FTA Bacterial DNA Whatman

Transcription

1 FTA Bacterial DNA Whatman Raccolta, trasferimento, stoccaggio e purificazione di DNA batterico La tecnologia FTA della Whatman offre un valido e semplice metodo per la preparazione di campioni per analisi genetiche da batteri. Occorre semplicemente applicare campioni di colture o campioni clinici sulla matrice FTA il DNA viene catturato e stabilizzato all istante, consentendone lo stoccaggio a temperatura ambiente, da analizzare al momento più opportuno. Gli agenti patogeni vengono inattivati all istante. Trasporto sicuro e purificazione in 30 minuti rendono la tecnologia FTA un indispensabile strumento di ricerca. Caratteristiche Principali Indicato per un ampia varietà di batteri Il DNA genomico pronto per la PCR può essere isolato rapidamente da una varietà di batteri gram-negativi e grampositivi, batteri spore-formanti e acido-resistenti senza alcun o minimo pretrattamento. Raccolta semplice Depositare colture o campioni clinici direttamente sulla matrice FTA (FTA Card Indicatrice). Perfetto per il vostro metodo FTA funziona, qualsiasi il metodo scelto per l identificazione di batteri: campioni da colture pure di batteri o campioni clinici. Temperatura ambiente Gli acidi nucleici vengono automaticamente stabilizzati senza la necessità di refrigerazione. Applicazioni Applicazioni diagnostiche Analisi biologiche Analisi di alimenti Analisi ambientali Rapida purificazione Il DNA viene purificato sulla FTA Card in tre semplici steps, in un singolo tubo, a temperatura ambiente. Il DNA rimane immobilizzato sulla matrice ed è subito pronto per la PCR. Se è necessario avere DNA in soluzione si può usare Whatman GenSpin. Manipolazione e trasferimento sicuri FTA inattiva gli agenti patogeni potenzialmente nocivi. Automatizzazione L utilizzo di sistemi automatici accelera le fasi di preparazione di diversi dischi prelevati contemporaneamente, ottimizza le risorse disponibili e standardizza la purificazione di DNA. I dischi prelevati dalla FTA Card possono essere sottoposti alle fasi di lavaggio e preparazione per la PCR su una diversi tipi di strumenti presenti in commercio (liquid handling robot). Monitoraggio acqua/aria Identificazioni molecolari Applicazioni di ricerca Tre semplici passaggi operativi per ottenere un DNA puro Dispensare il campione Purificare Prelevare un piccolo disco (punch) dal Campione

2 PROTOCOLLO APPLICATIVO DEL FTA BACTERICAL DNA Dispensare il campione Dispensare il campione sulla FTA Card. Lasciarlo asciugare completamente. Se si utilizza una Card Indicatrice, l area coperta dal campione cambierà colore da rosa a bianco indicando la posizione del campione stesso. Prelevare un piccolo disco Prelevare (mediante perforazione) un piccolo disco dal campione depositato sulla FTA Card. Purificazione con Reagente di Purificazione FTA (Purification Reagent FTA) Mettere il disco di campione in un tubo per PCR ed effettuare tre lavaggi con il Reagente di Purificazione FTA. Eliminare la soluzione utilizzata dopo ciascun lavaggio. Lavaggio con soluzione tampone TE-1 Effettuare due lavaggi con la soluzione tampone TE-1 (10mM Tris, 0,1 mm EDTA, ph 8,0). Eliminare la soluzione usata dopo ciascun lavaggio. Essiccazione Lasciar asciugare il disco nel tubo PCR PCR Aggiungere la master mix per la PCR direttamente al disco e iniziare la reazione di amplificazione. Qualità Whatman Whatman, azienda leader nelle tecnologie per la separazione, è nota nellà comunità scientifica per prodotti e soluzioni innovative contraddistinte da un elevata qualità. Il nostro istinto per la semplificazione accelera la velocità di fare nuove scoperte, riduce i costi e risparmia tempo. Inoltre, per accentuare ulteriormente l impegno nel soddisfare le specifiche esigenze dei nostri clienti, Whatman è organizzata in quattro aree distinte: LabScience, BioScience, MedTech Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono disponibili collegandovi al sito FTA, GenSpin e Whatman sono marchi registrati della Whatman Group. INFORMAZIONI PER L ORDINE DAL CATALOGO WHATMAN No. Cat. Descrizione Quantità per Confezione WB FTA Starter Pack 1 WB FTA Reagente di Purificazione 500mL WB FTA Classic Card (non-indicatrice) 100 WB FTA Classic Card (indicatrice) 100 WB FTA Mini Card (non-indicatrice) 100 WB FTA Mini Card (indicatrice) 100 WB FTA Micro Card (non-indicatrice) 100 WB FTA Micro Card (indicatrice) 100 WB Harris Micro Perforatore 1.2mm 1 WB Harris Micro Perforatore 1.2mm Tip 1 WB Harris Uni-Core 1.25mm Perforatore 4 WB Busta Multi-Barrier (grande) 500 WB Busta Multi-Barrier (piccola) 500 WB mm Plunger di Ricambio 1 WB Essiccante (1g) 1000 WB Busta Postale per FTA Card 50 WB Tagliere di Ricambio 1 WB GenSpin Kit di Purificazione DNA 50 Purificazioni Purificazione del DNA in meno di 25 minuti! Il GenSpin Kit di Purificazione del DNA della Whatman rappresenta un metodo estremamente semplice per purificare il DNA a singolo filamento da campioni di sangue e cellule in coltura. Il campione è subito pronto per la PCR a partire anche da piccoli volumi del campione di partenza. North America Europe Japan Asia Pacific Whatman Inc 9 Bridewell Place, Clifton, NJ Technical Support: Customer Service: Outside US: Fax: info@whatman.com Whatman International Ltd Springfield Mill James Whatman Way Maidstone Kent ME14 2LE, UK Tel: +44 (0) Fax: +44 (0) information@whatman.com Whatman Japan KK Daiwa Ueno Building 1F 6-10 Ueno 5-chome, Taito-ku Tokyo , Japan Tel: +81 (0) Fax: +81 (0) japaninfo@whatman.com Whatman Asia Pacific Pte Ltd 171 Chin Swee Road #08-01 San Centre Singapore Tel: Fax: wap@whatman.com GenSpin TM Kit di Purificazione DNA Whatman Leaders in Separations Technology Cat No. S

3 FTA Cards and Indicating FTA Cards WHATMAN CATALOGUE ORDERING INFORMATION Catalogue Number WB WB WB WB WB WB WB WB WB FTA Classic Card Indicating FTA Classic Card* FTA Mini Card Indicating FTA Mini Card* FTA Micro Card Indicating FTA Micro Card* FTA Gene Card** PlantSaver Card CloneSaver Card * Indicating FTA Cards change colour from pink to white when sample is applied and are recommended for use with clear samples. **FTA Gene Cards are compatible with automated liquid sampling systems when used with FTA Gene Card Trays. FTA Reagent, Accessories and Kits WHATMAN CATALOGUE ORDERING INFORMATION Catalogue Number WB WB WB WB WB WB WB WB WB WB WB WB Description Cards/ Pack Description Sample Areas/ Card FTA Card Mailer FTA Gene Card Tray FTA Starter Pack FTA Kit FTA Purification Reagent Sterile Foam Tipped Applicator Swabs Desiccant (1gm) Multi-Barrier Pouch, Small (for Mini, Micro and Gene Cards) Multi-Barrier Pouch, Large (for Classic Cards) CloneSaver Resealable Multi-Barrier Pouch CloneSaver Starter Kit Harris Micro Punch 1.2mm (with Mat) Maximium Volume/ Sample Area (µl) N/A 5 Maximium Total Volume/Card (µl) N/A 480 Qty per Pack mL Whatman Quality Whatman is a global leader in separations technology and is known in the scientific community for providing innovative Life Science products and solutions. Our instinct for simplification accelerates the rate of discovery, reduces costs and saves time. In order to focus on the unique needs of our customers, Whatman is organised into four business development units: Analytical Chemistry, Diagnostics, Genomics & Proteomics and Medical Devices. For more information, visit CloneSaver, FTA, PlantSaver and Whatman are registered trademarks of the Whatman Group. North America Europe Japan Asia Pacific Whatman Inc. 200 Park Avenue Florham Park, NJ USA Technical Support: Customer Service: info@whatman.com Whatman International Ltd Springfield Mill James Whatman Way, Maidstone Kent ME14 2LE UK Tel: + 44 (0) Fax: + 44 (0) information@whatman.com Whatman Japan KK Daiwa Ueno Building 1F 6-10 Ueno 5-chome, Taito-ku Tokyo , Japan Tel: +81 (0) Fax: +81 (0) japaninfo@whatman.com Whatman Asia Pacific Pte Ltd 171 Chin Swee Road #08-01 San Centre Singapore Tel: Fax: wap@whatman.com WB Harris Uni-Core Disposable 1.25mm Punch (with Mat) 4 WB Harris Micro Punch 2.0mm (with Mat) 1 WB WB Harris Uni-Core Disposable 2.0mm Punch (with Mat) Replacement Cutting Mat 4 1 Leaders in Separations Technology WB Replacement Tip 1.2mm 1 WB Replacement Tip 2.0mm 1 Cat No. S

4 Whatman FTA Collect, archive, transport and purify nucleic acids all at room temperature Whether you re in a laboratory or deep in a rain forest, Whatman FTA provides a remarkably easy way to collect and isolate nucleic acid samples for analysis. Simply apply virtually any type of biological sample to the FTA matrix, and the nucleic acids are instantly captured and stabilised. Pathogens are inactivated, making samples safe to handle and ship. Store samples, including clones, at room temperature and analyse whenever you re ready. Try FTA, and you ll soon find it s an indispensable part of your nucleic acids toolbox. Features and Benefits Three easy steps to pure nucleic acids Simple collection Protection of nucleic acids from degradation at room temperature allows for convenient collection in the laboratory or the field. Room temperature storage Nucleic acids are automatically stabilised without the need for refrigeration. Pathogen inactivation Cells are automatically lysed on contact with the FTA matrix. Pathogens become inactivated, making samples safe to handle and ship via standard mail. Fast purification Nucleic acids are purified on the FTA Card in three simple steps, all in a single tube at room temperature. DNA remains immobilised on the matrix and is ready for PCR or other amplification techniques. Automatable Automation speeds the handling of multiple FTA punches and standardises DNA purification. Punches can be easily washed and prepared for PCR on a variety of liquid handling instruments. Purify Spot Punch Applications Blood, plant, insect, viral and bacterial analysis Genetic identification Ideal for clones Diagnostic and clinical applications Biosafety, food safety and environmental analysis HLA typing Animal breeding studies Molecular identification

5 FTA A Highly Flexible Technology used Widely in a Range of Industries FTA technology has been embraced by a wide range of industries across the globe. Pharmaceutical companies use FTA to collect and archive human DNA samples for clinical drug trials. Law enforcement agencies use FTA to collect and archive DNA samples from convicted offenders. Nature conservationists use FTA to collect bird DNA from jet engines to determine the flight patterns of specific species. Scientists hunting for new plant species use FTA in the field to collect and safely transport samples. Governmental agencies use FTA to sample food products while farmers use FTA to track diseases within multiple herd generations. While the range of applications is large, they all share a common element: simplicity. Whatman FTA helps scientists speed their research and achieve their goals. Use with virtually any cell type The following is a partial list of the cell types that can be applied to FTA Cards: Blood Cultured cells Buccal cells Plant tissue Bacteria Plasmids Micro-organisms Solid tissue Viral particles M13 plaques FTA Cards are available in either white or pink (Indicating) formats. White FTA Cards are recommended for blood samples, plant tissues and other easily identified samples. Indicating FTA Cards are pink and turn white upon sample addition. Indicating FTA Cards are recommended for buccal cells, cultured cells and other clear samples. Store nucleic acids at room temperature for years Genomic DNA stored on FTA Cards at room temperature for more than 14 years has been successfully amplified by PCR. No other product can make that claim. FTA Cards offer a compact room temperature storage system that reduces the need for precious freezer space, improves sample accessibility and reduces storage costs. Captured nucleic acid is ready for downstream applications in less than 30 minutes Captured nucleic acid is ready for purification when you are. Just take a sample disk from the FTA Card, wash with FTA Purification Reagent and rinse with TE -1 buffer. The washed disk is ready to use in applications such as PCR, RFLP analysis and RT-PCR. FTA DNA PURIFICATION PROTOCOL Sample Application Apply specimen and allow to dry completely. Disk Removal Punch a disk out of the sample area on the FTA Card. FTA Purification Reagent Washes Place the disk in a PCR tube and wash three times with FTA Purification Reagent. Discard used reagent after each wash. TE -1 Rinses Wash twice with TE -1 buffer (10 mm Tris, 0.1 mm EDTA, ph 8.0) and discard used buffer after each wash. Drying Step Dry disk in PCR tube. Direct to PCR Add PCR master mix directly to the disk and amplify.

6 FTA Cards, Reagent, Accessories and Kits FTA Cards FTA Cards are available in 1, 2, 3 and 4 part configurations. Custom configurations are available upon request. FTA Classic Card Four sample areas for storage of up to 500µL whole blood or 100µL plant homogenate per card. Convenient for multiple applications of the same specimen or collection of multiple animal or plant samples. Also available in Indicating (pink) FTA format. FTA Mini Card Two sample areas for storage of up to 250µL whole blood or 50µL plant homogenate per card. Convenient for protocols that require different locations for testing and archiving samples. Also available in Indicating (pink) FTA format. FTA Micro Card One sample area for storage of up to 125µL whole blood or 25µL plant homogenate per card. Recommended when only one sample is needed. Also available in Indicating (pink) FTA format. FTA Gene Card Three sample areas in a card frame for storage of up to 225µL whole blood or 30µL plant homogenate per card. Can be run in most automatic dispensing/pipetting systems when used with the FTA Gene Card Tray. CloneSaver Card Designed for the collection, storage and purification of plasmid and BAC DNA from bacterial clones. DNA is stable at room temperature for at least 5 years (real-time data). Available in a 96 well format for high throughput applications. PlantSaver FTA Card Plant friendly FTA Card, in a Classic Card format. Features a laminated flap that allows you to vigorously pound the plant sample into the FTA matrix without damaging the FTA Card. FTA Reagent, Accessories and Kits FTA Purification Reagent Removes heme, PCR inhibitors and other potential contaminants to ensure superior quality DNA for downstream analysis. FTA Gene Card Tray Holds two FTA Gene Cards for use in automatic liquid handling systems. FTA Kit Includes a 25-card supply of FTA Micro Cards; two vials of purification reagent (25mL); two Harris Uni-Core Punches; a cutting mat and instructions. FTA Starter Pack Provides a sample of FTA products, including one FTA Classic Card; one FTA Mini Card; one FTA Micro Card; one Indicating FTA Mini Card and one Indicating FTA Micro Card. Pack also includes two foam-tipped applicator swabs; one multi-barrier pouch and desiccant; one vial of purification reagent (25mL); two Harris Uni-Core Punches; a cutting mat and instructions. Sterile Foam Tipped Applicator Easy-to-use applicator for the non-invasive collection and transfer of buccal cells to FTA Cards. Harris Micro Punches, Disposable Uni-Core Punches and Cutting Mat For the precise sample disk removal from FTA Cards. The 1.2mm punches are recommended for use with whole blood and samples with high DNA content. The 2.0mm punches are recommended for use with buccal cells, plasmids and samples with lower DNA content. Multi-Barrier Pouches For transporting or storing FTA Cards. Protects cards from environmental contamination. Tamper-evident seal maintains sample security for forensics samples. A resealable pouch is also available when multiple access to FTA Cards is needed. FTA Card Mailer A rigid protective card mailer for transporting FTA Cards without biohazard labelling. Storage Desiccant Packets Ensure that FTA Cards remain dry during transport or storage. Contains indicator that changes colour to verify moisture absorption. CloneSaver Starter Kit Includes two CloneSaver Cards; two Harris Uni-Core Punches (2mm); a cutting mat and instructions.

7 FTA Mouse Tail DNA Whatman Raccolta, trasferimento, stoccaggio e purificazione del DNA da code di topo FTA della Whatman offre un valido e semplice metodo per la preparazione di campioni per analisi di DNA da code di topo. Occorre, infatti, semplicemente applicare un campione di sangue o di tessuto dalla coda del topo alla matrice FTA. Il DNA viene catturato e stabilizzato all istante, consentendone lo stoccaggio per un periodo indefinito a temperatura ambiente, da analizzare in qualsiasi momento. Trasporto sicuro e purificazione in 30 minuti rendono la tecnologia FTA un indispensabile strumento di ricerca. Caratteristiche Principali Metodo rapido e versatile di raccolta I campioni sono depositati direttamente sulla matrice FTA. Il DNA è purificato sulla FTA Card in tre steps semplici, in un singolo tubo, a temperatura ambiente. Non sono necessarie sostanze chimiche tossiche. Il DNA rimane fissato sulla matrice, pronto per la PCR. Adatto a qualsiasi metodo Le FTA Cards si adattano alle diverse tipologie di campione : preparazione di campioni di sangue o di campioni di tessuto di coda di topo previo pretrattamento mediante digestione enzimatica. E sufficiente applicare direttamente i campioni sulla matrice FTA e non è necessaria alcuna ulteriore purificazione né con sostanze tossiche (metodi quali fenolo/cloroformio) né con altri metodi che possono richiedere lunghe incubazioni. Stoccaggio a temperatura ambiente e trasferimento sicuri Gli acidi nucleici vengono automaticamente stabilizzati senza la necessità di congelarli. FTA inattiva gli agenti patogeni potenzialmente nocivi rendendo i campioni sicuri per la loro manipolazione in laboratorio. Inviare i campioni a colleghi o al laboratorio centrale è veramente facile con FTA Cards. Occorre solo inviarli per posta! Automatizzabile L utilizzo di sistemi automatici accelera le fasi di preparazione di diversi dischi prelevati contemporaneamente, ottimizza le risorse disponibili e standardizza la purificazione di DNA. I dischi prelevati dalla FTA Card possono essere sottoposti alle fasi di lavaggio e preparazione per la PCR su una diversi tipi di strumenti presenti in commercio (liquid handling robot). Tre semplici passaggi operativi per ottenere un DNA puro Dispensare il campione Purificare Prelevare un piccolo disco (punch) dal Campione Applicazioni Screening di topi transgenici Identificazione genetica Studi di allevamento Applicazioni diagnostiche PCR Analisi SNP Whole genome amplification

8 PROTOCOLLO APPLICATIVO DEL FTA MOUSE TAIL DNA Dispensare il campione Tagliare approssimativamente 1,5cm di coda del topo, stringere gentilmente perchè il sangue venga alla superficie del taglio, appoggiare leggermente la FTA Card sul sangue. Oppure applicare la raccolta direttamente sull FTA Card. Lasciare asciugare completamente. Prelevare un piccolo disco Prelevare (mediante perforazione) un piccolo disco dal campione depositato sulla FTA Card. Purificazione con Reagente di Purificazione FTA (Purification Reagent FTA) Mettere il disco di campione in un tubo per PCR ed effettuare tre lavaggi con il Reagente di Purificazione FTA. Eliminare la soluzione utilizzata dopo ciascun lavaggio. Lavaggio con soluzione tampone TE-1 Effettuare due lavaggi con la soluzione tampone TE-1 (10mM Tris, 0,1 mm EDTA, ph 8,0). Eliminare la soluzione usata dopo ciascun lavaggio. Essiccazione Lasciar asciugare il disco nel tubo PCR. PCR Aggiungere la master mix per la PCR direttamente al disco e iniziare la reazione di amplificazione. Qualità Whatman Whatman, azienda leader nelle tecnologie per la separazione, è nota nellà comunità scientifica per prodotti e soluzioni innovative contraddistinte da un elevata qualità. Il nostro istinto per la semplificazione accelera la velocità di fare nuove scoperte, riduce i costi e risparmia tempo. Inoltre, per accentuare ulteriormente l impegno nel soddisfare le specifiche esigenze dei nostri clienti, Whatman è organizzata in quattro aree distinte: LabScience, BioScience, MedTech Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono disponibili collegandovi al sito FTA, GenSpin e Whatman sono marchi registrati della Whatman Group. INFORMAZIONI PER L ORDINE DAL CATALOGO WHATMAN No. Cat. Descrizione Quantità per Confezione WB FTA Starter Pack 1 WB FTA Reagente di Purificazione 500mL WB FTA Classic Card (non-indicatrice) 100 WB FTA Mini Card (non-indicatrice) 100 WB FTA Micro Card (non-indicatrice) 100 WB FTA Gene Card 100 WB Harris Micro Perforatore 2.0mm 1 WB Harris Micro Perforatore 2.0mm Tip 1 WB Harris Uni-Core 2.0mm Perforatore 4 WB Busta Multi-Barrier (grande) 500 WB Busta Multi-Barrier (piccola) 500 WB FTA Gene Card/Busta/Essiccante 1000 WB FTA Classic Card/Busta/Essiccante 1000 WB mm Plunger di Ricambio 1 WB Essiccante (1g) 1000 WB Busta Postale per FTA Card 50 WB Tagliere di Ricambio 1 WB Supporto per FTA Gene Card 20 WB GenSpin Kit di Purificazione DNA 50 Purificazioni Purificazione del DNA in meno di 25 minuti! Il GenSpin Kit di Purificazione del DNA della Whatman rappresenta un metodo estremamente semplice per purificare il DNA a singolo filamento da campioni di sangue e cellule in coltura. Il campione è subito pronto per la PCR a partire anche da piccoli volumi del campione di partenza. North America Europe Japan Asia Pacific Whatman Inc 9 Bridewell Place, Clifton, NJ Technical Support: Customer Service: Outside US: Fax: info@whatman.com Whatman International Ltd Springfield Mill James Whatman Way Maidstone Kent ME14 2LE, UK Tel: +44 (0) Fax: +44 (0) information@whatman.com Whatman Japan KK Daiwa Ueno Building 1F 6-10 Ueno 5-chome, Taito-ku Tokyo , Japan Tel: +81 (0) Fax: +81 (0) japaninfo@whatman.com Whatman Asia Pacific Pte Ltd 171 Chin Swee Road #08-01 San Centre Singapore Tel: Fax: wap@whatman.com GenSpin TM Kit di Purificazione DNA Whatman Leaders in Separations Technology Cat No. S

9 FTA Plant DNA Whatman Raccolta, trasferimento e purificazione del DNA di Piante In laboratorio o in mezzo ad una foresta, FTA della Whatman offre un valido e semplice metodo per la preparazione di campioni per analisi di DNA di piante. Occorre, infatti, semplicemente applicare sulla matrice FTA il campione vegetale tal quale o dopo averne ottenuto un omogenato. Il DNA viene catturato e stabilizzato all istante, consentendone lo stoccaggio per un periodo indefinito a temperatura ambiente, da analizzare in qualsiasi momento. Trasporto sicuro e purificazione in 30 minuti rendono la tecnologia FTA un indispensabile strumento di ricerca. Caratteristiche Principali Raccolta semplice di campioni sul campo La stabilità degli acidi nucleici a temperatura ambiente e la protezione dalla degradazione rendono la tecnologia FTA uno strumento ideale per la raccolta di campioni sul campo. Meno campione E possible utilizzare anche solo le foglie più giovani, riducendo il tempo di crescita necessario e accelerando la ricerca. Stoccaggio a temperatura ambiente e trasferimento sicuri Consente di raccogliere ovunque il DNA dalle piante, di trasportarlo al laboratorio e purificarlo solo quando necessario. Purificazione rapida Il DNA viene purificato sulla FTA Card in tre semplici steps, in un singolo tubo, a temperatura ambiente. Non sono necessarie sostanze chimiche tossiche, inoltre rimanendo il DNA fissato sulla matrice è subito pronto per la PCR. Se il DNA è richiesto in soluzione, usare GenSpin Plant la Whatman. Ideale per automatizzazione L utilizzo di sistemi automatizzati accelera le fasi di manipolazione di multiple FTA Cards, riducendo i tempi di lavoro e standardizzando la purificazione del DNA. I dischi prelevati dalla FTA Card possono essere sottoposti alle fasi di lavaggio e preparazione per la PCR su diverse tipologie di strumenti presenti in commercio (liquid handling robot). Tre semplici passaggi operativi per ottenere puro DNA da piante Purificare Depositare Campione Perforare un Disco di Campione Applicazioni Analisi del DNA di piante mediante saggi PCR Selezione di colture mediante marker genetici Identificazione delle varietà vegetali Analisi di filogenesi Amplificazione di loci genetici LCN (low copy number loci) Invader assay Multiple displacement amplification Identificazione di specie transgeniche

10 PROTOCOLLO APPLICATIVO DEL FTA PLANT DNA Dispensare il campione Depositare il campione vegetale o l omogenato sulla FTA Card. Lasciarlo asciugare completamente. Soluzione tampone TE-1 Lavare due volte con soluzione tampone TE-1 (10mM Tris, 0,1 mm EDTA, ph 8,0). Eliminare la soluzione tampone usata dopo ciascun lavaggio. INFORMAZIONI PER L ORDINE DAL CATALOGO WHATMAN No. Cat. Prelevare un piccolo disco (punch) dal Campione Prelevare mediànte perforazione un disco dalla matrice FTA impregnata di materiale vegetale. Purificazione con Reagente di Purificazione FTA (Purification Reagent FTA) Mettere il disco di campione in un tubo per PCR ed effettuare tre lavaggi con il Reagente di Purificazione FTA. Eliminare la soluzione utilizzata dopo ciascun lavaggio. Descrizione Essiccazione Lasciar asciugare il disco nel tubo PCR PCR Aggiungere la master mix per la PCR direttamente al disco e iniziare la reazione di amplificazione. Quantità per Confezione WB FTA Starter Pack 1 WB FTA Reagente di Purificazione 500mL WB FTA Classic Card (non-indicatrice) 100 WB FTA Mini Card (non-indicatrice) 100 WB FTA Micro Card (non-indicatrice) 100 WB FTA Gene Card 100 WB Harris Micro Perforatore 1.2mm 1 WB Harris Micro Perforatore 1.2mm Tip 1 WB Harris Uni-Core 1.25mm Perforatore 4 WB Busta Multi-Barrier (grande) 500 WB Busta Multi-Barrier (piccola) 500 WB FTA Gene Card/Busta/Essiccante 1000 WB FTA Classic Card/Busta/Essiccante 1000 WB mm Plunger di Ricambio 1 WB Essiccante (1g) 1000 WB Busta Postale FTA Card 50 WB Tagliere di Ricambio 1 WB Supporto per FTA Gene Card 20 WB GenSpin Plant Kit 50 Purificazioni SWB GenSpin Plant Kit di Prova 5 Purificatzioni Avete bisogno di DNA in soluzione? Questo kit di semplice utilizzo è ideale per la rapida preparazione di DNA a doppio filamento da piccole quantità di materiale vegetale, per analisi PCR. Il materiale raccolto dalle piante può essere omogeneizzato a temperatura ambiente e purificato in meno di 30 minuti. Qualità Whatman Whatman, azienda leader nelle tecnologie per la separazione, è nota nellà comunità scientifica per prodotti e soluzioni innovative contraddistinte da un elevata qualità. Il nostro istinto per la semplificazione accelera la velocità di fare nuove scoperte, riduce i costi e risparmia tempo. Inoltre, per accentuare ulteriormente l impegno nel soddisfare le specifiche esigenze dei nostri clienti, Whatman è organizzata in quattro aree distinte: LabScience, BioScience, MedTech Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono disponibili collegandovi al sito FTA, GenSpin e Whatman sono marchi registrati della Whatman Group. North America Europe Japan Asia Pacific Whatman Inc 9 Bridewell Place, Clifton, NJ Technical Support: Customer Service: Outside US: Fax: info@whatman.com Whatman International Ltd Springfield Mill James Whatman Way Maidstone Kent ME14 2LE, UK Tel: +44 (0) Fax: +44 (0) information@whatman.com Whatman Japan KK Daiwa Ueno Building 1F 6-10 Ueno 5-chome, Taito-ku Tokyo , Japan Tel: +81 (0) Fax: +81 (0) japaninfo@whatman.com Whatman Asia Pacific Pte Ltd 171 Chin Swee Road #08-01 San Centre Singapore Tel: Fax: wap@whatman.com GenSpin Plant Kit di Purificazione DNA Whatman Leaders in Separations Technology Cat No. S

11 FTA Author Title Year Publication Comment/Summary Keywords Aranda et al. FTA Technology, Unique Formats for 2001 Poster: Promega 12th Demonstrates FTA for downstream processes such as DNA elution, hair root, the Collection, Shipment, Archiving and Processing of Biological Samples International Symposium on Human ID from FTA, hair digestion, STR, extract DNA, microsatellites, human id, forensics multiplex PCR for human identification. Sample types buccal, human, blood, include hair roots, buccal cells, and blood restriction digest DNA Aranda et al. A Simple Device for the Efficient Transfer of Buccal Swab Cells onto FTA Paper 2003 Poster: Promega 14th International Symposium on Human ID, Phoenix AZ Describe how the SAMPACT device works. SAMPACT is a plastic cassett containing an FTA card that induces and equal pressure on a foam swab to get even transfer of buccal cells onto the FTA card forensic, databasing, human id Aranda et al. Alkaline Extraction of DNA from FTA Paper Spotted with Buccal Epithelial Cells and Whole Blood 2004 Poster: Promega 15th International Symposium on Human ID, Phoenix AZ Alkaline conditions were used to extract DNA from FTA DNA in solution, real time cards with blood or buccal cells applied to them. Extracted PCR, extracted DNA DNA was quantitated using the real time PCR kit Quantifiler from ABI. For blood samples extracted DNA from a 3mm punch ranged from 74 to 206 ng/punch; 7mm punches ranged from 165ng to 1.57ug. For buccal samples extracted DNA from a 3mm punch ranged from 35-52ng/punch; 7mm punches ranged from ng/punch. Babu Standing Orders - Institute of Aviation Medicine Aircrew DNA Repository 2002 Royal Australian Air Force Frame work as to how to administer and maintain aircrew's DNA repository for use in identification following an aircraft accident. How and when to retrieve archived samples. DNA repository, military, air force, disaster victim identification, blood samples, storage conditions, human id Baron et al. Parentage Testing and Effect of Misidentification on the Estimation of Breeding Value in Gir Cattle Genetics and Molecular Biol 25(4): FTA used to collect blood samples from cattle. 2mm PCR, microsatellites, punches were washed and the DNA amplified by PCR in cattle, blood, parentage, 40 ml reactions directly. Also DNA from 6mm FTA punches genetic identity, breeding, were extracted using Chelex. dairy cows, Gir, genetic value of bulls, animal biotechnology Barton, JC, R Swada-Hirai, BE Rothenberg and RT Acton Two Novel Missense Mutations of the HFE Gene (I105T and G93R) and Identification of the S65C Mutation on Alabama Hemochromatosis Probands Blood Cells, Molecules and Diseases 2 5(9) Note: a proband is a person with hemochromatosis a disease where there is too much iron in the blood, giving blood is a "treatment". Genomic DNA from saliva samples collected from volunteers on FTA for this study. Regions of the HFE gene were amplified by PCR then analyzed by sequencing and dot-blot hybridizations. human, population study, blood disease, saliva, buccal cell, FTA Reference DatabaseJun05 1

12 Beck et al. Beck and Frenkel Simple, Sensitive, and Specific Detection of Human Immunodeficiency Virus Type 1 Subtype B DNA in Dried Blood Samples for the Diagnosis in Infants in the Field Genotyping Kits for the Detection of HIV-1 pol Drug Resistance Mutations by an Oligonucleotide Ligation Assay 2001 Journal Clinical Microbiology 39: NIH AIDS Research and Reference Reagent Program Easy collection in the field for HIV testing in a central lab. Depicts High Sensitivity. Instructions for a kit to analyze HIV pol DNA. Whole blood collected on FTA (pg 14) can be used in the oligonucleotide ligation assay (OLA). These instructions also provide a good overview as to what the OLA is; briefly OLA is a way to identify a point mutation in a gene. A segment of the DNA is amplified by PCR then 2 oligonucleotides are hybridized to the PCR product. The 2 oligos are then ligated with a ligase enzyme. Only when a specific mutation is present will the oligos ligate and are detected. archiving, human, blood, PCR, HIV, sensitivity, specific, diagnosis, infection, pol, dried blood spots, diagnostics point mutation, PCR, HIV, pol gene, diagnostics Becker et al. Real-time PCR for detecting Trypanosoma brucei in human blood samples 2004 Diag. Microbiol. Inf. Dis. 50: Blood spiked with dilutions of cultured T. brucei and blood from patients with sleeping sickness were spotted to FTA cards. Punches of 2.0mm were washed according to the standard protocol then the DNA extracted using Chelex 100 resin. For real time PCR 4µl of the Chelex supernatant were included in the 10µl PCR mix. pathogens, disease diagnostics, human, bacteria, real-time PCR Belgrader & Automated Sample Processing Using Marino Robotics for Genetic Typing of STR Polymorphisms by Capillary Electrophoresis Belgrader et al. Automated DNA Purification and Amplification from Bloodstain Cards Using a Robotic Workstation Bever et al. Implementation of Laboratory Automation for the analysis of STR loci 1997 Lab. Robotics & Automation 9: 3-7 Use of FTA to rapidly produce template suitable for genotyping ID on an automated platform BioTechniques 19: Blood sample processing on FTA using an automated (Beckman) platform Poster 8th International Symposium on Human ID, The isolation of DNA using FTA & Rosys robotic workstation. robotic, automation, PCR, high-throughput, HT, blood, human, 96- well, human id, forensics, automation robotic, Biomek 1000, blood, human, automation human, blood, PowerPlex, DNA Plate, human id, forensics, automation Beyrer et al. Molecular Epidemiology of HIV-1 Among Northern Thai Drug Users: Implications for Vaccine Efficacy Trials Poster 10th Conference on Retroviruses and Opportunistic Infections FTA used to collect blood samples from drug using volunteers in an HIV study. HIV, genetic diversity, infection, virus, diagnostics, population study FTA Reference DatabaseJun05 2

13 Bhattacharya et Use of Reverse Transcription and al. PCR to Discriminate Between Infectious and Non-Infectious Hepatitis A Virus J Virological Meth. 116: Hepatitis A virus (an RNA virus), released into cell culture RT-PCR, RNA, virus, media from infected cells was applied to FTA cards. The environmental, food RNA was eluted from the cards in Tris-EDTA buffer pathogen, food safety, containing 2-mercaptoethanol then preciptated with isopropanol in the presence of carrier trna. RNA was also prepared using Trizol. Detection was more sensitive for RNA prepared on FTA than with Trizol. Bilyeu KD and Beuselinck PR Biondi et al. Birus et al. Genetic Divergence between North American Ancestral Soybean Lines and Introductions with Resistance to Soybean Cyst Nematode Revealed by Chloroplast Haplotype Forensic Sample Processing using a Robotic Workstation: Automated Paper-Based Spotting of Whole Blood convicted Offender Samples and High Throughput DNA Isolation for STR Analysis. How High Should Paternity Index Be for Reliable Identification of War Victims by DNA Typing? 2005 Journal of Heredity doi: /hered/esi Poster Promega 14th International Symposium on Human Identification Study was to examine genetic diversity in soybean genotypes present in USDA collection using SSRs in the chloroplast genome. Leaf tissue was pressed onto FTA cards or DNA from leaf tissue was prepared using Qiagen Plant mini kit or Promega Wizard Magnetic 96 Plant kit. 1 FTA punch or ng of DNA was included in each PCR amplification. MultiPROBE II Forensic workstation used to spot whole blood to FTA Gene Card in Gene Card tray for long term archiving. Shows how FTA and automation fit into forensic lab work flow Croat Med J 44: FTA used to collect blood samples from relatives of missing persons for genomic DNA extraction. Soybean, germplasm collection, chloroplast DNA, Gene Card, blood, automaton, forensic, human ID human, missing persons, ID, STR, PCR, skeleton, genetic match, chelex, genotyping, databasing, human id, forensics Blair et al. Evidence of Rickettsial and Leptospira Infections in Andean Northern Peru 2004 Am. J. Trop. Med. Hyg. 70(4): Blood collected from study participants in vacutainer tubes with some being spotted onto FTA for genomic DNA analysis of bacteria from the genus Rickettsia. A nested PCR method was used to identify a Rickettsia specific gene, htra. field collection, human, blood, bacterial infections, environmental, diagnostic, population study Borys et al. Borys. S PCR Volume Reduction Study Using Bloodstained FTA Collection Cards and Capillary Electrophoresis Evaluation of High Throughput STR Technologies for Potential Implementation in the National DNA Databank 1998 Poster Promega 9th Annual Int Symp Of Human ID MSc. Thesis Ottowa-Carleton Institute of Biology, Carleton University, Ottowa Canada Final PCR volume of 5µl gives good, reproducible, STR profiling results; cycles reduced form 25 to 23. Use of FTA to collect blood, buccal cells for DNA analysis and databasing. Reducing PCR from 25 ml to 5 ml increases sensitivity 9-fold. blood, buccal, human, RCMP, STR, PCR, forensics, human id DNA bank, repositories, database, DNA typing, genotyping, human, blood, buccal, databank, forensics, human id FTA Reference DatabaseJun05 3

14 Bosman et al. Reverse Line Blot: A diagnostic tool to detect blood parasites Presentation: 31 st Annual Conference of the Parasitological Society of Southern Africa (abstract # Blood from various animal species was applied to FTA or collected in vacutainers with various anti-coagulants. DNA was extracted or FTA punches prepared with the standard procedure and analyzed by PCR for the presence of Babesia or Theileria parasites. Parasites, whole blood, animal, PCR, animal biotechnology 17) Both et al. FTA Paper, DNA, Time, and the Profiler 2000 Web Article from Forensic Science Center Adelaide, Australia Review of FTA for the collection & archiving of offender blood samples.. RT storage for 9 years w/out signal loss. Punches from blood stained FTA could be PCR'd without washing, but punches containing buccal DNA typing, blood, buccal, human, archive, PCR, STR, integrity, human id, forensics cells required washing for successful PCR. ABI ProfilerPlus PCR kit was used in this study. Budowle et al. DNA Typing Protocols: Molecular Biology and Forensic Analysis BioTechniques Books Publication, Eaton Publishing, Natick MA Description of FTA as a sample collection medium for blood and buccal cells. Protocol for preparing FTA punch for RFLP or PCR-based analysis. Shows photos of CEP swab aka OmniSwab and Gene Guard Swab aka genomic DNA, forensic, DNA typing, PCR, CEP swab, human id, forensics Foam tipped applicator for collecting buccal cells. Burgoyne Convenient DNA Collection and Processing: Disposable Toothbrushes and FTA Paper as a Nonthreatening Buccal-Cell Collection Kit 1997 Poster Promega 8th International Symposium on Human ID Use of a cytobrush to collect buccal cells to deposit on FTA buccal cell, human, buccalbrush, food coloring, forensics, human id Compatible with Automatable DNA Burgoyne & Hallsworth Processing Studies with FTA 1995 Presentation 5th International Symposium on Human ID FTA kills HSV within 5 sec. FTA prevents fungal growth on blood spots held at body temp and high humidity. FTA can be used to detect HSV, CMV (DNA viruses) and Hepatitis C (RNA virus) and protect samples from accelerated and natural aging. HSV, Herpes Simplex Virus, 903, 3MM, FTA923, Cytomegalovirus, CMV, Hepatitis C, DNA virus, RNA virus, diagnostics Castilho et al. DNA Template Preparation and Archiving for Blood Group Genotyping of Remotely Located Patient Populations 2001 Poster: American Association of Blood Banks, San Antonio 2001 FTA cards were used for blood group genotyping using whole blood, plasma, and amniotic fluid. The cards were used for collection in remote areas and shipped back to central lab for testing. storage, transportation, human, PCR, RFLP, population study, human id, diagnostics FTA Reference DatabaseJun05 4

15 Castilho et al. Genotyping for DO A/DO B Facilitates Transfusion of Sickle Cell Disease Patients 2001 Poster: American Association of Blood Banks, San Antonio 2001 Blood samples from patients who received a transfusion was spotted to FTA, shipped back to central lab for PCR analysis. Dombrock gene, RFLP, human, blood, genotyping, population study, human id, diagnostics Cerda-Flores et al. Maximum Likelihood Estimates of Admixture in Northeastern Mexico Using 13 Short Tandem Repeat Loci Am J Hum Biol 14: Blood from people in Northeastern Mexico were collected and FTA and analyzed by PCR for 13 STRs. Data was analyzed to determine lineage contributions from Europe, American Indian and African origin. PCR, 1 mm punch, STR, human, blood, population study, human id Chappuis et al. Chu et al. Options for Field Diagnosis of Human African Trypanosomiasis Survey of Nepalese Animals for the Presence of Cyclospora Cayetanensis 2005 Clin. Microbiol. Rev. 18(1) Poster: Am Assoc. Microbiology, Washington DC FTA is described for collecting blood, lymph node fluid or CSF for detection of trypanosome DNA. FTA is preferred over untreated filter paper because FTA protects DNA from degradation. FTA used to collect animal fecal samples to detect protozoan parasites protozoa, protozoan, microorganism, disease diagnostics, PCR, disease diagnostics, fecal samples, animals, detection, PCR, nested PCR Chu et al. Detection of Cyclospora Cayetanensis in animal fecal isolates from Nepal using and FTA filterbase polymerase chain reaction method 2004 Am. J. Trop. Med. Hyg 71(4): FTA was used to collect fecal samples of dogs,chickens and monkeys to detect protozoan parasites capable of being transmitted to humans and causing diarrheal illnesses. Fecal samples were collected in 2.5% potassium dichromate (to stabilize the protozoan oocytes). Stool specimens 10-20ul were spotted disease diagnostics, fecal samples, animals, detection, PCR, nested PCR directly onto FTA and dried on a hot block at 56 o C. The disks were washed as usual then amplified. Connolly et al. Automated methods for processing samples stored on FTA paper 1997 Poster 11th International Symposium on Human ID, Biloxi, LA FTA has proven itself to be a robust & versatile method for collection, storage & analysis of DNA. With (semi-) automated methods is capable of high- throughput analysis. plate washers, throughput, automation Connolly et al. D12 archiving & recovery of bacterial plasmids using FTA paper 1999 Poster 13th International Mouse genome Conference Archiving & recovery of clones ranging from 2Kb to 227Kb. plasmid DNA, freezer, storage, CloneSaver, 96- well, M13 sequencing, genomics FTA Reference DatabaseJun05 5

16 Crabbe A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals J. Biochem. Biophys. Methods 57: Both the polyps (the soft living part of coral, about the size of a pencil eraser) and the symbiotic algae (zooxanthellae) that are part of coral colonies were collected and put on FTA. Fragments were ground and applied to FTA. DNA was extracted using Qiagen DNeasy. Ribosomal genes were PCR amplified for species identification. FTA excellent for field collection and transport of samples from tropical locations. coral, algae, identification, ribosomal genes, extracted DNA, tropical, field collection, room temperature transport, plant Davis et al. DNA Tests in Prolific Sheep from Eight Countries Provide New Evidence on Origin of the Booroola (FecB) Mutation Biology of Reproduction 66: Blood samples from sheep collected onto FTA for DNA preparation. FTA disks (1.2mm) were amplified by PCR and the amplicons subjected to RFLP analysis. PCR amplicons prepared from FTA disks containing sheep DNA were also subjected to TaqMan Assays. animal biotechnology, blood, mutation analysis Davis, et al. A Novel Genvault Multiwell Plate Format for Whatman FTA in Robotic, High Throughput DNA Archiving 2003 Poster: Society for BioMolecular Screening Meeting Describes the Genvault system and the EasyClone 384 well plate EasyClone 384 well Plate Del Rio Cost Effectiveness in Sample Processing using the FTA Treated Stain Card for High Throughput 2001 Poster from Promega 8 th International Symposium on Human Identification Important for showing the cost-effectiveness of FTA for blood collection and processing compared to vacutainer collection. offender specimens, archive, collection, DNA database, transport, sample tracking, human, blood, buccal, human id, forensics Del Rio et al. Del Rio, S. Reusing the Same Blood-Stained Punch for Sequential DNA Amplifications and Typing Amplification of DNA from Bone Marrow Aspirate and Cervical Smears on FTA Blood Stain Cards 1996 BioTechniques 20: Demonstrates that not all NA bound to FTA cannot be removed with heat, and punches can be re-used for many different PCR amplifications multiple times (4 sequential runs) 1997 Application Note from Fitzco Different sample types than blood processed successfully on FTA (either spotted or brushed on the cards) human, genotyping, databanks, human id, diagnostics, forensics, research diagnostic tests, diagnostics, Del Rio- LaFreniere et al New Method for Collection and Detection of K-ras Point Mutations from Tissue Specimens for Clinical Diagnostic Applications 1998 Poster: AMP Meeting Washington DC Samples of pancreatic tissue, ductal brushings, cells, cancer research, tissue fine needle aspirates or fluid were applied to FTA. FTA screening for mutation, cards were pressed to tissue samples. A 3mm punch diagnostics was taken and washed as normal. PCR master mix was added to the washed punch to detect mutation in the K- ras gene. Bile and fine needle aspirates showed the weakest signal on FTA but the others gave good PCR bands on gels. FTA Reference DatabaseJun05 6

17 Del Rio- LaFreniere SA, McGlennen RC A Unique Method of Detection of the Prothrombin 20210A (FactorII) Mutation Using the Simultaneous Allele Specific Amplification (SASA) 1998 Poster: 13th San Diego Conference on DNA Technologies in Human Detection Human Blood sample were collected on FTA and washed 3mm punches subjected to a specific PCR amplification procedure for detecting a mutation in the prothrombin gene. disease diagnostics, PCR, mutation research, Devost & Choy Mutation Analysis of Gaucher Disease Using Dot-Blood Samples on FTA Filter Paper 2000 American Journal of Medical Genetics 94: Polymorphism detection of alleles implicated in Gaucher Disease on population bloods collected & stored on FTA. diagnostic tests, RFLP, mutation, genotyping, nested PCR, human, diagnostics, population study Dobbs et al. Dutton and Tieber Drescher & Graner Use of FTA Gene Guard Filter Paper for the Storage and Transportation of Tumor Cells for Molecular Testing A Modified Protocol for Sex Identification of In Ovo Avian Embryos and its Application as a Management Tool for Endangered Species Conservation Programs. PCR-genotyping of barley seedlings using DNA samples from tissue prints 2002 Archives Pathology and Laboratory Medicine 126: Shows FTA for tumor cell collection, room temperature storage, transport, and DNA testing. Compares FTA with a Qiagen method J Zoo Wildlife Med 32(2)176-Small amounts of blood were collected by syringe from blood vessels in developing bird eggs. The blood/fluid was 180. spotted to FTA and analyzed to determine the gender of the embryo Plant Breeding 121, Method to sample barley leaves for PCR analysis using FTA. Compares FTA to CTAB standard method; achieve comparable results quicker and easier. pathology, cancer, lymph node, cell suspensions, lung, breast, endometrium, ovary, kidney, thyroid, research, DNA banking blood, animal biotechnology, birds, chicken, pigeon, owl marker assisted breeding, plant, screening, leaves, high throughput, genotyping, field collection Dyer et al. Detection of Bacilli Spores Using PCR and FTA Filters 2003 Poster 103rd General Meeting American Society for Microbiology FTA used to isolate DNA from Bacilli spores for nested PCR detection. spores, Bacillus, food, clinical, environmental, Elliot Isolation of DNA Using FTA Blood Stain Collection Cards 1998 Poster 7th Annual International Symposium of Human ID FTA for forensic collection, archive, rapid purification tool. 9 months RT archiving. Did not let card dry after blood application, put it wet into pouch with desiccant without effecting PCR. RCMP, rapid collection, rapid extraction, human, saliva, buccal, STR, blood, storage conditions, forensics, human id FTA Reference DatabaseJun05 7

18 Elliot et al. Extraction of DNA from FTA Blood Stain Collection Cards for Construction of a Large STR National DNA Data Base 1997 Poster 8th International Symposium on Human ID, FTA tested for DNA stability, yield (peak height on a gel) ease of use and consistency with blood fresh, frozen, saliva and semen. Did not have good luck with purified DNA on FTA with standard wash protocol. RCMP, rapid collection, rapid extraction, human, saliva, buccal, STR, blood, storage conditions, forensics, human id Expert et al. Evaluation of Two Techniques for Extraction and Conservation of DNA of Various Quarantine Bacteria 2004 Poster EPPO Conference on Quality of Diagnosis and New Diagnostic Methods for Plant Pests. Noordwijkerhout, NL Extraction of DNA from plant disease bacteria by FTA compared to Q Biogene kit. Both methods able to detect gram (-) bacteria at low levels seen in plants. Both methods could only detect gram (+) bacteria at higher concentrations. plant disease, microbes, Fici et al. Sequence Based Typing of Blood Spots on Filter Paper After Extended Storage Forrest et al. Polymorphism at the Ovine b3- Adrenergic Receptor Locus: Associations with Birth Weight, Growth Rate, Carcass Composition and Cold Survival. Forrest et al. Two Rare Polymorphisms in the D8S1179 and D13S317Markers and Method to Mitigate Their Impact on Human Identification 1998 Poster Human Immunology Blood and lymphocyte samples spotted to FTA cards 59(1) 141 then processed for HLA & blood typing after long-term storage. PCR from FTA paper more robust than PCR from untreated filter paper. A treatment with Proteinase K (5 ml 10 mg/ml prok added to 495 ml FTA purification reagent containing punch; incubate 1 60 o C)was required to amplify one class of the HLA Animal Genetics 34: FTA used to collect blood from lambs. DNA on FTA punches were examined via PCR for differences in the sequence in the b3-adrenergic receptor gene which were then compared to phenotypic traits of the sheep Croat Med J 45: Blood collected onto FTA and used in human genotyping with the commercially available kits. The researchers saw a new peak in two of the loci, sequenced the DNA and found DNA base changes that affected the STR profile. They make suggestions as to what that means for identifying humans in forensic cases. untreated filters, spin basket, 30 months, 2.5 years storage, Proteinase K, diagnostics, population study PCR-SSCP, polymorphisms, body weight, marker assisted breeding, animal ID, animal biotechnology human id, forensics, DNA polymorphisms, DNA sequencing, STR Fox et al. RT-PCR from Eukaryotic Cells Stored on FTA Archival Paper 2000 Poster Abstract: Plant and Animal Genome VIII San Diego Demonstrates FTA for collection, storage, processing of mrna for RT-PCR- mrna from human cells spotted onto FTA was stable for 1-2 mos room temp or > 10 mos at - 20 o C or -70 o C. RNA extraction, plant, BHK21 cells, HeLa cells, high copy number mrna, low copy number, FTA Reference DatabaseJun05 8

19 Franchina et al. Fujiwara et al. Fyfe et al. Polymorphism of the CD30 Promoter Microsatellite Repressive Element is Associated with Development of Primary Cutaneous Lymphoproliferative Disorders. Plasma DNA Microsattelites as Tumor-specific Markers and Indicators of Tumor Progression in Melanoma Patients. Assessment of trypanosome prevalence in FITCA high risk areas Cancer Epidemiol Biomarkers Prev 14(5) Blood from patients was collected on FTA for PCR amplification of sections of the CD30 gene Cancer Res. 59: Blood collected from 76 cancer patients and 20 healthy donor volunteers and spotted to FTA for genomic DNA extractions and long term storage ICPTV Newsletter 8 pg September 2003 FITCA is an EU funded project to control tsetse infestation in Uganda. Blood from cattle ( ) ear veins was collected directly onto FTA cards and transported back to the lab for analysis by PCR for 3 species of Trypanosome. Detection of parasite by PCR is significantly more sensitive than detection by microscopy. population study, cancer research cancer research, tumor progression, Loss of heterozygosity, human, diagnostic cattle, DNA, PCR, parasite, animal biotechnology, environmental Gaytmenn et al. Determination of the sensitivity and specificity of sibship calculations using AmpFI STR Profiler Plus Int J Legal Med 116: Buccal cell samples were collected on FTA and used as template for the 9 STR Profiler Plus loci. These data were used to determine the likelyhood ratios of paternity and sibling relationships. Buccal cells, human, STR, paternity, sibling, population study, human id, forensics Goldsborough et al. Room Temperature Archiving of Plasmid Clones in an Automatable 96- Well Format 2000 Poster Abstract: Plant and Animal Genome VIII San Diego, USA Demonstrates use for BAC and plasmid clones. glycerol stocks, overnight cultures, resuspended colonies, purified DNA, 2 Kb, 200 Kb, CloneSaver, genomics Gutierrez- Corchero et al. Using FTA cards to store avian blood samples for genetic studies. Their application in sex determination 2002 Molecular Ecology Notes 2: FTA cards were used to collect, store, and analyze blood from nestling and adult birds (Great Grey Shrikes) for sex determination. birds, DNA banks, wildlife ecology, PCR, population studies, blood, nucleated red blood cells, animal biotechnology Halbert et al Conservation Genetic Analysis of the Texas State Bison Herd 2004 J Mammalology 85(5): Blood samples were collected on FTA. Single 1.2mm punches were used in multiplex PCR amplifications in 5ul. animal ID, population study Hanes et al. Inactivation of Cryptosporidium parvum Oocytes in Fresh Apple Cider by UV Irradiation 2002 Appl Envir Microbiol. 68(8) Apple cider containing parasitic oocytes was spotted on FTA and analyzed by PCR. gastroenteritis, fecal pellets, tissue, parasites, infection, environmental, food safety FTA Reference DatabaseJun05 9

20 Hansen and Blakesley Simple Archiving of Bacterial and Plasmid DNAs for Future Use 1998 Focus 20(3) Bacterial genomic DNA was stored on an FTA card with successful PCR amplifications. Also, plasmid DNA was stored and transformed on FTA. Agrobacter tumefaciens, plant bacteria, E. cole, gram negative, gram positive Streptomyces, colonies, clones, genomics Hartman and Lampel Long Term Room Temperature Storage and Detection of Norovirus on FTA Filter Paper Poster: 104th General Meeting of the American Society of Microbiology, New Orleans LA. Norwalk-like virus is a food borne pathogen. Norovirus is a Virus, RT-PCR, food leading cause of non-bacterial gasteroenteritis. Diagnosis borne pathogen, is almost exlusively done by detection of virus in stool detection, stool, feces, samples. Norovirus positive stool was diluted 1:10 with vomit, rapid analysis PBS and 200ul applied to FTA cards and dried overnight. Cards were stored in plastic Whirl-Pak bags for 6 and 12 months at room temperature. One sample was stored for 2 years. A 6mm disk of FTA (approx 6-7ul stool) was washed with 140ul sterile dist H 2 O. Viral RNA extracted with QiaAmp Viral RNA Mini Kit (Qiagen). All samples analyzed from FTA showed positive signal for Norovirus. FTA makes sample collection, storage and transportation easy and can be used to quickly detect Norovirus outbreaks. FTA can facilitate epidemiological investigations. Harvey, ML An alternative for the Extraction and Storage of DNA from Insects in Forensic Entomology. Henderson et al. The long term outcome of limbal allografts: the search for surviving cells J Forensic Sci. 50(3) Br J Ophthalmol 85: Samples of various life stages of flies were applied to FTA. A 3 fragments of 320, 650 and 1270 bp of a gene encoded in the mitochondrial DNA were amplified by PCR to show the quality of the DNA. Authors found the 1.2mm punch to be optimal for PCR amplification; 2.0mm punch provided FTA disks were pressed against the corneas of eyes receiving limbal allografts to collect cell samples. The DNA from the cells were typed using 4 human STR markers to determine if they derived from the donor or the recepient. insects, forensics, entomology, mtdna impression cytology, human identification, ID, clinical, tissue grafts, diagnostic Hernandez et Comparative Molecular Study of al. Populations of Common Crossbill (Loxia curvirostra ) on the Iberian Peninsula and the Baleric Islands. Hernandez et al. Identification of Lanius Species and Subspecies using Tandem Repeats in the Mitochondrial DNA Control Region Workshop WS02-P3 4th Conference of the European Ornithologists' Union Blood samples from birds collected onto FTA cards and prepared for PCR amplification of microsatellite markers Ibis 146: Mitochondiral DNA analyzed from bird blood spotted to FTA microsatellites, avian, blood, animal biotechnology birds, mitochondiral DNA FTA Reference DatabaseJun05 10