From Scientific Workflow Patterns to 5-star Linked Open Data
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1 From Scientific Workflow Patterns to 5-star Linked Open Data Alban Gaignard Hala Skaf-Molli Audrey Bihouée Nantes Academic Hospital (CHU de Nantes), CNRS, France LINA, Nantes University, CNRS, France Institut du Thorax, Nantes University, INSERM, CNRS, France 8th USENIX workshop on Theory and Practice of Provenance (TaPP 16) Washington DC
2 Needs for linked experiment reports 2
3 Motivations: reusing (massive) RNA-seq data TopHat: algorithm to align multiple sequence reads to a reference genome (known genes). 3
4 Motivations: reusing (massive) RNA-seq data TopHat: algorithm to align multiple sequence reads to a reference genome (known genes). 1 sample Input data 2 x 17 Gb 1-core CPU 170 hours 32-cores CPU Output data 32 hours 12 Gb 4
5 Motivations: reusing (massive) RNA-seq data TopHat: algorithm to align multiple sequence reads to a reference genome (known genes). 1 sample 300 samples Input data 2 x 17 Gb 10.2 Tb 1-core CPU 170 hours 5.9 years 32 hours 14 months 12 Gb 3.6 Tb 32-cores CPU Output data 5
6 Motivations: reusing (massive) RNA-seq data TopHat: algorithm to align multiple sequence reads to a reference genome (known genes). 1 sample 300 samples Input data 2 x 17 Gb 10.2 Tb 1-core CPU 170 hours 5.9 years 32 hours 14 months 12 Gb 3.6 Tb 32-cores CPU Output data Challenges Algorithmic performance, storage, preservation, reuse (limit recompute) & share. 6
7 Motivations: reusing experiment results Scientific experiment: RNA sequencing to quantify gene expression levels under multiple biological conditions. 7
8 Motivations: reusing experiment results Scientific experiment: RNA sequencing to quantify gene expression levels under multiple biological conditions. Need for scientific context : metadata 8
9 Expected result: human+machine tractable reports Annotated Material & Methods Links to some workflow artifacts (algorithms, data) 9
10 5-star Linked Open Data W3C standards for machine and human readable data on the web. : time and expertise! 10
11 5-star Linked Open Data W3C standards for machine and human readable data on the web. : time and expertise! How to ease this process? Workflow engines automation PROV workflow runs as linked data 11
12 PROV only 12
13 PROV only too fine-grained no domain concepts 13
14 Provenance as a Linked Experiment Report few + meaningful statements 14
15 Problem statement & objectives Problem statement Scientific workflows produce massive raw results. Their publication into curated query-able linked data repositories requires lot of time and expertise. Can we exploit provenance traces to ease the publication of scientific results as Linked Data? 15
16 Problem statement & objectives Problem statement Scientific workflows produce massive raw results. Their publication into curated query-able linked data repositories requires lot of time and expertise. Can we exploit provenance traces to ease the publication of scientific results as Linked Data? Objectives (1) Leverage annotated workflow patterns to generate provenance mining rules. (2) Refine provenance traces into linked experiment reports. 16
17 Rules generation 17
18 Approach 18
19 Input domain-specific annotations (❶,❷) Workflow patterns ❶ Sequence patterns, with possibly intermediate steps P-PLAN ontology: Step, Variable, hasinputvar, hasoutputvar EDAM ontology: hasfunction, RNA sequence, Genome map 19
20 Input domain-specific annotations (❶,❷) Workflow patterns ❶ Sequence patterns, with possibly intermediate steps P-PLAN ontology: Step, Variable, hasinputvar, hasoutputvar EDAM ontology: hasfunction, RNA sequence, Genome map Experiment report template ❷ Link scientific claims, statements, material and methods MicroPublication ontology: Material, Method, Claims Experimental factor ontology: Transcriptome, Gene expression NCBI taxonomy: Homo Sapiens Open Annotation model: hasbody, hastarget 20
21 PoeM: generating PrOvEnance Mining rules ❸ (SPARQL Property path) (SPARQL Basic graph pattern) (SPARQL Construct query) 21
22 PoeM: sample generated rule ❸ > n e Th rt a p < > f I < rt a p 22
23 First experiments & results 23
24 Experiment context SyMeTRIC: systems medicine project ( , call Connect Talent ), funded by the french region Pays de la Loire. 24
25 Experiment Material & methods Real-life RNA-seq workflow to study 3 mice populations WF implemented in Galaxy, run on 2 biological samples PROV traces exported from Galaxy Histories (API) 25
26 Experiment Material & methods Real-life RNA-seq workflow to study 3 mice populations WF implemented in Galaxy, run on 2 biological samples PROV traces exported from Galaxy Histories (API) Results (for 1 biological sample) 60h CPU (12 cores for genome alignment), 21Gb storage 3s to export 81 PROV triples from the Galaxy history 2s to apply the rule and produce 35 Micropublication triples 26
27 Conclusion & perspectives Semi-automated approach (1) PoeM generates semantic web rules (2) PoeM rules applied on PROV traces to assemble linked experiment reports (MicroPublication) Limitations: - Sequence workflow patterns only - SPARQL property paths with complex WF patterns? - Syntactic matching between WF patterns and PROV labels Usage scenarios: Query workflow datasets with domain concepts Populate RDF repositories with WF results 27
28 Conclusion & perspectives Future works (1) WF patterns: split-merge, common motifs (2) Genericity: other domains / other reports (RO, Nanopub.) (3) PROV heterogeneity: multi-systems PROV (4) Evaluation: involving biologists, at larger scale 28
29 Questions? Demo: Contact: Acknowledgments BiRD bioinformatics facility Connect Talent Call
30 Larger scale experiments (PROV traces) 1232 edges A. Gaignard, H. Skaf-Molli, A. Bihouée TaPP 16 30
31 Larger scale experiments (PROV traces) 49 edges A. Gaignard, H. Skaf-Molli, A. Bihouée TaPP 16 31
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