Institutional Animal Care and Use Committee. Rodent Survival Surgery Guidance

Size: px
Start display at page:

Download "Institutional Animal Care and Use Committee. Rodent Survival Surgery Guidance"

Transcription

1 Institutional Animal Care and Use Committee This guidance document includes tables of information and references as a resource for PIs. Please review the Brown University Institutional Animal Care and Use Committee (IACUC) Rodent Survival Surgery Policy for more information. TABLE - RECOMMENDED HARD SURFACE DISINFECTANTS* (e.g., table tops, equipment) AGENT EXAMPLES ** COMMENTS Alcohols 70% ethyl alcohol 85% isopropyl alcohol Contact time required is 5 minutes. Contaminated surfaces take longer to disinfect. Remove gross contamination before using. Quaternary Ammonium Roccal, Quatricide Tec-Surf II Sodium hypochlorite (Clorox 0% solution) dioxide (Clidox, Alcide, MB-0 ) Dispatch (Cidex, Cetylcide, Cide Wipes ) Rapidly inactivated by organic matter. Compounds may support growth of gram negative bacteria. Corrosive. Presence of organic matter reduces activity. dioxide must be fresh; kills vegetative organisms within 3 minutes of contact. Note: chlorox solutions need to be made up fresh daily to maintain activity, whereas Dispatch, available from Environmental Services, is labeled as stable for year. Rapidly disinfects surfaces. Phenolics Lysol, TBQ Less affected by organic material than other disinfectants. Chlorhexidine Nolvasan, Hibiclens Presence of blood does not interfere with activity. Rapidly bactericidal and sistent. Effective against many viruses. *Always follow manufacturer's instructions for dilution and expiration iods. **The use of common brand names as examples does not indicate a product endorsement. Version, 3/0

2 TABLE 2 - SKIN DISINFECTANTS * Iodophors Cholorhexidine Betadine, Prepodyne, Wescodyne Nolvasan, Hibiclens Reduced activity in presence of organic matter. Wide range of microbicidal action. Works best in ph 6-7. Presence of blood does not interfere with activity. Rapidly bactericidal and sistent. Effective against many viruses. Excellent for use on skin. Alternating disinfectants is more effective than using a single agent. For example, an iodophor scrub (with soap) can be alternated once with 70% alcohol, followed by a final soaking with a disinfectant solution (without soap). Alcohol, by itself, is not an adequate skin disinfectant. Since the evaporation of alcohol can induce hypothermia in small animals, avoid exposing excessively large areas. *Always follow manufacturer's instructions for dilution and expiration iods. **The use of common brand names as examples does not indicate a product endorsement. TABLE 3 - PROCEDURES FOR STERILIZING SURGICAL INSTRUMENTS * --RECOMMENDED METHODS FOR STERILIZING SURGICAL INSTRUMENTS-- Steam sterilization (moist heat) Dry Heat Autoclave Hot Bead Sterilizer Dry Chamber Effectiveness dependent upon temature, pressure and time (e.g., 2 o C for 5 min. vs. 3 o C for 3 min.). Fast. Instruments must be cooled before contacting tissue. Only tips of instruments are sterilized with hot beads. Instruments should be cleaned prior to inserting in the hot bead sterilizer. Replace the beads the manufacture s recommendations. --ALTERNATIVE METHODS FOR STERILIZING SURGICAL INSTRUMENTS-- Gas Sterilization Ethylene Oxide Requires 30% or greater relative humidity for effectiveness against spores. Gas is irritating to tissue; all materials require safe airing time. Dioxide Corrosive to instruments. Instruments must be rinsed with a minimum of (3) fresh washes of sterile saline or sterile water before use. Hydrogen Peroxide- Acetic Acid Glutaraldehyde (Cidex, Cetylcide, Metricide ) Actril Spor-Klenz Several hours required for sterilization. Corrosive and irritating. Instruments must be rinsed with a minimum of (3) fresh washes of sterile saline or sterile water before use. Several hours required for sterilization. Corrosive and irritating. Instruments must be rinsed with a minimum of (3) fresh washes of sterile saline or sterile water before use. * Always follow manufacturer's instructions for dilution, exposure times and expiration iods. ** The use of common brand names as examples does not indicate a product endorsement. Version, 3/0

3 TABLE 4 - RECOMMENDED INSTRUMENT DISINFECTANTS * Chlorhexidine Sodium hypochlorite (Clorox 0% solution) dioxide (Clidox, Alcide ) Nolvasan Hibiclens Corrosive to instruments. Presence of organic matter reduces activity. dioxide must be fresh. Kills vegetative organisms within 3 min. Instruments must be rinsed with a minimum of (3) fresh washes of sterile saline or sterile water before use. Presence of blood does not interfere with activity. Rapidly bactericidal and sistent. Effective against many viruses. Instruments must be rinsed with a minimum of (3) fresh washes of sterile saline or sterile water before use. *Always follow manufacturer's instructions for dilution, exposure times and expiration iods. ** The use of common brand names as examples does not indicate a product endorsement. TABLE 5 - WOUND CLOSURE SELECTION MATERIAL* CHARACTERISTICS AND FREQUENT USES Polyglactin 90 (Vicryl ), Polydiaxanone (PDS ), or Polyglyconate (Maxon ) Polypropylene (Prolene ) Nylon (Ethilon ) Silk Chromic Gut Stainless Steel Wound Clips, Staples Cyanoacrylate (Vetbond, Nexaband ) Absorbable; days. Ligate or suture tissues where an absorbable suture is desirable. Absorbable; 6 months. Ligate or suture tissues especially where an absorbable suture and extended wound support is desirable Non-absorbable. Inert. Non-absorbable. Inert. General skin closure. Non-absorbable. Restrict the use of silk to cardiovascular procedures or where silk s excellent handling proties are critical. Avoid for such purposes as routine skin closure since it may wick microorganisms into the wound, and cause tissue reactivity. Absorbable. Versatile material. Non-absorbable. Requires instrument for removal. Skin glue. For non-tension bearing wounds. Note: use only products labeled for surgical use, su glue is not acceptable for surgery. Suture gauge selection: Use the smallest gauge suture material that will form adequately. Cutting and reverse cutting needles: Provide edges that will cut through dense, difficult to penetrate tissue, such as skin. Non-cutting, ta point or round needles: Have no edges to cut through tissue; used primarily for suturing easily torn tissues such as itoneum or intestine. * The use of common brand names as examples does not indicate a product endorsement. TABLE 6 ANESTHESIA Version, 3/0

4 Inhaled anesthetics: Isoflurane delivered by mask or endotracheal tube via a precision vaporizer is recommended for all species. Vaporizers are available for use in Ship St. and Biomed procedure rooms. Please contact ACF for information regarding vaporizer availability and training. For very brief procedures, (e.g., tail biopsies for genotyping), it may be acceptable to use isoflurane or other inhalant anesthetics, without a precision vaporizer, in a bell-jar placed in a fume hood while precluding direct contact of animal skin with inhalant anesthetics. In all cases the anesthetic vapors must be adequately vented to prevent inadvertent exposure of sonnel. Injectable anesthetics: Injectable anesthetics are appropriate for many procedures. There is, however, a great deal of variation in depth and duration of anesthesia among rodent strains and individual animals. ANESTHETIC MOUSE RAT GUINEA PIG Ketamine/ Dexmedetomidine Cocktail Ketamine/ Xylazine Cocktail 2 75 mg kg - i/p 0.5 mg kg - i/p SQ or IP, may not produce surgical plane of anesthesia in some mice 75 mg kg - i/p 0.25 mg mg kg - i/p mg kg - i/p 3 mg kg - i/p 40 mg kg - i/p 0.25 mg mg kg - i/p 35 mg kg mg kg - Pentobarbital mg kg - i/p mg kg - i/p 28 mg kg - IP The 2 components can be mixed with water for injection (WFI) (USP water) see table for Rodent Cocktail Solutions below. 2 Xylazine is a potent respiratory depressant. Supplemental dosing, if necessary, should be done with ½ the original dose of ketamine alone. 3 Consider replacing with inhalant or another injectable anesthetic. Consult veterinarians for information. TABLE 7 RODENT COCKTAIL SOLUTIONS MOUSE RAT GUINE A PIG MOUSE RAT GUINEA PIG Version, 3/0

5 Ketamine ml (00 mg ml - ) 3.75 ml 3.75 ml Ketamine ml (00 mg ml - ) 0.5 ml 3.75 ml 2.0 ml Xylazine 2.5 ml 2.5 ml Dexmedetomidine 0.5 ml 2.5 ml 2.5mL (0 mg ml - ) (0.5 mg ml - ) WFI 3.75 ml 3.75 ml WFI 4.0 ml 3.75 ml 5.5 ml Total Volume 0.0 ml 0.0 ml Total Volume 5.0 ml 0.0 ml 0.0 ml Dosage 0.2 ml 00gm 0.2 ml 00gm Dosage 0. ml 0 gm 0.2 ml 00gm 2.0mL kg Consult veterinarians for information. TABLE 8 - ANALGESICS/ANTI-INFLAMMATORY AGENTS * AGENT MOUSE RAT GUINEA PIG Buprenorphine mg kg - SQ 0.05 mg kg - SQ 0.05 mg kg - SQ Every 2 hours Every 2 hours Every 2 hours Butorphanol mg kg - SQ 2 mg kg - SQ Every 4 hours Every 4 hours Meloxicam 0.3 mg kg - PO mg kg - PO 0.2 mg kg - PO Carprofen 5 mg kg - SQ Every 24 hours 5 mg kg - SQ Every 24 hours * The IACUC must approve the exclusion of analgesics/anti-inflammatory agents for eximental surgery. If you feel that these agents are likely to interfere with the objectives of your research, provide a scientific justification for their exclusion in your protocol. Consult veterinarians for information. References: NRC (National Research Council), Institute of Laboratory Animal Resources, Commission on Life Sciences Guide for the Care and Use of Laboratory Animals, Washington D.C., National Academy Press. The Animal Welfare Act, 7USC Sec 23 et seq. Title 9, Code of Federal Regulations, parts, 2, & 3, January 998. Laboratory Animal Anesthesia, A practical introduction for research workers and technicians., 2 nd Edition, P.A. Flecknell (996) Version, 3/0

Recommendations for Aseptic Technique and Post- Operative Care for Rodent Surgery

Recommendations for Aseptic Technique and Post- Operative Care for Rodent Surgery Recommendations for Aseptic Technique and Post- Operative Care for Rodent Surgery It is the responsibility of the veterinary staff, investigator, laboratory animal technicians, and the facility manager

More information

Guidelines for Survival Rodent Surgery

Guidelines for Survival Rodent Surgery Guidelines for Survival Rodent Surgery Scope: These guidelines apply to all surgical procedures performed on rodents at the NIH in which the animals are expected to recover from anesthesia. 1 Prior to

More information

PACUC Guidelines. Rodent Survival Surgery

PACUC Guidelines. Rodent Survival Surgery PACUC Guidelines Rodent Survival Surgery Guidelines for rodent survival surgery are based on the National Research Council Guide for the Care and Use of Laboratory Animals 8 th edition and the Animal Welfare

More information

Animal Care and Use Program Policy & Procedures Rodent Surgery. To outline the proper procedures for the performance of rodent surgery

Animal Care and Use Program Policy & Procedures Rodent Surgery. To outline the proper procedures for the performance of rodent surgery Animal Care and Use Program Policy & Procedures Rodent Surgery Objective: Author: To outline the proper procedures for the performance of rodent surgery Chandra D. Williams, DVM, DACLAM Reviewed: 09/24/2012

More information

Guidelines for Survival Rodent Surgery

Guidelines for Survival Rodent Surgery Guidelines for Survival Rodent Surgery These guidelines apply to all surgical procedures performed on rodents at Brooklyn College-CUNY in which the animals are expected to recover from anesthesia. Prior

More information

GUIDELINES: Aseptic Technique for Rodent Survival Surgeries Surgical Area Animal Preparation 1.

GUIDELINES: Aseptic Technique for Rodent Survival Surgeries Surgical Area Animal Preparation 1. GUIDELINES: Aseptic Technique for Rodent Survival Surgeries The following Guidelines are provided to ensure correct aseptic technique for all rodent survival surgeries. Surgical Area 1. The surgical area

More information

IACUC Policy on Survival Rodent Surgery

IACUC Policy on Survival Rodent Surgery IACUC Policy on Survival Rodent Surgery References: ILAR Guide for the Care and Use of Laboratory Animals: http://oacu.od.nih.gov/regs/guide/guide.pdf NIH OACU ARAC Guidelines: http://oacu.od.nih.gov/arac/documents/rodent_surgery.pdf

More information

Arizona State University Institutional Animal Care and Use Committee STANDARD INSTITUTIONAL GUIDELINE RODENT SURGERY

Arizona State University Institutional Animal Care and Use Committee STANDARD INSTITUTIONAL GUIDELINE RODENT SURGERY Arizona State University Institutional Animal Care and Use Committee STANDARD INSTITUTIONAL GUIDELINE RODENT SURGERY It is the policy of the IACUC that all survival surgeries involving rodents be conducted

More information

ANIMAL SURGERY (ASEPTIC TECHNIQUE AND DISINFECTANTS)

ANIMAL SURGERY (ASEPTIC TECHNIQUE AND DISINFECTANTS) Duke University & Duke University Medical Center Animal Care & Use Program Policy ANIMAL SURGERY (ASEPTIC TECHNIQUE AND DISINFECTANTS) PERFORMANCE STANDARD: All animal procedures will include appropriate

More information

STANDARD OPERATING PROCEDURE #201 RODENT SURGERY

STANDARD OPERATING PROCEDURE #201 RODENT SURGERY STANDARD OPERATING PROCEDURE #201 RODENT SURGERY 1. PURPOSE The intent of this Standard Operating Procedure (SOP) is to describe procedures for survival rodent surgery. 2. RESPONSIBILITY Principal investigators

More information

RODENT SURVIVAL SURGERY

RODENT SURVIVAL SURGERY Institutional Animal Care Program Title: RODENT SURVIVAL SURGERY Policy#: IACP 004 Date in Effect: 12/14/07 Rev #: 04 Rev Date: 12/13/13 In Effect Rescinded Date Rescinded: A) PURPOSE In accordance with

More information

Surgical procedures conducted at field sites need not be performed in dedicated facilities, but must be performed using aseptic procedures.

Surgical procedures conducted at field sites need not be performed in dedicated facilities, but must be performed using aseptic procedures. UNMC/UNO ANIMAL CARE AND USE PROGRAM POLICIES AND PROCEDURES APPROVAL DATE: 10/16/2013 TITLE: Policy for Surgery PAGE 1 OF 5 POLICY Successful surgical outcomes depend on appropriate pre-surgical planning,

More information

Decontamination and Waste Management www.biosecurity.sandia.gov

Decontamination and Waste Management www.biosecurity.sandia.gov Decontamination and Waste Management www.biosecurity.sandia.gov SAND No. 2006-3684C Sandia is a multiprogram laboratory operated by Sandia Corporation, a Lockheed Martin Company, for the United States

More information

CHAPTER V: DISPOSAL OF WASTES CONTAMINATED WITH INFECTIOUS AGENTS

CHAPTER V: DISPOSAL OF WASTES CONTAMINATED WITH INFECTIOUS AGENTS CHAPTER V: DISPOSAL OF WASTES CONTAMINATED WITH INFECTIOUS AGENTS These biohazard waste disposal guidelines are designed to not only protect the public and the environment, but also laboratory and custodial

More information

Standard Operating Procedure

Standard Operating Procedure Animal Care and Use Program Standard Operating Procedure Title: Standard Surgical Practices for Rodent Recovery Surgery SOP NO: AC-G004 Supersedes Version: L1 & L2 Effective Date: August 27, 2010 Choose

More information

STERILIZATION AND DISINFECTION

STERILIZATION AND DISINFECTION STERILIZATION AND DISINFECTION Importance of hand washing shown by Semmelweis STERILIZATION A physical or chemical process that destroys or eliminates all forms of microbial life, including spores. A satisfactory

More information

IX. Decontamination and Spills

IX. Decontamination and Spills IX. Decontamination and Spills IX.1. Definitions Sterilization: the act or process, physical or chemical, which destroys or eliminates all forms of life, especially microorganisms. Decontamination: reduction

More information

Professional Certification in Biological Waste Management Examination Content, Sample Questions & References

Professional Certification in Biological Waste Management Examination Content, Sample Questions & References Professional Certification in Biological Waste Management Examination Content, Sample Questions & References The IFBA s Professional Certification (PC) in Biological Waste Management identifies individuals

More information

STERILIZATION د STERILIZATION

STERILIZATION د STERILIZATION STERILIZATION. د STERILIZATION Is a term referring to any process that eliminates (removes) or kills all forms of microbial life, including transmissible agents (such as fungi, bacteria, viruses, spores

More information

Protocol for Disinfection of Cell Culture and Tissue Culture in Media:

Protocol for Disinfection of Cell Culture and Tissue Culture in Media: Protocol for Disinfection of Cell Culture and Tissue Culture in Media: Location: Hickory Hall 001 Director: Dr. Guido Verbeck DECONTAMINATION OF CELL CULTURE WASTE Cell culture has become a common laboratory

More information

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS

GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS GUIDELINES FOR THE PRODUCTION OF ANTIBODIES IN LABORATORY ANIMALS Table of Contents 1. Purpose 2. Choice of Species and Strain 3. Immunizing Antigen 4. Procedures for Polyclonal Antibody Production 5.

More information

Infection Prevention + Control

Infection Prevention + Control Physiotherapy Alberta regulates and leads the practice of physiotherapy in Alberta. Contact us for more information on this or other position statements. 780.438.0338 1.800.291.2782 info@physiotherapyalberta.ca

More information

position statement INFECTION CONTROL INTRODUCTION POSITION STATEMENT PERFORMANCE EXPECTATIONS

position statement INFECTION CONTROL INTRODUCTION POSITION STATEMENT PERFORMANCE EXPECTATIONS INFECTION CONTROL INTRODUCTION Appropriate infection control is an essential element of clinical practice management based on its importance to the health and safety of patients, practitioners and the

More information

3M TM DuraPrep TM Surgical Solution (Iodine Povacrylex [0.7% available iodine] and Isopropyl Alcohol, 74% w/w) Patient Preoperative Skin Preparation

3M TM DuraPrep TM Surgical Solution (Iodine Povacrylex [0.7% available iodine] and Isopropyl Alcohol, 74% w/w) Patient Preoperative Skin Preparation 3M TM DuraPrep TM Surgical Solution (Iodine Povacrylex [0.7% available iodine] and Isopropyl Alcohol, 74% w/w) Patient Preoperative Skin Preparation Commonly Asked Questions I like the concept of a one-step

More information

KGI MEDICAL WASTE MANAGEMENT PLAN

KGI MEDICAL WASTE MANAGEMENT PLAN 1revised 2012 I. Facility Information KGI MEDICAL WASTE MANAGEMENT PLAN a. Contact Person: Barbara Erwin Director Research Operations/Chemical and Biological Laboratory Safety/Chair. 909-607-0160 i. Facility:

More information

1-800-862-2731 Administration of Meropenem For Child

1-800-862-2731 Administration of Meropenem For Child 1 Drug Name: _Meropenem 1-800-862-2731 Administration of Meropenem For Child Dosage: milligrams every hours over 5 minutes Key Points: FLUSHING: Meropenem Heparin flush 1. Always wash your hands with an

More information

GUIDELINES FOR SURGICAL PROCEDURES

GUIDELINES FOR SURGICAL PROCEDURES GUIDELINES FOR SURGICAL PROCEDURES Table of Contents 1) Background 2) Classification of Surgical Procedures 3) Multiple Survival Surgeries 4) Training 5) Surgery and Monitoring a) Patient Preparation b)

More information

"ADOPTED STANDARDS FOR THE REGULATION OF MEDICAL WASTE" IN HEALTH CARE FACILITIES LICENSED BY THE MISSISSIPPI STATE DEPARTMENT OF HEALTH

ADOPTED STANDARDS FOR THE REGULATION OF MEDICAL WASTE IN HEALTH CARE FACILITIES LICENSED BY THE MISSISSIPPI STATE DEPARTMENT OF HEALTH "ADOPTED STANDARDS FOR THE REGULATION OF MEDICAL WASTE" IN HEALTH CARE FACILITIES LICENSED BY THE MISSISSIPPI STATE DEPARTMENT OF HEALTH REGULATED MEDICAL WASTE "Infectious medical wastes" includes solid

More information

IACUC Guideline RODENT ANESTHESIA & ANALGESIA FORMULARY

IACUC Guideline RODENT ANESTHESIA & ANALGESIA FORMULARY Background This guideline is designed to provide a single source of information for investigators that use rodents models of biomedical disease and discovery. The following tables reference contemporary

More information

Steam Sterilization Cycles for Lab Applications

Steam Sterilization Cycles for Lab Applications Steam Sterilization Cycles for Lab Applications Presented by Gary Butler STERIS Life Sciences August 2009 Early Steam Sterilizers Koch Upright Sterilizer First Pressurized Sterilizer First built by Pasteur-Chamberlain

More information

Sutures and needles. Sutures

Sutures and needles. Sutures Sutures and needles Sutures A wide variety of material is available for suturing and ligating tissues. Sutures are selected for use according to the required function. For example, arteries are sutured

More information

Oxivir Tb and Accelerated Hydrogen Peroxide (AHP) Frequently Asked Questions

Oxivir Tb and Accelerated Hydrogen Peroxide (AHP) Frequently Asked Questions Oxivir Tb and Accelerated Hydrogen Peroxide (AHP) Frequently Asked Questions What is Accelerated Hydrogen Peroxide (AHP)? AHP is a patented synergistic blend of commonly used, safe ingredients that when

More information

Appendix J IBC Biohazard Spill Management Plan

Appendix J IBC Biohazard Spill Management Plan OVERVIEW Prevention is the most important part of any spill management plan. Be sure to read and understand standard operating procedures (SOP s) and protocols for safe manipulation of biohazards before

More information

IACUC Guideline LARGE ANIMAL FORMULARY

IACUC Guideline LARGE ANIMAL FORMULARY The intention behind the development of a University of Pennsylvania IACUC-endorsed drug formulary for the larger species used in biomedical research was to provide guidance for anesthetic and analgesic

More information

Surgery: Setup and Supplies

Surgery: Setup and Supplies THE SURGERY SUITE Surgeries other than routine neuters and treatment of abscesses are performed in the surgery suite. We keep a chart with the preferences of our various surgeons, including which side

More information

Fundamental Autoclave Techniques

Fundamental Autoclave Techniques Fundamental Autoclave Techniques Version 3, September 2010 Veronika Tatarinoff GSBmE University of NSW Brandy Nelson University of Kentucky The following information is directed at aseptic/sterile surgical,

More information

CLEANING AND SANITIZING

CLEANING AND SANITIZING CLEANING AND SANITIZING Cleaning and sanitizing procedures must be part of the standard operating procedures that make up your food safety program. Improperly cleaned and sanitized surfaces allow harmful

More information

FREQUENTLY ASKED QUESTIONS

FREQUENTLY ASKED QUESTIONS FREQUENTLY ASKED QUESTIONS 1. What are all these acronyms & abbreviations? 2. What is the Animal Welfare Assurance Number for Rosalind Franklin University for grant submission? 3. Do I need an Animal Protocol?

More information

33 Infection Control Techniques

33 Infection Control Techniques CHAPTER 33 Infection Control Techniques Learning Outcomes 33.1 Describe the medical assistant s role in infection control. 33.2 Describe methods of infection control. 33.3 Compare and contrast medical

More information

Ethylene Oxide (EtO or EO) The Future Of Gas Sterilization

Ethylene Oxide (EtO or EO) The Future Of Gas Sterilization Ethylene Oxide (EtO or EO) The Future Of Gas Sterilization Currently the overwhelming demand for EtO comes from the chemical industry, where it is a very widely used monomer. The single biggest use for

More information

Administrative Procedure

Administrative Procedure Administrative Procedure Number: 410 Effective 1/30/98 Supercedes: 1 of Subject: MEDICAL WASTE MANAGEMENT PLAN 1.0. PURPOSE: The purpose of the California State University Los Angeles Medical Waste Management

More information

hypochlorites of milk plant apparatus, dairy equipment and

hypochlorites of milk plant apparatus, dairy equipment and EFFECTIVENESS OF HOT HYPOCHLORITES OF LOW ALKALINITY IN DESTROYING MYCOBACTERIUM TUBERCULOSIS S. M. COSTIGAN General Laboratories, Division of Pennsylvania Salt Philadelphia, Pennsylvania Manufacturing

More information

Standard Operating Procedure for Dacarbazine in Animals

Standard Operating Procedure for Dacarbazine in Animals Standard Operating Procedure for Dacarbazine in Animals 1. Health hazards Dacarbazine, also known as DTIC, DIC, and Imidazole Carboxamide, is an antineoplastic chemotherapy drug used in the treatment of

More information

Cleaning, Disinfection, and Sterilization. Basics of Infection Prevention 2-Day Mini-Course 2013

Cleaning, Disinfection, and Sterilization. Basics of Infection Prevention 2-Day Mini-Course 2013 Cleaning, Disinfection, and Sterilization Basics of Infection Prevention 2-Day Mini-Course 2013 2 Objectives Describe basic principles of cleaning, disinfection, sterilization Identify when to use cleaning,

More information

Training on Standard Operating Procedures for Health Care Waste Management Swaziland 12 May, 2011

Training on Standard Operating Procedures for Health Care Waste Management Swaziland 12 May, 2011 Training on Standard Operating Procedures for Health Care Waste Management Swaziland 12 May, 2011 Safe Infectious Waste Handling and Transport Objective Waste Overview Roles and Responsibilities of Waste

More information

Veterinary Anesthetic and Analgesic Formulary 3 rd Edition, Version G

Veterinary Anesthetic and Analgesic Formulary 3 rd Edition, Version G Veterinary Anesthetic and Analgesic Formulary 3 rd Edition, Version G I. Introduction and Use of the UC Denver Veterinary Formulary II. Anesthetic and Analgesic Considerations III. Species Specific Veterinary

More information

Surgical Capabilities

Surgical Capabilities Surgical Capabilities At Charles River, we understand how important high-quality surgical models that meet exacting scientific and regulatory standards are to your research. As the premiere provider of

More information

Basic Wound Closure & Knot Tying. Joslyn Albright, MD General Surgery PGY-4 Research Resident

Basic Wound Closure & Knot Tying. Joslyn Albright, MD General Surgery PGY-4 Research Resident Basic Wound Closure & Knot Tying Joslyn Albright, MD General Surgery PGY-4 Research Resident Objectives Provide basic information on commonly used suture materials Review general principles of wound closure

More information

Recommendations for the Safe Use of Handling of Cytotoxic Drugs

Recommendations for the Safe Use of Handling of Cytotoxic Drugs Recommendations for the Safe Use of Handling of Cytotoxic Drugs Introduction Cytotoxic drugs are toxic compounds and are known to have carcinogenic, mutagenic and/or teratogenic potential. With direct

More information

MATERIAL SAFETY DATA SHEET "LIQUID FIRE" DRAIN LINE OPENER 09/2007 PHYSICAL/CHEMICAL CHARACTERISTICS:

MATERIAL SAFETY DATA SHEET LIQUID FIRE DRAIN LINE OPENER 09/2007 PHYSICAL/CHEMICAL CHARACTERISTICS: 09/2007 MATERIAL SAFETY DATA SHEET "LIQUID FIRE" DRAIN LINE OPENER MANUFACTURER'S NAME: AMAZING PRODUCTS, INC. P.O. BOX 14226 LOUISVILLE, KY 40214 PHONE: 502-361-3655 FAX: 502-361-1810 E-MAIL: amazingp@bellsouth.net

More information

Detergent and disinfectant manufacturing worker

Detergent and disinfectant manufacturing worker International Hazard Datasheets on Occupation Detergent and disinfectant manufacturing worker Who are detergent and disinfectant manufacturing workers? They are industrial chemists who tend equipment units

More information

Feline Anesthesia Richard M. Bednarski, DVM, MSc The Ohio State University College of Veterinary Medicine Current Issues in Feline Anesthesia

Feline Anesthesia Richard M. Bednarski, DVM, MSc The Ohio State University College of Veterinary Medicine Current Issues in Feline Anesthesia Feline Anesthesia Richard M. Bednarski, DVM, MSc The Ohio State University College of Veterinary Medicine Current Issues in Feline Anesthesia Chemical Restraint Protocols Trap, Neuter, Release Anesthetic

More information

CHILDCARE SETTINGS. Childcare Settings

CHILDCARE SETTINGS. Childcare Settings Childcare Settings This document provides guidelines and recommendations for cleaning childcare settings such as nurseries, crèches and schools in the event of a disease outbreak in the vicinity. Cleaning

More information

Item 2 Guidelines for the Production of Polyclonal and Monoclonal Antibodies in Rodents and Rabbits

Item 2 Guidelines for the Production of Polyclonal and Monoclonal Antibodies in Rodents and Rabbits Antibody Production/Neoplasia Item 2 Guidelines for the Production of Polyclonal and Monoclonal Antibodies in Rodents and Rabbits Monoclonal and polyclonal antibodies are important reagents utilized in

More information

SECTION 1 - PRODUCT IDENTIFICATION

SECTION 1 - PRODUCT IDENTIFICATION Product Identification Physical Data Hazardous Components Fire and Explosion Hazard Data Reactivity Data Health Hazard Information Spill or Leak Procedures Handling Information Special Protection Information

More information

Dacarbazine for Injection, USP Dacarbazine Antineoplastic

Dacarbazine for Injection, USP Dacarbazine Antineoplastic MATERIAL SAFETY DATA SHEET SECTION 1 - PRODUCT & COMPANY IDENTIFICATION 1501 East Woodfield Road Suite 300 East Schaumburg, IL 60173 (847) 969-2700 Product Identifier: Chemical Name: Category: Emergency

More information

Guidelines for Spay/Neuter

Guidelines for Spay/Neuter Guidelines for Standards of Care in Animal Shelters Guidelines for Spay/Neuter Cynthia Barker Cox, DVM MSPCA Boston, MA Natalie Isaza, DVM College of Veterinary Medicine University of Florida Guidelines

More information

THE BASICS OF STERILIZATION

THE BASICS OF STERILIZATION THE BASICS OF STERILIZATION Objectives: State the importance of sterilization to patient care Review three key essentials of the sterilization process Describe sterilization methods used, application,

More information

Page: Page 1 of 7 Author: Kristin Smith MAL Director: Dr. Peggy Borum FSHN Chair: Dr. Neil Shay

Page: Page 1 of 7 Author: Kristin Smith MAL Director: Dr. Peggy Borum FSHN Chair: Dr. Neil Shay Page 1 of 7 Author: Kristin Smith MAL Director: Dr. Peggy Borum FSHN Chair: Dr. Neil Shay I PURPOSE The purpose of the preparation of surgery trays is to have instruments ready in the PNICU for sterile

More information

Biosafety Spill Response Guide

Biosafety Spill Response Guide Yale University Office of Environmental Health & Safety Biosafety Spill Response Guide Office of Environmental Health & Safety 135 College Street, 1 st Floor, New Haven, CT 06510 Telephone: 203-785-3550

More information

CONTROL: Any tissue containing acid-fast organisms. Use Millipore filtered water in the waterbath and staining procedure.

CONTROL: Any tissue containing acid-fast organisms. Use Millipore filtered water in the waterbath and staining procedure. SURGICAL PATHOLOGY - HISTOLOGY Date: STAINING MANUAL - MICROORGANISMS Page: 1 of 3 ACID-FAST BACTERIA - ZIEHL-NEELSEN STAIN (AFB) PURPOSE: Used in the demonstration of acid-fast bacteria belonging to the

More information

EH&S. Sheet. Fact. Safe and Effective Use of Autoclaves. What are autoclaves? Factors for effective sterilization. Dry heat cycle - when to use

EH&S. Sheet. Fact. Safe and Effective Use of Autoclaves. What are autoclaves? Factors for effective sterilization. Dry heat cycle - when to use Please post or circulate Fact heet nvironment, ealth and afety Information for the Berkeley Campus No. 33 Revised 04/04/11 afe and ffective Use of Autoclaves Autoclaves are easy to use but can pose a safety

More information

MATERIAL SAFETY DATA SHEET

MATERIAL SAFETY DATA SHEET MATERIAL SAFETY DATA SHEET SECTION 1 - CHEMICAL PRODUCT & COMPANY IDENTIFICATION Pfizer Consumer Healthcare Pfizer Inc 201 Tabor Road Morris Plains, New Jersey 07950 Emergency telephone Hours of operation

More information

Emergency Telephone (800) 551-7176

Emergency Telephone (800) 551-7176 MATERIAL SAFETY DATA SHEET SECTION 1 - PRODUCT & COMPANY IDENTIFICATION 1501 East Woodfield Road Suite 300 East Schaumburg, IL 60173 (847) 969-2700 Product Identifier: Chemical Name: Category: Mesna Detoxifying

More information

Physical Methods Of Microbial Control: 1. Heat: denatures proteins (enzymes) = death

Physical Methods Of Microbial Control: 1. Heat: denatures proteins (enzymes) = death Physical Methods Of Microbial Control: 1. Heat: denatures proteins (enzymes) = death A. Moist heat: disrupts H-bonds, coagulates molecules denatures proteins 1. Boiling: 100 C, 10 min Kills: vegetative

More information

AUTOCLAVE PROGRAM. SOP Bio-006 FOR THE USE OF AUTOCLAVE FOR STERILIZATION OF MATERIALS AND BIOLOGICAL WASTE SOP

AUTOCLAVE PROGRAM. SOP Bio-006 FOR THE USE OF AUTOCLAVE FOR STERILIZATION OF MATERIALS AND BIOLOGICAL WASTE SOP ENVIRONMENTAL AND EMERGENCY MANAGEMENT ENVIRONMENTAL HEALTH AND SAFETY 175 Cabot St. Wannalancit Suite 311 Lowell MA 01854 Ruth Medina, Ph.D. Tel: 978-934-2778 Senior Biosafety Specialist/Biosafety Officer

More information

CARE HOMES AND NURSING HOMES

CARE HOMES AND NURSING HOMES Care Homes and Nursing Homes: This document provides guidelines and recommendations for cleaning care homes and nursing homes in the event of a disease outbreak in the vicinity. Cleaning and disinfection

More information

Sterilization methods and equipment Lab 1-2

Sterilization methods and equipment Lab 1-2 Sterilization methods and equipment Lab 1-2 PHT 434 Sterilization Sterilization a process that by which all viable M.O are removed or destroyed, based on a probability function. Sterilization concept Sterilization

More information

SAFE HEALTHY CHILDREN A Health and Safety Manual for Childcare Providers

SAFE HEALTHY CHILDREN A Health and Safety Manual for Childcare Providers TABLE OF CONTENTS Chapter 7: Food Storage and Preparation Safe Food: Food Storage and Preparation 7.1 Requirements of the Day Nurseries Act... 7.2 Recommendations of the Medical Officer of Health... 7.2

More information

Selecting an Appropriate Sanitizer or Disinfectant

Selecting an Appropriate Sanitizer or Disinfectant Selecting an Appropriate Sanitizer or Disinfectant One of the most important steps in reducing the spread of infectious diseases in child care settings is cleaning, sanitizing or disinfecting surfaces

More information

Evaluating MSDS First Aid Advice

Evaluating MSDS First Aid Advice Evaluating MSDS First Aid Advice Senior Technical Specialist Evidence-Based Approach Identify specific questions Search for relevant literature Critically evaluate quality of evidence Determine best answer

More information

Infection Control in the Dental Practice through Proper Sterilization

Infection Control in the Dental Practice through Proper Sterilization Midmark Corporation 60 Vista Drive P.O. Box 286 Versailles, Ohio 45380-0286 937-526-3662 midmark.com Infection Control in the Dental Practice through Proper Sterilization In today's world, the need for

More information

3. Maintenance of Laboratory Equipment

3. Maintenance of Laboratory Equipment - 52 - Mainten: of Lab Equipment / National Guidelines SLCM SLCM National Guidelines / Mainte: of Lab Equipment - 53-3. Maintenance of Laboratory Equipment Introduction Equipment maintenance is one important

More information

SOP 001: POLYCLONAL ANTIBODY PRODUCTION IN RABBITS

SOP 001: POLYCLONAL ANTIBODY PRODUCTION IN RABBITS SOP 001: POLYCLONAL ANTIBODY PRODUCTION IN RABBITS PURPOSE: To describe the proper procedure to follow when injecting adjuvants into rabbits for the production of polyclonal antibodies. POLICY: The improper

More information

Titanium Dioxide Raspberry Solar Cell 2012, 2011, 2005 by David A Katz. All rights reserved

Titanium Dioxide Raspberry Solar Cell 2012, 2011, 2005 by David A Katz. All rights reserved Titanium Dioxide Raspberry Solar Cell 2012, 2011, 2005 by David A Katz. All rights reserved Greg Smestad (http://www.solideas.com/solrcell/cellkit.html) developed this experiment. See the Nanocrystalline

More information

Revised: June 6, 2007

Revised: June 6, 2007 Isotropic Silicon Etching using HF/Nitric/Acetic Acid (HNA) Standard Operating Procedure Faculty Supervisor: Prof. Robert White, Mechanical Engineering (x72210) Safety Office: Peter Nowak x73246 (Just

More information

Minimizing Occupational Exposure to Hazardous Chemicals in Animal Protocols

Minimizing Occupational Exposure to Hazardous Chemicals in Animal Protocols Environmental Health and Safety Minimizing Occupational Exposure to Hazardous Chemicals in Animal Protocols Chemicals that are highly toxic or corrosive, known or suspected carcinogens, reproductive hazards,

More information

Occupational Hazards for Health Care Workers. Photos from Occupational Safety and Health Administration, USA

Occupational Hazards for Health Care Workers. Photos from Occupational Safety and Health Administration, USA Occupational Hazards for Health Care Workers Photos from Occupational Safety and Health Administration, USA Who is a Health Care Worker? Nurse Doctor Aide, orderly Pharmacist Laundry worker Housekeeper

More information

Sanitizers for Food Plants

Sanitizers for Food Plants s for Food Plants The selection of a sanitizer depends on the type of equipment to be sanitized, the hardness of the water, the application equipment available, the effectiveness of the sanitizer under

More information

Chlorine, Total. DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials. Test preparation. Instrument-specific information

Chlorine, Total. DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials. Test preparation. Instrument-specific information Chlorine, Total DOC316.53.01028 DPD Method 1 Method 10101 0.09 to 5.00 mg/l Cl 2 Test 'N Tube Vials Scope and application: For testing higher levels of total (free plus combined) chlorine in drinking water,

More information

UNCG Safety and Health Policy and Procedure Manual Section 0260 Page 1 of 14 Date: 1/08 Revised: 12/09

UNCG Safety and Health Policy and Procedure Manual Section 0260 Page 1 of 14 Date: 1/08 Revised: 12/09 Section 0260 Page 1 of 14 I. POLICY All persons at the University will handle Regulated Medical Waste and other biohazard waste so as to minimize hazardous exposure to themselves, other persons, and the

More information

CONTROL: For staining fungus; use a known positive such as those used for the GMS. Use skin, aorta or normal liver for positive PAS staining.

CONTROL: For staining fungus; use a known positive such as those used for the GMS. Use skin, aorta or normal liver for positive PAS staining. SURGICAL PATHOLOGY - HISTOLOGY Date: STAINING MANUAL - CARBOHYDRATES Page: 1 of 3 PAS - McMANNUS' PERIODIC ACID SCHIFF'S - GLYCOGEN PURPOSE: Glycogen is present in skin, liver, parathyroid glands and skeletal

More information

Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP)

Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP) Particularly Hazardous Substances (PHS) Standard Operating Procedure (SOP) Formaldehyde, formalin, paraformaldehyde solutions, and paraformaldehyde solids Principal Investigator: Room & Building #: Department:

More information

Ingredient % content Hazard data Cross linked Sodium Polyacrylite 70-90 CAS No:9003-04-7 Non chemical fillers 20-30 Zeolite 1-10

Ingredient % content Hazard data Cross linked Sodium Polyacrylite 70-90 CAS No:9003-04-7 Non chemical fillers 20-30 Zeolite 1-10 Body Spill Granules Composition Ingredient % content Hazard data Cross linked Sodium Polyacrylite 70-90 CAS No:9003-04-7 Non chemical fillers 20-30 Zeolite 1-10 Hazards Identification Eye contact: May

More information

Standard Operating Procedure

Standard Operating Procedure Standard Operating Procedure FLAMMABLE LIQUIDS This standard operating procedure (SOP) is intended to provide general guidance on how to safely work with flammable liquids. This SOP is generic in nature

More information

DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included.

DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included. DYES AND DYEING 2003 by David A. Katz. All rights reserved. Permission for classroom use provided original copyright is included. Dyeing of textiles has been practiced for thousands of years with the first

More information

Basic Reptile Rehabilitation

Basic Reptile Rehabilitation Basic Reptile Rehabilitation Identification Know your local species Visit and work with nature centers Hike/get out in the field Identification Know your local species Male Three toed box turtle Identification

More information

Material Safety Data Sheet. RM 110 Scale Inhibitor Liquid Mix Kit Concentrate

Material Safety Data Sheet. RM 110 Scale Inhibitor Liquid Mix Kit Concentrate of 5 PRODUCT AND COMPANY IDENTIFICATION Manufacturer Kärcher North America, Inc. 325 S Price Road Chandler, AZ 85224 Phone: Fax: Email: Web: 360-833-600 360-833-9200 info@karcherna.com www.karcher.com

More information

MATERIAL SAFETY DATA SHEET Description: SAF DRAIN Revision Number: 00

MATERIAL SAFETY DATA SHEET Description: SAF DRAIN Revision Number: 00 GRANULAR, ALKALINE DRAIN CLEANER GENERAL SAF-DRAIN is a powerful alkaline drain cleaner designed so that the product reaches the problem area before the reaction takes place. It effectively cleans organic

More information

Agricultural and Veterinary Chemicals Code (Listed Chemical Product Home Swimming Pool and Spa Products) Standard 2014

Agricultural and Veterinary Chemicals Code (Listed Chemical Product Home Swimming Pool and Spa Products) Standard 2014 Agricultural and Veterinary Chemicals Code (Listed Chemical Product Home Swimming Pool and Spa Products) Standard 2014 I, Kareena Arthy, Chief Executive Officer of the Australian Pesticides and Veterinary

More information

Guidelines for Collection of Blood from Laboratory Animals

Guidelines for Collection of Blood from Laboratory Animals Guidelines for Collection of Blood from Laboratory Animals These guidelines have been developed to introduce investigative staff to procedures recommended for blood collection in laboratory animals. This

More information

Cleaning and Disinfecting

Cleaning and Disinfecting Handout #5.1 It is inevitable that germs will spread to surfaces and objects after being soiled with blood or bodily fluids such as stool, urine, vomit, mucus, saliva, human milk, etc. To prevent the spread

More information

MATERIAL SAFETY DATA SHEET Complies with ANSI Z400.1 Format SECTION 1: PRODUCT IDENTIFICATION

MATERIAL SAFETY DATA SHEET Complies with ANSI Z400.1 Format SECTION 1: PRODUCT IDENTIFICATION Staples the Office Superstore, LLC. MSDS 500 Staples Drive Hazard Rating HMIS NFPA Framingham, MA 01702 USA Health Business Phone: 1.800.322.0912 Flammability 24-HR MEDICAL AND DOT EMERGENCIES: 1.800.322.0912

More information

MATERIAL SAFETY DATA SHEET PRODUCT NAME:

MATERIAL SAFETY DATA SHEET PRODUCT NAME: PRODUCT AND COMPANY INFORMATION SECTION 1 Company Identification Name: Becton Dickinson Medical Systems Address: 9450 South State Street Sandy, UT 84070 FOR CHEMICAL EMERGENCY, SPILL, LEAK, FIRE, EXPOSURE,

More information

Sterilization, Disinfection and Biosafety. Department of Microbiology College of Medicine University of Baghdad

Sterilization, Disinfection and Biosafety. Department of Microbiology College of Medicine University of Baghdad Sterilization, Disinfection and Biosafety Department of Microbiology College of Medicine University of Baghdad Learning Objectives Define Sterilization and disinfection Identify the different types or

More information

Subsection 4.1.6 Cleaning And Sanitizing for Licensed Group Child Care Homes, Licensed Child Care Centers and License-Exempt Child Care Facilities

Subsection 4.1.6 Cleaning And Sanitizing for Licensed Group Child Care Homes, Licensed Child Care Centers and License-Exempt Child Care Facilities Page 1 of 6 CLEANING AND SANITIZING All items requiring sanitizing shall be washed, rinsed and sanitized with approved agents, methods and concentrations. [19 CSR 30-60.090 (1) (B), (5) (A), 19 CSR 30-62.082

More information

Lab Exercise 3: Media, incubation, and aseptic technique

Lab Exercise 3: Media, incubation, and aseptic technique Lab Exercise 3: Media, incubation, and aseptic technique Objectives 1. Compare the different types of media. 2. Describe the different formats of media, plate, tube etc. 3. Explain how to sterilize it,

More information

Cleaning. By the end of this chapter, you will be able to: Introduction. Definitions. Chapter 9

Cleaning. By the end of this chapter, you will be able to: Introduction. Definitions. Chapter 9 Chapter 9 By the end of this chapter, you will be able to: l Define the terms cleaning, decontamination, disinfection l List the factors that affect disinfection l Describe the aims of a cleaning schedule

More information

Republic of Namibia Ministry of Health and Social Services STANDARD GUIDELINES ON INFECTION PREVENTION CONTROL IN DENTAL SURGERY

Republic of Namibia Ministry of Health and Social Services STANDARD GUIDELINES ON INFECTION PREVENTION CONTROL IN DENTAL SURGERY Republic of Namibia Ministry of Health and Social Services STANDARD GUIDELINES ON INFECTION PREVENTION CONTROL IN DENTAL SURGERY November 2012 Republic of Namibia Ministry of Health and Social Services

More information

GUIDELINES FOR THE MANGAGEMENT OF BIO- MEDICAL WASTE AT THE SCHOOL OF LIFE SCIENCES

GUIDELINES FOR THE MANGAGEMENT OF BIO- MEDICAL WASTE AT THE SCHOOL OF LIFE SCIENCES Basic Safety Rules SV vademecum Safety information Version 1.2 17.04.2013 GUIDELINES FOR THE MANGAGEMENT OF BIO- MEDICAL WASTE AT THE SCHOOL OF LIFE SCIENCES Table of contents 1. General Information...

More information