Element with Buffer and Water 1.1. Establish and set process conditions Add buffer to feed vessel Recommended Feed Volume: Open feed, retentate and permeate valves completely Direct retentate and permeate streams to waste Flush feed - retentate process stream with buffer (recommended option) Turn pump on and increase pump speed to a feed flow rate (Q F ) that will Flush feed retentate and permeate stream to waste with total buffer volume Flush feed - retentate process stream with water Add water to feed vessel Recommended Feed Volume: liters per m 2 (1 2 liters per ft 2 ) Recommended Feed Temperature: 25 o C (minimum) 45 o C (preferred) Turn pump on and increase pump speed to a feed flow rate (Q F ) that will Flush feed retentate and permeate stream to waste with total water volume Drain water from system
3 2. CIP Procedure Clean Membrane Element with Caustic / Oxidant Solution 2.1. Establish and set process conditions Add cleaning solution to feed vessel. Recommended CIP Volume: Recommended Cleaning Solution & Conditions: 0.5N NaOH at o C (for moderate foulants) or 0.5N NaOH + 400ppm NaOCl at o C (for difficult foulants) Open feed, retentate and permeate valves completely Direct retentate and permeate back to feed vessel Clean-In-Place (CIP) Turn pump on and increase pump speed to a feed flow rate (Q F ) that will After 5 10 minutes, close permeate valve Continue to recirculate feed retentate stream back to feed vessel. Recommended CIP Time: minutes Drain cleaning agent from feed vessel and flush vessel to hold clean water.
4 3. Post-CIP Procedure Flush & Remove Cleaning Agents from Membrane Element 3.1. Establish and set process conditions Add water to feed vessel Required Volume to Flush Feed-Retentate: Recommend Volume to Flush Permeate: Recommended Flush Temperature: 25 o C (minimum) 45 o C (preferred) Open feed, retentate and permeate valves completely Direct retentate and permeate streams to waste Flush feed retentate stream Turn pump on and increase pump speed to a feed flow rate (Q F ) that will Flush feed retentate stream with total feed volume to drain Flush permeate stream Open permeate valve completely and directed to waste Lower feed flow rate (Q F ) and adjust retentate pressure to deliver a TMP of 3-15psig Flush permeate stream with total feed volume to drain
5 4. Membrane Recovery Determine Cleaning Effectiveness 4.1. Water Flux Rate During flushing of permeate stream (step ), measure the permeate water flux rate Set TMP between 8 15 psig (with permeate completely open) and measure the permeate water flow rate Normalized Water Permeability (NWP) Calculate the normalized water permeability (NWP) after cleaning by using the following equation: Normalized Water Permeability: [Filtrate Flux Rate / TMP] x Temperature Correction Factor 4.3. Membrane Recovery Compare the calculated NWP after cleaning to the pre-use clean NWP Calculate % membrane recovery using the following equation: % Membrane Recovery: [NWP after CIP / NWP pre-use ] x 100% If membrane recovery is below desired target, repeat cleaning. Extend CIP time if necessary. Recommended Membrane Recovery: 80% - 90% (minimum) 90% - 100% (preferred)
6 5. Storage Procedure Flush & Retain Membrane Element with Caustic 5.1. Establish and set process conditions Add 0.1N NaOH to feed vessel Required Volume: Open feed, retentate and permeate valves completely Direct retentate to feed vessel Direct permeate to waste 5.2. Flush system Turn pump on and increase pump speed to a feed flow rate (Q F ) that will generate a shear rate between sec Adjust retentate valve to generate a TMP of 3-15 psig Flush system for approximately 5 10 minutes Stop pump 5.3. Shut down system Close feed, retentate and permeate valves Remove and discard caustic storage solution
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