MASTERS OF PHILOSOPHY (M. PHIL.) BIOCHEMISTRY DETAILED SYLLABUS SESSION
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1 MASTERS OF PHILOSOPHY (M. PHIL.) BIOCHEMISTRY DETAILED SYLLABUS SESSION
2 UNIT - I PAPER I RESEARCH METHODOLOGY THEORY AND TECHNIQUES Research: Definition, Importance and Meaning of research, Characteristics of research, Types of Research, Steps in research, Identification, Selection and formulation of research problem, Research questions Research design Formulation of Hypo Dissertation, Review of Literature. UNIT II Sampling techniques: Sampling theory, types of sampling Steps in sampling Sampling and Non-sampling error Sample size Advantages and limitations of sampling. Collection of Data: Primary Data Meaning Data Collection methods Secondary data Meaning Relevances, limitations and cautions. UNIT III Statistics in Research Measure of Central tendency, Dispersion, Skewness and Kurtosis in research, Hypo Dissertation, Fundamentals of Hypo Dissertation testing, Standard Error, Point and Interval estimates, Important Non-Parametric tests: Sign, Run, Kruskal, Wallis tests and Mann, Whitney test. UNIT IV Para metric tests: Testing of significance, mean, Proportion, Variance and Correlation, testing for Significance of difference between means, proportions, variances and correlation co-efficient. Chi-square tests, ANOVA, One-way and Two-way. UNIT V Research Report: Types of reports, contents, styles of reporting, Steps in drafting reports, editing the final draft, evaluating the final draft. 1. Statistical Methods - S.P. Gupta Reference Books: 2. Research Methodology Methods and Techniques - C.R. Kothari 3. Statistics (Theory and Practice) - B.N. Gupta 4. Research Methodology Methods and Statistical Techniques - Santosh Gupta
3 UNIT I PAPER-II PRINCIPLE OF BIOCHEMISTRY Living Organism: The molecular logic of Living Organism, Water- properties, Electrolytic dissociation into cations and anions Henderson Hasselbalch equation, Proteins General structural design. Aminoacids and peptides covalent structure, three dimensional structure and subunit structure. Fibrous proteins and Globular proteins. Structure and function of Haemoglobin and Myoglobin, Carbohydrates structure and biological function, Enzymes classification of enzymes. Specificity of enzyme action. Allosteric enzymes, enzyme inhibition and regulation of enzyme activity. Enzyme kinetics. Factors affecting enzyme activity. Vitamins as Coeznymes. Isoenzymes (LDH), Lipids Saturated and unsaturated fatty acids. Triglycerides, phospholipids, glycolipids. Sterols prostaglandins. UNIT II Cellular Metabolism and Metabolic Cycles & Energy Production: Thermodynamics of cellular reactions. Redox potential, Energy rich compounds and their biological significance,biological oxidation, Mitochondrial Electron transport, Oxidative phosphorylation and ATP synthesis, Chemiosmotic theory. Metabolic Cycles and Energy Production: Glycolysis A central pathway of glucose catabolism, TCA cycle and its significance, Hexosc Mono-phosphate shunt, Fatty acid oxidation, ketone bodies, ketosis and metabolism of ketone bodies, Glycogenolysis, Glycerol metabolism UNIT III Glycogenesis and Protein Metabolisms: Glycogenesis, Gluconeogenesis, Regulation of carbohydrate metabolism, Biosynthesis of fatty acids, Interrlationship between carbohydrate and lipid metabolism, Cholesterol synthesis and degradation. Protein Metabolisms: Deamination, transmination, decarhoxylation, Urea cycle UNIT IV General characteristics of hormones: Chemistry and function of hormones, hormone receptors and hormone action, Hormonal control of cellular metabolism.
4 UNIT V Nucleic acids structure and function: Purine and pyramidine derivatives, Nucleosides, nucleotides and internucleotide linkages, Chemical nature of DNA and RNA, Biological functions of nucleic acids References Books: 1. Biochemistry - L.Stryer 2. Principles of Biochemistry - A.L.Lehninger 3. Concepts in Biochemistry - Conn & Stumpf 4. Principles of Biochemistry - E.L.Smith, R.L.Hill et al Vol. I & II (7 th Edn.) 5. Harper s Biochemistry - Murray, R.K., Granner, D.K., Mayes, P.A. & Rodwell, V.W. PAPER-III INDUSTRIAL BIOTECHNOLOGY UNIT I Introduction To Industrial Bioprocess: A historical overview of industrial fermentation process traditional and modern biotechnology. A brief survey of organisms, processes, products relating to modern biotechnology. Process flow sheeting block diagrams, pictorial representation. UNIT II Production Of Primary Metabolites: A brief outline of processes for the production of some commercially important organic acids (e.g. citric acid, lactic acid, acetic acid etc.,); amino acids (glutamic acid, phenyalanine, aspartic acid etc.,) and alcohols (ethanol, butanol etc.,) UNIT III
5 Production Of Secondary Metabolites: Study of production processes for various classes of secondary metabolites: antibiotics: beta-lactams (penicillin, cephalosporin etc.), aminoglycosides (streptomycin etc.,) macrolides (erythromycin), vitamins and steroids. UNIT IV Production Of Enzymes And Other Bioproducts: Production of industrial enzymes such as proteases, amylases, lipases, cellulases etc., Production of biopesticides, biofertilisers, biopreservatives (Nisin), cheese, biopolymers (xanthan gum, PHB etc.,), single cell protein. UNIT V Production Modern Biotechnology Products: Production of recombinant proteins having therapeutic and diagnostic applications, production of vaccines. Production of monoclonal antibodies. Products of plant and animal cell culture References: 1. Wulf Cruger and Anneliese Crueger, Biotechnology: A Textbook of Industrial Microbiology, Panima Publishing Corporation. 2. Murrey Moo & Young, Comprehensive Biotechnology, Pergamon. Casida Jr, L.E., Industrial Microbiology, New Age International (P) Ltd. 3. Presscott, Dunn, Industrial Microbiology, Agrobios (India). PAPER-IV DISSERTATION
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