Roger Revilla i Domingo

Transcription

1 Roger Revilla i Domingo Schüttelstrasse 77 A / Top Vienna, AUSTRIA Phone: (+43) [email protected] Education : PhD in Biochemistry and Molecular Biophysics: cis-regulatory Analysis of the Sea Urchin delta Gene: Validating the Architecture of the Gene Regulatory Network Model for Micromere Lineage Specification. Supervised by Norman Chandler Professor of Cell Biology Eric H. Davidson : Master of Science in Biochemistry and Molecular Biophysics. Supervised by Norman Chandler Professor of Cell Biology Eric H. Davidson : Finish (Fourth year) Master in Science with First Class Honours in Physics. University of Bristol, Bristol, UK. Please note: the first three years of this degree are identical to the Bsc undergraduate degree in Physics : Third year of Master in Science in Physics at University of Bristol and Third year of Physics degree at Universitat Autonoma de Barcelona. University of Bristol, Bristol, UK, and Universitat Autonoma de Barcelona, Barcelona, Spain : Second year of Master in Science in Physics at University of Liverpool and Second year of Physics degree at Universitat Autonoma de Barcelona. University of Liverpool, Liverpool, UK, and Universitat Autonoma de Barcelona, Barcelona, Spain : First year of Physics degree at Universitat Autonoma de Barcelona. Universitat Autonoma de Barcelona, Barcelona, Spain.

2 Research Experience Starting 2012: VIPS Independent Postdoctoral Fellow. Max F. Perutz Laboratories, Vienna, Austria. Independent Postdoc of the Vienna International Postdoctoral Program (VIPS), associated with the lab of Dr. Florian Raible. The VIPS program supports young researchers in establishing themselves as independent investigators. The goal of my project is to investigate the evolution of stem cell gene regulatory networks. As the first step towards this goal, at present I am establishing a sponge stem cell system for gene regulatory network analysis : Marie-Curie and EMBO Postdoctoral Fellow. Institute of Molecular Pathology, Vienna, Austria. In the laboratory of Dr. Meinrad Busslinger, I contributed to the elucidation of the gene regulatory network that controls B cell development in the mouse. The elucidated network was based on genome-wide analyses (RNA-seq, ChIP-seq, and others) performed on mouse primary cells. In particular, I was the first to apply the RNA-seq technique at the Institute of Molecular Pathology, and therefore I established the experimental protocol and bioinformatics methods to analyze the data. I also performed ChIP-seq and CAGE-seq (Cap Analysis of Gene Expression), and devised computational methods to integrate the different genome-wide techniques to extract relevant gene regulatory network information (see [2] under Publications ) : PhD student in Biochemistry and Molecular Biophysics. Supervised by Norman Chandler Professor of Cell Biology Eric H. Davidson. I collaborated in the elucidation of the gene regulatory network that controls the early development of the sea urchin embryo. In particular, I developed quantitative techniques that are being used in cisregulatory analysis, I studied the transcriptional control of one of the genes involved in the regulatory network, and I identified a repressor transcription factor with a key role in the network (see [3] and [4] under Publications ). The development of this gene network represented the first of its kind (see [5], [6] and [7] under Publications ) : Independent Research Associate in collaboration with Ecole Normale Superieure de Cachan, France. Research project on clustering of a molecular dynamics trajectory with a Hamming distance, T.T.S.A.C, Manresa, Spain. Supervised by Dr. Jacques Gabarro-Arpa (CR1, CNRS), from Ecole Normale Superieure de Cachan, France. Based on the properties of discrete point arrangements we introduced the concept of dynamical activity, which allows one to formulate a simple double criterion for locating clusters of homologous conformers in a molecular dynamics trajectory. This collaboration resulted in Publication [8], which has served as the foundation for other works (Laboulais et al. 2002; Proteins. May 1;47(2):169-79). June 1997-August 1997: Nuffield Foundation Undergraduate Bursary funded research project on nanolithography. University of Bristol, Bristol, UK. Supervised by Professor Walther Schwarzacher. The research project consisted of using the Scanning Tunneling Microscope (STM) and the Atomic Force Microscope (AFM) among other techniques in order to study the physical properties of electrochemically deposited ultrathin films of organic materials on several substrates. This project was part of a larger effort devoted to the use of electrochemical deposition to prepare ultrathin films and nanostructures with unique physical properties.

3 : Research project on microbiology funded by Consejo Superior de Investigacion y Ciencia (CSIC) of Spain. Camp d Aprenentatge del Bages, Manresa, Spain. Supervised by Dr. Josep M. Gasol, researcher of the Insistut de Ciencies del Mar, CSIC, Barcelona, Spain. As a result of a research project carried out during my high school year ( Analysis of the microscopic life of the Agulla Lake and the Sequia River ), which was awarded a Premi CIRIT in 1993 (see under Honours and Awards ), the Spanish research organization Consejo Superior de Investigacion y Ciencia (CSIC) funded a continuation of the project, which consisted of a one year investigation of the microscopic life of the Agulla Lake. Publications: 1) Hoilova J., Mateasik A., Revilla-i-Domingo R., Raible F., Chorvat D. Jr., and Marcek Chorvatova A. (Submitted). Fingerprinting of metabolic states by NAD(P)H fluorescence lifetime spectroscopy in living cells: a review. Review. 2) Revilla-i-Domingo R., Bilic I., Vilagos B., Ebert A., Minich M., Tagoh H., Sommer A., Tamir I., Jaritz M. and Busslinger M. (2012) The B-cell identity factor Pax5 regulates distinct transcriptional programmes in early and late B lymphopoiesis. EMBO J. 31(14): ) Revilla-i-Domingo R., Oliveri P. and Davidson E.H. (2007). A missing link in the sea urchin embryo gene regulatory network: hesc and the double-negative specification of micromeres PNAS 104(30): ) Revilla-i-Domingo R., Minokawa T. and Davidson E.H. (2004). R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres. Dev. Biol. 274(2): ) Revilla-i-Domingo R. and Davidson E.H. (2003). Developmental Gene Network Analysis. Int. J. Dev. Biol. 47(7-8): Review. 6) Davidson EH, Rast JP, Oliveri P, Ransick A, Calestani C, Yuh CH, Minokawa T, Amore G, Hinman V, Arenas-Mena C, Otim O, Brown CT, Livi CB, Lee PY, Revilla R, Schilstra MJ, Clarke PJ, Rust AG, Pan Z, Arnone MI, Rowen L, Cameron RA, McClay DR, Hood L, Bolouri H. (2002). A provisional regulatory gene network for specification of endomesoderm in the sea urchin embryo. Dev. Biol. 246(1): ) Davidson EH, Rast JP, Oliveri P, Ransick A, Calestani C, Yuh CH, Minokawa T, Amore G, Hinman V, Arenas-Mena C, Otim O, Brown CT, Livi CB, Lee PY, Revilla R, Rust AG, Pan Z, Schilstra MJ, Clarke PJ, Arnone MI, Rowen L, Cameron RA, McClay DR, Hood L, Bolouri H. (2002). A genomic regulatory network for development. Science 295: Review. 8) Gabarro-Arpa J, Revilla R. (2000). Clustering of a molecular dynamics trajectory with a Hamming distance. Comput. Chem. 24(6): ) Revilla R. and Santamaria T. (1995). Microscopic Life in Agulla Lake. Dovella 51:23-28.

4 Presentations March 2011: Invited Speaker. Center for Genomic Regulation (CRG), Barcelona, Spain. Elucidating the Gene Regulatory Network that Controls B cell Development in the Mouse. November 2010: Poster Presentation. EMBO conference From Functional Genomics to Systems Biology, Heidelberg, Germany. Elucidating the Gene Regulatory Network that Controls B cell Development in the Mouse. March 2009: Poster Presentation. Cold Spring Harbor Laboratory meeting on Systems Biology, Cold Spring Harbor, USA, Elucidating the Gene Regulatory Network that Controls B cell Development in the Mouse. January 2007: Invited Speaker. Stanford University, Stanford, USA. December 2006: Invited Speaker. Massachusetts Institute of Technology (MIT), Boston, USA. October 2006: Invited Speaker. Institute of Molecular Pathology (IMP), Vienna, Austria. October 2006: Invited Speaker. Cambridge University, Cambridge, Regne Unit. October 2006: Invited Speaker. Princeton University, Princeton, USA. June 2005: Invited Speaker. Southampton University, Southampton, UK. A Gene Regulatory Network: An Information Processing System that Controls Development. June 2005: Invited Speaker. Barcelona University. Barcelona. Spain. A Gene Regulatory Network for Development: Its Architecture and some Implications for Body Plan Evolution. April 2005: Invited Speaker. Developmental Biology of Sea Urchins XVI Conference. Woods Hole, MA, USA. Cis-regulatory Analysis of the Delta Gene and the Repressor of Micromeres. November 2004 : Speaker for Caltech Developmental Biology Discussion Group Seminar. Cis-regulatory Analysis of the Sea Urchin Delta Gene. February 2004: Poster Presentation. Gordon Conference on Molecular Evolution. Ventura, CA, USA. Cis-regulatory Analysis of the Sea Urchin Delta Gene. September 2003: Invited Speaker. Developmental Biology of Sea Urchins XIV Conference. Woods Hole, MA, USA. Cis-regulatory Analysis of the Sea Urchin Delta Gene.

5 November 2000: Seminar talk for Professor Eric Winfree s class on DNA Computation. Information Processing in Development. December 1995 : Invited Speaker. Universitat Politecnica de Catalunya, Manresa, Spain. Microscopic Life in Agulla Lake. Teaching Experience At present: Supervision of a Masters student: Friederike Steudle. Max F. Perutz Laboratories, Vienna, Austria : Supervised and instructed new members of Eric. H. Davidson s laboratory in general laboratory techniques. June 2005-August 2005: Co-ran a summer course on Gene Networks and Evolution for Caltech Summer Undergraduate Research Fellowship (SURF) students, as parallel training to their research projects. January 2005-April 2005: Teaching Assistant of an advanced course on Quantitative Biology : Private tutor for First and Second year Algebra and Calculus courses of the Computer Science undergraduate degree at the Universitat Oberta de Catalunya. Barcelona, Spain : Secondary School teacher in Barcelona, Spain. I taught classes of Physics, Chemistry, Biology and Technology. Barcelona, Spain. Scientific Community Service Official reviewer for the journal Developmental Biology. October 2005: Biolunch seminar for Caltech biology community. California Institute of Technology. Pasadena, CA, USA. April 2004: Biolunch seminar for Caltech biology community. California Institute of Technology. Pasadena, CA, USA. Honours and Awards 2010: EMBO Long-Term Fellowship. Institute of Molecular Pathology, Vienna, Austria. 2008: Marie Curie Intra-European Fellowship for Career Development. Institute of Molecular Pathology, Vienna, Austria.

6 1997: Nuffield Foundation Undergraduate Research Bursary in Science. University of Bristol, Bristol, UK. See under Research Experience. 1997: Outstanding Student recognition letter from the Board of the Faculty of Science of the University of Bristol. University of Bristol, Bristol, UK. 1993: Winner of the Olimpiada Matematica de Catalunya. Catalonia. Spain. Mathematical Competition, open to high school students of all schools in the Autonomic Community of Catalonia, Spain. 1993: Premi CIRIT. Catalonia, Spain. Research Award for high school students. Awarded by the President of Catalonia, Spain, for the microbiology research project entitled Analysis of the microscopic life of the Agulla Lake and the Sequia River, the results of which were published in Publication [7]. 1992: Premi CIRIT. Catalonia, Spain. Research Award for high school students. Awarded by the President of Catalonia, Spain, for the mathematics research project entitled The Polinomyal Numbers : an original method for expanding polynomials of any number of terms and any degree. Other Qualifications Certificate of Lecturer by the Agència per a la Qualitat del Sistema Universitari de Catalunya (AQU Catalunya). Languages Catalan: 1 st native. Spanish: 2 nd native. English: Fluent. German: Proficient. French: Basic.