2 Molecular Biology Kits

Transcription

1 56 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

2 BBI Catalogue Plasmid DNA, Gel Extraction & PCR Products Purification Kits Code Description Size B60363(BS363) EZ-0 Spin Column PCR Products Purification Kit $ B60363(BS364) EZ-0 Spin Column PCR Products Purification Kit 00 Preps $ B630663(BS664) B80365(BS365) EZ-0 Spin Column PCR Products Purification Kit 96-Well Plate PCR Products Purification Kit XPlates $ $ 3.5 B80365(BS365) B60367(BS367) B60367(BS368) B60367(BS668) B80369(BS369) B80369(BS369) B60353(BS353) B60353(BS354) B630653(BS654) B6043(BS439) 96-Well Plate PCR Products Purification Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Cleanup Mini-preps Kit 96-Well Plate DNA Cleanup Mini-preps Kit 96-Well Plate DNA Cleanup Mini-preps Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column DNA Gel Extraction Kit EZ-0 Spin Column Plasmid DNA Mini-preps Kit 5 X Plates 00 Preps XPlates 5 X Plates 00 Preps $ 6.50 $ $ $ $ 3.5 $ 6.50 $ $ $ $ B6043(BS449) EZ-0 Spin Column Plasmid DNA Mini-preps Kit 00 Preps $ B6043(BS649) EZ-0 Spin Column Plasmid DNA Min-preps Kit $ B604(BS804) Ultra-Fast Spin Column Plasmid DNA Min-Preps Kit $ 5.50 B60465(BS4654) EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 4 Preps $ B60465(BS466) EZ-500 Spin Column Plasmid DNA Maxi-preps Kit 0 Preps $ B8045(BS45) 96-Well Plate Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B8045(BS45) 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B8008(BS908) Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B8008(BS909) Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B80043(PT9043) 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit X Plates $ 3.5 B80043(PT9044) 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit 5X Plates $ 6.50 B698(BS798) EZ-0 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit $ 0.90 B549(SK49) EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 4 Maxi-Preps $ 90.7 B549(SK50) EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit 0 Maxi-Preps $ B60467(BS467) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit 0 Preps $.00 B60467(BS468) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit $ B60467(BS469) EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit $ Genomic DNA Isolation Kits Code Description Size A8080(BS80) EZ-DNA Reagents 00 Preps $ 5.50 B80843(BS843) One-Tube Bacteria Genomic DNA Isolation Kit 00 Preps $ B80843(BS844) One-Tube Bacteria Genomic DNA Isolation Kit 500 Preps $ B585(BS85) Rapid Bacteria Genomic DNA Isolation Kit $ Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 57

3 B6043(BS43) EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit $ 44.0 B80064(BS64) EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit $ B809(SK9) 96-Well Plate Bacteria Genomic DNA Mini-Preps Kit X Plates $ B8095(SK95) 96-Well Plate Bacteria Genomic DNA Mini-Preps Kit 5X Plates $ B587(BS87) Rapid Yeast Genomic DNA Extraction Kit $ B80857(BS857) EZ-0 Spin Column Yeast Genomic DNA Mini-Preps Kit $ B808357(BS8357) B8045(FT745) B800(FT80) B80037(PT9037) B8086(PT786) B8086(PT787) B80045(BS45) B80045(BS66) B80035(PT9035) B80035(PT9036) 96 Well Plate Yeast Genomic DNA Mini-Preps Kit Rapid Fungal Genomic DNA Isolation Kit EZ-0 Spin Column Fungal Genomic DNA Mini-Preps Kit EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit One-tube Plan Genomic DNA Extraction Kit One-tube Plan Genomic DNA Extraction Kit EZ-0 Spin Column Plant Genomic DNA Mini-Preps Kit EZ-0 Spin Column Plant Genomic DNA Mini-Preps Kit EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit X Plates 4 Preps 00 Preps 4 Preps 0 Preps $ $ $ 44.0 $ 86.0 $ 5.50 $ 0.00 $ 44.0 $ $ 86.0 $ B80836(BS836) 96-Well Plate Plant Genomic DNA Mini-Preps Kit X Plates $ B8036(ST836) EZ-0 Spin Column Soil DNA Mini-Preps Kit $ 44.0 B5840(BS840) One-Tube Tissue DNA Extraction Kit 00 Preps $ B5840(BS840) One-Tube Tissue DNA Extraction Kit 500 Preps $ B804780(AT4780) Rapid Animal Genomic DNA Extraction Kit 0 Preps $ 6.5 B804780(AT478) Rapid Animal Genomic DNA Extraction Kit $ B804780(AT478) Rapid Animal Genomic DNA Extraction Kit $ B80047(BS47) EZ-0 Spin Column Animal Genomic DNA Mini-Preps Kit $ 44.0 B80047(BS68) EZ-0 Spin Column Animal Genomic DNA Mini-Preps Kit $ B80034(PT9034) EZ-500 Spin Column Animal Genomic DNA Maxi-Preps Kit 4 Preps $ 86.0 B80437(BS437) 96-Well Plate Animal Genomic DNA Mini-Preps Kit X Plates $ B80437(BS437) 96-Well Plate Animal Genomic DNA Mini-Preps Kit 5X Plates $ B58403(BS8403) One-Tube Clinical Sample DNA Extraction Kit 00 Preps $ B58403(BS8404) One-Tube Clinical Sample DNA Extraction Kit 500 Preps $ B80478(BT478) Rapid Blood Genomic DNA Extraction Kit $ B80483(BS483) EZ-0 Spin Column Blood Genomic DNA MiniPreps Kit $ 44.0 B80483(BS684) EZ-0 Spin Column Blood Genomic DNA MiniPreps Kit $ B8003(BT903) 96 Well Plate Blood Genomic DNA Mini-Prep Kit Plates $ B8003(BT903) 96 Well Plate Blood Genomic DNA Mini-Prep Kit 5 Plates $ B58409(VT4785) One Tube Viral DNA Isolation Buffer $ 5.50 B58409(VT4786) One Tube Viral DNA Isolation Buffer 00 Preps $ 0.00 B5867(VT88) EZ-0 Spin Column Viral DNA Mini-Preps Kit $ 7.40 B5867(VT883) EZ-0 Spin Column Viral DNA Mini-Preps Kit $ B8003(VT903) 96-Well Plate Viral DNA Mini-Preps Kit X Plates $ B58405(T76) One-Tube Swab DNA Extraction Kit 0 Preps $ BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

4 BBI Catalogue B58405(T763) One-Tube Swab DNA Extraction Kit 00 Preps $ 68.5 B58405(T764) One-Tube Swab DNA Extraction Kit 500 Preps $ B58407(BS8407) One-Tube Hair DNA Extraction Kit $ B68503(BS88503) ONE-4-ALL Genomic DNA Miniprep Kit $ 9.40 B68503(BS88505) ONE-4-ALL Genomic DNA Miniprep Kit DNA/RNA/Protein Isolation Kit $ Code B68003(BS88003) B6803(BS8803) RNA Isolation Kit Code B800409(BS409A) B800409(BS400A) B536(BS36) B5865(BS865) Description All-In-One DNA/RNA/Protein Miniprep Kit All-In-One DNA/RNA Miniprep Kit Description One Step RNA Reagent One Step RNA Reagent EZ-0 Spin Column Total RNA Mini-preps Kit Rapid Bacteria RNA Isolation Kit Size Size 5 ml 00 ml $ 0.5 $ $ 6.5 $ $ $ B60583(BS583) EZ-0 Spin Column Total RNA Mini-Preps Super Kit 0 Preps $ B60583(BS584) EZ-0 Spin Column Total RNA Mini-Preps Super Kit 00 Preps $ B60583(BS784) EZ-0 Spin Column Total RNA Mini-Preps Super Kit $ B80585(BS585) 96 Well Plate Bacterial Total RNA Mini-Preps Super Kit X Plates $ 6.50 B80585(BS585) 96 Well Plate Bacterial Total RNA Mini-Preps Super Kit 5X Plates $ B635(BS935) EZ-0 Spin Column RNA Cleanup & Concentration Kit $ 68.5 B805(BRC5) 96 Well Plate RNA Cleanup & Concentration Kit XPlates $ 6.50 B8046(FT746) Rapid Fungal RNA Extraction Kit $ B695(BS995) EZ-0 Spin Column Fungal RNA Mini-Preps Kit $ B8049(PT49) Rapid Plant RNA Isolation Kit $ B634(BS834) EZ-0 Spin Column Plant RNA Mini-Preps Kit $ B58637(VT484) Rapid Viral RNA Extraction Kit $ 7.40 B58667(VT8) EZ-0 Spin Column Viral Total RNA Extraction Kit $ B8048(AT48) Rapid Animal Total RNA Extraction Kit $ 5.50 B8048(AT48) Rapid Animal Total RNA Extraction Kit $ 0.00 B63(BS83) EZ-0 Spin Column Animal Total RNA Mini-Preps Kit $ 65.0 B63(BS83) EZ-0 Spin Column Animal Total RNA Mini-Preps Kit $ B8048(BT48) Rapid Blood RNA Isolation Kit 0 Preps $ 44.0 B8048(BT483) Rapid Blood RNA Isolation Kit $ B8048(BT484) Rapid Blood RNA Isolation Kit $ B633(BS833) EZ-0 Spin Column Blood RNA Mini-Preps Kit $ B8098(MT998) mrna Purification Kit from Total RNA 5 Preps $ 0.90 B68583(BS88583) EZ-0 Total RNA Miniprep Kit $ B6853(BS8853) Rnase-Free DNAse Set $ 3.30 B6833(BS8833) EZ-0 DNAway RNA Miniprep Kit $ 0.5 B6833(BS8836) EZ-0 DNAway RNA Miniprep Kit $ Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 59

5 More Kits Code Description Size A8000(9K ) Gel Extraction Kit (00 preps) 00 Preps $ 4.00 A8000(9K ) Gel Extraction Kit (00 preps) 00 Preps $ A830003(9K ) A830003(9K ) A80009(9K ) PCR Purification Kit (00 preps) PCR Purification Kit (00 preps) Plasmid Miniprep Kit (00 preps) 00 Preps 00 Preps 00 Preps $ $ $ A80009(9K ) Plasmid Miniprep Kit (00 preps) 00 Preps $ A80003(9K ) Genomic DNA Extraction. Blood Genomic DNA Prep Kit (400Rx) 400 Rxn $ A8004(9K ) Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (400Rx) 400 Rxn $ A80005(9K ) Genomic DNA Extraction. Plant Genomic DNA Prep Kit (400Rx) 400 Rxn $ A8006 (9K ) Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn $ A80007(9K ) Yeast Genomic DNA Extraction Prep Kit (400Rx) 400 Rxn $ A80003(9K ) Genomic DNA Extraction. Blood Genomic DNA Prep Kit (3300Rx) 3300 Rxn $ A8004(9K ) Genomic DNA Extraction. Bacteria Genomic DNA Prep Kit (3300Rx) 3300 Rxn $ A80005(9K ) Genomic DNA Extraction. Plant Genomic DNA Prep Kit (3300Rx) 3300 Rxn $8.00 A8006 (9K ) Animal Tissue & Fungi Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn $8.00 A80007(9K ) Yeast Genomic DNA Extraction Prep Kit (3300Rx) 3300 Rxn $8.00 A8003(9K ) Plasmid DNA Maxiprep (0 preps) 0 Preps $89.00 A8003(9K ) Plasmid DNA Midiprep (5 preps) 5 preps $ Columns & 96 Well Plates Code Description Size B65005(SD5005) EZ-0 Column & collection tube (blue tube, clear ring, clear collection) 00 $ 4.5 B65005(SD5005-EMPTY) EZ-0 Empty Column without filter, without collection tube & O-ring 000 $ 5.50 B65008(SD5008) EZ-0 RNA Column & collection tube(clear tube, clear ring, clear collection) 00 $ 6.5 B65006(SD5006) 96 well filter plate (960μl each well) $ B65006(SD5006-EMPTY) 96 well filter plate (no filter) $ 5.0 B65007(SD5007) 96 well DNA plate with membrane (960μl each well) $ F80896(BR58-96N) 96 WELL ML DEEP PLATE, NATURAL,EDGE FILLED, 0PLATES/BAG PK $ BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

6 Bio Basic Inc. EZ-Kits Overview BBI Catalogue Bio Basic Inc. supplies a remarkable variety of molecular biology kits, including Plasmid Miniprep, Gel Extraction, PCR Product Purification, and a complete line of Genomic DNA and RNA isolation kits offered in both spin column and 96-well plate formats! Although the detailed protocol for each kit varies, the general three-step principle remains the same: () BIND, () WASH, (3) ELUTE. Bio Basic s EZ-Membrane allows for fast and efficient purification, regardless of sample type, thereby providing you with high yields of purified product with minimal handling and effort. Spin Column Format EZ- 96-Well Plate Format Starting material Bind Wash () BIND Starting material is bound to Bio Basic s EZ-Membrane in spin column or plate format. () WASH The bound material is washed to remove impurities, buffers, and other unwanted materials. (3) ELUTE The purified product (DNA/RNA/Protein) is eluted from the membrane and collected for downstream application! Starting material Bind Wash Elute Purified product Elute Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 6

7 Plasmid DNA, Gel Extraction & PCR Products Purification Kits EZ-0 Spin Column PCR Products Purification Kit This kit utilizes membrane technology which selectively adsorbs up to 0 μg of PCR products or DNA fragments in the presence of a specialized binding buffer. Primers (<40-mer), nucleotides, enzymes, mineral oil and all other impurities are unable to bind and are eliminated from the column. PCR products or DNA fragments are eluted readily in elution buffer. PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. The kit can also be used for concentrating DNA fragments as well as removal of impurities such as salts and proteins.. Fast. Using a rapid spin column format, the entire procedure take less than 0 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B60363(BS363) B60363(BS364) B630663(BS664) 00 Preps Buffer B3 4 ml X4 ml 5X4 ml Wash Solution ml 4 ml 60 ml Elution Buffer 5 ml 0 ml 5 ml EZ-0 Column ml Collection Tube Gel electrophoresis of PCR products purified using EZ-0 Spin Column PCR Products Purification Kit. $36.75 $63.00 $ Well Plate PCR Products Purification Kit This kit provides a simple and efficient high throughput technique for the cleanup of PCR products and DNA fragments from gel extraction and enzymatic reaction solutions. PCR products or enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA is eluted with a small volume of low-salt elution buffer or water, and is ready for downstream applications including DNA sequencing and cloning.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure take less than 30 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Buffer B3 B80365 (BS365) x 96 well Plates 60 ml B80365(BS365) 5 x 96 well Plates X75 ml EZ-0 96-Well Plate 5 Deep Well Collection Plate Storage Plate 5 Sealing film 8 0 Complete $3.5 $ Reproducible yields of PCR products purified using the 96-Well Spin Column PCR Products Purification Kit. Lane : DNA marker. Lanes -5: Crude PCR products. Lanes 6 to 5: Purified PCR products. Recoveries and yields are approximately 80%. 6 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

8 BBI Catalogue EZ-0 Spin Column DNA Cleanup Mini-Preps Kit This kit provides a simple and efficient method for the purification of DNA fragments from various enzymatic reactions, including cdna synthesis, restriction digestions, and PCR, among others. It is also well-suited for desalting a solution of DNA, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures. The kit utilizes spin column technology which selectively adsorbs up to 0μg of DNA in the presence of specialized binding buffers. All impurities that do not bind are eliminated from the column. DNA fragments can be eluted readily with elution buffer. Purified DNA fragments and PCR products can be used for most downstream applications including sequencing and cloning.. Fast. Using a rapid spin column format, the entire procedure takes less than 0 minutes.. High Recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High Quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications, including digestion, cloning and sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. B60367(BS367) B60367(BS368) 00 Preps EZ-0 Column ml Collection Tube $36.75 $63.00 $ Well Plate DNA Cleanup Mini-Preps Kit B60367(BS668) Gel electrophoresis of PCR products purified using EZ-0 Spin Column DNA Cleanup Mini-preps Kit. This kit provides a simple and efficient high throughput method for the cleanup of PCR products and other DNA fragments from gel extraction solutions and enzymatic reactions. The kit utilizes a simple bind-wash-elute procedure. PCR products or other enzymatic reactions are mixed with a specialized binding buffer and pass through 96 columns of the plate. Whereas DNA binds to the column, impurities such as enzymes, salts, nucleotides and primers (< 40-mer) are washed away. Pure DNA can be eluted in a small volume of the low-salt elution buffer or water, ready for use in all downstream applications including DNA sequencing and cloning. Phenol/chloroform extraction and ethanol precipitation are not required. Recovering yield is typically 60-80%. Up to 0 μg of purified DNA fragments in each well can be obtained. It is suitable to recover 00 bp-40 kb DNA fragments.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes less than 30 minutes.. High recovery. For fragments of 00 bp-0 kb, >85% of DNA will be recovered. 3. High quality of DNA. Purified PCR products and DNA fragments can be used readily in all downstream applications including digestion, cloning and DNA sequencing. 4. Non-Toxic. No phenol/chloroform is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B80369 (BS369) x 96 well Plates B80369(BS369) 5 x 96 well Plates Clean Up Solution 60 ml X75 ml Elution Buffer ml 30 ml EZ-0 96-Well Plate 5 Deep Well Plate Well Storage Plate 5 Sealing film 8 $3.5 0 $6.50 Reproducible yields of high quality PCR products purified using the EZ-0 96-well Plate DNA Cleanup Mini-Prep Kit. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 63

9 EZ-0 Spin Column DNA Gel Extraction Kit This kit provides a simple and rapid method for the purification of DNA fragments from agarose gel. It is also well-suited for desalting, as well as the removal of residual organic solvents or unincorporated nucleotides or primers (<40-mer) from reaction mixtures containing DNA. The kit utilizes a spin column technology which selectively adsorbs up to 0 μg of DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the column and are eliminated. DNA fragments can be eluted readily in elution buffer and used for DNA sequencing, restriction digestion, and other downstream applications. Recovering yield ranges from 60 to 80%. No phenol/chloroform extraction or ethanol precipitation is required.. Fast. Using a rapid spin column format, the entire procedure takes less than 5 minutes.. High Recovery. Yield ranges from 60 to 80%. 3. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B60353(BS353) B60353(BS354) B630653(BS654) 00 Preps EZ-0 Column ml Collection tube DNA fragments purified from an agarose gel using the Gel Extraction Kit. $36.75 $63.00 $47.00 EZ-0 Spin Column Plasmid Mini-Preps Kit This kit provides a simple and efficient method for the purification of plasmid DNA from bacterial culture. Plasmid DNA is selectively adsorbed by the EZ-0 column, while impurities such as proteins, salts and nucleotides are washed away. In order to maximize the recovery yield of plasmid DNA, a color indicator VisualLyse - is added to the buffer, which prevents insufficient or over-lysis during the lysis and neutralization step. Note: This kit is designed for the preparation of up to 0 μg of plasmid DNA. If larger amounts are required, plasmid Maxi preps are recommended. For low copy plasmid, B6043(BS439), B6043(BS449) and B6043(BS649) are recommended. Solutions are doubled in low copy plasmid kits.. Fast. Using a rapid spin column format, the entire procedure takes only minutes.. High Purity of DNA. OD 60 /OD 80 ratio of purified DNA is.8-.9, and can be used directly in most downstream applications such as sequencing, PCR, transformation, restriction digestion and ligation. 3. Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 4. Highest yield is guaranteed by using VisulLyse reagent. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. B6043(BS43) B6043(BS44) 00 Preps B6043(BS64) EZ-0 Column ml Collection Tube $36.75 $63.00 $47.00 *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 0 C. ** Before use for the first time, add four volumes of 96%-00% ethanol to Wash Solution (v/v). Gel electrophoresis of plasmid DNA purified using the EZ-0 Plasmid DNA Mini-preps Kit. 64 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

10 BBI Catalogue Ultra-Fast Spin Column Plasmid DNA Mini-Preps Kit This kit facilitates single-step plasmid DNA purification from bacterial cultures. Using a one buffer system as opposed to the traditional three, the kit releases plasmid DNA rapidly from your sample. Although the yield of the recovered plasmid DNA is slightly lower than that from regular kits, it is much faster and simpler to use. Bacterial cultures are lysed and the lysates are directly loaded on to the spin column. Plasmid DNA is selectively bound onto the EZ-0 column and other impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water, and can be readily used for many downstream applications. This kit is suitable for the preparation of up to 8μg of pure plasmid DNA.. Fast and Simple: One step lysis of cell cultures. The entire procedure takes less than 0 minutes.. High Purity of DNA: OD 60 /OD 80 of plasmid DNA purified using this kit is >.8. Purified plasmid DNA can be used for many downstream applications, including DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic: phenol/chloroform extraction and ethanol precipitation are not required. 4. Direct manufacturer with most competitive price advantage. Transportation: At ambient room temperature. Lysis Buffer UF Buffer DW 4 ml Universal Wash Solution ml Elution Buffer 5 ml EZ-0 Column 50.0ml Collection Tube 50 $5.50 B604(BS804) 50 ml EZ-500 Spin Column Plasmid DNA Maxi-Preps Kit Plasmid DNA purified using the Ultra-Fast EZ-0 Spin Column Plasmid DNA Mini-preps Kit. This kit provides a simple and efficient method for purifying large amounts of plasmid DNA. Up to 500 μg of plasmid DNA is selectively adsorbed on the 5 ml EZ-500 column. Impurities, including proteins, salts and nucleotides, are eliminated from the column and washed away. Plasmid DNA can be eluted in 3 ml of Elution buffer. No phenol/chloroform or ethanol precipitations are required. DNA purified using the kit can be used in many downstream applications including transformation, DNA sequencing, PCR, and Southern-blotting. In order to obtain a maximal recovery yield, a color indicator - VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Specification: Sampling Yield Handling Time 00~50 ml of bacteria culture for high copy plasmids up to 500 µg ~ hours 00~400 ml of bacteria culture for low copy plasmids for high-copy plasmid Transportation: At ambient temperature. B60465(BS4654) B60465(BS466) 4 Preps 0 Preps MAXI Resuspension Solution 55 ml X0 ml MAXI Cell Lysis Solution 55 ml X0 ml MAXI 3 Neutralization Solution 55 ml X0 ml MAXI 4 Equilibration Solution 54 ml X35 ml MAXI 5 Washing Solution 4X55 ml 4X75 ml MAXI 6 Elution Solution 54 ml X35 ml RNase A (50 mg / ml) 88 μl 440 μl MAXI Column 4 0 Collection Tube 4 0 $75.60 $ Solutions provided in this kit contain irritants, wear gloves and lab coat when handling.. Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube content into MAXI Resuspension Solution bottle. Add 50 μl of MAXI Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI Resuspension Solution bottle. Store at 4 C. 3. Check MAXI Cell Lysis Solution before use. Warm MAXI Cell Lysis Solution at 37 C if any precipitation formed. Prevent vigorous shaking of the MAXI Cell Lysis Solution. 4. To avoid acidification of MAXI Cell Lysis Solution from CO in the air, close the bottle immediately after use. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 65

11 96-Well Plate Plasmid DNA Mini-Preps Kit This kit provides a rapid, high-throughput method for 96-well plasmid DNA purification. Plasmid DNA is selectively adsorbed on silica membranes in the 96-well plate. Proteins, salts, nucleotides and other contaminants are subsequently eliminated from the column. Purified plasmid DNA can then be eluted in a small volume of elution buffer. In order to obtain maximal yield, a color indicator VisualLyse - is added to the buffer which prevents insufficient or over-lysis during the lysis and neutralization step. Purified DNA can be used directly in a variety of applications including sequencing, PCR and Southern-blotting. Note: This kit is used for the preparation of a maximum of 0μg of pure plasmid DNA per well.. Fast. Using the rapid spin column and high-throughput format, the entire procedure takes only 30 minutes.. High DNA Yield. Up to 0 μg DNA in each well can be obtained. 3. High Purity of DNA. OD 60 /OD 80 ratio of purified DNA is typically Non-toxic. No phenol/chloroform extraction or ethanol precipitation is required. 5. Direct manufacturer with most competitive price advantage. Transportation: At ambient temperature. Solution I Solution III B8045 (BS45) 96 Well Plates 5 ml 90 ml B8045(BS45) 5 96 Well Plates 60 ml 0 ml EZ-0 96-Well Plate Deep Well Collection Plate 96 Storage Plate Sealing film 0 5 $3.5 $ *Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 C. ** Before use for the first time, add four volumes of 96%-00% ethanol to Wash Solution Reproducible yields of plasmid DNA purified using the 96-Well Plate Plasmid DNA Mini-preps Kit. Ultra-Fast 96-Well Plate Plasmid DNA Mini-Preps Kit This kit is designed for one-step plasmid DNA purification from bacterial culture using a rapid and high throughput spin column format. Using a one buffer solution and replacing the traditional three, the kit releases plasmid DNA rapidly from bacterial culture. Although the yield of the recovered plasmid DNA is slightly lower than that from regular 96-Well Plate Kits, it is simpler and faster. Bacterial cultures are lysed and the lysates are directly loaded to each column of the 96-Well plate. Plasmid DNA is selectively bound onto the 96-well plate and other impurities are washed away. Pure plasmid DNA is eluted in Elution buffer or water, and can be readily used for most downstream applications. This kit is used for preparation of up to 6-8μg of pure plasmid DNA.. Fast & Simple. Using one step lysis spin column and 96-well high throughput format, the entire procedure takes only 0 minutes.. High Purity of DNA. OD 60 /OD 80 of plasmid DNA purified by this kit is >.8. Purified plasmid DNA can be used for many downstream applications such as DNA sequencing, cloning, PCR, transformation and restriction digestions. 3. Non-toxic. No phenol/chloroform extraction and ethanol precipitation are required. Transportation: At ambient temperature. Lysis-Buffer-UF Buffer DW Universal Wash Solution II Elution Buffer EZ-0 96-Well Plate Deep Well Collection Plate 96 Storage Plate Sealing film B8008(BS908) 96 Well Plates 00 ml 00 ml 48 ml 0 ml 4 8 $3.5 B8008(BS909) 5 96 Well Plates X50 ml X0 ml X48 ml 50 ml $ BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

12 BBI Catalogue 96-Well Plate Single Colony Plasmid DNA Mini-Preps Kit This kit provides a simple, efficient and high throughput method for plasmid DNA purification. Plasmid DNA and DNA fragments are selectively adsorbed onto the EZ-0 columns of the 96-well plate. Other impurities such as proteins, salts and nucleotides are washed away. Plasmid DNA can then be eluted in a small volume of elution buffer. Purified plasmid DNA can be used for many downstream applications, including PCR, DNA sequencing, enzyme digestion and transformation. Note: the diameter of each colony should be > mm.. Fast. Using a rapid spin column and 96-well high throughput format, the entire procedure takes 0 minutes.. Direct amplification from colonies, or glycerol stock solutions, no overnight culture is required. 3. Suitable for fast and large number screening. 4. Compatible with all downstream applications including PCR, restriction analysis and DNA sequencing. 5. Economic. Transportation: at ambient room temperature PT Well Plates PT Well Plates puc plasmid DNA purified using the 96-well single colony plasmid DNA MiniPrep Kit. EZ-0 Spin Column Endotoxin-Free Plasmid DNA Mini-Preps Kit This kit provides a simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Greater than 90% of the endotoxin can be removed using our Endotoxin-Be-Gone reagent. If large scale preparation is needed, EZ-500 Column can be used (B549(SK49) & B549(SK50)). Purified endotoxin-free plasmid DNA is suitable for transfection and other downstream applications.. Fast and convenient. Endotoxin is removed at the bacteria stage and the remaining steps are regular plasmid DNA extraction steps.. Efficient. A single treatment removes 90% of the endotoxin. Repeat procedure for greater efficiency. Purified plasmid DNA can be used for many downstream applications including transfection. 3. Economic. Solution I Solution II Solution III Wash Solution Elution Buffer Liquid Endotoxin Eliminator EZ-0 Column RNase A Solution (0mg/ml).0ml Collection Tube B698(BS798) 6 ml ml 5 ml 0 ml 5 ml 5 ml 50 0 μl 50 $0.90 Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 67

13 EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit This kit provides a large-scale, simple and efficient method for high quality endotoxin-free plasmid DNA purification from bacterial culture. Up to 500ug of endotoxin-free plasmid DNA may be obtained per maxi prep. Greater than 90% of the endotoxin can be removed using bacterial endotoxin erasol at bacteria stage. Purified endotoxin-free plasmid DNA can be used for transfection and other downstream applications.. Fast. Using a maxi-prep spin-column format, the entire procedure takes only 40 minutes.. High Quality of DNA. >90-99% of endotoxin can be removed. 3. Economic. SK49 SK50 4 Maxi-Preps 0 Maxi-Preps MAXI Resuspension Solution 55 ml x0 ml MAXI Cell Lysis Solution 55 ml x0 ml MAXI 3 Neutralization Solution 55 ml x0 ml MAXI 4 Equilibration Solution 54 ml x35 ml MAXI 5 Washing Solution 4x55 ml 4X75 ml MAXI 6 Elution Solution 54 ml x35 μl Liquid Endotoxin Eliminator 8 ml 40 ml RNase A (50 mg / ml) 88 μl x0 μl MAXI Column 4 0 $86.0 $ EZ-0 Spin Column Yeast Plasmid DNA Mini-Preps Kit The kit provides a simple and efficient Spin Column approach for Mini plasmid DNA purification from yeast. Plasmid DNA of lysed yeast cells is selectively bound onto the column. Other impurities such as proteins and salts do not bind to the column and are eliminated in the flow through. Purified plasmid DNA can be used for PCR, transformation, restriction digestion and many other downstream applications.μg of plasmid DNA can be purified from 5 ml yeast cells.. Fast. Using a rapid spin-column format, the entire procedure takes approximately hours.. High Quality of DNA. OD 60 /OD 80 of purified DNA is generally > Compatible with most downstream applications such as PCR, restriction digestion and hybridization. 4. Non-Toxic. Phenol/chloroform extractions or ethanol precipitation is not required. 5. Direct manufacturer with most competitive price advantage. Transportation: at ambient temperature Buffer P Buffer P Buffer P3 Buffer DW Universal Wash Buffer Elution Buffer B60467(BS467) 0 Preps 3 ml 3 ml 4 ml 5 ml 3 ml ml B60467(BS468) 5 ml 5 ml 0 ml 5 ml ml 5 ml RNase A Solution (0mg/ml) 5 μ 0 Snailase Snailase Storage Buffer Snailase Reaction Buffer EZ-0 Spin Column.0ml Collection Tube 60 mg 0.5 ml 7 ml 0 0 $.00 B60467(BS469) 75 ml 75 ml 00 ml 5 ml 60 ml 5 ml 60 μl 500 mg.5 ml 75 ml $ l μl mg.5 ml 35 ml $ BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

14 BBI Catalogue Genomic DNA Purification & Extraction Kits EZ-DNA Reagent EZ-DNA Reagent is a ready-to-use reagent for the isolation of genomic DNA from various samples, including animal tissue, cultured cells, and bacteria. The method is based on the use of an optimized guanidine-detergent solution, which lysis the tissue quickly and selective precipitates DNA from a cell lysate. During the isolation step, tissue or cells are lysed in EZ-DNA Reagent and the genomic DNA is precipitated from the lysate with ethanol. Following an ethanol wash, purified DNA is dissolved in water, TE buffer or 8 mm NaOH. The protocol allows for fast isolation of genomic DNA from a large number of samples of small or large volume. The whole procedure takes about 0-30 minutes with DNA recovery of 70-90%. There is no need for overnight digestion, phenol extraction. Isolated DNA can be used directly for most applications such as Southern analysis, molecular cloning and PCR.. Fast. The entire procedure takes only 0-30 minutes.. Simple. One-Tube procedure, no phenol/chloroform extraction is required. 3. Versatile. Suitable for wide range of samples including animal tissue, cultured cells, and bacteria. 4. Easy to scale up. 5. Economic. Transportation: tambient temperature. EZ-DNA Reagent A8080(BS80) 00 preps 00 ml $5.50 DNA extraction from various species using EZ-DNA Reagent. M. DNA marker. Lane -3: Genomic DNA from Bacteria. Lane 4-6: Genomic DNA from Rat. Lane 7-9: Genomic DNA from Yeast. One-Tube Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative and Gram-positive bacteria. Purified DNA can be used for most downstream applications, including PCR, restriction digestion, and hybridization.. Fast. Te entire procedure takes less than 0 minutes.. Simple. No phenol/chloroform extraction or ethanol precipitation is required. 3. Economic. Transportation: At ambient temperature. B80843(BS843) 00 Preps B80843(BS844) 500 Preps $ $59.60 DNA isolated from various bacteria. M: DNA marker. Lane -3: Escherichia coli (Gram-Negative). Lane 4-6: Staphyloccocus aureus (Gram-Positive). Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 69

15 Rapid Bacteria Genomic DNA Isolation Kit The kit is designed for rapid small-scale extraction of high quality genomic DNA from a variety of Gram-negative or Gram-positive bacteria. Purified DNA can be used for many downstream applications such as PCR, restriction digestion, and hybridization.. Fast. It takes less than 30 minutes.. Simple. 3. Economic. Transportation: Room temperature Universal Digestion Buffer Buffer PB TE Buffer B585(BS85) 5 ml ml 0 ml $36.75 EZ-0 Spin Column Bacterial Genomic DNA Mini-Preps Kit This kit provides a simple and convenient silica membrane-based technique to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. Total hands-on preparation time is less than 30 minutes. Bacteria samples are lysed in an optimized buffer. Released DNA binds specifically to the column while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps. Pure DNA is then eluted in either water or a low-salt buffer provided with the kit. The kit is also suitable for isolation of bacterial genomic DNA from colonies on a dish. Purified genomic DNA is 0-50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications.. Fast. Using a rapid spin-column format, the entire procedure takes approx 0 minutes.. High yield. 5-0 μg of bacterial genomic DNA can be obtained from ml ( cells) of overnight culture. 3. High quality of DNA. OD 60/80 ratio of purified DNA is generally > Non-Toxic. No phenol extraction or ethanol precipitation is required. 5. Economic Transportation: At ambient temperature. B6043(BS43) B80064(BS64) EZ-0 Spin Column ml Collection Tube $44.0 $83.75 DNA isolated from various bacteria. M: DNA Marker Lane -3: Escherichia coli (Gram Negative) Lane 4-6: Staphyloccocus aureus (Gram Positive) 70 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

16 BBI Catalogue 96-Well Plate Bacterial Genomic DNA Mini-Preps Kit The kit provides a simple and convenient high throughput approach to isolate high quality DNA from both Gram-negative and Gram-positive bacteria. DNA in a cell lysate is selectively adsorbed on to the EZ-0 column while impurities such as proteins, salts and nucleotides are washed away. No phenol/chloroform extraction or ethanol precipitation is required. Purified genomic DNA can be up to 50 kb in length. Genomic DNA purified can be used for PCR, and other downstream applications.. Suitable for Gram-positive and Gram-negative bacteria.. Fast. Using a high throughput 96-well plate format. 3. High purity of DNA. A 60/80 ratio of purified DNA is generally > Compatible with downstream applications. Transportation: At ambient temperature. Elution Buffer 96-Well Plate Deep Well Collection Plate 96-Well Storage Plate Sealing film B809(SK9) 96 Well Plates 4 8 $59.60 B8095(SK95) 5 96 Well Plates $ E. coli genomic DNA purified using the 96-well plate mini-preps kit. Shown on 0.8% agarose with DNA ladders. EZ-500 Spin Column Bacteria Genomic DNA Maxi-Preps Kit This kit provides a simple and convenient technique for large-scale isolation of high quality DNA from both Gram-negative and Gram-positive bacteria. Up to 500 μg of DNA is selectively bound to EZ-500 spin column. Other impurities present in the lysate, including proteins and salts, do not bind on the column and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. The kit is also suitable for the isolation of bacterial genomic DNA from colonies grown on a dish. Purified genomic DNA can be up to 50 kb in length. Purified DNA is suitable for downstream applications such as restriction endonuclease digestions, PCR, and other applications. For isolation of DNA from Gram positive bacteria, bacteria should be pre-treated with a buffer containing lysozyme.. Suitable for both Gram-positive and Gram-negative bacteria.. High purity of DNA. Purified DNA has an A 60/80 ratio of between.6 and.9 3. Compatible with many downstream applications. 4. Direct manufacturer with most competitive price advantage. Universal Digestion Buffer Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer ( ph 9.0) Proteinase K (0mg/ml) Maxi Columns 50 ml Collection Tube B80038(PT9038) Maxi Preps 40 ml 50 ml 8 ml 7.5 ml 5 ml 5 ml 4 4 $ M E.coli genomic DNA purified using the EZ-500 Spin Column Bacteria DNAMaxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker -7: genomic DNA from E.coli Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 7

17 Rapid Yeast Genomic DNA Extraction Kit This kit is designed for rapid, small-scale extraction of high quality genomic DNA from yeast. Yeast cell walls are digested by Snailase (or lyticase, zymolyase) and the membrane is lysed in the presence of proprietary buffers. DNA is then precipitated and washed with alcohol. Purified DNA can be used for many downstream applications, including PCR, restriction digestion, and hybridization.. Fast. The entire procedure takes less than 40 minutes.. Easy to use. 3. Economic. Universal Digestion Buffer Buffer PY TE Buffer Snailase Reaction Buffer Snailase Storage Buffer Snailase B587(BS87) 5 ml ml 0 ml 75 ml 5 ml 600 mg $36.75 Genomic DNA isolated from Saccharomyces cerevisiae Lane -3: Genomic DNA from Saccharomyces cerevisiae. EZ-0 Spin Column Yeast Genomic DNA Mini-Preps Kit The kit provides a simple and convenient method for the isolation of high quality yeast DNA. DNA present in cell lysate is selectively bound to the spin column while other impurities such as proteins and salts do not bind and are eliminated in the flow through. Purified genomic DNA is 0-50 kb in length, and is suitable for many downstream applications, including restriction endonuclease digestion, PCR, and cloning.. Fast. Using a rapid spin-column format, the entire procedure takes approx 30 minutes.. High Quality of DNA: OD 60 /OD 80 of purified DNA is generally Non-Toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: At ambient temperature including Proteinase K and Snailase. Universal Digestion Buffer Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer ( ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase B80857(BS857) ml 6 ml ml 8 ml 7.5 ml 5 ml. ml 75 ml 5 ml EZ-0 Spin Column 50.0ml Collection Tube mg $60.90 Genomic DNA isolated from Saccharomycetaceae 7 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

18 BBI Catalogue 96-Well Plate Yeast Genomic DNA Mini-Preps Kit This kit provides a simple and convenient, high-throughput approach to isolate high quality DNA from yeast. DNA present in cell lysate is selectively adsorbed onto the EZ-0 column, while impurities such as proteins, salts and nucleotides are washed away. Purified genomic DNA can be up to 50 kb in length, and can be used for a wide array of downstream applications, including PCR and restriction endonuclease digestions.. Fast. using the rapid spin-column and high throughput format, the entire procedure takes only hours.. High quality of DNA. OD 60/80 of purified DNA is generally Non-toxic. No phenol extraction or ethanol precipitation is required. 4. Economic. Transportation: at ambient temperature (including Proteinase K and Snailase). Universal Buffer Digestion Universal Buffer PY Universal Buffer BD Universal PW Solution Universal Wash Solution CE Buffer (ph 9.0) Proteinase K Snailase Reaction Buffer Snailase Storage Buffer Snailase 96-Well EZ Plate 96-Well Collection Plate 6 96-Well Storage Plate Sealing film B808357(BS8357) 96 Well Plates 48 ml 4 ml 48 ml 7 ml 30 ml 5 ml 4.8 ml 300 ml 0 ml.4 g 8 $86.90 Saccharomyces cerevisiae genomic DNA purified using the 96-well plate Yeast Genomic DNA Mini-Prep Extraction Kit. Rapid Fungal Genomic DNA Isolation Kit This kit provides a simple and efficient method for the isolation of total DNA (genomic and mitochondrial) from fungal species. The size of genomic DNA purified by this procedure is around 0-50 kb. Purified DNA can be readily used in a number of downstream applications including RT-PCR, SNP analysis, and restriction digestion.. Fast. the entire procedure takes less than 40 minutes.. Easy. 3. Economic. Universal Digestion Buffer Universal Buffer PF TE Buffer B8045(FT745) 50 preps 5 ml ml 0 ml $36.75 DNA isolated from various fungal species M: Marker Lane -: Penicillium spp. Lane 3-4: Pleurotus ostreatus. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 73

19 EZ-0 Spin Column Fungal Genomic DNA Mini-Preps Kit This kit provides a simple and convenient method for the isolation of high quality DNA from various fungal species. DNA present in a cell lysate is selectively bound to the spin column, while other impurities such as proteins, salts, and nucelotides do not bind and are eliminated in the flow through. No phenol extraction or ethanol precipitation is required. Purified genomic DNA is 0-50 kb in length, and is suitable for downstream applications, including restriction digestions, cloning, and PCR.. Fast and Simple. Using a spin-column format, the entire procedure takes only 30 minutes.. High quality of Fungal DNA. 3. Economic. B800(FT80) 50 preps Universal Digestion Buffer ml Universal Buffer PF 6 ml Universal Buffer BD ml Universal PW Solution 8 ml Universal Wash Solution 7.5 ml TE Buffer (ph 8.0) 0 ml Proteinase K. ml Genomic DNA isolated from various fungi EZ-0 Spin Column 50 Lane -3: Penicillium spp. genomic DNA -ml Collection Tube 50 $44.0 Lane 4-6: Pleurotus ostreatus. DNA EZ-500 Spin Column Fungal Genomic DNA Maxi-Preps Kit This kit provides a simple and efficient method for the isolation of genomic DNA from a variety of fungal species. DNA present in a cell lysate is selectively adsorbed in the column while other impurities are eliminated. Average DNA yield is ~0-00μg per preparation. Average size of the genomic DNA purified by this procedure is around 0-50 kb. No phenol/chloroform extraction, ethanol precipitation, or liquid nitrogen homogenization is required in this protocol. Purified fungal genomic DNA can be used for PCR, SNP, RAPD, RFLP, AFLP, Southern blotting, transformation and other applications.. Fast.. High Quality of DNA. OD 60/80 ratio of purified fungal genomic DNA is generally > Suitable for various filamentous fungal species including S. cerevisiae, S. pombe, Pichia pastoris, Candida albicans, Aspergillus niger, Collectotrichum musae, and Fusarium oxysporum. Universal Digestion Buffer Universal Buffer PF Universal Buffer BD Universal PW Solution Universal Wash Solution TE Buffer (ph 8.0) Proteinase K EZ-500 Spin Columns 4 50-ml Collection Tube 4 B80037(PT9037) 4 Maxi Preps 50 ml 5 ml 50 ml 8 ml 7.5 ml 0 ml 5 ml $86.0 Purified fungal genomic DNA run on a 0.7% agarose gel. 74 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA

20 BBI Catalogue Rapid Plant Genomic DNA Isolation Kit This kit is designed for the rapid isolation of genomic DNA from plant leaves and seeds. Samples may be fresh, frozen, or dried. The whole procedure takes approximately 5 minutes and the kit is suitable for screening and high throughput extraction. No phenol/chloroform extraction or ethanol precipitation is required. Purified Genomic DNA can be used for RT-PCR, cloning and other downstream applications.. Fast. The entire procedure takes less than 30 minutes.. Easy. 3. Direct manufacturer with most competitive price advantage. B8086(PT786) 00 preps $5.50 B8086(PT787) 500 preps $0.00 High molecular genomic DNA isolated from various plant species using the Rapid Plant DNA Kit. Lane -: Genomic DNA from Nicotiana tabacum Lane 3-4: Genomic DNA from Arabidopsis thaliana Lane 5-6: Genomic DNA from Oryza sativa M: DNA marker. EZ-0 Spin Column Plant DNA Mini-Preps Kit This kit provides a simple and efficient method for the isolation of plant genomic DNA. Samples may be fresh, frozen, or dried. DNA in plant tissues & cells is selectively adsorbed on the spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Average size of genomic DNA purified by this procedure is ~30-50 kb. Purified genomic DNA can be used directly in PCR, Southern Blotting, RAPD, RFLP, and many more.. Fast. Using a rapid spin-column format, the entire procedure takes less than 30 minutes.. High Purity of DNA. OD 60/80 of purified DNA is generally > Compatible with many downstream applications such as PCR, restriction digestions, real-time PCR, multiplex PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 4. Suitable for a wide variety of plant species and tissue types including some recalcitrant specimens. B80045(BS45) 50 preps μ B80045(BS66) 50 preps μl EZ-0 Spin Column ml Collection Tube $44.0 $83.75 Genomic DNA isolated from various plant species using the EZ-0 Plant DNA mini-prep kit. Lane -: Nicotiana tabacum Lane 3-4: Arabidopsis thaliana Lane 5-6: Oryza sativa Lane 7-8: Platycladus orientalis Lane 9-0: Wisteria sinensis M: DNA marker Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA 75

21 EZ-500 Spin Column Plant Genomic DNA Maxi-Preps Kit B80035(PT9035) 4 preps/ B80035(PT9036) 0 preps The kit is designed for the isolation of large quantities of genomic DNA from a wide variety of plant species. Samples may be fresh, frozen, or dried. DNA in plant tissues and cells are selectively adsorbed on the EZ-500 maxi-prep spin column, while other impurities such as proteins, salts, and lipids do not bind to the column and are washed away. Up to 500 μg of plant DNA can be obtained per preparation. Average size of DNA purified using this procedure is ~30-50 kb. Purified genomic DNA can be used directly for PCR, Southern Blotting, RAPD, and more.. High Purity of DNA. OD 60/80 of purified DNA is generally >.8.. Compatible with many downstream applications, including such as PCR, RAPD, RFLP, AFLP, Southern Blotting and microsatellite analysis. 3. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. Transportation: At ambient temperature. 96-Well Plate Plant Genomic DNA Mini-Preps Kit E.coli genomic DNA purified using the EZ-500 Spin Column Plant DNA Maxi-Preps Kit. Shown on a 0.8% agarose gel. M: DNA Marker -7: genomic DNA from E.coli M This kit provides a rapid and convenient high-throughput technique for the preparation of high quality genomic DNA from various plant species. DNA in a plant lysate is selectively adsorbed on each column of the plate while other impurities such as proteins, salts, and lipids are eliminated from the column. Each column can adsorb up to 0 μg of DNA. No phenol/chloroform extraction or ethanol precipitation is required. Purified plant genomic DNA is 0-40 kb, and is suitable for an array of downstream applications including PCR, Southern blotting, microsatellite analysis, AFLP, RFLP, and RAPD.. Fast. Using a spin column format, the entire procedure takes less than hours.. High purity of DNA. OD 60/80 of purified DNA is generally > Compatible with many downstream applications. 4. Suitable for a wide variety of plant species and tissue types including some very recalcitrant specimens. B80836(BS836) 96 Well Plates Universal Buffer PCB 30 ml Universal Buffer BD 50 ml Universal PW Solution 7 ml Universal Wash Solution 30 ml TE Buffer (ph 8.0) 40 ml 96-Well EZ Plate 96-Well Collection Plate 6 96-Well Storage Plate Sealing film 8 $86.90 Plant genomic DNA purified using the 96-well Plate DNA Mini-Prep Kit. 76 BIO BASIC INC. Add: 0 Konrad Crescent, Markham, ON, L3R 8T4 Canada Tel:(905) , (800) Fax:(905) Bailey Avenue, Amherst, New York, 46 USA