J Chin Med 14(2): , 2003

Transcription

1 99 J Chin Med 14(2): , ( ) + galactosamine + + silymarin A B + 24 C aspartate aminotransferase; AST alanine aminotransferase; ALT p < 0.01 A A B hsumd736@ms67.hinet.net

2 100 B C 1 lamivudine 5 complementary and alternative med icine 2,3 ICR 20 ~ ± 1 C 60 ± 5 % / silymarin 15 / 75 mg 0.1 N NaOH / ICR /

3 / / silyamrin / 4. A / B 400 / C 400 / ( ) rpm 10 Sigma Diagnostics Transaminase reagents aspartate aminotransferase; AST alanine aminotransferase; ALT ( ) r1 + r2 R r1 + r2/r 50 % 25 ~ 50 % 25 % SPSS Nonparametric Statistics Mann-Whitney U Test Kruskal-Wallis H Test Dunnet t Test 400 / 40 /

4 aspartate aminotransferase; AST alanine aminotransferase; ALT 48 AST ALT ± / ± / AST ALT ± / ± 1.80 / Mann-Whitney U Test Figs.1, 2 Serum AST Activity (SF Units/ml) h 48h 0 A B C AST,p < 0.01,p < 0.01 Serum ALT Activity (SF Units/ml) h 48h 0 A B C ALT,p < 0.01, p < 0.01

5 103 silymarin AST ALT silymarin Figs.1, 2 24 AST C 36 Fig. 1, p < AST Fig. 1, p < 0.01 ALT 48 Fig. 2, p < 0.01 A AST ± 4.22 / ALT ± 3.50 / B AST ± / ALT ± 8.42 / 24 2 Kruskal-Wallis H Test Dunnet t Test B 24 C AST C ± 8.85 / B ± / 48 ALT C ± 3.03 / B ± 8.42 / Fig. 1, 2; p < 0.01 r1 + r2/r > 50 % r1 + r2/r < 25 % < 25 % r1 + r2/r < 50 % A Fig. 3 4,5 nucleotide RNA Milk Thistle 6, silibinin silidianin

6 104 Fig. 3-1 Fig. 3-2 Fig. 3-3 Fig. 3-4 A Fig. 3-5 B Fig. 3-6 C 3 A silicristin silymarin C 8

7 105 E 9 silymarin silymarin AST/ALT silymarin AST/ALT 36 C 24 B AST/ALT A B 24 A AST/ALT B A B C

8 106 13, Chang MH, Chen CJ, Lai MS, Hsu HM, Wu TC, Kong MS, Liang DC, Shau WY, Chen DS Universal hepatitis B vaccination in Taiwan and the incidence of hepatocellular carcinoma in children. N Engl J Med 336: , David M. Eisenberg Complementary and alternative medicine in the United states: overview and patterns of use. J Altern Complement Med: S19-21, David M. Eisenberg Complentary, Alternative and Integrative Medicine in the United States: Current Trends and Future Opportunities. The 2 nd World Integrative Medicine Congress, Beijing, Maxwell Afari Gyamfi, Masato Yonamine, Yoko Aniya Free-radical scavenging action of medicinal herbs from Ghana Thonningia sanguinea on experimentally-induced liver injuries. General Pharmacology. 32: , Adawi D, Ahrne S, Molin G Effects of different probiotic strains of Lactobacillus and Bifdobacterium on bacterial translocation and liver injury in an acute liver injury model. International J of Food Microbiology 70: , Wellington K, Jarvis B Silymarin: a review of its clinical properties in the management of hepatic disorders. Bio Drugs 15: , Saller R, Meier R, Brignoli R. The use of silymarin in the treatment of liver diseases. Drugs 61: , Bean P The use of alternative medicine in the treatment of hepatitis C. Am Clin Lab 21: 19-21, Lu KL, Tsai CC, Ho LK, Lin CC, Chang YS Preventive effect of the Taiwan folk medicine Ixerix laevigata var. oldhami on alpha-naphthyl-isothiocyanate and carbon tetrachloride-induced acute liver injury in rats. Phytother Res 16S1: S45-50, Purcell RH: Hepatitis viruses: Changing patterns of human disease. Proc Natl Acad Sci USA 91: , pp pp Niederau C, Heintges T, Erhardt A, Buschkamp M, Hurter D, Nawrocki M, Kruska L, Hensel F, Petry W, Haussinger D Prognosis of chronic hepatitis C: Results of a large, prospective cohort study. Hepatology 28: , 1998.

9 Poynard T Bedossa P, Opolon P Natural history of liver fibrosis progression in patients with chronic hepatitis C. Lancet 349: , Vento S, Garofano T, Renzini C, Cainelli F, Casali F, Ghironzi G, Ferraro T, Concia E Fulminant hepatitis associated with hepatitis A virus superinfection in patients with chronic hepatitis C. N Engl J Med 338: , 1998.

10 108 J Chin Med 14(2): , 2003 THE HEPATOPROTECTIVE EFFECT OF TRADITIONAL CHINESE MEDICINE IN GALATOSAMINE-INDUCED ACUTE HEPATOTOXICITY Min-Chien Yu 1, Chih-Hsiung Lee 4, Cheng-Wei Lai 4, I-Ping Chiang 3, Ying-Yu Wang 4 and Ching-Hsaing Hsu 2 1 Institute for integration of Traditional Chinese and Western Medicine 2 Department of Pediatric, 3 Department of Pathology China Medical College Hospital, Taichung, Taiwan 4 Gen Mont Biotechnology Incorportation Tainan, Taiwan (Received 28 th January 2003, revised Ms received 6 th March 2003, accepted 10 th March 2003) The mortality and morbidity of hepatoma were still high in the past few decades in Taiwan. Although viral hepatitis was known as the main reason; there are certain limitation in the treatment of hepatitis. Therefore, finding effective herbal medicine in the prevention of acute hepatitis maybe a valid approach. In our study, the mice were randomized into control group (intraperitoneal (i.p.) injection with normal saline and fed with normal saline), negative control group (i.p. injection with galatosamine and fed with normal saline), positive control group (i.p. injection with galatosamine and fed with silymarin), experimental group A (pretreated with 24X heptoprotective herbs; then i.p. injection with galatosamine followed with 24X heptoprotective herbs given), group B (i.p. injection with galatosamine and fed with 24X heptoprotective herbs) and group C (i.p. injection with galatosamine and fed with 36X heptoprotective herbs). Blood sampling were performed at the 24 th and 48 th hours after galatosamine induced acute hepatocellular damage. Serum level of aspartate aminotransferase and alanine aminotransferase were measured. Liver biopsy from right hepatic lobe was done for pathology. The results showed that the liver function was elevated in the negative control group compared with the normal control group. There was also severe necrosis of hepatocyte proved by the pathology. The result of positive control group showed partial reversed galatosamine induced acute hepatotoxicity. Three experimental groups could reduced galatosamine induced acute hepatotoxicity as the positive control group, es-pecially the group A. Besides, there are different effect under different kind of heptoprotective herbs concentration. Out results suggest that heptoprotective herbs could reduce galatosamine induced acute hepatotoxicity dose dependently. The preventive effect (group A) before galatosamine induced acute liver damage was better than other experimental groups. It required further study to understand the mechanism of such kind of heptoprotective herbs on the protection of hepatotoxicity. Key words: Heptoprotective herbs, Hepatotoxicity, Galatosamine. Correspondence to: Ching-Hsaing Hsu, Department of Pediatric,China Medical College Hospital, No. 2, Yuh Der Road, Taichung, Taiwan, Tel: ext. 4163, Fax: , hsumd736@ms67.hinet.net